Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014195

Physical Location & Seq

Genomic Coordinates Reverse (-)

16392317 .. 16393805

Gene Structure 1489 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1060300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGTCAGAAATATTTCAAACTTCTGTTAAACTCCCCATGCTAGACATTTCTCAGCCAGTATTGCACCCATCTTCTCTATCCTCTCTAGCTGAAGCCTTCAAAACATGGGGCTTCTTCCACATCACCAATCATGGGATCTCCAAAGATCTTTTCAGAAAACTATATTCTCTGTCAAATGACCTGTTCAACCTCCCTTCTGATACCAAACTCAAACTTGGTCCTTCCTCTTCTGCCAAAACTTATACCCCTCAATTCATAGCCTCTCCCTACTTTGAAAGTCTCAGAGTTTCTGGCCCCAAGTTCTTTGATTCTGCTCAAAGTTCTGCACAAGTTCTCTTTGACCAACACAACTCTGAATTCAGATTTTTCTTGAGTGAAATATTACAAGAATATGGGAGCAAGATGGCAGAACTATCGAAGAAGATCGTAAGGATTGCGCTGATGAGCTTGGGGGATGGATTGGAGAAGAAACATTACGAATCCGAATTCAAAAATTGCCATGGTTACTTGAGAGTGAACAACTACTCAACCCCTCCAGAATGTTTGGAAGATCAACATCAAGAGGTTGAAGGACTTGGAATGCACACTGACATGAGCTGTGTGACAATTGTGTACCAAGACGAAATCGGAGGGCTTCAAGTGAGATCAAAGGAGGGAAAGTGGATGCACATAACTCCATGTGAGGGGACCTTGGTGGTGAACATAGGAGACATGTTTCAAGCTTGGAGCAATGATAATTTGAGGTCATCAGAACATAGAGTCATTTTAAAGCAGCCGAAAAATCGGTTTTCGTTGGCCTTTTTTTGGTGTTTTGAGGATGAGAAGGTGATATTTGCACCAGAAGATGTGGTGGGAGAAGGGAATGAGAGGATTTACAAGCCATTTGTTTGCTTGGATTATTTGAAGTTCAGAGAGAGCAATGAGAGAGGGAAGTTCGAAAAAGTTGGGTTTACTGTGAGGGATTTTGCAGGGATCAAACACAAAATAACTAACTGTGAAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

335

Amino Acids

38.24

Weight (kDa)

6.14

Isoelectric Point (pI)

51.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	11 - 84	2.7e-15	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	171 - 270	2.5e-24	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014195)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23340 AT4G23340
fragaria_vesca	FvH4_7g14240
malus_domestica	MD01G1060300.v1.1 MD07G1137400.v1.1
prunus_persica	Prupe.2G170200_v2.0.a1
pyrus_communis	pycom01g09040
rosa_chinensis	RchiOBHm_Chr1g0355221
rosa_laevigata	RLG00000028170
rosa_multiflora	Rmu_sc0003115.1_g000042
rosa_roxburghii	Rroxscaffold_4G00300580
rosa_rugosa	Rorug01G0245600
rosa_samantha	Rh1AG258100 Rh1BG227700 Rh1CG241800 Rh1DG255500
rosa_wichuraiana	Rw1G022770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	2 cut(s)	143, 980
AcsI	RAATTY	2 cut(s)	356, 485
AcuI	CTGAAG	1 cut(s)	111
AfaI	GTAC	1 cut(s)	614
AfiI	CCNNNNNNNGG	3 cut(s)	132, 683, 804
AflIII	ACRYGT	1 cut(s)	711
AgsI	TTSAA	9 cut(s)	17, 100, 187, 275, 490, 569, 638, 719, 904
AjuI	GAANNNNNNNTTGG	2 cut(s)	134, 166
AluBI	AGCT	4 cut(s)	89, 447, 597, 722
AluI	AGCT	4 cut(s)	89, 447, 597, 722
Alw26I	GTCTC	2 cut(s)	284, 702
AlwI	GGATC	2 cut(s)	143, 980
AoxI	GGCC	2 cut(s)	292, 795
ApeKI	GCWGC	1 cut(s)	772
ApoI	RAATTY	2 cut(s)	356, 485
Asp700I	GAANNNNTTC	1 cut(s)	12
AspLEI	GCGC	1 cut(s)	439
AspS9I	GGNCC	3 cut(s)	218, 293, 687
AsuHPI	GGTGA	3 cut(s)	115, 709, 838
AsuII	TTCGAA	1 cut(s)	936
AvaII	GGWCC	2 cut(s)	218, 687
BarI	GAAGNNNNNNTAC	2 cut(s)	458, 490
BbvI	GCAGC	1 cut(s)	784
BccI	CCATC	3 cut(s)	76, 397, 449
BcgI	CGANNNNNNTGC	2 cut(s)	396, 430
BcoDI	GTCTC	2 cut(s)	284, 702
BfaI	CTAG	2 cut(s)	41, 86
BglII	AGATCT	1 cut(s)	145
BisI	GCNGC	1 cut(s)	773
BlsI	GCNGC	1 cut(s)	774
Bme18I	GGWCC	2 cut(s)	218, 687
BmgT120I	GGNCC	3 cut(s)	218, 293, 687
BmiI	GGNNCC	2 cut(s)	295, 688
BmsI	GCATC	1 cut(s)	654
BpmI	CTGGAG	1 cut(s)	519
Bpu14I	TTCGAA	1 cut(s)	936
BpuEI	CTTGAG	2 cut(s)	391, 529
BsaBI	GATNNNNATC	1 cut(s)	555
BsaJI	CCNNGG	2 cut(s)	499, 690
BsaXI	ACNNNNNCTCC	2 cut(s)	644, 674
Bsc4I	CCNNNNNNNGG	3 cut(s)	132, 683, 804
Bse1I	ACTGG	1 cut(s)	56
Bse3DI	GCAATG	2 cut(s)	736, 925
Bse8I	GATNNNNATC	1 cut(s)	555
BseDI	CCNNGG	2 cut(s)	499, 690
BseGI	GGATG	3 cut(s)	460, 669, 823
BseJI	GATNNNNATC	1 cut(s)	555
BseLI	CCNNNNNNNGG	3 cut(s)	132, 683, 804
BseMI	GCAATG	2 cut(s)	736, 925
BseMII	CTCAG	2 cut(s)	65, 295
BseNI	ACTGG	1 cut(s)	56
BseXI	GCAGC	1 cut(s)	784
BsgI	GTGCAG	1 cut(s)	309
BshFI	GGCC	2 cut(s)	294, 797
BslFI	GGGAC	1 cut(s)	700
BslI	CCNNNNNNNGG	3 cut(s)	132, 683, 804
BsmAI	GTCTC	2 cut(s)	284, 702
BsmFI	GGGAC	1 cut(s)	700
BsmI	GAATGC	1 cut(s)	585
BsnI	GGCC	2 cut(s)	294, 797
Bsp119I	TTCGAA	1 cut(s)	936
Bsp143I	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
Bsp19I	CCATGG	1 cut(s)	499
BspANI	GGCC	2 cut(s)	294, 797
BspCNI	CTCAG	2 cut(s)	64, 294
BspLI	GGNNCC	2 cut(s)	295, 688
BspPI	GGATC	2 cut(s)	143, 980
BspT104I	TTCGAA	1 cut(s)	936
BsrDI	GCAATG	2 cut(s)	736, 925
BsrI	ACTGG	1 cut(s)	56
BssECI	CCNNGG	2 cut(s)	499, 690
BssMI	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
BssT1I	CCWWGG	2 cut(s)	499, 690
Bst4CI	ACNGT	2 cut(s)	955, 995
Bst6I	CTCTTC	1 cut(s)	232
BstBI	TTCGAA	1 cut(s)	936
BstDEI	CTNAG	2 cut(s)	51, 281
BstDSI	CCRYGG	1 cut(s)	499
BstF5I	GGATG	3 cut(s)	460, 669, 823
BstHHI	GCGC	1 cut(s)	439
BstKTI	GATC	6 cut(s)	138, 148, 426, 553, 647, 975
BstMAI	GTCTC	2 cut(s)	284, 702
BstMBI	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
BstMWI	GCNNNNNNNGC	1 cut(s)	61
BstNSI	RCATGY	1 cut(s)	715
BstV1I	GCAGC	1 cut(s)	784
BstX2I	RGATCY	2 cut(s)	135, 145
BstYI	RGATCY	2 cut(s)	135, 145
BsuRI	GGCC	2 cut(s)	294, 797
BtgI	CCRYGG	1 cut(s)	499
BtsCI	GGATG	3 cut(s)	460, 669, 823
BtsIMutI	CAGTG	1 cut(s)	585
CfoI	GCGC	1 cut(s)	439
Cfr13I	GGNCC	3 cut(s)	218, 293, 687
Csp6I	GTAC	1 cut(s)	613
CviAII	CATG	7 cut(s)	37, 105, 131, 500, 592, 678, 712
CviQI	GTAC	1 cut(s)	613
DdeI	CTNAG	2 cut(s)	51, 281
DpnI	GATC	6 cut(s)	137, 147, 425, 552, 646, 974
DpnII	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
DraI	TTTAAA	1 cut(s)	768
Eam1104I	CTCTTC	1 cut(s)	232
EarI	CTCTTC	1 cut(s)	232
Eco130I	CCWWGG	2 cut(s)	499, 690
Eco47I	GGWCC	2 cut(s)	218, 687
Eco57I	CTGAAG	1 cut(s)	111
EcoO109I	RGGNCCY	1 cut(s)	687
EcoRI	GAATTC	2 cut(s)	356, 485
EcoT14I	CCWWGG	2 cut(s)	499, 690
ErhI	CCWWGG	2 cut(s)	499, 690
FaeI	CATG	7 cut(s)	40, 108, 134, 503, 595, 681, 715
FaqI	GGGAC	1 cut(s)	700
FatI	CATG	7 cut(s)	36, 104, 130, 499, 591, 677, 711
Fnu4HI	GCNGC	1 cut(s)	773
FokI	GGATG	3 cut(s)	467, 676, 830
Fsp4HI	GCNGC	1 cut(s)	773
FspBI	CTAG	2 cut(s)	41, 86
GlaI	GCGC	1 cut(s)	438
GluI	GCNGC	1 cut(s)	773
GsuI	CTGGAG	1 cut(s)	519
HaeIII	GGCC	2 cut(s)	294, 797
HhaI	GCGC	1 cut(s)	439
Hin1II	CATG	7 cut(s)	40, 108, 134, 503, 595, 681, 715
Hin6I	GCGC	1 cut(s)	437
HinP1I	GCGC	1 cut(s)	437
HindIII	AAGCTT	1 cut(s)	720
HinfI	GANTC	3 cut(s)	308, 479, 759
HphI	GGTGA	3 cut(s)	115, 709, 838
Hpy166II	GTNNAC	4 cut(s)	517, 613, 700, 951
Hpy188III	TCNNGA	3 cut(s)	370, 536, 560
Hpy8I	GTNNAC	4 cut(s)	517, 613, 700, 951
HpyAV	CCTTC	6 cut(s)	106, 204, 231, 563, 817, 851
HpyCH4III	ACNGT	2 cut(s)	955, 995
HpyCH4V	TGCA	6 cut(s)	64, 326, 583, 667, 836, 968
HpyF10VI	GCNNNNNNNGC	1 cut(s)	61
HpyF3I	CTNAG	2 cut(s)	51, 281
Hsp92II	CATG	7 cut(s)	40, 108, 134, 503, 595, 681, 715
HspAI	GCGC	1 cut(s)	437
Kzo9I	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
LmnI	GCTCC	2 cut(s)	396, 726
LpnPI	CCDG	6 cut(s)	69, 194, 276, 549, 852, 954
Lsp1109I	GCAGC	1 cut(s)	784
LweI	GCATC	1 cut(s)	654
MaeI	CTAG	2 cut(s)	41, 86
MaeIII	GTNAC	2 cut(s)	503, 601
MalI	GATC	6 cut(s)	137, 147, 425, 552, 646, 974
MboI	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
MboII	GAAGA	8 cut(s)	63, 106, 219, 430, 433, 478, 560, 854
MfeI	CAATTG	1 cut(s)	606
MflI	RGATCY	2 cut(s)	135, 145
MluCI	AATT	6 cut(s)	251, 356, 485, 493, 606, 736
MlyI	GAGTC	1 cut(s)	768
MroXI	GAANNNNTTC	1 cut(s)	12
MseI	TTAA	2 cut(s)	27, 767
MslI	CAYNNNNRTG	1 cut(s)	590
MunI	CAATTG	1 cut(s)	606
Mva1269I	GAATGC	1 cut(s)	585
MwoI	GCNNNNNNNGC	1 cut(s)	61
NcoI	CCATGG	1 cut(s)	499
NdeII	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
NlaIII	CATG	7 cut(s)	40, 108, 134, 503, 595, 681, 715
NlaIV	GGNNCC	2 cut(s)	295, 688
NmuCI	GTSAC	1 cut(s)	601
NspI	RCATGY	1 cut(s)	715
NspV	TTCGAA	1 cut(s)	936
PciI	ACATGT	1 cut(s)	711
PctI	GAATGC	1 cut(s)	585
PdmI	GAANNNNTTC	1 cut(s)	12
PfeI	GAWTC	2 cut(s)	308, 479
PkrI	GCNGC	1 cut(s)	774
PleI	GAGTC	1 cut(s)	767
PpsI	GAGTC	1 cut(s)	767
PpuMI	RGGWCCY	1 cut(s)	687
PscI	ACATGT	1 cut(s)	711
Psp5II	RGGWCCY	1 cut(s)	687
PspN4I	GGNNCC	2 cut(s)	295, 688
PspPI	GGNCC	3 cut(s)	218, 293, 687
PspPPI	RGGWCCY	1 cut(s)	687
PsuI	RGATCY	2 cut(s)	135, 145
RsaI	GTAC	1 cut(s)	614
RsaNI	GTAC	1 cut(s)	613
RseI	CAYNNNNRTG	1 cut(s)	590
SaqAI	TTAA	2 cut(s)	27, 767
SatI	GCNGC	1 cut(s)	773
Sau3AI	GATC	6 cut(s)	135, 145, 423, 550, 644, 972
Sau96I	GGNCC	3 cut(s)	218, 293, 687
SchI	GAGTC	1 cut(s)	768
SfaNI	GCATC	1 cut(s)	654
SfuI	TTCGAA	1 cut(s)	936
SinI	GGWCC	2 cut(s)	218, 687
SmiMI	CAYNNNNRTG	1 cut(s)	590
SmlI	CTYRAG	2 cut(s)	370, 508
SmoI	CTYRAG	2 cut(s)	370, 508
Sse9I	AATT	6 cut(s)	251, 356, 485, 493, 606, 736
SspI	AATATT	2 cut(s)	12, 381
SspMI	CTAG	2 cut(s)	41, 86
StyI	CCWWGG	2 cut(s)	499, 690
TaaI	ACNGT	2 cut(s)	955, 995
TaqI	TCGA	2 cut(s)	416, 936
TasI	AATT	6 cut(s)	251, 356, 485, 493, 606, 736
TfiI	GAWTC	2 cut(s)	308, 479
Tru1I	TTAA	2 cut(s)	27, 767
Tru9I	TTAA	2 cut(s)	27, 767
TscAI	CASTG	1 cut(s)	592
TseFI	GTSAC	1 cut(s)	601
TseI	GCWGC	1 cut(s)	772
Tsp45I	GTSAC	1 cut(s)	601
TspDTI	ATGAA	1 cut(s)	244
TspRI	CASTG	1 cut(s)	592
VpaK11BI	GGWCC	2 cut(s)	218, 687
XapI	RAATTY	2 cut(s)	356, 485
XceI	RCATGY	1 cut(s)	715
XmnI	GAANNNNTTC	1 cut(s)	12
XspI	CTAG	2 cut(s)	41, 86

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.