Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014195

Physical Location & Seq

Genomic Coordinates Forward (+)

20016661 .. 20017959

Gene Structure 1299 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1137400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGTCAGAAATATCTCAAACGTCTGTGAAACTCCCCATGCTAGACATTTCTCAGGCAATATTGGACCCATCTTCTCTATCCTCTCTAGCTGAAGCCTTCAAAACATGGGGCTTCTTCCACATCACCAATCACGGGATCTCCAAAGATCTTTTCAGAAAACTATATTCTCTGTCGAATGACCTTTTCAGCCTCCCTTCTGATACCAAACTCAAACTTGGCCCTTTGTCTTCTGCCAAAACTTATACCCCTCACTTCATAGCCTCTCCCTTCTTTGAAAGCCTCAGAGTTTCTGGCCCCAGGTTCTTTGATTCTGCTCAAACTTCTGCGCATGTTCTCTTTGACCAACACAACTCTGAATTCAGTGAAATATTACAGGAATATGGGAGCAAGATGGCGGAACTATCAAAGAAAATCGTAAGGATTGCTTTGATGAGCTTGGGGGAAGGATTGGAGAAGAAACATTACGAATCTGAATTCAAAAAGTGCCATGGTTACTTGAGAGTGAACAACTACTCGACCCCTCCAGAAAGTTTGGAAGATCAACATCAAGAAGTTGGACTTGGAATGCACACTGACATGAGCTGTGTGACGATTGTGTACCAAGACGAAATCGGAGGGCTTCAAGTGAGATCAAAGGAGGGAAAGTGGATGGACATAACTCCATGTGAGGGGACCTTGGTGGTGAACATAGGAGACATGTTTCAAGCTTGGAGCAATGAGAATTTGAGGTCATCAGAACATAGAGTCATTTTAAAGCAGCCGAAAAATCGCTTTTCGATGGCCTTTTTTGGTGTTTCGAGGATGAGAAGGCGATTTTTGCACCAGAAGATGTGGTGGGAGAAGAGCAATGAGAGAGGGAAGTTTGAAAAAGTTGGGTTTACTGTGAGGGATTTTGCTGGGATTAATCACCAAATTACCAAAGGTGACAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

311

Amino Acids

35.41

Weight (kDa)

8.31

Isoelectric Point (pI)

49.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	11 - 82	1.9e-14	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	167 - 263	3.4e-25	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014195)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23340 AT4G23340
fragaria_vesca	FvH4_7g14240
malus_domestica	MD01G1060300.v1.1 MD07G1137400.v1.1
prunus_persica	Prupe.2G170200_v2.0.a1
pyrus_communis	pycom01g09040
rosa_chinensis	RchiOBHm_Chr1g0355221
rosa_laevigata	RLG00000028170
rosa_multiflora	Rmu_sc0003115.1_g000042
rosa_roxburghii	Rroxscaffold_4G00300580
rosa_rugosa	Rorug01G0245600
rosa_samantha	Rh1AG258100 Rh1BG227700 Rh1CG241800 Rh1DG255500
rosa_wichuraiana	Rw1G022770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	327
AciI	CCGC	1 cut(s)	395
AclWI	GGATC	1 cut(s)	143
AcsI	RAATTY	3 cut(s)	356, 473, 721
AcuI	CTGAAG	1 cut(s)	111
AfaI	GTAC	1 cut(s)	599
AfiI	CCNNNNNNNGG	2 cut(s)	132, 668
AflIII	ACRYGT	1 cut(s)	696
AgsI	TTSAA	6 cut(s)	100, 275, 478, 623, 704, 866
AjnI	CCWGG	1 cut(s)	296
AjuI	GAANNNNNNNTTGG	4 cut(s)	134, 166, 543, 575
AluBI	AGCT	4 cut(s)	89, 435, 582, 707
AluI	AGCT	4 cut(s)	89, 435, 582, 707
Alw26I	GTCTC	1 cut(s)	687
AlwI	GGATC	1 cut(s)	143
AoxI	GGCC	3 cut(s)	217, 292, 780
ApeKI	GCWGC	1 cut(s)	757
ApoI	RAATTY	3 cut(s)	356, 473, 721
AseI	ATTAAT	1 cut(s)	903
AspLEI	GCGC	1 cut(s)	328
AspS9I	GGNCC	4 cut(s)	64, 218, 293, 672
AsuHPI	GGTGA	3 cut(s)	115, 694, 899
AvaII	GGWCC	2 cut(s)	64, 672
BarI	GAAGNNNNNNTAC	2 cut(s)	446, 478
BbsI	GAAGAC	1 cut(s)	219
BbvI	GCAGC	1 cut(s)	769
BccI	CCATC	4 cut(s)	76, 385, 643, 772
BciT130I	CCWGG	1 cut(s)	298
BcoDI	GTCTC	1 cut(s)	687
BfaI	CTAG	2 cut(s)	41, 86
BglII	AGATCT	1 cut(s)	145
BisI	GCNGC	1 cut(s)	758
BlsI	GCNGC	1 cut(s)	759
Bme1390I	CCNGG	1 cut(s)	298
Bme18I	GGWCC	2 cut(s)	64, 672
BmgT120I	GGNCC	4 cut(s)	64, 218, 293, 672
BmiI	GGNNCC	3 cut(s)	66, 295, 673
BmrFI	CCNGG	1 cut(s)	298
BpiI	GAAGAC	1 cut(s)	219
BpmI	CTGGAG	1 cut(s)	507
BpuEI	CTTGAG	1 cut(s)	517
BsaBI	GATNNNNATC	1 cut(s)	543
BsaJI	CCNNGG	3 cut(s)	296, 487, 675
BsaXI	ACNNNNNCTCC	4 cut(s)	122, 152, 629, 659
Bsc4I	CCNNNNNNNGG	2 cut(s)	132, 668
Bse3DI	GCAATG	2 cut(s)	721, 853
Bse8I	GATNNNNATC	1 cut(s)	543
BseBI	CCWGG	1 cut(s)	298
BseDI	CCNNGG	3 cut(s)	296, 487, 675
BseGI	GGATG	2 cut(s)	654, 807
BseJI	GATNNNNATC	1 cut(s)	543
BseLI	CCNNNNNNNGG	2 cut(s)	132, 668
BseMI	GCAATG	2 cut(s)	721, 853
BseMII	CTCAG	2 cut(s)	65, 295
BseXI	GCAGC	1 cut(s)	769
BseYI	CCCAGC	1 cut(s)	896
BshFI	GGCC	3 cut(s)	219, 294, 782
BslFI	GGGAC	1 cut(s)	685
BslI	CCNNNNNNNGG	2 cut(s)	132, 668
BsmAI	GTCTC	1 cut(s)	687
BsmFI	GGGAC	1 cut(s)	685
BsmI	GAATGC	1 cut(s)	570
BsnI	GGCC	3 cut(s)	219, 294, 782
Bsp143I	GATC	4 cut(s)	135, 145, 538, 629
Bsp19I	CCATGG	1 cut(s)	487
BspACI	CCGC	1 cut(s)	395
BspANI	GGCC	3 cut(s)	219, 294, 782
BspCNI	CTCAG	2 cut(s)	64, 294
BspLI	GGNNCC	3 cut(s)	66, 295, 673
BspPI	GGATC	1 cut(s)	143
BspQI	GCTCTTC	1 cut(s)	836
BsrDI	GCAATG	2 cut(s)	721, 853
BssECI	CCNNGG	3 cut(s)	296, 487, 675
BssMI	GATC	4 cut(s)	135, 145, 538, 629
BssT1I	CCWWGG	2 cut(s)	487, 675
Bst2UI	CCWGG	1 cut(s)	298
Bst4CI	ACNGT	2 cut(s)	883, 929
Bst6I	CTCTTC	1 cut(s)	836
BstDEI	CTNAG	2 cut(s)	51, 281
BstDSI	CCRYGG	1 cut(s)	487
BstF5I	GGATG	2 cut(s)	654, 807
BstHHI	GCGC	1 cut(s)	328
BstKTI	GATC	4 cut(s)	138, 148, 541, 632
BstMAI	GTCTC	1 cut(s)	687
BstMBI	GATC	4 cut(s)	135, 145, 538, 629
BstMWI	GCNNNNNNNGC	1 cut(s)	817
BstNI	CCWGG	1 cut(s)	298
BstNSI	RCATGY	2 cut(s)	332, 700
BstSCI	CCNGG	1 cut(s)	296
BstV1I	GCAGC	1 cut(s)	769
BstV2I	GAAGAC	1 cut(s)	219
BstX2I	RGATCY	2 cut(s)	135, 145
BstYI	RGATCY	2 cut(s)	135, 145
BsuRI	GGCC	3 cut(s)	219, 294, 782
BtgI	CCRYGG	1 cut(s)	487
BtsCI	GGATG	2 cut(s)	654, 807
BtsIMutI	CAGTG	2 cut(s)	367, 570
CfoI	GCGC	1 cut(s)	328
Cfr13I	GGNCC	4 cut(s)	64, 218, 293, 672
Csp6I	GTAC	1 cut(s)	598
CviAII	CATG	7 cut(s)	37, 105, 329, 488, 577, 663, 697
CviQI	GTAC	1 cut(s)	598
DdeI	CTNAG	2 cut(s)	51, 281
DpnI	GATC	4 cut(s)	137, 147, 540, 631
DpnII	GATC	4 cut(s)	135, 145, 538, 629
DraI	TTTAAA	1 cut(s)	753
Eam1104I	CTCTTC	1 cut(s)	836
EarI	CTCTTC	1 cut(s)	836
EciI	GGCGGA	1 cut(s)	410
Eco130I	CCWWGG	2 cut(s)	487, 675
Eco47I	GGWCC	2 cut(s)	64, 672
Eco57I	CTGAAG	1 cut(s)	111
EcoO109I	RGGNCCY	1 cut(s)	672
EcoRI	GAATTC	2 cut(s)	356, 473
EcoRII	CCWGG	1 cut(s)	296
EcoT14I	CCWWGG	2 cut(s)	487, 675
ErhI	CCWWGG	2 cut(s)	487, 675
FaeI	CATG	7 cut(s)	40, 108, 332, 491, 580, 666, 700
FalI	AAGNNNNNCTT	4 cut(s)	409, 441, 543, 575
FaqI	GGGAC	1 cut(s)	685
FatI	CATG	7 cut(s)	36, 104, 328, 487, 576, 662, 696
Fnu4HI	GCNGC	1 cut(s)	758
FokI	GGATG	2 cut(s)	661, 814
Fsp4HI	GCNGC	1 cut(s)	758
FspBI	CTAG	2 cut(s)	41, 86
FspI	TGCGCA	1 cut(s)	327
GlaI	GCGC	1 cut(s)	327
GluI	GCNGC	1 cut(s)	758
GsaI	CCCAGC	1 cut(s)	900
GsuI	CTGGAG	1 cut(s)	507
HaeIII	GGCC	3 cut(s)	219, 294, 782
HhaI	GCGC	1 cut(s)	328
Hin1II	CATG	7 cut(s)	40, 108, 332, 491, 580, 666, 700
Hin6I	GCGC	1 cut(s)	326
HinP1I	GCGC	1 cut(s)	326
HindIII	AAGCTT	1 cut(s)	705
HinfI	GANTC	3 cut(s)	308, 467, 744
HphI	GGTGA	3 cut(s)	115, 694, 899
Hpy166II	GTNNAC	4 cut(s)	505, 598, 685, 879
Hpy188I	TCNGA	8 cut(s)	7, 155, 199, 284, 355, 472, 614, 736
Hpy188III	TCNNGA	2 cut(s)	524, 548
Hpy8I	GTNNAC	4 cut(s)	505, 598, 685, 879
HpyAV	CCTTC	5 cut(s)	106, 204, 277, 437, 801
HpyCH4III	ACNGT	2 cut(s)	883, 929
HpyCH4IV	ACGT	1 cut(s)	20
HpyCH4V	TGCA	2 cut(s)	568, 820
HpyF10VI	GCNNNNNNNGC	1 cut(s)	817
HpyF3I	CTNAG	2 cut(s)	51, 281
HpySE526I	ACGT	1 cut(s)	20
Hsp92II	CATG	7 cut(s)	40, 108, 332, 491, 580, 666, 700
HspAI	GCGC	1 cut(s)	326
Kzo9I	GATC	4 cut(s)	135, 145, 538, 629
LguI	GCTCTTC	1 cut(s)	836
LmnI	GCTCC	2 cut(s)	384, 711
LpnPI	CCDG	8 cut(s)	38, 276, 283, 310, 359, 537, 836, 882
Lsp1109I	GCAGC	1 cut(s)	769
MaeI	CTAG	2 cut(s)	41, 86
MaeII	ACGT	1 cut(s)	20
MaeIII	GTNAC	3 cut(s)	491, 586, 923
MalI	GATC	4 cut(s)	137, 147, 540, 631
MboI	GATC	4 cut(s)	135, 145, 538, 629
MboII	GAAGA	7 cut(s)	63, 106, 219, 466, 548, 838, 853
MflI	RGATCY	2 cut(s)	135, 145
MluCI	AATT	4 cut(s)	356, 473, 721, 912
MlyI	GAGTC	1 cut(s)	753
MmeI	TCCRAC	1 cut(s)	535
MseI	TTAA	3 cut(s)	752, 903, 931
MslI	CAYNNNNRTG	1 cut(s)	575
MspR9I	CCNGG	1 cut(s)	298
Mva1269I	GAATGC	1 cut(s)	570
MvaI	CCWGG	1 cut(s)	298
MwoI	GCNNNNNNNGC	1 cut(s)	817
NcoI	CCATGG	1 cut(s)	487
NdeII	GATC	4 cut(s)	135, 145, 538, 629
NlaIII	CATG	7 cut(s)	40, 108, 332, 491, 580, 666, 700
NlaIV	GGNNCC	3 cut(s)	66, 295, 673
NmuCI	GTSAC	2 cut(s)	586, 923
NsbI	TGCGCA	1 cut(s)	327
NspI	RCATGY	2 cut(s)	332, 700
PciI	ACATGT	1 cut(s)	696
PciSI	GCTCTTC	1 cut(s)	836
PctI	GAATGC	1 cut(s)	570
PfeI	GAWTC	2 cut(s)	308, 467
PkrI	GCNGC	1 cut(s)	759
PleI	GAGTC	1 cut(s)	752
PpsI	GAGTC	1 cut(s)	752
PpuMI	RGGWCCY	1 cut(s)	672
PscI	ACATGT	1 cut(s)	696
PshBI	ATTAAT	1 cut(s)	903
Psp5II	RGGWCCY	1 cut(s)	672
Psp6I	CCWGG	1 cut(s)	296
PspFI	CCCAGC	1 cut(s)	896
PspGI	CCWGG	1 cut(s)	296
PspN4I	GGNNCC	3 cut(s)	66, 295, 673
PspPI	GGNCC	4 cut(s)	64, 218, 293, 672
PspPPI	RGGWCCY	1 cut(s)	672
PsuI	RGATCY	2 cut(s)	135, 145
RsaI	GTAC	1 cut(s)	599
RsaNI	GTAC	1 cut(s)	598
RseI	CAYNNNNRTG	1 cut(s)	575
SapI	GCTCTTC	1 cut(s)	836
SaqAI	TTAA	3 cut(s)	752, 903, 931
SatI	GCNGC	1 cut(s)	758
Sau3AI	GATC	4 cut(s)	135, 145, 538, 629
Sau96I	GGNCC	4 cut(s)	64, 218, 293, 672
SchI	GAGTC	1 cut(s)	753
ScrFI	CCNGG	1 cut(s)	298
SinI	GGWCC	2 cut(s)	64, 672
SmiMI	CAYNNNNRTG	1 cut(s)	575
SmlI	CTYRAG	1 cut(s)	496
SmoI	CTYRAG	1 cut(s)	496
Sse9I	AATT	4 cut(s)	356, 473, 721, 912
SsiI	CCGC	1 cut(s)	395
SspI	AATATT	2 cut(s)	60, 369
SspMI	CTAG	2 cut(s)	41, 86
StyD4I	CCNGG	1 cut(s)	296
StyI	CCWWGG	2 cut(s)	487, 675
TaaI	ACNGT	2 cut(s)	883, 929
TaiI	ACGT	1 cut(s)	23
TaqI	TCGA	4 cut(s)	173, 515, 776, 797
TasI	AATT	4 cut(s)	356, 473, 721, 912
TfiI	GAWTC	2 cut(s)	308, 467
Tru1I	TTAA	3 cut(s)	752, 903, 931
Tru9I	TTAA	3 cut(s)	752, 903, 931
TscAI	CASTG	2 cut(s)	367, 577
TseFI	GTSAC	2 cut(s)	586, 923
TseI	GCWGC	1 cut(s)	757
Tsp45I	GTSAC	2 cut(s)	586, 923
TspDTI	ATGAA	1 cut(s)	244
TspRI	CASTG	2 cut(s)	367, 577
VpaK11BI	GGWCC	2 cut(s)	64, 672
VspI	ATTAAT	1 cut(s)	903
XapI	RAATTY	3 cut(s)	356, 473, 721
XceI	RCATGY	2 cut(s)	332, 700
XspI	CTAG	2 cut(s)	41, 86

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.