MD01G1080100.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014124
Physical Location & Seq
Reverse (-)
18731503 .. 18734456
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1080100.v1.1.491

Sequence Viewer

Length: 963 bp
ATGCCTCCGAAGCGGAAGAGAACCACCGTCGCTCTGCCACGGCCTCCGCCGATGCAAAAGCGCATGCGAAGAGCCAAGACGATTTGCTCTTGCAGCTCCCCTCGCTTCACTCGCAGCTCCGCTCGCTCTGCCTTCTGCTGCTACGAAGAGGACGTTTGGACAGAGATCGCCAATCATCTAGACGCCAAGTCACTGGTGATGCTCGCGACGACATGTCGTTGGTTCCGCGGTCTGATCATGGAAGAAGGGAGCATATGGAAGCACGTGTGCCTACGTGATCTTCAGGTCCCTGAGCCTCGTCACGTGGCACTCAATTGGAGGAAGCTCTACGTTTCAGCCTTTGATGGGAGTCACTCTTACATGTTTCGCCAGCAGGAGAAGCATATCGACTGGATGCGCATCGGTGCATTCTCCGTTGAATCCTCAGAAGCATTCCTAACAGAAAAGTTGATCAAACCATCAAGGCTCCCAGAAGGAGATACCATACAGAAAATGTTGGAGTCCTGCGGCAGTTGTGTGTTGGATAAAGTTAGAACTGGGATATGGATTGCTGATCTCCAGCTTGTCCGATGCCCCGTCTGCGAGCTCAATACCTGTGATGGAACGATGCAGACATTGGATGCAAGGCATATTGAGCTGTTCTTAAGCGAGGGGTACAAGAATGGAAGCTGGGAATACGAACTTATAGGTCGCCATGATGTCAACGAGCATGCCGATGGTGCTTGTGGGGCTATTTTCGATATGAAACACCTCAAGGAATCTTCAACATCTGCAGTGTTTAATCTCAAGTCATGGGTGGGCAAACCAACAGACTGGCAACCAAAGGCTATCATTACTCTACATGCAGTTGCAGTCAACACAAATCTGCAGAAGAACGAAGGACTTCAAGTGAAATACCATGCCATGAGAGATGGACCTCAAGGAGAAGTAGTATCCATCAGAATCTCACAGCAGCTCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

321

Amino Acids

36.51

Weight (kDa)

8.78

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 51 - 92 3.9e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61730 AT5G36000
fragaria_vesca FvH4_7g16360 FvH4_7g16360
malus_domestica MD01G1080100.v1.1 MD07G1149100.v1.1
prunus_persica Prupe.2G187500_v2.0.a1
pyrus_communis pycom01g10840
rosa_chinensis RchiOBHm_Chr1g0359371
rosa_laevigata RLG00000027875
rosa_multiflora Rmu_co8499881.1_g000001
rosa_rugosa Rorug01G0270600
rosa_samantha Rh1AG284100 Rh1BG250200 Rh1CG268100 Rh1DG279100
rosa_wichuraiana Rw1G025220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 398
AccBSI CCGCTC 1 cut(s) 122
AccII CGCG 2 cut(s) 206, 228
AciI CCGC 6 cut(s) 13, 47, 120, 226, 228, 507
AcuI CTGAAG 1 cut(s) 266
AcvI CACGTG 2 cut(s) 265, 304
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 656
AfiI CCNNNNNNNGG 1 cut(s) 345
AflII CTTAAG 1 cut(s) 643
AflIII ACRYGT 3 cut(s) 212, 264, 360
AgsI TTSAA 3 cut(s) 419, 765, 887
AhdI GACNNNNNGTC 2 cut(s) 187, 213
AluBI AGCT 8 cut(s) 96, 117, 325, 562, 586, 637, 669, 955
AluI AGCT 8 cut(s) 96, 117, 325, 562, 586, 637, 669, 955
Alw21I GWGCWC 1 cut(s) 588
AlwNI CAGNNNCTG 1 cut(s) 958
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 4 cut(s) 93, 114, 138, 952
Asp700I GAANNNNTTC 1 cut(s) 882
AspLEI GCGC 2 cut(s) 63, 399
AspS9I GGNCC 2 cut(s) 286, 914
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 2 cut(s) 286, 914
BanII GRGCYC 1 cut(s) 588
BbrPI CACGTG 2 cut(s) 265, 304
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 3 cut(s) 105, 125, 126
BccI CCATC 6 cut(s) 338, 466, 593, 710, 905, 944
BceAI ACGGC 1 cut(s) 56
BciVI GTATCC 1 cut(s) 943
BclI TGATCA 2 cut(s) 234, 450
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 2 cut(s) 771, 866
BfrI CTTAAG 1 cut(s) 643
BfuI GTATCC 1 cut(s) 943
BisI GCNGC 5 cut(s) 94, 115, 139, 508, 953
BlsI GCNGC 5 cut(s) 95, 116, 140, 509, 954
Bme18I GGWCC 2 cut(s) 286, 914
BmeRI GACNNNNNGTC 2 cut(s) 187, 213
BmgT120I GGNCC 2 cut(s) 286, 914
BmiI GGNNCC 3 cut(s) 224, 288, 467
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 7 cut(s) 42, 189, 384, 408, 560, 597, 610
BmuI ACTGGG 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 542
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 3 cut(s) 737, 770, 903
BsaAI YACGTR 3 cut(s) 265, 275, 304
BsaBI GATNNNNATC 1 cut(s) 398
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 38, 226
BsaXI ACNNNNNCTCC 3 cut(s) 915, 939, 945
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse1I ACTGG 4 cut(s) 198, 395, 541, 818
Bse8I GATNNNNATC 1 cut(s) 398
BseDI CCNNGG 2 cut(s) 38, 226
BseGI GGATG 2 cut(s) 399, 625
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 282, 438
BseNI ACTGG 4 cut(s) 198, 395, 541, 818
BseXI GCAGC 3 cut(s) 105, 125, 126
BseYI CCCAGC 1 cut(s) 669
Bsh1236I CGCG 2 cut(s) 206, 228
BshFI GGCC 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 588
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 2 cut(s) 407, 431
BsnI GGCC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 5 cut(s) 165, 234, 277, 450, 553
Bsp68I TCGCGA 1 cut(s) 206
BspACI CCGC 6 cut(s) 13, 47, 120, 226, 228, 507
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 2 cut(s) 283, 437
BspFNI CGCG 2 cut(s) 206, 228
BspLI GGNNCC 3 cut(s) 224, 288, 467
BspMAI CTGCAG 2 cut(s) 775, 870
BspQI GCTCTTC 1 cut(s) 64
BspTI CTTAAG 1 cut(s) 643
BsrBI CCGCTC 1 cut(s) 122
BsrI ACTGG 4 cut(s) 198, 395, 541, 818
BssECI CCNNGG 2 cut(s) 38, 226
BssMI GATC 5 cut(s) 165, 234, 277, 450, 553
BssNI GRCGYC 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 3 cut(s) 11, 64, 141
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 643
BstBAI YACGTR 3 cut(s) 265, 275, 304
BstC8I GCNNGC 6 cut(s) 65, 124, 204, 371, 584, 711
BstDEI CTNAG 2 cut(s) 291, 424
BstDSI CCRYGG 2 cut(s) 38, 226
BstF5I GGATG 2 cut(s) 399, 625
BstFNI CGCG 2 cut(s) 206, 228
BstHHI GCGC 2 cut(s) 63, 399
BstKTI GATC 5 cut(s) 168, 237, 280, 453, 556
BstMBI GATC 5 cut(s) 165, 234, 277, 450, 553
BstNSI RCATGY 5 cut(s) 67, 216, 364, 713, 845
BstSFI CTRYAG 2 cut(s) 771, 866
BstUI CGCG 2 cut(s) 206, 228
BstV1I GCAGC 3 cut(s) 105, 125, 126
BstXI CCANNNNNNTGG 2 cut(s) 193, 813
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 1 cut(s) 43
BtgI CCRYGG 2 cut(s) 38, 226
BtsCI GGATG 2 cut(s) 399, 625
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 2 cut(s) 191, 780
BtuMI TCGCGA 1 cut(s) 206
Cac8I GCNNGC 6 cut(s) 65, 124, 204, 371, 584, 711
CaiI CAGNNNCTG 1 cut(s) 958
CfoI GCGC 2 cut(s) 63, 399
Cfr13I GGNCC 2 cut(s) 286, 914
Cfr42I CCGCGG 1 cut(s) 229
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 655
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 2 cut(s) 291, 424
DpnI GATC 5 cut(s) 167, 236, 279, 452, 555
DpnII GATC 5 cut(s) 165, 234, 277, 450, 553
DriI GACNNNNNGTC 2 cut(s) 187, 213
Eam1104I CTCTTC 3 cut(s) 11, 64, 141
Eam1105I GACNNNNNGTC 2 cut(s) 187, 213
EarI CTCTTC 3 cut(s) 11, 64, 141
EciI GGCGGA 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 588
Eco47I GGWCC 2 cut(s) 286, 914
Eco53kI GAGCTC 1 cut(s) 586
Eco57I CTGAAG 1 cut(s) 266
Eco72I CACGTG 2 cut(s) 265, 304
EcoICRI GAGCTC 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 588
FaqI GGGAC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 254
FbaI TGATCA 2 cut(s) 234, 450
Fnu4HI GCNGC 5 cut(s) 94, 115, 139, 508, 953
FokI GGATG 2 cut(s) 406, 632
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 5 cut(s) 94, 115, 139, 508, 953
FspAI RTGCGCAY 1 cut(s) 398
FspBI CTAG 1 cut(s) 179
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 2 cut(s) 62, 398
GluI GCNGC 5 cut(s) 94, 115, 139, 508, 953
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 1 cut(s) 43
HgaI GACGC 1 cut(s) 191
HhaI GCGC 2 cut(s) 63, 399
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 2 cut(s) 61, 397
HinP1I GCGC 2 cut(s) 61, 397
HincII GTYRAC 2 cut(s) 703, 856
HindII GTYRAC 2 cut(s) 703, 856
HinfI GANTC 5 cut(s) 349, 419, 500, 758, 942
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 2 cut(s) 703, 856
Hpy188I TCNGA 5 cut(s) 9, 234, 427, 569, 941
Hpy188III TCNNGA 2 cut(s) 179, 205
Hpy8I GTNNAC 2 cut(s) 703, 856
Hpy99I CGWCG 2 cut(s) 32, 211
HpyAV CCTTC 4 cut(s) 142, 239, 467, 872
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 5 cut(s) 153, 264, 274, 303, 330
HpyCH4V TGCA 9 cut(s) 55, 93, 407, 610, 623, 773, 845, 851, 868
HpyF3I CTNAG 2 cut(s) 291, 424
HpySE526I ACGT 5 cut(s) 153, 264, 274, 303, 330
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 2 cut(s) 61, 397
Ksp22I TGATCA 2 cut(s) 234, 450
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 5 cut(s) 165, 234, 277, 450, 553
LguI GCTCTTC 1 cut(s) 64
LmnI GCTCC 5 cut(s) 101, 122, 249, 471, 960
Lsp1109I GCAGC 3 cut(s) 105, 125, 126
LweI GCATC 7 cut(s) 42, 189, 384, 408, 560, 597, 610
MaeI CTAG 1 cut(s) 179
MaeII ACGT 5 cut(s) 153, 264, 274, 303, 330
MaeIII GTNAC 3 cut(s) 189, 299, 350
MalI GATC 5 cut(s) 167, 236, 279, 452, 555
MbiI CCGCTC 1 cut(s) 122
MboI GATC 5 cut(s) 165, 234, 277, 450, 553
MboII GAAGA 7 cut(s) 28, 81, 158, 254, 272, 753, 883
MfeI CAATTG 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 588
MluCI AATT 1 cut(s) 313
MlyI GAGTC 2 cut(s) 358, 509
MmeI TCCRAC 2 cut(s) 477, 501
MroXI GAANNNNTTC 1 cut(s) 882
MseI TTAA 2 cut(s) 644, 780
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 228
MspCI CTTAAG 1 cut(s) 643
MunI CAATTG 1 cut(s) 313
Mva1269I GAATGC 2 cut(s) 407, 431
MvnI CGCG 2 cut(s) 206, 228
NdeI CATATG 1 cut(s) 254
NdeII GATC 5 cut(s) 165, 234, 277, 450, 553
NlaIV GGNNCC 3 cut(s) 224, 288, 467
NmuCI GTSAC 3 cut(s) 189, 299, 350
NruI TCGCGA 1 cut(s) 206
NsbI TGCGCA 1 cut(s) 398
NspI RCATGY 5 cut(s) 67, 216, 364, 713, 845
PaeI GCATGC 2 cut(s) 67, 713
PciI ACATGT 2 cut(s) 212, 360
PciSI GCTCTTC 1 cut(s) 64
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 711
PctI GAATGC 2 cut(s) 407, 431
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 3 cut(s) 419, 758, 942
PkrI GCNGC 5 cut(s) 95, 116, 140, 509, 954
PleI GAGTC 2 cut(s) 357, 508
PmaCI CACGTG 2 cut(s) 265, 304
PmlI CACGTG 2 cut(s) 265, 304
PpsI GAGTC 2 cut(s) 357, 508
Ppu21I YACGTR 3 cut(s) 265, 275, 304
PpuMI RGGWCCY 1 cut(s) 286
PscI ACATGT 2 cut(s) 212, 360
Psp124BI GAGCTC 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 286
PspCI CACGTG 2 cut(s) 265, 304
PspFI CCCAGC 1 cut(s) 669
PspN4I GGNNCC 3 cut(s) 224, 288, 467
PspPI GGNCC 2 cut(s) 286, 914
PspPPI RGGWCCY 1 cut(s) 286
PstI CTGCAG 2 cut(s) 775, 870
PstNI CAGNNNCTG 1 cut(s) 958
RruI TCGCGA 1 cut(s) 206
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
RseI CAYNNNNRTG 1 cut(s) 714
SacI GAGCTC 1 cut(s) 588
SacII CCGCGG 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 644, 780
SatI GCNGC 5 cut(s) 94, 115, 139, 508, 953
Sau3AI GATC 5 cut(s) 165, 234, 277, 450, 553
Sau96I GGNCC 2 cut(s) 286, 914
SchI GAGTC 2 cut(s) 358, 509
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 7 cut(s) 42, 189, 384, 408, 560, 597, 610
SfcI CTRYAG 2 cut(s) 771, 866
Sfr303I CCGCGG 1 cut(s) 229
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 2 cut(s) 286, 914
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 4 cut(s) 643, 752, 785, 918
SmoI CTYRAG 4 cut(s) 643, 752, 785, 918
SphI GCATGC 2 cut(s) 67, 713
Sse9I AATT 1 cut(s) 313
SsiI CCGC 6 cut(s) 13, 47, 120, 226, 228, 507
SspMI CTAG 1 cut(s) 179
SstI GAGCTC 1 cut(s) 588
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 5 cut(s) 156, 267, 277, 306, 333
TaqI TCGA 2 cut(s) 387, 738
TasI AATT 1 cut(s) 313
TauI GCSGC 1 cut(s) 510
TfiI GAWTC 3 cut(s) 419, 758, 942
Tru1I TTAA 2 cut(s) 644, 780
Tru9I TTAA 2 cut(s) 644, 780
TscAI CASTG 2 cut(s) 198, 780
TseFI GTSAC 3 cut(s) 189, 299, 350
TseI GCWGC 4 cut(s) 93, 114, 138, 952
Tsp45I GTSAC 3 cut(s) 189, 299, 350
TspDTI ATGAA 1 cut(s) 758
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 2 cut(s) 198, 780
Vha464I CTTAAG 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 286, 914
XbaI TCTAGA 1 cut(s) 178
XceI RCATGY 5 cut(s) 67, 216, 364, 713, 845
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.