Rw1G025220

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014124
Physical Location & Seq
Reverse (-)
51804536 .. 51808011
3476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025220.1

Sequence Viewer

Length: 1014 bp
ATGAAATTATCAAAAAAAAGAAAAGAAAAGTTAATAACAAATAATAAAGGGGAGAGAGAGAAAATGCCGAAGCGAAAGCAGCGAACCGGAGCCGTTCAGCCTCTGAAGACGCAGAAGCGTATGCGAAGAGCCAAGCCGATTTGCTCTTGCACCTCTCCTCGTTTCACTTCTTCTGGCCCCGCATTCACCTGTCACGAGCAGGACGTTTGGACGGAGATCGCAAAGTTTCTGGACGGAAAGTCACTGGTGATGCTCGCTGCGACATGTCGTTGGTTCCACGCGCTGGTCGAGGACGGGAGCATATGGAGGTACTTGTGCTTGCGTGATCTTCAGGTCCCTGCTCCTCGCCACCCGGCGGCGTTTGATTGGAAGAAGCTCTACATTTCCGCTTTCAATGGGAGTCACTCTTACATGTTCCGCCATCCGGAGAAGCATATCGACTGGATGCGTATCGGTGCATTTTTCCTGGAATCTTCTTCAGTACTCCTAACAGAGAGGCTGAGCACCCCACTGAAATTCCCCAAGGAAGATAGCATTCAGAAAATGTTGGAGTCGAGCGGCAGTTGTGTGTTGAACAATGTGAAACCTGGGATATGGATTGCAGATCTCCAGCTTGTTCGATGCCCTGTCTGCGAGCTCAATACTTGTGATGGAACAATGCAAACATTGGATGCAAGGCATATTGAACTGTTCTTGAGTGAGGGATACCAGGATGGTAGCTGGGAATATGAACTTATTGGAATTCAGGATGTCAAAAAGCATGTTGAGGGTGCTTCTGGAGCTATTTTCGATGTCAAACACCTCAAAGACTCTTCAACTTCAGCAGTCTTCAACCTCAAGTCATGGGTCGGGAAACCGAATGACTGGCAACCGAAGGCTGTGATCACAATGCATGCAGTTGCTGTCAACACAAATCTGCAGAGAAATGAAGGCCTGCAAGTCAAATACCATGTGATGACAGCTGGAGCTGGAGGAGAAGTTGTATCTATTCGAATCTCGCAGCAGCTTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

337

Amino Acids

38.22

Weight (kDa)

9.37

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 68 - 108 1.4e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61730 AT5G36000
fragaria_vesca FvH4_7g16360 FvH4_7g16360
malus_domestica MD01G1080100.v1.1 MD07G1149100.v1.1
prunus_persica Prupe.2G187500_v2.0.a1
pyrus_communis pycom01g10840
rosa_chinensis RchiOBHm_Chr1g0359371
rosa_laevigata RLG00000027875
rosa_multiflora Rmu_co8499881.1_g000001
rosa_rugosa Rorug01G0270600
rosa_samantha Rh1AG284100 Rh1BG250200 Rh1CG268100 Rh1DG279100
rosa_wichuraiana Rw1G025220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 283
AccBSI CCGCTC 1 cut(s) 558
AccII CGCG 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 5 cut(s) 180, 356, 387, 418, 558
AcsI RAATTY 2 cut(s) 515, 741
AcuI CTGAAG 4 cut(s) 125, 314, 462, 804
AfaI GTAC 2 cut(s) 311, 483
AfiI CCNNNNNNNGG 3 cut(s) 283, 355, 424
AflIII ACRYGT 2 cut(s) 263, 411
AgsI TTSAA 5 cut(s) 394, 574, 686, 816, 832
AhdI GACNNNNNGTC 1 cut(s) 238
AjnI CCWGG 3 cut(s) 465, 586, 708
AluBI AGCT 8 cut(s) 376, 613, 637, 720, 782, 962, 968, 1006
AluI AGCT 8 cut(s) 376, 613, 637, 720, 782, 962, 968, 1006
Alw21I GWGCWC 2 cut(s) 506, 639
AlwNI CAGNNNCTG 3 cut(s) 103, 902, 1009
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 2 cut(s) 175, 931
ApeKI GCWGC 4 cut(s) 79, 257, 1000, 1003
ApoI RAATTY 2 cut(s) 515, 741
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 176, 334
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 2 cut(s) 178, 259
AsuII TTCGAA 1 cut(s) 991
AvaII GGWCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 639
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
BauI CACGAG 1 cut(s) 194
BbsI GAAGAC 2 cut(s) 113, 820
Bbv12I GWGCWC 2 cut(s) 506, 639
BbvI GCAGC 2 cut(s) 91, 244
BccI CCATC 3 cut(s) 429, 644, 707
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 3 cut(s) 467, 588, 710
BciVI GTATCC 1 cut(s) 698
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 917, 1010
BfuI GTATCC 1 cut(s) 698
BglII AGATCT 1 cut(s) 604
BisI GCNGC 6 cut(s) 80, 258, 357, 559, 1001, 1004
BlpI GCTNAGC 1 cut(s) 500
BlsI GCNGC 6 cut(s) 81, 259, 358, 560, 1002, 1005
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 4 cut(s) 353, 467, 588, 710
Bme18I GGWCC 1 cut(s) 334
BmeRI GACNNNNNGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 176, 334
BmiI GGNNCC 4 cut(s) 91, 178, 275, 336
BmrFI CCNGG 4 cut(s) 353, 467, 588, 710
BmsI GCATC 4 cut(s) 240, 435, 611, 661
BpiI GAAGAC 2 cut(s) 113, 820
BpmI CTGGAG 4 cut(s) 593, 798, 984, 990
Bpu1102I GCTNAGC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 991
BpuEI CTTGAG 2 cut(s) 715, 821
BpuMI CCSGG 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 522, 587
BsaWI WCCGGW 2 cut(s) 86, 424
BsaXI ACNNNNNCTCC 4 cut(s) 963, 966, 993, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 355, 424
Bse1I ACTGG 3 cut(s) 249, 446, 869
Bse8I GATNNNNATC 1 cut(s) 449
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 3 cut(s) 467, 588, 710
BseDI CCNNGG 2 cut(s) 522, 587
BseGI GGATG 5 cut(s) 421, 450, 676, 718, 754
BseJI GATNNNNATC 1 cut(s) 449
BseLI CCNNNNNNNGG 3 cut(s) 283, 355, 424
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 3 cut(s) 249, 446, 869
BseRI GAGGAG 3 cut(s) 147, 333, 987
BseXI GCAGC 2 cut(s) 91, 244
BseYI CCCAGC 1 cut(s) 720
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 2 cut(s) 177, 933
BsiHKAI GWGCWC 2 cut(s) 506, 639
BsiSI CCGG 3 cut(s) 87, 353, 425
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 283, 355, 424
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 2 cut(s) 182, 534
BsnI GGCC 2 cut(s) 177, 933
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 2 cut(s) 506, 639
Bsp13I TCCGGA 1 cut(s) 424
Bsp143I GATC 4 cut(s) 216, 325, 604, 882
Bsp1720I GCTNAGC 1 cut(s) 500
BspACI CCGC 5 cut(s) 180, 356, 387, 418, 558
BspANI GGCC 2 cut(s) 177, 933
BspCNI CTCAG 1 cut(s) 492
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 4 cut(s) 91, 178, 275, 336
BspMAI CTGCAG 1 cut(s) 921
BspQI GCTCTTC 1 cut(s) 121
BspT104I TTCGAA 1 cut(s) 991
BsrBI CCGCTC 1 cut(s) 558
BsrI ACTGG 3 cut(s) 249, 446, 869
BssECI CCNNGG 2 cut(s) 522, 587
BssMI GATC 4 cut(s) 216, 325, 604, 882
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 3 cut(s) 467, 588, 710
Bst4CI ACNGT 1 cut(s) 690
Bst6I CTCTTC 2 cut(s) 121, 817
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 5 cut(s) 255, 320, 635, 894, 935
BstDEI CTNAG 1 cut(s) 500
BstF5I GGATG 5 cut(s) 421, 450, 676, 718, 754
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 4 cut(s) 219, 328, 607, 885
BstMBI GATC 4 cut(s) 216, 325, 604, 882
BstMWI GCNNNNNNNGC 3 cut(s) 79, 630, 779
BstNI CCWGG 3 cut(s) 467, 588, 710
BstNSI RCATGY 4 cut(s) 267, 415, 764, 896
BstSCI CCNGG 4 cut(s) 351, 465, 586, 708
BstSFI CTRYAG 2 cut(s) 917, 1010
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 91, 244
BstV2I GAAGAC 2 cut(s) 113, 820
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 2 cut(s) 177, 933
BtsCI GGATG 5 cut(s) 421, 450, 676, 718, 754
BtsIMutI CAGTG 2 cut(s) 242, 509
Cac8I GCNNGC 5 cut(s) 255, 320, 635, 894, 935
CaiI CAGNNNCTG 3 cut(s) 103, 902, 1009
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 176, 334
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 310, 482
CviAII CATG 6 cut(s) 264, 412, 761, 843, 893, 950
CviQI GTAC 2 cut(s) 310, 482
DdeI CTNAG 1 cut(s) 500
DpnI GATC 4 cut(s) 218, 327, 606, 884
DpnII GATC 4 cut(s) 216, 325, 604, 882
DriI GACNNNNNGTC 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 121, 817
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 2 cut(s) 121, 817
EciI GGCGGA 1 cut(s) 407
Ecl136II GAGCTC 1 cut(s) 637
Eco130I CCWWGG 1 cut(s) 522
Eco147I AGGCCT 1 cut(s) 933
Eco24I GRGCYC 1 cut(s) 639
Eco47I GGWCC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 637
Eco57I CTGAAG 4 cut(s) 125, 314, 462, 804
EcoICRI GAGCTC 1 cut(s) 637
EcoO109I RGGNCCY 1 cut(s) 334
EcoRI GAATTC 1 cut(s) 741
EcoRII CCWGG 3 cut(s) 465, 586, 708
EcoT14I CCWWGG 1 cut(s) 522
EcoT22I ATGCAT 1 cut(s) 894
EcoT38I GRGCYC 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 6 cut(s) 267, 415, 764, 846, 896, 953
FaqI GGGAC 1 cut(s) 320
FatI CATG 6 cut(s) 263, 411, 760, 842, 892, 949
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 302
FbaI TGATCA 1 cut(s) 882
Fnu4HI GCNGC 6 cut(s) 80, 258, 357, 559, 1001, 1004
FokI GGATG 5 cut(s) 408, 457, 683, 725, 761
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 6 cut(s) 80, 258, 357, 559, 1001, 1004
GlaI GCGC 1 cut(s) 282
GluI GCNGC 6 cut(s) 80, 258, 357, 559, 1001, 1004
GsaI CCCAGC 1 cut(s) 724
GsuI CTGGAG 4 cut(s) 593, 798, 984, 990
HaeIII GGCC 2 cut(s) 177, 933
HapII CCGG 3 cut(s) 87, 353, 425
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 283
Hin1II CATG 6 cut(s) 267, 415, 764, 846, 896, 953
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HinfI GANTC 5 cut(s) 400, 470, 551, 809, 993
HpaII CCGG 3 cut(s) 87, 353, 425
HphI GGTGA 2 cut(s) 178, 259
Hpy166II GTNNAC 1 cut(s) 907
Hpy188I TCNGA 2 cut(s) 105, 540
Hpy188III TCNNGA 7 cut(s) 194, 230, 425, 694, 746, 777, 850
Hpy8I GTNNAC 1 cut(s) 907
HpyAV CCTTC 2 cut(s) 868, 923
HpyCH4III ACNGT 1 cut(s) 690
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 9 cut(s) 150, 458, 602, 661, 674, 892, 896, 919, 937
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 630, 779
HpyF3I CTNAG 1 cut(s) 500
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 6 cut(s) 267, 415, 764, 846, 896, 953
HspAI GCGC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 882
Kzo9I GATC 4 cut(s) 216, 325, 604, 882
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 5 cut(s) 89, 297, 346, 779, 965
Lsp1109I GCAGC 2 cut(s) 91, 244
LweI GCATC 4 cut(s) 240, 435, 611, 661
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 3 cut(s) 191, 240, 401
MalI GATC 4 cut(s) 218, 327, 606, 884
MbiI CCGCTC 1 cut(s) 558
MboI GATC 4 cut(s) 216, 325, 604, 882
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 506, 639
MluCI AATT 3 cut(s) 5, 515, 741
MlyI GAGTC 3 cut(s) 409, 560, 803
MmeI TCCRAC 1 cut(s) 528
Mph1103I ATGCAT 1 cut(s) 894
MroI TCCGGA 1 cut(s) 424
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 962
MspI CCGG 3 cut(s) 87, 353, 425
MspR9I CCNGG 4 cut(s) 353, 467, 588, 710
Mva1269I GAATGC 2 cut(s) 182, 534
MvaI CCWGG 3 cut(s) 467, 588, 710
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 79, 630, 779
NciI CCSGG 1 cut(s) 353
NdeI CATATG 1 cut(s) 302
NdeII GATC 4 cut(s) 216, 325, 604, 882
NlaIII CATG 6 cut(s) 267, 415, 764, 846, 896, 953
NlaIV GGNNCC 4 cut(s) 91, 178, 275, 336
NmuCI GTSAC 3 cut(s) 191, 240, 401
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 4 cut(s) 267, 415, 764, 896
NspV TTCGAA 1 cut(s) 991
PaeI GCATGC 1 cut(s) 896
PceI AGGCCT 1 cut(s) 933
PciI ACATGT 2 cut(s) 263, 411
PciSI GCTCTTC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 285
PctI GAATGC 2 cut(s) 182, 534
PfeI GAWTC 2 cut(s) 470, 993
PflMI CCANNNNNTGG 1 cut(s) 283
PfoI TCCNGGA 1 cut(s) 465
PkrI GCNGC 6 cut(s) 81, 259, 358, 560, 1002, 1005
PleI GAGTC 3 cut(s) 408, 559, 803
PpsI GAGTC 3 cut(s) 408, 559, 803
PpuMI RGGWCCY 1 cut(s) 334
PscI ACATGT 2 cut(s) 263, 411
Psp124BI GAGCTC 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 3 cut(s) 465, 586, 708
PspFI CCCAGC 1 cut(s) 720
PspGI CCWGG 3 cut(s) 465, 586, 708
PspN4I GGNNCC 4 cut(s) 91, 178, 275, 336
PspPI GGNCC 2 cut(s) 176, 334
PspPPI RGGWCCY 1 cut(s) 334
PstI CTGCAG 1 cut(s) 921
PstNI CAGNNNCTG 3 cut(s) 103, 902, 1009
PsuI RGATCY 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 2 cut(s) 311, 483
RsaNI GTAC 2 cut(s) 310, 482
SacI GAGCTC 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 6 cut(s) 80, 258, 357, 559, 1001, 1004
Sau3AI GATC 4 cut(s) 216, 325, 604, 882
Sau96I GGNCC 2 cut(s) 176, 334
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 3 cut(s) 409, 560, 803
ScrFI CCNGG 4 cut(s) 353, 467, 588, 710
SduI GDGCHC 2 cut(s) 506, 639
SfaNI GCATC 4 cut(s) 240, 435, 611, 661
SfcI CTRYAG 2 cut(s) 917, 1010
SfuI TTCGAA 1 cut(s) 991
SinI GGWCC 1 cut(s) 334
SmlI CTYRAG 2 cut(s) 694, 836
SmoI CTYRAG 2 cut(s) 694, 836
SphI GCATGC 1 cut(s) 896
Sse9I AATT 3 cut(s) 5, 515, 741
SseBI AGGCCT 1 cut(s) 933
SsiI CCGC 5 cut(s) 180, 356, 387, 418, 558
SstI GAGCTC 1 cut(s) 639
StuI AGGCCT 1 cut(s) 933
StyD4I CCNGG 4 cut(s) 351, 465, 586, 708
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 690
TaiI ACGT 1 cut(s) 207
TaqI TCGA 6 cut(s) 288, 438, 554, 619, 789, 991
TasI AATT 3 cut(s) 5, 515, 741
TatI WGTACW 1 cut(s) 481
TauI GCSGC 2 cut(s) 359, 561
TfiI GAWTC 2 cut(s) 470, 993
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 2 cut(s) 249, 516
TseFI GTSAC 3 cut(s) 191, 240, 401
TseI GCWGC 4 cut(s) 79, 257, 1000, 1003
Tsp45I GTSAC 3 cut(s) 191, 240, 401
TspDTI ATGAA 3 cut(s) 17, 744, 942
TspGWI ACGGA 2 cut(s) 227, 249
TspRI CASTG 2 cut(s) 249, 516
Van91I CCANNNNNTGG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 2 cut(s) 515, 741
XceI RCATGY 4 cut(s) 267, 415, 764, 896
ZrmI AGTACT 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.