MD01G1082100.v1.1

UV-stimulated scaffold protein A homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014390
Physical Location & Seq
Reverse (-)
18857130 .. 18859816
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1082100.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGGAAGAAGAGGAAGGAGGGAAGGTTAGGGTTTTGATAGAGAACGCGACGATCTCCATCGAACCGGAGGTCGACCCACGCCTCCTAAAAGCCATCAAGTCGGTGGTCCGTTACTCCGATTCGGAGCTCCGACTCGCCGCCCGTATTCTCATGGATCTTATGAAGCGCGAGCACTCTCAGGTGCGGTACCTCACACTACTTATAATTGATGAGCTTTTTATGAGGTCAAAGCTCTTTAGAGCTCTTGTTGTGGAGAATTTGGATCAATTGCTGACTTTAAGTGTCGGGTTTCGTATACGTTCGTCGCTCCCACCTCCGGAAAATGTTGCAACTGCTCTGCGTAAGAAAGCGATTGAGTTTCTGGAGAAGTGGAATTCTTCATTTGGGGTTCACTATAAGCAGATTAGATTAGGGTTTGATTACCTCAGGAACACCCTTAAGTATCAGTTCCCAAATCTACAGGAAACTGCGACTCGGCTTGAGCAGCAGAGAAGGGAAAGGGAGAGGAAGACGAAAAAGATCTTATTGAACAAGTTTAAGAATTTGGGAAATAATTTTGCATCAATTAAGGAAGAAATACAGTCGACTATTGATGAGATTGGGGAGTGTTTAGAAATTGTACGCCCCAAAGAGGAAGGTGTGCCGTTGTGTCCTTTAGATGATGAAGATATGGAGGAATTTCATTCTTATGAGTTTCGGCAATTGCGCTTACATGCATTGGAAGAAGGAGAAAAAGTTCATGAGAATAGTGAGAACAAAGTGGTTTTTGATGCCCTGCGGGAGCTTTACAAGATATTGGTAAAGCATTTGGCTGCAGTGCAAGAATGGGTCCTTGTTCTTGTAAAAGTTGAACTGACTGATAACAGGTCCAGAGATTCGTATTTGAAGGAGTTCATTGATATCCGGAATAATATTCAATCAGTGAAGAAGAAGTGTGAAGAAGCGGGATGCACTCTTTCAAACACTGCAAATCATGAGGAAGAAATTTTTTGGGAAGAAGGCAAGATTGAAACAACTGAGGGTGGGAGGTCTAGTCTTCCCAGTAACAAACCGGGAGGTCTAGCTGTGGCATCAACACCCAAGGAGGTGCAAGGTAGACTTCCTAAACGCTATGATAATCCTAAATGCCAGGATAATGAGGGCGATGGCAGTAAAAGGCTCAGTTGTGAAGGTGGGGGAAGCAATTCAGACCCTCTGAGGAGTAAGCTTCTGGCTGAAGCTCCAGAGGTGAACTGGAGCACTCATTTGGCCAGTTGGGGTTCAAAGAGGGATGTATTGGCTAACCATCGGGGCTTGGAGGTTGAAAGTCATTGGGGCAGGGTGGATCAGGATGCAGTTATTCCAGCAGAAAAGATTGCGGAGTTAAATCTTCAGGCTACTGTATACAAAGAAAAGCAGGTTGAAATTCAGCCATGCCGAGCACCATTAAGCAATGGAAAACTTTGTCCGAGAAGAGACTTGAGAATTTGCCCATTTCATGGACCTGTTATACCCAGAGATGATGAGGGAAAACCACTGAATCAGAACCCTCCAAAAGACGAGAATTATTTAGGGACCGATTTGGTAGAGCAGTTGGTGAAACAAGCCGTGCAGAATGTTCGTGAGAAAGATAAAGAGTTGTCAAGGAAGAGAGAAATAGATAAAAAGGCACTGAAGCGTGCCAGGCTTGCCAAAATACGAGAACACAATGAAGCGGTTTTACGAGATGCTGCAGTCGCTTCAACTTCTAACTCTGCATTTATTGGAGAAGATTGGGAGCCAAGTACTAGTAGGAACACATCATTCAGAAACAAAAAGAAAACCCTTGCTTCCTTGCTGCAGAAGAAAGAGACACCAAAAGATAGATTAGCTCGGAGGATTTTGAATACACGAGCAAGCGCTGCCACTGGAAGACAACTTACATTGGGCGAGGATGCATATTACCGAGAAGCCTTCCCGAATCAGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2043

Protein Analysis

650

Amino Acids

74.34

Weight (kDa)

8.48

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UVSSA_N PF20867 9 - 145 3.2e-36 UVSSA N-terminal domain
DUF2043 PF09740 406 - 508 3e-31 Uncharacterized conserved protein (DUF2043)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61800 AT3G61800
fragaria_vesca FvH4_7g16650
malus_domestica MD01G1082100.v1.1 MD07G1150500.v1.1
prunus_persica Prupe.2G189300_v2.0.a1
pyrus_communis pycom07g14900
rosa_chinensis RchiOBHm_Chr1g0359811
rosa_laevigata RLG00000027852
rosa_multiflora Rmu_ssc0000371.1_g000006
rosa_roxburghii Rroxscaffold_4G00296810
rosa_rugosa Rorug01G0272800
rosa_samantha Rh1AG286700 Rh1BG252600 Rh1CG269800 Rh1DG281600
rosa_wichuraiana Rw1G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 1387
Acc65I GGTACC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 1478
AccI GTMKAC 5 cut(s) 72, 295, 584, 1096, 1383
AccII CGCG 2 cut(s) 47, 168
AccIII TCCGGA 2 cut(s) 316, 903
AciI CCGC 6 cut(s) 138, 184, 778, 944, 1358, 1694
AclWI GGATC 3 cut(s) 162, 270, 1332
AcoI YGGCCR 1 cut(s) 1248
AcsI RAATTY 7 cut(s) 256, 373, 541, 677, 984, 1404, 1464
AcuI CTGAAG 3 cut(s) 1236, 1355, 1673
AfaI GTAC 3 cut(s) 188, 621, 1765
AfeI AGCGCT 1 cut(s) 1879
AfiI CCNNNNNNNGG 3 cut(s) 316, 631, 1478
AflII CTTAAG 1 cut(s) 437
AhlI ACTAGT 1 cut(s) 1766
AjnI CCWGG 2 cut(s) 1128, 1661
AloI GAACNNNNNNTCC 2 cut(s) 720, 752
AluBI AGCT 9 cut(s) 127, 214, 232, 242, 784, 1064, 1207, 1220, 1850
AluI AGCT 9 cut(s) 127, 214, 232, 242, 784, 1064, 1207, 1220, 1850
Alw21I GWGCWC 5 cut(s) 129, 174, 244, 1241, 1423
Alw26I GTCTC 2 cut(s) 1449, 1823
AlwI GGATC 3 cut(s) 162, 270, 1332
Aor13HI TCCGGA 2 cut(s) 316, 903
Aor51HI AGCGCT 1 cut(s) 1879
AoxI GGCC 1 cut(s) 1248
ApeKI GCWGC 5 cut(s) 484, 812, 1709, 1816, 1880
ApoI RAATTY 7 cut(s) 256, 373, 541, 677, 984, 1404, 1464
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
Asp700I GAANNNNTTC 3 cut(s) 735, 890, 1183
Asp718I GGTACC 1 cut(s) 186
AspLEI GCGC 3 cut(s) 168, 708, 1880
AspS9I GGNCC 5 cut(s) 106, 829, 867, 1481, 1554
AsuC2I CCSGG 1 cut(s) 1053
AsuHPI GGTGA 2 cut(s) 1240, 1588
AvaII GGWCC 5 cut(s) 106, 829, 867, 1481, 1554
AxyI CCTNAGG 1 cut(s) 425
BalI TGGCCA 1 cut(s) 1250
BanI GGYRCC 1 cut(s) 186
BanII GRGCYC 2 cut(s) 129, 244
BarI GAAGNNNNNNTAC 4 cut(s) 1683, 1715, 1882, 1914
BauI CACGAG 1 cut(s) 1869
BbsI GAAGAC 3 cut(s) 515, 1028, 1897
Bbv12I GWGCWC 5 cut(s) 129, 174, 244, 1241, 1423
BbvI GCAGC 5 cut(s) 496, 799, 1696, 1803, 1867
BccI CCATC 4 cut(s) 65, 101, 1139, 1293
BceAI ACGGC 2 cut(s) 628, 1571
BcgI CGANNNNNNTGC 2 cut(s) 1580, 1614
BciT130I CCWGG 2 cut(s) 1130, 1663
BcnI CCSGG 1 cut(s) 1053
BcoDI GTCTC 2 cut(s) 1449, 1823
BcuI ACTAGT 1 cut(s) 1766
BfaI CTAG 3 cut(s) 1032, 1061, 1767
BfmI CTRYAG 4 cut(s) 458, 813, 1710, 1817
BfoI RGCGCY 1 cut(s) 1881
BfrI CTTAAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 1387
BglII AGATCT 1 cut(s) 519
BisI GCNGC 6 cut(s) 138, 485, 813, 1710, 1817, 1881
BlsI GCNGC 6 cut(s) 139, 486, 814, 1711, 1818, 1882
BmcAI AGTACT 1 cut(s) 1765
Bme1390I CCNGG 3 cut(s) 1053, 1130, 1663
Bme18I GGWCC 5 cut(s) 106, 829, 867, 1481, 1554
BmgT120I GGNCC 5 cut(s) 106, 829, 867, 1481, 1554
BmiI GGNNCC 4 cut(s) 188, 830, 1555, 1758
BmrFI CCNGG 3 cut(s) 1053, 1130, 1663
BmrI ACTGGG 1 cut(s) 1035
BmsI GCATC 7 cut(s) 569, 760, 938, 1079, 1321, 1696, 1903
BmuI ACTGGG 1 cut(s) 1035
BpiI GAAGAC 3 cut(s) 515, 1028, 1897
BpmI CTGGAG 3 cut(s) 383, 1206, 1255
BpuEI CTTGAG 2 cut(s) 500, 1480
BpuMI CCSGG 1 cut(s) 1053
BsaBI GATNNNNATC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 1080
BsaWI WCCGGW 3 cut(s) 64, 316, 903
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 720, 750, 1018, 1048
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 631, 1478
Bse1I ACTGG 4 cut(s) 1041, 1238, 1251, 1891
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 1438
Bse8I GATNNNNATC 1 cut(s) 56
BseAI TCCGGA 2 cut(s) 316, 903
BseBI CCWGG 2 cut(s) 1130, 1663
BseDI CCNNGG 1 cut(s) 1080
BseGI GGATG 4 cut(s) 953, 1276, 1336, 1918
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 3 cut(s) 316, 631, 1478
BseMI GCAATG 1 cut(s) 1438
BseMII CTCAG 5 cut(s) 191, 439, 1008, 1174, 1187
BseNI ACTGG 4 cut(s) 1041, 1238, 1251, 1891
BseRI GAGGAG 1 cut(s) 1213
BseXI GCAGC 5 cut(s) 496, 799, 1696, 1803, 1867
BsgI GTGCAG 1 cut(s) 1610
Bsh1236I CGCG 2 cut(s) 47, 168
BshFI GGCC 1 cut(s) 1250
BshNI GGYRCC 1 cut(s) 186
BsiHKAI GWGCWC 5 cut(s) 129, 174, 244, 1241, 1423
BsiSI CCGG 4 cut(s) 65, 317, 904, 1052
BslFI GGGAC 1 cut(s) 1567
BslI CCNNNNNNNGG 3 cut(s) 316, 631, 1478
BsmAI GTCTC 2 cut(s) 1449, 1823
BsmFI GGGAC 1 cut(s) 1567
BsnI GGCC 1 cut(s) 1250
Bsp1286I GDGCHC 5 cut(s) 129, 174, 244, 1241, 1423
Bsp13I TCCGGA 2 cut(s) 316, 903
Bsp143I GATC 5 cut(s) 51, 154, 262, 519, 1324
BspACI CCGC 6 cut(s) 138, 184, 778, 944, 1358, 1694
BspANI GGCC 1 cut(s) 1250
BspCNI CTCAG 5 cut(s) 190, 438, 1009, 1173, 1188
BspEI TCCGGA 2 cut(s) 316, 903
BspFNI CGCG 2 cut(s) 47, 168
BspHI TCATGA 2 cut(s) 739, 973
BspLI GGNNCC 4 cut(s) 188, 830, 1555, 1758
BspMAI CTGCAG 3 cut(s) 817, 1714, 1821
BspMI ACCTGC 1 cut(s) 1387
BspPI GGATC 3 cut(s) 162, 270, 1332
BspT107I GGYRCC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 1438
BsrI ACTGG 4 cut(s) 1041, 1238, 1251, 1891
BssECI CCNNGG 1 cut(s) 1080
BssMI GATC 5 cut(s) 51, 154, 262, 519, 1324
BssNAI GTATAC 2 cut(s) 296, 1384
BssSI CACGAG 1 cut(s) 1869
BssT1I CCWWGG 1 cut(s) 1080
Bst1107I GTATAC 2 cut(s) 296, 1384
Bst2BI CACGAG 1 cut(s) 1869
Bst2UI CCWGG 2 cut(s) 1130, 1663
Bst4CI ACNGT 2 cut(s) 582, 1381
Bst6I CTCTTC 3 cut(s) 3, 1447, 1622
BstAFI CTTAAG 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 1880
BstC8I GCNNGC 4 cut(s) 170, 1659, 1668, 1876
BstDEI CTNAG 5 cut(s) 177, 425, 1017, 1160, 1196
BstF5I GGATG 4 cut(s) 953, 1276, 1336, 1918
BstFNI CGCG 2 cut(s) 47, 168
BstH2I RGCGCY 1 cut(s) 1881
BstHHI GCGC 3 cut(s) 168, 708, 1880
BstKTI GATC 5 cut(s) 54, 157, 265, 522, 1327
BstMAI GTCTC 2 cut(s) 1449, 1823
BstMBI GATC 5 cut(s) 51, 154, 262, 519, 1324
BstMWI GCNNNNNNNGC 5 cut(s) 484, 1663, 1667, 1715, 1880
BstNI CCWGG 2 cut(s) 1130, 1663
BstNSI RCATGY 1 cut(s) 716
BstSCI CCNGG 3 cut(s) 1051, 1128, 1661
BstSFI CTRYAG 4 cut(s) 458, 813, 1710, 1817
BstUI CGCG 2 cut(s) 47, 168
BstV1I GCAGC 5 cut(s) 496, 799, 1696, 1803, 1867
BstV2I GAAGAC 3 cut(s) 515, 1028, 1897
BstX2I RGATCY 2 cut(s) 154, 519
BstYI RGATCY 2 cut(s) 154, 519
BstZ17I GTATAC 2 cut(s) 296, 1384
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 1 cut(s) 1250
BtgZI GCGATG 1 cut(s) 1158
BtsCI GGATG 4 cut(s) 953, 1276, 1336, 1918
BtsI GCAGTG 2 cut(s) 822, 963
BtsIMutI CAGTG 7 cut(s) 822, 927, 963, 1514, 1649, 1884, 1949
BveI ACCTGC 1 cut(s) 1387
Cac8I GCNNGC 4 cut(s) 170, 1659, 1668, 1876
CciI TCATGA 2 cut(s) 739, 973
CfoI GCGC 3 cut(s) 168, 708, 1880
Cfr13I GGNCC 5 cut(s) 106, 829, 867, 1481, 1554
Csp6I GTAC 3 cut(s) 187, 620, 1764
CviAII CATG 6 cut(s) 151, 713, 740, 974, 1413, 1478
CviQI GTAC 3 cut(s) 187, 620, 1764
DdeI CTNAG 5 cut(s) 177, 425, 1017, 1160, 1196
DpnI GATC 5 cut(s) 53, 156, 264, 521, 1326
DpnII GATC 5 cut(s) 51, 154, 262, 519, 1324
EaeI YGGCCR 1 cut(s) 1248
Eam1104I CTCTTC 3 cut(s) 3, 1447, 1622
EarI CTCTTC 3 cut(s) 3, 1447, 1622
Ecl136II GAGCTC 2 cut(s) 127, 242
Eco130I CCWWGG 1 cut(s) 1080
Eco24I GRGCYC 2 cut(s) 129, 244
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 5 cut(s) 106, 829, 867, 1481, 1554
Eco47III AGCGCT 1 cut(s) 1879
Eco53kI GAGCTC 2 cut(s) 127, 242
Eco57I CTGAAG 3 cut(s) 1236, 1355, 1673
Eco81I CCTNAGG 1 cut(s) 425
EcoICRI GAGCTC 2 cut(s) 127, 242
EcoO109I RGGNCCY 1 cut(s) 829
EcoRI GAATTC 1 cut(s) 373
EcoRII CCWGG 2 cut(s) 1128, 1661
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 1 cut(s) 1080
EcoT22I ATGCAT 2 cut(s) 718, 1918
EcoT38I GRGCYC 2 cut(s) 129, 244
ErhI CCWWGG 1 cut(s) 1080
FaeI CATG 6 cut(s) 154, 716, 743, 977, 1416, 1481
FalI AAGNNNNNCTT 2 cut(s) 1083, 1115
FaqI GGGAC 1 cut(s) 1567
FatI CATG 6 cut(s) 150, 712, 739, 973, 1412, 1477
FauI CCCGC 2 cut(s) 771, 937
FblI GTMKAC 5 cut(s) 72, 295, 584, 1096, 1383
Fnu4HI GCNGC 6 cut(s) 138, 485, 813, 1710, 1817, 1881
FokI GGATG 4 cut(s) 960, 1283, 1343, 1925
FriOI GRGCYC 2 cut(s) 129, 244
Fsp4HI GCNGC 6 cut(s) 138, 485, 813, 1710, 1817, 1881
FspBI CTAG 3 cut(s) 1032, 1061, 1767
GlaI GCGC 3 cut(s) 167, 707, 1879
GluI GCNGC 6 cut(s) 138, 485, 813, 1710, 1817, 1881
GsuI CTGGAG 3 cut(s) 383, 1206, 1255
HaeII RGCGCY 1 cut(s) 1881
HaeIII GGCC 1 cut(s) 1250
HapII CCGG 4 cut(s) 65, 317, 904, 1052
HhaI GCGC 3 cut(s) 168, 708, 1880
Hin1II CATG 6 cut(s) 154, 716, 743, 977, 1416, 1481
Hin6I GCGC 3 cut(s) 166, 706, 1878
HinP1I GCGC 3 cut(s) 166, 706, 1878
HincII GTYRAC 2 cut(s) 73, 585
HindII GTYRAC 2 cut(s) 73, 585
HindIII AAGCTT 1 cut(s) 1205
HinfI GANTC 6 cut(s) 119, 132, 472, 875, 1519, 1939
HpaII CCGG 4 cut(s) 65, 317, 904, 1052
HphI GGTGA 2 cut(s) 1240, 1588
Hpy166II GTNNAC 7 cut(s) 73, 296, 391, 585, 1097, 1231, 1384
Hpy188I TCNGA 9 cut(s) 118, 124, 131, 1189, 1197, 1449, 1524, 1787, 1854
Hpy8I GTNNAC 7 cut(s) 73, 296, 391, 585, 1097, 1231, 1384
Hpy99I CGWCG 2 cut(s) 52, 307
HpyAV CCTTC 9 cut(s) 8, 16, 486, 629, 719, 880, 992, 1163, 1942
HpyCH4III ACNGT 2 cut(s) 582, 1381
HpyCH4IV ACGT 1 cut(s) 298
HpyF10VI GCNNNNNNNGC 5 cut(s) 484, 1663, 1667, 1715, 1880
HpyF3I CTNAG 5 cut(s) 177, 425, 1017, 1160, 1196
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 6 cut(s) 154, 716, 743, 977, 1416, 1481
HspAI GCGC 3 cut(s) 166, 706, 1878
Kpn2I TCCGGA 2 cut(s) 316, 903
KpnI GGTACC 1 cut(s) 190
Kzo9I GATC 5 cut(s) 51, 154, 262, 519, 1324
LmnI GCTCC 7 cut(s) 124, 132, 312, 781, 1225, 1236, 1756
Lsp1109I GCAGC 5 cut(s) 496, 799, 1696, 1803, 1867
LweI GCATC 7 cut(s) 569, 760, 938, 1079, 1321, 1696, 1903
MaeI CTAG 3 cut(s) 1032, 1061, 1767
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 2 cut(s) 110, 1043
MalI GATC 5 cut(s) 53, 156, 264, 521, 1326
MboI GATC 5 cut(s) 51, 154, 262, 519, 1324
MfeI CAATTG 2 cut(s) 266, 701
MflI RGATCY 2 cut(s) 154, 519
MhlI GDGCHC 5 cut(s) 129, 174, 244, 1241, 1423
MlsI TGGCCA 1 cut(s) 1250
MluNI TGGCCA 1 cut(s) 1250
MlyI GAGTC 2 cut(s) 126, 466
MmeI TCCRAC 1 cut(s) 154
Mox20I TGGCCA 1 cut(s) 1250
Mph1103I ATGCAT 2 cut(s) 718, 1918
MroI TCCGGA 2 cut(s) 316, 903
MroXI GAANNNNTTC 3 cut(s) 735, 890, 1183
MscI TGGCCA 1 cut(s) 1250
MseI TTAA 6 cut(s) 278, 438, 537, 567, 1364, 1427
MslI CAYNNNNRTG 1 cut(s) 687
Msp20I TGGCCA 1 cut(s) 1250
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 4 cut(s) 65, 317, 904, 1052
MspR9I CCNGG 3 cut(s) 1053, 1130, 1663
MunI CAATTG 2 cut(s) 266, 701
MvaI CCWGG 2 cut(s) 1130, 1663
MvnI CGCG 2 cut(s) 47, 168
MwoI GCNNNNNNNGC 5 cut(s) 484, 1663, 1667, 1715, 1880
NciI CCSGG 1 cut(s) 1053
NdeII GATC 5 cut(s) 51, 154, 262, 519, 1324
NlaIII CATG 6 cut(s) 154, 716, 743, 977, 1416, 1481
NlaIV GGNNCC 4 cut(s) 188, 830, 1555, 1758
NmeAIII GCCGAG 2 cut(s) 454, 1442
NsiI ATGCAT 2 cut(s) 718, 1918
NspI RCATGY 1 cut(s) 716
PagI TCATGA 2 cut(s) 739, 973
PdmI GAANNNNTTC 3 cut(s) 735, 890, 1183
PfeI GAWTC 4 cut(s) 119, 875, 1519, 1939
PflMI CCANNNNNTGG 1 cut(s) 1478
PkrI GCNGC 6 cut(s) 139, 486, 814, 1711, 1818, 1882
PleI GAGTC 2 cut(s) 126, 466
PpsI GAGTC 2 cut(s) 126, 466
PpuMI RGGWCCY 1 cut(s) 829
PsiI TTATAA 1 cut(s) 203
Psp124BI GAGCTC 2 cut(s) 129, 244
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 2 cut(s) 1128, 1661
PspGI CCWGG 2 cut(s) 1128, 1661
PspN4I GGNNCC 4 cut(s) 188, 830, 1555, 1758
PspPI GGNCC 5 cut(s) 106, 829, 867, 1481, 1554
PspPPI RGGWCCY 1 cut(s) 829
PstI CTGCAG 3 cut(s) 817, 1714, 1821
PsuI RGATCY 2 cut(s) 154, 519
RsaI GTAC 3 cut(s) 188, 621, 1765
RsaNI GTAC 3 cut(s) 187, 620, 1764
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 2 cut(s) 129, 244
SalI GTCGAC 2 cut(s) 71, 583
SaqAI TTAA 6 cut(s) 278, 438, 537, 567, 1364, 1427
SatI GCNGC 6 cut(s) 138, 485, 813, 1710, 1817, 1881
Sau3AI GATC 5 cut(s) 51, 154, 262, 519, 1324
Sau96I GGNCC 5 cut(s) 106, 829, 867, 1481, 1554
ScaI AGTACT 1 cut(s) 1765
SchI GAGTC 2 cut(s) 126, 466
ScrFI CCNGG 3 cut(s) 1053, 1130, 1663
SduI GDGCHC 5 cut(s) 129, 174, 244, 1241, 1423
SfaNI GCATC 7 cut(s) 569, 760, 938, 1079, 1321, 1696, 1903
SfcI CTRYAG 4 cut(s) 458, 813, 1710, 1817
SinI GGWCC 5 cut(s) 106, 829, 867, 1481, 1554
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 3 cut(s) 437, 479, 1459
SmoI CTYRAG 3 cut(s) 437, 479, 1459
SpeI ACTAGT 1 cut(s) 1766
SsiI CCGC 6 cut(s) 138, 184, 778, 944, 1358, 1694
SspI AATATT 1 cut(s) 913
SspMI CTAG 3 cut(s) 1032, 1061, 1767
SstI GAGCTC 2 cut(s) 129, 244
StyD4I CCNGG 3 cut(s) 1051, 1128, 1661
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 2 cut(s) 582, 1381
TaiI ACGT 1 cut(s) 301
TaqI TCGA 3 cut(s) 60, 72, 584
TaqII GACCGA 1 cut(s) 1571
TatI WGTACW 1 cut(s) 1763
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 4 cut(s) 119, 875, 1519, 1939
Tru1I TTAA 6 cut(s) 278, 438, 537, 567, 1364, 1427
Tru9I TTAA 6 cut(s) 278, 438, 537, 567, 1364, 1427
TscAI CASTG 7 cut(s) 822, 927, 970, 1521, 1656, 1891, 1949
TseI GCWGC 5 cut(s) 484, 812, 1709, 1816, 1880
TspDTI ATGAA 8 cut(s) 176, 369, 671, 678, 728, 883, 1466, 1704
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 7 cut(s) 822, 927, 970, 1521, 1656, 1891, 1949
Van91I CCANNNNNTGG 1 cut(s) 1478
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 5 cut(s) 106, 829, 867, 1481, 1554
XapI RAATTY 7 cut(s) 256, 373, 541, 677, 984, 1404, 1464
XceI RCATGY 1 cut(s) 716
XcmI CCANNNNNNNNNTGG 2 cut(s) 100, 1230
XmiI GTMKAC 5 cut(s) 72, 295, 584, 1096, 1383
XmnI GAANNNNTTC 3 cut(s) 735, 890, 1183
XspI CTAG 3 cut(s) 1032, 1061, 1767
ZrmI AGTACT 1 cut(s) 1765
Zsp2I ATGCAT 2 cut(s) 718, 1918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.