MD01G1082300.v1.1
ERF Family

A domain family that is part of the cupin metalloenzyme superfamily.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002674
Physical Location & Seq
Reverse (-)
18879413 .. 18889690
10278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1082300.v1.1.491

Sequence Viewer

Length: 3300 bp
ATGGAACCGCCGGGCCGCTGCAAGCGCAACGACGGCCAGAAGTGGCGGTGCAGCGAGACGGCGGCGGAGGGGAAGTCCTATTGTCAAAAGCACTACGATCAGAAGAGAAACAATGCTATGAATAAGAAAACCAAGACGGGAAAGTCAGATGGAAACAGCGGTTCCGATAATCTAACGAAGAATCGTCGATCCAAAGAGGACAAATCTGGCAAAAAATCGGAGCAGCGTGCTTGCTCGAAGTTGCAGGAAAAACCTAGTACACAATCTGAGGAGCGTGCGAGCTCGAAGCTGCAGGATAAGTCATGCAAGAAATCGGAGCAGCGTGCGAGCTTGAAGCTGCAGGAAAAACCTAGCAAACTATCGGAGGAGCGTTCAAGCTCGAAGATGCAGGATCAGTCTAGCAAGAAATCGGAGCAGCGCGTATGCTTGAAGTTGCAGGAAAAATCCAGCAGAAAATCGGATCGGCGCGTGGGCTTGAAGTTGCAAAAAAATCCTGGCAAACAATCTGAGGAGCACGTGGGCTTGAAGGTGCCGGAAAAGTCTAGCAAAAAATCTGAGGAGCGGGAGAGCTTGAAGTTGCAGGAAAAGCCTGGCAAAAATTCGGAGCAGCGCGTGTCCTTGATGTGCCACCAGTGTCAGCGGAACGACAAGAGTGGCGTTGTCTTCTGTTCTAATTGTACAAAGCGATTTTGCTTTGAGTGCATTGAAAGATGGTACCCGGGGAAGACGAGAGAGGAAATTGGGAAGAAATGCCCGTTTTGTTGTCACAACTGCAACTGCAAAGCTTGCTTGCGTGCGTTTATTGTGAAACCTCACACTAGAAAAGTTGAACCCAGTGTCAAATTACAGCGGCTCAAGTACTTGCTCTATAAAACTTTGCCTGTTCTGAGGTATATTCATAAGGAGCAGAGCTCTGAACTAGAGATTGAGTCCAAAATACGAGGTGTTCAACTGACTGAAGGGGGGATACAAAGAACTAAAATAGACCCAAAAGAACGTATTTACTGCGACAATTGCTCTACATCTATTGTTGATTTCCACAGAAGCTGCACCAACCCCGATTGTTCCTATGATCTCTGTCTCACTTGTTGCAAAGAGCTAAGGGAAGGTTGTCAACCTGGAGGTTGTGAAGCAGAAACATCTCACCAACAATCTGTAGAAAGAGCTCATAAACAAGTGAAAAATAGAGGCAATACTAACATGAAAAGGAAAAGACATGTGTGGGAGGGCCAGATTACCTCTGCTTCTGATAATTCTAAAGCTGATGTGCCTTTTTCTCTTCCTAATTGGAAAGCGAATCCTGATGACAGCATTCCTTGTCCTCCAAAAGAGTGTGGAGGTTGTGGCCAGGTAAATCTGGAACTGAGGCGCAAATGTAAAGCTAATTGGGTAATGCAACTGTTAAAAAATGCAGAGGATTTTACTATTGACTTCAAGATGCAAGACACCGATGTTTCTCAAGGATGCTCCTTGTGCCAGCCAAATGATTCCAAGGAACATAATAATATACAGTCTGAAGTGAGGCAGGCGGCTTTCAGGGAAAACAGTGATGATAACTTCTTATACTGTCCGAATGCTATTGACATTGCAGATGATGAAATTGAACATTTTCAGAGGCACTGGATAAATGGGGAACCTGTAATAGTCAGAAATGTTCTTGACAAGACATCTGGTCTTAGTTGGGAACCTATGGTCATGTGGAGGGCTTTTAGAGAAACGGGGGCCAATGTAAAGTTTAAAGAGGAGACACGAAGTGTGAAGGCCCTTGACTGCTTTGATTGGTGTGAGGTGGAAATCAATATTCACCAGTTTTTCATGGGCTACTTGGAGGGTCGTCTACATAGAAGTGGGTGGCCTGAGATGTTGAAATTGAAGGATTGGCCTTCATCAACTTTATTTGAAGAGCGTTTGCCAAGGCATTGCACTGAATTTATTGCAGCACTCCCTTTCAGTGACTATACTGATCCAAAATATCCAGAAGTGGGTTGTCTAAATCTTGCTACAAAACTTCCTGAAGATTCATTGAAACCTGATATGGGACCCAAAACATACATTGCCTATGGATTCTCTGAAGAACTTGGCAGAGGTGATTCAGTGACAAAGCTACACTGTGATATGTCTGATGCGGTAAATGTTCTGACTCACACAACAAGAGTGAAGATCGCTCCTTGGCAGCACAAAAAGATAAAGGCTTTGCAGAGGAAGCATGAAGCTGAAGACTTGCGTGAACTGTACAATGAAAGAGATGAGGATAAGGGGACAGTTGGAGGAAAATCATCAAACAAATCTTCCGAAATCCAGATCATGGGTGCTGAATGTACTGAAAATGAGAACATTGTGGATAATGATCATCTAATGCAGAAAGATGGGAAGAAAAGAAAAATGGTTGACGAACAATCATTTAGGATTTCTCAGTTGTCAGATTCCAAGTATTTAGATGATACTGTTGAATATGATAAAACTGAGCATGTTCCAGAACCTCCTGATACTTCTGAAATACATAAATTAAAGTTTCCAACTCTTGACCATCAGAGGAATGAATTGATAAAAACCACCTTGCCTCATGCAAATGGAGTTGAAGCAGAGCAAGAACATGCTCAATGTTCCACGGACATGATGATTGAGAGATCTGGTGGGAAAGATACTTCTGTGGCTTTGTTTCCAGGAAATGATGCGGTTCATGATATGAACGTGAAAGAGTCAAACCTGACAAAAACAAAGGATTCTCTTGAAAGTAATGACAGTTTGGATGTTGCCCAAGGTGGTGCTGTTTGGGACATCTTTCGCCGCCAGGATGTCCCCAAGTTAAGGGAATACTTGGAAAAACAAAAGAAAGAATTTCGCCACATTAATAATCATCCTGTAGATTCAGTGGTTCATCCTATTCATGATCAGACCCTTTACTTGAATGAGAGACATAAGAAACAACTAAAGGAGGAATTCAATGTGGAGCCTTGGACATTTAAACAATACCTTGGTGAGGCAGTTTTTATTCCCACAGGATGTCCTCATCAAGTTAGAAATACACAATCTTGTATAAAGGTAGCACTTGACTTTGTATCTCCTGAAAGTCTTGAAGAATGTCTTCGACTGACTGAAGAGTTTCGTTTGCTCCCAAAAAACCATAGAGCCAAGGAAGACAAATTGGAGGTGAAAAAGATGACACTCTATGCTGTAAGTTCAGCTGTAAGAGAAACCAAAAAACTGATGTCAGGGCTCAAGGTGGAGCTGCACACAAAGAACCACGTGAAGGTGGGTGTTGGCTTGACATACATTGTTGACTCATTCCTAATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048522 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC WRC zf-4CXXC_R1

Protein Analysis

1100

Amino Acids

126.12

Weight (kDa)

8.51

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 4 - 43 6.9e-15 WRC
JmjC PF02373 922 - 1019 2.1e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
Acc65I GGTACC 1 cut(s) 714
AccB1I GGYRCC 2 cut(s) 529, 714
AccB7I CCANNNNNTGG 1 cut(s) 2305
AccBSI CCGCTC 1 cut(s) 562
AccI GTMKAC 1 cut(s) 1837
AccII CGCG 3 cut(s) 420, 468, 612
AclWI GGATC 4 cut(s) 183, 399, 468, 1958
AcoI YGGCCR 2 cut(s) 34, 1345
AcsI RAATTY 4 cut(s) 598, 1928, 2837, 2939
AcuI CTGAAG 6 cut(s) 978, 1536, 2034, 2091, 2235, 3117
AcvI CACGTG 2 cut(s) 517, 3247
AdeI CACNNNGTG 1 cut(s) 1754
AfaI GTAC 6 cut(s) 259, 679, 716, 860, 2234, 2320
AfiI CCNNNNNNNGG 6 cut(s) 1982, 2036, 2305, 2808, 2980, 3250
AflIII ACRYGT 1 cut(s) 1216
AhdI GACNNNNNGTC 1 cut(s) 1671
AjnI CCWGG 6 cut(s) 493, 589, 1117, 1347, 2662, 2790
Alw21I GWGCWC 4 cut(s) 284, 516, 914, 1168
Alw26I GTCTC 4 cut(s) 50, 1085, 1739, 2908
AlwI GGATC 4 cut(s) 183, 399, 468, 1958
AlwNI CAGNNNCTG 1 cut(s) 1047
Ama87I CYCGRG 1 cut(s) 718
AoxI GGCC 8 cut(s) 13, 34, 1228, 1345, 1722, 1761, 1853, 1880
ApoI RAATTY 4 cut(s) 598, 1928, 2837, 2939
AseI ATTAAT 1 cut(s) 2850
Asp700I GAANNNNTTC 3 cut(s) 1608, 3084, 3102
Asp718I GGTACC 1 cut(s) 714
AspLEI GCGC 5 cut(s) 27, 420, 468, 612, 1371
AspS9I GGNCC 5 cut(s) 13, 1228, 1722, 1762, 2039
AsuC2I CCSGG 3 cut(s) 12, 719, 720
AsuHPI GGTGA 5 cut(s) 1136, 1796, 2099, 2990, 3163
AvaI CYCGRG 1 cut(s) 718
AvaII GGWCC 1 cut(s) 2039
BaeI ACNNNNGTAYC 2 cut(s) 2634, 2667
BalI TGGCCA 1 cut(s) 1347
BanI GGYRCC 2 cut(s) 529, 714
BanII GRGCYC 4 cut(s) 284, 914, 1168, 3219
BarI GAAGNNNNNNTAC 2 cut(s) 951, 983
BbrPI CACGTG 2 cut(s) 517, 3247
BbsI GAAGAC 5 cut(s) 655, 731, 2223, 3077, 3144
Bbv12I GWGCWC 4 cut(s) 284, 516, 914, 1168
BccI CCATC 4 cut(s) 143, 705, 2360, 2535
BceAI ACGGC 2 cut(s) 49, 75
BciT130I CCWGG 6 cut(s) 495, 591, 1119, 1349, 2664, 2792
BciVI GTATCC 1 cut(s) 960
BclI TGATCA 2 cut(s) 2347, 2890
BcnI CCSGG 3 cut(s) 12, 719, 720
BcoDI GTCTC 4 cut(s) 50, 1085, 1739, 2908
BfaI CTAG 6 cut(s) 255, 351, 399, 543, 819, 920
BfmI CTRYAG 4 cut(s) 290, 338, 1155, 2862
BfuI GTATCC 1 cut(s) 960
BglII AGATCT 1 cut(s) 2627
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 9 cut(s) 12, 495, 591, 719, 720, 1119, 1349, 2664, 2792
Bme18I GGWCC 1 cut(s) 2039
BmeRI GACNNNNNGTC 1 cut(s) 1671
BmeT110I CYCGRG 1 cut(s) 718
BmgT120I GGNCC 5 cut(s) 13, 1228, 1722, 1762, 2039
BmrFI CCNGG 9 cut(s) 12, 495, 591, 719, 720, 1119, 1349, 2664, 2792
BmrI ACTGGG 1 cut(s) 828
BmsI GCATC 5 cut(s) 375, 1428, 1454, 2113, 2662
BmuI ACTGGG 1 cut(s) 828
BpiI GAAGAC 5 cut(s) 655, 731, 2223, 3077, 3144
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 1 cut(s) 1140
Bpu10I CCTNAGC 1 cut(s) 1100
BpuEI CTTGAG 3 cut(s) 839, 1443, 3203
BpuMI CCSGG 3 cut(s) 12, 719, 720
BsaAI YACGTR 2 cut(s) 517, 3247
BsaBI GATNNNNATC 1 cut(s) 2346
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 6 cut(s) 1982, 2036, 2305, 2808, 2980, 3250
Bse1I ACTGG 4 cut(s) 631, 834, 1625, 1807
Bse3DI GCAATG 3 cut(s) 1584, 1918, 2052
Bse8I GATNNNNATC 1 cut(s) 2346
BseBI CCWGG 6 cut(s) 495, 591, 1119, 1349, 2664, 2792
BseGI GGATG 6 cut(s) 1469, 2755, 2800, 2857, 2878, 3008
BseJI GATNNNNATC 1 cut(s) 2346
BseLI CCNNNNNNNGG 6 cut(s) 1982, 2036, 2305, 2808, 2980, 3250
BseMI GCAATG 3 cut(s) 1584, 1918, 2052
BseMII CTCAG 8 cut(s) 258, 498, 546, 878, 1355, 1848, 2426, 2454
BseNI ACTGG 4 cut(s) 631, 834, 1625, 1807
BseRI GAGGAG 5 cut(s) 284, 380, 524, 572, 1757
BsgI GTGCAG 3 cut(s) 70, 1033, 3215
Bsh1236I CGCG 3 cut(s) 420, 468, 612
BshFI GGCC 8 cut(s) 15, 36, 1230, 1347, 1724, 1763, 1855, 1882
BshNI GGYRCC 2 cut(s) 529, 714
BsiHKAI GWGCWC 4 cut(s) 284, 516, 914, 1168
BsiHKCI CYCGRG 1 cut(s) 718
BsiSI CCGG 3 cut(s) 11, 533, 719
BslFI GGGAC 4 cut(s) 2052, 2272, 2783, 2789
BslI CCNNNNNNNGG 6 cut(s) 1982, 2036, 2305, 2808, 2980, 3250
BsmAI GTCTC 4 cut(s) 50, 1085, 1739, 2908
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 4 cut(s) 2052, 2272, 2783, 2789
BsmI GAATGC 2 cut(s) 1311, 1579
BsnI GGCC 8 cut(s) 15, 36, 1230, 1347, 1724, 1763, 1855, 1882
BsoBI CYCGRG 1 cut(s) 718
Bsp1286I GDGCHC 5 cut(s) 284, 516, 914, 1168, 3219
Bsp1407I TGTACA 2 cut(s) 677, 2232
BspANI GGCC 8 cut(s) 15, 36, 1230, 1347, 1724, 1763, 1855, 1882
BspCNI CTCAG 8 cut(s) 259, 499, 547, 879, 1356, 1849, 2425, 2455
BspFNI CGCG 3 cut(s) 420, 468, 612
BspHI TCATGA 2 cut(s) 2680, 2887
BspMAI CTGCAG 2 cut(s) 294, 342
BspPI GGATC 4 cut(s) 183, 399, 468, 1958
BspQI GCTCTTC 1 cut(s) 1896
BspT107I GGYRCC 2 cut(s) 529, 714
BsrBI CCGCTC 1 cut(s) 562
BsrDI GCAATG 3 cut(s) 1584, 1918, 2052
BsrGI TGTACA 2 cut(s) 677, 2232
BsrI ACTGG 4 cut(s) 631, 834, 1625, 1807
BssT1I CCWWGG 7 cut(s) 1491, 1913, 2168, 2758, 2954, 2974, 3132
Bst2UI CCWGG 6 cut(s) 495, 591, 1119, 1349, 2664, 2792
Bst4CI ACNGT 9 cut(s) 1401, 1512, 1547, 1568, 2111, 2232, 2263, 2446, 2744
Bst6I CTCTTC 4 cut(s) 98, 1284, 1896, 3093
BstAPI GCANNNNNTGC 1 cut(s) 786
BstAUI TGTACA 2 cut(s) 677, 2232
BstBAI YACGTR 2 cut(s) 517, 3247
BstDSI CCRYGG 1 cut(s) 2607
BstENI CCTNNNNNAGG 1 cut(s) 2978
BstF5I GGATG 6 cut(s) 1469, 2755, 2800, 2857, 2878, 3008
BstFNI CGCG 3 cut(s) 420, 468, 612
BstHHI GCGC 5 cut(s) 27, 420, 468, 612, 1371
BstMAI GTCTC 4 cut(s) 50, 1085, 1739, 2908
BstMWI GCNNNNNNNGC 8 cut(s) 24, 33, 586, 699, 786, 1014, 1473, 2203
BstNI CCWGG 6 cut(s) 495, 591, 1119, 1349, 2664, 2792
BstNSI RCATGY 3 cut(s) 1220, 2471, 2597
BstSCI CCNGG 9 cut(s) 10, 493, 589, 717, 718, 1117, 1347, 2662, 2790
BstSFI CTRYAG 4 cut(s) 290, 338, 1155, 2862
BstUI CGCG 3 cut(s) 420, 468, 612
BstV2I GAAGAC 5 cut(s) 655, 731, 2223, 3077, 3144
BstX2I RGATCY 1 cut(s) 2627
BstYI RGATCY 1 cut(s) 2627
BsuI GTATCC 1 cut(s) 960
BsuRI GGCC 8 cut(s) 15, 36, 1230, 1347, 1724, 1763, 1855, 1882
BtgI CCRYGG 1 cut(s) 2607
BtsCI GGATG 6 cut(s) 1469, 2755, 2800, 2857, 2878, 3008
BtsIMutI CAGTG 9 cut(s) 638, 841, 1552, 1618, 1923, 1957, 2100, 2107, 2877
CaiI CAGNNNCTG 1 cut(s) 1047
CciI TCATGA 2 cut(s) 2680, 2887
CfoI GCGC 5 cut(s) 27, 420, 468, 612, 1371
Cfr13I GGNCC 5 cut(s) 13, 1228, 1722, 1762, 2039
Cfr9I CCCGGG 1 cut(s) 718
Csp6I GTAC 6 cut(s) 258, 678, 715, 859, 2233, 2319
CviQI GTAC 6 cut(s) 258, 678, 715, 859, 2233, 2319
DraI TTTAAA 2 cut(s) 1738, 2965
DraIII CACNNNGTG 1 cut(s) 1754
DrdI GACNNNNNNGTC 1 cut(s) 142
DriI GACNNNNNGTC 1 cut(s) 1671
DseDI GACNNNNNNGTC 1 cut(s) 142
EaeI YGGCCR 2 cut(s) 34, 1345
Eam1104I CTCTTC 4 cut(s) 98, 1284, 1896, 3093
Eam1105I GACNNNNNGTC 1 cut(s) 1671
EarI CTCTTC 4 cut(s) 98, 1284, 1896, 3093
EciI GGCGGA 1 cut(s) 80
Ecl136II GAGCTC 3 cut(s) 282, 912, 1166
Eco130I CCWWGG 7 cut(s) 1491, 1913, 2168, 2758, 2954, 2974, 3132
Eco24I GRGCYC 4 cut(s) 284, 914, 1168, 3219
Eco47I GGWCC 1 cut(s) 2039
Eco53kI GAGCTC 3 cut(s) 282, 912, 1166
Eco57I CTGAAG 6 cut(s) 978, 1536, 2034, 2091, 2235, 3117
Eco72I CACGTG 2 cut(s) 517, 3247
Eco88I CYCGRG 1 cut(s) 718
EcoICRI GAGCTC 3 cut(s) 282, 912, 1166
EcoNI CCTNNNNNAGG 1 cut(s) 2978
EcoO109I RGGNCCY 2 cut(s) 1762, 2039
EcoRI GAATTC 1 cut(s) 2939
EcoRII CCWGG 6 cut(s) 493, 589, 1117, 1347, 2662, 2790
EcoT14I CCWWGG 7 cut(s) 1491, 1913, 2168, 2758, 2954, 2974, 3132
EcoT38I GRGCYC 4 cut(s) 284, 914, 1168, 3219
ErhI CCWWGG 7 cut(s) 1491, 1913, 2168, 2758, 2954, 2974, 3132
Esp3I CGTCTC 1 cut(s) 50
FalI AAGNNNNNCTT 2 cut(s) 3069, 3101
FaqI GGGAC 4 cut(s) 2052, 2272, 2783, 2789
FauI CCCGC 1 cut(s) 555
FbaI TGATCA 2 cut(s) 2347, 2890
FblI GTMKAC 1 cut(s) 1837
FokI GGATG 6 cut(s) 1476, 2762, 2807, 2844, 2865, 3015
FriOI GRGCYC 4 cut(s) 284, 914, 1168, 3219
FspBI CTAG 6 cut(s) 255, 351, 399, 543, 819, 920
GlaI GCGC 5 cut(s) 26, 419, 467, 611, 1370
GsuI CTGGAG 1 cut(s) 1140
HaeIII GGCC 8 cut(s) 15, 36, 1230, 1347, 1724, 1763, 1855, 1882
HapII CCGG 3 cut(s) 11, 533, 719
HhaI GCGC 5 cut(s) 27, 420, 468, 612, 1371
Hin6I GCGC 5 cut(s) 25, 418, 466, 610, 1369
HinP1I GCGC 5 cut(s) 25, 418, 466, 610, 1369
HincII GTYRAC 3 cut(s) 1115, 2389, 3280
HindII GTYRAC 3 cut(s) 1115, 2389, 3280
HindIII AAGCTT 1 cut(s) 783
HpaII CCGG 3 cut(s) 11, 533, 719
HphI GGTGA 5 cut(s) 1136, 1796, 2099, 2990, 3163
Hpy166II GTNNAC 6 cut(s) 260, 1115, 1838, 2228, 2389, 3280
Hpy8I GTNNAC 6 cut(s) 260, 1115, 1838, 2228, 2389, 3280
Hpy99I CGWCG 2 cut(s) 35, 189
HpyAV CCTTC 7 cut(s) 520, 953, 1100, 1753, 1867, 1893, 3244
HpyCH4III ACNGT 9 cut(s) 1401, 1512, 1547, 1568, 2111, 2232, 2263, 2446, 2744
HpyCH4IV ACGT 4 cut(s) 516, 997, 2691, 3246
HpyF10VI GCNNNNNNNGC 8 cut(s) 24, 33, 586, 699, 786, 1014, 1473, 2203
HpySE526I ACGT 4 cut(s) 516, 997, 2691, 3246
HspAI GCGC 5 cut(s) 25, 418, 466, 610, 1369
KflI GGGWCCC 1 cut(s) 2039
KpnI GGTACC 1 cut(s) 718
Ksp22I TGATCA 2 cut(s) 2347, 2890
LguI GCTCTTC 1 cut(s) 1896
LweI GCATC 5 cut(s) 375, 1428, 1454, 2113, 2662
MaeI CTAG 6 cut(s) 255, 351, 399, 543, 819, 920
MaeII ACGT 4 cut(s) 516, 997, 2691, 3246
MaeIII GTNAC 3 cut(s) 764, 1952, 2095
MbiI CCGCTC 1 cut(s) 562
MfeI CAATTG 1 cut(s) 1012
MflI RGATCY 1 cut(s) 2627
MhlI GDGCHC 5 cut(s) 284, 516, 914, 1168, 3219
MlsI TGGCCA 1 cut(s) 1347
MluNI TGGCCA 1 cut(s) 1347
MlyI GAGTC 4 cut(s) 938, 2134, 2708, 3275
MmeI TCCRAC 2 cut(s) 2245, 2540
Mox20I TGGCCA 1 cut(s) 1347
MroXI GAANNNNTTC 3 cut(s) 1608, 3084, 3102
MscI TGGCCA 1 cut(s) 1347
MseI TTAA 6 cut(s) 1403, 1737, 2507, 2807, 2850, 2964
MslI CAYNNNNRTG 3 cut(s) 1845, 2568, 2612
Msp20I TGGCCA 1 cut(s) 1347
MspA1I CMGCKG 5 cut(s) 18, 159, 640, 850, 3185
MspI CCGG 3 cut(s) 11, 533, 719
MspR9I CCNGG 9 cut(s) 12, 495, 591, 719, 720, 1119, 1349, 2664, 2792
MunI CAATTG 1 cut(s) 1012
Mva1269I GAATGC 2 cut(s) 1311, 1579
MvaI CCWGG 6 cut(s) 495, 591, 1119, 1349, 2664, 2792
MvnI CGCG 3 cut(s) 420, 468, 612
MwoI GCNNNNNNNGC 8 cut(s) 24, 33, 586, 699, 786, 1014, 1473, 2203
NciI CCSGG 3 cut(s) 12, 719, 720
NmuCI GTSAC 3 cut(s) 764, 1952, 2095
NspI RCATGY 3 cut(s) 1220, 2471, 2597
PagI TCATGA 2 cut(s) 2680, 2887
PciI ACATGT 1 cut(s) 1216
PciSI GCTCTTC 1 cut(s) 1896
PctI GAATGC 2 cut(s) 1311, 1579
PdmI GAANNNNTTC 3 cut(s) 1608, 3084, 3102
PfeI GAWTC 9 cut(s) 181, 1297, 1487, 2018, 2064, 2090, 2423, 2723, 2867
PflMI CCANNNNNTGG 1 cut(s) 2305
PfoI TCCNGGA 1 cut(s) 2662
PleI GAGTC 4 cut(s) 937, 2134, 2707, 3275
PmaCI CACGTG 2 cut(s) 517, 3247
PmlI CACGTG 2 cut(s) 517, 3247
PpsI GAGTC 4 cut(s) 937, 2134, 2707, 3275
Ppu21I YACGTR 2 cut(s) 517, 3247
PpuMI RGGWCCY 1 cut(s) 2039
PscI ACATGT 1 cut(s) 1216
PshBI ATTAAT 1 cut(s) 2850
Psp124BI GAGCTC 3 cut(s) 284, 914, 1168
Psp5II RGGWCCY 1 cut(s) 2039
Psp6I CCWGG 6 cut(s) 493, 589, 1117, 1347, 2662, 2790
PspCI CACGTG 2 cut(s) 517, 3247
PspGI CCWGG 6 cut(s) 493, 589, 1117, 1347, 2662, 2790
PspPI GGNCC 5 cut(s) 13, 1228, 1722, 1762, 2039
PspPPI RGGWCCY 1 cut(s) 2039
PstI CTGCAG 2 cut(s) 294, 342
PstNI CAGNNNCTG 1 cut(s) 1047
PsuI RGATCY 1 cut(s) 2627
PvuII CAGCTG 1 cut(s) 3185
RsaI GTAC 6 cut(s) 259, 679, 716, 860, 2234, 2320
RsaNI GTAC 6 cut(s) 258, 678, 715, 859, 2233, 2319
RseI CAYNNNNRTG 3 cut(s) 1845, 2568, 2612
SacI GAGCTC 3 cut(s) 284, 914, 1168
SapI GCTCTTC 1 cut(s) 1896
SaqAI TTAA 6 cut(s) 1403, 1737, 2507, 2807, 2850, 2964
Sau96I GGNCC 5 cut(s) 13, 1228, 1722, 1762, 2039
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 4 cut(s) 938, 2134, 2708, 3275
ScrFI CCNGG 9 cut(s) 12, 495, 591, 719, 720, 1119, 1349, 2664, 2792
SduI GDGCHC 5 cut(s) 284, 516, 914, 1168, 3219
SfaNI GCATC 5 cut(s) 375, 1428, 1454, 2113, 2662
SfcI CTRYAG 4 cut(s) 290, 338, 1155, 2862
SinI GGWCC 1 cut(s) 2039
SmaI CCCGGG 1 cut(s) 720
SmiMI CAYNNNNRTG 3 cut(s) 1845, 2568, 2612
SmlI CTYRAG 3 cut(s) 854, 1458, 3218
SmoI CTYRAG 3 cut(s) 854, 1458, 3218
SspI AATATT 1 cut(s) 1801
SspMI CTAG 6 cut(s) 255, 351, 399, 543, 819, 920
SstI GAGCTC 3 cut(s) 284, 914, 1168
StyD4I CCNGG 9 cut(s) 10, 493, 589, 717, 718, 1117, 1347, 2662, 2790
StyI CCWWGG 7 cut(s) 1491, 1913, 2168, 2758, 2954, 2974, 3132
TaaI ACNGT 9 cut(s) 1401, 1512, 1547, 1568, 2111, 2232, 2263, 2446, 2744
TaiI ACGT 4 cut(s) 519, 1000, 2694, 3249
TaqI TCGA 5 cut(s) 187, 236, 284, 380, 3088
TatI WGTACW 5 cut(s) 257, 677, 858, 2232, 2318
TauI GCSGC 5 cut(s) 18, 65, 853, 1532, 2790
TfiI GAWTC 9 cut(s) 181, 1297, 1487, 2018, 2064, 2090, 2423, 2723, 2867
Tru1I TTAA 6 cut(s) 1403, 1737, 2507, 2807, 2850, 2964
Tru9I TTAA 6 cut(s) 1403, 1737, 2507, 2807, 2850, 2964
TscAI CASTG 9 cut(s) 638, 841, 1552, 1625, 1930, 1957, 2100, 2114, 2877
TseFI GTSAC 3 cut(s) 764, 1952, 2095
Tsp45I GTSAC 3 cut(s) 764, 1952, 2095
TspGWI ACGGA 1 cut(s) 2624
TspMI CCCGGG 1 cut(s) 718
TspRI CASTG 9 cut(s) 638, 841, 1552, 1625, 1930, 1957, 2100, 2114, 2877
Van91I CCANNNNNTGG 1 cut(s) 2305
VpaK11BI GGWCC 1 cut(s) 2039
VspI ATTAAT 1 cut(s) 2850
XagI CCTNNNNNAGG 1 cut(s) 2978
XapI RAATTY 4 cut(s) 598, 1928, 2837, 2939
XceI RCATGY 3 cut(s) 1220, 2471, 2597
XmaI CCCGGG 1 cut(s) 718
XmiI GTMKAC 1 cut(s) 1837
XmnI GAANNNNTTC 3 cut(s) 1608, 3084, 3102
XspI CTAG 6 cut(s) 255, 351, 399, 543, 819, 920
ZrmI AGTACT 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.