Rroxscaffold_4G00296770
ERF Family

A domain family that is part of the cupin metalloenzyme superfamily.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002674
Physical Location & Seq
Forward (+)
16814016 .. 16822923
8908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296770.1

Sequence Viewer

Length: 3273 bp
ATGGAATCATCGCCGCCGCACGACCGCCGGTGCAAGCGCGTCGCCGGCGCCGGCCCGAAGTCGCGGCCCGGCGAGCTCAAGAGAAACCGGGATAAGAAGATCAAGAGAGACAGTGAAGTGGTGGAGGAGGAGGATCGCCGCTGCAATCGCCGCGCCGGAGCGTGGCGGTGCGGCGAGCCGGCGGCGGTTGGGAAGCCGATGTGTCGACATCACTTGGCGCAGAGCAAGAGTTACCAGGAGAGGAAGAAAAAGAGAGAGGGAAGAGGAGACAGTGATTCCGGCGAAGAAGACGGACGTGACCGGAAAATCAACGCCGGCGGATCGGAGAGTAAGAAGAAGAAGAAGAGGAGGAGGAGGACCGCGAGTGAGTCTGAGTCGGAGTCAGAACACAGGTCGAGCAATCGGACTGTGAAAGCGAAGGTGAATGGTAAGTCTGCAGATTCCGGTAGTGTTATGAAGAAGAGTAAACTAAAAGAGGAAAAGCCAATGGAAAAGGATAAGTCGAACAAAATCAAGGGGAGCTTGATGTGTCACCAATGTCAGCGGAACGATAAGAGTGGTGTTGTACACTGCTCTCTGTGTAAATCAAAGCGGTTTTGCTATGAGTGCATTGAAAGATGGTACCCTGGGAAATCAAGAGAGGAATTTGAGGATGCTTGCCCATTTTGCCGTGGCAACTGCAACTGCAAAGCGTGCCTGCGCGAGTTTTTGGTTAAACCTTGCCGAGAAGTTGAGCCCAGTGTTAAGTTACAGCGACTGCGTTACCTGTTATATAAGGCTTTGCCTGTGCTGAGGCATATATATAGTGAGCAGAGCTCTGAACTGGAGATAGAAGCCAAAATCAGAGGTGTACACTTGACTGAAATGGACATAAAGAGAACTAAAGTACACCAAAATGAGCGAATGTACTGTGACAATTGTTCTACATCTATTGTGGATTTCCACAGAAGCTGTCCAAACCCCAATTGTTCCTATGACCTGTGTCTTACTTGTTGCAAAGAGCTAAGGGACGGTCGGCAACCTGGAGGTAATGAAGGAGAAACATCTCACCAGCAAGCTCTAGAAAGAGCTCATAAAGAAGTGAAAGGACATTGCTGGGAGAGTAAAGTTGCCTCTACTTCTGATGATTCTAAAGTGGACCCATCCATTTCTTTTCCTAATTGGAGAGCTGATTCTGATGGCAGTATTCCATGCCCTCCAAAAGAACGTGGAGGTTGTGGCAATGCGAAGCTGGAATTGAGGCGTAAATTCAAAGCTAATTGGGTAATGAAGCTGCTAAAAAATGCAGAAGATGTTACTACTGACTTCAAGTGGCAAGAAGTTGACATTTCTCAAGGATGTTCCTGGTGCCAGCCAAATGATTCTGAGGAAACCAATGATAGGAAGCCTGAAAGGAGGCAAGCAGCTTTCAGGAAAAACAGTCATGATAACTTCTTATACTGTCCAGATGCTATTGACATTTCAGACGATGAGATTGAGCATTTTCAGAGGCACTGGATGAAAGGGGAACCGGTGATTGTCAGAAATGTTCTTGACAAGACATCTGGTCTTAGTTGGGAACCTATGGTTATGTGGAGGGCTTTTAGAGAAACAGGTGCTAAAGTAAAGTTCAAAGAGGAGACTAAAAGTGTAAAAGCCATTGATTGCTTGGATTGGAATGAGGTTGAGATTAATATTCACCAATTTTTCACGGGCTACTTAGCGGGTCGCATGCATAAAGATAAGTGGCCAGAGATGTTAAAATTAAAGGATTGGCCGTCATCAACTTTGTTTGAGGAACGCTTGCCAAGACACTGTGCGGAATTCATTGCAGCGCTCCCCTACAGTGACTATACTGATCCCAAAGATTCAAATGCTGGTATTCTCAATCTTGCTACAAGACTTCCTGAAGGGTCGCTGAAACCTGATATGGGACCAAAAACATACATTGCTTATGGATTCTCCGAAGAGCTTGGTAGAGGTGATTCAGTGACTAAGCTGCACTGCGATATGTCTGATGCGGTAAATGTTCTGACACACACAGCAACAGTGAAGATTGAGCCGTGGCAGCACAATGCCATAAAGAATTTGAAGAGGAAACATGAAGCTCAAGACCTTTGTGAACTCTACAAAGAAAAAAATCACGAAAGGGGCAGGGTTAGAGGAACATTGTTGAAGAAAACTCGCAACGTCCTGGTTAAGGGTGCTGATTCTTCTGAATATACTGAAAGTGGTAAAGTTGTGGAGAATGACCATCTAGTGTCAACTCTGCAGGATGGGAAGGGAAACAAACTGGACAGAGCACAATCATGTAGAACTTCACTTTTGTCAGATTCTGTCGATCCAGGCATCCTTAGATCTGATGAAACAGAATATGTTCCGGAACCTCTTGCTACTACTGAACTAAATGAAGTAAAGGTTGCGAGTGTTGATCATCAGAGGAATGATGTGCTAAAATCCACCTTGCCTCGTGCAAATGGAGTTGAAAAAGAGCAAGAGTGTGTTCAATGTTCCAGTGACATGATCAGTGGAAGGCTTGAAGGGAAAGATGCTTCAAGGGATGCAAGTGTAAACACGCATGCAACAAAGGATTTCAAAGGTAGTGACAAGTTGGACATTGTCCATGCTGGTGCTGTTTGGGACATCTTTCGTATCCAGGACACTCCCAAGTTAATAGAGTACCTGGAGAAACACAAAAAAGAATTTCGCCACCTTAATAATCATCCTGTAGATTCTGTTGTTCATCCTATTCATGATCAGACTCTGTACTTGAATGAGAGACATAAGAAACAACTAAAGGAGGAGTTCGATGTGGAGCCTTGGACATTTGAGCAACATCTTGGTGAGGCAGTTTTTATTCCAGCGGGATGTCCTCATCAAGTGAGAAATAGACAATCTTGTATAAAGGTAGCACTTGATTTTGTATCTCCTGAGAATCTTGAAGTATGTCTGCGATTAACTGAAGAGTTCCGTTTACTCCCAAAAACCCACAGAGCCAAGGAAGATAAATTGGAGGTGAAGAAGATGACACTCTATGCTGTAAGTTCAGCACTGCGAGAAGCCAAAAATTTGATGCTGGGGCTTGGGCTCCTTGCAAAGTGCCATATAAGTAGTTTATTGTTGGTGATAATTAAAAGTTGGAATGCAAAACAATTCTGTGGTATTATCCCATCTGTAAGTATGAGGAAGTTGGAGATTCCAAGGTCATGTCCGGGCAATAGTAGGGAGATGAAACATCAGATGGGGAACGTTGGAGAATGGAGTCCCCAGTCTGCCAATACAGGCCTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048522 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC WRC zf-4CXXC_R1

Protein Analysis

1090

Amino Acids

124.35

Weight (kDa)

8.78

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 871 - 967 2.1e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 621
AccB1I GGYRCC 3 cut(s) 47, 621, 1347
AccI GTMKAC 1 cut(s) 205
AccII CGCG 5 cut(s) 39, 64, 153, 362, 702
AccIII TCCGGA 1 cut(s) 2359
AclI AACGTT 1 cut(s) 3228
AclWI GGATC 4 cut(s) 141, 328, 1832, 2315
AcoI YGGCCR 2 cut(s) 1727, 1754
AcsI RAATTY 6 cut(s) 644, 1247, 1802, 2065, 2681, 3046
AcuI CTGAAG 2 cut(s) 1908, 2961
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 7 cut(s) 567, 623, 852, 888, 908, 2660, 2747
AfeI AGCGCT 1 cut(s) 1815
AfiI CCNNNNNNNGG 4 cut(s) 162, 1380, 1910, 2179
AgeI ACCGGT 1 cut(s) 1510
AhdI GACNNNNNGTC 1 cut(s) 1545
AjiI CACGTC 1 cut(s) 296
AjnI CCWGG 8 cut(s) 234, 625, 1021, 1343, 2172, 2323, 2634, 2661
Alw21I GWGCWC 4 cut(s) 78, 818, 1072, 2284
Alw26I GTCTC 4 cut(s) 102, 261, 1613, 2752
AlwI GGATC 4 cut(s) 141, 328, 1832, 2315
AlwNI CAGNNNCTG 4 cut(s) 757, 951, 2315, 2743
Aor13HI TCCGGA 1 cut(s) 2359
Aor51HI AGCGCT 1 cut(s) 1815
AoxI GGCC 5 cut(s) 52, 65, 1727, 1754, 3262
ApeKI GCWGC 6 cut(s) 141, 1273, 1403, 1811, 1978, 2047
ApoI RAATTY 6 cut(s) 644, 1247, 1802, 2065, 2681, 3046
AseI ATTAAT 1 cut(s) 1671
AsiGI ACCGGT 1 cut(s) 1510
Asp700I GAANNNNTTC 1 cut(s) 2355
Asp718I GGTACC 1 cut(s) 621
AspLEI GCGC 6 cut(s) 39, 50, 155, 220, 702, 1816
AspS9I GGNCC 5 cut(s) 53, 66, 357, 1138, 1913
AsuC2I CCSGG 3 cut(s) 69, 89, 3192
AsuHPI GGTGA 9 cut(s) 433, 524, 1040, 1525, 1670, 1973, 2834, 3007, 3115
AvaII GGWCC 3 cut(s) 357, 1138, 1913
BalI TGGCCA 1 cut(s) 1729
BanI GGYRCC 3 cut(s) 47, 621, 1347
BanII GRGCYC 5 cut(s) 78, 738, 818, 1072, 3069
BauI CACGAG 1 cut(s) 2448
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 4 cut(s) 78, 818, 1072, 2284
BbvCI CCTCAGC 1 cut(s) 791
BbvI GCAGC 6 cut(s) 128, 1260, 1415, 1823, 1965, 2059
BccI CCATC 7 cut(s) 612, 1150, 1172, 2241, 2249, 3157, 3214
BceAI ACGGC 3 cut(s) 654, 1741, 2026
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 8 cut(s) 236, 627, 1023, 1345, 2174, 2325, 2636, 2663
BciVI GTATCC 1 cut(s) 2642
BclI TGATCA 3 cut(s) 2410, 2502, 2734
BcnI CCSGG 3 cut(s) 69, 89, 3192
BcoDI GTCTC 4 cut(s) 102, 261, 1613, 2752
BfaI CTAG 2 cut(s) 1061, 2237
BfmI CTRYAG 4 cut(s) 435, 1822, 2249, 2706
BfoI RGCGCY 2 cut(s) 51, 1817
BfuI GTATCC 1 cut(s) 2642
BglII AGATCT 1 cut(s) 2336
Bme18I GGWCC 3 cut(s) 357, 1138, 1913
BmeRI GACNNNNNGTC 1 cut(s) 1545
BmgBI CACGTC 1 cut(s) 296
BmgT120I GGNCC 5 cut(s) 53, 66, 357, 1138, 1913
BmrI ACTGGG 2 cut(s) 732, 3241
BmsI GCATC 7 cut(s) 643, 1438, 1987, 2337, 2518, 2530, 3042
BmuI ACTGGG 2 cut(s) 732, 3241
BoxI GACNNNNGTC 1 cut(s) 981
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 800, 832
BpmI CTGGAG 3 cut(s) 845, 1044, 2684
Bpu10I CCTNAGC 2 cut(s) 791, 1004
BpuEI CTTGAG 3 cut(s) 62, 1317, 2073
BpuMI CCSGG 3 cut(s) 69, 89, 3192
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 7 cut(s) 625, 626, 670, 2042, 2798, 2976, 3179
BsaWI WCCGGW 4 cut(s) 300, 443, 1510, 2359
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 1380, 1910, 2179
Bse118I RCCGGY 6 cut(s) 27, 44, 50, 178, 314, 1510
Bse1I ACTGG 6 cut(s) 738, 828, 1499, 2277, 2493, 3247
Bse3DI GCAATG 4 cut(s) 1090, 1228, 1806, 1926
BseAI TCCGGA 1 cut(s) 2359
BseBI CCWGG 8 cut(s) 236, 627, 1023, 1345, 2174, 2325, 2636, 2663
BseDI CCNNGG 7 cut(s) 625, 626, 670, 2042, 2798, 2976, 3179
BseLI CCNNNNNNNGG 4 cut(s) 162, 1380, 1910, 2179
BseMI GCAATG 4 cut(s) 1090, 1228, 1806, 1926
BseMII CTCAG 4 cut(s) 363, 782, 1356, 2901
BseNI ACTGG 6 cut(s) 738, 828, 1499, 2277, 2493, 3247
BseRI GAGGAG 8 cut(s) 140, 143, 279, 361, 364, 367, 1631, 2795
BseXI GCAGC 6 cut(s) 128, 1260, 1415, 1823, 1965, 2059
BseYI CCCAGC 2 cut(s) 1095, 3055
BsgI GTGCAG 1 cut(s) 1964
Bsh1236I CGCG 5 cut(s) 39, 64, 153, 362, 702
Bsh1285I CGRYCG 2 cut(s) 25, 1015
BshFI GGCC 5 cut(s) 54, 67, 1729, 1756, 3264
BshNI GGYRCC 3 cut(s) 47, 621, 1347
BshTI ACCGGT 1 cut(s) 1510
BsiEI CGRYCG 2 cut(s) 25, 1015
BsiHKAI GWGCWC 4 cut(s) 78, 818, 1072, 2284
BslFI GGGAC 4 cut(s) 1022, 1926, 2633, 3228
BslI CCNNNNNNNGG 4 cut(s) 162, 1380, 1910, 2179
BsmAI GTCTC 4 cut(s) 102, 261, 1613, 2752
BsmFI GGGAC 4 cut(s) 1022, 1926, 2633, 3228
BsmI GAATGC 1 cut(s) 3127
BsnI GGCC 5 cut(s) 54, 67, 1729, 1756, 3264
Bsp1286I GDGCHC 6 cut(s) 78, 738, 818, 1072, 2284, 3069
Bsp13I TCCGGA 1 cut(s) 2359
Bsp1407I TGTACA 2 cut(s) 565, 850
Bsp143I GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
BspANI GGCC 5 cut(s) 54, 67, 1729, 1756, 3264
BspCNI CTCAG 4 cut(s) 364, 783, 1357, 2902
BspEI TCCGGA 1 cut(s) 2359
BspFNI CGCG 5 cut(s) 39, 64, 153, 362, 702
BspHI TCATGA 2 cut(s) 1423, 2731
BspMAI CTGCAG 2 cut(s) 439, 2253
BspPI GGATC 4 cut(s) 141, 328, 1832, 2315
BspQI GCTCTTC 1 cut(s) 1941
BspT107I GGYRCC 3 cut(s) 47, 621, 1347
BsrDI GCAATG 4 cut(s) 1090, 1228, 1806, 1926
BsrFI RCCGGY 6 cut(s) 27, 44, 50, 178, 314, 1510
BsrGI TGTACA 2 cut(s) 565, 850
BsrI ACTGG 6 cut(s) 738, 828, 1499, 2277, 2493, 3247
BssAI RCCGGY 6 cut(s) 27, 44, 50, 178, 314, 1510
BssECI CCNNGG 7 cut(s) 625, 626, 670, 2042, 2798, 2976, 3179
BssMI GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 2448
BssT1I CCWWGG 3 cut(s) 2798, 2976, 3179
Bst2BI CACGAG 1 cut(s) 2448
Bst2UI CCWGG 8 cut(s) 236, 627, 1023, 1345, 2174, 2325, 2636, 2663
Bst6I CTCTTC 6 cut(s) 256, 338, 455, 1941, 2066, 2937
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 693
BstAUI TGTACA 2 cut(s) 565, 850
BstDEI CTNAG 9 cut(s) 372, 791, 1004, 1365, 1550, 1699, 1974, 2333, 2910
BstDSI CCRYGG 2 cut(s) 670, 2042
BstENI CCTNNNNNAGG 1 cut(s) 2177
BstFNI CGCG 5 cut(s) 39, 64, 153, 362, 702
BstH2I RGCGCY 2 cut(s) 51, 1817
BstHHI GCGC 6 cut(s) 39, 50, 155, 220, 702, 1816
BstKTI GATC 9 cut(s) 102, 136, 323, 1840, 2323, 2339, 2413, 2505, 2737
BstMAI GTCTC 4 cut(s) 102, 261, 1613, 2752
BstMBI GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
BstMCI CGRYCG 2 cut(s) 25, 1015
BstMWI GCNNNNNNNGC 8 cut(s) 45, 73, 147, 150, 606, 666, 693, 2047
BstNI CCWGG 8 cut(s) 236, 627, 1023, 1345, 2174, 2325, 2636, 2663
BstNSI RCATGY 2 cut(s) 1714, 2561
BstPAI GACNNNNGTC 1 cut(s) 981
BstSFI CTRYAG 4 cut(s) 435, 1822, 2249, 2706
BstUI CGCG 5 cut(s) 39, 64, 153, 362, 702
BstV1I GCAGC 6 cut(s) 128, 1260, 1415, 1823, 1965, 2059
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 2336
BstYI RGATCY 1 cut(s) 2336
BsuI GTATCC 1 cut(s) 2642
BsuRI GGCC 5 cut(s) 54, 67, 1729, 1756, 3264
BtgI CCRYGG 2 cut(s) 670, 2042
BtrI CACGTC 1 cut(s) 296
BtsI GCAGTG 3 cut(s) 568, 1981, 3029
CaiI CAGNNNCTG 4 cut(s) 757, 951, 2315, 2743
CciI TCATGA 2 cut(s) 1423, 2731
CfoI GCGC 6 cut(s) 39, 50, 155, 220, 702, 1816
Cfr10I RCCGGY 6 cut(s) 27, 44, 50, 178, 314, 1510
Cfr13I GGNCC 5 cut(s) 53, 66, 357, 1138, 1913
CseI GACGC 1 cut(s) 28
Csp6I GTAC 7 cut(s) 566, 622, 851, 887, 907, 2659, 2746
CspAI ACCGGT 1 cut(s) 1510
CviQI GTAC 7 cut(s) 566, 622, 851, 887, 907, 2659, 2746
DdeI CTNAG 9 cut(s) 372, 791, 1004, 1365, 1550, 1699, 1974, 2333, 2910
DinI GGCGCC 1 cut(s) 49
DpnI GATC 9 cut(s) 101, 135, 322, 1839, 2322, 2338, 2412, 2504, 2736
DpnII GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
DriI GACNNNNNGTC 1 cut(s) 1545
EaeI YGGCCR 2 cut(s) 1727, 1754
Eam1104I CTCTTC 6 cut(s) 256, 338, 455, 1941, 2066, 2937
Eam1105I GACNNNNNGTC 1 cut(s) 1545
EarI CTCTTC 6 cut(s) 256, 338, 455, 1941, 2066, 2937
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 3 cut(s) 76, 816, 1070
Eco130I CCWWGG 3 cut(s) 2798, 2976, 3179
Eco147I AGGCCT 1 cut(s) 3264
Eco24I GRGCYC 5 cut(s) 78, 738, 818, 1072, 3069
Eco47I GGWCC 3 cut(s) 357, 1138, 1913
Eco47III AGCGCT 1 cut(s) 1815
Eco53kI GAGCTC 3 cut(s) 76, 816, 1070
Eco57I CTGAAG 2 cut(s) 1908, 2961
EcoICRI GAGCTC 3 cut(s) 76, 816, 1070
EcoNI CCTNNNNNAGG 1 cut(s) 2177
EcoRI GAATTC 1 cut(s) 1802
EcoRII CCWGG 8 cut(s) 234, 625, 1021, 1343, 2172, 2323, 2634, 2661
EcoT14I CCWWGG 3 cut(s) 2798, 2976, 3179
EcoT22I ATGCAT 1 cut(s) 1716
EcoT38I GRGCYC 5 cut(s) 78, 738, 818, 1072, 3069
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
ErhI CCWWGG 3 cut(s) 2798, 2976, 3179
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FaqI GGGAC 4 cut(s) 1022, 1926, 2633, 3228
FauI CCCGC 2 cut(s) 1696, 2836
FbaI TGATCA 3 cut(s) 2410, 2502, 2734
FblI GTMKAC 1 cut(s) 205
FriOI GRGCYC 5 cut(s) 78, 738, 818, 1072, 3069
FspBI CTAG 2 cut(s) 1061, 2237
GlaI GCGC 6 cut(s) 38, 49, 154, 219, 701, 1815
GsaI CCCAGC 2 cut(s) 1099, 3059
GsuI CTGGAG 3 cut(s) 845, 1044, 2684
HaeII RGCGCY 2 cut(s) 51, 1817
HaeIII GGCC 5 cut(s) 54, 67, 1729, 1756, 3264
HgaI GACGC 1 cut(s) 28
HhaI GCGC 6 cut(s) 39, 50, 155, 220, 702, 1816
Hin1I GRCGYC 1 cut(s) 48
Hin6I GCGC 6 cut(s) 37, 48, 153, 218, 700, 1814
HinP1I GCGC 6 cut(s) 37, 48, 153, 218, 700, 1814
HincII GTYRAC 3 cut(s) 206, 1324, 2244
HindII GTYRAC 3 cut(s) 206, 1324, 2244
HphI GGTGA 9 cut(s) 433, 524, 1040, 1525, 1670, 1973, 2834, 3007, 3115
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 6 cut(s) 412, 1028, 1883, 2254, 2505, 2513
HpyCH4IV ACGT 4 cut(s) 295, 1207, 2169, 3228
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 73, 147, 150, 606, 666, 693, 2047
HpyF3I CTNAG 9 cut(s) 372, 791, 1004, 1365, 1550, 1699, 1974, 2333, 2910
HpySE526I ACGT 4 cut(s) 295, 1207, 2169, 3228
Hsp92I GRCGYC 1 cut(s) 48
HspAI GCGC 6 cut(s) 37, 48, 153, 218, 700, 1814
KasI GGCGCC 1 cut(s) 47
Kpn2I TCCGGA 1 cut(s) 2359
KpnI GGTACC 1 cut(s) 625
KroI GCCGGC 4 cut(s) 44, 50, 178, 314
KroNI GCCGGC 4 cut(s) 46, 52, 180, 316
Ksp22I TGATCA 3 cut(s) 2410, 2502, 2734
Kzo9I GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
LguI GCTCTTC 1 cut(s) 1941
LmnI GCTCC 5 cut(s) 158, 519, 1821, 2794, 3072
Lsp1109I GCAGC 6 cut(s) 128, 1260, 1415, 1823, 1965, 2059
LweI GCATC 7 cut(s) 643, 1438, 1987, 2337, 2518, 2530, 3042
MaeI CTAG 2 cut(s) 1061, 2237
MaeII ACGT 4 cut(s) 295, 1207, 2169, 3228
MalI GATC 9 cut(s) 101, 135, 322, 1839, 2322, 2338, 2412, 2504, 2736
MboI GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
MfeI CAATTG 2 cut(s) 916, 964
MflI RGATCY 1 cut(s) 2336
MhlI GDGCHC 6 cut(s) 78, 738, 818, 1072, 2284, 3069
MlsI TGGCCA 1 cut(s) 1729
MluNI TGGCCA 1 cut(s) 1729
Mly113I GGCGCC 1 cut(s) 48
MlyI GAGTC 5 cut(s) 377, 383, 389, 2734, 3250
MmeI TCCRAC 5 cut(s) 357, 2571, 3098, 3150, 3211
Mox20I TGGCCA 1 cut(s) 1729
Mph1103I ATGCAT 1 cut(s) 1716
MreI CGCCGGCG 2 cut(s) 44, 314
MroI TCCGGA 1 cut(s) 2359
MroNI GCCGGC 4 cut(s) 44, 50, 178, 314
MroXI GAANNNNTTC 1 cut(s) 2355
MscI TGGCCA 1 cut(s) 1729
MslI CAYNNNNRTG 4 cut(s) 894, 2287, 2607, 2820
Msp20I TGGCCA 1 cut(s) 1729
MspA1I CMGCKG 3 cut(s) 141, 544, 2843
MunI CAATTG 2 cut(s) 916, 964
Mva1269I GAATGC 1 cut(s) 3127
MvaI CCWGG 8 cut(s) 236, 627, 1023, 1345, 2174, 2325, 2636, 2663
MvnI CGCG 5 cut(s) 39, 64, 153, 362, 702
MwoI GCNNNNNNNGC 8 cut(s) 45, 73, 147, 150, 606, 666, 693, 2047
NaeI GCCGGC 4 cut(s) 46, 52, 180, 316
NarI GGCGCC 1 cut(s) 48
NciI CCSGG 3 cut(s) 69, 89, 3192
NdeII GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
NgoMIV GCCGGC 4 cut(s) 44, 50, 178, 314
NmeAIII GCCGAG 1 cut(s) 749
NmuCI GTSAC 7 cut(s) 296, 530, 911, 1826, 1969, 2495, 2582
NsiI ATGCAT 1 cut(s) 1716
NspI RCATGY 2 cut(s) 1714, 2561
PaeI GCATGC 2 cut(s) 1714, 2561
PagI TCATGA 2 cut(s) 1423, 2731
PasI CCCWGGG 1 cut(s) 626
PceI AGGCCT 1 cut(s) 3264
PciSI GCTCTTC 1 cut(s) 1941
PctI GAATGC 1 cut(s) 3127
PdiI GCCGGC 4 cut(s) 46, 52, 180, 316
PdmI GAANNNNTTC 1 cut(s) 2355
PflFI GACNNNGTC 1 cut(s) 2597
PfoI TCCNGGA 1 cut(s) 2634
PinAI ACCGGT 1 cut(s) 1510
PleI GAGTC 5 cut(s) 376, 382, 388, 2734, 3249
PluTI GGCGCC 1 cut(s) 51
PpsI GAGTC 5 cut(s) 376, 382, 388, 2734, 3249
PshAI GACNNNNGTC 1 cut(s) 981
PshBI ATTAAT 1 cut(s) 1671
Psp124BI GAGCTC 3 cut(s) 78, 818, 1072
Psp1406I AACGTT 1 cut(s) 3228
Psp6I CCWGG 8 cut(s) 234, 625, 1021, 1343, 2172, 2323, 2634, 2661
PspFI CCCAGC 2 cut(s) 1095, 3055
PspGI CCWGG 8 cut(s) 234, 625, 1021, 1343, 2172, 2323, 2634, 2661
PspPI GGNCC 5 cut(s) 53, 66, 357, 1138, 1913
PstI CTGCAG 2 cut(s) 439, 2253
PstNI CAGNNNCTG 4 cut(s) 757, 951, 2315, 2743
PsuI RGATCY 1 cut(s) 2336
PsyI GACNNNGTC 1 cut(s) 2597
RsaI GTAC 7 cut(s) 567, 623, 852, 888, 908, 2660, 2747
RsaNI GTAC 7 cut(s) 566, 622, 851, 887, 907, 2659, 2746
RseI CAYNNNNRTG 4 cut(s) 894, 2287, 2607, 2820
SacI GAGCTC 3 cut(s) 78, 818, 1072
SalI GTCGAC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 1941
Sau3AI GATC 9 cut(s) 99, 133, 320, 1837, 2320, 2336, 2410, 2502, 2734
Sau96I GGNCC 5 cut(s) 53, 66, 357, 1138, 1913
SchI GAGTC 5 cut(s) 377, 383, 389, 2734, 3250
SduI GDGCHC 6 cut(s) 78, 738, 818, 1072, 2284, 3069
SfaNI GCATC 7 cut(s) 643, 1438, 1987, 2337, 2518, 2530, 3042
SfcI CTRYAG 4 cut(s) 435, 1822, 2249, 2706
SfoI GGCGCC 1 cut(s) 49
SgrAI CRCCGGYG 3 cut(s) 27, 44, 314
SinI GGWCC 3 cut(s) 357, 1138, 1913
SmiMI CAYNNNNRTG 4 cut(s) 894, 2287, 2607, 2820
SmlI CTYRAG 3 cut(s) 77, 1332, 2088
SmoI CTYRAG 3 cut(s) 77, 1332, 2088
SphI GCATGC 2 cut(s) 1714, 2561
SseBI AGGCCT 1 cut(s) 3264
SspDI GGCGCC 1 cut(s) 47
SspI AATATT 1 cut(s) 1675
SspMI CTAG 2 cut(s) 1061, 2237
SstI GAGCTC 3 cut(s) 78, 818, 1072
StuI AGGCCT 1 cut(s) 3264
StyI CCWWGG 3 cut(s) 2798, 2976, 3179
TaiI ACGT 4 cut(s) 298, 1210, 2172, 3231
TaqI TCGA 5 cut(s) 205, 395, 503, 2319, 2787
TatI WGTACW 5 cut(s) 565, 850, 886, 906, 2745
TauI GCSGC 7 cut(s) 16, 19, 67, 141, 153, 174, 185
TseFI GTSAC 7 cut(s) 296, 530, 911, 1826, 1969, 2495, 2582
TseI GCWGC 6 cut(s) 141, 1273, 1403, 1811, 1978, 2047
Tsp45I GTSAC 7 cut(s) 296, 530, 911, 1826, 1969, 2495, 2582
TspGWI ACGGA 2 cut(s) 306, 2939
Tth111I GACNNNGTC 1 cut(s) 2597
VpaK11BI GGWCC 3 cut(s) 357, 1138, 1913
VspI ATTAAT 1 cut(s) 1671
XagI CCTNNNNNAGG 1 cut(s) 2177
XapI RAATTY 6 cut(s) 644, 1247, 1802, 2065, 2681, 3046
XbaI TCTAGA 1 cut(s) 1060
XceI RCATGY 2 cut(s) 1714, 2561
XcmI CCANNNNNNNNNTGG 1 cut(s) 1645
XmiI GTMKAC 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 2355
XspI CTAG 2 cut(s) 1061, 2237
Zsp2I ATGCAT 1 cut(s) 1716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.