Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009625

Physical Location & Seq

Genomic Coordinates Reverse (-)

21283764 .. 21297311

Gene Structure 13548 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1099700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGCGCTCTCGCGGCTTCGACACCCAGTGATCTCCCGCGCGCCATCTCTCCTGAGAGCTCGCCTTCTCTCCTCGTCCACCAGATCACTCACTCGAACTTTCAGTTTGCAAAATTCTACTGCGCTTGGTGATAATACGCTTTTAAGGCCAGCATCTTTGTCCATGCTCACTGGAGTTCATGATATACCATCAAAGCTGAAGCCTTGGACATGTGTCAAATGTTATTCAACTCAAGATCCTTTGCATACTGTTATTGGGATGCCGGCTTTGTCTCCTACAATGGCTCAAGGTAACATTGCAAAGTGGTTTAAGAAAGAAGGAGACAAGATTGAACCGGGCGACATACTATGTGAAATTGAAACTGACAAAGCTACCGTTGAATTTGAAAGTCTTGAAGAAGGGTTTCTAGCCAAGATACTGGTACCTGAAGGTTCAAAAGATGTGCCAGTGGGACAGCCTATTGCAATAACGGTTGAGGATGCAGATGATATCCAAAACCTCCCTGCTAATGTGGTCAGTGGCTCTGAGGTCAAAGAGGATATACCAGCACCTCAGAATGTCAAGAATGAAGAGGGGGTACAAGATACAAGTTCCGTGGGCATAAACACATCAGAACTTCCTCCTCACATTGTCGTTGAAATGCCAGCTTTATCACCGACAATGAGCCAGGGAAACATTTTTAAGTGGAGAAAGAAAGAAGGAGATAAGATAGAAGTGGGTGATATAATATGTGAGATAGAGACAGACAAAGCAACCATCGAATATGAATTTCTTGAAGAGGGATACTTGGCTAAGATACTAGCACCAGAAGGTACAAAGGACATAGCTGTTGGCCAACCAATTGCAGTAACAGTTGAGGATGCAGCTGATCTGGAAACTGTAAAGAATGCTGTTGGCAGTGGTTCAGCTGTTAAAGAAGAAAAACCAGTCCTTCAGGATACTAGAGATGTGACTAGATCAGAAAAAACCAGTATTTTAAGGATCAGTCCAGCAGCAAAGTTGCTTGTCACAGAACATGGATTGGATACATCATCGCTGCAGGCATCAGGTGCTCATGGTACATTGCTGAAAGGGGATGTTTTAGCTGCAATCAAGTCAGGAATAGGATCTTCAAAAGTTTCATCTAAAGAAAAGACAACTCCATCGTTGTTAAAGGCGTCAAGTACTCATGCTCCAATATCAGCAGACTCAAAGCCTTCAAAACAGACAGATTCTTTTGAAGATTTTCCCAATAGTCAAATCCGCAAGGTGATAGCTACTAGATTGCAAGAATCAAAACAAAACATACCGCACTTATATTTATCATCAGATGTTCTTCTGGACCCTCTTCTTTCTCTTAGAAAGGATCTTATAGAACAGCATAATGTTAAAGTTTCAGTTAATGACATTGTCATCAAAGCTGTAGCTGTTGCATTGAGAAATGTACCTGAAGCAAATGCTTACTGGGATGCTGAAAAAGGAGAAGTCTTTCTTTGTGATTCCATTGATATATCAATTGCTGTGGCCACTGAGAAGGGCCTGATGACTCCAATTGTGAAGAATGCAGATCAGAAATCAATATCAGCAATCTCTGCAGAGGTTAAGGAACTAGCTGAAAAGGCACGAGCGGGAAAGCTTAAACCAAATGAATTCCAAGGAGGAACTTTCAGCATTTCAAACTTGGGGATGTTTCCAGTGGATACTTTCTGTGCAATTATAAACCCACCTCAGGCCAGTATCCTTGCTGTTGGCAGAGGCAACAAAGTCGTTGAACCAGTAATAGGAAGCGACGGAATTGAGAGGCCTGCAGTTGTCACCAAAATGAACTTGACATTATCGGCTGATCATCGAGTTTTTGATGGCAAAGTTGGAGGTTCGTTCCTCTCGGCTTTACGTTCAAACTTCAGTGATATCCGAAGGCTTCTTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0045254 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

637

Amino Acids

68.35

Weight (kDa)

5.53

Isoelectric Point (pI)

45.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	84 - 155	4.2e-19	Biotin-requiring enzyme
Biotin_lipoyl	PF00364	212 - 283	2.7e-16	Biotin-requiring enzyme
E3_binding	PF02817	327 - 361	4.3e-07	e3 binding domain
2-oxoacid_dh	PF00198	406 - 635	3.1e-74	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52200 AT3G52200
fragaria_vesca	FvH4_7g18300 FvH4_7g18300 FvH4_7g18300
malus_domestica	MD01G1099700.v1.1 MD07G1166600.v1.1
prunus_persica	Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1
pyrus_communis	pycom01g12680 pycom07g16180
rosa_chinensis	RchiOBHm_Chr1g0361921
rosa_laevigata	RLG00000027686
rosa_multiflora	Rmu_sc0008041.1_g000008
rosa_roxburghii	Rroxscaffold_4G00294620
rosa_rugosa	Rorug01G0292400
rosa_samantha	Rh1AG302900 Rh1CG284300 Rh1DG296100
rosa_wichuraiana	Rw1G026840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1697
Acc65I	GGTACC	1 cut(s)	421
AccB1I	GGYRCC	1 cut(s)	421
AccBSI	CCGCTC	1 cut(s)	1607
AccII	CGCG	3 cut(s)	13, 39, 41
AciI	CCGC	5 cut(s)	13, 37, 1243, 1289, 1607
AclWI	GGATC	4 cut(s)	230, 989, 1114, 1353
AcoI	YGGCCR	2 cut(s)	832, 1503
AcsI	RAATTY	4 cut(s)	112, 380, 767, 1628
AcuI	CTGAAG	5 cut(s)	218, 447, 917, 1449, 1867
AcyI	GRCGYC	1 cut(s)	1157
AdeI	CACNNNGTG	1 cut(s)	28
AfaI	GTAC	6 cut(s)	423, 579, 814, 1060, 1165, 1425
AfiI	CCNNNNNNNGG	2 cut(s)	1708, 1760
AflIII	ACRYGT	1 cut(s)	209
AjnI	CCWGG	1 cut(s)	666
Alw21I	GWGCWC	2 cut(s)	61, 1054
Alw26I	GTCTC	3 cut(s)	276, 315, 734
AlwI	GGATC	4 cut(s)	230, 989, 1114, 1353
AoxI	GGCC	6 cut(s)	146, 832, 1503, 1516, 1710, 1781
ApeKI	GCWGC	4 cut(s)	863, 992, 1036, 1085
ApoI	RAATTY	4 cut(s)	112, 380, 767, 1628
Asp700I	GAANNNNTTC	3 cut(s)	402, 1224, 1467
Asp718I	GGTACC	1 cut(s)	421
AspLEI	GCGC	4 cut(s)	7, 41, 43, 124
AspS9I	GGNCC	2 cut(s)	1321, 1516
AsuC2I	CCSGG	1 cut(s)	336
AsuHPI	GGTGA	5 cut(s)	140, 645, 731, 1261, 1786
AvaII	GGWCC	1 cut(s)	1321
AxyI	CCTNAGG	1 cut(s)	1707
BalI	TGGCCA	2 cut(s)	834, 1505
BanI	GGYRCC	1 cut(s)	421
BanII	GRGCYC	1 cut(s)	61
BarI	GAAGNNNNNNTAC	2 cut(s)	561, 593
BauI	CACGAG	1 cut(s)	1602
Bbv12I	GWGCWC	2 cut(s)	61, 1054
BbvI	GCAGC	4 cut(s)	875, 1004, 1023, 1072
BccI	CCATC	5 cut(s)	52, 196, 764, 1150, 1832
BcgI	CGANNNNNNTGC	2 cut(s)	1726, 1760
BciT130I	CCWGG	1 cut(s)	668
BciVI	GTATCC	5 cut(s)	776, 931, 1018, 1672, 1727
BclI	TGATCA	1 cut(s)	1822
BcnI	CCSGG	1 cut(s)	336
BcoDI	GTCTC	3 cut(s)	276, 315, 734
BfaI	CTAG	6 cut(s)	407, 800, 942, 954, 1260, 1589
BfmI	CTRYAG	4 cut(s)	1037, 1401, 1572, 1785
BfoI	RGCGCY	1 cut(s)	8
BfuI	GTATCC	5 cut(s)	776, 931, 1018, 1672, 1727
BisI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
BlsI	GCNGC	5 cut(s)	15, 865, 994, 1038, 1087
BmcAI	AGTACT	1 cut(s)	1165
Bme1390I	CCNGG	2 cut(s)	336, 668
Bme18I	GGWCC	1 cut(s)	1321
BmgT120I	GGNCC	2 cut(s)	1321, 1516
BmiI	GGNNCC	2 cut(s)	423, 1323
BmrFI	CCNGG	2 cut(s)	336, 668
BmrI	ACTGGG	2 cut(s)	20, 1453
BmsI	GCATC	6 cut(s)	161, 249, 469, 850, 1052, 1438
BmuI	ACTGGG	2 cut(s)	20, 1453
BoxI	GACNNNNGTC	1 cut(s)	212
BpmI	CTGGAG	1 cut(s)	192
BpuEI	CTTGAG	2 cut(s)	216, 270
BpuMI	CCSGG	1 cut(s)	336
BsaHI	GRCGYC	1 cut(s)	1157
BsaJI	CCNNGG	4 cut(s)	203, 594, 667, 1633
BsaXI	ACNNNNNCTCC	2 cut(s)	1156, 1186
Bsc4I	CCNNNNNNNGG	2 cut(s)	1708, 1760
Bse118I	RCCGGY	1 cut(s)	262
Bse21I	CCTNAGG	1 cut(s)	1707
Bse3DI	GCAATG	2 cut(s)	294, 1061
BseBI	CCWGG	1 cut(s)	668
BseDI	CCNNGG	4 cut(s)	203, 594, 667, 1633
BseGI	GGATG	6 cut(s)	264, 484, 865, 1081, 1453, 1671
BseLI	CCNNNNNNNGG	2 cut(s)	1708, 1760
BseMI	GCAATG	2 cut(s)	294, 1061
BseMII	CTCAG	5 cut(s)	44, 516, 566, 1500, 1721
BsePI	GCGCGC	1 cut(s)	39
BseRI	GAGGAG	2 cut(s)	61, 612
BseXI	GCAGC	4 cut(s)	875, 1004, 1023, 1072
Bsh1236I	CGCG	3 cut(s)	13, 39, 41
BshFI	GGCC	6 cut(s)	148, 834, 1505, 1518, 1712, 1783
BshNI	GGYRCC	1 cut(s)	421
BsiHKAI	GWGCWC	2 cut(s)	61, 1054
BsiSI	CCGG	2 cut(s)	263, 335
BslFI	GGGAC	1 cut(s)	465
BslI	CCNNNNNNNGG	2 cut(s)	1708, 1760
BsmAI	GTCTC	3 cut(s)	276, 315, 734
BsmFI	GGGAC	1 cut(s)	465
BsmI	GAATGC	2 cut(s)	892, 1546
BsnI	GGCC	6 cut(s)	148, 834, 1505, 1518, 1712, 1783
Bsp1286I	GDGCHC	2 cut(s)	61, 1054
BspACI	CCGC	5 cut(s)	13, 37, 1243, 1289, 1607
BspANI	GGCC	6 cut(s)	148, 834, 1505, 1518, 1712, 1783
BspCNI	CTCAG	5 cut(s)	45, 517, 565, 1501, 1720
BspFNI	CGCG	3 cut(s)	13, 39, 41
BspHI	TCATGA	1 cut(s)	178
BspLI	GGNNCC	2 cut(s)	423, 1323
BspMAI	CTGCAG	3 cut(s)	1041, 1576, 1789
BspPI	GGATC	4 cut(s)	230, 989, 1114, 1353
BspT107I	GGYRCC	1 cut(s)	421
BsrBI	CCGCTC	1 cut(s)	1607
BsrDI	GCAATG	2 cut(s)	294, 1061
BsrFI	RCCGGY	1 cut(s)	262
BssAI	RCCGGY	1 cut(s)	262
BssECI	CCNNGG	4 cut(s)	203, 594, 667, 1633
BssHII	GCGCGC	1 cut(s)	39
BssNI	GRCGYC	1 cut(s)	1157
BssSI	CACGAG	1 cut(s)	1602
BssT1I	CCWWGG	2 cut(s)	203, 1633
Bst2BI	CACGAG	1 cut(s)	1602
Bst2UI	CCWGG	1 cut(s)	668
Bst4CI	ACNGT	5 cut(s)	250, 376, 472, 853, 880
Bst6I	CTCTTC	3 cut(s)	564, 771, 1332
BstACI	GRCGYC	1 cut(s)	1157
BstAPI	GCANNNNNTGC	2 cut(s)	1049, 1571
BstC8I	GCNNGC	7 cut(s)	41, 61, 150, 264, 645, 1041, 1785
BstDEI	CTNAG	7 cut(s)	53, 525, 552, 792, 1337, 1509, 1707
BstDSI	CCRYGG	1 cut(s)	594
BstF5I	GGATG	6 cut(s)	264, 484, 865, 1081, 1453, 1671
BstFNI	CGCG	3 cut(s)	13, 39, 41
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	4 cut(s)	7, 41, 43, 124
BstMAI	GTCTC	3 cut(s)	276, 315, 734
BstMWI	GCNNNNNNNGC	5 cut(s)	13, 145, 1049, 1571, 1598
BstNI	CCWGG	1 cut(s)	668
BstNSI	RCATGY	1 cut(s)	213
BstPAI	GACNNNNGTC	1 cut(s)	212
BstSCI	CCNGG	2 cut(s)	334, 666
BstSFI	CTRYAG	4 cut(s)	1037, 1401, 1572, 1785
BstUI	CGCG	3 cut(s)	13, 39, 41
BstV1I	GCAGC	4 cut(s)	875, 1004, 1023, 1072
BstX2I	RGATCY	3 cut(s)	235, 1106, 1345
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	3 cut(s)	235, 1106, 1345
Bsu36I	CCTNAGG	1 cut(s)	1707
BsuI	GTATCC	5 cut(s)	776, 931, 1018, 1672, 1727
BsuRI	GGCC	6 cut(s)	148, 834, 1505, 1518, 1712, 1783
BtgI	CCRYGG	1 cut(s)	594
BtgZI	GCGATG	1 cut(s)	1017
BtsCI	GGATG	6 cut(s)	264, 484, 865, 1081, 1453, 1671
BtsI	GCAGTG	1 cut(s)	904
BtsIMutI	CAGTG	8 cut(s)	33, 168, 453, 523, 904, 1506, 1680, 1891
Cac8I	GCNNGC	7 cut(s)	41, 61, 150, 264, 645, 1041, 1785
CciI	TCATGA	1 cut(s)	178
CfoI	GCGC	4 cut(s)	7, 41, 43, 124
Cfr10I	RCCGGY	1 cut(s)	262
Cfr13I	GGNCC	2 cut(s)	1321, 1516
CseI	GACGC	1 cut(s)	1146
Csp6I	GTAC	6 cut(s)	422, 578, 813, 1059, 1164, 1424
CspCI	CAANNNNNGTGG	4 cut(s)	576, 611, 1482, 1517
CviAII	CATG	6 cut(s)	163, 179, 210, 1016, 1055, 1169
CviQI	GTAC	6 cut(s)	422, 578, 813, 1059, 1164, 1424
DdeI	CTNAG	7 cut(s)	53, 525, 552, 792, 1337, 1509, 1707
DraIII	CACNNNGTG	1 cut(s)	28
EaeI	YGGCCR	2 cut(s)	832, 1503
Eam1104I	CTCTTC	3 cut(s)	564, 771, 1332
EarI	CTCTTC	3 cut(s)	564, 771, 1332
Ecl136II	GAGCTC	1 cut(s)	59
Eco130I	CCWWGG	2 cut(s)	203, 1633
Eco147I	AGGCCT	1 cut(s)	1783
Eco24I	GRGCYC	1 cut(s)	61
Eco32I	GATATC	2 cut(s)	490, 1891
Eco47I	GGWCC	1 cut(s)	1321
Eco53kI	GAGCTC	1 cut(s)	59
Eco57I	CTGAAG	5 cut(s)	218, 447, 917, 1449, 1867
Eco81I	CCTNAGG	1 cut(s)	1707
EcoICRI	GAGCTC	1 cut(s)	59
EcoO109I	RGGNCCY	1 cut(s)	1516
EcoRI	GAATTC	1 cut(s)	1628
EcoRII	CCWGG	1 cut(s)	666
EcoRV	GATATC	2 cut(s)	490, 1891
EcoT14I	CCWWGG	2 cut(s)	203, 1633
EcoT38I	GRGCYC	1 cut(s)	61
ErhI	CCWWGG	2 cut(s)	203, 1633
FaeI	CATG	6 cut(s)	166, 182, 213, 1019, 1058, 1172
FalI	AAGNNNNNCTT	2 cut(s)	1455, 1487
FaqI	GGGAC	1 cut(s)	465
FatI	CATG	6 cut(s)	162, 178, 209, 1015, 1054, 1168
FauI	CCCGC	2 cut(s)	44, 1600
FbaI	TGATCA	1 cut(s)	1822
Fnu4HI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
FokI	GGATG	6 cut(s)	271, 491, 872, 1088, 1460, 1678
FriOI	GRGCYC	1 cut(s)	61
Fsp4HI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
FspBI	CTAG	6 cut(s)	407, 800, 942, 954, 1260, 1589
GlaI	GCGC	4 cut(s)	6, 40, 42, 123
GluI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
GsuI	CTGGAG	1 cut(s)	192
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	6 cut(s)	148, 834, 1505, 1518, 1712, 1783
HapII	CCGG	2 cut(s)	263, 335
HgaI	GACGC	1 cut(s)	1146
HhaI	GCGC	4 cut(s)	7, 41, 43, 124
Hin1I	GRCGYC	1 cut(s)	1157
Hin1II	CATG	6 cut(s)	166, 182, 213, 1019, 1058, 1172
Hin6I	GCGC	4 cut(s)	5, 39, 41, 122
HinP1I	GCGC	4 cut(s)	5, 39, 41, 122
HindIII	AAGCTT	1 cut(s)	1613
HinfI	GANTC	5 cut(s)	1187, 1211, 1271, 1478, 1525
HpaII	CCGG	2 cut(s)	263, 335
HphI	GGTGA	5 cut(s)	140, 645, 731, 1261, 1786
Hpy166II	GTNNAC	1 cut(s)	78
Hpy188I	TCNGA	7 cut(s)	526, 555, 613, 961, 1309, 1551, 1895
Hpy8I	GTNNAC	1 cut(s)	78
Hpy99I	CGWCG	1 cut(s)	1772
HpyCH4III	ACNGT	5 cut(s)	250, 376, 472, 853, 880
HpyCH4IV	ACGT	1 cut(s)	1873
HpyF10VI	GCNNNNNNNGC	5 cut(s)	13, 145, 1049, 1571, 1598
HpyF3I	CTNAG	7 cut(s)	53, 525, 552, 792, 1337, 1509, 1707
HpySE526I	ACGT	1 cut(s)	1873
Hsp92I	GRCGYC	1 cut(s)	1157
Hsp92II	CATG	6 cut(s)	166, 182, 213, 1019, 1058, 1172
HspAI	GCGC	4 cut(s)	5, 39, 41, 122
KpnI	GGTACC	1 cut(s)	425
KroI	GCCGGC	1 cut(s)	262
KroNI	GCCGGC	1 cut(s)	264
Ksp22I	TGATCA	1 cut(s)	1822
LmnI	GCTCC	1 cut(s)	1177
Lsp1109I	GCAGC	4 cut(s)	875, 1004, 1023, 1072
LweI	GCATC	6 cut(s)	161, 249, 469, 850, 1052, 1438
MaeI	CTAG	6 cut(s)	407, 800, 942, 954, 1260, 1589
MaeII	ACGT	1 cut(s)	1873
MaeIII	GTNAC	5 cut(s)	290, 847, 949, 1006, 1792
MbiI	CCGCTC	1 cut(s)	1607
MboII	GAAGA	9 cut(s)	407, 581, 788, 929, 1101, 1232, 1307, 1319, 1549
MfeI	CAATTG	3 cut(s)	840, 1494, 1530
MflI	RGATCY	3 cut(s)	235, 1106, 1345
MhlI	GDGCHC	2 cut(s)	61, 1054
MlsI	TGGCCA	2 cut(s)	834, 1505
MluNI	TGGCCA	2 cut(s)	834, 1505
MlyI	GAGTC	2 cut(s)	1181, 1519
MmeI	TCCRAC	1 cut(s)	1828
Mox20I	TGGCCA	2 cut(s)	834, 1505
MroNI	GCCGGC	1 cut(s)	262
MroXI	GAANNNNTTC	3 cut(s)	402, 1224, 1467
MscI	TGGCCA	2 cut(s)	834, 1505
MslI	CAYNNNNRTG	1 cut(s)	1799
Msp20I	TGGCCA	2 cut(s)	834, 1505
MspA1I	CMGCKG	2 cut(s)	866, 908
MspI	CCGG	2 cut(s)	263, 335
MspR9I	CCNGG	2 cut(s)	336, 668
MunI	CAATTG	3 cut(s)	840, 1494, 1530
Mva1269I	GAATGC	2 cut(s)	892, 1546
MvaI	CCWGG	1 cut(s)	668
MvnI	CGCG	3 cut(s)	13, 39, 41
MwoI	GCNNNNNNNGC	5 cut(s)	13, 145, 1049, 1571, 1598
NaeI	GCCGGC	1 cut(s)	264
NciI	CCSGG	1 cut(s)	336
NgoMIV	GCCGGC	1 cut(s)	262
NlaIII	CATG	6 cut(s)	166, 182, 213, 1019, 1058, 1172
NlaIV	GGNNCC	2 cut(s)	423, 1323
NmeAIII	GCCGAG	1 cut(s)	1844
NmuCI	GTSAC	3 cut(s)	949, 1006, 1792
NspI	RCATGY	1 cut(s)	213
PagI	TCATGA	1 cut(s)	178
PauI	GCGCGC	1 cut(s)	39
PceI	AGGCCT	1 cut(s)	1783
PciI	ACATGT	1 cut(s)	209
PctI	GAATGC	2 cut(s)	892, 1546
PdiI	GCCGGC	1 cut(s)	264
PdmI	GAANNNNTTC	3 cut(s)	402, 1224, 1467
PfeI	GAWTC	3 cut(s)	1211, 1271, 1478
PflFI	GACNNNGTC	1 cut(s)	1388
PkrI	GCNGC	5 cut(s)	15, 865, 994, 1038, 1087
PleI	GAGTC	2 cut(s)	1181, 1519
PpsI	GAGTC	2 cut(s)	1181, 1519
PscI	ACATGT	1 cut(s)	209
PshAI	GACNNNNGTC	1 cut(s)	212
PsiI	TTATAA	1 cut(s)	1697
Psp124BI	GAGCTC	1 cut(s)	61
Psp6I	CCWGG	1 cut(s)	666
PspGI	CCWGG	1 cut(s)	666
PspN4I	GGNNCC	2 cut(s)	423, 1323
PspPI	GGNCC	2 cut(s)	1321, 1516
PstI	CTGCAG	3 cut(s)	1041, 1576, 1789
PsuI	RGATCY	3 cut(s)	235, 1106, 1345
PsyI	GACNNNGTC	1 cut(s)	1388
PteI	GCGCGC	1 cut(s)	39
PvuII	CAGCTG	2 cut(s)	866, 908
RsaI	GTAC	6 cut(s)	423, 579, 814, 1060, 1165, 1425
RsaNI	GTAC	6 cut(s)	422, 578, 813, 1059, 1164, 1424
RseI	CAYNNNNRTG	1 cut(s)	1799
SacI	GAGCTC	1 cut(s)	61
SatI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
Sau96I	GGNCC	2 cut(s)	1321, 1516
ScaI	AGTACT	1 cut(s)	1165
SchI	GAGTC	2 cut(s)	1181, 1519
ScrFI	CCNGG	2 cut(s)	336, 668
SduI	GDGCHC	2 cut(s)	61, 1054
SfaNI	GCATC	6 cut(s)	161, 249, 469, 850, 1052, 1438
SfcI	CTRYAG	4 cut(s)	1037, 1401, 1572, 1785
SinI	GGWCC	1 cut(s)	1321
SmiMI	CAYNNNNRTG	1 cut(s)	1799
SmlI	CTYRAG	2 cut(s)	231, 285
SmoI	CTYRAG	2 cut(s)	231, 285
SseBI	AGGCCT	1 cut(s)	1783
SsiI	CCGC	5 cut(s)	13, 37, 1243, 1289, 1607
SspMI	CTAG	6 cut(s)	407, 800, 942, 954, 1260, 1589
SstI	GAGCTC	1 cut(s)	61
StuI	AGGCCT	1 cut(s)	1783
StyD4I	CCNGG	2 cut(s)	334, 666
StyI	CCWWGG	2 cut(s)	203, 1633
TaaI	ACNGT	5 cut(s)	250, 376, 472, 853, 880
TaiI	ACGT	1 cut(s)	1876
TaqI	TCGA	4 cut(s)	19, 94, 759, 1828
TatI	WGTACW	1 cut(s)	1163
TauI	GCSGC	1 cut(s)	16
TfiI	GAWTC	3 cut(s)	1211, 1271, 1478
TscAI	CASTG	8 cut(s)	33, 175, 453, 523, 904, 1513, 1680, 1891
TseFI	GTSAC	3 cut(s)	949, 1006, 1792
TseI	GCWGC	4 cut(s)	863, 992, 1036, 1085
Tsp45I	GTSAC	3 cut(s)	949, 1006, 1792
TspDTI	ATGAA	6 cut(s)	167, 582, 780, 1110, 1641, 1817
TspGWI	ACGGA	2 cut(s)	583, 1785
TspRI	CASTG	8 cut(s)	33, 175, 453, 523, 904, 1513, 1680, 1891
Tth111I	GACNNNGTC	1 cut(s)	1388
VpaK11BI	GGWCC	1 cut(s)	1321
XapI	RAATTY	4 cut(s)	112, 380, 767, 1628
XceI	RCATGY	1 cut(s)	213
XmnI	GAANNNNTTC	3 cut(s)	402, 1224, 1467
XspI	CTAG	6 cut(s)	407, 800, 942, 954, 1260, 1589
ZrmI	AGTACT	1 cut(s)	1165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.