Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0009625

Physical Location & Seq

Genomic Coordinates Reverse (-)

13338031 .. 13344719

Gene Structure 6689 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g12680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGGCGCTCTCGCGGCTTCGACACCCAGTGATCTCCCGCGCTCCATCTCTCCTGAGAGCTCGCCTTCTCTCCTCGTCCACCAGATCACTGACTCGAACTTTCAGTTTGCAAAATTCTACTGCACTTGGTGATAATACGCTGTTAAGGCCAGCATCTTTGTCCATGTTCACTGGAGTTCATGATATACCATCAAAGCTGAAGCCTTGGACATGTGTCAAATATTACTCAACTCAAGATCCTTTGCATACTGTTATTGGGATGCCAGCTTTGTCTCCTACAATGGCTCAAGGTAACATTGCAAAGTGGTTTAAGAAAGAAGGAGACAAGATTGAACCGGGCGACATACTCTGTGAAATTGAAACTGACAAAGCTACCGTTGAATTTGAAAGTCTTGAAGAAGGGTTTCTAGCCAAGATACTGGTACCTGAAGGTTCAAAAGATGTGCCAGTGGGACAGCCTATTGCAATAACGGTTGAGGATGCAGATGATATCCAAAACCTCCCTGCTAATGTGGTCAGTGGCTCTGAGGTCAAAGAGGATATACCAGTACCTCAGAATGTCAAGAATGAAGAGGGGGTACAAGATACAAGTTCCATGGGCATAAACACATCAGAACTTCCTCCTCACATTGTCGTTGAAATGCCAGCTTTATCACCAACAATGAGCCAGGGAAACATTTTTAAGTGGAGAAAGAAAGAAGGAGATAAGATAGAAGTGGGTGATATAATATGTGAGATAGAGACAGACAAAGCAACCATTGAATATGAATTTCTTGAAGAGGGATACTTGGCTAAGATACTAGCACCAGAAGGTACAAAGGACATAGCTGTTGGCCAACCAATTGCAGTAACAGTTGAGGATGCAGCTGATCTGGAAACTGTAAAGAATGCTGTTGGCAGTGGTTCAACTGTTAAAGAAGAAAAACCAGTCCGTCAGGATACTAGAGATGTGACTAGATCAGAAAAAACCAGTATTTTAAGGATCAGTCCAGCAGCAAAGTTGCTTGTCACAGAACATGGATTGGATACATCATCGCTGCAGGCATCAGGTGCTCATGGTACATTGCTGAAAGGGGATGTTTTAGCTGCAATCAAGTCAGGAATAGGATCTTTAAAAGTTTCATCTAAAGAAAAGACAACTCCATCGTTGTTAAAGGCGTCAAGTACTCATGCCCCAATATCAGCACAGTCAAAGCCTTCAAAACAGACAGATTCTTTTGAAGATTTTCCCAATAGTCAAATCCGCAGGGTGATAGCTACTAGATTGCAAGAATCAAAACAAAACATACCGCACTTATATTTATCATCAGATGTTCTTCTGGACCCTCTTCTTTCTCTTAGGAAGGATCTTATAGAACAGCATAATGTTAAAGTTTCAGTTAATGACATTGTCGTCAAAGCTGTAGCTGTTGCACTGAGAAATGTACCTGAAGCAAATGCTTACTGGGATGCTGAAAAAGGAGAAGTCTTTCTTTGTGATTCCATTGATATATCAATTGCTGTGGCCACTGAGAAGGGCCTGATGACTCCAATTGTGAAGAATGCAGATCGAGAAATCAATATCAGCAATCTCTGCAGAGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0045254 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

528

Amino Acids

57.12

Weight (kDa)

5.37

Isoelectric Point (pI)

50.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	84 - 155	3.2e-19	Biotin-requiring enzyme
Biotin_lipoyl	PF00364	212 - 283	2.1e-16	Biotin-requiring enzyme
E3_binding	PF02817	327 - 361	3.4e-07	e3 binding domain
2-oxoacid_dh	PF00198	406 - 517	1.1e-32	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52200 AT3G52200
fragaria_vesca	FvH4_7g18300 FvH4_7g18300 FvH4_7g18300
malus_domestica	MD01G1099700.v1.1 MD07G1166600.v1.1
prunus_persica	Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1
pyrus_communis	pycom01g12680 pycom07g16180
rosa_chinensis	RchiOBHm_Chr1g0361921
rosa_laevigata	RLG00000027686
rosa_multiflora	Rmu_sc0008041.1_g000008
rosa_roxburghii	Rroxscaffold_4G00294620
rosa_rugosa	Rorug01G0292400
rosa_samantha	Rh1AG302900 Rh1CG284300 Rh1DG296100
rosa_wichuraiana	Rw1G026840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1391
Acc65I	GGTACC	1 cut(s)	421
AccB1I	GGYRCC	1 cut(s)	421
AccII	CGCG	2 cut(s)	13, 39
AciI	CCGC	4 cut(s)	13, 37, 1243, 1289
AclWI	GGATC	4 cut(s)	230, 989, 1114, 1353
AcoI	YGGCCR	2 cut(s)	832, 1503
AcsI	RAATTY	3 cut(s)	112, 380, 767
AcuI	CTGAAG	3 cut(s)	218, 447, 1449
AcyI	GRCGYC	1 cut(s)	1157
AdeI	CACNNNGTG	2 cut(s)	28, 128
AfaI	GTAC	7 cut(s)	423, 549, 579, 814, 1060, 1165, 1425
AflIII	ACRYGT	1 cut(s)	209
AjnI	CCWGG	1 cut(s)	666
Alw21I	GWGCWC	2 cut(s)	61, 1054
Alw26I	GTCTC	3 cut(s)	276, 315, 734
AlwI	GGATC	4 cut(s)	230, 989, 1114, 1353
AoxI	GGCC	4 cut(s)	146, 832, 1503, 1516
ApeKI	GCWGC	4 cut(s)	863, 992, 1036, 1085
ApoI	RAATTY	3 cut(s)	112, 380, 767
Asp700I	GAANNNNTTC	3 cut(s)	402, 1224, 1467
Asp718I	GGTACC	1 cut(s)	421
AspLEI	GCGC	2 cut(s)	7, 41
AspS9I	GGNCC	2 cut(s)	1321, 1516
AsuC2I	CCSGG	1 cut(s)	336
AsuHPI	GGTGA	4 cut(s)	140, 645, 731, 1261
AvaII	GGWCC	1 cut(s)	1321
BalI	TGGCCA	2 cut(s)	834, 1505
BanI	GGYRCC	1 cut(s)	421
BanII	GRGCYC	1 cut(s)	61
BarI	GAAGNNNNNNTAC	2 cut(s)	561, 593
Bbv12I	GWGCWC	2 cut(s)	61, 1054
BbvI	GCAGC	4 cut(s)	875, 1004, 1023, 1072
BccI	CCATC	3 cut(s)	52, 196, 1150
BciT130I	CCWGG	1 cut(s)	668
BciVI	GTATCC	3 cut(s)	776, 931, 1018
BcnI	CCSGG	1 cut(s)	336
BcoDI	GTCTC	3 cut(s)	276, 315, 734
BfaI	CTAG	5 cut(s)	407, 800, 942, 954, 1260
BfmI	CTRYAG	3 cut(s)	1037, 1401, 1573
BfoI	RGCGCY	1 cut(s)	8
BfuI	GTATCC	3 cut(s)	776, 931, 1018
BisI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
BlsI	GCNGC	5 cut(s)	15, 865, 994, 1038, 1087
BmcAI	AGTACT	1 cut(s)	1165
Bme1390I	CCNGG	2 cut(s)	336, 668
Bme18I	GGWCC	1 cut(s)	1321
BmgT120I	GGNCC	2 cut(s)	1321, 1516
BmiI	GGNNCC	2 cut(s)	423, 1323
BmrFI	CCNGG	2 cut(s)	336, 668
BmrI	ACTGGG	2 cut(s)	20, 1453
BmsI	GCATC	6 cut(s)	161, 249, 469, 850, 1052, 1438
BmuI	ACTGGG	2 cut(s)	20, 1453
BoxI	GACNNNNGTC	1 cut(s)	212
BpmI	CTGGAG	1 cut(s)	192
BpuEI	CTTGAG	2 cut(s)	216, 270
BpuMI	CCSGG	1 cut(s)	336
BsaHI	GRCGYC	1 cut(s)	1157
BsaJI	CCNNGG	3 cut(s)	203, 594, 667
Bse1I	ACTGG	8 cut(s)	26, 175, 423, 446, 545, 926, 969, 1448
Bse3DI	GCAATG	2 cut(s)	294, 1061
BseBI	CCWGG	1 cut(s)	668
BseDI	CCNNGG	3 cut(s)	203, 594, 667
BseGI	GGATG	5 cut(s)	264, 484, 865, 1081, 1453
BseMI	GCAATG	2 cut(s)	294, 1061
BseMII	CTCAG	5 cut(s)	44, 516, 566, 1406, 1500
BseNI	ACTGG	8 cut(s)	26, 175, 423, 446, 545, 926, 969, 1448
BseRI	GAGGAG	2 cut(s)	61, 612
BseXI	GCAGC	4 cut(s)	875, 1004, 1023, 1072
BsgI	GTGCAG	1 cut(s)	105
Bsh1236I	CGCG	2 cut(s)	13, 39
BshFI	GGCC	4 cut(s)	148, 834, 1505, 1518
BshNI	GGYRCC	1 cut(s)	421
BsiHKAI	GWGCWC	2 cut(s)	61, 1054
BsiSI	CCGG	1 cut(s)	335
BslFI	GGGAC	1 cut(s)	465
BsmAI	GTCTC	3 cut(s)	276, 315, 734
BsmFI	GGGAC	1 cut(s)	465
BsmI	GAATGC	2 cut(s)	892, 1546
BsnI	GGCC	4 cut(s)	148, 834, 1505, 1518
Bsp1286I	GDGCHC	2 cut(s)	61, 1054
Bsp143I	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
Bsp19I	CCATGG	1 cut(s)	594
BspACI	CCGC	4 cut(s)	13, 37, 1243, 1289
BspANI	GGCC	4 cut(s)	148, 834, 1505, 1518
BspCNI	CTCAG	5 cut(s)	45, 517, 565, 1407, 1501
BspFNI	CGCG	2 cut(s)	13, 39
BspHI	TCATGA	1 cut(s)	178
BspLI	GGNNCC	2 cut(s)	423, 1323
BspMAI	CTGCAG	2 cut(s)	1041, 1577
BspPI	GGATC	4 cut(s)	230, 989, 1114, 1353
BspT107I	GGYRCC	1 cut(s)	421
BsrDI	GCAATG	2 cut(s)	294, 1061
BsrI	ACTGG	8 cut(s)	26, 175, 423, 446, 545, 926, 969, 1448
BssECI	CCNNGG	3 cut(s)	203, 594, 667
BssMI	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
BssNI	GRCGYC	1 cut(s)	1157
BssT1I	CCWWGG	2 cut(s)	203, 594
Bst2UI	CCWGG	1 cut(s)	668
Bst4CI	ACNGT	7 cut(s)	250, 376, 472, 853, 880, 910, 1188
Bst6I	CTCTTC	3 cut(s)	564, 771, 1332
BstACI	GRCGYC	1 cut(s)	1157
BstAPI	GCANNNNNTGC	2 cut(s)	1049, 1572
BstC8I	GCNNGC	5 cut(s)	61, 150, 264, 645, 1041
BstDEI	CTNAG	7 cut(s)	53, 525, 552, 792, 1337, 1415, 1509
BstDSI	CCRYGG	1 cut(s)	594
BstF5I	GGATG	5 cut(s)	264, 484, 865, 1081, 1453
BstFNI	CGCG	2 cut(s)	13, 39
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 41
BstKTI	GATC	9 cut(s)	33, 86, 238, 871, 959, 984, 1109, 1348, 1549
BstMAI	GTCTC	3 cut(s)	276, 315, 734
BstMBI	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
BstMWI	GCNNNNNNNGC	4 cut(s)	13, 145, 1049, 1572
BstNI	CCWGG	1 cut(s)	668
BstNSI	RCATGY	1 cut(s)	213
BstPAI	GACNNNNGTC	1 cut(s)	212
BstSCI	CCNGG	2 cut(s)	334, 666
BstSFI	CTRYAG	3 cut(s)	1037, 1401, 1573
BstUI	CGCG	2 cut(s)	13, 39
BstV1I	GCAGC	4 cut(s)	875, 1004, 1023, 1072
BstX2I	RGATCY	3 cut(s)	235, 1106, 1345
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	3 cut(s)	235, 1106, 1345
BsuI	GTATCC	3 cut(s)	776, 931, 1018
BsuRI	GGCC	4 cut(s)	148, 834, 1505, 1518
BtgI	CCRYGG	1 cut(s)	594
BtgZI	GCGATG	1 cut(s)	1017
BtsCI	GGATG	5 cut(s)	264, 484, 865, 1081, 1453
BtsI	GCAGTG	1 cut(s)	904
BtsIMutI	CAGTG	8 cut(s)	33, 86, 168, 453, 523, 904, 1412, 1506
Cac8I	GCNNGC	5 cut(s)	61, 150, 264, 645, 1041
CciI	TCATGA	1 cut(s)	178
CfoI	GCGC	2 cut(s)	7, 41
Cfr13I	GGNCC	2 cut(s)	1321, 1516
CseI	GACGC	1 cut(s)	1146
Csp6I	GTAC	7 cut(s)	422, 548, 578, 813, 1059, 1164, 1424
CspCI	CAANNNNNGTGG	2 cut(s)	1482, 1517
CviAII	CATG	7 cut(s)	163, 179, 210, 595, 1016, 1055, 1169
CviQI	GTAC	7 cut(s)	422, 548, 578, 813, 1059, 1164, 1424
DdeI	CTNAG	7 cut(s)	53, 525, 552, 792, 1337, 1415, 1509
DpnI	GATC	9 cut(s)	32, 85, 237, 870, 958, 983, 1108, 1347, 1548
DpnII	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
DraI	TTTAAA	1 cut(s)	1113
DraIII	CACNNNGTG	2 cut(s)	28, 128
DrdI	GACNNNNNNGTC	1 cut(s)	1391
DseDI	GACNNNNNNGTC	1 cut(s)	1391
EaeI	YGGCCR	2 cut(s)	832, 1503
Eam1104I	CTCTTC	3 cut(s)	564, 771, 1332
EarI	CTCTTC	3 cut(s)	564, 771, 1332
Ecl136II	GAGCTC	1 cut(s)	59
Eco130I	CCWWGG	2 cut(s)	203, 594
Eco24I	GRGCYC	1 cut(s)	61
Eco32I	GATATC	1 cut(s)	490
Eco47I	GGWCC	1 cut(s)	1321
Eco53kI	GAGCTC	1 cut(s)	59
Eco57I	CTGAAG	3 cut(s)	218, 447, 1449
EcoICRI	GAGCTC	1 cut(s)	59
EcoO109I	RGGNCCY	1 cut(s)	1516
EcoRII	CCWGG	1 cut(s)	666
EcoRV	GATATC	1 cut(s)	490
EcoT14I	CCWWGG	2 cut(s)	203, 594
EcoT38I	GRGCYC	1 cut(s)	61
ErhI	CCWWGG	2 cut(s)	203, 594
FaeI	CATG	7 cut(s)	166, 182, 213, 598, 1019, 1058, 1172
FalI	AAGNNNNNCTT	2 cut(s)	1455, 1487
FaqI	GGGAC	1 cut(s)	465
FatI	CATG	7 cut(s)	162, 178, 209, 594, 1015, 1054, 1168
FauI	CCCGC	1 cut(s)	44
Fnu4HI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
FokI	GGATG	5 cut(s)	271, 491, 872, 1088, 1460
FriOI	GRGCYC	1 cut(s)	61
Fsp4HI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
FspBI	CTAG	5 cut(s)	407, 800, 942, 954, 1260
GlaI	GCGC	2 cut(s)	6, 40
GluI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
GsuI	CTGGAG	1 cut(s)	192
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	4 cut(s)	148, 834, 1505, 1518
HapII	CCGG	1 cut(s)	335
HgaI	GACGC	1 cut(s)	1146
HhaI	GCGC	2 cut(s)	7, 41
Hin1I	GRCGYC	1 cut(s)	1157
Hin1II	CATG	7 cut(s)	166, 182, 213, 598, 1019, 1058, 1172
Hin6I	GCGC	2 cut(s)	5, 39
HinP1I	GCGC	2 cut(s)	5, 39
HinfI	GANTC	5 cut(s)	91, 1211, 1271, 1478, 1525
HpaII	CCGG	1 cut(s)	335
HphI	GGTGA	4 cut(s)	140, 645, 731, 1261
Hpy166II	GTNNAC	2 cut(s)	78, 168
Hpy188I	TCNGA	5 cut(s)	526, 555, 613, 961, 1309
Hpy8I	GTNNAC	2 cut(s)	78, 168
HpyAV	CCTTC	9 cut(s)	74, 311, 392, 422, 692, 803, 1206, 1336, 1507
HpyCH4III	ACNGT	7 cut(s)	250, 376, 472, 853, 880, 910, 1188
HpyF10VI	GCNNNNNNNGC	4 cut(s)	13, 145, 1049, 1572
HpyF3I	CTNAG	7 cut(s)	53, 525, 552, 792, 1337, 1415, 1509
Hsp92I	GRCGYC	1 cut(s)	1157
Hsp92II	CATG	7 cut(s)	166, 182, 213, 598, 1019, 1058, 1172
HspAI	GCGC	2 cut(s)	5, 39
KpnI	GGTACC	1 cut(s)	425
Kzo9I	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
LmnI	GCTCC	1 cut(s)	46
Lsp1109I	GCAGC	4 cut(s)	875, 1004, 1023, 1072
LweI	GCATC	6 cut(s)	161, 249, 469, 850, 1052, 1438
MaeI	CTAG	5 cut(s)	407, 800, 942, 954, 1260
MaeIII	GTNAC	4 cut(s)	290, 847, 949, 1006
MalI	GATC	9 cut(s)	32, 85, 237, 870, 958, 983, 1108, 1347, 1548
MboI	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
MboII	GAAGA	8 cut(s)	407, 581, 788, 929, 1232, 1307, 1319, 1549
MfeI	CAATTG	3 cut(s)	840, 1494, 1530
MflI	RGATCY	3 cut(s)	235, 1106, 1345
MhlI	GDGCHC	2 cut(s)	61, 1054
MlsI	TGGCCA	2 cut(s)	834, 1505
MluCI	AATT	7 cut(s)	112, 354, 380, 767, 840, 1494, 1530
MluNI	TGGCCA	2 cut(s)	834, 1505
MlyI	GAGTC	2 cut(s)	85, 1519
Mox20I	TGGCCA	2 cut(s)	834, 1505
MroXI	GAANNNNTTC	3 cut(s)	402, 1224, 1467
MscI	TGGCCA	2 cut(s)	834, 1505
Msp20I	TGGCCA	2 cut(s)	834, 1505
MspA1I	CMGCKG	1 cut(s)	866
MspI	CCGG	1 cut(s)	335
MspR9I	CCNGG	2 cut(s)	336, 668
MunI	CAATTG	3 cut(s)	840, 1494, 1530
Mva1269I	GAATGC	2 cut(s)	892, 1546
MvaI	CCWGG	1 cut(s)	668
MvnI	CGCG	2 cut(s)	13, 39
MwoI	GCNNNNNNNGC	4 cut(s)	13, 145, 1049, 1572
NciI	CCSGG	1 cut(s)	336
NcoI	CCATGG	1 cut(s)	594
NdeII	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
NlaIII	CATG	7 cut(s)	166, 182, 213, 598, 1019, 1058, 1172
NlaIV	GGNNCC	2 cut(s)	423, 1323
NmuCI	GTSAC	2 cut(s)	949, 1006
NspI	RCATGY	1 cut(s)	213
PagI	TCATGA	1 cut(s)	178
PciI	ACATGT	1 cut(s)	209
PctI	GAATGC	2 cut(s)	892, 1546
PdmI	GAANNNNTTC	3 cut(s)	402, 1224, 1467
PfeI	GAWTC	3 cut(s)	1211, 1271, 1478
PflFI	GACNNNGTC	1 cut(s)	1388
PkrI	GCNGC	5 cut(s)	15, 865, 994, 1038, 1087
PleI	GAGTC	2 cut(s)	85, 1519
PpsI	GAGTC	2 cut(s)	85, 1519
PscI	ACATGT	1 cut(s)	209
PshAI	GACNNNNGTC	1 cut(s)	212
Psp124BI	GAGCTC	1 cut(s)	61
Psp6I	CCWGG	1 cut(s)	666
PspGI	CCWGG	1 cut(s)	666
PspN4I	GGNNCC	2 cut(s)	423, 1323
PspPI	GGNCC	2 cut(s)	1321, 1516
PstI	CTGCAG	2 cut(s)	1041, 1577
PsuI	RGATCY	3 cut(s)	235, 1106, 1345
PsyI	GACNNNGTC	1 cut(s)	1388
PvuII	CAGCTG	1 cut(s)	866
RsaI	GTAC	7 cut(s)	423, 549, 579, 814, 1060, 1165, 1425
RsaNI	GTAC	7 cut(s)	422, 548, 578, 813, 1059, 1164, 1424
SacI	GAGCTC	1 cut(s)	61
SatI	GCNGC	5 cut(s)	14, 864, 993, 1037, 1086
Sau3AI	GATC	9 cut(s)	30, 83, 235, 868, 956, 981, 1106, 1345, 1546
Sau96I	GGNCC	2 cut(s)	1321, 1516
ScaI	AGTACT	1 cut(s)	1165
SchI	GAGTC	2 cut(s)	85, 1519
ScrFI	CCNGG	2 cut(s)	336, 668
SduI	GDGCHC	2 cut(s)	61, 1054
SfaNI	GCATC	6 cut(s)	161, 249, 469, 850, 1052, 1438
SfcI	CTRYAG	3 cut(s)	1037, 1401, 1573
SinI	GGWCC	1 cut(s)	1321
SmlI	CTYRAG	2 cut(s)	231, 285
SmoI	CTYRAG	2 cut(s)	231, 285
Sse9I	AATT	7 cut(s)	112, 354, 380, 767, 840, 1494, 1530
SsiI	CCGC	4 cut(s)	13, 37, 1243, 1289
SspI	AATATT	1 cut(s)	221
SspMI	CTAG	5 cut(s)	407, 800, 942, 954, 1260
SstI	GAGCTC	1 cut(s)	61
StyD4I	CCNGG	2 cut(s)	334, 666
StyI	CCWWGG	2 cut(s)	203, 594
TaaI	ACNGT	7 cut(s)	250, 376, 472, 853, 880, 910, 1188
TaqI	TCGA	3 cut(s)	19, 94, 1549
TasI	AATT	7 cut(s)	112, 354, 380, 767, 840, 1494, 1530
TatI	WGTACW	1 cut(s)	1163
TauI	GCSGC	1 cut(s)	16
TfiI	GAWTC	3 cut(s)	1211, 1271, 1478
TscAI	CASTG	8 cut(s)	33, 93, 175, 453, 523, 904, 1419, 1513
TseFI	GTSAC	2 cut(s)	949, 1006
TseI	GCWGC	4 cut(s)	863, 992, 1036, 1085
Tsp45I	GTSAC	2 cut(s)	949, 1006
TspDTI	ATGAA	4 cut(s)	167, 582, 780, 1110
TspGWI	ACGGA	1 cut(s)	920
TspRI	CASTG	8 cut(s)	33, 93, 175, 453, 523, 904, 1419, 1513
Tth111I	GACNNNGTC	1 cut(s)	1388
VpaK11BI	GGWCC	1 cut(s)	1321
XapI	RAATTY	3 cut(s)	112, 380, 767
XceI	RCATGY	1 cut(s)	213
XmnI	GAANNNNTTC	3 cut(s)	402, 1224, 1467
XspI	CTAG	5 cut(s)	407, 800, 942, 954, 1260
ZrmI	AGTACT	1 cut(s)	1165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.