Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0009625

Physical Location & Seq

Genomic Coordinates Reverse (-)

14650086 .. 14657389

Gene Structure 7304 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00294620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGGCGCTCTCACGGCTTCGACACCCTGTGATCTCACGCGCCCCTTCTCTTTTCCGAGCTCGCCTTCTCTCCTCGTCCACCAGATCACTCACTCGAAGTTCCAGTGTGCAGAATTTGACTGTGGGAGGTGACAGTGCTCTTTTAAGGCCTGCATCTTTGTCCATGCTCACCGGAGTCCAGTATAAGTTTTCACACCTCAAGCCTTGGACTGGTGTCAAGCATTACTCAACTGCCGATCCTTTGTATGCTGTCCTTGATATGCCTGCTTTGTCTCCTACAATGAGTCAAGGTAACATTGCTAAATGGAGGAAGAAAGAGGGAGATAAGATTGAAGTGGGTGATGTACTATGTGAAATTGAAACTGACAAAGCTACTCTTGAATTTGAAAGTCTTGAAGAAGGGTTTCTAGCCAAGATACTGGTACCTGAAGGTTCAAAGGATGTGCCTGTGGGACAACCTATTGCAGTAACGGTCGAGGATAAAGATGAAATCCAAAACATCCCTGCTAATATTGTGGGTGGATCTGAGGTGAAAGAGGACATACCACAACATCAGAAAGATGAGGCACAAGATACAAGTTCTGTAGGGATAAACACAGCAGACCTTCCTCCTCACATTGTTGTTGAAATGCCAGCTTTGTCTCCAACAATGAGCCAAGGAAACATTGCTGTGTGGAGAAAGAAAGAAGGAGATAAGATAGAAGTTGGTGATGTGTTATGTGAGATAGAGACGGACAAAGCCACCCTTGAATTTGAATGCCTTGAAGAGGGATATTTGGCTAAAATACTAGCACCTGAAGGTTCAAAGGATGTGGCTGTTGGCCAACCAATTGCAGTAACAGTTGAGGATGCAGCTGATTTGGAAACTGTAAAGAATGTAAAAACAACCAGTGTCAAAAGGATCAGTCCAGCAGCTAAGAATCTAATTCTAGAACACGGATTGGATACATTGTCATTGAGGGCATCAGGTGCTCATGGTACGCTACTGAAAGGGGATGTTTTAGCGGCAATCAAGTCAGGAACTGGATCTTCAAAAGTTTCATCATCTAAAGAGAAGGCACCTTCATCTTCTCAAGCACATATAAAGATAGCTCCAGCATCACCAGAGTCAAGGTCTTTACAGCAGACAGATTTTGAAGAGTTTCCCAACAGTCAAATTCGCAAGGTGATAGCTAGTAGGTTGCTGGAATCAAAACAAAACATACCACACTTGTATTTATCCTCAGATGTTATCCTGGATCCTCTTCTTTCTCTTAGAAAGAATCTTAAAGAGCAGCATAATGTTAAAGTTTCAGTGAATGACATTGTCATTAGAGCTGTAGCTATTGCACTGAGAAATGTACCAGAAGCAAATGCTTACTGGGATGCTGAAAAGGAAGAAGTGATTCTTTGTGATTCTGTTGATATATCAATTGCTGTCGCTACCGACAAGGGTCTGATGACTCCAATTGTGAAAAATGCGGATCAGAAAACAATATCAGCAATCTCCTCAGAGGTCAAGGAACTAGCTGAGAAGGCACGGGCAGGAAAGCTTAAGCCAAGTGAATTCCAAGGAGGGACTTTCAGGCCAGTATCCTTGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0045254 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

527

Amino Acids

56.88

Weight (kDa)

5.73

Isoelectric Point (pI)

53.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	85 - 156	1.6e-18	Biotin-requiring enzyme
Biotin_lipoyl	PF00364	208 - 279	3.9e-17	Biotin-requiring enzyme
E3_binding	PF02817	300 - 334	3.2e-06	e3 binding domain
2-oxoacid_dh	PF00198	377 - 521	3.1e-49	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52200 AT3G52200
fragaria_vesca	FvH4_7g18300 FvH4_7g18300 FvH4_7g18300
malus_domestica	MD01G1099700.v1.1 MD07G1166600.v1.1
prunus_persica	Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1
pyrus_communis	pycom01g12680 pycom07g16180
rosa_chinensis	RchiOBHm_Chr1g0361921
rosa_laevigata	RLG00000027686
rosa_multiflora	Rmu_sc0008041.1_g000008
rosa_roxburghii	Rroxscaffold_4G00294620
rosa_rugosa	Rorug01G0292400
rosa_samantha	Rh1AG302900 Rh1CG284300 Rh1DG296100
rosa_wichuraiana	Rw1G026840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	421
AccB1I	GGYRCC	2 cut(s)	421, 1057
AccII	CGCG	1 cut(s)	39
AciI	CCGC	2 cut(s)	1004, 1460
AclWI	GGATC	7 cut(s)	230, 529, 908, 1033, 1232, 1245, 1470
AcoI	YGGCCR	1 cut(s)	820
AcsI	RAATTY	5 cut(s)	112, 380, 749, 1155, 1544
AcuI	CTGAAG	2 cut(s)	447, 816
AdeI	CACNNNGTG	1 cut(s)	28
AfaI	GTAC	4 cut(s)	345, 423, 979, 1341
AfiI	CCNNNNNNNGG	2 cut(s)	209, 766
AflII	CTTAAG	1 cut(s)	1532
AjnI	CCWGG	1 cut(s)	1233
Alw21I	GWGCWC	3 cut(s)	61, 139, 973
Alw26I	GTCTC	3 cut(s)	276, 645, 722
AlwI	GGATC	7 cut(s)	230, 529, 908, 1033, 1232, 1245, 1470
AlwNI	CAGNNNCTG	1 cut(s)	1022
AoxI	GGCC	3 cut(s)	146, 820, 1565
ApeKI	GCWGC	3 cut(s)	851, 911, 1273
ApoI	RAATTY	5 cut(s)	112, 380, 749, 1155, 1544
Asp700I	GAANNNNTTC	3 cut(s)	402, 1140, 1383
Asp718I	GGTACC	1 cut(s)	421
AspLEI	GCGC	2 cut(s)	7, 41
AsuHPI	GGTGA	7 cut(s)	140, 160, 350, 541, 719, 1092, 1177
BalI	TGGCCA	1 cut(s)	822
BamHI	GGATCC	1 cut(s)	1237
BanI	GGYRCC	2 cut(s)	421, 1057
BanII	GRGCYC	1 cut(s)	61
Bbv12I	GWGCWC	3 cut(s)	61, 139, 973
BbvI	GCAGC	3 cut(s)	863, 923, 1285
BceAI	ACGGC	1 cut(s)	29
BciT130I	CCWGG	1 cut(s)	1235
BciVI	GTATCC	2 cut(s)	937, 1582
BcoDI	GTCTC	3 cut(s)	276, 645, 722
BfaI	CTAG	5 cut(s)	407, 788, 929, 1173, 1505
BfmI	CTRYAG	3 cut(s)	582, 1317, 1580
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	1532
BfuI	GTATCC	2 cut(s)	937, 1582
BisI	GCNGC	4 cut(s)	852, 912, 1005, 1274
BlsI	GCNGC	4 cut(s)	853, 913, 1006, 1275
Bme1390I	CCNGG	1 cut(s)	1235
BmiI	GGNNCC	3 cut(s)	423, 1059, 1239
BmrFI	CCNGG	1 cut(s)	1235
BmrI	ACTGGG	1 cut(s)	1369
BmsI	GCATC	5 cut(s)	161, 838, 971, 1106, 1354
BmuI	ACTGGG	1 cut(s)	1369
BoxI	GACNNNNGTC	2 cut(s)	212, 1431
BpmI	CTGGAG	1 cut(s)	1077
BpuEI	CTTGAG	2 cut(s)	182, 1056
BsaJI	CCNNGG	3 cut(s)	203, 655, 1549
BsaWI	WCCGGW	1 cut(s)	170
Bsc4I	CCNNNNNNNGG	2 cut(s)	209, 766
Bse1I	ACTGG	8 cut(s)	102, 178, 214, 423, 888, 1027, 1364, 1568
Bse3DI	GCAATG	2 cut(s)	294, 663
BseBI	CCWGG	1 cut(s)	1235
BseDI	CCNNGG	3 cut(s)	203, 655, 1549
BseGI	GGATG	6 cut(s)	445, 498, 814, 853, 1000, 1369
BseLI	CCNNNNNNNGG	2 cut(s)	209, 766
BseMI	GCAATG	2 cut(s)	294, 663
BseMII	CTCAG	5 cut(s)	516, 1236, 1322, 1500, 1503
BseNI	ACTGG	8 cut(s)	102, 178, 214, 423, 888, 1027, 1364, 1568
BseRI	GAGGAG	3 cut(s)	61, 600, 1477
BseXI	GCAGC	3 cut(s)	863, 923, 1285
BsgI	GTGCAG	1 cut(s)	128
Bsh1236I	CGCG	1 cut(s)	39
Bsh1285I	CGRYCG	1 cut(s)	474
BshFI	GGCC	3 cut(s)	148, 822, 1567
BshNI	GGYRCC	2 cut(s)	421, 1057
BsiEI	CGRYCG	1 cut(s)	474
BsiHKAI	GWGCWC	3 cut(s)	61, 139, 973
BsiSI	CCGG	1 cut(s)	171
BslFI	GGGAC	2 cut(s)	465, 1570
BslI	CCNNNNNNNGG	2 cut(s)	209, 766
BsmAI	GTCTC	3 cut(s)	276, 645, 722
BsmBI	CGTCTC	1 cut(s)	722
BsmFI	GGGAC	2 cut(s)	465, 1570
BsmI	GAATGC	1 cut(s)	761
BsnI	GGCC	3 cut(s)	148, 822, 1567
Bsp1286I	GDGCHC	3 cut(s)	61, 139, 973
Bsp143I	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
BspACI	CCGC	2 cut(s)	1004, 1460
BspANI	GGCC	3 cut(s)	148, 822, 1567
BspCNI	CTCAG	5 cut(s)	517, 1235, 1323, 1501, 1502
BspFNI	CGCG	1 cut(s)	39
BspLI	GGNNCC	3 cut(s)	423, 1059, 1239
BspPI	GGATC	7 cut(s)	230, 529, 908, 1033, 1232, 1245, 1470
BspT107I	GGYRCC	2 cut(s)	421, 1057
BspTI	CTTAAG	1 cut(s)	1532
BsrDI	GCAATG	2 cut(s)	294, 663
BsrI	ACTGG	8 cut(s)	102, 178, 214, 423, 888, 1027, 1364, 1568
BssECI	CCNNGG	3 cut(s)	203, 655, 1549
BssMI	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
BssT1I	CCWWGG	3 cut(s)	203, 655, 1549
Bst2UI	CCWGG	1 cut(s)	1235
Bst4CI	ACNGT	6 cut(s)	121, 134, 472, 841, 868, 1151
Bst6I	CTCTTC	3 cut(s)	759, 1131, 1248
BstAFI	CTTAAG	1 cut(s)	1532
BstAPI	GCANNNNNTGC	1 cut(s)	968
BstC8I	GCNNGC	4 cut(s)	61, 150, 264, 633
BstDEI	CTNAG	7 cut(s)	525, 915, 1222, 1253, 1331, 1489, 1509
BstENI	CCTNNNNNAGG	1 cut(s)	764
BstF5I	GGATG	6 cut(s)	445, 498, 814, 853, 1000, 1369
BstFNI	CGCG	1 cut(s)	39
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 41
BstKTI	GATC	8 cut(s)	33, 86, 238, 524, 903, 1028, 1240, 1465
BstMAI	GTCTC	3 cut(s)	276, 645, 722
BstMBI	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
BstMCI	CGRYCG	1 cut(s)	474
BstMWI	GCNNNNNNNGC	3 cut(s)	13, 968, 1514
BstNI	CCWGG	1 cut(s)	1235
BstPAI	GACNNNNGTC	2 cut(s)	212, 1431
BstSCI	CCNGG	1 cut(s)	1233
BstSFI	CTRYAG	3 cut(s)	582, 1317, 1580
BstUI	CGCG	1 cut(s)	39
BstV1I	GCAGC	3 cut(s)	863, 923, 1285
BstX2I	RGATCY	3 cut(s)	521, 1025, 1237
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	3 cut(s)	521, 1025, 1237
BsuI	GTATCC	2 cut(s)	937, 1582
BsuRI	GGCC	3 cut(s)	148, 822, 1567
BtsCI	GGATG	6 cut(s)	445, 498, 814, 853, 1000, 1369
BtsIMutI	CAGTG	5 cut(s)	109, 139, 895, 1299, 1328
Cac8I	GCNNGC	4 cut(s)	61, 150, 264, 633
CaiI	CAGNNNCTG	1 cut(s)	1022
CfoI	GCGC	2 cut(s)	7, 41
Csp6I	GTAC	4 cut(s)	344, 422, 978, 1340
CspCI	CAANNNNNGTGG	2 cut(s)	792, 827
CviAII	CATG	2 cut(s)	163, 974
CviQI	GTAC	4 cut(s)	344, 422, 978, 1340
DdeI	CTNAG	7 cut(s)	525, 915, 1222, 1253, 1331, 1489, 1509
DpnI	GATC	8 cut(s)	32, 85, 237, 523, 902, 1027, 1239, 1464
DpnII	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
DraIII	CACNNNGTG	1 cut(s)	28
EaeI	YGGCCR	1 cut(s)	820
Eam1104I	CTCTTC	3 cut(s)	759, 1131, 1248
EarI	CTCTTC	3 cut(s)	759, 1131, 1248
Ecl136II	GAGCTC	1 cut(s)	59
Eco130I	CCWWGG	3 cut(s)	203, 655, 1549
Eco147I	AGGCCT	1 cut(s)	148
Eco24I	GRGCYC	1 cut(s)	61
Eco53kI	GAGCTC	1 cut(s)	59
Eco57I	CTGAAG	2 cut(s)	447, 816
EcoICRI	GAGCTC	1 cut(s)	59
EcoNI	CCTNNNNNAGG	1 cut(s)	764
EcoRI	GAATTC	1 cut(s)	1544
EcoRII	CCWGG	1 cut(s)	1233
EcoT14I	CCWWGG	3 cut(s)	203, 655, 1549
EcoT38I	GRGCYC	1 cut(s)	61
ErhI	CCWWGG	3 cut(s)	203, 655, 1549
Esp3I	CGTCTC	1 cut(s)	722
FaeI	CATG	2 cut(s)	166, 977
FalI	AAGNNNNNCTT	6 cut(s)	360, 392, 729, 761, 1371, 1403
FaqI	GGGAC	2 cut(s)	465, 1570
FatI	CATG	2 cut(s)	162, 973
Fnu4HI	GCNGC	4 cut(s)	852, 912, 1005, 1274
FokI	GGATG	6 cut(s)	452, 485, 821, 860, 1007, 1376
FriOI	GRGCYC	1 cut(s)	61
Fsp4HI	GCNGC	4 cut(s)	852, 912, 1005, 1274
FspBI	CTAG	5 cut(s)	407, 788, 929, 1173, 1505
GlaI	GCGC	2 cut(s)	6, 40
GluI	GCNGC	4 cut(s)	852, 912, 1005, 1274
GsuI	CTGGAG	1 cut(s)	1077
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	3 cut(s)	148, 822, 1567
HapII	CCGG	1 cut(s)	171
HhaI	GCGC	2 cut(s)	7, 41
Hin1II	CATG	2 cut(s)	166, 977
Hin6I	GCGC	2 cut(s)	5, 39
HinP1I	GCGC	2 cut(s)	5, 39
HindIII	AAGCTT	1 cut(s)	1529
HinfI	GANTC	9 cut(s)	174, 283, 919, 1106, 1187, 1261, 1384, 1394, 1441
HpaII	CCGG	1 cut(s)	171
HphI	GGTGA	7 cut(s)	140, 160, 350, 541, 719, 1092, 1177
Hpy166II	GTNNAC	1 cut(s)	78
Hpy188I	TCNGA	7 cut(s)	56, 526, 555, 1225, 1437, 1467, 1492
Hpy188III	TCNNGA	4 cut(s)	377, 392, 929, 1017
Hpy8I	GTNNAC	1 cut(s)	78
HpyCH4III	ACNGT	6 cut(s)	121, 134, 472, 841, 868, 1151
HpyCH4V	TGCA	6 cut(s)	109, 152, 464, 833, 851, 1328
HpyF10VI	GCNNNNNNNGC	3 cut(s)	13, 968, 1514
HpyF3I	CTNAG	7 cut(s)	525, 915, 1222, 1253, 1331, 1489, 1509
Hsp92II	CATG	2 cut(s)	166, 977
HspAI	GCGC	2 cut(s)	5, 39
KpnI	GGTACC	1 cut(s)	425
Kzo9I	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
LmnI	GCTCC	1 cut(s)	1096
Lsp1109I	GCAGC	3 cut(s)	863, 923, 1285
LweI	GCATC	5 cut(s)	161, 838, 971, 1106, 1354
MaeI	CTAG	5 cut(s)	407, 788, 929, 1173, 1505
MaeIII	GTNAC	4 cut(s)	128, 290, 466, 835
MalI	GATC	8 cut(s)	32, 85, 237, 523, 902, 1027, 1239, 1464
MboI	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
MboII	GAAGA	8 cut(s)	322, 407, 776, 1020, 1059, 1148, 1235, 1388
MfeI	CAATTG	3 cut(s)	828, 1410, 1446
MflI	RGATCY	3 cut(s)	521, 1025, 1237
MhlI	GDGCHC	3 cut(s)	61, 139, 973
MlsI	TGGCCA	1 cut(s)	822
MluNI	TGGCCA	1 cut(s)	822
MlyI	GAGTC	4 cut(s)	183, 292, 1115, 1435
MmeI	TCCRAC	1 cut(s)	668
Mox20I	TGGCCA	1 cut(s)	822
MroXI	GAANNNNTTC	3 cut(s)	402, 1140, 1383
MscI	TGGCCA	1 cut(s)	822
MseI	TTAA	4 cut(s)	143, 1266, 1284, 1533
MslI	CAYNNNNRTG	1 cut(s)	668
Msp20I	TGGCCA	1 cut(s)	822
MspA1I	CMGCKG	1 cut(s)	854
MspCI	CTTAAG	1 cut(s)	1532
MspI	CCGG	1 cut(s)	171
MspR9I	CCNGG	1 cut(s)	1235
MunI	CAATTG	3 cut(s)	828, 1410, 1446
Mva1269I	GAATGC	1 cut(s)	761
MvaI	CCWGG	1 cut(s)	1235
MvnI	CGCG	1 cut(s)	39
MwoI	GCNNNNNNNGC	3 cut(s)	13, 968, 1514
NdeII	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
NlaIII	CATG	2 cut(s)	166, 977
NlaIV	GGNNCC	3 cut(s)	423, 1059, 1239
NmuCI	GTSAC	1 cut(s)	128
PceI	AGGCCT	1 cut(s)	148
PctI	GAATGC	1 cut(s)	761
PdmI	GAANNNNTTC	3 cut(s)	402, 1140, 1383
PfeI	GAWTC	5 cut(s)	919, 1187, 1261, 1384, 1394
PflFI	GACNNNGTC	1 cut(s)	1304
PfoI	TCCNGGA	1 cut(s)	1233
PkrI	GCNGC	4 cut(s)	853, 913, 1006, 1275
PleI	GAGTC	4 cut(s)	182, 291, 1114, 1435
PpsI	GAGTC	4 cut(s)	182, 291, 1114, 1435
PshAI	GACNNNNGTC	2 cut(s)	212, 1431
Psp124BI	GAGCTC	1 cut(s)	61
Psp6I	CCWGG	1 cut(s)	1233
PspGI	CCWGG	1 cut(s)	1233
PspN4I	GGNNCC	3 cut(s)	423, 1059, 1239
PstNI	CAGNNNCTG	1 cut(s)	1022
PsuI	RGATCY	3 cut(s)	521, 1025, 1237
PsyI	GACNNNGTC	1 cut(s)	1304
PvuII	CAGCTG	1 cut(s)	854
RsaI	GTAC	4 cut(s)	345, 423, 979, 1341
RsaNI	GTAC	4 cut(s)	344, 422, 978, 1340
RseI	CAYNNNNRTG	1 cut(s)	668
SacI	GAGCTC	1 cut(s)	61
SaqAI	TTAA	4 cut(s)	143, 1266, 1284, 1533
SatI	GCNGC	4 cut(s)	852, 912, 1005, 1274
Sau3AI	GATC	8 cut(s)	30, 83, 235, 521, 900, 1025, 1237, 1462
SchI	GAGTC	4 cut(s)	183, 292, 1115, 1435
ScrFI	CCNGG	1 cut(s)	1235
SduI	GDGCHC	3 cut(s)	61, 139, 973
SfaNI	GCATC	5 cut(s)	161, 838, 971, 1106, 1354
SfcI	CTRYAG	3 cut(s)	582, 1317, 1580
SmiMI	CAYNNNNRTG	1 cut(s)	668
SmlI	CTYRAG	3 cut(s)	197, 1071, 1532
SmoI	CTYRAG	3 cut(s)	197, 1071, 1532
SseBI	AGGCCT	1 cut(s)	148
SsiI	CCGC	2 cut(s)	1004, 1460
SspI	AATATT	1 cut(s)	511
SspMI	CTAG	5 cut(s)	407, 788, 929, 1173, 1505
SstI	GAGCTC	1 cut(s)	61
StuI	AGGCCT	1 cut(s)	148
StyD4I	CCNGG	1 cut(s)	1233
StyI	CCWWGG	3 cut(s)	203, 655, 1549
TaaI	ACNGT	6 cut(s)	121, 134, 472, 841, 868, 1151
TaqI	TCGA	3 cut(s)	19, 94, 474
TatI	WGTACW	1 cut(s)	343
TauI	GCSGC	1 cut(s)	1007
TfiI	GAWTC	5 cut(s)	919, 1187, 1261, 1384, 1394
Tru1I	TTAA	4 cut(s)	143, 1266, 1284, 1533
Tru9I	TTAA	4 cut(s)	143, 1266, 1284, 1533
TscAI	CASTG	5 cut(s)	109, 139, 895, 1299, 1335
TseFI	GTSAC	1 cut(s)	128
TseI	GCWGC	3 cut(s)	851, 911, 1273
Tsp45I	GTSAC	1 cut(s)	128
TspDTI	ATGAA	3 cut(s)	501, 1029, 1053
TspGWI	ACGGA	2 cut(s)	746, 951
TspRI	CASTG	5 cut(s)	109, 139, 895, 1299, 1335
Tth111I	GACNNNGTC	1 cut(s)	1304
Vha464I	CTTAAG	1 cut(s)	1532
XagI	CCTNNNNNAGG	1 cut(s)	764
XapI	RAATTY	5 cut(s)	112, 380, 749, 1155, 1544
XbaI	TCTAGA	1 cut(s)	928
XmnI	GAANNNNTTC	3 cut(s)	402, 1140, 1383
XspI	CTAG	5 cut(s)	407, 788, 929, 1173, 1505

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.