MD07G1166600.v1.1

of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009625
Physical Location & Seq
Reverse (-)
24075353 .. 24083670
8318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1166600.v1.1.491

Sequence Viewer

Length: 1911 bp
ATGGCGCTCTCACGGCTTCGCCACCCAGTGATCTCCCGTGCGCCGTCTCTCCTGAGAGCTCGACTTCTCTCCTCGTCCACCAGATCACTCACTCGTACTTTCAGTGTGCAAAATTCTGCTGAGCTTGGTGATGATACGCCGTTAAGGCCAGCAACTTTGTCCATGCTCACTGGAGTTCATGATAAACCAACAAAGCTGAAGCCTTGGACGGGTGTCAAGTATTATTCAACTCAAGATCCTTTGCATACTGTCATCGGGATGCCGGCGTTGTCTCCTACAATGACTCAAGGTAACATTGCAAAGTGGTGGAAGAAGGAAGGAGACAAGATTGAGCCAGGCGACATACTATGTGAAATTGAAACTGACAAAGCTACTATTGAATTTGAAAGTCTTGAAGAAGGGTTTCTAGCCAAGATATTGGCACCTGACGGTTCAAAAGATGTGCCTGTGGGACAGCCTATTGCAATAACGGTTGAGGATGCAGATGATATCCAAAACCTCCCTGCTAATGTGGTCGGTGGATCTGAGGTCAAAGAGGATATACGAGCACATCAGAATGTCAGAAAGGAAGAGGAGGTACAAGATACAAGTTCTGTGGGCATAAACACATCAGAACTTCCTCCTCATATTGTCATTGAAATGCCAGCTTTATCACCAACAATGAGCCAGGGAAATATTTTTAAGTGGACAAAGAAAGAAGGAGATAAGATAGAAGTGGGTGATATAATATGTGAGATAGAGACAGACAAAGCAACCATTGAATTTGAATGTCTTGAAGAGGGATACTTGGCTAAGATACTAGCGCCAGACGGTACAAAGGATATAGCTGTTGGCCAACCAATTGCAGTAACAGTTGAGGATGCATCTGATCTGGAAACTGTAAAGAATGCTGTTAGCAGTGGTTCACCTGTCAAAGAAGAAACACCAGTCCGTCATGATACTAGAGATGTAACTAGATCAGAAAAAACCAGTATTTCAAGGATCAGTCCAGCAGCAAAGTTGCTTATTACAGAACATGGATTGGATACATCATTGCTGAAGGCATCAGGTCCTCATGGTACATTGCTGAAAGGGGATGTTTTAGCTGCAATCAAGTCAGGAATAGGGTGTTCAAAAGTTTCATCCAAAGAAAAGACTACTCCATCACCGTCAAAGGCATCAGGTGCTCATGCCCCGAAACCATCAGAGTCAAAGCCTTCAAAACAGACAGATTCTTTTGAAGATTTTCCCAATAGTCAAATCCGCAAGGTGATAGCTACTAGACTGCTAGAATCAAAACAAAACATACCGCATTTATTTTTATCATCAGATGTTATACTGGACCCTCTTCTTTCTCTTAGAAGGGATCTTATAGAACAGCATAATGTTAAAGTTTCAGTTAATGACATTGTTATCAAAGCTGTAGCTGTTGCACTGAAAAATGTACCGGAGGCAAATGCTTACTGGGATGCTGAAAAAGGAGAAGTCTTTCTTTGTGATTCTGTTGATATATCAATTGCTGTGGCCACTGAGAAGGGCCTGATGACTCCAATTGTGAAGAATGCAGATCAGAAATCAATATCAGCAATCTCTGCAGAGGTCAAGGAGTTAGCTGAGAAGGCACGAACGGGAAAGCTTAAGCCAAATGAATTCCAAGGAGGAACTTTCAGCATTTCAAACTTGGGGATGTTTCCAGTGGATCATTTCTGTGCAATTATAAACCCACCTCAGGCCAGTATCCTTGCTGTTGGCAGAGGCAACAAAGTCGTTGAACCAGTGATCGGAAGCGACGGAATTGAGAGGCCGGCAGTTGTCACCAAAATGAACTTGACATTATCGGCTGATCATCGAGTTTTTGACGGAAAAGTTGGAGGTTCGTTCCTCTCAGCTTTACGTTCAAACTTCAGTGATATCCGAAGGCTTCTTTTGTAA

Protein Analysis

637

Amino Acids

68.64

Weight (kDa)

5.76

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 84 - 155 6.7e-18 Biotin-requiring enzyme
Biotin_lipoyl PF00364 212 - 283 2.5e-17 Biotin-requiring enzyme
E3_binding PF02817 327 - 361 3.8e-08 e3 binding domain
2-oxoacid_dh PF00198 406 - 635 3e-73 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1697
AccB1I GGYRCC 1 cut(s) 421
AciI CCGC 2 cut(s) 1243, 1289
AclWI GGATC 5 cut(s) 230, 529, 989, 1353, 1686
AcoI YGGCCR 2 cut(s) 832, 1503
AcsI RAATTY 4 cut(s) 112, 380, 761, 1628
AcuI CTGAAG 3 cut(s) 218, 1058, 1867
AdeI CACNNNGTG 1 cut(s) 28
AfaI GTAC 5 cut(s) 97, 579, 814, 1060, 1425
AfiI CCNNNNNNNGG 3 cut(s) 209, 1708, 1760
AflII CTTAAG 1 cut(s) 1616
AjnI CCWGG 2 cut(s) 334, 666
Alw21I GWGCWC 3 cut(s) 61, 550, 1168
Alw26I GTCTC 4 cut(s) 51, 276, 315, 734
AlwI GGATC 5 cut(s) 230, 529, 989, 1353, 1686
AoxI GGCC 6 cut(s) 146, 832, 1503, 1516, 1710, 1781
ApeKI GCWGC 2 cut(s) 992, 1085
ApoI RAATTY 4 cut(s) 112, 380, 761, 1628
Asp700I GAANNNNTTC 3 cut(s) 402, 1224, 1467
AspLEI GCGC 3 cut(s) 7, 43, 805
AspS9I GGNCC 3 cut(s) 1049, 1321, 1516
AsuHPI GGTGA 7 cut(s) 140, 645, 731, 897, 1137, 1261, 1786
AvaII GGWCC 2 cut(s) 1049, 1321
AxyI CCTNAGG 1 cut(s) 1707
BalI TGGCCA 2 cut(s) 834, 1505
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
Bbv12I GWGCWC 3 cut(s) 61, 550, 1168
BbvI GCAGC 2 cut(s) 1004, 1072
BccI CCATC 2 cut(s) 1150, 1189
BceAI ACGGC 3 cut(s) 28, 29, 124
BcgI CGANNNNNNTGC 2 cut(s) 1726, 1760
BciT130I CCWGG 2 cut(s) 336, 668
BciVI GTATCC 3 cut(s) 776, 1018, 1727
BclI TGATCA 1 cut(s) 1822
BcoDI GTCTC 4 cut(s) 51, 276, 315, 734
BfaI CTAG 6 cut(s) 407, 800, 942, 954, 1260, 1268
BfmI CTRYAG 2 cut(s) 1401, 1572
BfoI RGCGCY 2 cut(s) 8, 806
BfrI CTTAAG 1 cut(s) 1616
BfuI GTATCC 3 cut(s) 776, 1018, 1727
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 2 cut(s) 993, 1086
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 2 cut(s) 994, 1087
Bme1390I CCNGG 2 cut(s) 336, 668
Bme18I GGWCC 2 cut(s) 1049, 1321
BmgT120I GGNCC 3 cut(s) 1049, 1321, 1516
BmiI GGNNCC 2 cut(s) 423, 1323
BmrFI CCNGG 2 cut(s) 336, 668
BmrI ACTGGG 2 cut(s) 20, 1453
BmsI GCATC 7 cut(s) 249, 469, 850, 872, 1052, 1166, 1438
BmuI ACTGGG 2 cut(s) 20, 1453
BoxI GACNNNNGTC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 120
BpuEI CTTGAG 2 cut(s) 216, 270
BsaJI CCNNGG 3 cut(s) 203, 667, 1633
BsaWI WCCGGW 1 cut(s) 1426
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 1708, 1760
Bse118I RCCGGY 2 cut(s) 262, 1783
Bse1I ACTGG 9 cut(s) 26, 175, 926, 969, 1323, 1448, 1673, 1713, 1754
Bse21I CCTNAGG 1 cut(s) 1707
Bse3DI GCAATG 3 cut(s) 294, 1031, 1061
BseBI CCWGG 2 cut(s) 336, 668
BseDI CCNNGG 3 cut(s) 203, 667, 1633
BseGI GGATG 7 cut(s) 264, 484, 865, 1081, 1121, 1453, 1671
BseLI CCNNNNNNNGG 3 cut(s) 209, 1708, 1760
BseMI GCAATG 3 cut(s) 294, 1031, 1061
BseMII CTCAG 7 cut(s) 44, 111, 516, 1500, 1584, 1721, 1878
BseNI ACTGG 9 cut(s) 26, 175, 926, 969, 1323, 1448, 1673, 1713, 1754
BseRI GAGGAG 3 cut(s) 61, 587, 612
BseXI GCAGC 2 cut(s) 1004, 1072
BshFI GGCC 6 cut(s) 148, 834, 1505, 1518, 1712, 1783
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 3 cut(s) 61, 550, 1168
BsiSI CCGG 3 cut(s) 263, 1427, 1784
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 3 cut(s) 209, 1708, 1760
BsmAI GTCTC 4 cut(s) 51, 276, 315, 734
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 2 cut(s) 892, 1546
BsnI GGCC 6 cut(s) 148, 834, 1505, 1518, 1712, 1783
Bsp1286I GDGCHC 3 cut(s) 61, 550, 1168
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 2 cut(s) 1243, 1289
BspANI GGCC 6 cut(s) 148, 834, 1505, 1518, 1712, 1783
BspCNI CTCAG 7 cut(s) 45, 112, 517, 1501, 1585, 1720, 1877
BspHI TCATGA 2 cut(s) 178, 934
BspLI GGNNCC 2 cut(s) 423, 1323
BspMAI CTGCAG 1 cut(s) 1576
BspPI GGATC 5 cut(s) 230, 529, 989, 1353, 1686
BspT107I GGYRCC 1 cut(s) 421
BspTI CTTAAG 1 cut(s) 1616
BsrDI GCAATG 3 cut(s) 294, 1031, 1061
BsrFI RCCGGY 2 cut(s) 262, 1783
BsrI ACTGG 9 cut(s) 26, 175, 926, 969, 1323, 1448, 1673, 1713, 1754
BssAI RCCGGY 2 cut(s) 262, 1783
BssECI CCNNGG 3 cut(s) 203, 667, 1633
BssT1I CCWWGG 2 cut(s) 203, 1633
Bst2UI CCWGG 2 cut(s) 336, 668
Bst4CI ACNGT 7 cut(s) 250, 431, 472, 812, 853, 880, 1149
Bst6I CTCTTC 3 cut(s) 564, 771, 1332
BstAFI CTTAAG 1 cut(s) 1616
BstAPI GCANNNNNTGC 2 cut(s) 1163, 1571
BstC8I GCNNGC 4 cut(s) 150, 264, 645, 1785
BstDEI CTNAG 9 cut(s) 53, 120, 525, 792, 1337, 1509, 1593, 1707, 1864
BstF5I GGATG 7 cut(s) 264, 484, 865, 1081, 1121, 1453, 1671
BstH2I RGCGCY 2 cut(s) 8, 806
BstHHI GCGC 3 cut(s) 7, 43, 805
BstMAI GTCTC 4 cut(s) 51, 276, 315, 734
BstMWI GCNNNNNNNGC 5 cut(s) 13, 145, 1163, 1571, 1598
BstNI CCWGG 2 cut(s) 336, 668
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 2 cut(s) 334, 666
BstSFI CTRYAG 2 cut(s) 1401, 1572
BstV1I GCAGC 2 cut(s) 1004, 1072
BstX2I RGATCY 3 cut(s) 235, 521, 1345
BstXI CCANNNNNNTGG 1 cut(s) 418
BstYI RGATCY 3 cut(s) 235, 521, 1345
Bsu36I CCTNAGG 1 cut(s) 1707
BsuI GTATCC 3 cut(s) 776, 1018, 1727
BsuRI GGCC 6 cut(s) 148, 834, 1505, 1518, 1712, 1783
BtsCI GGATG 7 cut(s) 264, 484, 865, 1081, 1121, 1453, 1671
BtsI GCAGTG 1 cut(s) 904
BtsIMutI CAGTG 9 cut(s) 33, 109, 168, 904, 1412, 1506, 1680, 1761, 1891
Cac8I GCNNGC 4 cut(s) 150, 264, 645, 1785
CciI TCATGA 2 cut(s) 178, 934
CfoI GCGC 3 cut(s) 7, 43, 805
Cfr10I RCCGGY 2 cut(s) 262, 1783
Cfr13I GGNCC 3 cut(s) 1049, 1321, 1516
Csp6I GTAC 5 cut(s) 96, 578, 813, 1059, 1424
CspCI CAANNNNNGTGG 4 cut(s) 576, 611, 1482, 1517
CviAII CATG 6 cut(s) 163, 179, 935, 1016, 1055, 1169
CviQI GTAC 5 cut(s) 96, 578, 813, 1059, 1424
DdeI CTNAG 9 cut(s) 53, 120, 525, 792, 1337, 1509, 1593, 1707, 1864
DraIII CACNNNGTG 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 832, 1503
Eam1104I CTCTTC 3 cut(s) 564, 771, 1332
EarI CTCTTC 3 cut(s) 564, 771, 1332
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 203, 1633
Eco24I GRGCYC 1 cut(s) 61
Eco32I GATATC 2 cut(s) 490, 1891
Eco47I GGWCC 2 cut(s) 1049, 1321
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 3 cut(s) 218, 1058, 1867
Eco81I CCTNAGG 1 cut(s) 1707
EcoICRI GAGCTC 1 cut(s) 59
EcoO109I RGGNCCY 2 cut(s) 1049, 1516
EcoRI GAATTC 1 cut(s) 1628
EcoRII CCWGG 2 cut(s) 334, 666
EcoRV GATATC 2 cut(s) 490, 1891
EcoT14I CCWWGG 2 cut(s) 203, 1633
EcoT22I ATGCAT 1 cut(s) 865
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 203, 1633
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 6 cut(s) 166, 182, 938, 1019, 1058, 1172
FalI AAGNNNNNCTT 2 cut(s) 1455, 1487
FaqI GGGAC 1 cut(s) 465
FatI CATG 6 cut(s) 162, 178, 934, 1015, 1054, 1168
FbaI TGATCA 1 cut(s) 1822
Fnu4HI GCNGC 2 cut(s) 993, 1086
FokI GGATG 7 cut(s) 271, 491, 872, 1088, 1108, 1460, 1678
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 993, 1086
FspBI CTAG 6 cut(s) 407, 800, 942, 954, 1260, 1268
GlaI GCGC 3 cut(s) 6, 42, 804
GluI GCNGC 2 cut(s) 993, 1086
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 2 cut(s) 8, 806
HaeIII GGCC 6 cut(s) 148, 834, 1505, 1518, 1712, 1783
HapII CCGG 3 cut(s) 263, 1427, 1784
HhaI GCGC 3 cut(s) 7, 43, 805
Hin1II CATG 6 cut(s) 166, 182, 938, 1019, 1058, 1172
Hin6I GCGC 3 cut(s) 5, 41, 803
HinP1I GCGC 3 cut(s) 5, 41, 803
HindIII AAGCTT 1 cut(s) 1613
HinfI GANTC 6 cut(s) 283, 1187, 1211, 1271, 1478, 1525
HpaII CCGG 3 cut(s) 263, 1427, 1784
HphI GGTGA 7 cut(s) 140, 645, 731, 897, 1137, 1261, 1786
Hpy166II GTNNAC 3 cut(s) 78, 687, 905
Hpy188III TCNNGA 9 cut(s) 52, 179, 233, 256, 392, 773, 872, 935, 1098
Hpy8I GTNNAC 3 cut(s) 78, 687, 905
Hpy99I CGWCG 1 cut(s) 1772
HpyCH4III ACNGT 7 cut(s) 250, 431, 472, 812, 853, 880, 1149
HpyCH4IV ACGT 1 cut(s) 1873
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 145, 1163, 1571, 1598
HpyF3I CTNAG 9 cut(s) 53, 120, 525, 792, 1337, 1509, 1593, 1707, 1864
HpySE526I ACGT 1 cut(s) 1873
Hsp92II CATG 6 cut(s) 166, 182, 938, 1019, 1058, 1172
HspAI GCGC 3 cut(s) 5, 41, 803
KroI GCCGGC 2 cut(s) 262, 1783
KroNI GCCGGC 2 cut(s) 264, 1785
Ksp22I TGATCA 1 cut(s) 1822
Lsp1109I GCAGC 2 cut(s) 1004, 1072
LweI GCATC 7 cut(s) 249, 469, 850, 872, 1052, 1166, 1438
MaeI CTAG 6 cut(s) 407, 800, 942, 954, 1260, 1268
MaeII ACGT 1 cut(s) 1873
MaeIII GTNAC 4 cut(s) 290, 847, 949, 1792
MboII GAAGA 8 cut(s) 322, 407, 581, 788, 929, 1232, 1319, 1549
MfeI CAATTG 3 cut(s) 840, 1494, 1530
MflI RGATCY 3 cut(s) 235, 521, 1345
MhlI GDGCHC 3 cut(s) 61, 550, 1168
MlsI TGGCCA 2 cut(s) 834, 1505
MluNI TGGCCA 2 cut(s) 834, 1505
MlyI GAGTC 3 cut(s) 277, 1196, 1519
MmeI TCCRAC 1 cut(s) 1828
Mox20I TGGCCA 2 cut(s) 834, 1505
Mph1103I ATGCAT 1 cut(s) 865
MroNI GCCGGC 2 cut(s) 262, 1783
MroXI GAANNNNTTC 3 cut(s) 402, 1224, 1467
MscI TGGCCA 2 cut(s) 834, 1505
MseI TTAA 5 cut(s) 143, 681, 1368, 1380, 1617
MslI CAYNNNNRTG 5 cut(s) 257, 555, 638, 1686, 1799
Msp20I TGGCCA 2 cut(s) 834, 1505
MspCI CTTAAG 1 cut(s) 1616
MspI CCGG 3 cut(s) 263, 1427, 1784
MspR9I CCNGG 2 cut(s) 336, 668
MunI CAATTG 3 cut(s) 840, 1494, 1530
Mva1269I GAATGC 2 cut(s) 892, 1546
MvaI CCWGG 2 cut(s) 336, 668
MwoI GCNNNNNNNGC 5 cut(s) 13, 145, 1163, 1571, 1598
NaeI GCCGGC 2 cut(s) 264, 1785
NgoMIV GCCGGC 2 cut(s) 262, 1783
NlaIII CATG 6 cut(s) 166, 182, 938, 1019, 1058, 1172
NlaIV GGNNCC 2 cut(s) 423, 1323
NmuCI GTSAC 1 cut(s) 1792
NsiI ATGCAT 1 cut(s) 865
PagI TCATGA 2 cut(s) 178, 934
PctI GAATGC 2 cut(s) 892, 1546
PdiI GCCGGC 2 cut(s) 264, 1785
PdmI GAANNNNTTC 3 cut(s) 402, 1224, 1467
PfeI GAWTC 3 cut(s) 1211, 1271, 1478
PkrI GCNGC 2 cut(s) 994, 1087
PleI GAGTC 3 cut(s) 277, 1195, 1519
PpsI GAGTC 3 cut(s) 277, 1195, 1519
PpuMI RGGWCCY 1 cut(s) 1049
PshAI GACNNNNGTC 1 cut(s) 212
PsiI TTATAA 1 cut(s) 1697
Psp124BI GAGCTC 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 1049
Psp6I CCWGG 2 cut(s) 334, 666
PspGI CCWGG 2 cut(s) 334, 666
PspN4I GGNNCC 2 cut(s) 423, 1323
PspPI GGNCC 3 cut(s) 1049, 1321, 1516
PspPPI RGGWCCY 1 cut(s) 1049
PstI CTGCAG 1 cut(s) 1576
PsuI RGATCY 3 cut(s) 235, 521, 1345
RsaI GTAC 5 cut(s) 97, 579, 814, 1060, 1425
RsaNI GTAC 5 cut(s) 96, 578, 813, 1059, 1424
RseI CAYNNNNRTG 5 cut(s) 257, 555, 638, 1686, 1799
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 5 cut(s) 143, 681, 1368, 1380, 1617
SatI GCNGC 2 cut(s) 993, 1086
Sau96I GGNCC 3 cut(s) 1049, 1321, 1516
SchI GAGTC 3 cut(s) 277, 1196, 1519
ScrFI CCNGG 2 cut(s) 336, 668
SduI GDGCHC 3 cut(s) 61, 550, 1168
SfaNI GCATC 7 cut(s) 249, 469, 850, 872, 1052, 1166, 1438
SfcI CTRYAG 2 cut(s) 1401, 1572
SinI GGWCC 2 cut(s) 1049, 1321
SmiMI CAYNNNNRTG 5 cut(s) 257, 555, 638, 1686, 1799
SmlI CTYRAG 3 cut(s) 231, 285, 1616
SmoI CTYRAG 3 cut(s) 231, 285, 1616
SsiI CCGC 2 cut(s) 1243, 1289
SspI AATATT 1 cut(s) 676
SspMI CTAG 6 cut(s) 407, 800, 942, 954, 1260, 1268
SstI GAGCTC 1 cut(s) 61
StyD4I CCNGG 2 cut(s) 334, 666
StyI CCWWGG 2 cut(s) 203, 1633
TaaI ACNGT 7 cut(s) 250, 431, 472, 812, 853, 880, 1149
TaiI ACGT 1 cut(s) 1876
TaqI TCGA 2 cut(s) 61, 1828
TfiI GAWTC 3 cut(s) 1211, 1271, 1478
Tru1I TTAA 5 cut(s) 143, 681, 1368, 1380, 1617
Tru9I TTAA 5 cut(s) 143, 681, 1368, 1380, 1617
TscAI CASTG 9 cut(s) 33, 109, 175, 904, 1419, 1513, 1680, 1761, 1891
TseFI GTSAC 1 cut(s) 1792
TseI GCWGC 2 cut(s) 992, 1085
Tsp45I GTSAC 1 cut(s) 1792
TspDTI ATGAA 4 cut(s) 167, 1110, 1641, 1817
TspGWI ACGGA 3 cut(s) 920, 1785, 1854
TspRI CASTG 9 cut(s) 33, 109, 175, 904, 1419, 1513, 1680, 1761, 1891
Vha464I CTTAAG 1 cut(s) 1616
VpaK11BI GGWCC 2 cut(s) 1049, 1321
XapI RAATTY 4 cut(s) 112, 380, 761, 1628
XmnI GAANNNNTTC 3 cut(s) 402, 1224, 1467
XspI CTAG 6 cut(s) 407, 800, 942, 954, 1260, 1268
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.