MD01G1105500.v1.1

Arsenical pump-driving

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010871
Physical Location & Seq
Reverse (-)
21892601 .. 21897525
4925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1105500.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGCGAGTAGCACATGTATTCCCTCCACTTTCACACCTATTCTACACAACTTAACCGCCAGAAACTCCATAACAATGGTGGGTTTGCTCTCTCATGCTCCCAAAATCCTCAGGCCTCTCTCTCTCCCCAAAAGCCTCGGTTTCAGCTCACTTTCCACCGCCACAAAACCCCCCAGAAAATTCTTCCATTTGCAAGTGAGATCAGTGGCCACTCCAGCAGAAGCTGTTGCCGGGTTTGATGACATGGTTGCTGGGACTCAGAGAAAGTATTACATGTTAGGTGGGAAGGGCGGTGTAGGGAAGACAAGTTGTGCTGCATCACTTGCCGTAAAATTCGCTAACAATGGGCATCCCACTCTTGTGGTTTCCACTGATCCAGCACATTCCTTGAGTGATTCCTTTGCTCAGGATTTGGCTGGAGGAACACTTGTACCAGTTGATGGGCCTGAAGCTCCGCTTTTTGCTCTTGAGATAAACCCTGAGAAGGCAAGGGAAGAATTCCGTAATGTAAGTCAGAAAAATGGTGGAACAGGAGTCAAAGATTTCATGGATGGTATGGGCCTTGGGATGCTTTCGGAACAGTTAGGAGAATTAAAACTGGGAGAATTACTAGACACGCCTCCTCCTGGTTTGGATGAAGCTATTGCAATTTCCAAGGTGATACAATTTCTTGAATCCCCGGAATATAGCATGTTTACTCGTATAGTTTTTGATACTGCACCCACGGGTCACACATTGCGGCTTTTGTCCTTGCCAGACTTTTTGGATGCATCAATTGGTAAAATATTGAAGCTTAAACAAAAGATATCATCAGCCACCTCAGCGATCAAATCTGTTTTTGGGCAAGAGCAACCCCAATTGGGCGCGGCTGACAAATTAGAGAAACTAAGGGAGAGGATGATTAAAGTCCGTGAACTTTTTCGTGACACTGAGTCAACAGAGTTTGTCATAGTAACAATACCGACGGTGATGGCCGTTAGTGAGTCATCTAGATTGCATGCCTCTCTGAAGAAGGAAAGTGTTCCGGTTACTCGACTTATAGTTAATCAGATTCTTCCCCCTTCTGCCTCGGACTGCAAATTTTGTTCAATTAAAAGAAAGGATCAAATGCGTGCTCTTGATATGATCCGGAGCGATACAGAGCTATCCGGCTTGACAGTGATCCAGGCTCCACTAGTTGATGTGGAGATCAGAGGCGTTCCTGCCCTTAGATTTCTGGGAGACATAATTTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

413

Amino Acids

44.53

Weight (kDa)

8.75

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArsA_ATPase PF02374 89 - 410 6.8e-73 Anion-transporting ATPase
CbiA PF01656 93 - 299 4.5e-08 CobQ/CobB/MinD/ParA nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10350
fragaria_vesca FvH4_7g18990 FvH4_7g18990
malus_domestica MD01G1105500.v1.1 MD07G1171700.v1.1
prunus_persica Prupe.2G211000_v2.0.a1 Prupe.2G211000_v2.0.a1 Prupe.2G211000_v2.0.a1
pyrus_communis pycom01g13230 pycom07g16630
rosa_chinensis RchiOBHm_Chr1g0362921
rosa_laevigata RLG00000027612
rosa_multiflora Rmu_sc0001502.1_g000006
rosa_roxburghii Rroxscaffold_4G00293650
rosa_rugosa Rorug01G0302100
rosa_samantha Rh1AG311600 Rh1BG274700 Rh1CG291900 Rh1DG305400
rosa_wichuraiana Rw1G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 440
AccII CGCG 1 cut(s) 866
AccIII TCCGGA 1 cut(s) 1128
AciI CCGC 6 cut(s) 57, 159, 291, 455, 739, 866
AclWI GGATC 4 cut(s) 368, 1110, 1120, 1156
AcoI YGGCCR 2 cut(s) 207, 972
AcsI RAATTY 4 cut(s) 179, 332, 497, 1079
AcuI CTGAAG 2 cut(s) 468, 1028
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 4 cut(s) 440, 484, 626, 860
AflIII ACRYGT 2 cut(s) 14, 273
AgsI TTSAA 3 cut(s) 674, 790, 1089
AhdI GACNNNNNGTC 1 cut(s) 931
AhlI ACTAGT 1 cut(s) 1174
AjnI CCWGG 2 cut(s) 625, 1164
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 6 cut(s) 147, 224, 452, 641, 793, 1144
AluI AGCT 6 cut(s) 147, 224, 452, 641, 793, 1144
Alw21I GWGCWC 1 cut(s) 1117
Alw26I GTCTC 1 cut(s) 1215
AlwI GGATC 4 cut(s) 368, 1110, 1120, 1156
AlwNI CAGNNNCTG 1 cut(s) 224
Aor13HI TCCGGA 1 cut(s) 1128
AoxI GGCC 5 cut(s) 113, 207, 443, 559, 972
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 4 cut(s) 179, 332, 497, 1079
Asp700I GAANNNNTTC 2 cut(s) 918, 1020
AspLEI GCGC 1 cut(s) 866
AspS9I GGNCC 2 cut(s) 443, 559
AsuC2I CCSGG 2 cut(s) 232, 680
AsuHPI GGTGA 2 cut(s) 670, 979
AxyI CCTNAGG 1 cut(s) 110
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BalI TGGCCA 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 1117
BbvCI CCTCAGC 1 cut(s) 820
BbvI GCAGC 1 cut(s) 301
BccI CCATC 3 cut(s) 434, 545, 964
BceAI ACGGC 2 cut(s) 311, 959
BciT130I CCWGG 2 cut(s) 627, 1166
BcnI CCSGG 2 cut(s) 232, 680
BcoDI GTCTC 1 cut(s) 1215
BcuI ACTAGT 1 cut(s) 1174
BfaI CTAG 3 cut(s) 611, 990, 1175
BisI GCNGC 3 cut(s) 315, 740, 867
BlsI GCNGC 3 cut(s) 316, 741, 868
Bme1390I CCNGG 4 cut(s) 232, 627, 680, 1166
BmeRI GACNNNNNGTC 1 cut(s) 931
BmgT120I GGNCC 2 cut(s) 443, 559
BmiI GGNNCC 1 cut(s) 1170
BmrFI CCNGG 4 cut(s) 232, 627, 680, 1166
BmrI ACTGGG 1 cut(s) 608
BmsI GCATC 5 cut(s) 326, 358, 558, 757, 779
BmuI ACTGGG 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 2 cut(s) 198, 438
Bpu10I CCTNAGC 2 cut(s) 405, 820
BpuEI CTTGAG 2 cut(s) 409, 488
BpuMI CCSGG 2 cut(s) 232, 680
BsaJI CCNNGG 6 cut(s) 136, 562, 654, 678, 723, 1068
BsaWI WCCGGW 2 cut(s) 1024, 1128
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 4 cut(s) 440, 484, 626, 860
Bse1I ACTGG 2 cut(s) 434, 603
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 734
BseAI TCCGGA 1 cut(s) 1128
BseBI CCWGG 2 cut(s) 627, 1166
BseDI CCNNGG 6 cut(s) 136, 562, 654, 678, 723, 1068
BseGI GGATG 6 cut(s) 349, 556, 573, 640, 772, 903
BseLI CCNNNNNNNGG 4 cut(s) 440, 484, 626, 860
BseMI GCAATG 1 cut(s) 734
BseMII CTCAG 6 cut(s) 124, 272, 419, 471, 834, 921
BseNI ACTGG 2 cut(s) 434, 603
BseRI GAGGAG 1 cut(s) 612
BseXI GCAGC 1 cut(s) 301
BseYI CCCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 866
BshFI GGCC 5 cut(s) 115, 209, 445, 561, 974
BsiHKAI GWGCWC 1 cut(s) 1117
BsiSI CCGG 5 cut(s) 231, 680, 1025, 1129, 1149
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 4 cut(s) 440, 484, 626, 860
BsmAI GTCTC 1 cut(s) 1215
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 5 cut(s) 115, 209, 445, 561, 974
Bsp1286I GDGCHC 1 cut(s) 1117
Bsp13I TCCGGA 1 cut(s) 1128
Bsp143I GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
BspACI CCGC 6 cut(s) 57, 159, 291, 455, 739, 866
BspANI GGCC 5 cut(s) 115, 209, 445, 561, 974
BspCNI CTCAG 6 cut(s) 123, 271, 418, 472, 833, 922
BspEI TCCGGA 1 cut(s) 1128
BspFNI CGCG 1 cut(s) 866
BspLI GGNNCC 1 cut(s) 1170
BspPI GGATC 4 cut(s) 368, 1110, 1120, 1156
BsrDI GCAATG 1 cut(s) 734
BsrI ACTGG 2 cut(s) 434, 603
BssECI CCNNGG 6 cut(s) 136, 562, 654, 678, 723, 1068
BssMI GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
BssT1I CCWWGG 2 cut(s) 562, 654
Bst2UI CCWGG 2 cut(s) 627, 1166
Bst4CI ACNGT 3 cut(s) 582, 967, 1159
BstAPI GCANNNNNTGC 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 999, 1113
BstDEI CTNAG 8 cut(s) 110, 258, 405, 480, 820, 887, 930, 1208
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 6 cut(s) 349, 556, 573, 640, 772, 903
BstFNI CGCG 1 cut(s) 866
BstHHI GCGC 1 cut(s) 866
BstKTI GATC 7 cut(s) 203, 376, 828, 1105, 1128, 1164, 1191
BstMAI GTCTC 1 cut(s) 1215
BstMBI GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
BstMWI GCNNNNNNNGC 3 cut(s) 215, 323, 821
BstNI CCWGG 2 cut(s) 627, 1166
BstNSI RCATGY 4 cut(s) 18, 277, 694, 1001
BstSCI CCNGG 4 cut(s) 230, 625, 678, 1164
BstUI CGCG 1 cut(s) 866
BstV1I GCAGC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 308
BstXI CCANNNNNNTGG 2 cut(s) 76, 361
Bsu36I CCTNAGG 1 cut(s) 110
BsuRI GGCC 5 cut(s) 115, 209, 445, 561, 974
BtgI CCRYGG 1 cut(s) 723
BtsCI GGATG 6 cut(s) 349, 556, 573, 640, 772, 903
BtsIMutI CAGTG 4 cut(s) 210, 369, 927, 1164
Cac8I GCNNGC 2 cut(s) 999, 1113
CaiI CAGNNNCTG 1 cut(s) 224
CfoI GCGC 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 443, 559
Csp6I GTAC 1 cut(s) 431
CviAII CATG 7 cut(s) 15, 95, 244, 274, 547, 691, 998
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 8 cut(s) 110, 258, 405, 480, 820, 887, 930, 1208
DpnI GATC 7 cut(s) 202, 375, 827, 1104, 1127, 1163, 1190
DpnII GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
DriI GACNNNNNGTC 1 cut(s) 931
EaeI YGGCCR 2 cut(s) 207, 972
Eam1105I GACNNNNNGTC 1 cut(s) 931
Eco130I CCWWGG 2 cut(s) 562, 654
Eco147I AGGCCT 1 cut(s) 115
Eco32I GATATC 1 cut(s) 807
Eco57I CTGAAG 2 cut(s) 468, 1028
Eco81I CCTNAGG 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 2 cut(s) 625, 1164
EcoRV GATATC 1 cut(s) 807
EcoT14I CCWWGG 2 cut(s) 562, 654
EcoT22I ATGCAT 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 562, 654
FaeI CATG 7 cut(s) 18, 98, 247, 277, 550, 694, 1001
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 1 cut(s) 268
FatI CATG 7 cut(s) 14, 94, 243, 273, 546, 690, 997
Fnu4HI GCNGC 3 cut(s) 315, 740, 867
FokI GGATG 6 cut(s) 336, 563, 580, 647, 779, 910
Fsp4HI GCNGC 3 cut(s) 315, 740, 867
FspBI CTAG 3 cut(s) 611, 990, 1175
GlaI GCGC 1 cut(s) 865
GluI GCNGC 3 cut(s) 315, 740, 867
GsaI CCCAGC 1 cut(s) 255
GsuI CTGGAG 2 cut(s) 198, 438
HaeIII GGCC 5 cut(s) 115, 209, 445, 561, 974
HapII CCGG 5 cut(s) 231, 680, 1025, 1129, 1149
HhaI GCGC 1 cut(s) 866
Hin1II CATG 7 cut(s) 18, 98, 247, 277, 550, 694, 1001
Hin6I GCGC 1 cut(s) 864
HinP1I GCGC 1 cut(s) 864
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 7 cut(s) 256, 395, 534, 674, 932, 983, 1051
HpaII CCGG 5 cut(s) 231, 680, 1025, 1129, 1149
HphI GGTGA 2 cut(s) 670, 979
Hpy166II GTNNAC 3 cut(s) 696, 914, 936
Hpy188I TCNGA 7 cut(s) 261, 516, 577, 1008, 1050, 1072, 1193
Hpy188III TCNNGA 7 cut(s) 407, 467, 671, 923, 990, 1118, 1129
Hpy8I GTNNAC 3 cut(s) 696, 914, 936
Hpy99I CGWCG 1 cut(s) 967
HpyAV CCTTC 4 cut(s) 280, 478, 1006, 1071
HpyCH4III ACNGT 3 cut(s) 582, 967, 1159
HpyCH4V TGCA 7 cut(s) 193, 317, 647, 719, 770, 997, 1077
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 323, 821
HpyF3I CTNAG 8 cut(s) 110, 258, 405, 480, 820, 887, 930, 1208
Hsp92II CATG 7 cut(s) 18, 98, 247, 277, 550, 694, 1001
HspAI GCGC 1 cut(s) 864
Kpn2I TCCGGA 1 cut(s) 1128
Kzo9I GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
LmnI GCTCC 4 cut(s) 103, 457, 1131, 1174
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 5 cut(s) 326, 358, 558, 757, 779
MaeI CTAG 3 cut(s) 611, 990, 1175
MaeIII GTNAC 4 cut(s) 728, 923, 952, 1027
MalI GATC 7 cut(s) 202, 375, 827, 1104, 1127, 1163, 1190
MboI GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
MboII GAAGA 5 cut(s) 175, 313, 506, 1021, 1046
MfeI CAATTG 2 cut(s) 774, 857
MhlI GDGCHC 1 cut(s) 1117
MlsI TGGCCA 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 4 cut(s) 250, 543, 941, 992
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 772
MroI TCCGGA 1 cut(s) 1128
MroXI GAANNNNTTC 2 cut(s) 918, 1020
MscI TGGCCA 1 cut(s) 209
MseI TTAA 6 cut(s) 53, 593, 795, 903, 1044, 1092
MslI CAYNNNNRTG 2 cut(s) 74, 359
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 5 cut(s) 231, 680, 1025, 1129, 1149
MspR9I CCNGG 4 cut(s) 232, 627, 680, 1166
MunI CAATTG 2 cut(s) 774, 857
MvaI CCWGG 2 cut(s) 627, 1166
MvnI CGCG 1 cut(s) 866
MwoI GCNNNNNNNGC 3 cut(s) 215, 323, 821
NciI CCSGG 2 cut(s) 232, 680
NdeII GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
NlaIII CATG 7 cut(s) 18, 98, 247, 277, 550, 694, 1001
NlaIV GGNNCC 1 cut(s) 1170
NmuCI GTSAC 2 cut(s) 728, 923
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 4 cut(s) 18, 277, 694, 1001
OliI CACNNNNGTG 1 cut(s) 359
PaeI GCATGC 1 cut(s) 1001
PceI AGGCCT 1 cut(s) 115
PciI ACATGT 2 cut(s) 14, 273
PdmI GAANNNNTTC 2 cut(s) 918, 1020
PfeI GAWTC 3 cut(s) 395, 674, 1051
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 3 cut(s) 316, 741, 868
PleI GAGTC 4 cut(s) 250, 542, 940, 991
PpsI GAGTC 4 cut(s) 250, 542, 940, 991
PscI ACATGT 2 cut(s) 14, 273
Psp6I CCWGG 2 cut(s) 625, 1164
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 2 cut(s) 625, 1164
PspN4I GGNNCC 1 cut(s) 1170
PspPI GGNCC 2 cut(s) 443, 559
PstNI CAGNNNCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
RseI CAYNNNNRTG 2 cut(s) 74, 359
SaqAI TTAA 6 cut(s) 53, 593, 795, 903, 1044, 1092
SatI GCNGC 3 cut(s) 315, 740, 867
Sau3AI GATC 7 cut(s) 200, 373, 825, 1102, 1125, 1161, 1188
Sau96I GGNCC 2 cut(s) 443, 559
SchI GAGTC 4 cut(s) 250, 543, 941, 992
ScrFI CCNGG 4 cut(s) 232, 627, 680, 1166
SduI GDGCHC 1 cut(s) 1117
SfaNI GCATC 5 cut(s) 326, 358, 558, 757, 779
SmiMI CAYNNNNRTG 2 cut(s) 74, 359
SmlI CTYRAG 2 cut(s) 388, 467
SmoI CTYRAG 2 cut(s) 388, 467
SpeI ACTAGT 1 cut(s) 1174
SphI GCATGC 1 cut(s) 1001
SseBI AGGCCT 1 cut(s) 115
SsiI CCGC 6 cut(s) 57, 159, 291, 455, 739, 866
SspI AATATT 1 cut(s) 786
SspMI CTAG 3 cut(s) 611, 990, 1175
StuI AGGCCT 1 cut(s) 115
StyD4I CCNGG 4 cut(s) 230, 625, 678, 1164
StyI CCWWGG 2 cut(s) 562, 654
TaaI ACNGT 3 cut(s) 582, 967, 1159
TaqI TCGA 1 cut(s) 1033
TauI GCSGC 2 cut(s) 742, 869
TfiI GAWTC 3 cut(s) 395, 674, 1051
Tru1I TTAA 6 cut(s) 53, 593, 795, 903, 1044, 1092
Tru9I TTAA 6 cut(s) 53, 593, 795, 903, 1044, 1092
TscAI CASTG 4 cut(s) 210, 376, 934, 1164
TseFI GTSAC 2 cut(s) 728, 923
TseI GCWGC 1 cut(s) 314
Tsp45I GTSAC 2 cut(s) 728, 923
TspDTI ATGAA 2 cut(s) 535, 651
TspGWI ACGGA 2 cut(s) 491, 899
TspRI CASTG 4 cut(s) 210, 376, 934, 1164
Van91I CCANNNNNTGG 1 cut(s) 440
XapI RAATTY 4 cut(s) 179, 332, 497, 1079
XbaI TCTAGA 1 cut(s) 989
XceI RCATGY 4 cut(s) 18, 277, 694, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmnI GAANNNNTTC 2 cut(s) 918, 1020
XspI CTAG 3 cut(s) 611, 990, 1175
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.