Rw1G027610

Arsenical pump-driving

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010871
Physical Location & Seq
Reverse (-)
55134155 .. 55138093
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027610.1

Sequence Viewer

Length: 1239 bp
ATGGCGACTAGTACGTGTATTCCCTCCACCTTCACACCCATTCTTCAAAACTTGACTTCCAGAAACTCCATTGCTATGGTGGGTTTGCTCTCTTATGCTCCCAAAACCTTGAAGCCTCTCTCTTATGCCCAGAGCTTCGGTTTCAGTTCACTTTCCACCGCCAGAAAACCGCCCAGAAAATTGCTTCAATTCCAAGTGAGAGCAGTGGCCACTCCTGCGGAAGCTGTTGCTGGGTTTGAAGAGATGGTTGCTGGGACTCAGCGAAAGTATTACATGCTAGGTGGTAAAGGTGGAGTTGGAAAGACGAGCTGTGCAGCTTCACTTGCTGTAAAGTTTGCTAACAATGGGCATCCCACTCTTGTGGTTTCCACTGATCCAGCACATTCCTTGAGTGATTCCTTTGCTCAGGATTTGACTGGAGGCACACTTGTACCAGTTGATGGGCCTGATGCTCCACTTTTTGCTCTGGAGATAAACCCTGAGAAGGCAAGGGAAGAATTCAAAACTGCAAGTCAGAAAAATGGTGGAACAGGGGTCAAAGATTTTATGGATGGAATGGGCCTTGGGATGCTTGCTGAGCAGTTGGGAGAGTTAAAACTGGGAGAACTGCTAGACACACCTCCTCCTGGTTTGGATGAAGCAATTGCGATTTCTAAGGTGATACAATTTCTTGAATCACCAGAATATAGCATGTTTACTCGTATCGTCTTTGATACTGCACCCACGGGCCATACATTGCGACTTTTATCCTTGCCAGACTTCTTGGATGCATCAATTGGCAAGATATTGAAGCTTAGACAAAAGATATCTTCTGCCACCTCAGCCATCAAATCCGTTTTTGGGTCAGGGGAAAGCAATTCTGGAGCATCAGACAAATTAGAGAAACTACGGGAGAGGATGATTAAAGTGCGGGAGCTTTTTCGTGATACCGATTCCACAGAATTTGTCATAGTAACAATACCCACGGTCATGGCAGTCAGTGAGTCATCTAGGTTGCATGCCTCTTTGAAGAAGGAAACTGTTCCTGTGAAGCGACTTATTGTTAATCAGATTCTTCCCCCATCTGCCTCAGACTGCAAATTTTGTTCAATAAAAAGAAAGGATCAGATGCGTGCTCTTGATATGATCCGGAGTGATATAGAGCTCTCTGACTTGACATTGATCCAGGCTCCACTAGTTGACATGGAGATCAGAGGAGTGCCTGCCCTTCGATTTCTGGGAGACATGATTTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

412

Amino Acids

44.56

Weight (kDa)

8.72

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArsA_ATPase PF02374 89 - 410 6.5e-74 Anion-transporting ATPase
CbiA PF01656 93 - 350 3.5e-08 CobQ/CobB/MinD/ParA nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10350
fragaria_vesca FvH4_7g18990 FvH4_7g18990
malus_domestica MD01G1105500.v1.1 MD07G1171700.v1.1
prunus_persica Prupe.2G211000_v2.0.a1 Prupe.2G211000_v2.0.a1 Prupe.2G211000_v2.0.a1
pyrus_communis pycom01g13230 pycom07g16630
rosa_chinensis RchiOBHm_Chr1g0362921
rosa_laevigata RLG00000027612
rosa_multiflora Rmu_sc0001502.1_g000006
rosa_roxburghii Rroxscaffold_4G00293650
rosa_rugosa Rorug01G0302100
rosa_samantha Rh1AG311600 Rh1BG274700 Rh1CG291900 Rh1DG305400
rosa_wichuraiana Rw1G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 440
AccIII TCCGGA 1 cut(s) 1128
AciI CCGC 4 cut(s) 159, 170, 218, 910
AclWI GGATC 4 cut(s) 368, 1110, 1120, 1156
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 3 cut(s) 497, 941, 1079
AfaI GTAC 2 cut(s) 13, 432
AfiI CCNNNNNNNGG 4 cut(s) 440, 484, 626, 840
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 9 cut(s) 47, 112, 188, 239, 502, 674, 790, 1009, 1089
AhlI ACTAGT 2 cut(s) 8, 1174
AjnI CCWGG 2 cut(s) 625, 1164
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 7 cut(s) 135, 224, 309, 317, 793, 916, 1144
AluI AGCT 7 cut(s) 135, 224, 309, 317, 793, 916, 1144
Alw21I GWGCWC 2 cut(s) 1117, 1146
Alw26I GTCTC 1 cut(s) 1215
AlwI GGATC 4 cut(s) 368, 1110, 1120, 1156
Aor13HI TCCGGA 1 cut(s) 1128
AoxI GGCC 4 cut(s) 207, 443, 559, 727
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 3 cut(s) 497, 941, 1079
Asp700I GAANNNNTTC 1 cut(s) 1020
AspS9I GGNCC 3 cut(s) 443, 559, 727
AsuHPI GGTGA 2 cut(s) 669, 670
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BalI TGGCCA 1 cut(s) 209
BanII GRGCYC 1 cut(s) 1146
Bbv12I GWGCWC 2 cut(s) 1117, 1146
BbvCI CCTCAGC 1 cut(s) 820
BbvI GCAGC 1 cut(s) 326
BccI CCATC 5 cut(s) 238, 434, 545, 833, 1069
BciT130I CCWGG 2 cut(s) 627, 1166
BcoDI GTCTC 1 cut(s) 1215
BcuI ACTAGT 2 cut(s) 8, 1174
BfaI CTAG 5 cut(s) 9, 278, 611, 990, 1175
BisI GCNGC 1 cut(s) 315
BlpI GCTNAGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 316
Bme1390I CCNGG 2 cut(s) 627, 1166
BmgT120I GGNCC 3 cut(s) 443, 559, 727
BmiI GGNNCC 1 cut(s) 1170
BmrFI CCNGG 2 cut(s) 627, 1166
BmrI ACTGGG 1 cut(s) 608
BmsI GCATC 7 cut(s) 358, 439, 558, 757, 779, 875, 1098
BmuI ACTGGG 1 cut(s) 608
BpmI CTGGAG 3 cut(s) 438, 488, 882
Bpu10I CCTNAGC 2 cut(s) 405, 820
Bpu1102I GCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 409
BsaAI YACGTR 1 cut(s) 15
BsaJI CCNNGG 3 cut(s) 562, 723, 963
BsaWI WCCGGW 1 cut(s) 1128
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 4 cut(s) 440, 484, 626, 840
Bse1I ACTGG 3 cut(s) 421, 434, 603
Bse3DI GCAATG 2 cut(s) 69, 734
BseAI TCCGGA 1 cut(s) 1128
BseBI CCWGG 2 cut(s) 627, 1166
BseDI CCNNGG 3 cut(s) 562, 723, 963
BseGI GGATG 6 cut(s) 349, 556, 573, 640, 772, 903
BseLI CCNNNNNNNGG 4 cut(s) 440, 484, 626, 840
BseMI GCAATG 2 cut(s) 69, 734
BseMII CTCAG 6 cut(s) 272, 419, 471, 567, 834, 1083
BseNI ACTGG 3 cut(s) 421, 434, 603
BseRI GAGGAG 2 cut(s) 612, 1209
BseXI GCAGC 1 cut(s) 326
BseYI CCCAGC 2 cut(s) 230, 251
BsgI GTGCAG 2 cut(s) 333, 702
BshFI GGCC 4 cut(s) 209, 445, 561, 729
BsiHKAI GWGCWC 2 cut(s) 1117, 1146
BsiSI CCGG 1 cut(s) 1129
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 4 cut(s) 440, 484, 626, 840
BsmAI GTCTC 1 cut(s) 1215
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 4 cut(s) 209, 445, 561, 729
Bsp1286I GDGCHC 2 cut(s) 1117, 1146
Bsp13I TCCGGA 1 cut(s) 1128
Bsp143I GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
Bsp1720I GCTNAGC 1 cut(s) 576
BspACI CCGC 4 cut(s) 159, 170, 218, 910
BspANI GGCC 4 cut(s) 209, 445, 561, 729
BspCNI CTCAG 6 cut(s) 271, 418, 472, 568, 833, 1082
BspEI TCCGGA 1 cut(s) 1128
BspLI GGNNCC 1 cut(s) 1170
BspPI GGATC 4 cut(s) 368, 1110, 1120, 1156
BsrDI GCAATG 2 cut(s) 69, 734
BsrI ACTGG 3 cut(s) 421, 434, 603
BssECI CCNNGG 3 cut(s) 562, 723, 963
BssMI GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 627, 1166
Bst4CI ACNGT 2 cut(s) 967, 1021
Bst6I CTCTTC 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 573, 999, 1113, 1203
BstDEI CTNAG 8 cut(s) 258, 405, 480, 576, 654, 794, 820, 1069
BstDSI CCRYGG 2 cut(s) 723, 963
BstF5I GGATG 6 cut(s) 349, 556, 573, 640, 772, 903
BstKTI GATC 5 cut(s) 376, 1105, 1128, 1164, 1191
BstMAI GTCTC 1 cut(s) 1215
BstMBI GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
BstMWI GCNNNNNNNGC 4 cut(s) 215, 323, 577, 821
BstNI CCWGG 2 cut(s) 627, 1166
BstNSI RCATGY 3 cut(s) 277, 694, 1001
BstSCI CCNGG 2 cut(s) 625, 1164
BstV1I GCAGC 1 cut(s) 326
BstXI CCANNNNNNTGG 3 cut(s) 76, 361, 970
BsuRI GGCC 4 cut(s) 209, 445, 561, 729
BtgI CCRYGG 2 cut(s) 723, 963
BtsCI GGATG 6 cut(s) 349, 556, 573, 640, 772, 903
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 3 cut(s) 210, 369, 985
Cac8I GCNNGC 4 cut(s) 573, 999, 1113, 1203
Cfr13I GGNCC 3 cut(s) 443, 559, 727
Csp6I GTAC 2 cut(s) 12, 431
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviAII CATG 6 cut(s) 274, 691, 970, 998, 1183, 1225
CviQI GTAC 2 cut(s) 12, 431
DdeI CTNAG 8 cut(s) 258, 405, 480, 576, 654, 794, 820, 1069
DpnI GATC 5 cut(s) 375, 1104, 1127, 1163, 1190
DpnII GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 1144
Eco130I CCWWGG 1 cut(s) 562
Eco24I GRGCYC 1 cut(s) 1146
Eco32I GATATC 1 cut(s) 807
Eco53kI GAGCTC 1 cut(s) 1144
EcoICRI GAGCTC 1 cut(s) 1144
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 2 cut(s) 625, 1164
EcoRV GATATC 1 cut(s) 807
EcoT14I CCWWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 772
EcoT38I GRGCYC 1 cut(s) 1146
ErhI CCWWGG 1 cut(s) 562
FaeI CATG 6 cut(s) 277, 694, 973, 1001, 1186, 1228
FaqI GGGAC 1 cut(s) 268
FatI CATG 6 cut(s) 273, 690, 969, 997, 1182, 1224
FauI CCCGC 1 cut(s) 903
Fnu4HI GCNGC 1 cut(s) 315
FokI GGATG 6 cut(s) 336, 563, 580, 647, 779, 910
FriOI GRGCYC 1 cut(s) 1146
Fsp4HI GCNGC 1 cut(s) 315
FspBI CTAG 5 cut(s) 9, 278, 611, 990, 1175
GluI GCNGC 1 cut(s) 315
GsaI CCCAGC 2 cut(s) 234, 255
GsuI CTGGAG 3 cut(s) 438, 488, 882
HaeIII GGCC 4 cut(s) 209, 445, 561, 729
HapII CCGG 1 cut(s) 1129
Hin1II CATG 6 cut(s) 277, 694, 973, 1001, 1186, 1228
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 6 cut(s) 256, 395, 674, 932, 983, 1051
HpaII CCGG 1 cut(s) 1129
HphI GGTGA 2 cut(s) 669, 670
Hpy166II GTNNAC 3 cut(s) 149, 696, 1180
Hpy188I TCNGA 7 cut(s) 516, 871, 1050, 1072, 1107, 1150, 1193
Hpy188III TCNNGA 8 cut(s) 60, 407, 467, 671, 861, 923, 1118, 1129
Hpy8I GTNNAC 3 cut(s) 149, 696, 1180
HpyAV CCTTC 4 cut(s) 40, 478, 1006, 1217
HpyCH4III ACNGT 2 cut(s) 967, 1021
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 6 cut(s) 314, 509, 719, 770, 997, 1077
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 323, 577, 821
HpyF3I CTNAG 8 cut(s) 258, 405, 480, 576, 654, 794, 820, 1069
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 6 cut(s) 277, 694, 973, 1001, 1186, 1228
Kpn2I TCCGGA 1 cut(s) 1128
Kzo9I GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
LmnI GCTCC 5 cut(s) 103, 457, 863, 913, 1174
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 7 cut(s) 358, 439, 558, 757, 779, 875, 1098
MaeI CTAG 5 cut(s) 9, 278, 611, 990, 1175
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 952
MalI GATC 5 cut(s) 375, 1104, 1127, 1163, 1190
MboI GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
MboII GAAGA 6 cut(s) 35, 251, 506, 801, 1021, 1046
MfeI CAATTG 2 cut(s) 642, 774
MhlI GDGCHC 2 cut(s) 1117, 1146
MlsI TGGCCA 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 250, 992
MmeI TCCRAC 1 cut(s) 277
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 772
MroI TCCGGA 1 cut(s) 1128
MroXI GAANNNNTTC 1 cut(s) 1020
MscI TGGCCA 1 cut(s) 209
MseI TTAA 3 cut(s) 593, 903, 1044
MslI CAYNNNNRTG 3 cut(s) 74, 359, 968
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 1 cut(s) 1129
MspR9I CCNGG 2 cut(s) 627, 1166
MunI CAATTG 2 cut(s) 642, 774
MvaI CCWGG 2 cut(s) 627, 1166
MwoI GCNNNNNNNGC 4 cut(s) 215, 323, 577, 821
NdeII GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
NlaIII CATG 6 cut(s) 277, 694, 973, 1001, 1186, 1228
NlaIV GGNNCC 1 cut(s) 1170
NsiI ATGCAT 1 cut(s) 772
NspI RCATGY 3 cut(s) 277, 694, 1001
OliI CACNNNNGTG 1 cut(s) 359
PaeI GCATGC 1 cut(s) 1001
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 4 cut(s) 395, 674, 932, 1051
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 1 cut(s) 316
PleI GAGTC 2 cut(s) 250, 991
PpsI GAGTC 2 cut(s) 250, 991
Ppu21I YACGTR 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 1146
Psp6I CCWGG 2 cut(s) 625, 1164
PspFI CCCAGC 2 cut(s) 230, 251
PspGI CCWGG 2 cut(s) 625, 1164
PspN4I GGNNCC 1 cut(s) 1170
PspPI GGNCC 3 cut(s) 443, 559, 727
RsaI GTAC 2 cut(s) 13, 432
RsaNI GTAC 2 cut(s) 12, 431
RseI CAYNNNNRTG 3 cut(s) 74, 359, 968
SacI GAGCTC 1 cut(s) 1146
SaqAI TTAA 3 cut(s) 593, 903, 1044
SatI GCNGC 1 cut(s) 315
Sau3AI GATC 5 cut(s) 373, 1102, 1125, 1161, 1188
Sau96I GGNCC 3 cut(s) 443, 559, 727
SchI GAGTC 2 cut(s) 250, 992
ScrFI CCNGG 2 cut(s) 627, 1166
SduI GDGCHC 2 cut(s) 1117, 1146
SfaNI GCATC 7 cut(s) 358, 439, 558, 757, 779, 875, 1098
SmiMI CAYNNNNRTG 3 cut(s) 74, 359, 968
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SpeI ACTAGT 2 cut(s) 8, 1174
SphI GCATGC 1 cut(s) 1001
SsiI CCGC 4 cut(s) 159, 170, 218, 910
SspMI CTAG 5 cut(s) 9, 278, 611, 990, 1175
SstI GAGCTC 1 cut(s) 1146
StyD4I CCNGG 2 cut(s) 625, 1164
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 2 cut(s) 967, 1021
TaiI ACGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 1210
TfiI GAWTC 4 cut(s) 395, 674, 932, 1051
Tru1I TTAA 3 cut(s) 593, 903, 1044
Tru9I TTAA 3 cut(s) 593, 903, 1044
TscAI CASTG 3 cut(s) 210, 376, 985
TseI GCWGC 1 cut(s) 314
TspDTI ATGAA 1 cut(s) 651
TspGWI ACGGA 1 cut(s) 823
TspRI CASTG 3 cut(s) 210, 376, 985
Van91I CCANNNNNTGG 1 cut(s) 440
XapI RAATTY 3 cut(s) 497, 941, 1079
XceI RCATGY 3 cut(s) 277, 694, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 1020
XspI CTAG 5 cut(s) 9, 278, 611, 990, 1175
Zsp2I ATGCAT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.