MD07G1171700.v1.1

Arsenical pump-driving

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010871
Physical Location & Seq
Reverse (-)
24865367 .. 24870018
4652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1171700.v1.1.491

Sequence Viewer

Length: 1338 bp
ATGGTTTACGGGTCAAGTACTCGAGCTCCATCCGGCTACAATCCGAGTTTATATAAATACCAACCGACCAACGGAAATTGCTCTAAAACTCCAACCAGAAAAACCATGGCGAGTAGCACATGTATTCCCTCCACTTTCACACCAATTCTTCACAACTTGACCGCCAGAAACTCCATGACAATGGTGGGTTTGCTATCTTATGCTCCCAAAACCCTCAGACCTCTCTCTCTCCCCAAAAGCTTCGGTTTCAGCTCACTTTCCACCGCCGCAAAACTCCCCAGAAAATTCTTCCAATTGCAAGTGAGATCAGTGGCCACTCCAGCAGAAGCTGTTGCTGGGTTTGATGACATGGTTGCTGGGACTCAGAGAAAGTATTACATGTTAGGCGGGAAGGGCGGTGTAGGGAAGACAAGTTGTGCTGCTTCACTTGCCGTAAAATTCGCTAACAATGGGCATCCCACTCTTGTGGTTTCCACTGATCCAGCACATTCCTTGAGTGATTCCTTTGCCCAGGATTTGGCTGGGGGGACACTTGTACCAGTTGATGGGCCTGAAGCTCCACTTTTTGCTCTTGAGATAAACCCGGAGAAGGCAAGGGAAGAATTCCGTAATGTAAGTCAGAAAAATGATGGAACAGGAGTCAAAGATTTCATGGATGGTATGGGCCTTGGGATGCTTGCGGAACAGTTAGGAGATTTAAAATTGGGAGAATTACTAGACACACCTCCTCCTGGTTTGGATGAAGCTCTAGCAATTTCCAAAGTGATACAATTTCTTGAAGCCCCAGAATATAGCATGTTTACCCGCATAGTTTTTGATACTGCACCCACGGGTCACACATTGCGGCTTTTATCATTGCCAGACTTTTTGGATCAATCAATTGGCAAGATATTGCAGCTTAAACAAAAGATATCTTCAGCCACCTCAGCTATCAAATCTGTTTTTGGGCAAGAGCAACCCCAAATGGGCGCGGCTGACAAATTAGAGAAACTGAGGGAAAGGATGATTAAAGTGCGTGAACTTTTTCATGACACTGATTCAACAGAGTTTGTCATAGTAACAATACCTACGGTGATGGCCGTTAGTGAGTCATCTAGGTTGCATGCCTCTCTGAAGAAGGAAAGCGTACCTGTGACTCGACTTATTGTTAATCAGATTCTTCCCCCATCCGCCTCAGATTGCAAATTTTGTTCAATTAAAAGAAAGATGCGTGCTCTTGATATGATACAGAAGGATACAGAGCTCTCTGGCTTGACAGTTATCCAGGCTCCACTAGTTGATGTGGAGATCAGAGGCGTTCCTGCCCTTAGATTTCTTGGAGACATAATTTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

446

Amino Acids

48.23

Weight (kDa)

9.0

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArsA_ATPase PF02374 124 - 443 6e-71 Anion-transporting ATPase
CbiA PF01656 128 - 360 3.9e-08 CobQ/CobB/MinD/ParA nucleotide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10350
fragaria_vesca FvH4_7g18990 FvH4_7g18990
malus_domestica MD01G1105500.v1.1 MD07G1171700.v1.1
prunus_persica Prupe.2G211000_v2.0.a1 Prupe.2G211000_v2.0.a1 Prupe.2G211000_v2.0.a1
pyrus_communis pycom01g13230 pycom07g16630
rosa_chinensis RchiOBHm_Chr1g0362921
rosa_laevigata RLG00000027612
rosa_multiflora Rmu_sc0001502.1_g000006
rosa_roxburghii Rroxscaffold_4G00293650
rosa_rugosa Rorug01G0302100
rosa_samantha Rh1AG311600 Rh1BG274700 Rh1CG291900 Rh1DG305400
rosa_wichuraiana Rw1G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 517, 545
AccII CGCG 1 cut(s) 971
AclWI GGATC 2 cut(s) 473, 879
AcoI YGGCCR 2 cut(s) 312, 1077
AcsI RAATTY 4 cut(s) 284, 437, 602, 1184
AcuI CTGAAG 3 cut(s) 573, 900, 1133
AfaI GTAC 3 cut(s) 19, 537, 1128
AfiI CCNNNNNNNGG 6 cut(s) 71, 517, 545, 589, 731, 965
AflIII ACRYGT 2 cut(s) 119, 378
AgsI TTSAA 3 cut(s) 779, 1041, 1194
AhlI ACTAGT 1 cut(s) 1273
AjnI CCWGG 3 cut(s) 510, 730, 1263
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 9 cut(s) 26, 240, 252, 329, 557, 746, 898, 929, 1243
AluI AGCT 9 cut(s) 26, 240, 252, 329, 557, 746, 898, 929, 1243
Alw21I GWGCWC 3 cut(s) 28, 1216, 1245
Alw26I GTCTC 1 cut(s) 1314
AlwI GGATC 2 cut(s) 473, 879
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 21
AoxI GGCC 4 cut(s) 312, 548, 664, 1077
ApeKI GCWGC 2 cut(s) 419, 895
ApoI RAATTY 4 cut(s) 284, 437, 602, 1184
Asp700I GAANNNNTTC 1 cut(s) 1023
AspLEI GCGC 1 cut(s) 971
AspS9I GGNCC 2 cut(s) 548, 664
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 1084
AvaI CYCGRG 1 cut(s) 21
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BalI TGGCCA 1 cut(s) 314
BanII GRGCYC 2 cut(s) 28, 1245
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 3 cut(s) 28, 1216, 1245
BbvCI CCTCAGC 1 cut(s) 925
BbvI GCAGC 2 cut(s) 406, 907
BccI CCATC 6 cut(s) 37, 539, 623, 650, 1069, 1174
BceAI ACGGC 2 cut(s) 416, 1064
BciT130I CCWGG 3 cut(s) 512, 732, 1265
BciVI GTATCC 1 cut(s) 1228
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 1 cut(s) 1314
BcuI ACTAGT 1 cut(s) 1273
BfaI CTAG 4 cut(s) 716, 749, 1095, 1274
BfuI GTATCC 1 cut(s) 1228
BisI GCNGC 5 cut(s) 267, 420, 845, 896, 972
BlsI GCNGC 5 cut(s) 268, 421, 846, 897, 973
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 4 cut(s) 512, 584, 732, 1265
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 548, 664
BmiI GGNNCC 1 cut(s) 1269
BmrFI CCNGG 4 cut(s) 512, 584, 732, 1265
BmsI GCATC 3 cut(s) 463, 663, 1197
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 303
Bpu10I CCTNAGC 1 cut(s) 925
BpuEI CTTGAG 2 cut(s) 514, 593
BpuMI CCSGG 1 cut(s) 584
BsaJI CCNNGG 4 cut(s) 105, 510, 667, 828
BsaXI ACNNNNNCTCC 4 cut(s) 10, 40, 712, 742
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 517, 545, 589, 731, 965
Bse1I ACTGG 1 cut(s) 539
Bse3DI GCAATG 2 cut(s) 839, 854
BseBI CCWGG 3 cut(s) 512, 732, 1265
BseDI CCNNGG 4 cut(s) 105, 510, 667, 828
BseGI GGATG 7 cut(s) 29, 454, 661, 678, 745, 1008, 1166
BseLI CCNNNNNNNGG 6 cut(s) 71, 517, 545, 589, 731, 965
BseMI GCAATG 2 cut(s) 839, 854
BseMII CTCAG 5 cut(s) 229, 377, 939, 983, 1188
BseNI ACTGG 1 cut(s) 539
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 2 cut(s) 406, 907
BseYI CCCAGC 3 cut(s) 335, 356, 521
BsgI GTGCAG 1 cut(s) 807
Bsh1236I CGCG 1 cut(s) 971
BshFI GGCC 4 cut(s) 314, 550, 666, 1079
BsiHKAI GWGCWC 3 cut(s) 28, 1216, 1245
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 2 cut(s) 33, 584
BslFI GGGAC 2 cut(s) 373, 541
BslI CCNNNNNNNGG 6 cut(s) 71, 517, 545, 589, 731, 965
BsmAI GTCTC 1 cut(s) 1314
BsmFI GGGAC 2 cut(s) 373, 541
BsnI GGCC 4 cut(s) 314, 550, 666, 1079
BsoBI CYCGRG 1 cut(s) 21
Bsp1286I GDGCHC 3 cut(s) 28, 1216, 1245
Bsp143I GATC 4 cut(s) 305, 478, 871, 1287
Bsp19I CCATGG 1 cut(s) 105
BspANI GGCC 4 cut(s) 314, 550, 666, 1079
BspCNI CTCAG 5 cut(s) 228, 376, 938, 984, 1187
BspFNI CGCG 1 cut(s) 971
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 1 cut(s) 1269
BspPI GGATC 2 cut(s) 473, 879
BsrDI GCAATG 2 cut(s) 839, 854
BsrI ACTGG 1 cut(s) 539
BssECI CCNNGG 4 cut(s) 105, 510, 667, 828
BssMI GATC 4 cut(s) 305, 478, 871, 1287
BssT1I CCWWGG 2 cut(s) 105, 667
Bst2UI CCWGG 3 cut(s) 512, 732, 1265
Bst4CI ACNGT 3 cut(s) 687, 1072, 1258
BstC8I GCNNGC 3 cut(s) 678, 1104, 1212
BstDEI CTNAG 6 cut(s) 215, 363, 925, 992, 1174, 1307
BstDSI CCRYGG 2 cut(s) 105, 828
BstF5I GGATG 7 cut(s) 29, 454, 661, 678, 745, 1008, 1166
BstFNI CGCG 1 cut(s) 971
BstHHI GCGC 1 cut(s) 971
BstKTI GATC 4 cut(s) 308, 481, 874, 1290
BstMAI GTCTC 1 cut(s) 1314
BstMBI GATC 4 cut(s) 305, 478, 871, 1287
BstMWI GCNNNNNNNGC 4 cut(s) 320, 393, 428, 926
BstNI CCWGG 3 cut(s) 512, 732, 1265
BstNSI RCATGY 4 cut(s) 123, 382, 799, 1106
BstSCI CCNGG 4 cut(s) 510, 582, 730, 1263
BstUI CGCG 1 cut(s) 971
BstV1I GCAGC 2 cut(s) 406, 907
BstV2I GAAGAC 1 cut(s) 413
BstXI CCANNNNNNTGG 2 cut(s) 181, 466
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 4 cut(s) 314, 550, 666, 1079
BtgI CCRYGG 2 cut(s) 105, 828
BtsCI GGATG 7 cut(s) 29, 454, 661, 678, 745, 1008, 1166
BtsIMutI CAGTG 3 cut(s) 315, 474, 1032
Cac8I GCNNGC 3 cut(s) 678, 1104, 1212
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 1 cut(s) 971
Cfr13I GGNCC 2 cut(s) 548, 664
Csp6I GTAC 3 cut(s) 18, 536, 1127
CviAII CATG 9 cut(s) 106, 120, 175, 349, 379, 652, 796, 1028, 1103
CviQI GTAC 3 cut(s) 18, 536, 1127
DdeI CTNAG 6 cut(s) 215, 363, 925, 992, 1174, 1307
DpnI GATC 4 cut(s) 307, 480, 873, 1289
DpnII GATC 4 cut(s) 305, 478, 871, 1287
DraI TTTAAA 1 cut(s) 699
EaeI YGGCCR 2 cut(s) 312, 1077
EciI GGCGGA 1 cut(s) 1159
Ecl136II GAGCTC 2 cut(s) 26, 1243
Eco130I CCWWGG 2 cut(s) 105, 667
Eco24I GRGCYC 2 cut(s) 28, 1245
Eco32I GATATC 1 cut(s) 912
Eco53kI GAGCTC 2 cut(s) 26, 1243
Eco57I CTGAAG 3 cut(s) 573, 900, 1133
Eco88I CYCGRG 1 cut(s) 21
EcoICRI GAGCTC 2 cut(s) 26, 1243
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 3 cut(s) 510, 730, 1263
EcoRV GATATC 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 105, 667
EcoT38I GRGCYC 2 cut(s) 28, 1245
ErhI CCWWGG 2 cut(s) 105, 667
FaeI CATG 9 cut(s) 109, 123, 178, 352, 382, 655, 799, 1031, 1106
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 2 cut(s) 373, 541
FatI CATG 9 cut(s) 105, 119, 174, 348, 378, 651, 795, 1027, 1102
FauI CCCGC 2 cut(s) 380, 812
Fnu4HI GCNGC 5 cut(s) 267, 420, 845, 896, 972
FokI GGATG 7 cut(s) 16, 441, 668, 685, 752, 1015, 1153
FriOI GRGCYC 2 cut(s) 28, 1245
Fsp4HI GCNGC 5 cut(s) 267, 420, 845, 896, 972
FspBI CTAG 4 cut(s) 716, 749, 1095, 1274
GlaI GCGC 1 cut(s) 970
GluI GCNGC 5 cut(s) 267, 420, 845, 896, 972
GsaI CCCAGC 3 cut(s) 339, 360, 525
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 4 cut(s) 314, 550, 666, 1079
HapII CCGG 2 cut(s) 33, 584
HhaI GCGC 1 cut(s) 971
Hin1II CATG 9 cut(s) 109, 123, 178, 352, 382, 655, 799, 1031, 1106
Hin6I GCGC 1 cut(s) 969
HinP1I GCGC 1 cut(s) 969
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 7 cut(s) 361, 500, 639, 1037, 1088, 1135, 1156
HpaII CCGG 2 cut(s) 33, 584
HphI GGTGA 1 cut(s) 1084
Hpy166II GTNNAC 3 cut(s) 7, 801, 1019
Hpy188I TCNGA 8 cut(s) 45, 218, 366, 621, 1113, 1155, 1177, 1292
Hpy188III TCNNGA 4 cut(s) 572, 776, 1028, 1217
Hpy8I GTNNAC 3 cut(s) 7, 801, 1019
HpyAV CCTTC 4 cut(s) 385, 583, 1111, 1225
HpyCH4III ACNGT 3 cut(s) 687, 1072, 1258
HpyCH4V TGCA 5 cut(s) 298, 824, 895, 1102, 1182
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 393, 428, 926
HpyF3I CTNAG 6 cut(s) 215, 363, 925, 992, 1174, 1307
Hsp92II CATG 9 cut(s) 109, 123, 178, 352, 382, 655, 799, 1031, 1106
HspAI GCGC 1 cut(s) 969
Kzo9I GATC 4 cut(s) 305, 478, 871, 1287
LmnI GCTCC 4 cut(s) 31, 208, 562, 1273
Lsp1109I GCAGC 2 cut(s) 406, 907
LweI GCATC 3 cut(s) 463, 663, 1197
MaeI CTAG 4 cut(s) 716, 749, 1095, 1274
MaeIII GTNAC 3 cut(s) 833, 1057, 1132
MalI GATC 4 cut(s) 307, 480, 873, 1289
MboI GATC 4 cut(s) 305, 478, 871, 1287
MboII GAAGA 7 cut(s) 140, 280, 418, 611, 906, 1126, 1151
MfeI CAATTG 2 cut(s) 293, 879
MhlI GDGCHC 3 cut(s) 28, 1216, 1245
MlsI TGGCCA 1 cut(s) 314
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 4 cut(s) 355, 648, 1097, 1129
MmeI TCCRAC 1 cut(s) 116
Mox20I TGGCCA 1 cut(s) 314
MroXI GAANNNNTTC 1 cut(s) 1023
MscI TGGCCA 1 cut(s) 314
MseI TTAA 5 cut(s) 698, 900, 1008, 1149, 1197
MslI CAYNNNNRTG 2 cut(s) 179, 464
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 2 cut(s) 33, 584
MspR9I CCNGG 4 cut(s) 512, 584, 732, 1265
MunI CAATTG 2 cut(s) 293, 879
MvaI CCWGG 3 cut(s) 512, 732, 1265
MvnI CGCG 1 cut(s) 971
MwoI GCNNNNNNNGC 4 cut(s) 320, 393, 428, 926
NciI CCSGG 1 cut(s) 584
NcoI CCATGG 1 cut(s) 105
NdeII GATC 4 cut(s) 305, 478, 871, 1287
NlaIII CATG 9 cut(s) 109, 123, 178, 352, 382, 655, 799, 1031, 1106
NlaIV GGNNCC 1 cut(s) 1269
NmuCI GTSAC 2 cut(s) 833, 1132
NspI RCATGY 4 cut(s) 123, 382, 799, 1106
OliI CACNNNNGTG 1 cut(s) 464
PaeI GCATGC 1 cut(s) 1106
PaeR7I CTCGAG 1 cut(s) 21
PagI TCATGA 1 cut(s) 1027
PciI ACATGT 2 cut(s) 119, 378
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 3 cut(s) 500, 1037, 1156
PflMI CCANNNNNTGG 2 cut(s) 517, 545
PkrI GCNGC 5 cut(s) 268, 421, 846, 897, 973
PleI GAGTC 4 cut(s) 355, 647, 1096, 1129
PpsI GAGTC 4 cut(s) 355, 647, 1096, 1129
PscI ACATGT 2 cut(s) 119, 378
Psp124BI GAGCTC 2 cut(s) 28, 1245
Psp6I CCWGG 3 cut(s) 510, 730, 1263
PspFI CCCAGC 3 cut(s) 335, 356, 521
PspGI CCWGG 3 cut(s) 510, 730, 1263
PspN4I GGNNCC 1 cut(s) 1269
PspPI GGNCC 2 cut(s) 548, 664
PspXI VCTCGAGB 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 329
RsaI GTAC 3 cut(s) 19, 537, 1128
RsaNI GTAC 3 cut(s) 18, 536, 1127
RseI CAYNNNNRTG 2 cut(s) 179, 464
SacI GAGCTC 2 cut(s) 28, 1245
SaqAI TTAA 5 cut(s) 698, 900, 1008, 1149, 1197
SatI GCNGC 5 cut(s) 267, 420, 845, 896, 972
Sau3AI GATC 4 cut(s) 305, 478, 871, 1287
Sau96I GGNCC 2 cut(s) 548, 664
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 4 cut(s) 355, 648, 1097, 1129
ScrFI CCNGG 4 cut(s) 512, 584, 732, 1265
SduI GDGCHC 3 cut(s) 28, 1216, 1245
SfaNI GCATC 3 cut(s) 463, 663, 1197
Sfr274I CTCGAG 1 cut(s) 21
SlaI CTCGAG 1 cut(s) 21
SmiMI CAYNNNNRTG 2 cut(s) 179, 464
SmlI CTYRAG 3 cut(s) 21, 493, 572
SmoI CTYRAG 3 cut(s) 21, 493, 572
SpeI ACTAGT 1 cut(s) 1273
SphI GCATGC 1 cut(s) 1106
SspMI CTAG 4 cut(s) 716, 749, 1095, 1274
SstI GAGCTC 2 cut(s) 28, 1245
StyD4I CCNGG 4 cut(s) 510, 582, 730, 1263
StyI CCWWGG 2 cut(s) 105, 667
TaaI ACNGT 3 cut(s) 687, 1072, 1258
TaqI TCGA 2 cut(s) 22, 1138
TatI WGTACW 1 cut(s) 17
TauI GCSGC 3 cut(s) 269, 847, 974
TfiI GAWTC 3 cut(s) 500, 1037, 1156
Tru1I TTAA 5 cut(s) 698, 900, 1008, 1149, 1197
Tru9I TTAA 5 cut(s) 698, 900, 1008, 1149, 1197
TscAI CASTG 3 cut(s) 315, 481, 1039
TseFI GTSAC 2 cut(s) 833, 1132
TseI GCWGC 2 cut(s) 419, 895
Tsp45I GTSAC 2 cut(s) 833, 1132
TspDTI ATGAA 3 cut(s) 640, 756, 1016
TspGWI ACGGA 2 cut(s) 87, 596
TspRI CASTG 3 cut(s) 315, 481, 1039
Van91I CCANNNNNTGG 2 cut(s) 517, 545
XapI RAATTY 4 cut(s) 284, 437, 602, 1184
XceI RCATGY 4 cut(s) 123, 382, 799, 1106
XcmI CCANNNNNNNNNTGG 3 cut(s) 103, 181, 518
XhoI CTCGAG 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 4 cut(s) 716, 749, 1095, 1274
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.