MD01G1111500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016924
Physical Location & Seq
Reverse (-)
22420490 .. 22421119
630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1111500.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGAAACTTTCTCAGATTTTCAGCAGCAAGAAGTTGATGAAGATGCCCTTTCCCTCTGCGATCTCCCGCTGTACGACGACAATGGCGAATCGGAAGAGAACCCAATAACCTCCGCTTCTTATTCTTCCGCCCCGGACGACCTTTTTGAATTTCGGGTCGACCCGATCCGGAGATACATCCCCATGGACGTCTTTTTTTGCGGAAAACCCATTTCCCCTCGGACCCAAGAGCCCCTATTCGAAAACCCATTGTTCTCCCGGTCCGAATCCTTGAAGTTTTCATCTCCCTCCCACCCTTACTCGCACCGGTCACCGGGATCGGAGTTGGTGCCAGCTTTTGGGACCTGCCGGTCGCACCAGCAGTCGAGCAATTCGCGGAAGCACAAGGTGTTCATAGGATTGGTGAAGTACCAGCCGGAGATGGACATGAATGAGATTAGGAAGCGGCAGAGTCGGCGCGCTCCGGCGCCGATGTTTCCGGTGATAAGCAGCGTAGAGCAACCGAGTGTAGCCTGTGGGAAGAGTGGGTCCGGGAAAGGGCACTGGGGGCTGATGAGGCCGCTGAGGTGTCGGTTCCACTTGTTAGGCGCATTGGCTAAGGTGACTCATGGTTGTGTGCCACGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.55

Weight (kDa)

7.04

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016924)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20010
malus_domestica MD01G1111500.v1.1 MD07G1177700.v1.1
prunus_persica Prupe.2G217100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363961
rosa_laevigata RLG00000027532
rosa_multiflora Rmu_ssc0000167.1_g000035
rosa_roxburghii Rroxscaffold_4G00292800
rosa_rugosa Rorug01G0311700
rosa_samantha Rh1AG319500 Rh1BG282600 Rh1CG299000 Rh1DG314000
rosa_wichuraiana Rw1G028320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 2 cut(s) 328, 466
AccB7I CCANNNNNTGG 1 cut(s) 338
AccI GTMKAC 1 cut(s) 159
AccII CGCG 2 cut(s) 376, 459
AccIII TCCGGA 1 cut(s) 168
AciI CCGC 7 cut(s) 68, 114, 129, 201, 376, 445, 560
AclWI GGATC 2 cut(s) 160, 325
AcsI RAATTY 1 cut(s) 149
AcvI CACGTG 1 cut(s) 623
AcyI GRCGYC 2 cut(s) 189, 467
AdeI CACNNNGTG 1 cut(s) 388
AfaI GTAC 2 cut(s) 74, 410
AfiI CCNNNNNNNGG 3 cut(s) 313, 338, 537
AflIII ACRYGT 1 cut(s) 622
AgeI ACCGGT 1 cut(s) 306
AgsI TTSAA 2 cut(s) 149, 274
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
AlwI GGATC 2 cut(s) 160, 325
Aor13HI TCCGGA 1 cut(s) 168
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 2 cut(s) 25, 489
ApoI RAATTY 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AsiGI ACCGGT 1 cut(s) 306
AspLEI GCGC 4 cut(s) 459, 461, 469, 590
AspS9I GGNCC 4 cut(s) 222, 261, 342, 528
AsuC2I CCSGG 4 cut(s) 134, 259, 315, 532
AsuHPI GGTGA 4 cut(s) 303, 415, 493, 613
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 4 cut(s) 222, 261, 342, 528
BaeGI GKGCMC 1 cut(s) 543
BanI GGYRCC 2 cut(s) 328, 466
BanII GRGCYC 1 cut(s) 234
BbrPI CACGTG 1 cut(s) 623
BbvCI CCTCAGC 1 cut(s) 563
BbvI GCAGC 2 cut(s) 37, 501
BccI CCATC 1 cut(s) 415
BcnI CCSGG 4 cut(s) 134, 259, 315, 532
BfoI RGCGCY 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 353
BisI GCNGC 4 cut(s) 26, 446, 490, 560
BlsI GCNGC 4 cut(s) 27, 447, 491, 561
Bme1390I CCNGG 4 cut(s) 134, 259, 315, 532
Bme18I GGWCC 4 cut(s) 222, 261, 342, 528
BmgT120I GGNCC 4 cut(s) 222, 261, 342, 528
BmiI GGNNCC 6 cut(s) 224, 330, 343, 468, 529, 575
BmrFI CCNGG 4 cut(s) 134, 259, 315, 532
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 1 cut(s) 34
BmuI ACTGGG 1 cut(s) 553
Bpu10I CCTNAGC 2 cut(s) 563, 597
Bpu14I TTCGAA 1 cut(s) 240
BpuMI CCSGG 4 cut(s) 134, 259, 315, 532
BsaAI YACGTR 1 cut(s) 623
BsaHI GRCGYC 2 cut(s) 189, 467
BsaJI CCNNGG 3 cut(s) 132, 183, 218
BsaWI WCCGGW 3 cut(s) 168, 306, 478
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 338, 537
Bse118I RCCGGY 2 cut(s) 306, 348
Bse1I ACTGG 1 cut(s) 548
BseAI TCCGGA 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 132, 183, 218
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 3 cut(s) 313, 338, 537
BseMII CTCAG 2 cut(s) 27, 554
BseNI ACTGG 1 cut(s) 548
BsePI GCGCGC 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 543
BseXI GCAGC 2 cut(s) 37, 501
Bsh1236I CGCG 2 cut(s) 376, 459
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 1 cut(s) 559
BshNI GGYRCC 2 cut(s) 328, 466
BshTI ACCGGT 1 cut(s) 306
BsiEI CGRYCG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 3 cut(s) 313, 338, 537
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 1 cut(s) 559
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 234, 543
Bsp13I TCCGGA 1 cut(s) 168
Bsp143I GATC 3 cut(s) 61, 165, 317
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 7 cut(s) 68, 114, 129, 201, 376, 445, 560
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 2 cut(s) 26, 555
BspEI TCCGGA 1 cut(s) 168
BspFNI CGCG 2 cut(s) 376, 459
BspLI GGNNCC 6 cut(s) 224, 330, 343, 468, 529, 575
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 160, 325
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 2 cut(s) 328, 466
BsrFI RCCGGY 2 cut(s) 306, 348
BsrI ACTGG 1 cut(s) 548
BssAI RCCGGY 2 cut(s) 306, 348
BssECI CCNNGG 3 cut(s) 132, 183, 218
BssHII GCGCGC 1 cut(s) 457
BssMI GATC 3 cut(s) 61, 165, 317
BssNI GRCGYC 2 cut(s) 189, 467
BssT1I CCWWGG 1 cut(s) 183
Bst6I CTCTTC 2 cut(s) 90, 515
BstACI GRCGYC 2 cut(s) 189, 467
BstBAI YACGTR 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 333, 459
BstDEI CTNAG 3 cut(s) 13, 563, 597
BstDSI CCRYGG 1 cut(s) 183
BstEII GGTNACC 1 cut(s) 309
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 2 cut(s) 376, 459
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 4 cut(s) 459, 461, 469, 590
BstKTI GATC 3 cut(s) 64, 168, 320
BstMBI GATC 3 cut(s) 61, 165, 317
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 3 cut(s) 454, 547, 556
BstPI GGTNACC 1 cut(s) 309
BstSCI CCNGG 4 cut(s) 132, 257, 313, 530
BstSLI GKGCMC 1 cut(s) 543
BstUI CGCG 2 cut(s) 376, 459
BstV1I GCAGC 2 cut(s) 37, 501
BsuRI GGCC 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 541
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 333, 459
CfoI GCGC 4 cut(s) 459, 461, 469, 590
Cfr10I RCCGGY 2 cut(s) 306, 348
Cfr13I GGNCC 4 cut(s) 222, 261, 342, 528
CpoI CGGWCCG 1 cut(s) 261
Csp6I GTAC 2 cut(s) 73, 409
CspAI ACCGGT 1 cut(s) 306
CspI CGGWCCG 1 cut(s) 261
CviAII CATG 3 cut(s) 184, 427, 608
CviJI RGCY 7 cut(s) 232, 335, 415, 512, 550, 559, 596
CviKI_1 RGCY 7 cut(s) 232, 335, 415, 512, 550, 559, 596
CviQI GTAC 2 cut(s) 73, 409
DdeI CTNAG 3 cut(s) 13, 563, 597
DinI GGCGCC 1 cut(s) 468
DpnI GATC 3 cut(s) 63, 167, 319
DpnII GATC 3 cut(s) 61, 165, 317
DraIII CACNNNGTG 1 cut(s) 388
DrdI GACNNNNNNGTC 1 cut(s) 349
DseDI GACNNNNNNGTC 1 cut(s) 349
Eam1104I CTCTTC 2 cut(s) 90, 515
EarI CTCTTC 2 cut(s) 90, 515
EciI GGCGGA 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 4 cut(s) 222, 261, 342, 528
Eco72I CACGTG 1 cut(s) 623
Eco91I GGTNACC 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 342
EcoO65I GGTNACC 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 468
EheI GGCGCC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 3 cut(s) 187, 430, 611
FaiI YATR 4 cut(s) 185, 395, 428, 609
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FaqI GGGAC 1 cut(s) 355
FatI CATG 3 cut(s) 183, 426, 607
FauI CCCGC 1 cut(s) 75
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 26, 446, 490, 560
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 4 cut(s) 26, 446, 490, 560
GlaI GCGC 4 cut(s) 458, 460, 468, 589
GluI GCNGC 4 cut(s) 26, 446, 490, 560
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 1 cut(s) 559
HhaI GCGC 4 cut(s) 459, 461, 469, 590
Hin1I GRCGYC 2 cut(s) 189, 467
Hin1II CATG 3 cut(s) 187, 430, 611
Hin6I GCGC 4 cut(s) 457, 459, 467, 588
HinP1I GCGC 4 cut(s) 457, 459, 467, 588
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 4 cut(s) 89, 266, 451, 604
HphI GGTGA 4 cut(s) 303, 415, 493, 613
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 6 cut(s) 16, 94, 222, 265, 322, 629
Hpy188III TCNNGA 1 cut(s) 169
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 80
HpyCH4IV ACGT 2 cut(s) 189, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 454, 547, 556
HpyF3I CTNAG 3 cut(s) 13, 563, 597
HpySE526I ACGT 2 cut(s) 189, 622
Hsp92I GRCGYC 2 cut(s) 189, 467
Hsp92II CATG 3 cut(s) 187, 430, 611
HspAI GCGC 4 cut(s) 457, 459, 467, 588
KasI GGCGCC 1 cut(s) 466
Kpn2I TCCGGA 1 cut(s) 168
Kzo9I GATC 3 cut(s) 61, 165, 317
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 2 cut(s) 37, 501
LweI GCATC 1 cut(s) 34
MaeII ACGT 2 cut(s) 189, 622
MaeIII GTNAC 2 cut(s) 309, 601
MalI GATC 3 cut(s) 63, 167, 319
MboI GATC 3 cut(s) 61, 165, 317
MboII GAAGA 4 cut(s) 53, 107, 117, 532
MhlI GDGCHC 2 cut(s) 234, 543
MluCI AATT 2 cut(s) 149, 370
Mly113I GGCGCC 1 cut(s) 467
MlyI GAGTC 2 cut(s) 460, 598
MnlI CCTC 6 cut(s) 65, 121, 228, 298, 549, 558
MroI TCCGGA 1 cut(s) 168
MslI CAYNNNNRTG 2 cut(s) 182, 612
MspA1I CMGCKG 2 cut(s) 70, 562
MspR9I CCNGG 4 cut(s) 134, 259, 315, 532
MvnI CGCG 2 cut(s) 376, 459
MwoI GCNNNNNNNGC 3 cut(s) 454, 547, 556
NarI GGCGCC 1 cut(s) 467
NciI CCSGG 4 cut(s) 134, 259, 315, 532
NcoI CCATGG 1 cut(s) 183
NdeII GATC 3 cut(s) 61, 165, 317
NlaIII CATG 3 cut(s) 187, 430, 611
NlaIV GGNNCC 6 cut(s) 224, 330, 343, 468, 529, 575
NmuCI GTSAC 2 cut(s) 309, 601
NspV TTCGAA 1 cut(s) 240
PauI GCGCGC 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 2 cut(s) 89, 266
PflMI CCANNNNNTGG 1 cut(s) 338
PfoI TCCNGGA 1 cut(s) 530
PinAI ACCGGT 1 cut(s) 306
PkrI GCNGC 4 cut(s) 27, 447, 491, 561
PleI GAGTC 2 cut(s) 459, 598
PluTI GGCGCC 1 cut(s) 470
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 2 cut(s) 459, 598
Ppu21I YACGTR 1 cut(s) 623
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
PspCI CACGTG 1 cut(s) 623
PspEI GGTNACC 1 cut(s) 309
PspN4I GGNNCC 6 cut(s) 224, 330, 343, 468, 529, 575
PspPI GGNCC 4 cut(s) 222, 261, 342, 528
PspPPI RGGWCCY 1 cut(s) 342
PteI GCGCGC 1 cut(s) 457
RsaI GTAC 2 cut(s) 74, 410
RsaNI GTAC 2 cut(s) 73, 409
RseI CAYNNNNRTG 2 cut(s) 182, 612
Rsr2I CGGWCCG 1 cut(s) 261
RsrII CGGWCCG 1 cut(s) 261
SalI GTCGAC 1 cut(s) 158
SatI GCNGC 4 cut(s) 26, 446, 490, 560
Sau3AI GATC 3 cut(s) 61, 165, 317
Sau96I GGNCC 4 cut(s) 222, 261, 342, 528
SchI GAGTC 2 cut(s) 460, 598
ScrFI CCNGG 4 cut(s) 134, 259, 315, 532
SduI GDGCHC 2 cut(s) 234, 543
SetI ASST 9 cut(s) 113, 144, 192, 337, 347, 390, 569, 603, 625
SfaNI GCATC 1 cut(s) 34
SfoI GGCGCC 1 cut(s) 468
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 4 cut(s) 222, 261, 342, 528
SmiMI CAYNNNNRTG 2 cut(s) 182, 612
Sse9I AATT 2 cut(s) 149, 370
SsiI CCGC 7 cut(s) 68, 114, 129, 201, 376, 445, 560
SspDI GGCGCC 1 cut(s) 466
StyD4I CCNGG 4 cut(s) 132, 257, 313, 530
StyI CCWWGG 1 cut(s) 183
TaiI ACGT 2 cut(s) 192, 625
TaqI TCGA 3 cut(s) 159, 240, 365
TasI AATT 2 cut(s) 149, 370
TauI GCSGC 2 cut(s) 448, 562
TfiI GAWTC 2 cut(s) 89, 266
TscAI CASTG 1 cut(s) 548
TseFI GTSAC 2 cut(s) 309, 601
TseI GCWGC 2 cut(s) 25, 489
Tsp45I GTSAC 2 cut(s) 309, 601
TspDTI ATGAA 4 cut(s) 54, 270, 382, 443
TspRI CASTG 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 4 cut(s) 222, 261, 342, 528
XapI RAATTY 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 159
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.