MD07G1177700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016924
Physical Location & Seq
Reverse (-)
25719911 .. 25720525
615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1177700.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGAAAATTTCTCAGATTTCAACCGGCAAGAAGTCGAAGAAGACGCACTGTCCTTCTGCGATCTCCCGCTACACGACGACGTTGGGGAGTCGGAGGAGAACCCTATGGCCTCCGCTTCTTATTCTTCCGACCTAGATGCCTTTTTTGAATTCCCGGTAGACCCGATCCGCGGATACATCCCCATGGACGTCGTTTTCTGTGGAAAACCAATCCACCTTCGAAACGAGGAGCTTCAAATCGAAAACCCATTTTTCTCCCGATCCGAATCATTCAAATTTTCATCTCCCTCCCACCCTCACTCGCACCGGTCGGAATCGGAGCTTGTGAGCTGCCGGTCGCACTCGCCGTCGAGCAATTCACGGAAGCACAAGGTGCTGATAGGACTGGTGAAGTACCAACCGGAGATGGACATGAATGAGATCAGAAAGCGGCAGGGTCGGCGCACTCCGGCGCCGATGTTCCCTGTGATAAGCGGCGGGGAGCTAGCTAGTGTAGGCGGTGGAAGGAGTGGGTCCGGTAAAGGGCATTGGCGGCTGATGAGGGCGCTGAGGTGTCGGTTCCACTTGTTAAACGCGTTAGCTAAGGCGACTCTTGGTTGTATGCCACGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.98

Weight (kDa)

6.5

Isoelectric Point (pI)

66.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016924)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20010
malus_domestica MD01G1111500.v1.1 MD07G1177700.v1.1
prunus_persica Prupe.2G217100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363961
rosa_laevigata RLG00000027532
rosa_multiflora Rmu_ssc0000167.1_g000035
rosa_roxburghii Rroxscaffold_4G00292800
rosa_rugosa Rorug01G0311700
rosa_samantha Rh1AG319500 Rh1BG282600 Rh1CG299000 Rh1DG314000
rosa_wichuraiana Rw1G028320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 159
AccII CGCG 2 cut(s) 171, 575
AciI CCGC 9 cut(s) 68, 114, 169, 171, 430, 474, 477, 498, 532
AclWI GGATC 2 cut(s) 160, 255
AcsI RAATTY 3 cut(s) 7, 149, 275
AcvI CACGTG 1 cut(s) 608
AcyI GRCGYC 2 cut(s) 189, 452
AdeI CACNNNGTG 1 cut(s) 373
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 2 cut(s) 170, 522
AflIII ACRYGT 2 cut(s) 573, 607
AgeI ACCGGT 1 cut(s) 306
AgsI TTSAA 4 cut(s) 22, 149, 236, 274
AhdI GACNNNNNGTC 1 cut(s) 49
AluBI AGCT 6 cut(s) 232, 322, 330, 484, 488, 581
AluI AGCT 6 cut(s) 232, 322, 330, 484, 488, 581
AlwI GGATC 2 cut(s) 160, 255
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 330
ApoI RAATTY 3 cut(s) 7, 149, 275
AsiGI ACCGGT 1 cut(s) 306
AspLEI GCGC 3 cut(s) 444, 454, 547
AspS9I GGNCC 1 cut(s) 513
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 400
AsuII TTCGAA 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 484
AvaII GGWCC 1 cut(s) 513
BanI GGYRCC 1 cut(s) 451
BbrPI CACGTG 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 48
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 1 cut(s) 317
BccI CCATC 1 cut(s) 400
BceAI ACGGC 1 cut(s) 331
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 155
BfaI CTAG 3 cut(s) 134, 485, 489
BfoI RGCGCY 2 cut(s) 455, 548
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 4 cut(s) 331, 431, 475, 533
BlsI GCNGC 4 cut(s) 332, 432, 476, 534
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 1 cut(s) 513
BmeRI GACNNNNNGTC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 513
BmiI GGNNCC 3 cut(s) 453, 514, 560
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 1 cut(s) 127
BmtI GCTAGC 1 cut(s) 488
BpiI GAAGAC 1 cut(s) 48
Bpu10I CCTNAGC 2 cut(s) 548, 582
Bpu14I TTCGAA 1 cut(s) 220
BpuMI CCSGG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 608
BsaBI GATNNNNATC 1 cut(s) 265
BsaHI GRCGYC 2 cut(s) 189, 452
BsaJI CCNNGG 2 cut(s) 169, 183
BsaWI WCCGGW 3 cut(s) 306, 400, 515
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 522
Bse118I RCCGGY 3 cut(s) 24, 306, 333
Bse1I ACTGG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 169, 183
BseGI GGATG 1 cut(s) 177
BseJI GATNNNNATC 1 cut(s) 265
BseLI CCNNNNNNNGG 2 cut(s) 170, 522
BseMII CTCAG 2 cut(s) 27, 539
BseNI ACTGG 1 cut(s) 390
BseRI GAGGAG 2 cut(s) 110, 242
BseXI GCAGC 1 cut(s) 317
Bsh1236I CGCG 2 cut(s) 171, 575
Bsh1285I CGRYCG 2 cut(s) 311, 338
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 1 cut(s) 451
BshTI ACCGGT 1 cut(s) 306
BsiEI CGRYCG 2 cut(s) 311, 338
BsiSI CCGG 7 cut(s) 25, 155, 307, 334, 401, 449, 516
BslI CCNNNNNNNGG 2 cut(s) 170, 522
BsnI GGCC 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 220
Bsp143I GATC 4 cut(s) 61, 165, 260, 420
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 9 cut(s) 68, 114, 169, 171, 430, 474, 477, 498, 532
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 26, 540
BspFNI CGCG 2 cut(s) 171, 575
BspLI GGNNCC 3 cut(s) 453, 514, 560
BspOI GCTAGC 1 cut(s) 488
BspPI GGATC 2 cut(s) 160, 255
BspT104I TTCGAA 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 451
BsrFI RCCGGY 3 cut(s) 24, 306, 333
BsrI ACTGG 1 cut(s) 390
BssAI RCCGGY 3 cut(s) 24, 306, 333
BssECI CCNNGG 2 cut(s) 169, 183
BssMI GATC 4 cut(s) 61, 165, 260, 420
BssNI GRCGYC 2 cut(s) 189, 452
BssT1I CCWWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 51
BstACI GRCGYC 2 cut(s) 189, 452
BstAPI GCANNNNNTGC 1 cut(s) 373
BstBAI YACGTR 1 cut(s) 608
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 3 cut(s) 13, 548, 582
BstDSI CCRYGG 2 cut(s) 169, 183
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 2 cut(s) 171, 575
BstH2I RGCGCY 2 cut(s) 455, 548
BstHHI GCGC 3 cut(s) 444, 454, 547
BstKTI GATC 4 cut(s) 64, 168, 263, 423
BstMBI GATC 4 cut(s) 61, 165, 260, 420
BstMCI CGRYCG 2 cut(s) 311, 338
BstMWI GCNNNNNNNGC 3 cut(s) 373, 439, 532
BstSCI CCNGG 1 cut(s) 153
BstUI CGCG 2 cut(s) 171, 575
BstV1I GCAGC 1 cut(s) 317
BstV2I GAAGAC 1 cut(s) 48
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 2 cut(s) 169, 183
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 486
CfoI GCGC 3 cut(s) 444, 454, 547
Cfr10I RCCGGY 3 cut(s) 24, 306, 333
Cfr13I GGNCC 1 cut(s) 513
Cfr42I CCGCGG 1 cut(s) 172
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 394
CspAI ACCGGT 1 cut(s) 306
CviAII CATG 2 cut(s) 184, 412
CviJI RGCY 8 cut(s) 110, 232, 322, 330, 484, 488, 535, 581
CviKI_1 RGCY 8 cut(s) 110, 232, 322, 330, 484, 488, 535, 581
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 3 cut(s) 13, 548, 582
DinI GGCGCC 1 cut(s) 453
DpnI GATC 4 cut(s) 63, 167, 262, 422
DpnII GATC 4 cut(s) 61, 165, 260, 420
DraIII CACNNNGTG 1 cut(s) 373
DriI GACNNNNNGTC 1 cut(s) 49
Eam1105I GACNNNNNGTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 513
Eco72I CACGTG 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 183
EgeI GGCGCC 1 cut(s) 453
EheI GGCGCC 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 2 cut(s) 187, 415
FaiI YATR 4 cut(s) 107, 185, 413, 602
FatI CATG 2 cut(s) 183, 411
FauI CCCGC 2 cut(s) 75, 470
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 331, 431, 475, 533
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 4 cut(s) 331, 431, 475, 533
FspBI CTAG 3 cut(s) 134, 485, 489
GlaI GCGC 3 cut(s) 443, 453, 546
GluI GCNGC 4 cut(s) 331, 431, 475, 533
HaeII RGCGCY 2 cut(s) 455, 548
HaeIII GGCC 1 cut(s) 110
HapII CCGG 7 cut(s) 25, 155, 307, 334, 401, 449, 516
HgaI GACGC 1 cut(s) 53
HhaI GCGC 3 cut(s) 444, 454, 547
Hin1I GRCGYC 2 cut(s) 189, 452
Hin1II CATG 2 cut(s) 187, 415
Hin6I GCGC 3 cut(s) 442, 452, 545
HinP1I GCGC 3 cut(s) 442, 452, 545
HinfI GANTC 4 cut(s) 89, 266, 314, 589
HpaII CCGG 7 cut(s) 25, 155, 307, 334, 401, 449, 516
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 8 cut(s) 16, 94, 130, 265, 313, 319, 425, 614
Hpy188III TCNNGA 1 cut(s) 258
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 4 cut(s) 80, 83, 194, 352
HpyAV CCTTC 3 cut(s) 64, 227, 498
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 3 cut(s) 81, 189, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 373, 439, 532
HpyF3I CTNAG 3 cut(s) 13, 548, 582
HpySE526I ACGT 3 cut(s) 81, 189, 607
Hsp92I GRCGYC 2 cut(s) 189, 452
Hsp92II CATG 2 cut(s) 187, 415
HspAI GCGC 3 cut(s) 442, 452, 545
KasI GGCGCC 1 cut(s) 451
KspI CCGCGG 1 cut(s) 172
Kzo9I GATC 4 cut(s) 61, 165, 260, 420
LmnI GCTCC 3 cut(s) 229, 319, 481
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 1 cut(s) 127
MaeI CTAG 3 cut(s) 134, 485, 489
MaeII ACGT 3 cut(s) 81, 189, 607
MalI GATC 4 cut(s) 63, 167, 262, 422
MboI GATC 4 cut(s) 61, 165, 260, 420
MboII GAAGA 3 cut(s) 50, 53, 117
MluCI AATT 4 cut(s) 7, 149, 275, 355
MluI ACGCGT 1 cut(s) 573
Mly113I GGCGCC 1 cut(s) 452
MlyI GAGTC 2 cut(s) 98, 583
MmeI TCCRAC 3 cut(s) 72, 153, 291
MnlI CCTC 7 cut(s) 88, 121, 220, 298, 306, 534, 543
MseI TTAA 1 cut(s) 569
MslI CAYNNNNRTG 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 7 cut(s) 25, 155, 307, 334, 401, 449, 516
MspR9I CCNGG 1 cut(s) 155
MvnI CGCG 2 cut(s) 171, 575
MwoI GCNNNNNNNGC 3 cut(s) 373, 439, 532
NarI GGCGCC 1 cut(s) 452
NciI CCSGG 1 cut(s) 155
NcoI CCATGG 1 cut(s) 183
NdeII GATC 4 cut(s) 61, 165, 260, 420
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 2 cut(s) 187, 415
NlaIV GGNNCC 3 cut(s) 453, 514, 560
NspV TTCGAA 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PfeI GAWTC 2 cut(s) 266, 314
PinAI ACCGGT 1 cut(s) 306
PkrI GCNGC 4 cut(s) 332, 432, 476, 534
PleI GAGTC 2 cut(s) 97, 583
PluTI GGCGCC 1 cut(s) 455
PmaCI CACGTG 1 cut(s) 608
PmlI CACGTG 1 cut(s) 608
PpsI GAGTC 2 cut(s) 97, 583
Ppu21I YACGTR 1 cut(s) 608
PspCI CACGTG 1 cut(s) 608
PspN4I GGNNCC 3 cut(s) 453, 514, 560
PspPI GGNCC 1 cut(s) 513
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 182
SacII CCGCGG 1 cut(s) 172
SaqAI TTAA 1 cut(s) 569
SatI GCNGC 4 cut(s) 331, 431, 475, 533
Sau3AI GATC 4 cut(s) 61, 165, 260, 420
Sau96I GGNCC 1 cut(s) 513
SchI GAGTC 2 cut(s) 98, 583
ScrFI CCNGG 1 cut(s) 155
SfaNI GCATC 1 cut(s) 127
SfoI GGCGCC 1 cut(s) 453
Sfr303I CCGCGG 1 cut(s) 172
SfuI TTCGAA 1 cut(s) 220
SgrBI CCGCGG 1 cut(s) 172
SinI GGWCC 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 182
Sse9I AATT 4 cut(s) 7, 149, 275, 355
SsiI CCGC 9 cut(s) 68, 114, 169, 171, 430, 474, 477, 498, 532
SspDI GGCGCC 1 cut(s) 451
SspMI CTAG 3 cut(s) 134, 485, 489
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 3 cut(s) 84, 192, 610
TaqI TCGA 4 cut(s) 36, 220, 240, 350
TasI AATT 4 cut(s) 7, 149, 275, 355
TauI GCSGC 3 cut(s) 433, 477, 535
TfiI GAWTC 2 cut(s) 266, 314
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 1 cut(s) 54
TseI GCWGC 1 cut(s) 330
TspDTI ATGAA 2 cut(s) 270, 428
TspGWI ACGGA 1 cut(s) 376
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 513
XapI RAATTY 3 cut(s) 7, 149, 275
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 3 cut(s) 134, 485, 489
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.