RLG00000027532

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016924
Physical Location & Seq
Forward (+)
12455574 .. 12461055
5482 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027532

Sequence Viewer

Length: 903 bp
ATGGAGTCTCACAACCCTCATTTTGAAGCTCCGGTACAAGACTTTGGTTTTGCATTTGAACAAGACCATGAAGAAGACGCACTTTCTCTTTGTGATCTCCCACTCCATGAACACAGCGAAGAAGAAAGCCCGGCCTCAGTTTCCGAGCCGGTAGACTTCTTTGAATTCGCTGTCGACCCTGTCTATGGCTACTACCCAATGGACATAGTCTTCTGCGGAAAATCCATCCCTTGCTCGAAGCCAATGAACAAACAATTTGGCTCCAGATCCGAGTCCTTCAAACTAGGACATTGTCCCAGTTCTCAATCTCACGTGATTCAACAACAGAGGAGCCTGAAATCGAAGTCTAGCAACTCACATAAGCACTTGATAGGGCTGGTGAAGTACCACCAGCAGGAGATGGACCTAAACGAGATAAGAAAGCGACAAGGCAGGCGAGCTCCGGTGGCAATGTTTCCGCTGAACGGCGGGGAGAAACCGGAAGTGAAGAACGAAGGGAAAGGGCACTCGGGTGTGATGAGACCGCTGAGGTGTCGGTCACACTTACTAAGCGCGTTGGCTAAGGCAGCTCTAGCAGGAGATGGACCTAAACGAGATAAGAAAGCGACAAGGCAGGCGAGCTCCGGTGGCAATGTTTCCGCTGAACGGCGGGGAGAAACCGGTGGCAATGCCTCCTTCGAGTTAGTTTTCCGCAAGTTCACTGTTCATGCATGCCTTGGAACCTTGACCTTGCCTGTTCAAATTCAGAAGAATTTCATTCAGTCTACCATGGAGACGGAGTTTATAGAGGCCTTGCGGGAGCTTAAGGACCATTTCAAGATCTTTGAGCAGATTAAAGGGTTCCTCCCCAGAAAGCTCCAATCTCTCTCCTTAGTCGACCCTGACCCAAATCTTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

33.63

Weight (kDa)

6.71

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016924)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20010
malus_domestica MD01G1111500.v1.1 MD07G1177700.v1.1
prunus_persica Prupe.2G217100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363961
rosa_laevigata RLG00000027532
rosa_multiflora Rmu_ssc0000167.1_g000035
rosa_roxburghii Rroxscaffold_4G00292800
rosa_rugosa Rorug01G0311700
rosa_samantha Rh1AG319500 Rh1BG282600 Rh1CG299000 Rh1DG314000
rosa_wichuraiana Rw1G028320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 153, 174, 764, 876
AccII CGCG 1 cut(s) 554
AciI CCGC 8 cut(s) 216, 458, 468, 524, 639, 649, 691, 796
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 3 cut(s) 164, 741, 751
AcvI CACGTG 1 cut(s) 313
AfaI GTAC 2 cut(s) 36, 386
AfiI CCNNNNNNNGG 4 cut(s) 185, 394, 464, 645
AflII CTTAAG 1 cut(s) 803
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 8 cut(s) 26, 59, 164, 280, 320, 740, 817, 896
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 6 cut(s) 29, 440, 569, 621, 802, 856
AluI AGCT 6 cut(s) 29, 440, 569, 621, 802, 856
Alw21I GWGCWC 2 cut(s) 442, 623
Alw26I GTCTC 3 cut(s) 12, 514, 767
AlwI GGATC 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 2 cut(s) 132, 789
ApeKI GCWGC 1 cut(s) 566
ApoI RAATTY 3 cut(s) 164, 741, 751
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AsiGI ACCGGT 1 cut(s) 659
Asp700I GAANNNNTTC 1 cut(s) 752
AspLEI GCGC 1 cut(s) 554
AspS9I GGNCC 3 cut(s) 403, 584, 808
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 508
AvaII GGWCC 3 cut(s) 403, 584, 808
BaeGI GKGCMC 1 cut(s) 507
BanII GRGCYC 2 cut(s) 442, 623
BarI GAAGNNNNNNTAC 2 cut(s) 18, 50
BbrPI CACGTG 1 cut(s) 313
BbsI GAAGAC 2 cut(s) 81, 202
Bbv12I GWGCWC 2 cut(s) 442, 623
BbvCI CCTCAGC 1 cut(s) 527
BbvI GCAGC 1 cut(s) 578
BccI CCATC 3 cut(s) 233, 394, 575
BceAI ACGGC 2 cut(s) 481, 662
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 3 cut(s) 12, 514, 767
BfaI CTAG 3 cut(s) 284, 348, 572
BfrI CTTAAG 1 cut(s) 803
BglII AGATCT 1 cut(s) 819
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 3 cut(s) 403, 584, 808
BmeT110I CYCGRG 1 cut(s) 508
BmgT120I GGNCC 3 cut(s) 403, 584, 808
BmiI GGNNCC 4 cut(s) 262, 332, 721, 842
BmrFI CCNGG 1 cut(s) 131
BmrI ACTGGG 1 cut(s) 291
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 81, 202
BpmI CTGGAG 1 cut(s) 247
Bpu10I CCTNAGC 2 cut(s) 527, 561
BpuMI CCSGG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 2 cut(s) 715, 768
BsaWI WCCGGW 5 cut(s) 31, 442, 478, 623, 659
BsaXI ACNNNNNCTCC 2 cut(s) 764, 794
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 394, 464, 645
Bse118I RCCGGY 2 cut(s) 148, 659
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 3 cut(s) 456, 637, 673
BseDI CCNNGG 2 cut(s) 715, 768
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 4 cut(s) 185, 394, 464, 645
BseMI GCAATG 3 cut(s) 456, 637, 673
BseMII CTCAG 2 cut(s) 150, 518
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 1 cut(s) 578
Bsh1236I CGCG 1 cut(s) 554
BshFI GGCC 2 cut(s) 134, 791
BshTI ACCGGT 1 cut(s) 659
BsiHKAI GWGCWC 2 cut(s) 442, 623
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 7 cut(s) 32, 131, 149, 443, 479, 624, 660
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 185, 394, 464, 645
BsmAI GTCTC 3 cut(s) 12, 514, 767
BsmBI CGTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 2 cut(s) 134, 791
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 508
Bsp1286I GDGCHC 3 cut(s) 442, 507, 623
Bsp143I GATC 3 cut(s) 94, 266, 819
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 8 cut(s) 216, 458, 468, 524, 639, 649, 691, 796
BspANI GGCC 2 cut(s) 134, 791
BspCNI CTCAG 2 cut(s) 149, 519
BspFNI CGCG 1 cut(s) 554
BspLI GGNNCC 4 cut(s) 262, 332, 721, 842
BspPI GGATC 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 514
BsrDI GCAATG 3 cut(s) 456, 637, 673
BsrFI RCCGGY 2 cut(s) 148, 659
BsrI ACTGG 1 cut(s) 297
BssAI RCCGGY 2 cut(s) 148, 659
BssECI CCNNGG 2 cut(s) 715, 768
BssMI GATC 3 cut(s) 94, 266, 819
BssT1I CCWWGG 2 cut(s) 715, 768
Bst4CI ACNGT 1 cut(s) 703
BstAFI CTTAAG 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 313
BstC8I GCNNGC 5 cut(s) 434, 438, 615, 619, 712
BstDEI CTNAG 5 cut(s) 136, 527, 548, 561, 871
BstDSI CCRYGG 1 cut(s) 768
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 1 cut(s) 554
BstHHI GCGC 1 cut(s) 554
BstKTI GATC 3 cut(s) 97, 269, 822
BstMAI GTCTC 3 cut(s) 12, 514, 767
BstMBI GATC 3 cut(s) 94, 266, 819
BstMWI GCNNNNNNNGC 4 cut(s) 446, 566, 572, 627
BstNSI RCATGY 1 cut(s) 714
BstSCI CCNGG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 507
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 1 cut(s) 578
BstV2I GAAGAC 2 cut(s) 81, 202
BstX2I RGATCY 2 cut(s) 266, 819
BstYI RGATCY 2 cut(s) 266, 819
BsuRI GGCC 2 cut(s) 134, 791
BtgI CCRYGG 1 cut(s) 768
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 5 cut(s) 434, 438, 615, 619, 712
CfoI GCGC 1 cut(s) 554
Cfr10I RCCGGY 2 cut(s) 148, 659
Cfr13I GGNCC 3 cut(s) 403, 584, 808
CseI GACGC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 35, 385
CspAI ACCGGT 1 cut(s) 659
CviAII CATG 5 cut(s) 68, 107, 707, 711, 769
CviQI GTAC 2 cut(s) 35, 385
DdeI CTNAG 5 cut(s) 136, 527, 548, 561, 871
DpnI GATC 3 cut(s) 96, 268, 821
DpnII GATC 3 cut(s) 94, 266, 819
Ecl136II GAGCTC 2 cut(s) 440, 621
Eco130I CCWWGG 2 cut(s) 715, 768
Eco147I AGGCCT 1 cut(s) 791
Eco24I GRGCYC 2 cut(s) 442, 623
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 3 cut(s) 403, 584, 808
Eco53kI GAGCTC 2 cut(s) 440, 621
Eco72I CACGTG 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 508
EcoICRI GAGCTC 2 cut(s) 440, 621
EcoRI GAATTC 1 cut(s) 164
EcoT14I CCWWGG 2 cut(s) 715, 768
EcoT22I ATGCAT 1 cut(s) 712
EcoT38I GRGCYC 2 cut(s) 442, 623
ErhI CCWWGG 2 cut(s) 715, 768
Esp3I CGTCTC 1 cut(s) 767
FaeI CATG 5 cut(s) 71, 110, 710, 714, 772
FaiI YATR 9 cut(s) 69, 108, 186, 206, 360, 708, 712, 770, 785
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 1 cut(s) 279
FatI CATG 5 cut(s) 67, 106, 706, 710, 768
FauI CCCGC 3 cut(s) 461, 642, 789
FblI GTMKAC 4 cut(s) 153, 174, 764, 876
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 2 cut(s) 442, 623
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 3 cut(s) 284, 348, 572
GlaI GCGC 1 cut(s) 553
GluI GCNGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 2 cut(s) 134, 791
HapII CCGG 7 cut(s) 32, 131, 149, 443, 479, 624, 660
HgaI GACGC 1 cut(s) 86
HhaI GCGC 1 cut(s) 554
Hin1II CATG 5 cut(s) 71, 110, 710, 714, 772
Hin6I GCGC 1 cut(s) 552
HinP1I GCGC 1 cut(s) 552
HincII GTYRAC 2 cut(s) 175, 877
HindII GTYRAC 2 cut(s) 175, 877
HinfI GANTC 3 cut(s) 5, 272, 316
HpaII CCGG 7 cut(s) 32, 131, 149, 443, 479, 624, 660
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 5 cut(s) 154, 175, 699, 765, 877
Hpy188I TCNGA 3 cut(s) 145, 271, 747
Hpy188III TCNNGA 3 cut(s) 264, 817, 893
Hpy8I GTNNAC 5 cut(s) 154, 175, 699, 765, 877
HpyAV CCTTC 3 cut(s) 286, 488, 685
HpyCH4III ACNGT 1 cut(s) 703
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 53, 710
HpyF10VI GCNNNNNNNGC 4 cut(s) 446, 566, 572, 627
HpyF3I CTNAG 5 cut(s) 136, 527, 548, 561, 871
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 5 cut(s) 71, 110, 710, 714, 772
HspAI GCGC 1 cut(s) 552
Kzo9I GATC 3 cut(s) 94, 266, 819
LmnI GCTCC 7 cut(s) 34, 266, 330, 445, 626, 799, 861
Lsp1109I GCAGC 1 cut(s) 578
MaeI CTAG 3 cut(s) 284, 348, 572
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 537
MalI GATC 3 cut(s) 96, 268, 821
MboI GATC 3 cut(s) 94, 266, 819
MboII GAAGA 7 cut(s) 83, 86, 131, 134, 202, 499, 760
MflI RGATCY 2 cut(s) 266, 819
MhlI GDGCHC 3 cut(s) 442, 507, 623
MluCI AATT 4 cut(s) 164, 254, 741, 751
MlyI GAGTC 2 cut(s) 14, 281
MnlI CCTC 7 cut(s) 27, 145, 321, 522, 682, 781, 854
Mph1103I ATGCAT 1 cut(s) 712
MroXI GAANNNNTTC 1 cut(s) 752
MseI TTAA 2 cut(s) 804, 834
MslI CAYNNNNRTG 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 460, 526, 641
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 7 cut(s) 32, 131, 149, 443, 479, 624, 660
MspR9I CCNGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 4 cut(s) 446, 566, 572, 627
NciI CCSGG 1 cut(s) 131
NcoI CCATGG 1 cut(s) 768
NdeII GATC 3 cut(s) 94, 266, 819
NlaIII CATG 5 cut(s) 71, 110, 710, 714, 772
NlaIV GGNNCC 4 cut(s) 262, 332, 721, 842
NmuCI GTSAC 1 cut(s) 537
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 1 cut(s) 714
OliI CACNNNNGTG 1 cut(s) 510
PaeI GCATGC 1 cut(s) 714
PceI AGGCCT 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 1 cut(s) 316
PflFI GACNNNGTC 3 cut(s) 179, 206, 291
PinAI ACCGGT 1 cut(s) 659
PkrI GCNGC 1 cut(s) 568
PleI GAGTC 2 cut(s) 13, 280
PmaCI CACGTG 1 cut(s) 313
PmlI CACGTG 1 cut(s) 313
PpsI GAGTC 2 cut(s) 13, 280
Ppu21I YACGTR 1 cut(s) 313
Psp124BI GAGCTC 2 cut(s) 442, 623
PspCI CACGTG 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 262, 332, 721, 842
PspPI GGNCC 3 cut(s) 403, 584, 808
PsuI RGATCY 2 cut(s) 266, 819
PsyI GACNNNGTC 3 cut(s) 179, 206, 291
RsaI GTAC 2 cut(s) 36, 386
RsaNI GTAC 2 cut(s) 35, 385
RseI CAYNNNNRTG 1 cut(s) 510
SacI GAGCTC 2 cut(s) 442, 623
SalI GTCGAC 2 cut(s) 173, 875
SaqAI TTAA 2 cut(s) 804, 834
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 3 cut(s) 94, 266, 819
Sau96I GGNCC 3 cut(s) 403, 584, 808
SchI GAGTC 2 cut(s) 14, 281
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 3 cut(s) 442, 507, 623
SinI GGWCC 3 cut(s) 403, 584, 808
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
SphI GCATGC 1 cut(s) 714
Sse9I AATT 4 cut(s) 164, 254, 741, 751
SseBI AGGCCT 1 cut(s) 791
SsiI CCGC 8 cut(s) 216, 458, 468, 524, 639, 649, 691, 796
SspMI CTAG 3 cut(s) 284, 348, 572
SstI GAGCTC 2 cut(s) 442, 623
StuI AGGCCT 1 cut(s) 791
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 2 cut(s) 715, 768
TaaI ACNGT 1 cut(s) 703
TaiI ACGT 1 cut(s) 315
TaqI TCGA 5 cut(s) 174, 236, 341, 678, 876
TaqII GACCGA 1 cut(s) 525
TasI AATT 4 cut(s) 164, 254, 741, 751
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 2 cut(s) 804, 834
Tru9I TTAA 2 cut(s) 804, 834
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 1 cut(s) 537
TseI GCWGC 1 cut(s) 566
Tsp45I GTSAC 1 cut(s) 537
TspDTI ATGAA 5 cut(s) 84, 123, 260, 695, 745
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 1 cut(s) 706
Tth111I GACNNNGTC 3 cut(s) 179, 206, 291
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 3 cut(s) 403, 584, 808
XapI RAATTY 3 cut(s) 164, 741, 751
XceI RCATGY 1 cut(s) 714
XmiI GTMKAC 4 cut(s) 153, 174, 764, 876
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 3 cut(s) 284, 348, 572
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.