Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013166

Physical Location & Seq

Genomic Coordinates Forward (+)

24346584 .. 24349886

Gene Structure 3303 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1133600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGAAGGTGACCGTAGTTTCGCGGAGTGGCCGAGAAGTTGTCAAGGGCGGCCTTGAGCTCACCGATTCTGCCACCGTAGCTGATCTGCAGGATGCAATCTATAAGCGAACCAAAAAGTTTTATCCTGCAAGACAGCGGCTTACTCTCCCCGTCCAACCAGGATCAAAAGAAAGGCCTGTTGTCCTTAGCTACAAAAAGAGTCTCCAAGATTACATCAGTGGGAACTCAGATAACTTAACTGTGGTGTTCAAGGACCTGGGGCCACAAGTGTCCTACAGAACACTCTTCTTCTTTGAGTATTTGGGCCCTTTGATCCTTTATCCCATCTTTTACTACTTCCCTGTGTACGACTACTTGGGCTTCAAGGGAGACCGTGTCATCCACCCGGTCCAGACATATGCTCTATACTACTGGTGCTTCCACTACTTCAAACGTATTATGGAAACATTTTTCGTACATCGCTTCAGCCATGCAACCTCACCACTCTCCAATGTATTCCGGAATTGTGCTTACTATTGGAGCTTTGGCGCTTTCATAGCTTATTACCTGAATCACCCGCTTTACACCCCTGTTAGTGACCTCCAAATGAAGATCGGGTTTGGTTTTGGGATAATATGTCAAATCTCAAACTTGTATTGTCATATCCTGTTGAGGAACCTGCGAAGCCCTGATGGAAATGGAGGATATCAAATCCCACGGGGTTTTCTGTTCAATATTGTGACATGTGCAAATTACACAACAGAGATCTATCAATGGTTGGGCTTCAATATAGCAACGCAGACAGTCGCTGGTTATATCTTTCTCATAGTTGCTGCTTCCATCATGACCAACTGGGCGCTTACAAAGCACCGACGTCTTAAGAAGATATTTGATGGGAAAGACGGAAGACCCAAGTATCCGCGCCGATGGGTGATCCTACCTCCATTCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004312 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006643 GO:0006665 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009888 GO:0009922 GO:0009923 GO:0009987 GO:0010025 GO:0010026 GO:0010090 GO:0010091 GO:0010166 GO:0010927 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016053 GO:0016491 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030176 GO:0030497 GO:0031224 GO:0031227 GO:0031984 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0032991 GO:0042175 GO:0042335 GO:0042761 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0055114 GO:0071704 GO:0071840 GO:0071944 GO:0072330 GO:0090558 GO:0090626 GO:0098796 GO:0098827 GO:1901564 GO:1901568 GO:1901570 GO:1901576 GO:1902494 GO:1905499 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

311

Amino Acids

36.07

Weight (kDa)

9.63

Isoelectric Point (pI)

46.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	159 - 309	1.9e-28	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55360
fragaria_vesca	FvH4_7g22400
malus_domestica	MD01G1133600.v1.1 MD07G1197900.v1.1
prunus_persica	Prupe.2G234700_v2.0.a1
pyrus_communis	pycom01g15440 pycom07g18510
rosa_chinensis	RchiOBHm_Chr1g0367031
rosa_laevigata	RLG00000027305
rosa_multiflora	Rmu_sc0013700.1_g000003
rosa_roxburghii	Rroxscaffold_4G00289690
rosa_rugosa	Rorug01G0334100
rosa_samantha	Rh1AG340800 Rh1BG301300 Rh1CG317500 Rh1DG333700
rosa_wichuraiana	Rw0G023880 Rw1G030270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	856
Acc36I	ACCTGC	1 cut(s)	666
AccII	CGCG	2 cut(s)	22, 901
AccIII	TCCGGA	1 cut(s)	498
AciI	CCGC	5 cut(s)	22, 48, 136, 557, 899
AclWI	GGATC	3 cut(s)	169, 307, 907
AcoI	YGGCCR	1 cut(s)	28
AcuI	CTGAAG	1 cut(s)	448
AcyI	GRCGYC	1 cut(s)	853
AfaI	GTAC	2 cut(s)	347, 456
AflII	CTTAAG	1 cut(s)	857
AflIII	ACRYGT	1 cut(s)	722
AgsI	TTSAA	5 cut(s)	250, 364, 430, 712, 766
AjnI	CCWGG	2 cut(s)	157, 255
AluBI	AGCT	5 cut(s)	58, 80, 189, 522, 539
AluI	AGCT	5 cut(s)	58, 80, 189, 522, 539
Alw21I	GWGCWC	1 cut(s)	60
Alw26I	GTCTC	2 cut(s)	206, 363
AlwI	GGATC	3 cut(s)	169, 307, 907
AlwNI	CAGNNNCTG	1 cut(s)	788
Aor13HI	TCCGGA	1 cut(s)	498
AoxI	GGCC	5 cut(s)	28, 49, 173, 260, 304
ApaI	GGGCCC	1 cut(s)	308
ApeKI	GCWGC	1 cut(s)	812
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AspLEI	GCGC	3 cut(s)	530, 838, 903
AspS9I	GGNCC	5 cut(s)	253, 260, 304, 305, 388
AsuC2I	CCSGG	1 cut(s)	386
AsuHPI	GGTGA	5 cut(s)	19, 52, 471, 545, 922
AvaII	GGWCC	2 cut(s)	253, 388
BaeGI	GKGCMC	1 cut(s)	308
BaeI	ACNNNNGTAYC	2 cut(s)	878, 911
BanII	GRGCYC	2 cut(s)	60, 308
BbsI	GAAGAC	1 cut(s)	892
Bbv12I	GWGCWC	1 cut(s)	60
BbvI	GCAGC	1 cut(s)	799
BccI	CCATC	5 cut(s)	332, 665, 827, 866, 900
BciT130I	CCWGG	2 cut(s)	159, 257
BciVI	GTATCC	1 cut(s)	906
BcnI	CCSGG	1 cut(s)	386
BcoDI	GTCTC	2 cut(s)	206, 363
BfmI	CTRYAG	3 cut(s)	86, 274, 929
BfoI	RGCGCY	2 cut(s)	531, 839
BfrI	CTTAAG	1 cut(s)	857
BfuAI	ACCTGC	1 cut(s)	666
BfuI	GTATCC	1 cut(s)	906
BglII	AGATCT	1 cut(s)	744
BisI	GCNGC	3 cut(s)	49, 137, 813
BlsI	GCNGC	3 cut(s)	50, 138, 814
Bme1390I	CCNGG	3 cut(s)	159, 257, 386
Bme18I	GGWCC	2 cut(s)	253, 388
BmgT120I	GGNCC	5 cut(s)	253, 260, 304, 305, 388
BmiI	GGNNCC	3 cut(s)	261, 306, 656
BmrFI	CCNGG	3 cut(s)	159, 257, 386
BmrI	ACTGGG	1 cut(s)	841
BmsI	GCATC	1 cut(s)	82
BmuI	ACTGGG	1 cut(s)	841
BpiI	GAAGAC	1 cut(s)	892
Bpu10I	CCTNAGC	1 cut(s)	185
BpuEI	CTTGAG	1 cut(s)	74
BpuMI	CCSGG	1 cut(s)	386
BsaHI	GRCGYC	1 cut(s)	853
BsaI	GGTCTC	1 cut(s)	363
BsaJI	CCNNGG	2 cut(s)	256, 695
BsaWI	WCCGGW	1 cut(s)	498
BsaXI	ACNNNNNCTCC	2 cut(s)	360, 390
Bse1I	ACTGG	2 cut(s)	416, 836
BseAI	TCCGGA	1 cut(s)	498
BseBI	CCWGG	2 cut(s)	159, 257
BseDI	CCNNGG	2 cut(s)	256, 695
BseGI	GGATG	2 cut(s)	97, 378
BseMII	CTCAG	1 cut(s)	240
BseNI	ACTGG	2 cut(s)	416, 836
BseSI	GKGCMC	1 cut(s)	308
BseXI	GCAGC	1 cut(s)	799
Bsh1236I	CGCG	2 cut(s)	22, 901
BshFI	GGCC	5 cut(s)	30, 51, 175, 262, 306
BsiHKAI	GWGCWC	1 cut(s)	60
BsiSI	CCGG	2 cut(s)	386, 499
BsmAI	GTCTC	2 cut(s)	206, 363
BsnI	GGCC	5 cut(s)	30, 51, 175, 262, 306
Bso31I	GGTCTC	1 cut(s)	363
Bsp120I	GGGCCC	1 cut(s)	304
Bsp1286I	GDGCHC	2 cut(s)	60, 308
Bsp13I	TCCGGA	1 cut(s)	498
Bsp143I	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
BspACI	CCGC	5 cut(s)	22, 48, 136, 557, 899
BspANI	GGCC	5 cut(s)	30, 51, 175, 262, 306
BspCNI	CTCAG	1 cut(s)	239
BspEI	TCCGGA	1 cut(s)	498
BspFNI	CGCG	2 cut(s)	22, 901
BspHI	TCATGA	1 cut(s)	822
BspLI	GGNNCC	3 cut(s)	261, 306, 656
BspMAI	CTGCAG	1 cut(s)	90
BspMI	ACCTGC	1 cut(s)	666
BspPI	GGATC	3 cut(s)	169, 307, 907
BspTI	CTTAAG	1 cut(s)	857
BspTNI	GGTCTC	1 cut(s)	363
BsrI	ACTGG	2 cut(s)	416, 836
BssECI	CCNNGG	2 cut(s)	256, 695
BssMI	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
BssNI	GRCGYC	1 cut(s)	853
Bst2UI	CCWGG	2 cut(s)	159, 257
Bst4CI	ACNGT	5 cut(s)	13, 76, 241, 374, 784
Bst6I	CTCTTC	1 cut(s)	290
BstACI	GRCGYC	1 cut(s)	853
BstAFI	CTTAAG	1 cut(s)	857
BstDEI	CTNAG	2 cut(s)	185, 226
BstDSI	CCRYGG	1 cut(s)	695
BstEII	GGTNACC	1 cut(s)	7
BstF5I	GGATG	2 cut(s)	97, 378
BstFNI	CGCG	2 cut(s)	22, 901
BstH2I	RGCGCY	2 cut(s)	531, 839
BstHHI	GCGC	3 cut(s)	530, 838, 903
BstKTI	GATC	6 cut(s)	85, 164, 315, 594, 747, 915
BstMAI	GTCTC	2 cut(s)	206, 363
BstMBI	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
BstMWI	GCNNNNNNNGC	3 cut(s)	77, 536, 844
BstNI	CCWGG	2 cut(s)	159, 257
BstNSI	RCATGY	1 cut(s)	726
BstPI	GGTNACC	1 cut(s)	7
BstSCI	CCNGG	3 cut(s)	157, 255, 384
BstSFI	CTRYAG	3 cut(s)	86, 274, 929
BstSLI	GKGCMC	1 cut(s)	308
BstUI	CGCG	2 cut(s)	22, 901
BstV1I	GCAGC	1 cut(s)	799
BstV2I	GAAGAC	1 cut(s)	892
BstX2I	RGATCY	1 cut(s)	744
BstYI	RGATCY	1 cut(s)	744
BsuI	GTATCC	1 cut(s)	906
BsuRI	GGCC	5 cut(s)	30, 51, 175, 262, 306
BtgI	CCRYGG	1 cut(s)	695
BtgZI	GCGATG	1 cut(s)	443
BtsCI	GGATG	2 cut(s)	97, 378
BtsIMutI	CAGTG	1 cut(s)	223
BveI	ACCTGC	1 cut(s)	666
CaiI	CAGNNNCTG	1 cut(s)	788
CciI	TCATGA	1 cut(s)	822
CfoI	GCGC	3 cut(s)	530, 838, 903
Cfr13I	GGNCC	5 cut(s)	253, 260, 304, 305, 388
Csp6I	GTAC	2 cut(s)	346, 455
CviAII	CATG	3 cut(s)	470, 723, 823
CviQI	GTAC	2 cut(s)	346, 455
DdeI	CTNAG	2 cut(s)	185, 226
DpnI	GATC	6 cut(s)	84, 163, 314, 593, 746, 914
DpnII	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
EaeI	YGGCCR	1 cut(s)	28
Eam1104I	CTCTTC	1 cut(s)	290
EarI	CTCTTC	1 cut(s)	290
Ecl136II	GAGCTC	1 cut(s)	58
Eco147I	AGGCCT	1 cut(s)	175
Eco24I	GRGCYC	2 cut(s)	60, 308
Eco31I	GGTCTC	1 cut(s)	363
Eco32I	GATATC	1 cut(s)	686
Eco47I	GGWCC	2 cut(s)	253, 388
Eco53kI	GAGCTC	1 cut(s)	58
Eco57I	CTGAAG	1 cut(s)	448
Eco91I	GGTNACC	1 cut(s)	7
EcoICRI	GAGCTC	1 cut(s)	58
EcoO109I	RGGNCCY	2 cut(s)	253, 305
EcoO65I	GGTNACC	1 cut(s)	7
EcoRII	CCWGG	2 cut(s)	157, 255
EcoRV	GATATC	1 cut(s)	686
EcoT38I	GRGCYC	2 cut(s)	60, 308
FaeI	CATG	3 cut(s)	473, 726, 826
FatI	CATG	3 cut(s)	469, 722, 822
FauI	CCCGC	1 cut(s)	564
FauNDI	CATATG	1 cut(s)	397
Fnu4HI	GCNGC	3 cut(s)	49, 137, 813
FokI	GGATG	2 cut(s)	104, 365
FriOI	GRGCYC	2 cut(s)	60, 308
Fsp4HI	GCNGC	3 cut(s)	49, 137, 813
GlaI	GCGC	3 cut(s)	529, 837, 902
GluI	GCNGC	3 cut(s)	49, 137, 813
HaeII	RGCGCY	2 cut(s)	531, 839
HaeIII	GGCC	5 cut(s)	30, 51, 175, 262, 306
HapII	CCGG	2 cut(s)	386, 499
HhaI	GCGC	3 cut(s)	530, 838, 903
Hin1I	GRCGYC	1 cut(s)	853
Hin1II	CATG	3 cut(s)	473, 726, 826
Hin6I	GCGC	3 cut(s)	528, 836, 901
HinP1I	GCGC	3 cut(s)	528, 836, 901
HinfI	GANTC	3 cut(s)	65, 199, 550
HpaII	CCGG	2 cut(s)	386, 499
HphI	GGTGA	5 cut(s)	19, 52, 471, 545, 922
Hpy166II	GTNNAC	1 cut(s)	346
Hpy188I	TCNGA	1 cut(s)	229
Hpy188III	TCNNGA	3 cut(s)	391, 499, 823
Hpy8I	GTNNAC	1 cut(s)	346
Hpy99I	CGWCG	1 cut(s)	855
HpyCH4III	ACNGT	5 cut(s)	13, 76, 241, 374, 784
HpyCH4IV	ACGT	2 cut(s)	433, 853
HpyCH4V	TGCA	5 cut(s)	88, 95, 128, 473, 728
HpyF10VI	GCNNNNNNNGC	3 cut(s)	77, 536, 844
HpyF3I	CTNAG	2 cut(s)	185, 226
HpySE526I	ACGT	2 cut(s)	433, 853
Hsp92I	GRCGYC	1 cut(s)	853
Hsp92II	CATG	3 cut(s)	473, 726, 826
HspAI	GCGC	3 cut(s)	528, 836, 901
Kpn2I	TCCGGA	1 cut(s)	498
Kzo9I	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
LmnI	GCTCC	1 cut(s)	519
Lsp1109I	GCAGC	1 cut(s)	799
LweI	GCATC	1 cut(s)	82
MaeII	ACGT	2 cut(s)	433, 853
MaeIII	GTNAC	3 cut(s)	7, 575, 718
MalI	GATC	6 cut(s)	84, 163, 314, 593, 746, 914
MboI	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
MboII	GAAGA	5 cut(s)	277, 280, 601, 874, 897
MflI	RGATCY	1 cut(s)	744
MhlI	GDGCHC	2 cut(s)	60, 308
MluCI	AATT	2 cut(s)	502, 730
MlyI	GAGTC	1 cut(s)	208
MmeI	TCCRAC	1 cut(s)	178
MnlI	CCTC	5 cut(s)	487, 590, 645, 674, 930
MroI	TCCGGA	1 cut(s)	498
MseI	TTAA	2 cut(s)	236, 858
MspA1I	CMGCKG	1 cut(s)	136
MspCI	CTTAAG	1 cut(s)	857
MspI	CCGG	2 cut(s)	386, 499
MspR9I	CCNGG	3 cut(s)	159, 257, 386
MvaI	CCWGG	2 cut(s)	159, 257
MvnI	CGCG	2 cut(s)	22, 901
MwoI	GCNNNNNNNGC	3 cut(s)	77, 536, 844
NciI	CCSGG	1 cut(s)	386
NdeI	CATATG	1 cut(s)	397
NdeII	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
NlaIII	CATG	3 cut(s)	473, 726, 826
NlaIV	GGNNCC	3 cut(s)	261, 306, 656
NmeAIII	GCCGAG	1 cut(s)	56
NmuCI	GTSAC	3 cut(s)	7, 575, 718
NspI	RCATGY	1 cut(s)	726
PagI	TCATGA	1 cut(s)	822
PceI	AGGCCT	1 cut(s)	175
PciI	ACATGT	1 cut(s)	722
PfeI	GAWTC	2 cut(s)	65, 550
PflFI	GACNNNGTC	1 cut(s)	374
PkrI	GCNGC	3 cut(s)	50, 138, 814
PleI	GAGTC	1 cut(s)	207
PpsI	GAGTC	1 cut(s)	207
PpuMI	RGGWCCY	1 cut(s)	253
PscI	ACATGT	1 cut(s)	722
Psp124BI	GAGCTC	1 cut(s)	60
Psp5II	RGGWCCY	1 cut(s)	253
Psp6I	CCWGG	2 cut(s)	157, 255
PspEI	GGTNACC	1 cut(s)	7
PspGI	CCWGG	2 cut(s)	157, 255
PspN4I	GGNNCC	3 cut(s)	261, 306, 656
PspOMI	GGGCCC	1 cut(s)	304
PspPI	GGNCC	5 cut(s)	253, 260, 304, 305, 388
PspPPI	RGGWCCY	1 cut(s)	253
PstI	CTGCAG	1 cut(s)	90
PstNI	CAGNNNCTG	1 cut(s)	788
PsuI	RGATCY	1 cut(s)	744
PsyI	GACNNNGTC	1 cut(s)	374
RsaI	GTAC	2 cut(s)	347, 456
RsaNI	GTAC	2 cut(s)	346, 455
SacI	GAGCTC	1 cut(s)	60
SaqAI	TTAA	2 cut(s)	236, 858
SatI	GCNGC	3 cut(s)	49, 137, 813
Sau3AI	GATC	6 cut(s)	82, 161, 312, 591, 744, 912
Sau96I	GGNCC	5 cut(s)	253, 260, 304, 305, 388
SchI	GAGTC	1 cut(s)	208
ScrFI	CCNGG	3 cut(s)	159, 257, 386
SduI	GDGCHC	2 cut(s)	60, 308
SfaNI	GCATC	1 cut(s)	82
SfcI	CTRYAG	3 cut(s)	86, 274, 929
SinI	GGWCC	2 cut(s)	253, 388
SmlI	CTYRAG	2 cut(s)	53, 857
SmoI	CTYRAG	2 cut(s)	53, 857
Sse9I	AATT	2 cut(s)	502, 730
SseBI	AGGCCT	1 cut(s)	175
SsiI	CCGC	5 cut(s)	22, 48, 136, 557, 899
SspI	AATATT	1 cut(s)	715
SstI	GAGCTC	1 cut(s)	60
StuI	AGGCCT	1 cut(s)	175
StyD4I	CCNGG	3 cut(s)	157, 255, 384
TaaI	ACNGT	5 cut(s)	13, 76, 241, 374, 784
TaiI	ACGT	2 cut(s)	436, 856
TasI	AATT	2 cut(s)	502, 730
TauI	GCSGC	2 cut(s)	51, 139
TfiI	GAWTC	2 cut(s)	65, 550
Tru1I	TTAA	2 cut(s)	236, 858
Tru9I	TTAA	2 cut(s)	236, 858
TscAI	CASTG	1 cut(s)	223
TseFI	GTSAC	3 cut(s)	7, 575, 718
TseI	GCWGC	1 cut(s)	812
Tsp45I	GTSAC	3 cut(s)	7, 575, 718
TspDTI	ATGAA	3 cut(s)	17, 523, 602
TspGWI	ACGGA	1 cut(s)	897
TspRI	CASTG	1 cut(s)	223
Tth111I	GACNNNGTC	1 cut(s)	374
Vha464I	CTTAAG	1 cut(s)	857
VpaK11BI	GGWCC	2 cut(s)	253, 388
XceI	RCATGY	1 cut(s)	726
ZraI	GACGTC	1 cut(s)	854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.