Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013166

Physical Location & Seq

Genomic Coordinates Forward (+)

27637280 .. 27641708

Gene Structure 4429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1197900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGAAGGTGACCGTAGTTTCGCGGAGTGGCCGAGAAGTTGTCAAGGGCGGCCTTGAGCTCAGCGATTCTGCCACAGTAGCTGATCTGCAGGATGCAATCCATAAGCGAACCAAAAAGTTCTATCCTGCAAGACAGCGGCTTACTCTCCCCGTCCAACCAGGATCAAAAGAGAGGCCGGTTGTCCTTAGCTACAAGAAGAGTCTCCAAGATTACATCAGCGGAAACTCAGACAACCTAACTGTGGTGTTCAAGGACCTAGGTCCACAAGTGTCATACAGAACACTCTTCTTCTTTGAGTATTTGGGCCCTTTGATCCTTTACCCCATCTTTTACTACTTCCCTGTTTACGATTATTTGGGCTTCAAAGGAGACCGTGTCATCCACCCTGTCCAGACATATGCCCTATACTACTGGTGCTTCCACTACTTCAAACGTATTATGGAAACATTTTTTGGCCGAAGGGGTAGAGGAAGACCTAGGAAAACTTTGGAAGAGACTCTAAGAAAAGACTTAGAGTACTTGGATCGAACGGAAGACATGACACAAAACCGAGCGCAATGGCGTTCTAGGATTCATATAGCCGACCCCACTTACCATGCAACCTCACCACTCTCCAATGTATTCCGGAATTGTGCTTATTATTGGAGCTTTGGTGCTTTCATAGCTTACTACCTGAATCACCCGCTTTACACCCCTGTTAGTGACCTCCAAATGAAGATTGGGTTTGGTATTGGGATAATATGTCAAATCTCAAACTTCTATTGCCATATCCTTTTGAGGAACCTGCGAAGCCCTGATGGGAATGGAGGATATCAGATCCCACGTGGTTTTCTGTTCAATATTGTGACATGTGCAAATTACACTACAGAGATCTATCAGTGGTTGGGCTTCAATATAGCAACACAGACAGTTGCTGGCTATATCTTTCTCATAGTTGCTGCTTCTATCATGACCAATTGGGCCCTTGCCAAGCACCGCCGTCTTAAGAAGATATTTGACGGCAAAGACGGAAGACCCAAGTATCCACGCCGATGGGTGATCCTACCTCCATTCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004312 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006643 GO:0006665 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009888 GO:0009922 GO:0009923 GO:0009987 GO:0010025 GO:0010026 GO:0010090 GO:0010091 GO:0010166 GO:0010927 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016053 GO:0016491 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030176 GO:0030497 GO:0031224 GO:0031227 GO:0031984 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0032991 GO:0042175 GO:0042335 GO:0042761 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0055114 GO:0071704 GO:0071840 GO:0071944 GO:0072330 GO:0090558 GO:0090626 GO:0098796 GO:0098827 GO:1901564 GO:1901568 GO:1901570 GO:1901576 GO:1902494 GO:1905499 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

353

Amino Acids

41.1

Weight (kDa)

9.71

Isoelectric Point (pI)

50.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	201 - 351	5.2e-29	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55360
fragaria_vesca	FvH4_7g22400
malus_domestica	MD01G1133600.v1.1 MD07G1197900.v1.1
prunus_persica	Prupe.2G234700_v2.0.a1
pyrus_communis	pycom01g15440 pycom07g18510
rosa_chinensis	RchiOBHm_Chr1g0367031
rosa_laevigata	RLG00000027305
rosa_multiflora	Rmu_sc0013700.1_g000003
rosa_roxburghii	Rroxscaffold_4G00289690
rosa_rugosa	Rorug01G0334100
rosa_samantha	Rh1AG340800 Rh1BG301300 Rh1CG317500 Rh1DG333700
rosa_wichuraiana	Rw0G023880 Rw1G030270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	792
AccII	CGCG	1 cut(s)	22
AccIII	TCCGGA	1 cut(s)	624
AciI	CCGC	6 cut(s)	22, 48, 136, 219, 683, 976
AclWI	GGATC	5 cut(s)	169, 307, 531, 811, 1033
AcoI	YGGCCR	2 cut(s)	28, 454
AcvI	CACGTG	1 cut(s)	824
AfaI	GTAC	1 cut(s)	518
AfiI	CCNNNNNNNGG	1 cut(s)	241
AflII	CTTAAG	1 cut(s)	983
AflIII	ACRYGT	1 cut(s)	848
AgsI	TTSAA	5 cut(s)	250, 364, 430, 838, 892
AjnI	CCWGG	1 cut(s)	157
AjuI	GAANNNNNNNTTGG	2 cut(s)	435, 467
AluBI	AGCT	5 cut(s)	58, 80, 189, 648, 665
AluI	AGCT	5 cut(s)	58, 80, 189, 648, 665
Alw21I	GWGCWC	1 cut(s)	60
Alw26I	GTCTC	3 cut(s)	206, 363, 488
AlwI	GGATC	5 cut(s)	169, 307, 531, 811, 1033
AlwNI	CAGNNNCTG	2 cut(s)	80, 914
Aor13HI	TCCGGA	1 cut(s)	624
AoxI	GGCC	6 cut(s)	28, 49, 173, 304, 454, 960
ApaI	GGGCCC	2 cut(s)	308, 964
ApeKI	GCWGC	1 cut(s)	938
ArsI	GACNNNNNNTTYG	1 cut(s)	33
AspA2I	CCTAGG	2 cut(s)	256, 476
AspLEI	GCGC	1 cut(s)	556
AspS9I	GGNCC	6 cut(s)	253, 260, 304, 305, 960, 961
AsuHPI	GGTGA	4 cut(s)	19, 597, 671, 1048
AvaII	GGWCC	2 cut(s)	253, 260
AvrII	CCTAGG	2 cut(s)	256, 476
BaeGI	GKGCMC	2 cut(s)	308, 964
BaeI	ACNNNNGTAYC	2 cut(s)	1004, 1037
BanII	GRGCYC	3 cut(s)	60, 308, 964
BbrPI	CACGTG	1 cut(s)	824
BbsI	GAAGAC	3 cut(s)	478, 540, 1018
Bbv12I	GWGCWC	1 cut(s)	60
BbvI	GCAGC	1 cut(s)	925
BccI	CCATC	3 cut(s)	332, 791, 1026
BceAI	ACGGC	2 cut(s)	963, 1015
BciT130I	CCWGG	1 cut(s)	159
BciVI	GTATCC	1 cut(s)	1032
BcoDI	GTCTC	3 cut(s)	206, 363, 488
BfaI	CTAG	3 cut(s)	257, 477, 567
BfmI	CTRYAG	3 cut(s)	86, 864, 1055
BfrI	CTTAAG	1 cut(s)	983
BfuAI	ACCTGC	1 cut(s)	792
BfuI	GTATCC	1 cut(s)	1032
BglII	AGATCT	1 cut(s)	870
BisI	GCNGC	3 cut(s)	49, 137, 939
BlnI	CCTAGG	2 cut(s)	256, 476
BlpI	GCTNAGC	1 cut(s)	59
BlsI	GCNGC	3 cut(s)	50, 138, 940
BmcAI	AGTACT	1 cut(s)	518
Bme1390I	CCNGG	1 cut(s)	159
Bme18I	GGWCC	2 cut(s)	253, 260
BmgT120I	GGNCC	6 cut(s)	253, 260, 304, 305, 960, 961
BmiI	GGNNCC	3 cut(s)	306, 782, 962
BmrFI	CCNGG	1 cut(s)	159
BmsI	GCATC	1 cut(s)	82
BoxI	GACNNNNGTC	1 cut(s)	258
BpiI	GAAGAC	3 cut(s)	478, 540, 1018
Bpu10I	CCTNAGC	1 cut(s)	185
Bpu1102I	GCTNAGC	1 cut(s)	59
BpuEI	CTTGAG	1 cut(s)	74
BsaAI	YACGTR	1 cut(s)	824
BsaI	GGTCTC	1 cut(s)	363
BsaJI	CCNNGG	2 cut(s)	256, 476
BsaWI	WCCGGW	1 cut(s)	624
BsaXI	ACNNNNNCTCC	4 cut(s)	360, 390, 637, 667
Bsc4I	CCNNNNNNNGG	1 cut(s)	241
Bse118I	RCCGGY	1 cut(s)	175
Bse1I	ACTGG	1 cut(s)	416
Bse3DI	GCAATG	1 cut(s)	563
BseAI	TCCGGA	1 cut(s)	624
BseBI	CCWGG	1 cut(s)	159
BseDI	CCNNGG	2 cut(s)	256, 476
BseGI	GGATG	2 cut(s)	97, 378
BseLI	CCNNNNNNNGG	1 cut(s)	241
BseMI	GCAATG	1 cut(s)	563
BseMII	CTCAG	2 cut(s)	73, 240
BseNI	ACTGG	1 cut(s)	416
BseSI	GKGCMC	2 cut(s)	308, 964
BseXI	GCAGC	1 cut(s)	925
Bsh1236I	CGCG	1 cut(s)	22
BshFI	GGCC	6 cut(s)	30, 51, 175, 306, 456, 962
BsiHKAI	GWGCWC	1 cut(s)	60
BsiSI	CCGG	2 cut(s)	176, 625
BslI	CCNNNNNNNGG	1 cut(s)	241
BsmAI	GTCTC	3 cut(s)	206, 363, 488
BsnI	GGCC	6 cut(s)	30, 51, 175, 306, 456, 962
Bso31I	GGTCTC	1 cut(s)	363
Bsp120I	GGGCCC	2 cut(s)	304, 960
Bsp1286I	GDGCHC	3 cut(s)	60, 308, 964
Bsp13I	TCCGGA	1 cut(s)	624
Bsp143I	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
Bsp1720I	GCTNAGC	1 cut(s)	59
BspACI	CCGC	6 cut(s)	22, 48, 136, 219, 683, 976
BspANI	GGCC	6 cut(s)	30, 51, 175, 306, 456, 962
BspCNI	CTCAG	2 cut(s)	72, 239
BspEI	TCCGGA	1 cut(s)	624
BspFNI	CGCG	1 cut(s)	22
BspHI	TCATGA	1 cut(s)	948
BspLI	GGNNCC	3 cut(s)	306, 782, 962
BspMAI	CTGCAG	1 cut(s)	90
BspMI	ACCTGC	1 cut(s)	792
BspPI	GGATC	5 cut(s)	169, 307, 531, 811, 1033
BspTI	CTTAAG	1 cut(s)	983
BspTNI	GGTCTC	1 cut(s)	363
BsrDI	GCAATG	1 cut(s)	563
BsrFI	RCCGGY	1 cut(s)	175
BsrI	ACTGG	1 cut(s)	416
BssAI	RCCGGY	1 cut(s)	175
BssECI	CCNNGG	2 cut(s)	256, 476
BssMI	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
BssT1I	CCWWGG	2 cut(s)	256, 476
Bst2UI	CCWGG	1 cut(s)	159
Bst4CI	ACNGT	5 cut(s)	13, 76, 241, 374, 910
Bst6I	CTCTTC	3 cut(s)	191, 290, 486
BstAFI	CTTAAG	1 cut(s)	983
BstBAI	YACGTR	1 cut(s)	824
BstC8I	GCNNGC	1 cut(s)	916
BstDEI	CTNAG	5 cut(s)	59, 185, 226, 500, 511
BstEII	GGTNACC	1 cut(s)	7
BstF5I	GGATG	2 cut(s)	97, 378
BstFNI	CGCG	1 cut(s)	22
BstHHI	GCGC	1 cut(s)	556
BstKTI	GATC	7 cut(s)	85, 164, 315, 526, 819, 873, 1041
BstMAI	GTCTC	3 cut(s)	206, 363, 488
BstMBI	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
BstMWI	GCNNNNNNNGC	2 cut(s)	77, 662
BstNI	CCWGG	1 cut(s)	159
BstNSI	RCATGY	1 cut(s)	852
BstPAI	GACNNNNGTC	1 cut(s)	258
BstPI	GGTNACC	1 cut(s)	7
BstSCI	CCNGG	1 cut(s)	157
BstSFI	CTRYAG	3 cut(s)	86, 864, 1055
BstSLI	GKGCMC	2 cut(s)	308, 964
BstUI	CGCG	1 cut(s)	22
BstV1I	GCAGC	1 cut(s)	925
BstV2I	GAAGAC	3 cut(s)	478, 540, 1018
BstX2I	RGATCY	2 cut(s)	816, 870
BstXI	CCANNNNNNTGG	1 cut(s)	1032
BstYI	RGATCY	2 cut(s)	816, 870
BsuI	GTATCC	1 cut(s)	1032
BsuRI	GGCC	6 cut(s)	30, 51, 175, 306, 456, 962
BtsCI	GGATG	2 cut(s)	97, 378
BtsIMutI	CAGTG	1 cut(s)	884
BveI	ACCTGC	1 cut(s)	792
Cac8I	GCNNGC	1 cut(s)	916
CaiI	CAGNNNCTG	2 cut(s)	80, 914
CciI	TCATGA	1 cut(s)	948
CfoI	GCGC	1 cut(s)	556
Cfr10I	RCCGGY	1 cut(s)	175
Cfr13I	GGNCC	6 cut(s)	253, 260, 304, 305, 960, 961
Csp6I	GTAC	1 cut(s)	517
CviAII	CATG	4 cut(s)	538, 596, 849, 949
CviQI	GTAC	1 cut(s)	517
DdeI	CTNAG	5 cut(s)	59, 185, 226, 500, 511
DpnI	GATC	7 cut(s)	84, 163, 314, 525, 818, 872, 1040
DpnII	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
EaeI	YGGCCR	2 cut(s)	28, 454
Eam1104I	CTCTTC	3 cut(s)	191, 290, 486
EarI	CTCTTC	3 cut(s)	191, 290, 486
Ecl136II	GAGCTC	1 cut(s)	58
Eco130I	CCWWGG	2 cut(s)	256, 476
Eco24I	GRGCYC	3 cut(s)	60, 308, 964
Eco31I	GGTCTC	1 cut(s)	363
Eco32I	GATATC	1 cut(s)	812
Eco47I	GGWCC	2 cut(s)	253, 260
Eco53kI	GAGCTC	1 cut(s)	58
Eco72I	CACGTG	1 cut(s)	824
Eco91I	GGTNACC	1 cut(s)	7
EcoICRI	GAGCTC	1 cut(s)	58
EcoO109I	RGGNCCY	3 cut(s)	253, 305, 961
EcoO65I	GGTNACC	1 cut(s)	7
EcoRII	CCWGG	1 cut(s)	157
EcoRV	GATATC	1 cut(s)	812
EcoT14I	CCWWGG	2 cut(s)	256, 476
EcoT38I	GRGCYC	3 cut(s)	60, 308, 964
ErhI	CCWWGG	2 cut(s)	256, 476
FaeI	CATG	4 cut(s)	541, 599, 852, 952
FatI	CATG	4 cut(s)	537, 595, 848, 948
FauI	CCCGC	1 cut(s)	690
FauNDI	CATATG	1 cut(s)	397
Fnu4HI	GCNGC	3 cut(s)	49, 137, 939
FokI	GGATG	2 cut(s)	104, 365
FriOI	GRGCYC	3 cut(s)	60, 308, 964
Fsp4HI	GCNGC	3 cut(s)	49, 137, 939
FspBI	CTAG	3 cut(s)	257, 477, 567
GlaI	GCGC	1 cut(s)	555
GluI	GCNGC	3 cut(s)	49, 137, 939
HaeIII	GGCC	6 cut(s)	30, 51, 175, 306, 456, 962
HapII	CCGG	2 cut(s)	176, 625
HhaI	GCGC	1 cut(s)	556
Hin1II	CATG	4 cut(s)	541, 599, 852, 952
Hin6I	GCGC	1 cut(s)	554
HinP1I	GCGC	1 cut(s)	554
HinfI	GANTC	5 cut(s)	65, 199, 496, 571, 676
HpaII	CCGG	2 cut(s)	176, 625
HphI	GGTGA	4 cut(s)	19, 597, 671, 1048
Hpy166II	GTNNAC	2 cut(s)	263, 346
Hpy188I	TCNGA	2 cut(s)	229, 816
Hpy188III	TCNNGA	3 cut(s)	391, 625, 949
Hpy8I	GTNNAC	2 cut(s)	263, 346
HpyAV	CCTTC	1 cut(s)	453
HpyCH4III	ACNGT	5 cut(s)	13, 76, 241, 374, 910
HpyCH4IV	ACGT	2 cut(s)	433, 823
HpyCH4V	TGCA	5 cut(s)	88, 95, 128, 599, 854
HpyF10VI	GCNNNNNNNGC	2 cut(s)	77, 662
HpyF3I	CTNAG	5 cut(s)	59, 185, 226, 500, 511
HpySE526I	ACGT	2 cut(s)	433, 823
Hsp92II	CATG	4 cut(s)	541, 599, 852, 952
HspAI	GCGC	1 cut(s)	554
Kpn2I	TCCGGA	1 cut(s)	624
Kzo9I	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
LmnI	GCTCC	1 cut(s)	645
Lsp1109I	GCAGC	1 cut(s)	925
LweI	GCATC	1 cut(s)	82
MaeI	CTAG	3 cut(s)	257, 477, 567
MaeII	ACGT	2 cut(s)	433, 823
MaeIII	GTNAC	3 cut(s)	7, 701, 844
MalI	GATC	7 cut(s)	84, 163, 314, 525, 818, 872, 1040
MboI	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
MboII	GAAGA	9 cut(s)	208, 277, 280, 483, 503, 545, 727, 1000, 1023
MfeI	CAATTG	1 cut(s)	955
MflI	RGATCY	2 cut(s)	816, 870
MhlI	GDGCHC	3 cut(s)	60, 308, 964
MluCI	AATT	3 cut(s)	628, 856, 955
MlyI	GAGTC	2 cut(s)	208, 490
MmeI	TCCRAC	1 cut(s)	178
MnlI	CCTC	7 cut(s)	165, 461, 613, 716, 771, 800, 1056
MroI	TCCGGA	1 cut(s)	624
MseI	TTAA	1 cut(s)	984
MslI	CAYNNNNRTG	1 cut(s)	1030
MspA1I	CMGCKG	2 cut(s)	136, 219
MspCI	CTTAAG	1 cut(s)	983
MspI	CCGG	2 cut(s)	176, 625
MspR9I	CCNGG	1 cut(s)	159
MunI	CAATTG	1 cut(s)	955
MvaI	CCWGG	1 cut(s)	159
MvnI	CGCG	1 cut(s)	22
MwoI	GCNNNNNNNGC	2 cut(s)	77, 662
NdeI	CATATG	1 cut(s)	397
NdeII	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
NlaIII	CATG	4 cut(s)	541, 599, 852, 952
NlaIV	GGNNCC	3 cut(s)	306, 782, 962
NmeAIII	GCCGAG	1 cut(s)	56
NmuCI	GTSAC	3 cut(s)	7, 701, 844
NspI	RCATGY	1 cut(s)	852
PagI	TCATGA	1 cut(s)	948
PciI	ACATGT	1 cut(s)	848
PfeI	GAWTC	3 cut(s)	65, 571, 676
PflFI	GACNNNGTC	1 cut(s)	374
PkrI	GCNGC	3 cut(s)	50, 138, 940
PleI	GAGTC	2 cut(s)	207, 490
PmaCI	CACGTG	1 cut(s)	824
PmlI	CACGTG	1 cut(s)	824
PpsI	GAGTC	2 cut(s)	207, 490
Ppu21I	YACGTR	1 cut(s)	824
PpuMI	RGGWCCY	1 cut(s)	253
PscI	ACATGT	1 cut(s)	848
PshAI	GACNNNNGTC	1 cut(s)	258
Psp124BI	GAGCTC	1 cut(s)	60
Psp5II	RGGWCCY	1 cut(s)	253
Psp6I	CCWGG	1 cut(s)	157
PspCI	CACGTG	1 cut(s)	824
PspEI	GGTNACC	1 cut(s)	7
PspGI	CCWGG	1 cut(s)	157
PspN4I	GGNNCC	3 cut(s)	306, 782, 962
PspOMI	GGGCCC	2 cut(s)	304, 960
PspPI	GGNCC	6 cut(s)	253, 260, 304, 305, 960, 961
PspPPI	RGGWCCY	1 cut(s)	253
PstI	CTGCAG	1 cut(s)	90
PstNI	CAGNNNCTG	2 cut(s)	80, 914
PsuI	RGATCY	2 cut(s)	816, 870
PsyI	GACNNNGTC	1 cut(s)	374
RsaI	GTAC	1 cut(s)	518
RsaNI	GTAC	1 cut(s)	517
RseI	CAYNNNNRTG	1 cut(s)	1030
SacI	GAGCTC	1 cut(s)	60
SaqAI	TTAA	1 cut(s)	984
SatI	GCNGC	3 cut(s)	49, 137, 939
Sau3AI	GATC	7 cut(s)	82, 161, 312, 523, 816, 870, 1038
Sau96I	GGNCC	6 cut(s)	253, 260, 304, 305, 960, 961
ScaI	AGTACT	1 cut(s)	518
SchI	GAGTC	2 cut(s)	208, 490
ScrFI	CCNGG	1 cut(s)	159
SduI	GDGCHC	3 cut(s)	60, 308, 964
SfaNI	GCATC	1 cut(s)	82
SfcI	CTRYAG	3 cut(s)	86, 864, 1055
SinI	GGWCC	2 cut(s)	253, 260
SmiMI	CAYNNNNRTG	1 cut(s)	1030
SmlI	CTYRAG	2 cut(s)	53, 983
SmoI	CTYRAG	2 cut(s)	53, 983
Sse9I	AATT	3 cut(s)	628, 856, 955
SsiI	CCGC	6 cut(s)	22, 48, 136, 219, 683, 976
SspI	AATATT	1 cut(s)	841
SspMI	CTAG	3 cut(s)	257, 477, 567
SstI	GAGCTC	1 cut(s)	60
StyD4I	CCNGG	1 cut(s)	157
StyI	CCWWGG	2 cut(s)	256, 476
TaaI	ACNGT	5 cut(s)	13, 76, 241, 374, 910
TaiI	ACGT	2 cut(s)	436, 826
TaqI	TCGA	1 cut(s)	526
TasI	AATT	3 cut(s)	628, 856, 955
TatI	WGTACW	1 cut(s)	516
TauI	GCSGC	2 cut(s)	51, 139
TfiI	GAWTC	3 cut(s)	65, 571, 676
Tru1I	TTAA	1 cut(s)	984
Tru9I	TTAA	1 cut(s)	984
TscAI	CASTG	1 cut(s)	884
TseFI	GTSAC	3 cut(s)	7, 701, 844
TseI	GCWGC	1 cut(s)	938
Tsp45I	GTSAC	3 cut(s)	7, 701, 844
TspDTI	ATGAA	4 cut(s)	17, 563, 649, 728
TspGWI	ACGGA	2 cut(s)	545, 1023
TspRI	CASTG	1 cut(s)	884
Tth111I	GACNNNGTC	1 cut(s)	374
Vha464I	CTTAAG	1 cut(s)	983
VpaK11BI	GGWCC	2 cut(s)	253, 260
XceI	RCATGY	1 cut(s)	852
XcmI	CCANNNNNNNNNTGG	1 cut(s)	716
XmaJI	CCTAGG	2 cut(s)	256, 476
XspI	CTAG	3 cut(s)	257, 477, 567
ZrmI	AGTACT	1 cut(s)	518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.