Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0013166

Physical Location & Seq

Genomic Coordinates Forward (+)

55974145 .. 55977622

Gene Structure 3478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG333700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 843 bp

ATGATTAAATCTCTTATGTCAACAGCCAAAAAGTTCTATCCTTCAAGGCAGCGGCTTACTCTTCCTGTCCAACCAGGATCAAAAGAGAAGGCAGTTGTCCTCGCCTCCAAGAAGAGTCTCAAAGATTATTACAGTGGAAACTCAGACAACTTAACTGTGGTGTTCAAGGACTTAGGCCCACAAGTTGAATACCGAACGCTCTTCTTCTTTGAGTATATTGGTCCTTTGCTCATTTATCCCATATTTTACTTCTTCCCTGTTTACCAGTACTTGGGCTTCAAAGGAGACCATGTCATCCACCCAGTCCAGACATATGCTCTATATTATTATTGCTTCCACTACTTTAAACGTATTATGGAAACATTTTTCATACATAGGTTCAGCCATGCAACCTCACCTCTCTCCTATGTATCCCGGAATTGTGCTTATTATTGGAGCTTTGCAGCACTCATGTCTTACTACGTGAATCACCCGCTTTGCACCCCTGTTAGCGACCTCCAAATGAAGATTGGCTTTGGTTTTGGGATAATATGTCAAATTGCGAACTTGTATTGTCATATTCTATTGAGGAATCTACGAAGCCCTGTTGGAAGTGGAGGCTATCAAATACCACGTGGTTTTCTATTCAATATTGTGACATGCGCAAATTATACAACTGAGATTTATCAGTGGTTGGGATTTAATATTGCAACGCAGACGGTTGCTGGTTATCTTTTTGCTGTAGTTGCTGCTTCTATAATGACCAATTGGGCCCTCGGAAAGCACCGCCGTTTAAAGAAGGTACTCCTCTATACTACTGTCCTCCAAACCAAATATGTTGTTTGGATTGCTAATAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004312 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006643 GO:0006665 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009888 GO:0009922 GO:0009923 GO:0009987 GO:0010025 GO:0010026 GO:0010090 GO:0010091 GO:0010166 GO:0010927 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016053 GO:0016491 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030176 GO:0030497 GO:0031224 GO:0031227 GO:0031984 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0032991 GO:0042175 GO:0042335 GO:0042761 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0055114 GO:0071704 GO:0071840 GO:0071944 GO:0072330 GO:0090558 GO:0090626 GO:0098796 GO:0098827 GO:1901564 GO:1901568 GO:1901570 GO:1901576 GO:1902494 GO:1905499 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

280

Amino Acids

32.44

Weight (kDa)

9.56

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	131 - 260	8.3e-23	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55360
fragaria_vesca	FvH4_7g22400
malus_domestica	MD01G1133600.v1.1 MD07G1197900.v1.1
prunus_persica	Prupe.2G234700_v2.0.a1
pyrus_communis	pycom01g15440 pycom07g18510
rosa_chinensis	RchiOBHm_Chr1g0367031
rosa_laevigata	RLG00000027305
rosa_multiflora	Rmu_sc0013700.1_g000003
rosa_roxburghii	Rroxscaffold_4G00289690
rosa_rugosa	Rorug01G0334100
rosa_samantha	Rh1AG340800 Rh1BG301300 Rh1CG317500 Rh1DG333700
rosa_wichuraiana	Rw0G023880 Rw1G030270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	643
AccB7I	CCANNNNNTGG	1 cut(s)	271
AciI	CCGC	3 cut(s)	52, 473, 766
AclWI	GGATC	1 cut(s)	85
AcvI	CACGTG	1 cut(s)	614
AfaI	GTAC	2 cut(s)	269, 783
AfiI	CCNNNNNNNGG	1 cut(s)	271
AgsI	TTSAA	5 cut(s)	45, 166, 188, 280, 628
AjnI	CCWGG	1 cut(s)	73
AluBI	AGCT	1 cut(s)	438
AluI	AGCT	1 cut(s)	438
Alw26I	GTCTC	2 cut(s)	122, 279
AlwI	GGATC	1 cut(s)	85
AoxI	GGCC	2 cut(s)	175, 750
ApaI	GGGCCC	1 cut(s)	754
ApeKI	GCWGC	3 cut(s)	49, 443, 728
AspLEI	GCGC	1 cut(s)	644
AspS9I	GGNCC	4 cut(s)	176, 221, 750, 751
AsuC2I	CCSGG	1 cut(s)	415
AsuHPI	GGTGA	2 cut(s)	387, 461
AvaII	GGWCC	1 cut(s)	221
BaeGI	GKGCMC	1 cut(s)	754
BanII	GRGCYC	1 cut(s)	754
BarI	GAAGNNNNNNTAC	2 cut(s)	260, 292
BbrPI	CACGTG	1 cut(s)	614
BbvI	GCAGC	3 cut(s)	61, 455, 715
BceAI	ACGGC	1 cut(s)	753
BciT130I	CCWGG	1 cut(s)	75
BciVI	GTATCC	1 cut(s)	421
BcnI	CCSGG	1 cut(s)	415
BcoDI	GTCTC	2 cut(s)	122, 279
BfmI	CTRYAG	1 cut(s)	720
BfuI	GTATCC	1 cut(s)	421
BisI	GCNGC	4 cut(s)	50, 53, 444, 729
BlsI	GCNGC	4 cut(s)	51, 54, 445, 730
BmcAI	AGTACT	1 cut(s)	269
Bme1390I	CCNGG	2 cut(s)	75, 415
Bme18I	GGWCC	1 cut(s)	221
BmgT120I	GGNCC	4 cut(s)	176, 221, 750, 751
BmiI	GGNNCC	1 cut(s)	752
BmrFI	CCNGG	2 cut(s)	75, 415
BmrI	ACTGGG	1 cut(s)	296
BmuI	ACTGGG	1 cut(s)	296
BpuMI	CCSGG	1 cut(s)	415
BsaAI	YACGTR	2 cut(s)	463, 614
BsaI	GGTCTC	1 cut(s)	279
BsaJI	CCNNGG	1 cut(s)	754
BsaXI	ACNNNNNCTCC	2 cut(s)	276, 306
Bsc4I	CCNNNNNNNGG	1 cut(s)	271
Bse1I	ACTGG	2 cut(s)	265, 302
BseBI	CCWGG	1 cut(s)	75
BseDI	CCNNGG	1 cut(s)	754
BseGI	GGATG	1 cut(s)	294
BseLI	CCNNNNNNNGG	1 cut(s)	271
BseMII	CTCAG	2 cut(s)	156, 648
BseNI	ACTGG	2 cut(s)	265, 302
BseRI	GAGGAG	1 cut(s)	776
BseSI	GKGCMC	1 cut(s)	754
BseXI	GCAGC	3 cut(s)	61, 455, 715
BshFI	GGCC	2 cut(s)	177, 752
BsiSI	CCGG	1 cut(s)	415
BslI	CCNNNNNNNGG	1 cut(s)	271
BsmAI	GTCTC	2 cut(s)	122, 279
BsnI	GGCC	2 cut(s)	177, 752
Bso31I	GGTCTC	1 cut(s)	279
Bsp120I	GGGCCC	1 cut(s)	750
Bsp1286I	GDGCHC	1 cut(s)	754
Bsp143I	GATC	1 cut(s)	77
BspACI	CCGC	3 cut(s)	52, 473, 766
BspANI	GGCC	2 cut(s)	177, 752
BspCNI	CTCAG	2 cut(s)	155, 649
BspLI	GGNNCC	1 cut(s)	752
BspPI	GGATC	1 cut(s)	85
BspQI	GCTCTTC	1 cut(s)	206
BspTNI	GGTCTC	1 cut(s)	279
BsrI	ACTGG	2 cut(s)	265, 302
BssECI	CCNNGG	1 cut(s)	754
BssMI	GATC	1 cut(s)	77
Bst2UI	CCWGG	1 cut(s)	75
Bst4CI	ACNGT	4 cut(s)	134, 157, 700, 799
Bst6I	CTCTTC	3 cut(s)	66, 107, 206
BstBAI	YACGTR	2 cut(s)	463, 614
BstDEI	CTNAG	3 cut(s)	142, 172, 657
BstF5I	GGATG	1 cut(s)	294
BstHHI	GCGC	1 cut(s)	644
BstKTI	GATC	1 cut(s)	80
BstMAI	GTCTC	2 cut(s)	122, 279
BstMBI	GATC	1 cut(s)	77
BstMWI	GCNNNNNNNGC	1 cut(s)	725
BstNI	CCWGG	1 cut(s)	75
BstNSI	RCATGY	1 cut(s)	642
BstSCI	CCNGG	2 cut(s)	73, 413
BstSFI	CTRYAG	1 cut(s)	720
BstSLI	GKGCMC	1 cut(s)	754
BstV1I	GCAGC	3 cut(s)	61, 455, 715
BsuI	GTATCC	1 cut(s)	421
BsuRI	GGCC	2 cut(s)	177, 752
BtsCI	GGATG	1 cut(s)	294
BtsIMutI	CAGTG	2 cut(s)	139, 674
CfoI	GCGC	1 cut(s)	644
Cfr13I	GGNCC	4 cut(s)	176, 221, 750, 751
Csp6I	GTAC	2 cut(s)	268, 782
CviAII	CATG	4 cut(s)	290, 386, 451, 639
CviQI	GTAC	2 cut(s)	268, 782
DdeI	CTNAG	3 cut(s)	142, 172, 657
DpnI	GATC	1 cut(s)	79
DpnII	GATC	1 cut(s)	77
DraI	TTTAAA	2 cut(s)	346, 774
Eam1104I	CTCTTC	3 cut(s)	66, 107, 206
EarI	CTCTTC	3 cut(s)	66, 107, 206
Eco24I	GRGCYC	1 cut(s)	754
Eco31I	GGTCTC	1 cut(s)	279
Eco47I	GGWCC	1 cut(s)	221
Eco72I	CACGTG	1 cut(s)	614
EcoO109I	RGGNCCY	1 cut(s)	751
EcoRII	CCWGG	1 cut(s)	73
EcoT38I	GRGCYC	1 cut(s)	754
FaeI	CATG	4 cut(s)	293, 389, 454, 642
FalI	AAGNNNNNCTT	2 cut(s)	497, 529
FatI	CATG	4 cut(s)	289, 385, 450, 638
FauI	CCCGC	1 cut(s)	480
FauNDI	CATATG	1 cut(s)	313
Fnu4HI	GCNGC	4 cut(s)	50, 53, 444, 729
FokI	GGATG	1 cut(s)	281
FriOI	GRGCYC	1 cut(s)	754
Fsp4HI	GCNGC	4 cut(s)	50, 53, 444, 729
FspI	TGCGCA	1 cut(s)	643
GlaI	GCGC	1 cut(s)	643
GluI	GCNGC	4 cut(s)	50, 53, 444, 729
HaeIII	GGCC	2 cut(s)	177, 752
HapII	CCGG	1 cut(s)	415
HhaI	GCGC	1 cut(s)	644
Hin1II	CATG	4 cut(s)	293, 389, 454, 642
Hin6I	GCGC	1 cut(s)	642
HinP1I	GCGC	1 cut(s)	642
HincII	GTYRAC	1 cut(s)	21
HindII	GTYRAC	1 cut(s)	21
HinfI	GANTC	3 cut(s)	115, 466, 571
HpaII	CCGG	1 cut(s)	415
HphI	GGTGA	2 cut(s)	387, 461
Hpy166II	GTNNAC	2 cut(s)	21, 262
Hpy188I	TCNGA	2 cut(s)	145, 758
Hpy188III	TCNNGA	1 cut(s)	307
Hpy8I	GTNNAC	2 cut(s)	21, 262
HpyAV	CCTTC	3 cut(s)	51, 82, 772
HpyCH4III	ACNGT	4 cut(s)	134, 157, 700, 799
HpyCH4IV	ACGT	3 cut(s)	349, 462, 613
HpyCH4V	TGCA	4 cut(s)	389, 443, 480, 689
HpyF10VI	GCNNNNNNNGC	1 cut(s)	725
HpyF3I	CTNAG	3 cut(s)	142, 172, 657
HpySE526I	ACGT	3 cut(s)	349, 462, 613
Hsp92II	CATG	4 cut(s)	293, 389, 454, 642
HspAI	GCGC	1 cut(s)	642
Kzo9I	GATC	1 cut(s)	77
LguI	GCTCTTC	1 cut(s)	206
LmnI	GCTCC	1 cut(s)	435
Lsp1109I	GCAGC	3 cut(s)	61, 455, 715
MaeII	ACGT	3 cut(s)	349, 462, 613
MaeIII	GTNAC	1 cut(s)	634
MalI	GATC	1 cut(s)	79
MboI	GATC	1 cut(s)	77
MboII	GAAGA	6 cut(s)	53, 124, 193, 196, 244, 517
MfeI	CAATTG	1 cut(s)	745
MhlI	GDGCHC	1 cut(s)	754
MluCI	AATT	5 cut(s)	418, 537, 646, 745, 835
MlyI	GAGTC	1 cut(s)	124
MmeI	TCCRAC	2 cut(s)	94, 568
MseI	TTAA	5 cut(s)	6, 152, 345, 681, 773
MspA1I	CMGCKG	1 cut(s)	52
MspI	CCGG	1 cut(s)	415
MspR9I	CCNGG	2 cut(s)	75, 415
MunI	CAATTG	1 cut(s)	745
MvaI	CCWGG	1 cut(s)	75
MwoI	GCNNNNNNNGC	1 cut(s)	725
NciI	CCSGG	1 cut(s)	415
NdeI	CATATG	1 cut(s)	313
NdeII	GATC	1 cut(s)	77
NlaIII	CATG	4 cut(s)	293, 389, 454, 642
NlaIV	GGNNCC	1 cut(s)	752
NmuCI	GTSAC	1 cut(s)	634
NsbI	TGCGCA	1 cut(s)	643
NspI	RCATGY	1 cut(s)	642
PciSI	GCTCTTC	1 cut(s)	206
PfeI	GAWTC	2 cut(s)	466, 571
PflFI	GACNNNGTC	1 cut(s)	290
PflMI	CCANNNNNTGG	1 cut(s)	271
PfoI	TCCNGGA	1 cut(s)	413
PkrI	GCNGC	4 cut(s)	51, 54, 445, 730
PleI	GAGTC	1 cut(s)	123
PmaCI	CACGTG	1 cut(s)	614
PmlI	CACGTG	1 cut(s)	614
PpsI	GAGTC	1 cut(s)	123
Ppu21I	YACGTR	2 cut(s)	463, 614
Psp6I	CCWGG	1 cut(s)	73
PspCI	CACGTG	1 cut(s)	614
PspGI	CCWGG	1 cut(s)	73
PspN4I	GGNNCC	1 cut(s)	752
PspOMI	GGGCCC	1 cut(s)	750
PspPI	GGNCC	4 cut(s)	176, 221, 750, 751
PsyI	GACNNNGTC	1 cut(s)	290
RsaI	GTAC	2 cut(s)	269, 783
RsaNI	GTAC	2 cut(s)	268, 782
SapI	GCTCTTC	1 cut(s)	206
SaqAI	TTAA	5 cut(s)	6, 152, 345, 681, 773
SatI	GCNGC	4 cut(s)	50, 53, 444, 729
Sau3AI	GATC	1 cut(s)	77
Sau96I	GGNCC	4 cut(s)	176, 221, 750, 751
ScaI	AGTACT	1 cut(s)	269
SchI	GAGTC	1 cut(s)	124
ScrFI	CCNGG	2 cut(s)	75, 415
SduI	GDGCHC	1 cut(s)	754
SetI	ASST	9 cut(s)	352, 380, 395, 400, 440, 465, 498, 616, 783
SfcI	CTRYAG	1 cut(s)	720
SinI	GGWCC	1 cut(s)	221
Sse9I	AATT	5 cut(s)	418, 537, 646, 745, 835
SsiI	CCGC	3 cut(s)	52, 473, 766
SspI	AATATT	2 cut(s)	631, 685
StyD4I	CCNGG	2 cut(s)	73, 413
TaaI	ACNGT	4 cut(s)	134, 157, 700, 799
TaiI	ACGT	3 cut(s)	352, 465, 616
TasI	AATT	5 cut(s)	418, 537, 646, 745, 835
TatI	WGTACW	1 cut(s)	267
TauI	GCSGC	1 cut(s)	55
TfiI	GAWTC	2 cut(s)	466, 571
Tru1I	TTAA	5 cut(s)	6, 152, 345, 681, 773
Tru9I	TTAA	5 cut(s)	6, 152, 345, 681, 773
TscAI	CASTG	2 cut(s)	139, 674
TseFI	GTSAC	1 cut(s)	634
TseI	GCWGC	3 cut(s)	49, 443, 728
Tsp45I	GTSAC	1 cut(s)	634
TspDTI	ATGAA	2 cut(s)	358, 518
TspRI	CASTG	2 cut(s)	139, 674
Tth111I	GACNNNGTC	1 cut(s)	290
Van91I	CCANNNNNTGG	1 cut(s)	271
VpaK11BI	GGWCC	1 cut(s)	221
XceI	RCATGY	1 cut(s)	642
XcmI	CCANNNNNNNNNTGG	1 cut(s)	506
ZrmI	AGTACT	1 cut(s)	269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.