Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010173

Physical Location & Seq

Genomic Coordinates Reverse (-)

26193698 .. 26196298

Gene Structure 2601 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1153600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGAGTTGCCCACAGGATTGGCCAGAGCCAATAGTCCGAGTACAATCCTTATCCAACAGCCCCGTAGTCCCAGATCGATACATCAAACCCCCACCTGATAGACCCACCTATATCCCATCATCCGATATTTCTGATCATGCCAACATCCCAGTTATTGACTTACAAGGCTTATCCGGCGAAGACGATCGCCTGCGTGCCGCCACGCTTAGCCAAATCTCCGAGGCCTGCCGTGAGTGGGGATTCTTCCAAGTGGTGAACCACGGCGTTAGCCCGGGACTGATGGACAAAACCCAACAGGTTTGGCGGGAATTCTTCCACTTGCCGATGGAGTTGAAACAGGCGTATGCCAACTCACCAAAGACTTATGAAGGGTATGGGAGCAGGCTTGGAATTGAGAGAGGCGCCATTCTTGACTGGAGCGATTACTATTTCCTTCACTATCTTCCGTCGTGCTTAAAGGACTACAACAAGTGGCCTGCCCTCCCTGATTATGGAAGGGAAGTAATAGATGAGTATGGAAAGGAGGTAGTGAAGCTTGGTGGGAGGATATTGAAGGCTTTGTCACTAAACCTTGGATTAGGAGAAGACTTTCTTCAAAATGCATTTGGTGGCCAAGACATTGGGGCATGTCTAAGAACTAACTTCTATCCAAAGTGCCCCCAGCCTGACCTGACCCTTGGCCTGTCGTCGCACTCGGACCCCGGCGGAATGACCATTTTGCTCGCCGACCAACAAGTCCCAGGTCTACAAGTCCGGAAAGATGACGCTTGGATCACTGTGAAACCAGCTCCACACGCTTTTATTGTCAATATTGGGGATCAAATTCAGGTCTTAAGTAATGCAACGTACAAGAGTGTAGAGCACAGGGTCATCGTAAACCCAGCTAAAGAGAGACTCTCGTTGGCCTTCTTCTACAATCCGAAGAGCGACATACCCATTCAACCGGTGAAGGAGCTTGTAACGCCGGACAAGCCTGCACAATATTCTCCTATGACGTTCAATGAATACAGGCTTTACATAAGATTGAATGGCCCTCGAGGAAAATCTCAAGTCGAATCTTTAAAGTCTCCACGAAATACATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0009628 GO:0009636 GO:0009966 GO:0009987 GO:0010646 GO:0016787 GO:0016810 GO:0023051 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0080167 GO:0097237 GO:0120091 GO:2000022

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

40.59

Weight (kDa)

6.19

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	49 - 160	1.1e-32	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	209 - 307	2.5e-34	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010173)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05600 AT5G05600
fragaria_vesca	FvH4_7g24550 FvH4_7g24550 FvH4_7g24550
malus_domestica	MD01G1153600.v1.1 MD07G1222600.v1.1
prunus_persica	Prupe.2G250900_v2.0.a1
pyrus_communis	pycom01g17120 pycom07g20130
rosa_chinensis	RchiOBHm_Chr1g0370391
rosa_laevigata	RLG00000027052
rosa_multiflora	Rmu_sc0005656.1_g000002
rosa_roxburghii	Rroxscaffold_4G00286630
rosa_rugosa	Rorug01G0356100
rosa_samantha	Rh1AG364400 Rh1BG327700 Rh1CG340700 Rh1DG358000
rosa_wichuraiana	Rw1G032070 Rw1G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	734
AbsI	CCTCGAGG	1 cut(s)	1035
AccB1I	GGYRCC	1 cut(s)	401
AccI	GTMKAC	1 cut(s)	745
AccIII	TCCGGA	1 cut(s)	753
AciI	CCGC	3 cut(s)	198, 304, 705
AclWI	GGATC	2 cut(s)	779, 825
AcoI	YGGCCR	2 cut(s)	20, 610
AcsI	RAATTY	2 cut(s)	308, 822
AcyI	GRCGYC	1 cut(s)	402
AfaI	GTAC	2 cut(s)	42, 848
AfiI	CCNNNNNNNGG	2 cut(s)	235, 491
AflII	CTTAAG	1 cut(s)	832
AgeI	ACCGGT	1 cut(s)	943
AgsI	TTSAA	6 cut(s)	334, 553, 596, 941, 1000, 1027
AjnI	CCWGG	1 cut(s)	739
AluBI	AGCT	4 cut(s)	535, 788, 884, 955
AluI	AGCT	4 cut(s)	535, 788, 884, 955
Alw21I	GWGCWC	1 cut(s)	864
Alw26I	GTCTC	2 cut(s)	886, 1071
AlwI	GGATC	2 cut(s)	779, 825
Ama87I	CYCGRG	2 cut(s)	271, 1035
Aor13HI	TCCGGA	1 cut(s)	753
AoxI	GGCC	7 cut(s)	20, 222, 473, 610, 679, 903, 1030
ApoI	RAATTY	2 cut(s)	308, 822
AsiGI	ACCGGT	1 cut(s)	943
Asp700I	GAANNNNTTC	1 cut(s)	588
AspLEI	GCGC	1 cut(s)	404
AspS9I	GGNCC	2 cut(s)	697, 1031
AsuC2I	CCSGG	3 cut(s)	272, 273, 702
AsuHPI	GGTGA	3 cut(s)	265, 345, 958
AvaI	CYCGRG	2 cut(s)	271, 1035
AvaII	GGWCC	1 cut(s)	697
BaeGI	GKGCMC	1 cut(s)	659
BalI	TGGCCA	2 cut(s)	22, 612
BanI	GGYRCC	1 cut(s)	401
BbsI	GAAGAC	2 cut(s)	186, 591
Bbv12I	GWGCWC	1 cut(s)	864
BccI	CCATC	3 cut(s)	124, 274, 319
BceAI	ACGGC	2 cut(s)	213, 277
BciT130I	CCWGG	1 cut(s)	741
BclI	TGATCA	1 cut(s)	133
BcnI	CCSGG	3 cut(s)	272, 273, 702
BcoDI	GTCTC	2 cut(s)	886, 1071
BfoI	RGCGCY	1 cut(s)	405
BfrI	CTTAAG	1 cut(s)	832
BisI	GCNGC	1 cut(s)	198
BlpI	GCTNAGC	1 cut(s)	206
BlsI	GCNGC	1 cut(s)	199
Bme1390I	CCNGG	4 cut(s)	272, 273, 702, 741
Bme18I	GGWCC	1 cut(s)	697
BmeT110I	CYCGRG	2 cut(s)	271, 1035
BmgT120I	GGNCC	2 cut(s)	697, 1031
BmiI	GGNNCC	2 cut(s)	403, 699
BmrFI	CCNGG	4 cut(s)	272, 273, 702, 741
BmrI	ACTGGG	1 cut(s)	143
BmuI	ACTGGG	1 cut(s)	143
BpiI	GAAGAC	2 cut(s)	186, 591
BplI	GAGNNNNNCTC	2 cut(s)	881, 913
BpmI	CTGGAG	1 cut(s)	436
Bpu1102I	GCTNAGC	1 cut(s)	206
BpuEI	CTTGAG	1 cut(s)	1032
BpuMI	CCSGG	3 cut(s)	272, 273, 702
Bsa29I	ATCGAT	1 cut(s)	76
BsaHI	GRCGYC	1 cut(s)	402
BsaJI	CCNNGG	7 cut(s)	219, 259, 271, 571, 676, 700, 739
BsaWI	WCCGGW	2 cut(s)	753, 943
Bsc4I	CCNNNNNNNGG	2 cut(s)	235, 491
Bse118I	RCCGGY	1 cut(s)	943
Bse1I	ACTGG	2 cut(s)	149, 419
BseAI	TCCGGA	1 cut(s)	753
BseBI	CCWGG	1 cut(s)	741
BseCI	ATCGAT	1 cut(s)	76
BseDI	CCNNGG	7 cut(s)	219, 259, 271, 571, 676, 700, 739
BseGI	GGATG	2 cut(s)	119, 144
BseLI	CCNNNNNNNGG	2 cut(s)	235, 491
BseNI	ACTGG	2 cut(s)	149, 419
BseSI	GKGCMC	1 cut(s)	659
BseYI	CCCAGC	2 cut(s)	660, 880
BsgI	GTGCAG	1 cut(s)	960
Bsh1285I	CGRYCG	1 cut(s)	187
BshFI	GGCC	7 cut(s)	22, 224, 475, 612, 681, 905, 1032
BshNI	GGYRCC	1 cut(s)	401
BshTI	ACCGGT	1 cut(s)	943
BshVI	ATCGAT	1 cut(s)	76
BsiEI	CGRYCG	1 cut(s)	187
BsiHKAI	GWGCWC	1 cut(s)	864
BsiHKCI	CYCGRG	2 cut(s)	271, 1035
BsiSI	CCGG	6 cut(s)	174, 272, 702, 754, 944, 965
BslFI	GGGAC	3 cut(s)	53, 288, 722
BslI	CCNNNNNNNGG	2 cut(s)	235, 491
BsmAI	GTCTC	2 cut(s)	886, 1071
BsmFI	GGGAC	3 cut(s)	53, 288, 722
BsnI	GGCC	7 cut(s)	22, 224, 475, 612, 681, 905, 1032
BsoBI	CYCGRG	2 cut(s)	271, 1035
Bsp1286I	GDGCHC	2 cut(s)	659, 864
Bsp13I	TCCGGA	1 cut(s)	753
Bsp143I	GATC	5 cut(s)	73, 133, 184, 771, 817
Bsp1720I	GCTNAGC	1 cut(s)	206
BspACI	CCGC	3 cut(s)	198, 304, 705
BspANI	GGCC	7 cut(s)	22, 224, 475, 612, 681, 905, 1032
BspDI	ATCGAT	1 cut(s)	76
BspEI	TCCGGA	1 cut(s)	753
BspLI	GGNNCC	2 cut(s)	403, 699
BspPI	GGATC	2 cut(s)	779, 825
BspQI	GCTCTTC	1 cut(s)	917
BspT107I	GGYRCC	1 cut(s)	401
BspTI	CTTAAG	1 cut(s)	832
BsrFI	RCCGGY	1 cut(s)	943
BsrI	ACTGG	2 cut(s)	149, 419
BssAI	RCCGGY	1 cut(s)	943
BssECI	CCNNGG	7 cut(s)	219, 259, 271, 571, 676, 700, 739
BssMI	GATC	5 cut(s)	73, 133, 184, 771, 817
BssNI	GRCGYC	1 cut(s)	402
BssT1I	CCWWGG	2 cut(s)	571, 676
Bst2UI	CCWGG	1 cut(s)	741
Bst4CI	ACNGT	1 cut(s)	778
Bst6I	CTCTTC	1 cut(s)	917
BstACI	GRCGYC	1 cut(s)	402
BstAFI	CTTAAG	1 cut(s)	832
BstC8I	GCNNGC	7 cut(s)	191, 195, 226, 383, 477, 723, 975
BstDEI	CTNAG	2 cut(s)	206, 632
BstDSI	CCRYGG	1 cut(s)	259
BstF5I	GGATG	2 cut(s)	119, 144
BstH2I	RGCGCY	1 cut(s)	405
BstHHI	GCGC	1 cut(s)	404
BstKTI	GATC	5 cut(s)	76, 136, 187, 774, 820
BstMAI	GTCTC	2 cut(s)	886, 1071
BstMBI	GATC	5 cut(s)	73, 133, 184, 771, 817
BstMCI	CGRYCG	1 cut(s)	187
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 794, 961, 970
BstNI	CCWGG	1 cut(s)	741
BstNSI	RCATGY	1 cut(s)	630
BstSCI	CCNGG	4 cut(s)	270, 271, 700, 739
BstSLI	GKGCMC	1 cut(s)	659
BstV2I	GAAGAC	2 cut(s)	186, 591
BstXI	CCANNNNNNTGG	2 cut(s)	18, 620
Bsu15I	ATCGAT	1 cut(s)	76
BsuRI	GGCC	7 cut(s)	22, 224, 475, 612, 681, 905, 1032
BsuTUI	ATCGAT	1 cut(s)	76
BtgI	CCRYGG	1 cut(s)	259
BtsCI	GGATG	2 cut(s)	119, 144
BtsIMutI	CAGTG	1 cut(s)	774
Cac8I	GCNNGC	7 cut(s)	191, 195, 226, 383, 477, 723, 975
CfoI	GCGC	1 cut(s)	404
Cfr10I	RCCGGY	1 cut(s)	943
Cfr13I	GGNCC	2 cut(s)	697, 1031
Cfr9I	CCCGGG	1 cut(s)	271
ClaI	ATCGAT	1 cut(s)	76
CseI	GACGC	1 cut(s)	773
Csp6I	GTAC	2 cut(s)	41, 847
CspAI	ACCGGT	1 cut(s)	943
CviAII	CATG	2 cut(s)	137, 627
CviQI	GTAC	2 cut(s)	41, 847
DdeI	CTNAG	2 cut(s)	206, 632
DinI	GGCGCC	1 cut(s)	403
DpnI	GATC	5 cut(s)	75, 135, 186, 773, 819
DpnII	GATC	5 cut(s)	73, 133, 184, 771, 817
DraI	TTTAAA	1 cut(s)	1062
DrdI	GACNNNNNNGTC	1 cut(s)	734
DseDI	GACNNNNNNGTC	1 cut(s)	734
EaeI	YGGCCR	2 cut(s)	20, 610
Eam1104I	CTCTTC	1 cut(s)	917
EarI	CTCTTC	1 cut(s)	917
EciI	GGCGGA	1 cut(s)	720
Eco130I	CCWWGG	2 cut(s)	571, 676
Eco147I	AGGCCT	1 cut(s)	224
Eco47I	GGWCC	1 cut(s)	697
Eco88I	CYCGRG	2 cut(s)	271, 1035
EcoRI	GAATTC	1 cut(s)	308
EcoRII	CCWGG	1 cut(s)	739
EcoT14I	CCWWGG	2 cut(s)	571, 676
EcoT22I	ATGCAT	1 cut(s)	604
EgeI	GGCGCC	1 cut(s)	403
EheI	GGCGCC	1 cut(s)	403
ErhI	CCWWGG	2 cut(s)	571, 676
FaeI	CATG	2 cut(s)	140, 630
FalI	AAGNNNNNCTT	2 cut(s)	576, 608
FaqI	GGGAC	3 cut(s)	53, 288, 722
FatI	CATG	2 cut(s)	136, 626
FauI	CCCGC	1 cut(s)	297
FbaI	TGATCA	1 cut(s)	133
FblI	GTMKAC	1 cut(s)	745
Fnu4HI	GCNGC	1 cut(s)	198
FokI	GGATG	2 cut(s)	106, 131
Fsp4HI	GCNGC	1 cut(s)	198
GlaI	GCGC	1 cut(s)	403
GluI	GCNGC	1 cut(s)	198
GsaI	CCCAGC	2 cut(s)	664, 884
GsuI	CTGGAG	1 cut(s)	436
HaeII	RGCGCY	1 cut(s)	405
HaeIII	GGCC	7 cut(s)	22, 224, 475, 612, 681, 905, 1032
HapII	CCGG	6 cut(s)	174, 272, 702, 754, 944, 965
HgaI	GACGC	1 cut(s)	773
HhaI	GCGC	1 cut(s)	404
Hin1I	GRCGYC	1 cut(s)	402
Hin1II	CATG	2 cut(s)	140, 630
Hin6I	GCGC	1 cut(s)	402
HinP1I	GCGC	1 cut(s)	402
HindIII	AAGCTT	1 cut(s)	533
HinfI	GANTC	3 cut(s)	240, 894, 1055
HpaII	CCGG	6 cut(s)	174, 272, 702, 754, 944, 965
HphI	GGTGA	3 cut(s)	265, 345, 958
Hpy166II	GTNNAC	3 cut(s)	256, 746, 877
Hpy188I	TCNGA	6 cut(s)	38, 124, 133, 220, 697, 921
Hpy188III	TCNNGA	2 cut(s)	410, 754
Hpy8I	GTNNAC	3 cut(s)	256, 746, 877
Hpy99I	CGWCG	2 cut(s)	451, 691
HpyAV	CCTTC	6 cut(s)	362, 443, 489, 547, 916, 943
HpyCH4III	ACNGT	1 cut(s)	778
HpyCH4IV	ACGT	2 cut(s)	845, 995
HpyCH4V	TGCA	3 cut(s)	602, 842, 977
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 794, 961, 970
HpyF3I	CTNAG	2 cut(s)	206, 632
HpySE526I	ACGT	2 cut(s)	845, 995
Hsp92I	GRCGYC	1 cut(s)	402
Hsp92II	CATG	2 cut(s)	140, 630
HspAI	GCGC	1 cut(s)	402
KasI	GGCGCC	1 cut(s)	401
Kpn2I	TCCGGA	1 cut(s)	753
Ksp22I	TGATCA	1 cut(s)	133
Kzo9I	GATC	5 cut(s)	73, 133, 184, 771, 817
LguI	GCTCTTC	1 cut(s)	917
LmnI	GCTCC	4 cut(s)	378, 417, 793, 952
MaeII	ACGT	2 cut(s)	845, 995
MaeIII	GTNAC	2 cut(s)	561, 958
MalI	GATC	5 cut(s)	75, 135, 186, 773, 819
MboI	GATC	5 cut(s)	73, 133, 184, 771, 817
MboII	GAAGA	8 cut(s)	191, 235, 304, 434, 584, 596, 901, 934
MhlI	GDGCHC	2 cut(s)	659, 864
MlsI	TGGCCA	2 cut(s)	22, 612
MluCI	AATT	3 cut(s)	308, 390, 822
MluNI	TGGCCA	2 cut(s)	22, 612
Mly113I	GGCGCC	1 cut(s)	402
MlyI	GAGTC	1 cut(s)	888
MmeI	TCCRAC	1 cut(s)	78
MnlI	CCTC	7 cut(s)	214, 392, 491, 517, 537, 1031, 1044
Mox20I	TGGCCA	2 cut(s)	22, 612
Mph1103I	ATGCAT	1 cut(s)	604
MroI	TCCGGA	1 cut(s)	753
MroXI	GAANNNNTTC	1 cut(s)	588
MscI	TGGCCA	2 cut(s)	22, 612
MseI	TTAA	3 cut(s)	455, 833, 1061
Msp20I	TGGCCA	2 cut(s)	22, 612
MspCI	CTTAAG	1 cut(s)	832
MspI	CCGG	6 cut(s)	174, 272, 702, 754, 944, 965
MspR9I	CCNGG	4 cut(s)	272, 273, 702, 741
MvaI	CCWGG	1 cut(s)	741
MwoI	GCNNNNNNNGC	4 cut(s)	174, 794, 961, 970
NarI	GGCGCC	1 cut(s)	402
NciI	CCSGG	3 cut(s)	272, 273, 702
NdeII	GATC	5 cut(s)	73, 133, 184, 771, 817
NlaIII	CATG	2 cut(s)	140, 630
NlaIV	GGNNCC	2 cut(s)	403, 699
NmuCI	GTSAC	1 cut(s)	561
NsiI	ATGCAT	1 cut(s)	604
NspI	RCATGY	1 cut(s)	630
PaeR7I	CTCGAG	1 cut(s)	1035
PceI	AGGCCT	1 cut(s)	224
PciSI	GCTCTTC	1 cut(s)	917
PdmI	GAANNNNTTC	1 cut(s)	588
PfeI	GAWTC	2 cut(s)	240, 1055
PinAI	ACCGGT	1 cut(s)	943
PkrI	GCNGC	1 cut(s)	199
Ple19I	CGATCG	1 cut(s)	187
PleI	GAGTC	1 cut(s)	888
PluTI	GGCGCC	1 cut(s)	405
PpsI	GAGTC	1 cut(s)	888
Psp6I	CCWGG	1 cut(s)	739
PspFI	CCCAGC	2 cut(s)	660, 880
PspGI	CCWGG	1 cut(s)	739
PspN4I	GGNNCC	2 cut(s)	403, 699
PspPI	GGNCC	2 cut(s)	697, 1031
PspXI	VCTCGAGB	1 cut(s)	1035
PvuI	CGATCG	1 cut(s)	187
RsaI	GTAC	2 cut(s)	42, 848
RsaNI	GTAC	2 cut(s)	41, 847
SapI	GCTCTTC	1 cut(s)	917
SaqAI	TTAA	3 cut(s)	455, 833, 1061
SatI	GCNGC	1 cut(s)	198
Sau3AI	GATC	5 cut(s)	73, 133, 184, 771, 817
Sau96I	GGNCC	2 cut(s)	697, 1031
SchI	GAGTC	1 cut(s)	888
ScrFI	CCNGG	4 cut(s)	272, 273, 702, 741
SduI	GDGCHC	2 cut(s)	659, 864
SfoI	GGCGCC	1 cut(s)	403
Sfr274I	CTCGAG	1 cut(s)	1035
SinI	GGWCC	1 cut(s)	697
SlaI	CTCGAG	1 cut(s)	1035
SmaI	CCCGGG	1 cut(s)	273
SmlI	CTYRAG	3 cut(s)	832, 1035, 1047
SmoI	CTYRAG	3 cut(s)	832, 1035, 1047
Sse9I	AATT	3 cut(s)	308, 390, 822
SseBI	AGGCCT	1 cut(s)	224
SsiI	CCGC	3 cut(s)	198, 304, 705
SspDI	GGCGCC	1 cut(s)	401
SspI	AATATT	2 cut(s)	811, 983
StuI	AGGCCT	1 cut(s)	224
StyD4I	CCNGG	4 cut(s)	270, 271, 700, 739
StyI	CCWWGG	2 cut(s)	571, 676
TaaI	ACNGT	1 cut(s)	778
TaiI	ACGT	2 cut(s)	848, 998
TaqI	TCGA	3 cut(s)	76, 1036, 1053
TasI	AATT	3 cut(s)	308, 390, 822
TatI	WGTACW	1 cut(s)	40
TauI	GCSGC	1 cut(s)	200
TfiI	GAWTC	2 cut(s)	240, 1055
Tru1I	TTAA	3 cut(s)	455, 833, 1061
Tru9I	TTAA	3 cut(s)	455, 833, 1061
TscAI	CASTG	1 cut(s)	781
TseFI	GTSAC	1 cut(s)	561
Tsp45I	GTSAC	1 cut(s)	561
TspDTI	ATGAA	2 cut(s)	381, 1017
TspGWI	ACGGA	1 cut(s)	435
TspMI	CCCGGG	1 cut(s)	271
TspRI	CASTG	1 cut(s)	781
Vha464I	CTTAAG	1 cut(s)	832
VpaK11BI	GGWCC	1 cut(s)	697
XapI	RAATTY	2 cut(s)	308, 822
XceI	RCATGY	1 cut(s)	630
XhoI	CTCGAG	1 cut(s)	1035
XmaI	CCCGGG	1 cut(s)	271
XmiI	GTMKAC	1 cut(s)	745
XmnI	GAANNNNTTC	1 cut(s)	588
Zsp2I	ATGCAT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.