Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0017717

Physical Location & Seq

Genomic Coordinates Forward (+)

26381586 .. 26383658

Gene Structure 2073 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1156100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2073 bp

ATGGAAGCAGCTCTTGTTCCTCCTCTAATGGTTTCCACAGGATTTTTGCCAACTGGGTCGTTGTTACAGTCGTTGGTTCACATATGCAACGAAGTTTGCTCCATGGAAAAGCTTCCGGTTCTACAGGGCCGGAACATCTCCACCATGATAAGGAGGATCAAGCTTCTCGCTTCTCTGTTCGAAGAGATACAAGAGTCCGACGGGCCGCTTCCTCCCTCTTCAATCCTATGCCTCACGGAGCTTTTCTCCGTGATTCGAAGAGCCAAGTTTCTGATCCAAGATTGTAAAGACGGGAGCTCTCTGTGGGGGCTTATGCAGACGGAGTTGGTTTCGAATCAGTTTTACGTGCTGGTCAAGGAGTTGGGGAGGGCGCATGATATTTTGCCTCTGAGTTTGCTTAACGTGACGGCAGATATCAAAGAGCAGATCGAGCTCCTCCACCGGCAAGCGAAAAGAACGGAGCTTTTTATCGATCTCAAAGAGCTGCAGAGAAGAGAAGAGCTTTTGGTTTTGATGGGAAGCAATAGTGAGAAGAACAGGAAGAACAAAGGGTTCATAGATTTGGTCAAAGTGAGAGATGTTCTGAGCAGCATTGGCCTGAGAAACGCCGTGGATTACGAAGAAGAAACTTCAAAGCTCGAGGCAGAAGCTCACAAGCAAGCAGGGAGTGGAGGGCTGATTGTGGTGTCCAACATAAACAATCTTATTTCCCTTGTGTCGTACTGCAAAACCATCATTTTTATAGATGGTGAAATCGAAAAACCGTGTAAAGAGGATATGAAACTGCACTGTCTCCCCTCGGGTAGATTTTATGATCACTCTTCATCTTCTCAGTCGTTGATCCCCAATGTTCCGGATGAATTTCGCTGCCCGATTTCATTGGACTTGATTAGAGACCCTGTTATTGTAGCATCCGGGCACACTTATGATCGAAATTCGATTGCACAATGGATAAATTCGGGGCACCAGACATGTCCCAAAAGCGGGCAGAAGCTGATTCACATGGCGCTTATTCCCAACTACGCACTCAGGAGTCTAATGCAACAATGGTGTGAGGAGAACAACGTTCCCACAACTGAATCCTCGCAGTCTTCTTCCTCCGATTTGGCGAGGAGCAGCAGCAAAAGGGAGTTGTACGAAAATGCTGTCGATCACATTTCTGTCGTGAAAGCTGCTGCTGATGCTGTGAAATTGACAGCTGAGTTTCTGGTGGGAAAACTAGCAACTGGCTCCCCAGATATCCAAAGGCAAGCGGCCTATGAGCTCCGATTACTTGCCAAAACCGGCATGGATAATAGAAGGATAATAGCAGAGGCCGGAGCTATTCCATTTCTAGTGACGTTGCTGAGATCCCATGAACCGAGAATTCAGGAAAACGCTGTCACAGCGTTGCTCAACCTCTCCATCTACAACAACAACAAGATTCTGATCATGGCGGCTGGAGCAATTGACGAAATAGTAAACGTCTTGGAATCGGGGAACACAATGGAAGCAAGAGAGAATGCAGCAGCAGCAATTTTCAGCTTGTCGATGATAGATGACTGCAAGATAACGATTGGAAAGCGCCCTAGAGCCATTCCGGCATTGGTGGGGCTTTTGAAAGAAGGCACTCCGGCTGGTAAAAAAGATGCTGCCGTAGCACTCTTCAATCTTGCACTCTACAATGCCAACAAGGTGAGCATTGTGTTCGCCGGGGCGGTTCCTCTGCTCATCGAGCTGTTGATGGACGACAAGGCGGGAATTACAGATGATGCCTTGGCACTGCTTGCTCAGATTTTGGGTTGCTCTGAAGGACTGCAGGAGATTGGGAAGAGCAGGATATTAGTGCCTATTCTTATCGATCTCTTAAGATTTGGTTCACCGAAAGGGAAGGAAAACGCGATCACTCTTCTGTTGGGGCTGTGCAAAGATGGAGGAGAGGAGGTCGCAAGACGGCTGTTGATGAACCCGCGGAGCATTCCTTCACTCCAAAGCTTGGCTGCAGACGGTTCCTTGAAAGCTCGAAGAAAAGCTGACGCAGTGCTTCGATTACTGAACAGGTGCTGCTTCCAATCTCACGACCAGATTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002237 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009608 GO:0009609 GO:0009610 GO:0009617 GO:0009620 GO:0009877 GO:0009987 GO:0010033 GO:0010941 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0033612 GO:0036211 GO:0036377 GO:0042221 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0060548 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

691

Amino Acids

75.62

Weight (kDa)

6.5

Isoelectric Point (pI)

50.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCAfunc	PF19584	43 - 151	2.3e-06	MCAfunc domain
PUB10_N	PF25368	46 - 153	3.4e-14	PUB10-like, N-terminal domain
U-box	PF04564	284 - 355	1e-20	U-box domain
ARM_PUB	PF25598	416 - 680	4.1e-21	Plant U-box protein ARM repeat region
Arm	PF00514	431 - 468	9.8e-10	Armadillo/beta-catenin-like repeat
ARM_ARMC5	PF24768	431 - 598	1.3e-09	ARMC5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0017717)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g24820
malus_domestica	MD01G1156100.v1.1 MD07G1224800.v1.1
prunus_persica	Prupe.2G253400_v2.0.a1
pyrus_communis	pycom01g17300
rosa_chinensis	RchiOBHm_Chr1g0370691
rosa_laevigata	RLG00000027017
rosa_multiflora	Rmu_sc0004550.1_g000011
rosa_roxburghii	Rroxscaffold_4G00286300
rosa_rugosa	Rorug01G0358500
rosa_samantha	Rh1DG363200
rosa_wichuraiana	Rw1G032400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	963
AccB7I	CCANNNNNTGG	1 cut(s)	1975
AccII	CGCG	2 cut(s)	1880, 1951
AccIII	TCCGGA	1 cut(s)	853
AciI	CCGC	8 cut(s)	206, 984, 1253, 1436, 1697, 1736, 1949, 1951
AclI	AACGTT	1 cut(s)	1065
AclWI	GGATC	4 cut(s)	164, 268, 835, 1344
AcsI	RAATTY	4 cut(s)	860, 934, 955, 1365
AcuI	CTGAAG	1 cut(s)	1809
AfaI	GTAC	2 cut(s)	722, 1136
AfiI	CCNNNNNNNGG	4 cut(s)	150, 983, 984, 1975
AflII	CTTAAG	1 cut(s)	1846
AflIII	ACRYGT	1 cut(s)	971
AgsI	TTSAA	5 cut(s)	222, 633, 1600, 1648, 1996
Alw21I	GWGCWC	3 cut(s)	299, 435, 1266
Alw26I	GTCTC	2 cut(s)	797, 888
AlwI	GGATC	4 cut(s)	164, 268, 835, 1344
AlwNI	CAGNNNCTG	2 cut(s)	994, 2043
Ama87I	CYCGRG	2 cut(s)	638, 799
Aor13HI	TCCGGA	1 cut(s)	853
AoxI	GGCC	5 cut(s)	127, 203, 595, 1254, 1314
ApoI	RAATTY	4 cut(s)	860, 934, 955, 1365
ArsI	GACNNNNNNTTYG	2 cut(s)	1131, 1163
Asp700I	GAANNNNTTC	1 cut(s)	111
AspLEI	GCGC	3 cut(s)	373, 1009, 1566
AspS9I	GGNCC	2 cut(s)	127, 203
AsuC2I	CCSGG	2 cut(s)	916, 1693
AsuHPI	GGTGA	3 cut(s)	761, 1687, 1851
AsuII	TTCGAA	3 cut(s)	180, 256, 332
AvaI	CYCGRG	2 cut(s)	638, 799
BaeGI	GKGCMC	2 cut(s)	921, 966
BaeI	ACNNNNGTAYC	2 cut(s)	179, 212
BanI	GGYRCC	1 cut(s)	963
BanII	GRGCYC	3 cut(s)	299, 435, 1266
BbsI	GAAGAC	1 cut(s)	1083
Bbv12I	GWGCWC	3 cut(s)	299, 435, 1266
BccI	CCATC	6 cut(s)	508, 740, 740, 1412, 1717, 1904
BceAI	ACGGC	4 cut(s)	423, 593, 1619, 1949
BcgI	CGANNNNNNTGC	2 cut(s)	1669, 1703
BclI	TGATCA	2 cut(s)	814, 1428
BcnI	CCSGG	2 cut(s)	916, 1693
BcoDI	GTCTC	2 cut(s)	797, 888
BfaI	CTAG	3 cut(s)	1220, 1334, 1569
BfmI	CTRYAG	4 cut(s)	122, 485, 1796, 1980
BfoI	RGCGCY	2 cut(s)	1010, 1567
BfrI	CTTAAG	1 cut(s)	1846
BglI	GCCNNNNNGGC	1 cut(s)	1580
Bme1390I	CCNGG	2 cut(s)	916, 1693
BmeT110I	CYCGRG	2 cut(s)	638, 799
BmgT120I	GGNCC	2 cut(s)	127, 203
BmiI	GGNNCC	4 cut(s)	965, 1231, 1701, 1990
BmrFI	CCNGG	2 cut(s)	916, 1693
BmrI	ACTGGG	1 cut(s)	63
BmsI	GCATC	4 cut(s)	920, 1171, 1618, 1741
BmuI	ACTGGG	1 cut(s)	63
BpiI	GAAGAC	1 cut(s)	1083
BplI	GAGNNNNNCTC	2 cut(s)	230, 262
BpmI	CTGGAG	1 cut(s)	1461
Bpu14I	TTCGAA	3 cut(s)	180, 256, 332
BpuMI	CCSGG	2 cut(s)	916, 1693
Bsa29I	ATCGAT	2 cut(s)	471, 1839
BsaAI	YACGTR	1 cut(s)	346
BsaBI	GATNNNNATC	1 cut(s)	1427
BsaI	GGTCTC	1 cut(s)	888
BsaJI	CCNNGG	6 cut(s)	102, 609, 798, 1692, 1755, 1949
BsaWI	WCCGGW	2 cut(s)	115, 853
BsaXI	ACNNNNNCTCC	2 cut(s)	1908, 1938
Bsc4I	CCNNNNNNNGG	4 cut(s)	150, 983, 984, 1975
Bse118I	RCCGGY	2 cut(s)	441, 1283
Bse1I	ACTGG	2 cut(s)	58, 1231
Bse8I	GATNNNNATC	1 cut(s)	1427
BseAI	TCCGGA	1 cut(s)	853
BseCI	ATCGAT	2 cut(s)	471, 1839
BseDI	CCNNGG	6 cut(s)	102, 609, 798, 1692, 1755, 1949
BseGI	GGATG	2 cut(s)	862, 911
BseJI	GATNNNNATC	1 cut(s)	1427
BseLI	CCNNNNNNNGG	4 cut(s)	150, 983, 984, 1975
BseMII	CTCAG	8 cut(s)	380, 575, 590, 845, 1042, 1191, 1337, 1784
BseNI	ACTGG	2 cut(s)	58, 1231
BseRI	GAGGAG	6 cut(s)	12, 425, 1070, 1126, 1929, 1934
BseSI	GKGCMC	2 cut(s)	921, 966
BsgI	GTGCAG	1 cut(s)	770
Bsh1236I	CGCG	2 cut(s)	1880, 1951
BshFI	GGCC	5 cut(s)	129, 205, 597, 1256, 1316
BshNI	GGYRCC	1 cut(s)	963
BshVI	ATCGAT	2 cut(s)	471, 1839
BsiHKAI	GWGCWC	3 cut(s)	299, 435, 1266
BsiHKCI	CYCGRG	2 cut(s)	638, 799
BslFI	GGGAC	1 cut(s)	960
BslI	CCNNNNNNNGG	4 cut(s)	150, 983, 984, 1975
BsmAI	GTCTC	2 cut(s)	797, 888
BsmFI	GGGAC	1 cut(s)	960
BsmI	GAATGC	2 cut(s)	1507, 1956
BsnI	GGCC	5 cut(s)	129, 205, 597, 1256, 1316
Bso31I	GGTCTC	1 cut(s)	888
BsoBI	CYCGRG	2 cut(s)	638, 799
Bsp119I	TTCGAA	3 cut(s)	180, 256, 332
Bsp1286I	GDGCHC	5 cut(s)	299, 435, 921, 966, 1266
Bsp13I	TCCGGA	1 cut(s)	853
Bsp19I	CCATGG	1 cut(s)	102
BspACI	CCGC	8 cut(s)	206, 984, 1253, 1436, 1697, 1736, 1949, 1951
BspANI	GGCC	5 cut(s)	129, 205, 597, 1256, 1316
BspCNI	CTCAG	8 cut(s)	381, 576, 591, 844, 1041, 1192, 1338, 1783
BspDI	ATCGAT	2 cut(s)	471, 1839
BspEI	TCCGGA	1 cut(s)	853
BspFNI	CGCG	2 cut(s)	1880, 1951
BspLI	GGNNCC	4 cut(s)	965, 1231, 1701, 1990
BspMAI	CTGCAG	3 cut(s)	489, 1800, 1984
BspPI	GGATC	4 cut(s)	164, 268, 835, 1344
BspQI	GCTCTTC	3 cut(s)	253, 492, 1805
BspT104I	TTCGAA	3 cut(s)	180, 256, 332
BspT107I	GGYRCC	1 cut(s)	963
BspTI	CTTAAG	1 cut(s)	1846
BspTNI	GGTCTC	1 cut(s)	888
BsrFI	RCCGGY	2 cut(s)	441, 1283
BsrI	ACTGG	2 cut(s)	58, 1231
BssAI	RCCGGY	2 cut(s)	441, 1283
BssECI	CCNNGG	6 cut(s)	102, 609, 798, 1692, 1755, 1949
BssT1I	CCWWGG	2 cut(s)	102, 1755
Bst4CI	ACNGT	4 cut(s)	69, 765, 791, 1988
Bst6I	CTCTTC	9 cut(s)	177, 223, 253, 487, 492, 826, 1649, 1805, 1893
BstAFI	CTTAAG	1 cut(s)	1846
BstAPI	GCANNNNNTGC	1 cut(s)	1766
BstBAI	YACGTR	1 cut(s)	346
BstBI	TTCGAA	3 cut(s)	180, 256, 332
BstC8I	GCNNGC	5 cut(s)	447, 660, 986, 1251, 1767
BstDEI	CTNAG	8 cut(s)	389, 584, 599, 831, 1028, 1200, 1346, 1770
BstDSI	CCRYGG	3 cut(s)	102, 609, 1949
BstF5I	GGATG	2 cut(s)	862, 911
BstFNI	CGCG	2 cut(s)	1880, 1951
BstH2I	RGCGCY	2 cut(s)	1010, 1567
BstHHI	GCGC	3 cut(s)	373, 1009, 1566
BstMAI	GTCTC	2 cut(s)	797, 888
BstNSI	RCATGY	1 cut(s)	975
BstSCI	CCNGG	2 cut(s)	914, 1691
BstSFI	CTRYAG	4 cut(s)	122, 485, 1796, 1980
BstSLI	GKGCMC	2 cut(s)	921, 966
BstUI	CGCG	2 cut(s)	1880, 1951
BstV2I	GAAGAC	1 cut(s)	1083
BstX2I	RGATCY	1 cut(s)	1349
BstYI	RGATCY	1 cut(s)	1349
Bsu15I	ATCGAT	2 cut(s)	471, 1839
BsuRI	GGCC	5 cut(s)	129, 205, 597, 1256, 1316
BsuTUI	ATCGAT	2 cut(s)	471, 1839
BtgI	CCRYGG	3 cut(s)	102, 609, 1949
BtsCI	GGATG	2 cut(s)	862, 911
BtsI	GCAGTG	2 cut(s)	1760, 2025
BtsIMutI	CAGTG	3 cut(s)	787, 1760, 2025
Cac8I	GCNNGC	5 cut(s)	447, 660, 986, 1251, 1767
CaiI	CAGNNNCTG	2 cut(s)	994, 2043
CfoI	GCGC	3 cut(s)	373, 1009, 1566
Cfr10I	RCCGGY	2 cut(s)	441, 1283
Cfr13I	GGNCC	2 cut(s)	127, 203
Cfr42I	CCGCGG	1 cut(s)	1952
ClaI	ATCGAT	2 cut(s)	471, 1839
CseI	GACGC	1 cut(s)	2024
Csp6I	GTAC	2 cut(s)	721, 1135
CviAII	CATG	8 cut(s)	103, 145, 374, 972, 1003, 1288, 1355, 1432
CviQI	GTAC	2 cut(s)	721, 1135
DdeI	CTNAG	8 cut(s)	389, 584, 599, 831, 1028, 1200, 1346, 1770
Eam1104I	CTCTTC	9 cut(s)	177, 223, 253, 487, 492, 826, 1649, 1805, 1893
EarI	CTCTTC	9 cut(s)	177, 223, 253, 487, 492, 826, 1649, 1805, 1893
Ecl136II	GAGCTC	3 cut(s)	297, 433, 1264
Eco130I	CCWWGG	2 cut(s)	102, 1755
Eco24I	GRGCYC	3 cut(s)	299, 435, 1266
Eco31I	GGTCTC	1 cut(s)	888
Eco32I	GATATC	2 cut(s)	415, 1240
Eco53kI	GAGCTC	3 cut(s)	297, 433, 1264
Eco57I	CTGAAG	1 cut(s)	1809
Eco88I	CYCGRG	2 cut(s)	638, 799
EcoICRI	GAGCTC	3 cut(s)	297, 433, 1264
EcoRI	GAATTC	1 cut(s)	1365
EcoRV	GATATC	2 cut(s)	415, 1240
EcoT14I	CCWWGG	2 cut(s)	102, 1755
EcoT38I	GRGCYC	3 cut(s)	299, 435, 1266
ErhI	CCWWGG	2 cut(s)	102, 1755
FaeI	CATG	8 cut(s)	106, 148, 377, 975, 1006, 1291, 1358, 1435
FalI	AAGNNNNNCTT	1 cut(s)	29
FaqI	GGGAC	1 cut(s)	960
FatI	CATG	8 cut(s)	102, 144, 373, 971, 1002, 1287, 1354, 1431
FauI	CCCGC	3 cut(s)	977, 1729, 1956
FauNDI	CATATG	1 cut(s)	83
FbaI	TGATCA	2 cut(s)	814, 1428
FokI	GGATG	2 cut(s)	869, 898
FriOI	GRGCYC	3 cut(s)	299, 435, 1266
FspBI	CTAG	3 cut(s)	1220, 1334, 1569
GlaI	GCGC	3 cut(s)	372, 1008, 1565
GsuI	CTGGAG	1 cut(s)	1461
HaeII	RGCGCY	2 cut(s)	1010, 1567
HaeIII	GGCC	5 cut(s)	129, 205, 597, 1256, 1316
HgaI	GACGC	1 cut(s)	2024
HhaI	GCGC	3 cut(s)	373, 1009, 1566
Hin1II	CATG	8 cut(s)	106, 148, 377, 975, 1006, 1291, 1358, 1435
Hin6I	GCGC	3 cut(s)	371, 1007, 1564
HinP1I	GCGC	3 cut(s)	371, 1007, 1564
HindIII	AAGCTT	3 cut(s)	110, 161, 1972
HinfI	GANTC	8 cut(s)	194, 253, 334, 997, 1033, 1079, 1423, 1472
HphI	GGTGA	3 cut(s)	761, 1687, 1851
Hpy166II	GTNNAC	3 cut(s)	79, 1462, 1859
Hpy188I	TCNGA	9 cut(s)	199, 273, 390, 585, 1102, 1268, 1428, 1773, 1789
Hpy188III	TCNNGA	5 cut(s)	854, 1030, 1165, 1370, 2057
Hpy8I	GTNNAC	3 cut(s)	79, 1462, 1859
Hpy99I	CGWCG	1 cut(s)	203
HpyAV	CCTTC	5 cut(s)	1293, 1598, 1784, 1864, 1971
HpyCH4III	ACNGT	4 cut(s)	69, 765, 791, 1988
HpyCH4IV	ACGT	5 cut(s)	345, 402, 1065, 1340, 1464
HpyF3I	CTNAG	8 cut(s)	389, 584, 599, 831, 1028, 1200, 1346, 1770
HpySE526I	ACGT	5 cut(s)	345, 402, 1065, 1340, 1464
Hsp92II	CATG	8 cut(s)	106, 148, 377, 975, 1006, 1291, 1358, 1435
HspAI	GCGC	3 cut(s)	371, 1007, 1564
Kpn2I	TCCGGA	1 cut(s)	853
Ksp22I	TGATCA	2 cut(s)	814, 1428
KspI	CCGCGG	1 cut(s)	1952
LguI	GCTCTTC	3 cut(s)	253, 492, 1805
LweI	GCATC	4 cut(s)	920, 1171, 1618, 1741
MaeI	CTAG	3 cut(s)	1220, 1334, 1569
MaeII	ACGT	5 cut(s)	345, 402, 1065, 1340, 1464
MaeIII	GTNAC	4 cut(s)	63, 403, 1336, 1381
MfeI	CAATTG	1 cut(s)	1446
MflI	RGATCY	1 cut(s)	1349
MhlI	GDGCHC	5 cut(s)	299, 435, 921, 966, 1266
MluCI	AATT	8 cut(s)	860, 934, 955, 1190, 1365, 1446, 1515, 1740
MlyI	GAGTC	2 cut(s)	203, 1042
MmeI	TCCRAC	2 cut(s)	222, 714
MroI	TCCGGA	1 cut(s)	853
MroXI	GAANNNNTTC	1 cut(s)	111
MseI	TTAA	2 cut(s)	399, 1847
MslI	CAYNNNNRTG	1 cut(s)	924
MspA1I	CMGCKG	2 cut(s)	1199, 1951
MspCI	CTTAAG	1 cut(s)	1846
MspR9I	CCNGG	2 cut(s)	916, 1693
MunI	CAATTG	1 cut(s)	1446
Mva1269I	GAATGC	2 cut(s)	1507, 1956
MvnI	CGCG	2 cut(s)	1880, 1951
NciI	CCSGG	2 cut(s)	916, 1693
NcoI	CCATGG	1 cut(s)	102
NdeI	CATATG	1 cut(s)	83
NlaIII	CATG	8 cut(s)	106, 148, 377, 975, 1006, 1291, 1358, 1435
NlaIV	GGNNCC	4 cut(s)	965, 1231, 1701, 1990
NmuCI	GTSAC	3 cut(s)	403, 1336, 1381
NspI	RCATGY	1 cut(s)	975
NspV	TTCGAA	3 cut(s)	180, 256, 332
PaeR7I	CTCGAG	1 cut(s)	638
PciI	ACATGT	1 cut(s)	971
PciSI	GCTCTTC	3 cut(s)	253, 492, 1805
PctI	GAATGC	2 cut(s)	1507, 1956
PdmI	GAANNNNTTC	1 cut(s)	111
PfeI	GAWTC	6 cut(s)	253, 334, 997, 1079, 1423, 1472
PflMI	CCANNNNNTGG	1 cut(s)	1975
PleI	GAGTC	2 cut(s)	202, 1041
PpsI	GAGTC	2 cut(s)	202, 1041
Ppu21I	YACGTR	1 cut(s)	346
PscI	ACATGT	1 cut(s)	971
Psp124BI	GAGCTC	3 cut(s)	299, 435, 1266
Psp1406I	AACGTT	1 cut(s)	1065
PspN4I	GGNNCC	4 cut(s)	965, 1231, 1701, 1990
PspPI	GGNCC	2 cut(s)	127, 203
PspXI	VCTCGAGB	1 cut(s)	638
PstI	CTGCAG	3 cut(s)	489, 1800, 1984
PstNI	CAGNNNCTG	2 cut(s)	994, 2043
PsuI	RGATCY	1 cut(s)	1349
PvuII	CAGCTG	1 cut(s)	1199
RsaI	GTAC	2 cut(s)	722, 1136
RsaNI	GTAC	2 cut(s)	721, 1135
RseI	CAYNNNNRTG	1 cut(s)	924
SacI	GAGCTC	3 cut(s)	299, 435, 1266
SacII	CCGCGG	1 cut(s)	1952
SapI	GCTCTTC	3 cut(s)	253, 492, 1805
SaqAI	TTAA	2 cut(s)	399, 1847
Sau96I	GGNCC	2 cut(s)	127, 203
SchI	GAGTC	2 cut(s)	203, 1042
ScrFI	CCNGG	2 cut(s)	916, 1693
SduI	GDGCHC	5 cut(s)	299, 435, 921, 966, 1266
SfaNI	GCATC	4 cut(s)	920, 1171, 1618, 1741
SfcI	CTRYAG	4 cut(s)	122, 485, 1796, 1980
Sfr274I	CTCGAG	1 cut(s)	638
Sfr303I	CCGCGG	1 cut(s)	1952
SfuI	TTCGAA	3 cut(s)	180, 256, 332
SgrBI	CCGCGG	1 cut(s)	1952
SlaI	CTCGAG	1 cut(s)	638
SmiMI	CAYNNNNRTG	1 cut(s)	924
SmlI	CTYRAG	2 cut(s)	638, 1846
SmoI	CTYRAG	2 cut(s)	638, 1846
Sse9I	AATT	8 cut(s)	860, 934, 955, 1190, 1365, 1446, 1515, 1740
SsiI	CCGC	8 cut(s)	206, 984, 1253, 1436, 1697, 1736, 1949, 1951
SspMI	CTAG	3 cut(s)	1220, 1334, 1569
SstI	GAGCTC	3 cut(s)	299, 435, 1266
StyD4I	CCNGG	2 cut(s)	914, 1691
StyI	CCWWGG	2 cut(s)	102, 1755
TaaI	ACNGT	4 cut(s)	69, 765, 791, 1988
TaiI	ACGT	5 cut(s)	348, 405, 1068, 1343, 1467
TasI	AATT	8 cut(s)	860, 934, 955, 1190, 1365, 1446, 1515, 1740
TauI	GCSGC	3 cut(s)	208, 1256, 1439
TfiI	GAWTC	6 cut(s)	253, 334, 997, 1079, 1423, 1472
Tru1I	TTAA	2 cut(s)	399, 1847
Tru9I	TTAA	2 cut(s)	399, 1847
TscAI	CASTG	3 cut(s)	794, 1767, 2025
TseFI	GTSAC	3 cut(s)	403, 1336, 1381
Tsp45I	GTSAC	3 cut(s)	403, 1336, 1381
TspDTI	ATGAA	7 cut(s)	544, 794, 813, 867, 873, 1371, 1958
TspGWI	ACGGA	4 cut(s)	238, 251, 335, 473
TspRI	CASTG	3 cut(s)	794, 1767, 2025
Van91I	CCANNNNNTGG	1 cut(s)	1975
Vha464I	CTTAAG	1 cut(s)	1846
XapI	RAATTY	4 cut(s)	860, 934, 955, 1365
XceI	RCATGY	1 cut(s)	975
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1285, 1582
XhoI	CTCGAG	1 cut(s)	638
XmnI	GAANNNNTTC	1 cut(s)	111
XspI	CTAG	3 cut(s)	1220, 1334, 1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.