Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0017717

Physical Location & Seq

Genomic Coordinates Forward (+)

30154498 .. 30156561

Gene Structure 2064 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1224800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2064 bp

ATGGATGTAGCTCTTGTTCCTCCTCTAATGGTTTCCACGGGATTTTTGCCGACTGGGTCGTTGTTACAATCATTGGTTCACATATGCAACGAAGTTTGCTCCATGGAAAAGCTTCCGGTTTTGCAGGGCCGGAACATCTCCACCATGATAAGGAGGATCAAGCTCCTCGCTTCTCTGTTCGAAGAGATACAAGAATCAAACGGCCCGCTTCCTCCCTCTTCGATCCTTTGCCTCACGGAGCTTTTCTCGGTGATTCGAAGAGTCAAGTTTCTGATCCAAGATTGTAAAGACGGGAGCTCTCTGTGGGGGCTTATGCAGACTGAGTTGGTTTCGAATCAGTTTTACGTGCTGGTGAAGGAATTGGGGAGGGCTCTTGATATTCTGCCACTGAGTTTGCTTAAGGTGACGGCAGATATAAGAGAGCAGGTCGAGCTTCTTCACCGGCAAGCGAAAAGAGTGGAGCTTTTTAACCATCTCAAAGAGCTGCAGAGAAGAGAAGAGCTTTTGGTTTTGATGGGAAGCAATAGTGAGAAGAACAAAGGGTTCATAGATTTGGTCAAAGTGAGAGATGTTCTGAGCAGCATTGGCCTGAGAAACACCGTGGATTACGAAGAAGAAACCTCGAAGCTTGAGGCAGAAGCTCGCAAGCAAGCAGGCAGCGGAGGGCTGATTGTGGTGTCAAACATAAACAATCTCATTTCCCTTGTTTCGTATTGCAAGACCATCATTTTTACTGATGATGAATTCGAAAAACCGAGTAAAGAGGATATGAAACTGCAGTGTGTCCGCTCGGGTAGATATTATGATCATTCTTCATCTTCTAAATCGTTGATTCTCAATGTTCCGCATGAATTTCGCTGCCCGATTTCATTGGATTTGATAAGAGACCCTGTTATTGTAGCATCCGGGCACACTTATGACCGAAATTCGATTGCACAATGGATCAATTCAGGGCACCAGACGTGCCCCAAAAGCGGGCAGAAGCTGATTCACATAGCGCTGATTCCAAACTATGCACTCAAGAGTCTAATGCAACAATGGTGCGAGGAGAACAACGTTCCCACAACTGAATCTTCCCAGTCTTCTTCCTCCGATTTGGAGAGGAGCAACAGTAAAAGGGAATTGTACGAGGACGCCGTGGATCACATTTCAGTTGTAAAAGCTGCTGTTGATGCTGTGAAATTGACAGCTGAGTTTCTGGTGGGAAAACTAGCAACTGGCTCACCGGATATCCAAAGGCAAGCGGCGTATGAGCTAAGATTACTTGCTAAAACCGGCATGGATAATAGAAGGATCATAGCTGAGGCGGGAGCTATTCCGTTTTTGGTGACATTGCTGAAATCCCATGAACCGAGAATACAGGAAAATGCAGTTACTGCACTGCTCAACCTCTCCATCTACAACAACAACAAGATTCTGATCATGGCGGCAGGAGCAATTGACAACATAGTAAACGTCTTGGAATCAGGGAACACAATGGAAGCAAGAGAGAATGCAGCAGCAGCAATTTTCAGCTTGTCCATGATAGATGACTGCAAGGTAACGATTGGAAAGCACCCTAGAGCGATTCTGGCATTGGTGGGGCTTTTGAAAGAAGGCACTCCGGCTGGTAAAAAAGATGCTGCCATAGCACTCTTCAATCTTGCATTCTACAATGCCAACAAGGTGAGCGCTGTGTTTGCTGGGGCGGTTCCTCTCCTCATTGAGCTGTTGATGGATGACAAGGCAGGCATTACGGATGATGCCTTGGCAGTGCTTGCTCAGCTTTTGGGTTGCTCTGAAGGGATGGAAGAGATTGGGAAAAGCAGGATTTTAGTGCCTATTCTTATCGATCTCTTAAGATTTGGTTCTCCAAAGGGGAAGGAAAACTCAATCACTCTTCTGCTGGGGCTGTGCAAAGATGGAGGAGAGGAGGTTGCAAGACGTCTGCTAATGAACCCACGGAGTATTCCTTCCCTTCAGAGCTTGGCTGCGGACGGATCCTTGAAAGCTCGAAGAAAAGCCGATGCACTGCTTAGATTACTGAATAGATGCTGCTTTCAATCTCACAATCAGATTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002237 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009608 GO:0009609 GO:0009610 GO:0009617 GO:0009620 GO:0009877 GO:0009987 GO:0010033 GO:0010941 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0033612 GO:0036211 GO:0036377 GO:0042221 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0060548 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

688

Amino Acids

75.54

Weight (kDa)

6.88

Isoelectric Point (pI)

45.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCAfunc	PF19584	43 - 152	7.5e-07	MCAfunc domain
PUB10_N	PF25368	46 - 152	5.8e-15	PUB10-like, N-terminal domain
U-box	PF04564	281 - 352	6.9e-20	U-box domain
zf-Nse	PF11789	284 - 326	7.9e-06	Zinc-finger of the MIZ type in Nse subunit
ARM_PUB	PF25598	413 - 679	2.3e-22	Plant U-box protein ARM repeat region
Arm	PF00514	428 - 465	6.7e-10	Armadillo/beta-catenin-like repeat
ARM_ARMC5	PF24768	428 - 662	9.9e-11	ARMC5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0017717)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g24820
malus_domestica	MD01G1156100.v1.1 MD07G1224800.v1.1
prunus_persica	Prupe.2G253400_v2.0.a1
pyrus_communis	pycom01g17300
rosa_chinensis	RchiOBHm_Chr1g0370691
rosa_laevigata	RLG00000027017
rosa_multiflora	Rmu_sc0004550.1_g000011
rosa_roxburghii	Rroxscaffold_4G00286300
rosa_rugosa	Rorug01G0358500
rosa_samantha	Rh1DG363200
rosa_wichuraiana	Rw1G032400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1927
Acc36I	ACCTGC	1 cut(s)	415
AccB1I	GGYRCC	1 cut(s)	954
AccBSI	CCGCTC	1 cut(s)	789
AclI	AACGTT	1 cut(s)	1056
AclWI	GGATC	8 cut(s)	164, 217, 268, 950, 1149, 1301, 1974, 1987
AcsI	RAATTY	3 cut(s)	743, 851, 925
AcuI	CTGAAG	2 cut(s)	1800, 1943
AcyI	GRCGYC	2 cut(s)	1134, 1924
AfaI	GTAC	1 cut(s)	1127
AfeI	AGCGCT	2 cut(s)	999, 1672
AfiI	CCNNNNNNNGG	3 cut(s)	150, 974, 975
AflII	CTTAAG	2 cut(s)	398, 1837
AgsI	TTSAA	4 cut(s)	1591, 1639, 1987, 2042
AjiI	CACGTC	1 cut(s)	963
Alw21I	GWGCWC	1 cut(s)	299
Alw26I	GTCTC	1 cut(s)	879
AlwI	GGATC	8 cut(s)	164, 217, 268, 950, 1149, 1301, 1974, 1987
AlwNI	CAGNNNCTG	2 cut(s)	985, 1376
Ama87I	CYCGRG	1 cut(s)	790
Aor51HI	AGCGCT	2 cut(s)	999, 1672
AoxI	GGCC	3 cut(s)	127, 202, 586
ApoI	RAATTY	3 cut(s)	743, 851, 925
Asp700I	GAANNNNTTC	1 cut(s)	111
AspLEI	GCGC	2 cut(s)	1000, 1673
AspS9I	GGNCC	2 cut(s)	127, 203
AsuC2I	CCSGG	1 cut(s)	907
AsuHPI	GGTGA	7 cut(s)	262, 364, 415, 431, 1215, 1339, 1678
AsuII	TTCGAA	4 cut(s)	180, 256, 332, 747
AvaI	CYCGRG	1 cut(s)	790
BaeGI	GKGCMC	3 cut(s)	912, 957, 968
BamHI	GGATCC	1 cut(s)	1979
BanI	GGYRCC	1 cut(s)	954
BanII	GRGCYC	2 cut(s)	299, 373
BbsI	GAAGAC	1 cut(s)	1074
Bbv12I	GWGCWC	1 cut(s)	299
BbvCI	CCTCAGC	1 cut(s)	1302
BccI	CCATC	7 cut(s)	480, 508, 731, 1403, 1708, 1780, 1895
BceAI	ACGGC	3 cut(s)	217, 423, 1121
BcgI	CGANNNNNNTGC	2 cut(s)	836, 870
BclI	TGATCA	2 cut(s)	805, 1419
BcnI	CCSGG	1 cut(s)	907
BcoDI	GTCTC	1 cut(s)	879
BfaI	CTAG	2 cut(s)	1211, 1560
BfmI	CTRYAG	2 cut(s)	485, 776
BfoI	RGCGCY	2 cut(s)	1001, 1674
BfrI	CTTAAG	2 cut(s)	398, 1837
BfuAI	ACCTGC	1 cut(s)	415
BlpI	GCTNAGC	1 cut(s)	1761
Bme1390I	CCNGG	1 cut(s)	907
BmeT110I	CYCGRG	1 cut(s)	790
BmgBI	CACGTC	1 cut(s)	963
BmgT120I	GGNCC	2 cut(s)	127, 203
BmiI	GGNNCC	3 cut(s)	956, 1692, 1981
BmrFI	CCNGG	1 cut(s)	907
BmrI	ACTGGG	2 cut(s)	63, 1072
BmsI	GCATC	6 cut(s)	911, 1162, 1609, 1732, 1996, 2021
BmuI	ACTGGG	2 cut(s)	63, 1072
BpiI	GAAGAC	1 cut(s)	1074
BplI	GAGNNNNNCTC	2 cut(s)	230, 262
Bpu10I	CCTNAGC	1 cut(s)	1302
Bpu1102I	GCTNAGC	1 cut(s)	1761
Bpu14I	TTCGAA	4 cut(s)	180, 256, 332, 747
BpuEI	CTTGAG	2 cut(s)	650, 1004
BpuMI	CCSGG	1 cut(s)	907
Bsa29I	ATCGAT	1 cut(s)	1830
BsaAI	YACGTR	1 cut(s)	346
BsaBI	GATNNNNATC	1 cut(s)	1418
BsaHI	GRCGYC	2 cut(s)	1134, 1924
BsaI	GGTCTC	1 cut(s)	879
BsaJI	CCNNGG	6 cut(s)	36, 102, 600, 1137, 1746, 1940
BsaWI	WCCGGW	2 cut(s)	115, 1225
BsaXI	ACNNNNNCTCC	4 cut(s)	1096, 1126, 1899, 1929
Bsc4I	CCNNNNNNNGG	3 cut(s)	150, 974, 975
Bse118I	RCCGGY	2 cut(s)	441, 1274
Bse1I	ACTGG	3 cut(s)	58, 1078, 1222
Bse3DI	GCAATG	1 cut(s)	1331
Bse8I	GATNNNNATC	1 cut(s)	1418
BseCI	ATCGAT	1 cut(s)	1830
BseDI	CCNNGG	6 cut(s)	36, 102, 600, 1137, 1746, 1940
BseGI	GGATG	5 cut(s)	10, 902, 1723, 1744, 1791
BseJI	GATNNNNATC	1 cut(s)	1418
BseLI	CCNNNNNNNGG	3 cut(s)	150, 974, 975
BseMI	GCAATG	1 cut(s)	1331
BseMII	CTCAG	7 cut(s)	312, 380, 566, 581, 1182, 1293, 1775
BseNI	ACTGG	3 cut(s)	58, 1078, 1222
BseRI	GAGGAG	7 cut(s)	12, 155, 1061, 1117, 1688, 1920, 1925
BseSI	GKGCMC	3 cut(s)	912, 957, 968
BseYI	CCCAGC	2 cut(s)	1682, 1885
BsgI	GTGCAG	1 cut(s)	1362
BshFI	GGCC	3 cut(s)	129, 204, 588
BshNI	GGYRCC	1 cut(s)	954
BshVI	ATCGAT	1 cut(s)	1830
BsiHKAI	GWGCWC	1 cut(s)	299
BsiHKCI	CYCGRG	1 cut(s)	790
BsiSI	CCGG	7 cut(s)	116, 130, 442, 906, 1226, 1275, 1604
BslI	CCNNNNNNNGG	3 cut(s)	150, 974, 975
BsmAI	GTCTC	1 cut(s)	879
BsmI	GAATGC	2 cut(s)	1498, 1646
BsnI	GGCC	3 cut(s)	129, 204, 588
Bso31I	GGTCTC	1 cut(s)	879
BsoBI	CYCGRG	1 cut(s)	790
Bsp119I	TTCGAA	4 cut(s)	180, 256, 332, 747
Bsp1286I	GDGCHC	5 cut(s)	299, 373, 912, 957, 968
Bsp1720I	GCTNAGC	1 cut(s)	1761
Bsp19I	CCATGG	1 cut(s)	102
BspANI	GGCC	3 cut(s)	129, 204, 588
BspCNI	CTCAG	7 cut(s)	313, 381, 567, 582, 1183, 1294, 1774
BspDI	ATCGAT	1 cut(s)	1830
BspLI	GGNNCC	3 cut(s)	956, 1692, 1981
BspMAI	CTGCAG	2 cut(s)	489, 780
BspMI	ACCTGC	1 cut(s)	415
BspPI	GGATC	8 cut(s)	164, 217, 268, 950, 1149, 1301, 1974, 1987
BspQI	GCTCTTC	1 cut(s)	492
BspT104I	TTCGAA	4 cut(s)	180, 256, 332, 747
BspT107I	GGYRCC	1 cut(s)	954
BspTI	CTTAAG	2 cut(s)	398, 1837
BspTNI	GGTCTC	1 cut(s)	879
BsrBI	CCGCTC	1 cut(s)	789
BsrDI	GCAATG	1 cut(s)	1331
BsrFI	RCCGGY	2 cut(s)	441, 1274
BsrI	ACTGG	3 cut(s)	58, 1078, 1222
BssAI	RCCGGY	2 cut(s)	441, 1274
BssECI	CCNNGG	6 cut(s)	36, 102, 600, 1137, 1746, 1940
BssNI	GRCGYC	2 cut(s)	1134, 1924
BssT1I	CCWWGG	2 cut(s)	102, 1746
Bst4CI	ACNGT	2 cut(s)	601, 1112
Bst6I	CTCTTC	8 cut(s)	177, 223, 253, 487, 492, 1640, 1785, 1884
BstACI	GRCGYC	2 cut(s)	1134, 1924
BstAFI	CTTAAG	2 cut(s)	398, 1837
BstAPI	GCANNNNNTGC	2 cut(s)	1376, 1757
BstBAI	YACGTR	1 cut(s)	346
BstBI	TTCGAA	4 cut(s)	180, 256, 332, 747
BstDEI	CTNAG	9 cut(s)	321, 389, 575, 590, 1191, 1256, 1302, 1761, 2015
BstDSI	CCRYGG	5 cut(s)	36, 102, 600, 1137, 1940
BstF5I	GGATG	5 cut(s)	10, 902, 1723, 1744, 1791
BstH2I	RGCGCY	2 cut(s)	1001, 1674
BstHHI	GCGC	2 cut(s)	1000, 1673
BstMAI	GTCTC	1 cut(s)	879
BstSCI	CCNGG	1 cut(s)	905
BstSFI	CTRYAG	2 cut(s)	485, 776
BstSLI	GKGCMC	3 cut(s)	912, 957, 968
BstV2I	GAAGAC	1 cut(s)	1074
BstX2I	RGATCY	1 cut(s)	1979
BstYI	RGATCY	1 cut(s)	1979
Bsu15I	ATCGAT	1 cut(s)	1830
BsuRI	GGCC	3 cut(s)	129, 204, 588
BsuTUI	ATCGAT	1 cut(s)	1830
BtgI	CCRYGG	5 cut(s)	36, 102, 600, 1137, 1940
BtrI	CACGTC	1 cut(s)	963
BtsCI	GGATG	5 cut(s)	10, 902, 1723, 1744, 1791
BtsI	GCAGTG	4 cut(s)	785, 1379, 1758, 2009
BtsIMutI	CAGTG	5 cut(s)	386, 785, 1379, 1758, 2009
BveI	ACCTGC	1 cut(s)	415
CaiI	CAGNNNCTG	2 cut(s)	985, 1376
CfoI	GCGC	2 cut(s)	1000, 1673
Cfr10I	RCCGGY	2 cut(s)	441, 1274
Cfr13I	GGNCC	2 cut(s)	127, 203
ClaI	ATCGAT	1 cut(s)	1830
CseI	GACGC	1 cut(s)	1142
Csp6I	GTAC	1 cut(s)	1126
CspCI	CAANNNNNGTGG	2 cut(s)	375, 410
CviAII	CATG	7 cut(s)	103, 145, 848, 1279, 1346, 1423, 1522
CviQI	GTAC	1 cut(s)	1126
DdeI	CTNAG	9 cut(s)	321, 389, 575, 590, 1191, 1256, 1302, 1761, 2015
Eam1104I	CTCTTC	8 cut(s)	177, 223, 253, 487, 492, 1640, 1785, 1884
EarI	CTCTTC	8 cut(s)	177, 223, 253, 487, 492, 1640, 1785, 1884
Ecl136II	GAGCTC	1 cut(s)	297
Eco130I	CCWWGG	2 cut(s)	102, 1746
Eco24I	GRGCYC	2 cut(s)	299, 373
Eco31I	GGTCTC	1 cut(s)	879
Eco32I	GATATC	1 cut(s)	1231
Eco47III	AGCGCT	2 cut(s)	999, 1672
Eco53kI	GAGCTC	1 cut(s)	297
Eco57I	CTGAAG	2 cut(s)	1800, 1943
Eco88I	CYCGRG	1 cut(s)	790
EcoICRI	GAGCTC	1 cut(s)	297
EcoRI	GAATTC	1 cut(s)	743
EcoRV	GATATC	1 cut(s)	1231
EcoT14I	CCWWGG	2 cut(s)	102, 1746
EcoT38I	GRGCYC	2 cut(s)	299, 373
ErhI	CCWWGG	2 cut(s)	102, 1746
FaeI	CATG	7 cut(s)	106, 148, 851, 1282, 1349, 1426, 1525
FatI	CATG	7 cut(s)	102, 144, 847, 1278, 1345, 1422, 1521
FauI	CCCGC	3 cut(s)	213, 968, 1300
FauNDI	CATATG	1 cut(s)	83
FbaI	TGATCA	2 cut(s)	805, 1419
FokI	GGATG	5 cut(s)	17, 889, 1730, 1751, 1798
FriOI	GRGCYC	2 cut(s)	299, 373
FspBI	CTAG	2 cut(s)	1211, 1560
GlaI	GCGC	2 cut(s)	999, 1672
GsaI	CCCAGC	2 cut(s)	1686, 1889
HaeII	RGCGCY	2 cut(s)	1001, 1674
HaeIII	GGCC	3 cut(s)	129, 204, 588
HapII	CCGG	7 cut(s)	116, 130, 442, 906, 1226, 1275, 1604
HgaI	GACGC	1 cut(s)	1142
HhaI	GCGC	2 cut(s)	1000, 1673
Hin1I	GRCGYC	2 cut(s)	1134, 1924
Hin1II	CATG	7 cut(s)	106, 148, 851, 1282, 1349, 1426, 1525
Hin6I	GCGC	2 cut(s)	998, 1671
HinP1I	GCGC	2 cut(s)	998, 1671
HindIII	AAGCTT	2 cut(s)	110, 626
HpaII	CCGG	7 cut(s)	116, 130, 442, 906, 1226, 1275, 1604
HphI	GGTGA	7 cut(s)	262, 364, 415, 431, 1215, 1339, 1678
Hpy166II	GTNNAC	2 cut(s)	79, 1453
Hpy188I	TCNGA	7 cut(s)	273, 576, 1093, 1419, 1780, 1962, 2055
Hpy188III	TCNNGA	2 cut(s)	374, 1021
Hpy8I	GTNNAC	2 cut(s)	79, 1453
HpyAV	CCTTC	7 cut(s)	349, 1284, 1589, 1775, 1855, 1962, 1967
HpyCH4III	ACNGT	2 cut(s)	601, 1112
HpyCH4IV	ACGT	5 cut(s)	345, 962, 1056, 1455, 1924
HpyF3I	CTNAG	9 cut(s)	321, 389, 575, 590, 1191, 1256, 1302, 1761, 2015
HpySE526I	ACGT	5 cut(s)	345, 962, 1056, 1455, 1924
Hsp92I	GRCGYC	2 cut(s)	1134, 1924
Hsp92II	CATG	7 cut(s)	106, 148, 851, 1282, 1349, 1426, 1525
HspAI	GCGC	2 cut(s)	998, 1671
Ksp22I	TGATCA	2 cut(s)	805, 1419
LguI	GCTCTTC	1 cut(s)	492
LmnI	GCTCC	8 cut(s)	104, 168, 238, 294, 460, 1104, 1310, 1433
LweI	GCATC	6 cut(s)	911, 1162, 1609, 1732, 1996, 2021
MaeI	CTAG	2 cut(s)	1211, 1560
MaeII	ACGT	5 cut(s)	345, 962, 1056, 1455, 1924
MaeIII	GTNAC	5 cut(s)	63, 403, 1327, 1372, 1540
MbiI	CCGCTC	1 cut(s)	789
MfeI	CAATTG	1 cut(s)	1437
MflI	RGATCY	1 cut(s)	1979
MhlI	GDGCHC	5 cut(s)	299, 373, 912, 957, 968
MluCI	AATT	9 cut(s)	359, 743, 851, 925, 946, 1121, 1181, 1437, 1506
MlyI	GAGTC	2 cut(s)	270, 1033
MroXI	GAANNNNTTC	1 cut(s)	111
MseI	TTAA	3 cut(s)	399, 468, 1838
MslI	CAYNNNNRTG	1 cut(s)	915
MspA1I	CMGCKG	2 cut(s)	660, 1190
MspCI	CTTAAG	2 cut(s)	398, 1837
MspI	CCGG	7 cut(s)	116, 130, 442, 906, 1226, 1275, 1604
MspR9I	CCNGG	1 cut(s)	907
MunI	CAATTG	1 cut(s)	1437
Mva1269I	GAATGC	2 cut(s)	1498, 1646
NciI	CCSGG	1 cut(s)	907
NcoI	CCATGG	1 cut(s)	102
NdeI	CATATG	1 cut(s)	83
NlaIII	CATG	7 cut(s)	106, 148, 851, 1282, 1349, 1426, 1525
NlaIV	GGNNCC	3 cut(s)	956, 1692, 1981
NmuCI	GTSAC	2 cut(s)	403, 1327
NspV	TTCGAA	4 cut(s)	180, 256, 332, 747
PciSI	GCTCTTC	1 cut(s)	492
PcsI	WCGNNNNNNNCGW	1 cut(s)	1134
PctI	GAATGC	2 cut(s)	1498, 1646
PdmI	GAANNNNTTC	1 cut(s)	111
PflFI	GACNNNGTC	1 cut(s)	55
PleI	GAGTC	2 cut(s)	269, 1032
PpsI	GAGTC	2 cut(s)	269, 1032
Ppu21I	YACGTR	1 cut(s)	346
Psp124BI	GAGCTC	1 cut(s)	299
Psp1406I	AACGTT	1 cut(s)	1056
PspFI	CCCAGC	2 cut(s)	1682, 1885
PspN4I	GGNNCC	3 cut(s)	956, 1692, 1981
PspPI	GGNCC	2 cut(s)	127, 203
PsrI	GAACNNNNNNTAC	3 cut(s)	32, 1341, 1373
PstI	CTGCAG	2 cut(s)	489, 780
PstNI	CAGNNNCTG	2 cut(s)	985, 1376
PsuI	RGATCY	1 cut(s)	1979
PsyI	GACNNNGTC	1 cut(s)	55
PvuII	CAGCTG	1 cut(s)	1190
RsaI	GTAC	1 cut(s)	1127
RsaNI	GTAC	1 cut(s)	1126
RseI	CAYNNNNRTG	1 cut(s)	915
SacI	GAGCTC	1 cut(s)	299
SapI	GCTCTTC	1 cut(s)	492
SaqAI	TTAA	3 cut(s)	399, 468, 1838
Sau96I	GGNCC	2 cut(s)	127, 203
SchI	GAGTC	2 cut(s)	270, 1033
ScrFI	CCNGG	1 cut(s)	907
SduI	GDGCHC	5 cut(s)	299, 373, 912, 957, 968
SfaNI	GCATC	6 cut(s)	911, 1162, 1609, 1732, 1996, 2021
SfcI	CTRYAG	2 cut(s)	485, 776
SfuI	TTCGAA	4 cut(s)	180, 256, 332, 747
SmiMI	CAYNNNNRTG	1 cut(s)	915
SmlI	CTYRAG	4 cut(s)	398, 629, 1019, 1837
SmoI	CTYRAG	4 cut(s)	398, 629, 1019, 1837
Sse9I	AATT	9 cut(s)	359, 743, 851, 925, 946, 1121, 1181, 1437, 1506
SspMI	CTAG	2 cut(s)	1211, 1560
SstI	GAGCTC	1 cut(s)	299
StyD4I	CCNGG	1 cut(s)	905
StyI	CCWWGG	2 cut(s)	102, 1746
TaaI	ACNGT	2 cut(s)	601, 1112
TaiI	ACGT	5 cut(s)	348, 965, 1059, 1458, 1927
TaqII	GACCGA	1 cut(s)	936
TasI	AATT	9 cut(s)	359, 743, 851, 925, 946, 1121, 1181, 1437, 1506
TauI	GCSGC	2 cut(s)	1247, 1430
Tru1I	TTAA	3 cut(s)	399, 468, 1838
Tru9I	TTAA	3 cut(s)	399, 468, 1838
TscAI	CASTG	5 cut(s)	393, 785, 1386, 1758, 2016
TseFI	GTSAC	2 cut(s)	403, 1327
Tsp45I	GTSAC	2 cut(s)	403, 1327
TspDTI	ATGAA	8 cut(s)	535, 756, 785, 804, 858, 864, 1362, 1949
TspGWI	ACGGA	5 cut(s)	251, 1308, 1751, 1957, 1992
TspRI	CASTG	5 cut(s)	393, 785, 1386, 1758, 2016
Tth111I	GACNNNGTC	1 cut(s)	55
Vha464I	CTTAAG	2 cut(s)	398, 1837
XapI	RAATTY	3 cut(s)	743, 851, 925
XmnI	GAANNNNTTC	1 cut(s)	111
XspI	CTAG	2 cut(s)	1211, 1560
ZraI	GACGTC	1 cut(s)	1925

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.