Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008710

Physical Location & Seq

Genomic Coordinates Forward (+)

27000183 .. 27003189

Gene Structure 3007 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1166300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGATTCGGCAACCCAACAAAGAGAGAAGCGTCAGAGAGTGGATGCTGAAGCTACTGATATCATGGATTACGATGTTTCAGATGATGGGATTGAAGGTTCTTCTTCTCCCAAAAAGCTGAAAGAGAATGGACATTCTTCCCTTCCTACTCCATCTCAGCAGTCAATTACTGAGGGCACCAGCTCTAGTGTGATTATGGTGCCTGATGTCGACAGGGATTTGCTTCCTTGTCTTATTAAGATATATGATTGTCCGGAGTCTGACTTAAAGCTAAATGAGGTTTTTGAGTTTGTTGGAGTCTTCACTTTTGATTCAGAATTTAAAGAGGACAAGGATGTATCAGATGATTTCACAAATGGTTTTAGCGAAGATGTGTTGGTTCACTTGCCCCCTAATAAGGTACCACGTCTCCACTGTTTTATTCACAGGAAACTTGCAGTTCACGACTTCCTTCCTTGTTCCCCTACAATCGAGCCAAAACCCAACGTGGTGAAAGAGATCAGGGAGTCTCTCTTGAGGCACCTTACAGCTGTTCTTGGTAATGATGGCATAGCTGCTAATTTCATGCTGTTGCATCTCTTGTCCACGGTGCATTCTAGAGTTGAAACTGTCGCTGTGGGGAAGCTTTCACTAAATCTTAATTGTTTAAGCAAAGAAAGTGCACCCGTGTTTGGTACTCGGATTGGCCGTGCTCTCAAGAATATTTTACCTTTCACACAATGTATATCCCTAACAGTGGATTATCTCAACACTGCTTCTCTTGCGCCAAGGAAGGATTATGAGACAAGCAGGCTGATAACTGGAGCTCTGCAGCTGGCTGAGGGTTCACACATGATATTTGATGAGACCCGGTTGGAAGCTGGCATTCTTAACTCTGTTGGGGTTGAAAATGCAAGATTGCTTAAAACTTTAATAGAGTTGCAAAAGGTGGAATATGATTTCAAGTTCTATAAACTGGACATGCCAGCAGACATTCAAATGGTTGTTCTTTCAGAAGGCAAATCAAACATTTTGCCAGCGGATGTGGTGTTACCTTTCCACCCTTCTTCAGTGGCTTCTGTTGAAGTTACCGCAGAAGCACTAGAGGTTTGGAGATGGTACTTGGCTACTCTTAGATCATTGCCACATTCTATTGAGTCAGAAATACAGAAGGTGATAGAAAATGACTTGGTTGCGGCAAGGCAAGAAGATAGGACACTGGGAACCCAAGATTTCAGCAGATTGCTGACAATGGGTCGATTGATGTCTATGAGTTTCGGTGAAACCTCCCTATCATTAGAACACTGGCAGATGGTCAAGGAACTCGAGAGGTTAAGAAGAGAGAGACTCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0000819 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016043 GO:0022402 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM_bind

Protein Analysis

445

Amino Acids

49.86

Weight (kDa)

5.24

Isoelectric Point (pI)

47.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_bind	PF09739	3 - 443	1.8e-150	Mini-chromosome maintenance replisome factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40550
fragaria_vesca	FvH4_7g25850 FvH4_7g25850 FvH4_7g25850 FvH4_7g25850 FvH4_7g25850
malus_domestica	MD01G1166300.v1.1 MD01G1183300.v1.1 MD07G1233000.v1.1
prunus_persica	Prupe.2G263500_v2.0.a1 Prupe.2G263500_v2.0.a1
pyrus_communis	pycom01g17990 pycom07g21080
rosa_chinensis	RchiOBHm_Chr1g0372121
rosa_laevigata	RLG00000026921
rosa_multiflora	Rmu_sc0001755.1_g000024
rosa_roxburghii	Rroxscaffold_4G00285230
rosa_rugosa	Rorug01G0369900
rosa_samantha	Rh1AG378300 Rh1BG341600 Rh1CG355000 Rh1DG372700
rosa_wichuraiana	Rw1G033280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	400
AccB1I	GGYRCC	4 cut(s)	176, 199, 400, 519
AccI	GTMKAC	1 cut(s)	210
AccIII	TCCGGA	1 cut(s)	253
AciI	CCGC	3 cut(s)	1019, 1071, 1175
AcoI	YGGCCR	1 cut(s)	685
AcsI	RAATTY	1 cut(s)	317
AcuI	CTGAAG	2 cut(s)	69, 1032
AfaI	GTAC	3 cut(s)	402, 676, 1100
AfiI	CCNNNNNNNGG	3 cut(s)	397, 671, 736
AgsI	TTSAA	6 cut(s)	95, 605, 887, 943, 977, 1064
AhdI	GACNNNNNGTC	1 cut(s)	1233
AjiI	CACGTC	1 cut(s)	407
AloI	GAACNNNNNNTCC	2 cut(s)	82, 114
Alw21I	GWGCWC	3 cut(s)	664, 694, 808
Alw26I	GTCTC	5 cut(s)	413, 513, 776, 839, 1318
Alw44I	GTGCAC	1 cut(s)	660
Ama87I	CYCGRG	1 cut(s)	1304
Aor13HI	TCCGGA	1 cut(s)	253
AoxI	GGCC	1 cut(s)	685
ApaLI	GTGCAC	1 cut(s)	660
ApeKI	GCWGC	2 cut(s)	554, 811
ApoI	RAATTY	1 cut(s)	317
Asp718I	GGTACC	1 cut(s)	400
AspLEI	GCGC	1 cut(s)	766
AsuC2I	CCSGG	1 cut(s)	850
AsuHPI	GGTGA	3 cut(s)	502, 1165, 1271
AvaI	CYCGRG	1 cut(s)	1304
BaeGI	GKGCMC	2 cut(s)	179, 664
BanI	GGYRCC	4 cut(s)	176, 199, 400, 519
BanII	GRGCYC	1 cut(s)	808
BbsI	GAAGAC	1 cut(s)	292
Bbv12I	GWGCWC	3 cut(s)	664, 694, 808
BbvCI	CCTCAGC	1 cut(s)	819
BbvI	GCAGC	2 cut(s)	541, 823
BccI	CCATC	5 cut(s)	80, 160, 539, 1089, 1285
BceAI	ACGGC	1 cut(s)	672
BcnI	CCSGG	1 cut(s)	850
BcoDI	GTCTC	5 cut(s)	413, 513, 776, 839, 1318
BfaI	CTAG	3 cut(s)	186, 597, 1082
BfmI	CTRYAG	1 cut(s)	809
BisI	GCNGC	3 cut(s)	555, 812, 1176
BlsI	GCNGC	3 cut(s)	556, 813, 1177
Bme1390I	CCNGG	1 cut(s)	850
BmeRI	GACNNNNNGTC	1 cut(s)	1233
BmeT110I	CYCGRG	1 cut(s)	1304
BmgBI	CACGTC	1 cut(s)	407
BmiI	GGNNCC	5 cut(s)	178, 201, 402, 521, 1204
BmrFI	CCNGG	1 cut(s)	850
BmrI	ACTGGG	1 cut(s)	1208
BmsI	GCATC	2 cut(s)	34, 583
BmuI	ACTGGG	1 cut(s)	1208
BpiI	GAAGAC	1 cut(s)	292
BplI	GAGNNNNNCTC	1 cut(s)	1311
BpmI	CTGGAG	1 cut(s)	822
Bpu10I	CCTNAGC	1 cut(s)	819
BpuEI	CTTGAG	3 cut(s)	535, 680, 1313
BpuMI	CCSGG	1 cut(s)	850
BsaI	GGTCTC	1 cut(s)	839
BsaJI	CCNNGG	2 cut(s)	585, 767
BsaWI	WCCGGW	1 cut(s)	253
BsaXI	ACNNNNNCTCC	2 cut(s)	393, 423
Bsc4I	CCNNNNNNNGG	3 cut(s)	397, 671, 736
Bse1I	ACTGG	4 cut(s)	805, 960, 1203, 1289
Bse3DI	GCAATG	1 cut(s)	1118
BseAI	TCCGGA	1 cut(s)	253
BseDI	CCNNGG	2 cut(s)	585, 767
BseGI	GGATG	3 cut(s)	49, 340, 1027
BseLI	CCNNNNNNNGG	3 cut(s)	397, 671, 736
BseMI	GCAATG	1 cut(s)	1118
BseMII	CTCAG	3 cut(s)	162, 170, 810
BseNI	ACTGG	4 cut(s)	805, 960, 1203, 1289
BseSI	GKGCMC	2 cut(s)	179, 664
BseXI	GCAGC	2 cut(s)	541, 823
BshFI	GGCC	1 cut(s)	687
BshNI	GGYRCC	4 cut(s)	176, 199, 400, 519
BsiHKAI	GWGCWC	3 cut(s)	664, 694, 808
BsiHKCI	CYCGRG	1 cut(s)	1304
BsiSI	CCGG	2 cut(s)	254, 850
BslI	CCNNNNNNNGG	3 cut(s)	397, 671, 736
BsmAI	GTCTC	5 cut(s)	413, 513, 776, 839, 1318
BsmBI	CGTCTC	1 cut(s)	413
BsmI	GAATGC	2 cut(s)	592, 864
BsnI	GGCC	1 cut(s)	687
Bso31I	GGTCTC	1 cut(s)	839
BsoBI	CYCGRG	1 cut(s)	1304
Bsp1286I	GDGCHC	4 cut(s)	179, 664, 694, 808
Bsp13I	TCCGGA	1 cut(s)	253
Bsp143I	GATC	2 cut(s)	498, 1115
BspACI	CCGC	3 cut(s)	1019, 1071, 1175
BspANI	GGCC	1 cut(s)	687
BspCNI	CTCAG	3 cut(s)	163, 169, 811
BspEI	TCCGGA	1 cut(s)	253
BspLI	GGNNCC	5 cut(s)	178, 201, 402, 521, 1204
BspMAI	CTGCAG	1 cut(s)	813
BspT107I	GGYRCC	4 cut(s)	176, 199, 400, 519
BspTNI	GGTCTC	1 cut(s)	839
BsrDI	GCAATG	1 cut(s)	1118
BsrI	ACTGG	4 cut(s)	805, 960, 1203, 1289
BssECI	CCNNGG	2 cut(s)	585, 767
BssMI	GATC	2 cut(s)	498, 1115
BssT1I	CCWWGG	1 cut(s)	767
Bst4CI	ACNGT	4 cut(s)	416, 589, 610, 736
Bst6I	CTCTTC	1 cut(s)	1312
BstC8I	GCNNGC	5 cut(s)	791, 816, 862, 966, 1017
BstDEI	CTNAG	4 cut(s)	156, 171, 819, 1112
BstDSI	CCRYGG	1 cut(s)	585
BstF5I	GGATG	3 cut(s)	49, 340, 1027
BstHHI	GCGC	1 cut(s)	766
BstKTI	GATC	2 cut(s)	501, 1118
BstMAI	GTCTC	5 cut(s)	413, 513, 776, 839, 1318
BstMBI	GATC	2 cut(s)	498, 1115
BstMWI	GCNNNNNNNGC	1 cut(s)	761
BstNSI	RCATGY	1 cut(s)	964
BstSCI	CCNGG	1 cut(s)	848
BstSFI	CTRYAG	1 cut(s)	809
BstSLI	GKGCMC	2 cut(s)	179, 664
BstV1I	GCAGC	2 cut(s)	541, 823
BstV2I	GAAGAC	1 cut(s)	292
BsuRI	GGCC	1 cut(s)	687
BtgI	CCRYGG	1 cut(s)	585
BtrI	CACGTC	1 cut(s)	407
BtsCI	GGATG	3 cut(s)	49, 340, 1027
BtsI	GCAGTG	1 cut(s)	750
BtsIMutI	CAGTG	6 cut(s)	412, 741, 750, 1056, 1196, 1282
Cac8I	GCNNGC	5 cut(s)	791, 816, 862, 966, 1017
CfoI	GCGC	1 cut(s)	766
CseI	GACGC	1 cut(s)	20
Csp6I	GTAC	3 cut(s)	401, 675, 1099
CviAII	CATG	4 cut(s)	64, 565, 832, 961
CviQI	GTAC	3 cut(s)	401, 675, 1099
DdeI	CTNAG	4 cut(s)	156, 171, 819, 1112
DpnI	GATC	2 cut(s)	500, 1117
DpnII	GATC	2 cut(s)	498, 1115
DraI	TTTAAA	1 cut(s)	322
DriI	GACNNNNNGTC	1 cut(s)	1233
EaeI	YGGCCR	1 cut(s)	685
Eam1104I	CTCTTC	1 cut(s)	1312
Eam1105I	GACNNNNNGTC	1 cut(s)	1233
EarI	CTCTTC	1 cut(s)	1312
Ecl136II	GAGCTC	1 cut(s)	806
Eco130I	CCWWGG	1 cut(s)	767
Eco24I	GRGCYC	1 cut(s)	808
Eco31I	GGTCTC	1 cut(s)	839
Eco32I	GATATC	1 cut(s)	61
Eco53kI	GAGCTC	1 cut(s)	806
Eco57I	CTGAAG	2 cut(s)	69, 1032
Eco88I	CYCGRG	1 cut(s)	1304
EcoICRI	GAGCTC	1 cut(s)	806
EcoRV	GATATC	1 cut(s)	61
EcoT14I	CCWWGG	1 cut(s)	767
EcoT38I	GRGCYC	1 cut(s)	808
ErhI	CCWWGG	1 cut(s)	767
Esp3I	CGTCTC	1 cut(s)	413
FaeI	CATG	4 cut(s)	67, 568, 835, 964
FatI	CATG	4 cut(s)	63, 564, 831, 960
FblI	GTMKAC	1 cut(s)	210
Fnu4HI	GCNGC	3 cut(s)	555, 812, 1176
FokI	GGATG	3 cut(s)	56, 347, 1034
FriOI	GRGCYC	1 cut(s)	808
Fsp4HI	GCNGC	3 cut(s)	555, 812, 1176
FspBI	CTAG	3 cut(s)	186, 597, 1082
GlaI	GCGC	1 cut(s)	765
GluI	GCNGC	3 cut(s)	555, 812, 1176
GsuI	CTGGAG	1 cut(s)	822
HaeIII	GGCC	1 cut(s)	687
HapII	CCGG	2 cut(s)	254, 850
HgaI	GACGC	1 cut(s)	20
HhaI	GCGC	1 cut(s)	766
Hin1II	CATG	4 cut(s)	67, 568, 835, 964
Hin6I	GCGC	1 cut(s)	764
HinP1I	GCGC	1 cut(s)	764
HincII	GTYRAC	1 cut(s)	211
HindII	GTYRAC	1 cut(s)	211
HindIII	AAGCTT	1 cut(s)	623
HinfI	GANTC	7 cut(s)	5, 257, 297, 311, 506, 1136, 1326
HpaII	CCGG	2 cut(s)	254, 850
HphI	GGTGA	3 cut(s)	502, 1165, 1271
Hpy166II	GTNNAC	6 cut(s)	211, 382, 442, 585, 662, 827
Hpy188I	TCNGA	8 cut(s)	36, 82, 262, 316, 343, 681, 994, 1141
Hpy188III	TCNNGA	6 cut(s)	254, 443, 514, 597, 697, 1306
Hpy8I	GTNNAC	6 cut(s)	211, 382, 442, 585, 662, 827
HpyAV	CCTTC	7 cut(s)	89, 152, 461, 766, 989, 1053, 1144
HpyCH4III	ACNGT	4 cut(s)	416, 589, 610, 736
HpyCH4IV	ACGT	2 cut(s)	406, 486
HpyCH4V	TGCA	7 cut(s)	437, 574, 592, 662, 811, 893, 922
HpyF10VI	GCNNNNNNNGC	1 cut(s)	761
HpyF3I	CTNAG	4 cut(s)	156, 171, 819, 1112
HpySE526I	ACGT	2 cut(s)	406, 486
Hsp92II	CATG	4 cut(s)	67, 568, 835, 964
HspAI	GCGC	1 cut(s)	764
Kpn2I	TCCGGA	1 cut(s)	253
KpnI	GGTACC	1 cut(s)	404
Kzo9I	GATC	2 cut(s)	498, 1115
LmnI	GCTCC	1 cut(s)	803
Lsp1109I	GCAGC	2 cut(s)	541, 823
LweI	GCATC	2 cut(s)	34, 583
MaeI	CTAG	3 cut(s)	186, 597, 1082
MaeII	ACGT	2 cut(s)	406, 486
MaeIII	GTNAC	2 cut(s)	1029, 1066
MalI	GATC	2 cut(s)	500, 1117
MboI	GATC	2 cut(s)	498, 1115
MboII	GAAGA	8 cut(s)	93, 96, 129, 292, 380, 1038, 1199, 1329
MhlI	GDGCHC	4 cut(s)	179, 664, 694, 808
MluCI	AATT	4 cut(s)	165, 317, 559, 640
MlyI	GAGTC	5 cut(s)	266, 306, 515, 1145, 1320
MmeI	TCCRAC	2 cut(s)	274, 834
MnlI	CCTC	8 cut(s)	166, 271, 319, 510, 814, 1078, 1276, 1302
MroI	TCCGGA	1 cut(s)	253
MseI	TTAA	9 cut(s)	237, 266, 321, 639, 647, 870, 903, 911, 1313
MslI	CAYNNNNRTG	1 cut(s)	977
MspA1I	CMGCKG	3 cut(s)	530, 814, 1019
MspI	CCGG	2 cut(s)	254, 850
MspR9I	CCNGG	1 cut(s)	850
Mva1269I	GAATGC	2 cut(s)	592, 864
MwoI	GCNNNNNNNGC	1 cut(s)	761
NciI	CCSGG	1 cut(s)	850
NdeII	GATC	2 cut(s)	498, 1115
NlaIII	CATG	4 cut(s)	67, 568, 835, 964
NlaIV	GGNNCC	5 cut(s)	178, 201, 402, 521, 1204
NspI	RCATGY	1 cut(s)	964
PaeR7I	CTCGAG	1 cut(s)	1304
PcsI	WCGNNNNNNNCGW	1 cut(s)	685
PctI	GAATGC	2 cut(s)	592, 864
PfeI	GAWTC	2 cut(s)	5, 311
PkrI	GCNGC	3 cut(s)	556, 813, 1177
PleI	GAGTC	5 cut(s)	265, 305, 514, 1144, 1320
PpsI	GAGTC	5 cut(s)	265, 305, 514, 1144, 1320
Psp124BI	GAGCTC	1 cut(s)	808
PspN4I	GGNNCC	5 cut(s)	178, 201, 402, 521, 1204
PstI	CTGCAG	1 cut(s)	813
PvuII	CAGCTG	2 cut(s)	530, 814
RsaI	GTAC	3 cut(s)	402, 676, 1100
RsaNI	GTAC	3 cut(s)	401, 675, 1099
RseI	CAYNNNNRTG	1 cut(s)	977
SacI	GAGCTC	1 cut(s)	808
SalI	GTCGAC	1 cut(s)	209
SaqAI	TTAA	9 cut(s)	237, 266, 321, 639, 647, 870, 903, 911, 1313
SatI	GCNGC	3 cut(s)	555, 812, 1176
Sau3AI	GATC	2 cut(s)	498, 1115
SchI	GAGTC	5 cut(s)	266, 306, 515, 1145, 1320
ScrFI	CCNGG	1 cut(s)	850
SduI	GDGCHC	4 cut(s)	179, 664, 694, 808
SfaNI	GCATC	2 cut(s)	34, 583
SfcI	CTRYAG	1 cut(s)	809
Sfr274I	CTCGAG	1 cut(s)	1304
SlaI	CTCGAG	1 cut(s)	1304
SmiMI	CAYNNNNRTG	1 cut(s)	977
SmlI	CTYRAG	4 cut(s)	514, 695, 1304, 1328
SmoI	CTYRAG	4 cut(s)	514, 695, 1304, 1328
Sse9I	AATT	4 cut(s)	165, 317, 559, 640
SsiI	CCGC	3 cut(s)	1019, 1071, 1175
SspI	AATATT	1 cut(s)	703
SspMI	CTAG	3 cut(s)	186, 597, 1082
SstI	GAGCTC	1 cut(s)	808
StyD4I	CCNGG	1 cut(s)	848
StyI	CCWWGG	1 cut(s)	767
TaaI	ACNGT	4 cut(s)	416, 589, 610, 736
TaiI	ACGT	2 cut(s)	409, 489
TaqI	TCGA	4 cut(s)	210, 471, 1237, 1305
TasI	AATT	4 cut(s)	165, 317, 559, 640
TauI	GCSGC	1 cut(s)	1178
TfiI	GAWTC	2 cut(s)	5, 311
Tru1I	TTAA	9 cut(s)	237, 266, 321, 639, 647, 870, 903, 911, 1313
Tru9I	TTAA	9 cut(s)	237, 266, 321, 639, 647, 870, 903, 911, 1313
TscAI	CASTG	6 cut(s)	419, 741, 757, 1056, 1203, 1289
TseI	GCWGC	2 cut(s)	554, 811
TspDTI	ATGAA	1 cut(s)	553
TspRI	CASTG	6 cut(s)	419, 741, 757, 1056, 1203, 1289
VneI	GTGCAC	1 cut(s)	660
XapI	RAATTY	1 cut(s)	317
XbaI	TCTAGA	1 cut(s)	596
XceI	RCATGY	1 cut(s)	964
XhoI	CTCGAG	1 cut(s)	1304
XmiI	GTMKAC	1 cut(s)	210
XspI	CTAG	3 cut(s)	186, 597, 1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.