MD01G1170700.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008881
Physical Location & Seq
Reverse (-)
27382037 .. 27382372
336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1170700.v1.1.491

Sequence Viewer

Length: 336 bp
ATGAAGCTTCAGGTCTCCATTTGCACGCTTATCTTCCTTTTCCTTATTCCGCCTTTCCCTTCCTCAGTTTTTTCTGACCTGAACTCGGATAGACAAGCCCTCCTCAACTTTGCTGCCACCGTTGTCCACATCCGAAAACTCAACTGGAATGCTTCCACGCCAGTTTGCATGTCTTGGGTTGGAATCACTTGCAATCTAAACAAAACGAGTGACTGCCATCCATCTTCCGGGGATTGGACTTTTCGGTTCCATCCCTTCCAACAGTATAGGGAAGCTTCATACTCTTCAAGTCCTCAGCCTCCGCTCCAACTTCCTCTATGGAAGTCTGCCTTCTGA

Protein Analysis

112

Amino Acids

12.62

Weight (kDa)

8.84

Isoelectric Point (pI)

60.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 65 2.4e-09 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 304
AciI CCGC 2 cut(s) 50, 302
AfiI CCNNNNNNNGG 3 cut(s) 85, 227, 234
AgsI TTSAA 1 cut(s) 288
AluBI AGCT 2 cut(s) 7, 275
AluI AGCT 2 cut(s) 7, 275
Alw26I GTCTC 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 100
BccI CCATC 3 cut(s) 225, 229, 258
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 19
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 229
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 229
Bpu10I CCTNAGC 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 229
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 227, 234
Bse1I ACTGG 2 cut(s) 149, 161
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 3 cut(s) 129, 217, 250
BseLI CCNNNNNNNGG 3 cut(s) 85, 227, 234
BseMII CTCAG 2 cut(s) 78, 308
BseNI ACTGG 2 cut(s) 149, 161
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 3 cut(s) 85, 227, 234
BsmAI GTCTC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 154
Bso31I GGTCTC 1 cut(s) 19
BspACI CCGC 2 cut(s) 50, 302
BspCNI CTCAG 2 cut(s) 77, 307
BspLI GGNNCC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 19
BsrBI CCGCTC 1 cut(s) 304
BsrI ACTGG 2 cut(s) 149, 161
BssECI CCNNGG 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 121, 264
Bst6I CTCTTC 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 64, 294
BstF5I GGATG 3 cut(s) 129, 217, 250
BstMAI GTCTC 1 cut(s) 19
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 100
BtsCI GGATG 3 cut(s) 129, 217, 250
Cac8I GCNNGC 1 cut(s) 26
CviAII CATG 1 cut(s) 169
CviJI RGCY 4 cut(s) 7, 98, 275, 298
CviKI_1 RGCY 4 cut(s) 7, 98, 275, 298
DdeI CTNAG 2 cut(s) 64, 294
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
EciI GGCGGA 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 19
FaeI CATG 1 cut(s) 172
FaiI YATR 4 cut(s) 170, 267, 280, 319
FalI AAGNNNNNCTT 1 cut(s) 314
FatI CATG 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 3 cut(s) 116, 204, 237
Fsp4HI GCNGC 1 cut(s) 114
GluI GCNGC 1 cut(s) 114
HapII CCGG 1 cut(s) 228
Hin1II CATG 1 cut(s) 172
HindIII AAGCTT 2 cut(s) 5, 273
HinfI GANTC 1 cut(s) 183
HpaII CCGG 1 cut(s) 228
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 4 cut(s) 76, 88, 134, 335
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 2 cut(s) 69, 265
HpyCH4III ACNGT 2 cut(s) 121, 264
HpyCH4V TGCA 3 cut(s) 24, 168, 192
HpyF3I CTNAG 2 cut(s) 64, 294
Hsp92II CATG 1 cut(s) 172
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 4 cut(s) 92, 130, 174, 241
Lsp1109I GCAGC 1 cut(s) 100
MaeIII GTNAC 1 cut(s) 209
MbiI CCGCTC 1 cut(s) 304
MboII GAAGA 3 cut(s) 25, 216, 276
MmeI TCCRAC 3 cut(s) 160, 283, 331
MnlI CCTC 6 cut(s) 73, 110, 113, 303, 309, 324
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 154
NciI CCSGG 1 cut(s) 229
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 1 cut(s) 248
NmuCI GTSAC 1 cut(s) 209
NspI RCATGY 1 cut(s) 172
PctI GAATGC 1 cut(s) 154
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 248
SatI GCNGC 1 cut(s) 114
ScrFI CCNGG 1 cut(s) 229
SetI ASST 4 cut(s) 9, 15, 81, 277
SsiI CCGC 2 cut(s) 50, 302
StyD4I CCNGG 1 cut(s) 227
TaaI ACNGT 2 cut(s) 121, 264
TfiI GAWTC 1 cut(s) 183
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 2 cut(s) 17, 267
XceI RCATGY 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.