Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008881

Physical Location & Seq

Genomic Coordinates Reverse (-)

27386676 .. 27390167

Gene Structure 3492 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1170900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGAATGATCATCGCAAGTCAAATTACGTCGTGGAAATTACTTGGCTCCTGACATGGGAAAAGAAAATCATGAAGCTTCAGGTCTCCTTTTGCACGCTTATCTTCCTTTTCCTTATTCCGCCTTTCCCTTCCTCGGTTTTTTCTGACCTGAACTCGGATAGACAAGCCCTCCTCAACTTTGCTGCCACCGTTGTCCACATCAAAAAACTCAACTGGAATGTTTCCACACCAGTTTGCACGTCTTGGGTTGGAATCACATGCAATCTAAACAAAACGAGTGTGACTGCCATCCATCTTCCGGGGATTGGACTTTTCGGTTCCATCCCTTCCAACAGTATAGGGAAGCTTCATGCTCTTCAAGTCCTCAGCCTCCGCTCAAACTTCCTCTATGGAAGTCTGCCTTCTGATATCCTATCCATTCCTTCCTTGGAGTACCTTTACCTCCAACACAATAATTTCTCCGGCATGCTACCTGCCTCTCTGCCTCCCAACCTCGTTGTCTTGGACTTCTCCTTCAACTCCTTTTCCGGAAGCATTCCAACTAAAATCCAGAATCTTACACGCCTCACTACGTTGTACCTCCAAAACAATTTCATCTCTGGAGCCATCCCCAATCTGAACCTCCCCGCACTTAAGCTTTTGAATTTGAGCTACAATAACTTGAATGGCTCGGTTCCATATTCCCTCGAAAAATATTCGAACTCTTCATTTGTTGGGAATCCTCAGTTATGTGGAGAACCTCTCAACAATTGTTCTAAAACCACCTCCTCTACCCCTTCTGCATCCTCTGCTTTCTTGCCACCCTCGCCAACCATTCTGAAAAATCACCATGCTACACTCATAAAAAAACTCAGCCCGGGATCCATTATTGCCATGGCATTTGGGTGCTCTGCAGTGTTTATTCTTCTAGTTCTGGTGGTTGTCATATGCTGCTGGAAAAGGACTAACAAAGTAGGTGGCGGGATGTTGAAAGGGAAGGCTGCGGGTGATGGAAAGGGTGAGATGCCTAAGGATTTTGGGAGTGGAGTGCAAGAAGCTGAGAAGAACAAGCTGTTCTTCTTTGAAGGTTGCTATTTCAATTTTGATCTTGAGGATCTGCTGAGAGCGTCAGCTGAAGTTCTCGGCAAAGGAAGTTTCGGAACAACTTACAAAGCTGTATTGGATGAGGAAACAACGGTTGTGGTGAAGCGGTTGAGGGAAGTTGTGGTGGGGAAGAGGGAGTTTGAGCAGCATATGGAGCTGGTGGAGAAGGTAGGGAAGCACCCAAATGTCCTGCCTCCTCGCGCTTATTATTTTTCGAAAGATGAGAAGCTTTTGGTGTACAACTACATTCCAACAGGAAGCTTGTTTGCACACTTGCATGGAAGCAGTGATGCTGGGAGGTCTCCACTAGACTGGGATTCAAGAGTAAAGATTTCGCTCGGGATTGCCAAGGGACTTGCCCACATTCATTCCGAGGGTGCAAAATCTAGCCATGGCAATATAAAGTCCACAAACGTCCTCCTCACCCAAGAACTCGAGGCTTGCATCTCAGACGTAGGGTTGAGTCCTCTAATGAACTTCCCTCCAGCCATGTCCCGGGCCATGGGATATCGCGCTCCCGAGGCAACTGACATGCGGAAAATCACTCACAAATCTGATGTCTATAGCTTTGGTGTGCTCCTCTTAGAAATGCTGACAGGCAAAACCACGCTTCAATATCCAGGTTATGACTGCGTGGTTGATCTCCCGCGTTGGGTTAAGTCTGTTGTGCGTGAGGAGTGGACAGCTGAGGTTTTTGATTTGGAACTTCTAAAACAGCAACACAGTGAAGAAGAGATGGTGCAGATGCTGCAGATTGCTCTGGCATGCGTAACAAAATTGCCCGAAACACGCCCCAACATGGATGACGTAGTCAGAATGATAGAGGAACTCCGGCAATCCGACACCAAAACTAGGCAATCCTCCGAGTCGGAATTTAATGTGCAAACCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

659

Amino Acids

72.83

Weight (kDa)

8.29

Isoelectric Point (pI)

39.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	50 - 88	1.1e-07	Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr	PF07714	372 - 634	2.1e-33	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	372 - 632	6.6e-28	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008881)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g26210
malus_domestica	MD01G1170600.v1.1 MD01G1170700.v1.1 MD01G1170900.v1.1 MD07G1237300.v1.1
prunus_persica	Prupe.2G266400_v2.0.a1 Prupe.2G266400_v2.0.a1 Prupe.2G266400_v2.0.a1 Prupe.2G266400_v2.0.a1
pyrus_communis	pycom01g18300 pycom01g18310 pycom07g21350
rosa_chinensis	RchiOBHm_Chr1g0372661
rosa_laevigata	RLG00000026881
rosa_multiflora	Rmu_ssc0000419.1_g000004
rosa_roxburghii	Rroxscaffold_4G00284770 Rroxscaffold_4G00284780
rosa_rugosa	Rorug01G0373700
rosa_samantha	Rh1AG382200 Rh1BG345600 Rh1CG359000 Rh1DG376800
rosa_wichuraiana	Rw1G033620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	481
AccBSI	CCGCTC	1 cut(s)	375
AccII	CGCG	3 cut(s)	1284, 1596, 1732
AccIII	TCCGGA	1 cut(s)	527
AciI	CCGC	8 cut(s)	119, 373, 627, 960, 983, 1189, 1618, 1730
AclWI	GGATC	3 cut(s)	855, 868, 1101
AcsI	RAATTY	2 cut(s)	643, 1955
AcuI	CTGAAG	2 cut(s)	62, 1134
AfaI	GTAC	3 cut(s)	434, 578, 1322
AflII	CTTAAG	1 cut(s)	632
AgsI	TTSAA	9 cut(s)	359, 517, 643, 664, 970, 1064, 1078, 1404, 1697
AjiI	CACGTC	1 cut(s)	240
AjnI	CCWGG	1 cut(s)	1702
Alw21I	GWGCWC	2 cut(s)	890, 1662
Alw26I	GTCTC	2 cut(s)	88, 1389
AlwI	GGATC	3 cut(s)	855, 868, 1101
AlwNI	CAGNNNCTG	1 cut(s)	1831
Ama87I	CYCGRG	5 cut(s)	856, 1421, 1517, 1578, 1601
Aor13HI	TCCGGA	1 cut(s)	527
AoxI	GGCC	1 cut(s)	1581
ApeKI	GCWGC	5 cut(s)	182, 930, 980, 1228, 1831
ApoI	RAATTY	2 cut(s)	643, 1955
ArsI	GACNNNNNNTTYG	2 cut(s)	1626, 1658
AspLEI	GCGC	2 cut(s)	1286, 1598
AspS9I	GGNCC	1 cut(s)	1581
AsuC2I	CCSGG	5 cut(s)	300, 857, 858, 1579, 1580
AsuHPI	GGTGA	5 cut(s)	818, 998, 1010, 1195, 1498
AsuII	TTCGAA	2 cut(s)	698, 1298
AvaI	CYCGRG	5 cut(s)	856, 1421, 1517, 1578, 1601
AxyI	CCTNAGG	1 cut(s)	1008
BamHI	GGATCC	1 cut(s)	860
Bbv12I	GWGCWC	2 cut(s)	890, 1662
BbvCI	CCTCAGC	2 cut(s)	365, 1770
BbvI	GCAGC	5 cut(s)	169, 917, 967, 1240, 1818
BccI	CCATC	6 cut(s)	296, 300, 329, 614, 983, 1813
BciT130I	CCWGG	1 cut(s)	1704
BclI	TGATCA	1 cut(s)	7
BcnI	CCSGG	5 cut(s)	300, 857, 858, 1579, 1580
BcoDI	GTCTC	2 cut(s)	88, 1389
BfaI	CTAG	4 cut(s)	908, 1391, 1470, 1935
BfmI	CTRYAG	3 cut(s)	891, 1645, 1832
BfrI	CTTAAG	1 cut(s)	632
BfuAI	ACCTGC	1 cut(s)	481
BisI	GCNGC	5 cut(s)	183, 931, 981, 1229, 1832
BlsI	GCNGC	5 cut(s)	184, 932, 982, 1230, 1833
Bme1390I	CCNGG	6 cut(s)	300, 857, 858, 1579, 1580, 1704
BmeT110I	CYCGRG	5 cut(s)	856, 1421, 1517, 1578, 1601
BmgBI	CACGTC	1 cut(s)	240
BmgT120I	GGNCC	1 cut(s)	1581
BmiI	GGNNCC	5 cut(s)	47, 319, 604, 675, 862
BmrFI	CCNGG	6 cut(s)	300, 857, 858, 1579, 1580, 1704
BmrI	ACTGGG	1 cut(s)	1405
BmsI	GCATC	5 cut(s)	791, 993, 1363, 1536, 1818
BmuI	ACTGGG	1 cut(s)	1405
BplI	GAGNNNNNCTC	2 cut(s)	726, 758
BpmI	CTGGAG	2 cut(s)	621, 1551
Bpu10I	CCTNAGC	2 cut(s)	365, 1770
Bpu14I	TTCGAA	2 cut(s)	698, 1298
BpuEI	CTTGAG	1 cut(s)	1109
BpuMI	CCSGG	5 cut(s)	300, 857, 858, 1579, 1580
BsaI	GGTCTC	2 cut(s)	88, 1389
BsaWI	WCCGGW	1 cut(s)	527
BsaXI	ACNNNNNCTCC	2 cut(s)	153, 183
Bse1I	ACTGG	3 cut(s)	218, 230, 1400
Bse21I	CCTNAGG	1 cut(s)	1008
BseAI	TCCGGA	1 cut(s)	527
BseBI	CCWGG	1 cut(s)	1704
BseGI	GGATG	7 cut(s)	288, 321, 606, 782, 969, 1168, 1891
BseMII	CTCAG	7 cut(s)	379, 737, 865, 1029, 1091, 1545, 1761
BseNI	ACTGG	3 cut(s)	218, 230, 1400
BseRI	GAGGAG	6 cut(s)	161, 757, 1269, 1493, 1652, 1772
BseXI	GCAGC	5 cut(s)	169, 917, 967, 1240, 1818
BseYI	CCCAGC	1 cut(s)	1376
BsgI	GTGCAG	1 cut(s)	1844
Bsh1236I	CGCG	3 cut(s)	1284, 1596, 1732
BshFI	GGCC	1 cut(s)	1583
BsiHKAI	GWGCWC	2 cut(s)	890, 1662
BsiHKCI	CYCGRG	5 cut(s)	856, 1421, 1517, 1578, 1601
BsiSI	CCGG	6 cut(s)	299, 462, 528, 857, 1579, 1915
BslFI	GGGAC	2 cut(s)	1449, 1561
BsmAI	GTCTC	2 cut(s)	88, 1389
BsmFI	GGGAC	2 cut(s)	1449, 1561
BsmI	GAATGC	1 cut(s)	534
BsnI	GGCC	1 cut(s)	1583
Bso31I	GGTCTC	2 cut(s)	88, 1389
BsoBI	CYCGRG	5 cut(s)	856, 1421, 1517, 1578, 1601
Bsp119I	TTCGAA	2 cut(s)	698, 1298
Bsp1286I	GDGCHC	2 cut(s)	890, 1662
Bsp13I	TCCGGA	1 cut(s)	527
Bsp1407I	TGTACA	1 cut(s)	1320
Bsp143I	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
Bsp19I	CCATGG	3 cut(s)	873, 1474, 1584
BspACI	CCGC	8 cut(s)	119, 373, 627, 960, 983, 1189, 1618, 1730
BspANI	GGCC	1 cut(s)	1583
BspCNI	CTCAG	7 cut(s)	378, 736, 864, 1030, 1092, 1544, 1762
BspEI	TCCGGA	1 cut(s)	527
BspFNI	CGCG	3 cut(s)	1284, 1596, 1732
BspHI	TCATGA	1 cut(s)	69
BspLI	GGNNCC	5 cut(s)	47, 319, 604, 675, 862
BspMAI	CTGCAG	2 cut(s)	895, 1836
BspMI	ACCTGC	1 cut(s)	481
BspPI	GGATC	3 cut(s)	855, 868, 1101
BspQI	GCTCTTC	1 cut(s)	360
BspT104I	TTCGAA	2 cut(s)	698, 1298
BspTI	CTTAAG	1 cut(s)	632
BspTNI	GGTCTC	2 cut(s)	88, 1389
BsrBI	CCGCTC	1 cut(s)	375
BsrGI	TGTACA	1 cut(s)	1320
BsrI	ACTGG	3 cut(s)	218, 230, 1400
BssMI	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
BssT1I	CCWWGG	5 cut(s)	426, 873, 1431, 1474, 1584
Bst2UI	CCWGG	1 cut(s)	1704
Bst4CI	ACNGT	4 cut(s)	190, 335, 1177, 1808
Bst6I	CTCTTC	4 cut(s)	360, 709, 1208, 1809
BstAFI	CTTAAG	1 cut(s)	632
BstAPI	GCANNNNNTGC	2 cut(s)	788, 1831
BstAUI	TGTACA	1 cut(s)	1320
BstBI	TTCGAA	2 cut(s)	698, 1298
BstC8I	GCNNGC	4 cut(s)	95, 467, 1525, 1849
BstDEI	CTNAG	9 cut(s)	365, 723, 851, 1008, 1038, 1100, 1531, 1666, 1770
BstDSI	CCRYGG	3 cut(s)	873, 1474, 1584
BstF5I	GGATG	7 cut(s)	288, 321, 606, 782, 969, 1168, 1891
BstFNI	CGCG	3 cut(s)	1284, 1596, 1732
BstHHI	GCGC	2 cut(s)	1286, 1598
BstKTI	GATC	5 cut(s)	10, 863, 1087, 1096, 1726
BstMAI	GTCTC	2 cut(s)	88, 1389
BstMBI	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
BstMWI	GCNNNNNNNGC	5 cut(s)	788, 805, 1237, 1604, 1831
BstNI	CCWGG	1 cut(s)	1704
BstNSI	RCATGY	4 cut(s)	261, 469, 1618, 1851
BstSCI	CCNGG	6 cut(s)	298, 855, 856, 1577, 1578, 1702
BstSFI	CTRYAG	3 cut(s)	891, 1645, 1832
BstUI	CGCG	3 cut(s)	1284, 1596, 1732
BstV1I	GCAGC	5 cut(s)	169, 917, 967, 1240, 1818
BstX2I	RGATCY	2 cut(s)	860, 1093
BstXI	CCANNNNNNTGG	1 cut(s)	1395
BstYI	RGATCY	2 cut(s)	860, 1093
Bsu36I	CCTNAGG	1 cut(s)	1008
BsuRI	GGCC	1 cut(s)	1583
BtgI	CCRYGG	3 cut(s)	873, 1474, 1584
BtrI	CACGTC	1 cut(s)	240
BtsCI	GGATG	7 cut(s)	288, 321, 606, 782, 969, 1168, 1891
BtsI	GCAGTG	2 cut(s)	900, 1375
BtsIMutI	CAGTG	3 cut(s)	900, 1375, 1813
BveI	ACCTGC	1 cut(s)	481
Cac8I	GCNNGC	4 cut(s)	95, 467, 1525, 1849
CaiI	CAGNNNCTG	1 cut(s)	1831
CciI	TCATGA	1 cut(s)	69
CfoI	GCGC	2 cut(s)	1286, 1598
Cfr13I	GGNCC	1 cut(s)	1581
Cfr9I	CCCGGG	2 cut(s)	856, 1578
CseI	GACGC	1 cut(s)	1095
Csp6I	GTAC	3 cut(s)	433, 577, 1321
CspCI	CAANNNNNGTGG	4 cut(s)	937, 972, 1161, 1196
CviQI	GTAC	3 cut(s)	433, 577, 1321
DdeI	CTNAG	9 cut(s)	365, 723, 851, 1008, 1038, 1100, 1531, 1666, 1770
DpnI	GATC	5 cut(s)	9, 862, 1086, 1095, 1725
DpnII	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
Eam1104I	CTCTTC	4 cut(s)	360, 709, 1208, 1809
EarI	CTCTTC	4 cut(s)	360, 709, 1208, 1809
EciI	GGCGGA	1 cut(s)	108
Eco130I	CCWWGG	5 cut(s)	426, 873, 1431, 1474, 1584
Eco31I	GGTCTC	2 cut(s)	88, 1389
Eco32I	GATATC	2 cut(s)	409, 1592
Eco57I	CTGAAG	2 cut(s)	62, 1134
Eco81I	CCTNAGG	1 cut(s)	1008
Eco88I	CYCGRG	5 cut(s)	856, 1421, 1517, 1578, 1601
EcoRII	CCWGG	1 cut(s)	1702
EcoRV	GATATC	2 cut(s)	409, 1592
EcoT14I	CCWWGG	5 cut(s)	426, 873, 1431, 1474, 1584
ErhI	CCWWGG	5 cut(s)	426, 873, 1431, 1474, 1584
FalI	AAGNNNNNCTT	4 cut(s)	385, 417, 1040, 1072
FaqI	GGGAC	2 cut(s)	1449, 1561
FauI	CCCGC	4 cut(s)	634, 953, 976, 1737
FauNDI	CATATG	2 cut(s)	926, 1233
FbaI	TGATCA	1 cut(s)	7
Fnu4HI	GCNGC	5 cut(s)	183, 931, 981, 1229, 1832
FokI	GGATG	7 cut(s)	275, 308, 593, 769, 976, 1175, 1898
Fsp4HI	GCNGC	5 cut(s)	183, 931, 981, 1229, 1832
FspBI	CTAG	4 cut(s)	908, 1391, 1470, 1935
GlaI	GCGC	2 cut(s)	1285, 1597
GluI	GCNGC	5 cut(s)	183, 931, 981, 1229, 1832
GsaI	CCCAGC	1 cut(s)	1380
GsuI	CTGGAG	2 cut(s)	621, 1551
HaeIII	GGCC	1 cut(s)	1583
HapII	CCGG	6 cut(s)	299, 462, 528, 857, 1579, 1915
HgaI	GACGC	1 cut(s)	1095
HhaI	GCGC	2 cut(s)	1286, 1598
Hin6I	GCGC	2 cut(s)	1284, 1596
HinP1I	GCGC	2 cut(s)	1284, 1596
HindIII	AAGCTT	5 cut(s)	74, 344, 635, 1310, 1342
HinfI	GANTC	6 cut(s)	252, 553, 718, 1400, 1546, 1949
HpaII	CCGG	6 cut(s)	299, 462, 528, 857, 1579, 1915
HphI	GGTGA	5 cut(s)	818, 998, 1010, 1195, 1498
Hpy166II	GTNNAC	4 cut(s)	196, 1321, 1491, 1764
Hpy188III	TCNNGA	9 cut(s)	49, 70, 528, 550, 600, 1088, 1404, 1423, 1601
Hpy8I	GTNNAC	4 cut(s)	196, 1321, 1491, 1764
Hpy99I	CGWCG	1 cut(s)	32
HpyAV	CCTTC	9 cut(s)	138, 336, 411, 432, 523, 786, 970, 1058, 1243
HpyCH4III	ACNGT	4 cut(s)	190, 335, 1177, 1808
HpyCH4IV	ACGT	6 cut(s)	27, 239, 572, 1497, 1536, 1890
HpyF10VI	GCNNNNNNNGC	5 cut(s)	788, 805, 1237, 1604, 1831
HpyF3I	CTNAG	9 cut(s)	365, 723, 851, 1008, 1038, 1100, 1531, 1666, 1770
HpySE526I	ACGT	6 cut(s)	27, 239, 572, 1497, 1536, 1890
HspAI	GCGC	2 cut(s)	1284, 1596
Kpn2I	TCCGGA	1 cut(s)	527
Ksp22I	TGATCA	1 cut(s)	7
Kzo9I	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
LguI	GCTCTTC	1 cut(s)	360
LmnI	GCTCC	5 cut(s)	51, 602, 1237, 1603, 1665
Lsp1109I	GCAGC	5 cut(s)	169, 917, 967, 1240, 1818
LweI	GCATC	5 cut(s)	791, 993, 1363, 1536, 1818
MaeI	CTAG	4 cut(s)	908, 1391, 1470, 1935
MaeII	ACGT	6 cut(s)	27, 239, 572, 1497, 1536, 1890
MaeIII	GTNAC	2 cut(s)	280, 1852
MalI	GATC	5 cut(s)	9, 862, 1086, 1095, 1725
MbiI	CCGCTC	1 cut(s)	375
MboI	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
MfeI	CAATTG	1 cut(s)	748
MflI	RGATCY	2 cut(s)	860, 1093
MhlI	GDGCHC	2 cut(s)	890, 1662
MluCI	AATT	9 cut(s)	22, 36, 454, 589, 643, 748, 1078, 1859, 1955
MlyI	GAGTC	2 cut(s)	1555, 1958
MmeI	TCCRAC	7 cut(s)	229, 354, 469, 563, 1358, 1932, 1947
MroI	TCCGGA	1 cut(s)	527
MseI	TTAA	3 cut(s)	633, 1740, 1959
MslI	CAYNNNNRTG	3 cut(s)	883, 1266, 1359
MspA1I	CMGCKG	2 cut(s)	1112, 1769
MspCI	CTTAAG	1 cut(s)	632
MspI	CCGG	6 cut(s)	299, 462, 528, 857, 1579, 1915
MspR9I	CCNGG	6 cut(s)	300, 857, 858, 1579, 1580, 1704
MunI	CAATTG	1 cut(s)	748
Mva1269I	GAATGC	1 cut(s)	534
MvaI	CCWGG	1 cut(s)	1704
MvnI	CGCG	3 cut(s)	1284, 1596, 1732
MwoI	GCNNNNNNNGC	5 cut(s)	788, 805, 1237, 1604, 1831
NciI	CCSGG	5 cut(s)	300, 857, 858, 1579, 1580
NcoI	CCATGG	3 cut(s)	873, 1474, 1584
NdeI	CATATG	2 cut(s)	926, 1233
NdeII	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
NlaIV	GGNNCC	5 cut(s)	47, 319, 604, 675, 862
NmeAIII	GCCGAG	1 cut(s)	1101
NmuCI	GTSAC	1 cut(s)	280
NspI	RCATGY	4 cut(s)	261, 469, 1618, 1851
NspV	TTCGAA	2 cut(s)	698, 1298
PaeI	GCATGC	2 cut(s)	469, 1851
PaeR7I	CTCGAG	1 cut(s)	1517
PagI	TCATGA	1 cut(s)	69
PciSI	GCTCTTC	1 cut(s)	360
PctI	GAATGC	1 cut(s)	534
PfeI	GAWTC	4 cut(s)	252, 553, 718, 1400
PflFI	GACNNNGTC	1 cut(s)	1892
PkrI	GCNGC	5 cut(s)	184, 932, 982, 1230, 1833
PleI	GAGTC	2 cut(s)	1554, 1957
PpsI	GAGTC	2 cut(s)	1554, 1957
Psp6I	CCWGG	1 cut(s)	1702
PspFI	CCCAGC	1 cut(s)	1376
PspGI	CCWGG	1 cut(s)	1702
PspN4I	GGNNCC	5 cut(s)	47, 319, 604, 675, 862
PspPI	GGNCC	1 cut(s)	1581
PspXI	VCTCGAGB	1 cut(s)	1517
PstI	CTGCAG	2 cut(s)	895, 1836
PstNI	CAGNNNCTG	1 cut(s)	1831
PsuI	RGATCY	2 cut(s)	860, 1093
PsyI	GACNNNGTC	1 cut(s)	1892
PvuII	CAGCTG	2 cut(s)	1112, 1769
RsaI	GTAC	3 cut(s)	434, 578, 1322
RsaNI	GTAC	3 cut(s)	433, 577, 1321
RseI	CAYNNNNRTG	3 cut(s)	883, 1266, 1359
SapI	GCTCTTC	1 cut(s)	360
SaqAI	TTAA	3 cut(s)	633, 1740, 1959
SatI	GCNGC	5 cut(s)	183, 931, 981, 1229, 1832
Sau3AI	GATC	5 cut(s)	7, 860, 1084, 1093, 1723
Sau96I	GGNCC	1 cut(s)	1581
SchI	GAGTC	2 cut(s)	1555, 1958
ScrFI	CCNGG	6 cut(s)	300, 857, 858, 1579, 1580, 1704
SduI	GDGCHC	2 cut(s)	890, 1662
SfaNI	GCATC	5 cut(s)	791, 993, 1363, 1536, 1818
SfcI	CTRYAG	3 cut(s)	891, 1645, 1832
Sfr274I	CTCGAG	1 cut(s)	1517
SfuI	TTCGAA	2 cut(s)	698, 1298
SlaI	CTCGAG	1 cut(s)	1517
SmaI	CCCGGG	2 cut(s)	858, 1580
SmiMI	CAYNNNNRTG	3 cut(s)	883, 1266, 1359
SmlI	CTYRAG	3 cut(s)	632, 1088, 1517
SmoI	CTYRAG	3 cut(s)	632, 1088, 1517
SphI	GCATGC	2 cut(s)	469, 1851
Sse9I	AATT	9 cut(s)	22, 36, 454, 589, 643, 748, 1078, 1859, 1955
SsiI	CCGC	8 cut(s)	119, 373, 627, 960, 983, 1189, 1618, 1730
SspI	AATATT	1 cut(s)	695
SspMI	CTAG	4 cut(s)	908, 1391, 1470, 1935
StyD4I	CCNGG	6 cut(s)	298, 855, 856, 1577, 1578, 1702
StyI	CCWWGG	5 cut(s)	426, 873, 1431, 1474, 1584
TaaI	ACNGT	4 cut(s)	190, 335, 1177, 1808
TaiI	ACGT	6 cut(s)	30, 242, 575, 1500, 1539, 1893
TaqI	TCGA	4 cut(s)	687, 698, 1298, 1518
TasI	AATT	9 cut(s)	22, 36, 454, 589, 643, 748, 1078, 1859, 1955
TatI	WGTACW	1 cut(s)	1320
TfiI	GAWTC	4 cut(s)	252, 553, 718, 1400
Tru1I	TTAA	3 cut(s)	633, 1740, 1959
Tru9I	TTAA	3 cut(s)	633, 1740, 1959
TscAI	CASTG	3 cut(s)	900, 1375, 1813
TseFI	GTSAC	1 cut(s)	280
TseI	GCWGC	5 cut(s)	182, 930, 980, 1228, 1831
Tsp45I	GTSAC	1 cut(s)	280
TspDTI	ATGAA	7 cut(s)	17, 86, 338, 583, 696, 1439, 1571
TspMI	CCCGGG	2 cut(s)	856, 1578
TspRI	CASTG	3 cut(s)	900, 1375, 1813
Tth111I	GACNNNGTC	1 cut(s)	1892
Vha464I	CTTAAG	1 cut(s)	632
XapI	RAATTY	2 cut(s)	643, 1955
XceI	RCATGY	4 cut(s)	261, 469, 1618, 1851
XcmI	CCANNNNNNNNNTGG	2 cut(s)	424, 871
XhoI	CTCGAG	1 cut(s)	1517
XmaI	CCCGGG	2 cut(s)	856, 1578
XspI	CTAG	4 cut(s)	908, 1391, 1470, 1935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.