MD01G1174500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016397
Physical Location & Seq
Forward (+)
27560103 .. 27561058
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1174500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCTTCAATTGCCATTTCTGCTTCACTGCAAACAGTTTGCAGCTCACATCAAGTCACAAAACAGCCCCAATCGAAACCAGGAGCTCGCTCTTTGGGGACAAAAGAAGCAACGCATGTTGTTGCTCTGAATGTGGTAGCAAATAGTTTGAACGTATCTGCACAAGAGGAATCTGCTTTACGCGTCGAAAAACACAAGGCAGAGGATGCTGCTGACTCCAAATCAGAGGATGGTCTCGGGGGCGCAGAGTTGTCTGTCTTGAAATTCAGGGACGAAAGGTGGAAGAATGGGACATGGGATCTGAATATGTTTTCCAAAAATGGCAAGATGGATTGGGATGGCATGATTCTAGCAGAAGCTAAAAGGAGGAAGTTCCTTGAGCTTCACCCGGAAGCATCTACGAACGATGACCCGGTAGTGTTTAGGAGCTCCATCATACCATGGTGGGCATGGATGATGAGATCTTATCTCCCGGAGGCTGAGCTGATCAACGGTCGAGCGGCAATGGTGGGATTCTTCATGGCATATGTTGTAGATGCCTTGACGGGGATTGGGGTTGTCGGGCAAACAGGAAACTTCATCTGCAAGGCAGGGTTTTTCGTGACAGTCACTGCTATAATACTCATTAGGCGAACACAAGATCTCGGAAACCTTAGGAAGCTCGCCGATGAAGCTACTGTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroa_b-bind

Protein Analysis

229

Amino Acids

25.02

Weight (kDa)

6.45

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016397)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26541
malus_domestica MD01G1174500.v1.1
prunus_persica Prupe.2G269900_v2.0.a1
pyrus_communis pycom01g18620
rosa_chinensis RchiOBHm_Chr1g0373221
rosa_laevigata RLG00000026842
rosa_multiflora Rmu_sc0000170.1_g000010 Rmu_sc0003895.1_g000001
rosa_roxburghii Rroxscaffold_4G00284380
rosa_rugosa Rorug01G0376700
rosa_samantha Rh1AG385400 Rh1BG349400 Rh1CG362700 Rh1DG380300
rosa_wichuraiana Rw1G034000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 500
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 500
AclWI GGATC 1 cut(s) 306
AcsI RAATTY 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 546
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 4 cut(s) 9, 151, 262, 683
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 8 cut(s) 45, 86, 359, 382, 429, 484, 661, 674
AluI AGCT 8 cut(s) 45, 86, 359, 382, 429, 484, 661, 674
Alw21I GWGCWC 2 cut(s) 88, 431
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 306
AlwNI CAGNNNCTG 1 cut(s) 611
Ama87I CYCGRG 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 42, 209
ApoI RAATTY 1 cut(s) 263
AspLEI GCGC 1 cut(s) 245
AsuC2I CCSGG 3 cut(s) 389, 413, 473
AsuHPI GGTGA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 236
AxyI CCTNAGG 1 cut(s) 653
BanII GRGCYC 2 cut(s) 88, 431
Bbv12I GWGCWC 2 cut(s) 88, 431
BbvI GCAGC 2 cut(s) 54, 196
BccI CCATC 4 cut(s) 224, 322, 332, 440
BciT130I CCWGG 1 cut(s) 81
BclI TGATCA 1 cut(s) 486
BcnI CCSGG 3 cut(s) 389, 413, 473
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 1 cut(s) 350
BglII AGATCT 2 cut(s) 461, 640
BisI GCNGC 3 cut(s) 43, 210, 501
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 3 cut(s) 44, 211, 502
Bme1390I CCNGG 4 cut(s) 81, 389, 413, 473
BmeT110I CYCGRG 1 cut(s) 236
BmrFI CCNGG 4 cut(s) 81, 389, 413, 473
BmsI GCATC 3 cut(s) 196, 404, 526
Bpu1102I GCTNAGC 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 3 cut(s) 389, 413, 473
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 440
Bsc4I CCNNNNNNNGG 1 cut(s) 546
Bse21I CCTNAGG 1 cut(s) 653
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 440
BseGI GGATG 4 cut(s) 211, 235, 343, 459
BseLI CCNNNNNNNGG 1 cut(s) 546
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 471
BseXI GCAGC 2 cut(s) 54, 196
BsgI GTGCAG 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 496
BsiHKAI GWGCWC 2 cut(s) 88, 431
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 3 cut(s) 389, 413, 473
BslFI GGGAC 3 cut(s) 112, 284, 304
BslI CCNNNNNNNGG 1 cut(s) 546
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 3 cut(s) 112, 284, 304
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 88, 431
Bsp143I GATC 4 cut(s) 298, 461, 486, 640
Bsp1720I GCTNAGC 1 cut(s) 480
Bsp19I CCATGG 1 cut(s) 440
BspACI CCGC 1 cut(s) 500
BspCNI CTCAG 1 cut(s) 472
BspFNI CGCG 1 cut(s) 183
BspPI GGATC 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 440
BssMI GATC 4 cut(s) 298, 461, 486, 640
BssT1I CCWWGG 1 cut(s) 440
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 4 cut(s) 37, 494, 607, 679
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 2 cut(s) 88, 663
BstDEI CTNAG 2 cut(s) 480, 653
BstDSI CCRYGG 1 cut(s) 440
BstF5I GGATG 4 cut(s) 211, 235, 343, 459
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 4 cut(s) 301, 464, 489, 643
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 4 cut(s) 298, 461, 486, 640
BstMCI CGRYCG 1 cut(s) 496
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 206, 671
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 4 cut(s) 79, 387, 411, 471
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 54, 196
BstX2I RGATCY 3 cut(s) 298, 461, 640
BstYI RGATCY 3 cut(s) 298, 461, 640
Bsu36I CCTNAGG 1 cut(s) 653
BtgI CCRYGG 1 cut(s) 440
BtsCI GGATG 4 cut(s) 211, 235, 343, 459
BtsI GCAGTG 2 cut(s) 26, 609
BtsIMutI CAGTG 2 cut(s) 26, 609
Cac8I GCNNGC 2 cut(s) 88, 663
CaiI CAGNNNCTG 1 cut(s) 611
CfoI GCGC 1 cut(s) 245
CseI GACGC 1 cut(s) 172
CviAII CATG 6 cut(s) 116, 294, 343, 441, 450, 520
DdeI CTNAG 2 cut(s) 480, 653
DpnI GATC 4 cut(s) 300, 463, 488, 642
DpnII GATC 4 cut(s) 298, 461, 486, 640
Ecl136II GAGCTC 2 cut(s) 86, 429
Eco130I CCWWGG 1 cut(s) 440
Eco24I GRGCYC 2 cut(s) 88, 431
Eco31I GGTCTC 1 cut(s) 239
Eco53kI GAGCTC 2 cut(s) 86, 429
Eco81I CCTNAGG 1 cut(s) 653
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 2 cut(s) 86, 429
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 440
EcoT38I GRGCYC 2 cut(s) 88, 431
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 6 cut(s) 119, 297, 346, 444, 453, 523
FaqI GGGAC 3 cut(s) 112, 284, 304
FatI CATG 6 cut(s) 115, 293, 342, 440, 449, 519
FauNDI CATATG 1 cut(s) 526
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 3 cut(s) 43, 210, 501
FokI GGATG 4 cut(s) 218, 242, 350, 466
FriOI GRGCYC 2 cut(s) 88, 431
Fsp4HI GCNGC 3 cut(s) 43, 210, 501
FspBI CTAG 1 cut(s) 350
GlaI GCGC 1 cut(s) 244
GluI GCNGC 3 cut(s) 43, 210, 501
HapII CCGG 3 cut(s) 389, 413, 473
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 245
Hin1II CATG 6 cut(s) 119, 297, 346, 444, 453, 523
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 4 cut(s) 170, 215, 346, 513
HpaII CCGG 3 cut(s) 389, 413, 473
HphI GGTGA 1 cut(s) 377
Hpy188I TCNGA 4 cut(s) 129, 226, 303, 647
Hpy188III TCNNGA 2 cut(s) 259, 601
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 4 cut(s) 37, 494, 607, 679
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 4 cut(s) 31, 42, 161, 585
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 206, 671
HpyF3I CTNAG 2 cut(s) 480, 653
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 6 cut(s) 119, 297, 346, 444, 453, 523
HspAI GCGC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 4 cut(s) 298, 461, 486, 640
LmnI GCTCC 3 cut(s) 83, 426, 434
LpnPI CCDG 8 cut(s) 66, 93, 253, 402, 426, 486, 555, 576
Lsp1109I GCAGC 2 cut(s) 54, 196
LweI GCATC 3 cut(s) 196, 404, 526
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 55, 601, 607
MalI GATC 4 cut(s) 300, 463, 488, 642
MbiI CCGCTC 1 cut(s) 500
MboI GATC 4 cut(s) 298, 461, 486, 640
MboII GAAGA 2 cut(s) 295, 508
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 3 cut(s) 298, 461, 640
MhlI GDGCHC 2 cut(s) 88, 431
MluCI AATT 2 cut(s) 9, 263
MluI ACGCGT 1 cut(s) 181
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 5 cut(s) 160, 196, 220, 360, 469
MspI CCGG 3 cut(s) 389, 413, 473
MspR9I CCNGG 4 cut(s) 81, 389, 413, 473
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 206, 671
NciI CCSGG 3 cut(s) 389, 413, 473
NcoI CCATGG 1 cut(s) 440
NdeI CATATG 1 cut(s) 526
NdeII GATC 4 cut(s) 298, 461, 486, 640
NlaIII CATG 6 cut(s) 119, 297, 346, 444, 453, 523
NmuCI GTSAC 3 cut(s) 55, 601, 607
NspI RCATGY 1 cut(s) 119
PfeI GAWTC 3 cut(s) 170, 346, 513
PfoI TCCNGGA 1 cut(s) 471
PkrI GCNGC 3 cut(s) 44, 211, 502
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
Psp124BI GAGCTC 2 cut(s) 88, 431
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 611
PsuI RGATCY 3 cut(s) 298, 461, 640
SacI GAGCTC 2 cut(s) 88, 431
SatI GCNGC 3 cut(s) 43, 210, 501
Sau3AI GATC 4 cut(s) 298, 461, 486, 640
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 4 cut(s) 81, 389, 413, 473
SduI GDGCHC 2 cut(s) 88, 431
SfaNI GCATC 3 cut(s) 196, 404, 526
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 2 cut(s) 9, 263
SsiI CCGC 1 cut(s) 500
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 2 cut(s) 88, 431
StyD4I CCNGG 4 cut(s) 79, 387, 411, 471
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 4 cut(s) 37, 494, 607, 679
TaiI ACGT 1 cut(s) 156
TaqI TCGA 3 cut(s) 74, 186, 496
TasI AATT 2 cut(s) 9, 263
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 3 cut(s) 170, 346, 513
TscAI CASTG 2 cut(s) 33, 616
TseFI GTSAC 3 cut(s) 55, 601, 607
TseI GCWGC 2 cut(s) 42, 209
Tsp45I GTSAC 3 cut(s) 55, 601, 607
TspDTI ATGAA 3 cut(s) 508, 568, 684
TspRI CASTG 2 cut(s) 33, 616
XapI RAATTY 1 cut(s) 263
XceI RCATGY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 447
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.