MD01G1194500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013720
Physical Location & Seq
Forward (+)
29328005 .. 29329218
1214 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1194500.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGTGTGCGTATTGTGGTGGTTTGGGTTTTGCGCAGAAACTGTTTGATGGAATGTGGGAGAGAGATTTAGTCTCTTGGAATTCTTTGATATGTGGATATAGTCAGTGCAATCGGTTTAATGAGGTTTTGGGTCTATTTGAGGCAATACAGGCAGCGAATGTGAGGGCTGATGCGGTGACGATGGTGAAAGTTATTTTGGCATGTAATTACTTGGGTGAACGGAAATTTGCTGATTCTGTGGTTAAGCGTGATTTGGCGAATTTGGCTCGGGAAGTATTTGATCGGATGGGTGAAAGAAATATAGTTTCTTGGAATGCCATGATCACAGGATATGCGAAAGTGGGAAACTTTGTTGATGCAAGAAAGCTTTTCAATGAGATGCCGAAGAGGAATGTGGTGTCTTGGACTTCCATGATCACTTGTTACTCTCAAGCTAACCAACATTCTGAGGCGGTGAGTCTTTTTCAAGAAATGATGGCAGCTAATGTGAGACCGGATGAAATAGCCGTGGCTAGTGTGCTTTCTGCTTGTGCCCATGTTGGTTCACTTGATGTGGGAGAGACTGTCCACCACTACATAAAAACGCATGGTGTGAAAGTAGATGTTTATGTCGGAAACGCTTTGATAGATATGTATTGCAAATGTGGGGTGGTTGAGAAGGCAATGGAAGTGTTCCAATGGATGGAAAAGAAGGACTCTGTCTCATGGACTTCGGTAATTTCAGGCCTTGCTGTCAACGGTTTTGCAGATTCTGCACTCGAATGTTTCTCGCGGATGTTGAGAGAAGGTGTTCAACCAACTCATGGAACGTTTTTTGAAAGCATGGAGAAGATTCATGGACTGATGCCAGAATTGAAACACTATGGATGTGTTGTGGATCTTTTGAGCCGCTCTGGCAAACTAGACAAAGCATCTGAGTTTGTACAAACTATGCCTGTTGGACCAGGTGTTGTAGTATGGAGGATATTGCTGAGTGCATGCAAGATTCACGGGAATGCGGCCCTAGCTGAGATTGTCACAGCCAAGCTTGTAGAACTGGATCCTTCCAACAGCGGAATTACATTCTCTCATCGAGTACTTACTCAGGTTCAGATAGATGGGATGATGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

371

Amino Acids

40.89

Weight (kDa)

6.22

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 22 - 64 3.6e-06 PPR repeat family
PPR_long PF17177 41 - 184 2.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 98 - 127 8.4e-06 PPR repeat
PPR_2 PF13041 99 - 132 9.6e-10 PPR repeat family
PPR PF01535 102 - 132 4.5e-09 PPR repeat
PPR_2 PF13041 130 - 179 1.5e-10 PPR repeat family
PPR PF01535 133 - 161 1e-07 PPR repeat
TPR_24 PF23276 169 - 232 6.6e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 197 - 229 4.1e-06 PPR repeat
PPR_2 PF13041 201 - 231 1.3e-06 PPR repeat family
PPR_2 PF13041 231 - 271 4.9e-06 PPR repeat family
PPR PF01535 234 - 264 4.4e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 33
AccB7I CCANNNNNTGG 2 cut(s) 682, 803
AccBSI CCGCTC 1 cut(s) 891
AccII CGCG 1 cut(s) 772
AciI CCGC 6 cut(s) 173, 452, 772, 889, 998, 1053
AclI AACGTT 1 cut(s) 809
AclWI GGATC 3 cut(s) 885, 1034, 1047
AcsI RAATTY 3 cut(s) 79, 224, 259
AfaI GTAC 2 cut(s) 924, 1077
AfiI CCNNNNNNNGG 2 cut(s) 682, 803
AgsI TTSAA 5 cut(s) 373, 467, 794, 818, 856
AjnI CCWGG 1 cut(s) 943
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 367, 434, 482, 1007, 1027
AluI AGCT 5 cut(s) 367, 434, 482, 1007, 1027
Alw26I GTCTC 4 cut(s) 76, 484, 554, 706
AlwI GGATC 3 cut(s) 885, 1034, 1047
AlwNI CAGNNNCTG 2 cut(s) 40, 752
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 2 cut(s) 724, 999
ApeKI GCWGC 2 cut(s) 152, 479
ApoI RAATTY 3 cut(s) 79, 224, 259
Asp700I GAANNNNTTC 1 cut(s) 789
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 941, 1000
AsuHPI GGTGA 5 cut(s) 187, 196, 227, 302, 466
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 1 cut(s) 941
BaeGI GKGCMC 1 cut(s) 535
BamHI GGATCC 1 cut(s) 1039
BbvI GCAGC 2 cut(s) 164, 491
BccI CCATC 6 cut(s) 41, 175, 280, 469, 676, 1091
BceAI ACGGC 1 cut(s) 491
BciT130I CCWGG 1 cut(s) 945
BclI TGATCA 2 cut(s) 321, 414
BcoDI GTCTC 4 cut(s) 76, 484, 554, 706
BfaI CTAG 3 cut(s) 513, 902, 1004
BglI GCCNNNNNGGC 1 cut(s) 894
BisI GCNGC 4 cut(s) 153, 480, 889, 999
BlsI GCNGC 4 cut(s) 154, 481, 890, 1000
BmcAI AGTACT 1 cut(s) 1077
Bme1390I CCNGG 1 cut(s) 945
Bme18I GGWCC 1 cut(s) 941
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 941, 1000
BmiI GGNNCC 1 cut(s) 1041
BmrFI CCNGG 1 cut(s) 945
BmsI GCATC 6 cut(s) 160, 346, 369, 834, 920, 1095
BpuEI CTTGAG 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 682, 803
Bse1I ACTGG 1 cut(s) 1041
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 945
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 6 cut(s) 291, 502, 687, 780, 872, 1107
BseLI CCNNNNNNNGG 2 cut(s) 682, 803
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 5 cut(s) 438, 906, 962, 999, 1097
BseNI ACTGG 1 cut(s) 1041
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 2 cut(s) 164, 491
BsgI GTGCAG 1 cut(s) 738
Bsh1236I CGCG 1 cut(s) 772
BshFI GGCC 2 cut(s) 726, 1001
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 1 cut(s) 494
BslI CCNNNNNNNGG 2 cut(s) 682, 803
BsmAI GTCTC 4 cut(s) 76, 484, 554, 706
BsmI GAATGC 2 cut(s) 319, 1000
BsnI GGCC 2 cut(s) 726, 1001
Bso31I GGTCTC 1 cut(s) 484
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 535
Bsp1407I TGTACA 1 cut(s) 922
Bsp143I GATC 5 cut(s) 280, 321, 414, 877, 1039
BspACI CCGC 6 cut(s) 173, 452, 772, 889, 998, 1053
BspANI GGCC 2 cut(s) 726, 1001
BspCNI CTCAG 5 cut(s) 439, 907, 963, 1000, 1096
BspFNI CGCG 1 cut(s) 772
BspLI GGNNCC 1 cut(s) 1041
BspPI GGATC 3 cut(s) 885, 1034, 1047
BspTNI GGTCTC 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 891
BsrDI GCAATG 1 cut(s) 669
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 1 cut(s) 1041
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 5 cut(s) 280, 321, 414, 877, 1039
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 3 cut(s) 42, 565, 740
Bst6I CTCTTC 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 922
BstC8I GCNNGC 1 cut(s) 979
BstDEI CTNAG 5 cut(s) 447, 915, 971, 1008, 1083
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 6 cut(s) 291, 502, 687, 780, 872, 1107
BstFNI CGCG 1 cut(s) 772
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 5 cut(s) 283, 324, 417, 880, 1042
BstMAI GTCTC 4 cut(s) 76, 484, 554, 706
BstMBI GATC 5 cut(s) 280, 321, 414, 877, 1039
BstMWI GCNNNNNNNGC 5 cut(s) 149, 263, 752, 894, 1004
BstNI CCWGG 1 cut(s) 945
BstNSI RCATGY 2 cut(s) 204, 981
BstSCI CCNGG 1 cut(s) 943
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 1 cut(s) 772
BstV1I GCAGC 2 cut(s) 164, 491
BstX2I RGATCY 2 cut(s) 877, 1039
BstYI RGATCY 2 cut(s) 877, 1039
BsuRI GGCC 2 cut(s) 726, 1001
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 6 cut(s) 291, 502, 687, 780, 872, 1107
BtsIMutI CAGTG 1 cut(s) 110
Cac8I GCNNGC 1 cut(s) 979
CaiI CAGNNNCTG 2 cut(s) 40, 752
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 941, 1000
CsiI ACCWGGT 1 cut(s) 943
Csp6I GTAC 2 cut(s) 923, 1076
CviQI GTAC 2 cut(s) 923, 1076
DdeI CTNAG 5 cut(s) 447, 915, 971, 1008, 1083
DpnI GATC 5 cut(s) 282, 323, 416, 879, 1041
DpnII GATC 5 cut(s) 280, 321, 414, 877, 1039
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Eco147I AGGCCT 1 cut(s) 726
Eco31I GGTCTC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 943
FbaI TGATCA 2 cut(s) 321, 414
Fnu4HI GCNGC 4 cut(s) 153, 480, 889, 999
FokI GGATG 5 cut(s) 298, 509, 694, 787, 879
Fsp4HI GCNGC 4 cut(s) 153, 480, 889, 999
FspBI CTAG 3 cut(s) 513, 902, 1004
FspI TGCGCA 1 cut(s) 33
GlaI GCGC 1 cut(s) 33
GluI GCNGC 4 cut(s) 153, 480, 889, 999
HaeIII GGCC 2 cut(s) 726, 1001
HapII CCGG 1 cut(s) 494
HhaI GCGC 1 cut(s) 34
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 2 cut(s) 365, 1025
HinfI GANTC 6 cut(s) 233, 457, 695, 749, 832, 985
HpaII CCGG 1 cut(s) 494
HphI GGTGA 5 cut(s) 187, 196, 227, 302, 466
Hpy166II GTNNAC 4 cut(s) 218, 545, 568, 736
Hpy188I TCNGA 5 cut(s) 285, 448, 614, 916, 1092
Hpy188III TCNNGA 2 cut(s) 269, 467
Hpy8I GTNNAC 4 cut(s) 218, 545, 568, 736
HpyAV CCTTC 4 cut(s) 652, 685, 779, 1053
HpyCH4III ACNGT 3 cut(s) 42, 565, 740
HpyCH4IV ACGT 1 cut(s) 809
HpyCH4V TGCA 7 cut(s) 108, 359, 639, 746, 755, 977, 981
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 263, 752, 894, 1004
HpyF3I CTNAG 5 cut(s) 447, 915, 971, 1008, 1083
HpySE526I ACGT 1 cut(s) 809
HspAI GCGC 1 cut(s) 32
Ksp22I TGATCA 2 cut(s) 321, 414
Kzo9I GATC 5 cut(s) 280, 321, 414, 877, 1039
Lsp1109I GCAGC 2 cut(s) 164, 491
LweI GCATC 6 cut(s) 160, 346, 369, 834, 920, 1095
MabI ACCWGGT 1 cut(s) 943
MaeI CTAG 3 cut(s) 513, 902, 1004
MaeII ACGT 1 cut(s) 809
MaeIII GTNAC 3 cut(s) 175, 422, 1015
MalI GATC 5 cut(s) 282, 323, 416, 879, 1041
MbiI CCGCTC 1 cut(s) 891
MboI GATC 5 cut(s) 280, 321, 414, 877, 1039
MboII GAAGA 2 cut(s) 397, 841
MflI RGATCY 2 cut(s) 877, 1039
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 7 cut(s) 79, 205, 224, 259, 717, 851, 1056
MlyI GAGTC 2 cut(s) 466, 689
MmeI TCCRAC 3 cut(s) 592, 919, 1071
MnlI CCTC 6 cut(s) 115, 133, 156, 381, 442, 954
MroXI GAANNNNTTC 1 cut(s) 789
MseI TTAA 2 cut(s) 117, 243
MslI CAYNNNNRTG 2 cut(s) 760, 993
MspA1I CMGCKG 1 cut(s) 1053
MspI CCGG 1 cut(s) 494
MspR9I CCNGG 1 cut(s) 945
Mva1269I GAATGC 2 cut(s) 319, 1000
MvaI CCWGG 1 cut(s) 945
MvnI CGCG 1 cut(s) 772
MwoI GCNNNNNNNGC 5 cut(s) 149, 263, 752, 894, 1004
NdeII GATC 5 cut(s) 280, 321, 414, 877, 1039
NlaIV GGNNCC 1 cut(s) 1041
NmuCI GTSAC 2 cut(s) 175, 1015
NsbI TGCGCA 1 cut(s) 33
NspI RCATGY 2 cut(s) 204, 981
PaeI GCATGC 1 cut(s) 981
PceI AGGCCT 1 cut(s) 726
PctI GAATGC 2 cut(s) 319, 1000
PdmI GAANNNNTTC 1 cut(s) 789
PfeI GAWTC 4 cut(s) 233, 749, 832, 985
PflFI GACNNNGTC 1 cut(s) 698
PflMI CCANNNNNTGG 2 cut(s) 682, 803
PkrI GCNGC 4 cut(s) 154, 481, 890, 1000
PleI GAGTC 2 cut(s) 465, 689
PpsI GAGTC 2 cut(s) 465, 689
Psp1406I AACGTT 1 cut(s) 809
Psp6I CCWGG 1 cut(s) 943
PspGI CCWGG 1 cut(s) 943
PspN4I GGNNCC 1 cut(s) 1041
PspPI GGNCC 2 cut(s) 941, 1000
PstNI CAGNNNCTG 2 cut(s) 40, 752
PsuI RGATCY 2 cut(s) 877, 1039
PsyI GACNNNGTC 1 cut(s) 698
RsaI GTAC 2 cut(s) 924, 1077
RsaNI GTAC 2 cut(s) 923, 1076
RseI CAYNNNNRTG 2 cut(s) 760, 993
SaqAI TTAA 2 cut(s) 117, 243
SatI GCNGC 4 cut(s) 153, 480, 889, 999
Sau3AI GATC 5 cut(s) 280, 321, 414, 877, 1039
Sau96I GGNCC 2 cut(s) 941, 1000
ScaI AGTACT 1 cut(s) 1077
SchI GAGTC 2 cut(s) 466, 689
ScrFI CCNGG 1 cut(s) 945
SduI GDGCHC 1 cut(s) 535
SexAI ACCWGGT 1 cut(s) 943
SfaNI GCATC 6 cut(s) 160, 346, 369, 834, 920, 1095
SinI GGWCC 1 cut(s) 941
SmiMI CAYNNNNRTG 2 cut(s) 760, 993
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SphI GCATGC 1 cut(s) 981
Sse9I AATT 7 cut(s) 79, 205, 224, 259, 717, 851, 1056
SseBI AGGCCT 1 cut(s) 726
SsiI CCGC 6 cut(s) 173, 452, 772, 889, 998, 1053
SspMI CTAG 3 cut(s) 513, 902, 1004
StuI AGGCCT 1 cut(s) 726
StyD4I CCNGG 1 cut(s) 943
TaaI ACNGT 3 cut(s) 42, 565, 740
TaiI ACGT 1 cut(s) 812
TaqI TCGA 2 cut(s) 759, 1072
TasI AATT 7 cut(s) 79, 205, 224, 259, 717, 851, 1056
TatI WGTACW 2 cut(s) 922, 1075
TauI GCSGC 2 cut(s) 891, 1001
TfiI GAWTC 4 cut(s) 233, 749, 832, 985
Tru1I TTAA 2 cut(s) 117, 243
Tru9I TTAA 2 cut(s) 117, 243
TscAI CASTG 1 cut(s) 110
TseFI GTSAC 2 cut(s) 175, 1015
TseI GCWGC 2 cut(s) 152, 479
Tsp45I GTSAC 2 cut(s) 175, 1015
TspDTI ATGAA 2 cut(s) 513, 824
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 1 cut(s) 110
Tth111I GACNNNGTC 1 cut(s) 698
Van91I CCANNNNNTGG 2 cut(s) 682, 803
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 3 cut(s) 79, 224, 259
XceI RCATGY 2 cut(s) 204, 981
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 3 cut(s) 513, 902, 1004
ZrmI AGTACT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.