Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014091

Physical Location & Seq

Genomic Coordinates Forward (+)

29682612 .. 29683823

Gene Structure 1212 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1199700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGAAAAGCTTCTCCTCAGGATTCCATTTCCCAGACCAACCACGAATTTCAGGAGAAAGAGAAAGCACAGAGGAAGGTAAATTACTACAACAAGAAGTAGAGAAAAGATCAGAGTTCAACAATTGTGAATGGGATTTCAGTCTCTCGACAGTTGTGTCCTCCAACGACTGCCGCGGCGCCGCATCCAACACCCTCGGTGTAATCGAGTTCAACCCATCAAACACCGTCTTAGCCACCGGCGGAATCGCCAGAAAAATCAGAGTTTACAGCCTGAAATCTCTATTGCCCAATTACGATAATCAAACTTGTGGAAGTTCAAGTGATACTATGGCGGATTTGATAGACCACGTAGATGCATGCGACTACACCATTTGCACTCCCGCCAAGCTCAGCAGCCTCCGCTGGCGGCCCGGATCCGACGGCCGGGTCATGGCCTCCGGCGACTACGACGGCGTCGTCACGGAATACGACATCAAAACAAAACTACCCATCTTCGAGCGCGACGAGCACGGCGGGCGCAGAGTCTGGAGCGTGGATTATTCGCATTCTGATCCGGTTCTCGGAGCGTCTGGCTCCGACGATGGGACACTACAGATGTGGGACCCGCGCTGCGAGGGCGGGGAATGCGTGGCGAGTGTGCAGCCCAGTGACGCGCGAAGTCCGGTCTGCTGCGTGGAGTTTGATCCGTTCGGCGGCGCGCTTCTGGCCGTTGGATGCGCGGACCGGAAGGCCTACGCCTACGATGTTCGGAAAGTGGCGGGCCCGGTTATGGTCTTCGACGGACACAGGAAAACCGTGACGTACGTGCGGTTTCTCGACAGCCGTACGGTGGTGACAGCTGCAACGGACGGGTGTTTGAAGCTGTGGGACACGGAGGATTCGCGGGCGATTGAGACGTACAAGGGGCACGCGAACAGTCGGAGCTTTGTTGGGTTATCGGTGTGGAGAAACGGAGGGCTAATTGGATGCGGGTCGGAAAATAACCGGCTTTTCGTGTACGATAAGAGGTGGGGCGAGCCCATTTGGGTCCACGAGTTTGGGGCGGTGGCTGCCGGTGGCAGTAGTGGTGGGTGCGGTGAGGAGGAAGGCGCGTTTGTGAGTAGCGTGTGCTGGCGACAAGTTGGTGAGGAGCAGTGCACGCTGGTGGCGGGTGGATCAGATGGGGTTTTGCAGGTTTTTCTGGGGAGGAGGAAGTCACTCACGTCGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009657 GO:0009658 GO:0009791 GO:0009812 GO:0009987 GO:0010114 GO:0010119 GO:0010218 GO:0010224 GO:0010228 GO:0016043 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043393 GO:0043412 GO:0043496 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051098 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40

Protein Analysis

404

Amino Acids

43.82

Weight (kDa)

5.07

Isoelectric Point (pI)

47.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	67 - 335	2.5e-23	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	119 - 271	2.2e-10	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	128 - 267	1e-10	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	129 - 274	1.1e-11	Prp19 WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	139 - 339	1.9e-07	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	142 - 274	8.9e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	172 - 336	1.5e-09	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	183 - 316	2e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	189 - 334	1.9e-16	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	190 - 305	9.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	191 - 320	5.1e-06	WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd	PF25047	218 - 309	3e-13	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	220 - 310	5.7e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	221 - 338	1.1e-09	THOC3 beta-propeller domain
WDR55	PF24796	222 - 305	3.5e-06	WDR55
WD40_Prp19	PF24814	223 - 339	1e-08	Prp19 WD40 domain
Beta-prop_DCAF4	PF23761	233 - 321	6.3e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40_CDC20-Fz	PF24807	263 - 344	1.2e-06	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23730
fragaria_vesca	FvH4_7g29370
malus_domestica	MD01G1199700.v1.1 MD07G1267700.v1.1
prunus_persica	Prupe.2G293400_v2.0.a1
pyrus_communis	pycom01g20980 pycom07g24530
rosa_chinensis	RchiOBHm_Chr1g0377161
rosa_laevigata	RLG00000026540
rosa_multiflora	Rmu_ssc0000155.1_g000016
rosa_roxburghii	Rroxscaffold_4G00281420
rosa_rugosa	Rorug01G0401700
rosa_samantha	Rh1AG419100 Rh1BG378200 Rh1CG392200 Rh1DG408900
rosa_wichuraiana	Rw1G036760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	154, 425, 455
Acc36I	ACCTGC	1 cut(s)	1162
AccB1I	GGYRCC	1 cut(s)	176
AclWI	GGATC	5 cut(s)	408, 421, 545, 677, 1162
AcoI	YGGCCR	2 cut(s)	421, 705
AcsI	RAATTY	1 cut(s)	45
AcyI	GRCGYC	2 cut(s)	177, 453
AfaI	GTAC	4 cut(s)	803, 826, 899, 998
AfiI	CCNNNNNNNGG	7 cut(s)	423, 430, 560, 582, 692, 769, 829
AgsI	TTSAA	4 cut(s)	118, 211, 318, 859
AjiI	CACGTC	1 cut(s)	1203
AleI	CACNNNNGTG	1 cut(s)	1142
AluBI	AGCT	5 cut(s)	9, 388, 839, 862, 924
AluI	AGCT	5 cut(s)	9, 388, 839, 862, 924
Alw21I	GWGCWC	2 cut(s)	510, 1139
Alw26I	GTCTC	2 cut(s)	146, 887
Alw44I	GTGCAC	1 cut(s)	1135
AlwI	GGATC	5 cut(s)	408, 421, 545, 677, 1162
AlwNI	CAGNNNCTG	1 cut(s)	525
AoxI	GGCC	6 cut(s)	407, 421, 432, 705, 729, 760
ApaI	GGGCCC	1 cut(s)	764
ApaLI	GTGCAC	1 cut(s)	1135
ApeKI	GCWGC	6 cut(s)	393, 609, 640, 669, 839, 1049
ApoI	RAATTY	1 cut(s)	45
Asp700I	GAANNNNTTC	1 cut(s)	8
AspLEI	GCGC	9 cut(s)	179, 501, 519, 609, 655, 698, 700, 719, 1091
AspS9I	GGNCC	6 cut(s)	408, 601, 721, 760, 761, 1027
AsuC2I	CCSGG	3 cut(s)	411, 425, 764
AsuHPI	GGTGA	3 cut(s)	844, 1088, 1136
AvaII	GGWCC	3 cut(s)	601, 721, 1027
AxyI	CCTNAGG	1 cut(s)	16
BaeGI	GKGCMC	3 cut(s)	764, 909, 1139
BamHI	GGATCC	1 cut(s)	413
BanI	GGYRCC	1 cut(s)	176
BanII	GRGCYC	2 cut(s)	764, 1020
BarI	GAAGNNNNNNTAC	2 cut(s)	66, 98
BauI	CACGAG	1 cut(s)	1031
BbsI	GAAGAC	1 cut(s)	766
Bbv12I	GWGCWC	2 cut(s)	510, 1139
BbvI	GCAGC	6 cut(s)	405, 596, 652, 656, 826, 1036
BccI	CCATC	4 cut(s)	223, 497, 575, 1154
BceAI	ACGGC	5 cut(s)	436, 466, 526, 692, 807
BcnI	CCSGG	3 cut(s)	411, 425, 764
BcoDI	GTCTC	2 cut(s)	146, 887
BfmI	CTRYAG	1 cut(s)	590
BfoI	RGCGCY	1 cut(s)	180
BfuAI	ACCTGC	1 cut(s)	1162
BlpI	GCTNAGC	1 cut(s)	389
Bme1390I	CCNGG	3 cut(s)	411, 425, 764
Bme18I	GGWCC	3 cut(s)	601, 721, 1027
BmgBI	CACGTC	1 cut(s)	1203
BmgT120I	GGNCC	6 cut(s)	408, 601, 721, 760, 761, 1027
BmiI	GGNNCC	7 cut(s)	178, 415, 574, 602, 603, 762, 1028
BmrFI	CCNGG	3 cut(s)	411, 425, 764
BmrI	ACTGGG	1 cut(s)	639
BmsI	GCATC	4 cut(s)	191, 343, 704, 956
BmuI	ACTGGG	1 cut(s)	639
BpiI	GAAGAC	1 cut(s)	766
BpmI	CTGGAG	1 cut(s)	547
Bpu1102I	GCTNAGC	1 cut(s)	389
BpuMI	CCSGG	3 cut(s)	411, 425, 764
BsaAI	YACGTR	2 cut(s)	349, 805
BsaHI	GRCGYC	2 cut(s)	177, 453
BsaJI	CCNNGG	2 cut(s)	172, 193
BsaWI	WCCGGW	3 cut(s)	553, 661, 723
Bsc4I	CCNNNNNNNGG	7 cut(s)	423, 430, 560, 582, 692, 769, 829
Bse118I	RCCGGY	3 cut(s)	236, 984, 1052
Bse1I	ACTGG	1 cut(s)	645
Bse21I	CCTNAGG	1 cut(s)	16
BseDI	CCNNGG	2 cut(s)	172, 193
BseGI	GGATG	3 cut(s)	182, 719, 971
BseLI	CCNNNNNNNGG	7 cut(s)	423, 430, 560, 582, 692, 769, 829
BseMII	CTCAG	2 cut(s)	30, 403
BseNI	ACTGG	1 cut(s)	645
BsePI	GCGCGC	1 cut(s)	696
BseRI	GAGGAG	4 cut(s)	4, 1094, 1142, 1201
BseSI	GKGCMC	3 cut(s)	764, 909, 1139
BseX3I	CGGCCG	1 cut(s)	421
BseXI	GCAGC	6 cut(s)	405, 596, 652, 656, 826, 1036
BsgI	GTGCAG	1 cut(s)	659
Bsh1285I	CGRYCG	1 cut(s)	424
BshFI	GGCC	6 cut(s)	409, 423, 434, 707, 731, 762
BshNI	GGYRCC	1 cut(s)	176
BsiEI	CGRYCG	1 cut(s)	424
BsiHKAI	GWGCWC	2 cut(s)	510, 1139
BsiWI	CGTACG	2 cut(s)	801, 824
BslFI	GGGAC	3 cut(s)	598, 614, 881
BslI	CCNNNNNNNGG	7 cut(s)	423, 430, 560, 582, 692, 769, 829
BsmAI	GTCTC	2 cut(s)	146, 887
BsmBI	CGTCTC	1 cut(s)	887
BsmFI	GGGAC	3 cut(s)	598, 614, 881
BsmI	GAATGC	2 cut(s)	544, 629
BsnI	GGCC	6 cut(s)	409, 423, 434, 707, 731, 762
Bsp120I	GGGCCC	1 cut(s)	760
Bsp1286I	GDGCHC	5 cut(s)	510, 764, 909, 1020, 1139
Bsp143I	GATC	5 cut(s)	107, 413, 550, 682, 1154
Bsp1720I	GCTNAGC	1 cut(s)	389
BspANI	GGCC	6 cut(s)	409, 423, 434, 707, 731, 762
BspCNI	CTCAG	2 cut(s)	29, 402
BspLI	GGNNCC	7 cut(s)	178, 415, 574, 602, 603, 762, 1028
BspMI	ACCTGC	1 cut(s)	1162
BspPI	GGATC	5 cut(s)	408, 421, 545, 677, 1162
BspT107I	GGYRCC	1 cut(s)	176
BsrFI	RCCGGY	3 cut(s)	236, 984, 1052
BsrI	ACTGG	1 cut(s)	645
BssAI	RCCGGY	3 cut(s)	236, 984, 1052
BssECI	CCNNGG	2 cut(s)	172, 193
BssHII	GCGCGC	1 cut(s)	696
BssMI	GATC	5 cut(s)	107, 413, 550, 682, 1154
BssNI	GRCGYC	2 cut(s)	177, 453
BssSI	CACGAG	1 cut(s)	1031
Bst2BI	CACGAG	1 cut(s)	1031
Bst4CI	ACNGT	5 cut(s)	151, 226, 796, 829, 917
BstACI	GRCGYC	2 cut(s)	177, 453
BstBAI	YACGTR	2 cut(s)	349, 805
BstDEI	CTNAG	3 cut(s)	16, 229, 389
BstDSI	CCRYGG	1 cut(s)	172
BstF5I	GGATG	3 cut(s)	182, 719, 971
BstH2I	RGCGCY	1 cut(s)	180
BstHHI	GCGC	9 cut(s)	179, 501, 519, 609, 655, 698, 700, 719, 1091
BstKTI	GATC	5 cut(s)	110, 416, 553, 685, 1157
BstMAI	GTCTC	2 cut(s)	146, 887
BstMBI	GATC	5 cut(s)	107, 413, 550, 682, 1154
BstMCI	CGRYCG	1 cut(s)	424
BstMWI	GCNNNNNNNGC	8 cut(s)	399, 505, 514, 615, 624, 704, 1049, 1138
BstNSI	RCATGY	1 cut(s)	360
BstSCI	CCNGG	3 cut(s)	409, 423, 762
BstSFI	CTRYAG	1 cut(s)	590
BstSLI	GKGCMC	3 cut(s)	764, 909, 1139
BstV1I	GCAGC	6 cut(s)	405, 596, 652, 656, 826, 1036
BstV2I	GAAGAC	1 cut(s)	766
BstX2I	RGATCY	1 cut(s)	413
BstXI	CCANNNNNNTGG	1 cut(s)	1037
BstYI	RGATCY	1 cut(s)	413
BstZI	CGGCCG	1 cut(s)	421
Bsu36I	CCTNAGG	1 cut(s)	16
BsuRI	GGCC	6 cut(s)	409, 423, 434, 707, 731, 762
BtgI	CCRYGG	1 cut(s)	172
BtrI	CACGTC	1 cut(s)	1203
BtsCI	GGATG	3 cut(s)	182, 719, 971
BtsI	GCAGTG	1 cut(s)	1139
BtsIMutI	CAGTG	2 cut(s)	652, 1139
BveI	ACCTGC	1 cut(s)	1162
CaiI	CAGNNNCTG	1 cut(s)	525
CfoI	GCGC	9 cut(s)	179, 501, 519, 609, 655, 698, 700, 719, 1091
Cfr10I	RCCGGY	3 cut(s)	236, 984, 1052
Cfr13I	GGNCC	6 cut(s)	408, 601, 721, 760, 761, 1027
Cfr42I	CCGCGG	1 cut(s)	175
CpoI	CGGWCCG	1 cut(s)	721
CseI	GACGC	3 cut(s)	442, 555, 659
Csp6I	GTAC	4 cut(s)	802, 825, 898, 997
CspI	CGGWCCG	1 cut(s)	721
CviAII	CATG	2 cut(s)	357, 430
CviQI	GTAC	4 cut(s)	802, 825, 898, 997
DdeI	CTNAG	3 cut(s)	16, 229, 389
DinI	GGCGCC	1 cut(s)	178
DpnI	GATC	5 cut(s)	109, 415, 552, 684, 1156
DpnII	GATC	5 cut(s)	107, 413, 550, 682, 1154
DrdI	GACNNNNNNGTC	3 cut(s)	154, 425, 455
DseDI	GACNNNNNNGTC	3 cut(s)	154, 425, 455
EaeI	YGGCCR	2 cut(s)	421, 705
EagI	CGGCCG	1 cut(s)	421
EciI	GGCGGA	2 cut(s)	255, 347
EclXI	CGGCCG	1 cut(s)	421
Eco147I	AGGCCT	1 cut(s)	731
Eco24I	GRGCYC	2 cut(s)	764, 1020
Eco47I	GGWCC	3 cut(s)	601, 721, 1027
Eco52I	CGGCCG	1 cut(s)	421
Eco81I	CCTNAGG	1 cut(s)	16
EcoO109I	RGGNCCY	1 cut(s)	601
EcoT22I	ATGCAT	1 cut(s)	358
EcoT38I	GRGCYC	2 cut(s)	764, 1020
EgeI	GGCGCC	1 cut(s)	178
EheI	GGCGCC	1 cut(s)	178
Esp3I	CGTCTC	1 cut(s)	887
FaeI	CATG	2 cut(s)	360, 433
FaiI	YATR	4 cut(s)	329, 358, 431, 770
FaqI	GGGAC	3 cut(s)	598, 614, 881
FatI	CATG	2 cut(s)	356, 429
FauI	CCCGC	8 cut(s)	388, 506, 611, 612, 751, 876, 962, 1141
FokI	GGATG	3 cut(s)	169, 726, 978
FriOI	GRGCYC	2 cut(s)	764, 1020
GlaI	GCGC	9 cut(s)	178, 500, 518, 608, 654, 697, 699, 718, 1090
GsuI	CTGGAG	1 cut(s)	547
HaeII	RGCGCY	1 cut(s)	180
HaeIII	GGCC	6 cut(s)	409, 423, 434, 707, 731, 762
HgaI	GACGC	3 cut(s)	442, 555, 659
HhaI	GCGC	9 cut(s)	179, 501, 519, 609, 655, 698, 700, 719, 1091
Hin1I	GRCGYC	2 cut(s)	177, 453
Hin1II	CATG	2 cut(s)	360, 433
Hin6I	GCGC	9 cut(s)	177, 499, 517, 607, 653, 696, 698, 717, 1089
HinP1I	GCGC	9 cut(s)	177, 499, 517, 607, 653, 696, 698, 717, 1089
HindIII	AAGCTT	1 cut(s)	7
HinfI	GANTC	4 cut(s)	21, 243, 522, 878
HphI	GGTGA	3 cut(s)	844, 1088, 1136
Hpy166II	GTNNAC	4 cut(s)	265, 997, 1030, 1137
Hpy188III	TCNNGA	5 cut(s)	18, 51, 145, 526, 815
Hpy8I	GTNNAC	4 cut(s)	265, 997, 1030, 1137
Hpy99I	CGWCG	7 cut(s)	422, 452, 458, 506, 581, 782, 1207
HpyAV	CCTTC	3 cut(s)	68, 721, 1079
HpyCH4III	ACNGT	5 cut(s)	151, 226, 796, 829, 917
HpyCH4IV	ACGT	5 cut(s)	348, 800, 804, 896, 1202
HpyCH4V	TGCA	6 cut(s)	356, 375, 640, 842, 1137, 1171
HpyF10VI	GCNNNNNNNGC	8 cut(s)	399, 505, 514, 615, 624, 704, 1049, 1138
HpyF3I	CTNAG	3 cut(s)	16, 229, 389
HpySE526I	ACGT	5 cut(s)	348, 800, 804, 896, 1202
Hsp92I	GRCGYC	2 cut(s)	177, 453
Hsp92II	CATG	2 cut(s)	360, 433
HspAI	GCGC	9 cut(s)	177, 499, 517, 607, 653, 696, 698, 717, 1089
KasI	GGCGCC	1 cut(s)	176
KflI	GGGWCCC	1 cut(s)	601
KspI	CCGCGG	1 cut(s)	175
Kzo9I	GATC	5 cut(s)	107, 413, 550, 682, 1154
LmnI	GCTCC	5 cut(s)	528, 563, 578, 921, 1129
Lsp1109I	GCAGC	6 cut(s)	405, 596, 652, 656, 826, 1036
LweI	GCATC	4 cut(s)	191, 343, 704, 956
MaeII	ACGT	5 cut(s)	348, 800, 804, 896, 1202
MaeIII	GTNAC	5 cut(s)	457, 647, 796, 832, 1194
MalI	GATC	5 cut(s)	109, 415, 552, 684, 1156
MboI	GATC	5 cut(s)	107, 413, 550, 682, 1154
MboII	GAAGA	2 cut(s)	484, 766
MfeI	CAATTG	1 cut(s)	121
MflI	RGATCY	1 cut(s)	413
MhlI	GDGCHC	5 cut(s)	510, 764, 909, 1020, 1139
MluCI	AATT	5 cut(s)	45, 80, 121, 289, 960
Mly113I	GGCGCC	1 cut(s)	177
MlyI	GAGTC	1 cut(s)	531
MmeI	TCCRAC	8 cut(s)	186, 210, 441, 600, 691, 899, 954, 1185
Mph1103I	ATGCAT	1 cut(s)	358
MroXI	GAANNNNTTC	1 cut(s)	8
MslI	CAYNNNNRTG	2 cut(s)	351, 1142
MspA1I	CMGCKG	3 cut(s)	174, 402, 839
MspR9I	CCNGG	3 cut(s)	411, 425, 764
MunI	CAATTG	1 cut(s)	121
Mva1269I	GAATGC	2 cut(s)	544, 629
MwoI	GCNNNNNNNGC	8 cut(s)	399, 505, 514, 615, 624, 704, 1049, 1138
NarI	GGCGCC	1 cut(s)	177
NciI	CCSGG	3 cut(s)	411, 425, 764
NdeII	GATC	5 cut(s)	107, 413, 550, 682, 1154
NlaIII	CATG	2 cut(s)	360, 433
NlaIV	GGNNCC	7 cut(s)	178, 415, 574, 602, 603, 762, 1028
NmuCI	GTSAC	5 cut(s)	457, 647, 796, 832, 1194
NsiI	ATGCAT	1 cut(s)	358
NspI	RCATGY	1 cut(s)	360
OliI	CACNNNNGTG	1 cut(s)	1142
PaeI	GCATGC	1 cut(s)	360
PauI	GCGCGC	1 cut(s)	696
PceI	AGGCCT	1 cut(s)	731
PcsI	WCGNNNNNNNCGW	3 cut(s)	201, 453, 501
PctI	GAATGC	2 cut(s)	544, 629
PdmI	GAANNNNTTC	1 cut(s)	8
PfeI	GAWTC	3 cut(s)	21, 243, 878
Pfl23II	CGTACG	2 cut(s)	801, 824
PflFI	GACNNNGTC	1 cut(s)	452
PleI	GAGTC	1 cut(s)	530
PluTI	GGCGCC	1 cut(s)	180
PpsI	GAGTC	1 cut(s)	530
Ppu21I	YACGTR	2 cut(s)	349, 805
PpuMI	RGGWCCY	1 cut(s)	601
Psp5II	RGGWCCY	1 cut(s)	601
PspLI	CGTACG	2 cut(s)	801, 824
PspN4I	GGNNCC	7 cut(s)	178, 415, 574, 602, 603, 762, 1028
PspOMI	GGGCCC	1 cut(s)	760
PspPI	GGNCC	6 cut(s)	408, 601, 721, 760, 761, 1027
PspPPI	RGGWCCY	1 cut(s)	601
PstNI	CAGNNNCTG	1 cut(s)	525
PsuI	RGATCY	1 cut(s)	413
PsyI	GACNNNGTC	1 cut(s)	452
PteI	GCGCGC	1 cut(s)	696
PvuII	CAGCTG	1 cut(s)	839
RsaI	GTAC	4 cut(s)	803, 826, 899, 998
RsaNI	GTAC	4 cut(s)	802, 825, 898, 997
RseI	CAYNNNNRTG	2 cut(s)	351, 1142
Rsr2I	CGGWCCG	1 cut(s)	721
RsrII	CGGWCCG	1 cut(s)	721
SacII	CCGCGG	1 cut(s)	175
Sau3AI	GATC	5 cut(s)	107, 413, 550, 682, 1154
Sau96I	GGNCC	6 cut(s)	408, 601, 721, 760, 761, 1027
SchI	GAGTC	1 cut(s)	531
ScrFI	CCNGG	3 cut(s)	411, 425, 764
SduI	GDGCHC	5 cut(s)	510, 764, 909, 1020, 1139
SfaNI	GCATC	4 cut(s)	191, 343, 704, 956
SfcI	CTRYAG	1 cut(s)	590
SfoI	GGCGCC	1 cut(s)	178
Sfr303I	CCGCGG	1 cut(s)	175
SgrAI	CRCCGGYG	1 cut(s)	236
SgrBI	CCGCGG	1 cut(s)	175
SinI	GGWCC	3 cut(s)	601, 721, 1027
SmiMI	CAYNNNNRTG	2 cut(s)	351, 1142
SphI	GCATGC	1 cut(s)	360
Sse9I	AATT	5 cut(s)	45, 80, 121, 289, 960
SseBI	AGGCCT	1 cut(s)	731
SspDI	GGCGCC	1 cut(s)	176
StuI	AGGCCT	1 cut(s)	731
StyD4I	CCNGG	3 cut(s)	409, 423, 762
TaaI	ACNGT	5 cut(s)	151, 226, 796, 829, 917
TaiI	ACGT	5 cut(s)	351, 803, 807, 899, 1205
TaqI	TCGA	5 cut(s)	146, 204, 495, 777, 816
TasI	AATT	5 cut(s)	45, 80, 121, 289, 960
TauI	GCSGC	5 cut(s)	174, 177, 182, 409, 696
TfiI	GAWTC	3 cut(s)	21, 243, 878
TscAI	CASTG	2 cut(s)	652, 1139
TseFI	GTSAC	5 cut(s)	457, 647, 796, 832, 1194
TseI	GCWGC	6 cut(s)	393, 609, 640, 669, 839, 1049
Tsp45I	GTSAC	5 cut(s)	457, 647, 796, 832, 1194
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	6 cut(s)	476, 675, 795, 860, 887, 966
TspRI	CASTG	2 cut(s)	652, 1139
Tth111I	GACNNNGTC	1 cut(s)	452
VneI	GTGCAC	1 cut(s)	1135
VpaK11BI	GGWCC	3 cut(s)	601, 721, 1027
XapI	RAATTY	1 cut(s)	45
XceI	RCATGY	1 cut(s)	360
XmnI	GAANNNNTTC	1 cut(s)	8
Zsp2I	ATGCAT	1 cut(s)	358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.