Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0014091

Physical Location & Seq

Genomic Coordinates Reverse (-)

3695022 .. 3696311

Gene Structure 1290 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026540

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGCTAGGGTACGGGAGCCTTTGGTTGGTACAGAAACACCCCCAATGCCTCCAAAACTTAGCATCTCCACCACCACCGCCCCTTAACCATGACTGGGCGATGAACCCACCATTCCATTTCCCAGCCCCACAGCCATTCCAAAACCAGAACCAAACCCAAAACCCAGACCAACAACAACAACAAGTTCAAGATAACAAACCCACCGCCGATGATCATCCTCTTCATAAGTCCAGGTTCGAATGGGACTTCACTCTCTCCACGGTAGTCTCCTCCCCCACCGCCACCGGCGCTGTCTCCAACACTCTTGGAGTAATCGAGTTCGACCCATCGGAAACTGTTCTAGCCACCGGAGGCATAGCCAGGAAGATTAGAATCTACAGCGTCAAGTCTCTCCTGCCGAGTCATGAACAGCCTAGGGACGACGTCGTTTCGCTAGACCACGCTGCTGCATGCGACTATTACATTTGCACCCCCGCCAAGCTCAGCAGCCTCCGCTGGCGGCCCGGGTCGAGCGGCCGGGTCCTCGGTTCGGGGGACTACGACGGCGTCGTCACCGAGTACGATCTCGAGTCCCACCTCCCCATCTTCGAGCGCGACGAGCACGGCGGGCGCCGGGTCTGGAGCCTCGACTACTCGGATTCGGATCCGGTTCTCGGGGCATCCGGATCCGACGACGGGACCCTTCAAATGTGGGACCCTCGATGCGAGGGCGGGGACTGTGTGGCTAGAGTGGCTCCTTCCGAAGCGGGCAACCCGGTCTGTTGCGTGGAGTTCAATCCCTTCGGTGGGCAGCTCCTGGCCGTCGGATGCGCTGACCGGAAAGCCTACGTGTACGATGTGAGGAGAATGGCGGGCCCGGTTTTGAGTTTTGACGGCCACGATAAGACCGTGACGTACGTCAGGTTCCTTAATGGCCGTTCGATGGTGTCAGCTGGAACGGACGGGTGTCTGAAGATGTGGAGCGTGGATGACGCGCGGGAAATTCGGACGTATTCGGGTCACACCAACAATCGGAGCTTTGTCGGGTTATCAGTTTGGAGAAACGGTGGGCTTCTCGGGTGCGGGTCAGAAAATAATCAGGTATTTGTGTATGATGTTAGGTGGGGTGAGCCTGTTTGGGTGCATGGGTTTGGACCAATGGGAAGCGAGGGTTGTGATGACCGTGGGTTTGTGAGCAGTGTGTGCTGGCGGCAGATGGGCGGTGAGGAACAGTGTACCCTCGTGGCGGGTGGATCCGATGGGGTTTTGCAGGTTTTTGTGGGTAGAAGAAAGTCATTAGCTTCGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009657 GO:0009658 GO:0009791 GO:0009812 GO:0009987 GO:0010114 GO:0010119 GO:0010218 GO:0010224 GO:0010228 GO:0016043 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043393 GO:0043412 GO:0043496 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051098 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40

Protein Analysis

430

Amino Acids

46.98

Weight (kDa)

5.22

Isoelectric Point (pI)

47.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	101 - 367	2.5e-22	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	159 - 298	1e-10	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	160 - 367	4e-18	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	171 - 339	4.3e-13	TEP-1 second beta-propeller
WDR55	PF24796	174 - 366	7.2e-08	WDR55
Beta-prop_EML_2	PF23414	177 - 303	2.2e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	203 - 365	2.9e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	214 - 368	5.6e-06	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	220 - 362	1.6e-13	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	251 - 340	7.1e-08	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	252 - 370	7.7e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	252 - 366	2.3e-13	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	252 - 364	8e-06	WDR3 second beta-propeller domain
Beta-prop_DCAF4	PF23761	267 - 352	8.8e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40_CDC20-Fz	PF24807	292 - 419	1.4e-07	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23730
fragaria_vesca	FvH4_7g29370
malus_domestica	MD01G1199700.v1.1 MD07G1267700.v1.1
prunus_persica	Prupe.2G293400_v2.0.a1
pyrus_communis	pycom01g20980 pycom07g24530
rosa_chinensis	RchiOBHm_Chr1g0377161
rosa_laevigata	RLG00000026540
rosa_multiflora	Rmu_ssc0000155.1_g000016
rosa_roxburghii	Rroxscaffold_4G00281420
rosa_rugosa	Rorug01G0401700
rosa_samantha	Rh1AG419100 Rh1BG378200 Rh1CG392200 Rh1DG408900
rosa_wichuraiana	Rw1G036760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	548
AatII	GACGTC	1 cut(s)	426
Acc36I	ACCTGC	1 cut(s)	1240
AccB1I	GGYRCC	1 cut(s)	609
AccBSI	CCGCTC	1 cut(s)	513
AccII	CGCG	3 cut(s)	594, 974, 976
AccIII	TCCGGA	1 cut(s)	662
AclWI	GGATC	6 cut(s)	638, 651, 660, 673, 1227, 1240
AcoI	YGGCCR	4 cut(s)	514, 798, 874, 913
AcsI	RAATTY	1 cut(s)	981
AcuI	CTGAAG	1 cut(s)	971
AcyI	GRCGYC	3 cut(s)	423, 546, 610
AfaI	GTAC	6 cut(s)	11, 30, 560, 833, 896, 1216
AflIII	ACRYGT	1 cut(s)	828
AgsI	TTSAA	3 cut(s)	188, 686, 775
AjnI	CCWGG	3 cut(s)	230, 359, 795
AluBI	AGCT	5 cut(s)	481, 793, 932, 1017, 1280
AluI	AGCT	5 cut(s)	481, 793, 932, 1017, 1280
Alw21I	GWGCWC	1 cut(s)	603
Alw26I	GTCTC	3 cut(s)	271, 298, 393
AlwI	GGATC	6 cut(s)	638, 651, 660, 673, 1227, 1240
AlwNI	CAGNNNCTG	1 cut(s)	796
Ama87I	CYCGRG	4 cut(s)	503, 566, 653, 1055
Aor13HI	TCCGGA	1 cut(s)	662
AoxI	GGCC	6 cut(s)	500, 514, 798, 853, 874, 913
ApaI	GGGCCC	1 cut(s)	857
ApeKI	GCWGC	4 cut(s)	443, 446, 486, 790
ApoI	RAATTY	1 cut(s)	981
ArsI	GACNNNNNNTTYG	2 cut(s)	1151, 1183
Asp700I	GAANNNNTTC	1 cut(s)	336
AspA2I	CCTAGG	1 cut(s)	413
AspLEI	GCGC	5 cut(s)	290, 594, 612, 812, 976
AspS9I	GGNCC	7 cut(s)	501, 520, 678, 694, 853, 854, 1133
AsuC2I	CCSGG	6 cut(s)	504, 505, 518, 614, 755, 857
AsuHPI	GGTGA	3 cut(s)	544, 1118, 1214
AsuII	TTCGAA	1 cut(s)	237
AvaI	CYCGRG	4 cut(s)	503, 566, 653, 1055
AvaII	GGWCC	4 cut(s)	520, 678, 694, 1133
AvrII	CCTAGG	1 cut(s)	413
BaeGI	GKGCMC	1 cut(s)	857
BamHI	GGATCC	3 cut(s)	643, 665, 1232
BanI	GGYRCC	1 cut(s)	609
BanII	GRGCYC	1 cut(s)	857
BauI	CACGAG	1 cut(s)	1220
Bbv12I	GWGCWC	1 cut(s)	603
BbvI	GCAGC	4 cut(s)	430, 433, 498, 802
BccI	CCATC	5 cut(s)	334, 590, 916, 1189, 1232
BceAI	ACGGC	5 cut(s)	559, 619, 785, 889, 900
BciT130I	CCWGG	3 cut(s)	232, 361, 797
BclI	TGATCA	1 cut(s)	211
BcnI	CCSGG	6 cut(s)	504, 505, 518, 614, 755, 857
BcoDI	GTCTC	3 cut(s)	271, 298, 393
BfaI	CTAG	5 cut(s)	5, 341, 414, 434, 726
BfmI	CTRYAG	1 cut(s)	376
BfoI	RGCGCY	2 cut(s)	291, 613
BfuAI	ACCTGC	1 cut(s)	1240
BisI	GCNGC	7 cut(s)	444, 447, 487, 500, 514, 791, 1190
BlnI	CCTAGG	1 cut(s)	413
BlpI	GCTNAGC	1 cut(s)	482
BlsI	GCNGC	7 cut(s)	445, 448, 488, 501, 515, 792, 1191
Bme1390I	CCNGG	9 cut(s)	232, 361, 504, 505, 518, 614, 755, 797, 857
Bme18I	GGWCC	4 cut(s)	520, 678, 694, 1133
BmeT110I	CYCGRG	4 cut(s)	503, 566, 653, 1055
BmgT120I	GGNCC	7 cut(s)	501, 520, 678, 694, 853, 854, 1133
BmrFI	CCNGG	9 cut(s)	232, 361, 504, 505, 518, 614, 755, 797, 857
BmrI	ACTGGG	1 cut(s)	103
BmsI	GCATC	4 cut(s)	71, 668, 692, 797
BmuI	ACTGGG	1 cut(s)	103
BoxI	GACNNNNGTC	2 cut(s)	896, 945
BpmI	CTGGAG	1 cut(s)	640
Bpu1102I	GCTNAGC	1 cut(s)	482
Bpu14I	TTCGAA	1 cut(s)	237
BpuMI	CCSGG	6 cut(s)	504, 505, 518, 614, 755, 857
BsaAI	YACGTR	1 cut(s)	829
BsaBI	GATNNNNATC	3 cut(s)	213, 371, 642
BsaHI	GRCGYC	3 cut(s)	423, 546, 610
BsaJI	CCNNGG	5 cut(s)	258, 413, 503, 523, 1162
BsaWI	WCCGGW	4 cut(s)	347, 646, 662, 816
BsaXI	ACNNNNNCTCC	2 cut(s)	1030, 1060
Bse118I	RCCGGY	1 cut(s)	284
Bse1I	ACTGG	1 cut(s)	98
Bse8I	GATNNNNATC	3 cut(s)	213, 371, 642
BseAI	TCCGGA	1 cut(s)	662
BseBI	CCWGG	3 cut(s)	232, 361, 797
BseDI	CCNNGG	5 cut(s)	258, 413, 503, 523, 1162
BseGI	GGATG	4 cut(s)	214, 659, 812, 973
BseJI	GATNNNNATC	3 cut(s)	213, 371, 642
BseMII	CTCAG	1 cut(s)	496
BseNI	ACTGG	1 cut(s)	98
BseRI	GAGGAG	2 cut(s)	259, 856
BseSI	GKGCMC	1 cut(s)	857
BseX3I	CGGCCG	1 cut(s)	514
BseXI	GCAGC	4 cut(s)	430, 433, 498, 802
BseYI	CCCAGC	1 cut(s)	121
Bsh1236I	CGCG	3 cut(s)	594, 974, 976
Bsh1285I	CGRYCG	1 cut(s)	517
BshFI	GGCC	6 cut(s)	502, 516, 800, 855, 876, 915
BshNI	GGYRCC	1 cut(s)	609
BsiEI	CGRYCG	1 cut(s)	517
BsiHKAI	GWGCWC	1 cut(s)	603
BsiHKCI	CYCGRG	4 cut(s)	503, 566, 653, 1055
BsiWI	CGTACG	1 cut(s)	894
BslFI	GGGAC	7 cut(s)	257, 431, 548, 556, 691, 707, 728
BsmAI	GTCTC	3 cut(s)	271, 298, 393
BsmFI	GGGAC	7 cut(s)	257, 431, 548, 556, 691, 707, 728
BsnI	GGCC	6 cut(s)	502, 516, 800, 855, 876, 915
BsoBI	CYCGRG	4 cut(s)	503, 566, 653, 1055
Bsp119I	TTCGAA	1 cut(s)	237
Bsp120I	GGGCCC	1 cut(s)	853
Bsp1286I	GDGCHC	2 cut(s)	603, 857
Bsp13I	TCCGGA	1 cut(s)	662
Bsp143I	GATC	5 cut(s)	211, 562, 643, 665, 1232
Bsp1720I	GCTNAGC	1 cut(s)	482
BspANI	GGCC	6 cut(s)	502, 516, 800, 855, 876, 915
BspCNI	CTCAG	1 cut(s)	495
BspEI	TCCGGA	1 cut(s)	662
BspFNI	CGCG	3 cut(s)	594, 974, 976
BspHI	TCATGA	1 cut(s)	403
BspMI	ACCTGC	1 cut(s)	1240
BspPI	GGATC	6 cut(s)	638, 651, 660, 673, 1227, 1240
BspT104I	TTCGAA	1 cut(s)	237
BspT107I	GGYRCC	1 cut(s)	609
BsrBI	CCGCTC	1 cut(s)	513
BsrFI	RCCGGY	1 cut(s)	284
BsrI	ACTGG	1 cut(s)	98
BssAI	RCCGGY	1 cut(s)	284
BssECI	CCNNGG	5 cut(s)	258, 413, 503, 523, 1162
BssMI	GATC	5 cut(s)	211, 562, 643, 665, 1232
BssNI	GRCGYC	3 cut(s)	423, 546, 610
BssSI	CACGAG	1 cut(s)	1220
BssT1I	CCWWGG	1 cut(s)	413
Bst2BI	CACGAG	1 cut(s)	1220
Bst2UI	CCWGG	3 cut(s)	232, 361, 797
Bst4CI	ACNGT	7 cut(s)	262, 337, 719, 889, 1046, 1163, 1212
Bst6I	CTCTTC	1 cut(s)	225
BstACI	GRCGYC	3 cut(s)	423, 546, 610
BstAPI	GCANNNNNTGC	1 cut(s)	1182
BstBAI	YACGTR	1 cut(s)	829
BstBI	TTCGAA	1 cut(s)	237
BstC8I	GCNNGC	6 cut(s)	451, 497, 608, 748, 853, 1187
BstDEI	CTNAG	2 cut(s)	58, 482
BstDSI	CCRYGG	2 cut(s)	258, 1162
BstF5I	GGATG	4 cut(s)	214, 659, 812, 973
BstFNI	CGCG	3 cut(s)	594, 974, 976
BstH2I	RGCGCY	2 cut(s)	291, 613
BstHHI	GCGC	5 cut(s)	290, 594, 612, 812, 976
BstKTI	GATC	5 cut(s)	214, 565, 646, 668, 1235
BstMAI	GTCTC	3 cut(s)	271, 298, 393
BstMBI	GATC	5 cut(s)	211, 562, 643, 665, 1232
BstMCI	CGRYCG	1 cut(s)	517
BstMWI	GCNNNNNNNGC	6 cut(s)	287, 492, 598, 607, 731, 1182
BstNI	CCWGG	3 cut(s)	232, 361, 797
BstNSI	RCATGY	1 cut(s)	453
BstPAI	GACNNNNGTC	2 cut(s)	896, 945
BstSCI	CCNGG	9 cut(s)	230, 359, 502, 503, 516, 612, 753, 795, 855
BstSFI	CTRYAG	1 cut(s)	376
BstSLI	GKGCMC	1 cut(s)	857
BstUI	CGCG	3 cut(s)	594, 974, 976
BstV1I	GCAGC	4 cut(s)	430, 433, 498, 802
BstX2I	RGATCY	3 cut(s)	643, 665, 1232
BstYI	RGATCY	3 cut(s)	643, 665, 1232
BstZI	CGGCCG	1 cut(s)	514
BsuRI	GGCC	6 cut(s)	502, 516, 800, 855, 876, 915
BtgI	CCRYGG	2 cut(s)	258, 1162
BtgZI	GCGATG	1 cut(s)	113
BtsCI	GGATG	4 cut(s)	214, 659, 812, 973
BtsI	GCAGTG	1 cut(s)	1183
BtsIMutI	CAGTG	2 cut(s)	1183, 1217
BveI	ACCTGC	1 cut(s)	1240
Cac8I	GCNNGC	6 cut(s)	451, 497, 608, 748, 853, 1187
CaiI	CAGNNNCTG	1 cut(s)	796
CciI	TCATGA	1 cut(s)	403
CfoI	GCGC	5 cut(s)	290, 594, 612, 812, 976
Cfr10I	RCCGGY	1 cut(s)	284
Cfr13I	GGNCC	7 cut(s)	501, 520, 678, 694, 853, 854, 1133
Cfr9I	CCCGGG	1 cut(s)	503
CseI	GACGC	3 cut(s)	370, 535, 980
Csp6I	GTAC	6 cut(s)	10, 29, 559, 832, 895, 1215
CviAII	CATG	4 cut(s)	89, 404, 450, 1124
CviQI	GTAC	6 cut(s)	10, 29, 559, 832, 895, 1215
DdeI	CTNAG	2 cut(s)	58, 482
DinI	GGCGCC	1 cut(s)	611
DpnI	GATC	5 cut(s)	213, 564, 645, 667, 1234
DpnII	GATC	5 cut(s)	211, 562, 643, 665, 1232
DrdI	GACNNNNNNGTC	1 cut(s)	548
DseDI	GACNNNNNNGTC	1 cut(s)	548
EaeI	YGGCCR	4 cut(s)	514, 798, 874, 913
EagI	CGGCCG	1 cut(s)	514
Eam1104I	CTCTTC	1 cut(s)	225
EarI	CTCTTC	1 cut(s)	225
EclXI	CGGCCG	1 cut(s)	514
Eco130I	CCWWGG	1 cut(s)	413
Eco24I	GRGCYC	1 cut(s)	857
Eco47I	GGWCC	4 cut(s)	520, 678, 694, 1133
Eco52I	CGGCCG	1 cut(s)	514
Eco57I	CTGAAG	1 cut(s)	971
Eco88I	CYCGRG	4 cut(s)	503, 566, 653, 1055
EcoO109I	RGGNCCY	3 cut(s)	520, 678, 694
EcoRII	CCWGG	3 cut(s)	230, 359, 795
EcoT14I	CCWWGG	1 cut(s)	413
EcoT38I	GRGCYC	1 cut(s)	857
EgeI	GGCGCC	1 cut(s)	611
EheI	GGCGCC	1 cut(s)	611
ErhI	CCWWGG	1 cut(s)	413
FaeI	CATG	4 cut(s)	92, 407, 453, 1127
FaiI	YATR	7 cut(s)	90, 225, 356, 405, 451, 1092, 1125
FaqI	GGGAC	7 cut(s)	257, 431, 548, 556, 691, 707, 728
FatI	CATG	4 cut(s)	88, 403, 449, 1123
FauI	CCCGC	8 cut(s)	481, 599, 704, 739, 844, 969, 1055, 1219
FbaI	TGATCA	1 cut(s)	211
Fnu4HI	GCNGC	7 cut(s)	444, 447, 487, 500, 514, 791, 1190
FokI	GGATG	4 cut(s)	201, 646, 819, 980
FriOI	GRGCYC	1 cut(s)	857
Fsp4HI	GCNGC	7 cut(s)	444, 447, 487, 500, 514, 791, 1190
FspBI	CTAG	5 cut(s)	5, 341, 414, 434, 726
GlaI	GCGC	5 cut(s)	289, 593, 611, 811, 975
GluI	GCNGC	7 cut(s)	444, 447, 487, 500, 514, 791, 1190
GsaI	CCCAGC	1 cut(s)	125
GsuI	CTGGAG	1 cut(s)	640
HaeII	RGCGCY	2 cut(s)	291, 613
HaeIII	GGCC	6 cut(s)	502, 516, 800, 855, 876, 915
HgaI	GACGC	3 cut(s)	370, 535, 980
HhaI	GCGC	5 cut(s)	290, 594, 612, 812, 976
Hin1I	GRCGYC	3 cut(s)	423, 546, 610
Hin1II	CATG	4 cut(s)	92, 407, 453, 1127
Hin6I	GCGC	5 cut(s)	288, 592, 610, 810, 974
HinP1I	GCGC	5 cut(s)	288, 592, 610, 810, 974
HinfI	GANTC	4 cut(s)	372, 400, 569, 638
HphI	GGTGA	3 cut(s)	544, 1118, 1214
Hpy166II	GTNNAC	2 cut(s)	832, 1215
Hpy188III	TCNNGA	5 cut(s)	188, 404, 566, 619, 663
Hpy8I	GTNNAC	2 cut(s)	832, 1215
Hpy99I	CGWCG	8 cut(s)	425, 428, 545, 551, 599, 674, 677, 806
HpyAV	CCTTC	3 cut(s)	692, 747, 790
HpyCH4III	ACNGT	7 cut(s)	262, 337, 719, 889, 1046, 1163, 1212
HpyCH4IV	ACGT	5 cut(s)	423, 828, 893, 897, 989
HpyCH4V	TGCA	4 cut(s)	449, 468, 1123, 1249
HpyF10VI	GCNNNNNNNGC	6 cut(s)	287, 492, 598, 607, 731, 1182
HpyF3I	CTNAG	2 cut(s)	58, 482
HpySE526I	ACGT	5 cut(s)	423, 828, 893, 897, 989
Hsp92I	GRCGYC	3 cut(s)	423, 546, 610
Hsp92II	CATG	4 cut(s)	92, 407, 453, 1127
HspAI	GCGC	5 cut(s)	288, 592, 610, 810, 974
KasI	GGCGCC	1 cut(s)	609
KflI	GGGWCCC	2 cut(s)	678, 694
Kpn2I	TCCGGA	1 cut(s)	662
Ksp22I	TGATCA	1 cut(s)	211
Kzo9I	GATC	5 cut(s)	211, 562, 643, 665, 1232
LmnI	GCTCC	6 cut(s)	15, 621, 739, 798, 960, 1014
Lsp1109I	GCAGC	4 cut(s)	430, 433, 498, 802
LweI	GCATC	4 cut(s)	71, 668, 692, 797
MaeI	CTAG	5 cut(s)	5, 341, 414, 434, 726
MaeII	ACGT	5 cut(s)	423, 828, 893, 897, 989
MaeIII	GTNAC	3 cut(s)	550, 889, 998
MalI	GATC	5 cut(s)	213, 564, 645, 667, 1234
MbiI	CCGCTC	1 cut(s)	513
MboI	GATC	5 cut(s)	211, 562, 643, 665, 1232
MboII	GAAGA	5 cut(s)	212, 376, 577, 964, 1278
MflI	RGATCY	3 cut(s)	643, 665, 1232
MhlI	GDGCHC	2 cut(s)	603, 857
MluCI	AATT	1 cut(s)	981
Mly113I	GGCGCC	1 cut(s)	610
MlyI	GAGTC	2 cut(s)	409, 578
MmeI	TCCRAC	3 cut(s)	321, 693, 784
MroI	TCCGGA	1 cut(s)	662
MroXI	GAANNNNTTC	1 cut(s)	336
MseI	TTAA	2 cut(s)	84, 909
MspA1I	CMGCKG	2 cut(s)	495, 932
MspR9I	CCNGG	9 cut(s)	232, 361, 504, 505, 518, 614, 755, 797, 857
MvaI	CCWGG	3 cut(s)	232, 361, 797
MvnI	CGCG	3 cut(s)	594, 974, 976
MwoI	GCNNNNNNNGC	6 cut(s)	287, 492, 598, 607, 731, 1182
NarI	GGCGCC	1 cut(s)	610
NciI	CCSGG	6 cut(s)	504, 505, 518, 614, 755, 857
NdeII	GATC	5 cut(s)	211, 562, 643, 665, 1232
NlaIII	CATG	4 cut(s)	92, 407, 453, 1127
NmeAIII	GCCGAG	1 cut(s)	423
NmuCI	GTSAC	3 cut(s)	550, 889, 998
NspI	RCATGY	1 cut(s)	453
NspV	TTCGAA	1 cut(s)	237
PaeI	GCATGC	1 cut(s)	453
PaeR7I	CTCGAG	1 cut(s)	566
PagI	TCATGA	1 cut(s)	403
PcsI	WCGNNNNNNNCGW	3 cut(s)	546, 594, 885
PdmI	GAANNNNTTC	1 cut(s)	336
PfeI	GAWTC	2 cut(s)	372, 638
Pfl23II	CGTACG	1 cut(s)	894
PflFI	GACNNNGTC	2 cut(s)	422, 545
PkrI	GCNGC	7 cut(s)	445, 448, 488, 501, 515, 792, 1191
PleI	GAGTC	2 cut(s)	408, 577
PluTI	GGCGCC	1 cut(s)	613
PpsI	GAGTC	2 cut(s)	408, 577
Ppu21I	YACGTR	1 cut(s)	829
PpuMI	RGGWCCY	3 cut(s)	520, 678, 694
PshAI	GACNNNNGTC	2 cut(s)	896, 945
Psp5II	RGGWCCY	3 cut(s)	520, 678, 694
Psp6I	CCWGG	3 cut(s)	230, 359, 795
PspFI	CCCAGC	1 cut(s)	121
PspGI	CCWGG	3 cut(s)	230, 359, 795
PspLI	CGTACG	1 cut(s)	894
PspOMI	GGGCCC	1 cut(s)	853
PspPI	GGNCC	7 cut(s)	501, 520, 678, 694, 853, 854, 1133
PspPPI	RGGWCCY	3 cut(s)	520, 678, 694
PsrI	GAACNNNNNNTAC	2 cut(s)	887, 919
PstNI	CAGNNNCTG	1 cut(s)	796
PsuI	RGATCY	3 cut(s)	643, 665, 1232
PsyI	GACNNNGTC	2 cut(s)	422, 545
PvuII	CAGCTG	1 cut(s)	932
RsaI	GTAC	6 cut(s)	11, 30, 560, 833, 896, 1216
RsaNI	GTAC	6 cut(s)	10, 29, 559, 832, 895, 1215
SaqAI	TTAA	2 cut(s)	84, 909
SatI	GCNGC	7 cut(s)	444, 447, 487, 500, 514, 791, 1190
Sau3AI	GATC	5 cut(s)	211, 562, 643, 665, 1232
Sau96I	GGNCC	7 cut(s)	501, 520, 678, 694, 853, 854, 1133
SchI	GAGTC	2 cut(s)	409, 578
ScrFI	CCNGG	9 cut(s)	232, 361, 504, 505, 518, 614, 755, 797, 857
SduI	GDGCHC	2 cut(s)	603, 857
SfaNI	GCATC	4 cut(s)	71, 668, 692, 797
SfcI	CTRYAG	1 cut(s)	376
SfoI	GGCGCC	1 cut(s)	611
Sfr274I	CTCGAG	1 cut(s)	566
SfuI	TTCGAA	1 cut(s)	237
SgrAI	CRCCGGYG	1 cut(s)	284
SinI	GGWCC	4 cut(s)	520, 678, 694, 1133
SlaI	CTCGAG	1 cut(s)	566
SmaI	CCCGGG	1 cut(s)	505
SmlI	CTYRAG	1 cut(s)	566
SmoI	CTYRAG	1 cut(s)	566
SphI	GCATGC	1 cut(s)	453
Sse9I	AATT	1 cut(s)	981
SspDI	GGCGCC	1 cut(s)	609
SspMI	CTAG	5 cut(s)	5, 341, 414, 434, 726
StyD4I	CCNGG	9 cut(s)	230, 359, 502, 503, 516, 612, 753, 795, 855
StyI	CCWWGG	1 cut(s)	413
TaaI	ACNGT	7 cut(s)	262, 337, 719, 889, 1046, 1163, 1212
TaiI	ACGT	5 cut(s)	426, 831, 896, 900, 992
TaqI	TCGA	9 cut(s)	237, 315, 321, 509, 567, 588, 627, 700, 920
TasI	AATT	1 cut(s)	981
TauI	GCSGC	3 cut(s)	502, 516, 1192
TfiI	GAWTC	2 cut(s)	372, 638
Tru1I	TTAA	2 cut(s)	84, 909
Tru9I	TTAA	2 cut(s)	84, 909
TscAI	CASTG	2 cut(s)	1183, 1217
TseFI	GTSAC	3 cut(s)	550, 889, 998
TseI	GCWGC	4 cut(s)	443, 446, 486, 790
Tsp45I	GTSAC	3 cut(s)	550, 889, 998
TspDTI	ATGAA	3 cut(s)	116, 212, 420
TspGWI	ACGGA	1 cut(s)	953
TspMI	CCCGGG	1 cut(s)	503
TspRI	CASTG	2 cut(s)	1183, 1217
Tth111I	GACNNNGTC	2 cut(s)	422, 545
VpaK11BI	GGWCC	4 cut(s)	520, 678, 694, 1133
XapI	RAATTY	1 cut(s)	981
XceI	RCATGY	1 cut(s)	453
XhoI	CTCGAG	1 cut(s)	566
XmaI	CCCGGG	1 cut(s)	503
XmaJI	CCTAGG	1 cut(s)	413
XmnI	GAANNNNTTC	1 cut(s)	336
XspI	CTAG	5 cut(s)	5, 341, 414, 434, 726
ZraI	GACGTC	1 cut(s)	424

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.