Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0014091

Physical Location & Seq

Genomic Coordinates Reverse (-)

3733845 .. 3737322

Gene Structure 3478 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00281420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGATTGAAACTGATATCCTCATTCAAGTAGAAAATGACTTGTTCATTGATCCTTCCGCATACGCAATCCCACAACCATTCCAAAACCAGAACCAAACCCAAAACCCAGACCAACAACAACAACAAGTTCAAGATAACAAACCCACCGCCGATGATCATCATCTTCATAAGTCCAGGTTCGAATGGGACTTCACTCTCTCCACGGTCGTCTCCTCCCCCACCGCCACCGGCGCCGTCTCCAACACTCTCGGAGTAATCGAGTTCGACCCATCGGAAACTGTTCTAGCCACCGGAGGCATAGCCAGGAAGATTAGAATCTACAGCGTCAAGTCTCTCCTGCCCAGTCATGAACAGCCTAAGGACGACGTCGTTTCGCTAGACCACGCTGCTGCATGCGACTATTACATTTGCACCCCGGCCAAGCTCAGCAGCCTCCGCTGGCGGCCTGGGTCAAGCGGCCGGGTCCTCGGGTCGGGGGACTACGACGGCGTCGTCACGGAGTACGATCTCGAGTCCCACCTCCCCATCTTCGAGCGCGACGAGCACGGCGGGCGCCGGGTCTGGAGCCTCGACTACTCGGATTCGGATCCGGTTCTCGGGGCGTCCGGATCCGACGACGGGACCCTTCAAATGTGGGACCCGCGGTGCGAGGGTGGGGACTGTGTGGCTAGAGTGGCTCCTTCCGAATCGGGTAACCCGGTTTGTTGCGTGGAGTTTAATCCCTTCGGTGGGCCGCTCCTGGCCGTCGGATGCGCAGACCGGAAAGCCTACGTCTATGATGTGAGGAGAATGGCGGGCCCGGTTTTGAGTTTTGACGGCCACGATAAGACCGTGACGTACGTCAGGTTCCTTAACGGCCGTTCGATGGTGTCAGCCGGAACAGACGGGTGTCTGAAGATGTGGAGCGTGGATGACGCGCGGGAAATTCGGACGTATTCGGGTCACACCAACAATCGGAGCTTTGTCGGGTTGTCGGTTTGGAGAAACGGTGGGCTTCTCGGGTGCGGCTCAGAAAATAATCAGGTGTTTGTGTATGATGTTAGGTGGGGTGAGCCTGTTTGGGTGCATGGGTTCGGACCAATGGGAAGCGAGGGTTGTGATGACCGTGGGTTTGTGAGCAGTGTGTGCTGGAGGCAGATGGGCGGTGAGGAACAGTGTACCCTGCTGGCGGGTGGATCCGATGGGGTTTTGCAGGTTTTTGTGGGTAGAAGAAAGTCATTAGCTTCGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009657 GO:0009658 GO:0009791 GO:0009812 GO:0009987 GO:0010114 GO:0010119 GO:0010218 GO:0010224 GO:0010228 GO:0016043 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043393 GO:0043412 GO:0043496 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051098 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40

Protein Analysis

410

Amino Acids

44.87

Weight (kDa)

4.85

Isoelectric Point (pI)

46.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	82 - 348	3.7e-22	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	140 - 279	2.6e-10	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	141 - 348	1.1e-17	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	152 - 320	1e-12	TEP-1 second beta-propeller
Beta-prop_EML_2	PF23414	158 - 285	3.6e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	184 - 346	8.4e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	195 - 349	3.9e-06	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	202 - 316	1e-11	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	232 - 321	1.7e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	233 - 351	2.7e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	233 - 347	7.2e-13	WDR3 first beta-propeller domain
Beta-prop_DCAF4	PF23761	244 - 333	1.5e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40_CDC20-Fz	PF24807	275 - 399	5e-07	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23730
fragaria_vesca	FvH4_7g29370
malus_domestica	MD01G1199700.v1.1 MD07G1267700.v1.1
prunus_persica	Prupe.2G293400_v2.0.a1
pyrus_communis	pycom01g20980 pycom07g24530
rosa_chinensis	RchiOBHm_Chr1g0377161
rosa_laevigata	RLG00000026540
rosa_multiflora	Rmu_ssc0000155.1_g000016
rosa_roxburghii	Rroxscaffold_4G00281420
rosa_rugosa	Rorug01G0401700
rosa_samantha	Rh1AG419100 Rh1BG378200 Rh1CG392200 Rh1DG408900
rosa_wichuraiana	Rw1G036760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	491
AatII	GACGTC	1 cut(s)	369
Acc16I	TGCGCA	1 cut(s)	754
Acc36I	ACCTGC	1 cut(s)	1183
AccB1I	GGYRCC	2 cut(s)	230, 552
AccBSI	CCGCTC	1 cut(s)	736
AccII	CGCG	4 cut(s)	537, 643, 917, 919
AccIII	TCCGGA	1 cut(s)	605
AclWI	GGATC	7 cut(s)	44, 581, 594, 603, 616, 1170, 1183
AcoI	YGGCCR	5 cut(s)	417, 457, 741, 817, 856
AcsI	RAATTY	1 cut(s)	924
AcuI	CTGAAG	1 cut(s)	914
AcyI	GRCGYC	5 cut(s)	231, 366, 489, 553, 602
AfaI	GTAC	3 cut(s)	503, 839, 1159
AfiI	CCNNNNNNNGG	8 cut(s)	472, 596, 618, 728, 729, 865, 954, 1168
AgsI	TTSAA	4 cut(s)	8, 26, 131, 629
AjnI	CCWGG	4 cut(s)	173, 302, 445, 738
AluBI	AGCT	3 cut(s)	424, 960, 1223
AluI	AGCT	3 cut(s)	424, 960, 1223
Alw21I	GWGCWC	1 cut(s)	546
Alw26I	GTCTC	3 cut(s)	214, 241, 336
AlwI	GGATC	7 cut(s)	44, 581, 594, 603, 616, 1170, 1183
Ama87I	CYCGRG	4 cut(s)	467, 509, 596, 998
Aor13HI	TCCGGA	1 cut(s)	605
AoxI	GGCC	8 cut(s)	417, 443, 457, 731, 741, 796, 817, 856
ApaI	GGGCCC	1 cut(s)	800
ApeKI	GCWGC	3 cut(s)	386, 389, 429
ApoI	RAATTY	1 cut(s)	924
ArsI	GACNNNNNNTTYG	2 cut(s)	1094, 1126
Asp700I	GAANNNNTTC	1 cut(s)	279
AspLEI	GCGC	5 cut(s)	233, 537, 555, 755, 919
AspS9I	GGNCC	7 cut(s)	463, 621, 637, 731, 796, 797, 1076
AsuC2I	CCSGG	5 cut(s)	416, 461, 557, 698, 800
AsuHPI	GGTGA	2 cut(s)	1061, 1157
AsuII	TTCGAA	1 cut(s)	180
AvaI	CYCGRG	4 cut(s)	467, 509, 596, 998
AvaII	GGWCC	4 cut(s)	463, 621, 637, 1076
AxyI	CCTNAGG	1 cut(s)	357
BaeGI	GKGCMC	1 cut(s)	800
BamHI	GGATCC	3 cut(s)	586, 608, 1175
BanI	GGYRCC	2 cut(s)	230, 552
BanII	GRGCYC	1 cut(s)	800
Bbv12I	GWGCWC	1 cut(s)	546
BbvI	GCAGC	3 cut(s)	373, 376, 441
BccI	CCATC	5 cut(s)	277, 533, 859, 1132, 1175
BceAI	ACGGC	7 cut(s)	218, 502, 562, 728, 832, 843, 871
BciT130I	CCWGG	4 cut(s)	175, 304, 447, 740
BclI	TGATCA	1 cut(s)	154
BcnI	CCSGG	5 cut(s)	416, 461, 557, 698, 800
BcoDI	GTCTC	3 cut(s)	214, 241, 336
BfaI	CTAG	3 cut(s)	284, 377, 669
BfmI	CTRYAG	1 cut(s)	319
BfoI	RGCGCY	2 cut(s)	234, 556
BfuAI	ACCTGC	1 cut(s)	1183
BisI	GCNGC	7 cut(s)	387, 390, 430, 443, 457, 734, 1006
BlpI	GCTNAGC	1 cut(s)	425
BlsI	GCNGC	7 cut(s)	388, 391, 431, 444, 458, 735, 1007
Bme1390I	CCNGG	9 cut(s)	175, 304, 416, 447, 461, 557, 698, 740, 800
Bme18I	GGWCC	4 cut(s)	463, 621, 637, 1076
BmeT110I	CYCGRG	4 cut(s)	467, 509, 596, 998
BmgT120I	GGNCC	7 cut(s)	463, 621, 637, 731, 796, 797, 1076
BmrFI	CCNGG	9 cut(s)	175, 304, 416, 447, 461, 557, 698, 740, 800
BmrI	ACTGGG	1 cut(s)	336
BmsI	GCATC	1 cut(s)	740
BmuI	ACTGGG	1 cut(s)	336
BoxI	GACNNNNGTC	2 cut(s)	839, 888
BpmI	CTGGAG	2 cut(s)	583, 1150
Bpu1102I	GCTNAGC	1 cut(s)	425
Bpu14I	TTCGAA	1 cut(s)	180
BpuMI	CCSGG	5 cut(s)	416, 461, 557, 698, 800
BsaBI	GATNNNNATC	4 cut(s)	156, 159, 314, 585
BsaHI	GRCGYC	5 cut(s)	231, 366, 489, 553, 602
BsaJI	CCNNGG	6 cut(s)	201, 414, 446, 466, 641, 1105
BsaWI	WCCGGW	4 cut(s)	290, 589, 605, 759
BsaXI	ACNNNNNCTCC	2 cut(s)	973, 1003
Bsc4I	CCNNNNNNNGG	8 cut(s)	472, 596, 618, 728, 729, 865, 954, 1168
Bse118I	RCCGGY	1 cut(s)	227
Bse1I	ACTGG	1 cut(s)	342
Bse21I	CCTNAGG	1 cut(s)	357
Bse8I	GATNNNNATC	4 cut(s)	156, 159, 314, 585
BseAI	TCCGGA	1 cut(s)	605
BseBI	CCWGG	4 cut(s)	175, 304, 447, 740
BseDI	CCNNGG	6 cut(s)	201, 414, 446, 466, 641, 1105
BseGI	GGATG	2 cut(s)	755, 916
BseJI	GATNNNNATC	4 cut(s)	156, 159, 314, 585
BseLI	CCNNNNNNNGG	8 cut(s)	472, 596, 618, 728, 729, 865, 954, 1168
BseMII	CTCAG	2 cut(s)	439, 1023
BseNI	ACTGG	1 cut(s)	342
BseRI	GAGGAG	2 cut(s)	202, 799
BseSI	GKGCMC	1 cut(s)	800
BseX3I	CGGCCG	2 cut(s)	457, 856
BseXI	GCAGC	3 cut(s)	373, 376, 441
Bsh1236I	CGCG	4 cut(s)	537, 643, 917, 919
Bsh1285I	CGRYCG	3 cut(s)	207, 460, 859
BshFI	GGCC	8 cut(s)	419, 445, 459, 733, 743, 798, 819, 858
BshNI	GGYRCC	2 cut(s)	230, 552
BsiEI	CGRYCG	3 cut(s)	207, 460, 859
BsiHKAI	GWGCWC	1 cut(s)	546
BsiHKCI	CYCGRG	4 cut(s)	467, 509, 596, 998
BsiWI	CGTACG	1 cut(s)	837
BslFI	GGGAC	6 cut(s)	200, 491, 499, 634, 650, 671
BslI	CCNNNNNNNGG	8 cut(s)	472, 596, 618, 728, 729, 865, 954, 1168
BsmAI	GTCTC	3 cut(s)	214, 241, 336
BsmBI	CGTCTC	2 cut(s)	214, 241
BsmFI	GGGAC	6 cut(s)	200, 491, 499, 634, 650, 671
BsnI	GGCC	8 cut(s)	419, 445, 459, 733, 743, 798, 819, 858
BsoBI	CYCGRG	4 cut(s)	467, 509, 596, 998
Bsp119I	TTCGAA	1 cut(s)	180
Bsp120I	GGGCCC	1 cut(s)	796
Bsp1286I	GDGCHC	2 cut(s)	546, 800
Bsp13I	TCCGGA	1 cut(s)	605
Bsp143I	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
Bsp1720I	GCTNAGC	1 cut(s)	425
BspANI	GGCC	8 cut(s)	419, 445, 459, 733, 743, 798, 819, 858
BspCNI	CTCAG	2 cut(s)	438, 1022
BspEI	TCCGGA	1 cut(s)	605
BspFNI	CGCG	4 cut(s)	537, 643, 917, 919
BspHI	TCATGA	1 cut(s)	346
BspMI	ACCTGC	1 cut(s)	1183
BspPI	GGATC	7 cut(s)	44, 581, 594, 603, 616, 1170, 1183
BspT104I	TTCGAA	1 cut(s)	180
BspT107I	GGYRCC	2 cut(s)	230, 552
BsrBI	CCGCTC	1 cut(s)	736
BsrFI	RCCGGY	1 cut(s)	227
BsrI	ACTGG	1 cut(s)	342
BssAI	RCCGGY	1 cut(s)	227
BssECI	CCNNGG	6 cut(s)	201, 414, 446, 466, 641, 1105
BssMI	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
BssNI	GRCGYC	5 cut(s)	231, 366, 489, 553, 602
Bst2UI	CCWGG	4 cut(s)	175, 304, 447, 740
Bst4CI	ACNGT	7 cut(s)	205, 280, 662, 832, 989, 1106, 1155
BstACI	GRCGYC	5 cut(s)	231, 366, 489, 553, 602
BstAPI	GCANNNNNTGC	1 cut(s)	1125
BstBI	TTCGAA	1 cut(s)	180
BstC8I	GCNNGC	5 cut(s)	394, 440, 551, 796, 1167
BstDEI	CTNAG	3 cut(s)	357, 425, 1009
BstDSI	CCRYGG	3 cut(s)	201, 641, 1105
BstEII	GGTNACC	1 cut(s)	692
BstF5I	GGATG	2 cut(s)	755, 916
BstFNI	CGCG	4 cut(s)	537, 643, 917, 919
BstH2I	RGCGCY	2 cut(s)	234, 556
BstHHI	GCGC	5 cut(s)	233, 537, 555, 755, 919
BstKTI	GATC	6 cut(s)	52, 157, 508, 589, 611, 1178
BstMAI	GTCTC	3 cut(s)	214, 241, 336
BstMBI	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
BstMCI	CGRYCG	3 cut(s)	207, 460, 859
BstMWI	GCNNNNNNNGC	6 cut(s)	230, 435, 541, 550, 674, 1125
BstNI	CCWGG	4 cut(s)	175, 304, 447, 740
BstNSI	RCATGY	1 cut(s)	396
BstPAI	GACNNNNGTC	2 cut(s)	839, 888
BstPI	GGTNACC	1 cut(s)	692
BstSCI	CCNGG	9 cut(s)	173, 302, 414, 445, 459, 555, 696, 738, 798
BstSFI	CTRYAG	1 cut(s)	319
BstSLI	GKGCMC	1 cut(s)	800
BstUI	CGCG	4 cut(s)	537, 643, 917, 919
BstV1I	GCAGC	3 cut(s)	373, 376, 441
BstX2I	RGATCY	3 cut(s)	586, 608, 1175
BstYI	RGATCY	3 cut(s)	586, 608, 1175
BstZI	CGGCCG	2 cut(s)	457, 856
Bsu36I	CCTNAGG	1 cut(s)	357
BsuRI	GGCC	8 cut(s)	419, 445, 459, 733, 743, 798, 819, 858
BtgI	CCRYGG	3 cut(s)	201, 641, 1105
BtsCI	GGATG	2 cut(s)	755, 916
BtsI	GCAGTG	1 cut(s)	1126
BtsIMutI	CAGTG	2 cut(s)	1126, 1160
BveI	ACCTGC	1 cut(s)	1183
Cac8I	GCNNGC	5 cut(s)	394, 440, 551, 796, 1167
CciI	TCATGA	1 cut(s)	346
CfoI	GCGC	5 cut(s)	233, 537, 555, 755, 919
Cfr10I	RCCGGY	1 cut(s)	227
Cfr13I	GGNCC	7 cut(s)	463, 621, 637, 731, 796, 797, 1076
Cfr42I	CCGCGG	1 cut(s)	644
CseI	GACGC	4 cut(s)	313, 478, 591, 923
Csp6I	GTAC	3 cut(s)	502, 838, 1158
CviAII	CATG	3 cut(s)	347, 393, 1067
CviQI	GTAC	3 cut(s)	502, 838, 1158
DdeI	CTNAG	3 cut(s)	357, 425, 1009
DinI	GGCGCC	2 cut(s)	232, 554
DpnI	GATC	6 cut(s)	51, 156, 507, 588, 610, 1177
DpnII	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
DrdI	GACNNNNNNGTC	1 cut(s)	491
DseDI	GACNNNNNNGTC	1 cut(s)	491
EaeI	YGGCCR	5 cut(s)	417, 457, 741, 817, 856
EagI	CGGCCG	2 cut(s)	457, 856
EclXI	CGGCCG	2 cut(s)	457, 856
Eco24I	GRGCYC	1 cut(s)	800
Eco32I	GATATC	1 cut(s)	16
Eco47I	GGWCC	4 cut(s)	463, 621, 637, 1076
Eco52I	CGGCCG	2 cut(s)	457, 856
Eco57I	CTGAAG	1 cut(s)	914
Eco81I	CCTNAGG	1 cut(s)	357
Eco88I	CYCGRG	4 cut(s)	467, 509, 596, 998
Eco91I	GGTNACC	1 cut(s)	692
EcoO109I	RGGNCCY	3 cut(s)	463, 621, 637
EcoO65I	GGTNACC	1 cut(s)	692
EcoRII	CCWGG	4 cut(s)	173, 302, 445, 738
EcoRV	GATATC	1 cut(s)	16
EcoT38I	GRGCYC	1 cut(s)	800
EgeI	GGCGCC	2 cut(s)	232, 554
EheI	GGCGCC	2 cut(s)	232, 554
Esp3I	CGTCTC	2 cut(s)	214, 241
FaeI	CATG	3 cut(s)	350, 396, 1070
FaiI	YATR	8 cut(s)	61, 168, 299, 348, 394, 777, 1035, 1068
FaqI	GGGAC	6 cut(s)	200, 491, 499, 634, 650, 671
FatI	CATG	3 cut(s)	346, 392, 1066
FauI	CCCGC	5 cut(s)	542, 648, 787, 912, 1162
FbaI	TGATCA	1 cut(s)	154
Fnu4HI	GCNGC	7 cut(s)	387, 390, 430, 443, 457, 734, 1006
FokI	GGATG	2 cut(s)	762, 923
FriOI	GRGCYC	1 cut(s)	800
Fsp4HI	GCNGC	7 cut(s)	387, 390, 430, 443, 457, 734, 1006
FspBI	CTAG	3 cut(s)	284, 377, 669
FspI	TGCGCA	1 cut(s)	754
GlaI	GCGC	5 cut(s)	232, 536, 554, 754, 918
GluI	GCNGC	7 cut(s)	387, 390, 430, 443, 457, 734, 1006
GsuI	CTGGAG	2 cut(s)	583, 1150
HaeII	RGCGCY	2 cut(s)	234, 556
HaeIII	GGCC	8 cut(s)	419, 445, 459, 733, 743, 798, 819, 858
HgaI	GACGC	4 cut(s)	313, 478, 591, 923
HhaI	GCGC	5 cut(s)	233, 537, 555, 755, 919
Hin1I	GRCGYC	5 cut(s)	231, 366, 489, 553, 602
Hin1II	CATG	3 cut(s)	350, 396, 1070
Hin6I	GCGC	5 cut(s)	231, 535, 553, 753, 917
HinP1I	GCGC	5 cut(s)	231, 535, 553, 753, 917
HinfI	GANTC	4 cut(s)	315, 512, 581, 686
HphI	GGTGA	2 cut(s)	1061, 1157
Hpy166II	GTNNAC	1 cut(s)	1158
Hpy188III	TCNNGA	5 cut(s)	131, 347, 509, 562, 606
Hpy8I	GTNNAC	1 cut(s)	1158
Hpy99I	CGWCG	8 cut(s)	368, 371, 488, 494, 542, 617, 620, 749
HpyAV	CCTTC	4 cut(s)	63, 635, 690, 733
HpyCH4III	ACNGT	7 cut(s)	205, 280, 662, 832, 989, 1106, 1155
HpyCH4IV	ACGT	5 cut(s)	366, 771, 836, 840, 932
HpyCH4V	TGCA	4 cut(s)	392, 411, 1066, 1192
HpyF10VI	GCNNNNNNNGC	6 cut(s)	230, 435, 541, 550, 674, 1125
HpyF3I	CTNAG	3 cut(s)	357, 425, 1009
HpySE526I	ACGT	5 cut(s)	366, 771, 836, 840, 932
Hsp92I	GRCGYC	5 cut(s)	231, 366, 489, 553, 602
Hsp92II	CATG	3 cut(s)	350, 396, 1070
HspAI	GCGC	5 cut(s)	231, 535, 553, 753, 917
KasI	GGCGCC	2 cut(s)	230, 552
KflI	GGGWCCC	2 cut(s)	621, 637
Kpn2I	TCCGGA	1 cut(s)	605
Ksp22I	TGATCA	1 cut(s)	154
KspI	CCGCGG	1 cut(s)	644
Kzo9I	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
LmnI	GCTCC	5 cut(s)	564, 682, 741, 903, 957
Lsp1109I	GCAGC	3 cut(s)	373, 376, 441
LweI	GCATC	1 cut(s)	740
MaeI	CTAG	3 cut(s)	284, 377, 669
MaeII	ACGT	5 cut(s)	366, 771, 836, 840, 932
MaeIII	GTNAC	4 cut(s)	493, 692, 832, 941
MalI	GATC	6 cut(s)	51, 156, 507, 588, 610, 1177
MbiI	CCGCTC	1 cut(s)	736
MboI	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
MboII	GAAGA	5 cut(s)	155, 319, 520, 907, 1221
MflI	RGATCY	3 cut(s)	586, 608, 1175
MhlI	GDGCHC	2 cut(s)	546, 800
MluCI	AATT	1 cut(s)	924
Mly113I	GGCGCC	2 cut(s)	231, 553
MlyI	GAGTC	1 cut(s)	521
MmeI	TCCRAC	3 cut(s)	264, 636, 727
MroI	TCCGGA	1 cut(s)	605
MroXI	GAANNNNTTC	1 cut(s)	279
MseI	TTAA	2 cut(s)	717, 852
MspA1I	CMGCKG	2 cut(s)	438, 643
MspR9I	CCNGG	9 cut(s)	175, 304, 416, 447, 461, 557, 698, 740, 800
MvaI	CCWGG	4 cut(s)	175, 304, 447, 740
MvnI	CGCG	4 cut(s)	537, 643, 917, 919
MwoI	GCNNNNNNNGC	6 cut(s)	230, 435, 541, 550, 674, 1125
NarI	GGCGCC	2 cut(s)	231, 553
NciI	CCSGG	5 cut(s)	416, 461, 557, 698, 800
NdeII	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
NlaIII	CATG	3 cut(s)	350, 396, 1070
NmuCI	GTSAC	3 cut(s)	493, 832, 941
NsbI	TGCGCA	1 cut(s)	754
NspI	RCATGY	1 cut(s)	396
NspV	TTCGAA	1 cut(s)	180
PaeI	GCATGC	1 cut(s)	396
PaeR7I	CTCGAG	1 cut(s)	509
PagI	TCATGA	1 cut(s)	346
PcsI	WCGNNNNNNNCGW	4 cut(s)	255, 489, 537, 828
PdmI	GAANNNNTTC	1 cut(s)	279
PfeI	GAWTC	3 cut(s)	315, 581, 686
Pfl23II	CGTACG	1 cut(s)	837
PflFI	GACNNNGTC	2 cut(s)	365, 488
PkrI	GCNGC	7 cut(s)	388, 391, 431, 444, 458, 735, 1007
PleI	GAGTC	1 cut(s)	520
PluTI	GGCGCC	2 cut(s)	234, 556
PpsI	GAGTC	1 cut(s)	520
PpuMI	RGGWCCY	3 cut(s)	463, 621, 637
PshAI	GACNNNNGTC	2 cut(s)	839, 888
Psp5II	RGGWCCY	3 cut(s)	463, 621, 637
Psp6I	CCWGG	4 cut(s)	173, 302, 445, 738
PspEI	GGTNACC	1 cut(s)	692
PspGI	CCWGG	4 cut(s)	173, 302, 445, 738
PspLI	CGTACG	1 cut(s)	837
PspOMI	GGGCCC	1 cut(s)	796
PspPI	GGNCC	7 cut(s)	463, 621, 637, 731, 796, 797, 1076
PspPPI	RGGWCCY	3 cut(s)	463, 621, 637
PsrI	GAACNNNNNNTAC	2 cut(s)	830, 862
PsuI	RGATCY	3 cut(s)	586, 608, 1175
PsyI	GACNNNGTC	2 cut(s)	365, 488
RsaI	GTAC	3 cut(s)	503, 839, 1159
RsaNI	GTAC	3 cut(s)	502, 838, 1158
SacII	CCGCGG	1 cut(s)	644
SaqAI	TTAA	2 cut(s)	717, 852
SatI	GCNGC	7 cut(s)	387, 390, 430, 443, 457, 734, 1006
Sau3AI	GATC	6 cut(s)	49, 154, 505, 586, 608, 1175
Sau96I	GGNCC	7 cut(s)	463, 621, 637, 731, 796, 797, 1076
SchI	GAGTC	1 cut(s)	521
ScrFI	CCNGG	9 cut(s)	175, 304, 416, 447, 461, 557, 698, 740, 800
SduI	GDGCHC	2 cut(s)	546, 800
SfaNI	GCATC	1 cut(s)	740
SfcI	CTRYAG	1 cut(s)	319
SfoI	GGCGCC	2 cut(s)	232, 554
Sfr274I	CTCGAG	1 cut(s)	509
Sfr303I	CCGCGG	1 cut(s)	644
SfuI	TTCGAA	1 cut(s)	180
SgrAI	CRCCGGYG	1 cut(s)	227
SgrBI	CCGCGG	1 cut(s)	644
SinI	GGWCC	4 cut(s)	463, 621, 637, 1076
SlaI	CTCGAG	1 cut(s)	509
SmlI	CTYRAG	1 cut(s)	509
SmoI	CTYRAG	1 cut(s)	509
SphI	GCATGC	1 cut(s)	396
Sse9I	AATT	1 cut(s)	924
SspDI	GGCGCC	2 cut(s)	230, 552
SspMI	CTAG	3 cut(s)	284, 377, 669
StyD4I	CCNGG	9 cut(s)	173, 302, 414, 445, 459, 555, 696, 738, 798
TaaI	ACNGT	7 cut(s)	205, 280, 662, 832, 989, 1106, 1155
TaiI	ACGT	5 cut(s)	369, 774, 839, 843, 935
TaqI	TCGA	7 cut(s)	180, 258, 264, 510, 531, 570, 863
TasI	AATT	1 cut(s)	924
TauI	GCSGC	4 cut(s)	445, 459, 736, 1008
TfiI	GAWTC	3 cut(s)	315, 581, 686
Tru1I	TTAA	2 cut(s)	717, 852
Tru9I	TTAA	2 cut(s)	717, 852
TscAI	CASTG	2 cut(s)	1126, 1160
TseFI	GTSAC	3 cut(s)	493, 832, 941
TseI	GCWGC	3 cut(s)	386, 389, 429
Tsp45I	GTSAC	3 cut(s)	493, 832, 941
TspDTI	ATGAA	3 cut(s)	34, 155, 363
TspGWI	ACGGA	1 cut(s)	512
TspRI	CASTG	2 cut(s)	1126, 1160
Tth111I	GACNNNGTC	2 cut(s)	365, 488
VpaK11BI	GGWCC	4 cut(s)	463, 621, 637, 1076
XapI	RAATTY	1 cut(s)	924
XceI	RCATGY	1 cut(s)	396
XhoI	CTCGAG	1 cut(s)	509
XmnI	GAANNNNTTC	1 cut(s)	279
XspI	CTAG	3 cut(s)	284, 377, 669
ZraI	GACGTC	1 cut(s)	367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.