Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0014091

Physical Location & Seq

Genomic Coordinates Forward (+)

25207350 .. 25208561

Gene Structure 1212 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g24530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1212 bp

ATGAACAACTTCTCCTCAGGATTCCATTTCCCAGCCCAAAAAAGAATTTCAGAAGAAGAAAGAGAAAGCACAGAGGAAGATAAACTACAAGAAGAAGAAGGAAAAGGATCAGAGTTCAACAATTGTGACTGGGATTTCAGTCTCTCAACAGTTGTGTCCTCCACCGCCTGCCGCGGCGCCGCGTCCAACACCCTTGGCGTAATCGAGTTCGACCCATCAAACACCGTCTTAGCCACCGGCGGAATCGCCAGAAAAATAAGAGGTTACAGCTTGAAATCTCTCTTACCCAATTATGATAATCAAATCTGTGGAAGTTCTAGTAGTATGGTGGATTTGATAGACCACGTCGATGCATGCGACTACACCATTTGCACTCCAGCCAAGCTCAGCAGCCTCCGCTGGCGCCCCGGATCCGACGGCCGGGTCCTGGCCTCCGGCGACTATGATGGCGTCGTCACGGAATACGACCTCGAAACAAAACTACCCATCTTCGAGCGCGACGAGCACGGCGGGCGCAGGGTCTGGAGCGTGGATTATTCGCATTCCAATCCGGTTCTCGGAGCGTCTGCCTCCGACGACGGGACCCTCCAGCTGTGGGACCCGCGCCACGAGGGCGGGAATTGCGTGGCCAGTGTGCAGCCCAGCGACGCGCGAAATCCGGTCTGCTGCGTGGAGTTTGATCCCTTCGGCGGGGAGCTTCTGGCCGTTGGATGCGCGGACCGGAAGGCCTACGGCTACGATGTTCGGAAAATGGTGAGCCCGGTATTGGTCTTCGACGGACACAGGAAAACCGTGACGTACGTGCGGTTTCTTGACGGGCGTACGGTGGTAACAGCTGGCACGGACGGGTGTTTGAAGCTGTGGGACACGGAGGATTCGCGGGCGATTCGGACGTACAAGGGGCACGCCAACTGTCGGAGCTTTGTGGGGTTATCGGTGTGGAGAAATGGAGGGCTAATCGGGTGCGGGTCGGAAAATAACCGGGTTTTCGTGTACGATAAGAGGTGGGGCGAGCCCATTTGGGTGCACGGGTTTGGGGAGGTGGGTGCAGGTGGTAGTAATCGCGGCGGGTGCGGTGAGGACTTGGGCGCGTTTGTAAGTAGCGTGTGCTGGCGGCAAGTTGGTGAGGAGCAGTGCACGCTCGTGGCGGGTGGGTCGGACGGGGTTTTGCAGGTCTTTTTGGGAAGGAGGAAGTCCAATCTTGTCGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009657 GO:0009658 GO:0009791 GO:0009812 GO:0009987 GO:0010114 GO:0010119 GO:0010218 GO:0010224 GO:0010228 GO:0016043 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043393 GO:0043412 GO:0043496 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051098 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40

Protein Analysis

404

Amino Acids

43.84

Weight (kDa)

5.17

Isoelectric Point (pI)

45.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	126 - 266	2.5e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	128 - 334	1.6e-22	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	128 - 251	3e-10	THOC3 beta-propeller domain
WD40_Prp19	PF24814	128 - 271	9.2e-12	Prp19 WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	138 - 340	2.7e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	142 - 271	2.6e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	171 - 334	2.2e-09	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	185 - 323	1.8e-06	G protein beta WD-40 repeat protein
Beta-prop_EIPR1	PF23609	188 - 288	7.8e-07	EIPR1 beta-propeller
Beta-prop_TEP1_2nd	PF25047	217 - 307	6e-12	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	218 - 333	4.2e-11	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	219 - 309	8.9e-07	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	220 - 336	1.8e-09	THOC3 beta-propeller domain
Beta-prop_DCAF4	PF23761	261 - 320	5.8e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40_CDC20-Fz	PF24807	263 - 339	1.2e-06	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23730
fragaria_vesca	FvH4_7g29370
malus_domestica	MD01G1199700.v1.1 MD07G1267700.v1.1
prunus_persica	Prupe.2G293400_v2.0.a1
pyrus_communis	pycom01g20980 pycom07g24530
rosa_chinensis	RchiOBHm_Chr1g0377161
rosa_laevigata	RLG00000026540
rosa_multiflora	Rmu_ssc0000155.1_g000016
rosa_roxburghii	Rroxscaffold_4G00281420
rosa_rugosa	Rorug01G0401700
rosa_samantha	Rh1AG419100 Rh1BG378200 Rh1CG392200 Rh1DG408900
rosa_wichuraiana	Rw1G036760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1040
AasI	GACNNNNNNGTC	1 cut(s)	422
Acc36I	ACCTGC	2 cut(s)	1040, 1162
AccB1I	GGYRCC	2 cut(s)	176, 402
AclWI	GGATC	4 cut(s)	115, 405, 418, 674
AcoI	YGGCCR	3 cut(s)	418, 627, 702
AcsI	RAATTY	1 cut(s)	45
AcyI	GRCGYC	3 cut(s)	177, 403, 450
AfaI	GTAC	4 cut(s)	800, 823, 896, 995
AfiI	CCNNNNNNNGG	9 cut(s)	420, 427, 557, 579, 595, 689, 690, 766, 915
AgsI	TTSAA	3 cut(s)	118, 274, 856
AjiI	CACGTC	1 cut(s)	346
AjnI	CCWGG	1 cut(s)	426
AleI	CACNNNNGTG	1 cut(s)	1142
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	7 cut(s)	270, 385, 592, 697, 836, 859, 921
AluI	AGCT	7 cut(s)	270, 385, 592, 697, 836, 859, 921
Alw21I	GWGCWC	3 cut(s)	507, 1029, 1139
Alw26I	GTCTC	1 cut(s)	146
Alw44I	GTGCAC	2 cut(s)	1025, 1135
AlwI	GGATC	4 cut(s)	115, 405, 418, 674
AlwNI	CAGNNNCTG	1 cut(s)	522
AoxI	GGCC	5 cut(s)	418, 429, 627, 702, 726
ApaLI	GTGCAC	2 cut(s)	1025, 1135
ApeKI	GCWGC	3 cut(s)	390, 637, 666
ApoI	RAATTY	1 cut(s)	45
Asp700I	GAANNNNTTC	1 cut(s)	8
AspLEI	GCGC	8 cut(s)	179, 405, 498, 516, 606, 652, 716, 1091
AspS9I	GGNCC	4 cut(s)	424, 582, 598, 718
AsuC2I	CCSGG	4 cut(s)	408, 422, 761, 983
AsuHPI	GGTGA	3 cut(s)	766, 1088, 1136
AvaII	GGWCC	4 cut(s)	424, 582, 598, 718
AxyI	CCTNAGG	1 cut(s)	16
BaeGI	GKGCMC	3 cut(s)	906, 1029, 1139
BalI	TGGCCA	1 cut(s)	629
BamHI	GGATCC	1 cut(s)	410
BanI	GGYRCC	2 cut(s)	176, 402
BanII	GRGCYC	2 cut(s)	761, 1017
BarI	GAAGNNNNNNTAC	2 cut(s)	69, 101
BauI	CACGAG	2 cut(s)	608, 1142
BbsI	GAAGAC	1 cut(s)	763
Bbv12I	GWGCWC	3 cut(s)	507, 1029, 1139
BbvI	GCAGC	3 cut(s)	402, 649, 653
BccI	CCATC	3 cut(s)	223, 440, 494
BceAI	ACGGC	4 cut(s)	433, 523, 689, 748
BciT130I	CCWGG	1 cut(s)	428
BcnI	CCSGG	4 cut(s)	408, 422, 761, 983
BcoDI	GTCTC	1 cut(s)	146
BfaI	CTAG	1 cut(s)	318
BfoI	RGCGCY	2 cut(s)	180, 406
BfuAI	ACCTGC	2 cut(s)	1040, 1162
BglI	GCCNNNNNGGC	1 cut(s)	612
BisI	GCNGC	8 cut(s)	172, 175, 180, 391, 638, 667, 1066, 1115
BlpI	GCTNAGC	1 cut(s)	386
BlsI	GCNGC	8 cut(s)	173, 176, 181, 392, 639, 668, 1067, 1116
Bme1390I	CCNGG	5 cut(s)	408, 422, 428, 761, 983
Bme18I	GGWCC	4 cut(s)	424, 582, 598, 718
BmgBI	CACGTC	1 cut(s)	346
BmgT120I	GGNCC	4 cut(s)	424, 582, 598, 718
BmiI	GGNNCC	8 cut(s)	178, 404, 412, 425, 583, 584, 599, 600
BmrFI	CCNGG	5 cut(s)	408, 422, 428, 761, 983
BmrI	ACTGGG	1 cut(s)	139
BmsI	GCATC	2 cut(s)	340, 701
BmuI	ACTGGG	1 cut(s)	139
BpiI	GAAGAC	1 cut(s)	763
BpmI	CTGGAG	3 cut(s)	360, 544, 572
Bpu1102I	GCTNAGC	1 cut(s)	386
BpuMI	CCSGG	4 cut(s)	408, 422, 761, 983
BsaAI	YACGTR	1 cut(s)	802
BsaHI	GRCGYC	3 cut(s)	177, 403, 450
BsaJI	CCNNGG	3 cut(s)	172, 193, 406
BsaWI	WCCGGW	3 cut(s)	550, 658, 720
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	9 cut(s)	420, 427, 557, 579, 595, 689, 690, 766, 915
Bse118I	RCCGGY	1 cut(s)	236
Bse1I	ACTGG	2 cut(s)	134, 630
Bse21I	CCTNAGG	1 cut(s)	16
BseBI	CCWGG	1 cut(s)	428
BseDI	CCNNGG	3 cut(s)	172, 193, 406
BseGI	GGATG	1 cut(s)	716
BseLI	CCNNNNNNNGG	9 cut(s)	420, 427, 557, 579, 595, 689, 690, 766, 915
BseMII	CTCAG	2 cut(s)	30, 400
BseNI	ACTGG	2 cut(s)	134, 630
BseRI	GAGGAG	2 cut(s)	4, 1142
BseSI	GKGCMC	3 cut(s)	906, 1029, 1139
BseX3I	CGGCCG	1 cut(s)	418
BseXI	GCAGC	3 cut(s)	402, 649, 653
BseYI	CCCAGC	2 cut(s)	31, 641
BsgI	GTGCAG	2 cut(s)	656, 1068
Bsh1285I	CGRYCG	1 cut(s)	421
BshFI	GGCC	5 cut(s)	420, 431, 629, 704, 728
BshNI	GGYRCC	2 cut(s)	176, 402
BsiEI	CGRYCG	1 cut(s)	421
BsiHKAI	GWGCWC	3 cut(s)	507, 1029, 1139
BsiSI	CCGG	9 cut(s)	237, 408, 421, 435, 551, 659, 721, 761, 982
BsiWI	CGTACG	2 cut(s)	798, 821
BslFI	GGGAC	3 cut(s)	595, 611, 878
BslI	CCNNNNNNNGG	9 cut(s)	420, 427, 557, 579, 595, 689, 690, 766, 915
BsmAI	GTCTC	1 cut(s)	146
BsmFI	GGGAC	3 cut(s)	595, 611, 878
BsmI	GAATGC	1 cut(s)	541
BsnI	GGCC	5 cut(s)	420, 431, 629, 704, 728
Bsp1286I	GDGCHC	6 cut(s)	507, 761, 906, 1017, 1029, 1139
Bsp143I	GATC	3 cut(s)	107, 410, 679
Bsp1720I	GCTNAGC	1 cut(s)	386
BspANI	GGCC	5 cut(s)	420, 431, 629, 704, 728
BspCNI	CTCAG	2 cut(s)	29, 399
BspLI	GGNNCC	8 cut(s)	178, 404, 412, 425, 583, 584, 599, 600
BspMI	ACCTGC	2 cut(s)	1040, 1162
BspPI	GGATC	4 cut(s)	115, 405, 418, 674
BspT107I	GGYRCC	2 cut(s)	176, 402
BsrFI	RCCGGY	1 cut(s)	236
BsrI	ACTGG	2 cut(s)	134, 630
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	3 cut(s)	172, 193, 406
BssMI	GATC	3 cut(s)	107, 410, 679
BssNI	GRCGYC	3 cut(s)	177, 403, 450
BssSI	CACGAG	2 cut(s)	608, 1142
BssT1I	CCWWGG	1 cut(s)	193
Bst2BI	CACGAG	2 cut(s)	608, 1142
Bst2UI	CCWGG	1 cut(s)	428
Bst4CI	ACNGT	5 cut(s)	151, 226, 793, 826, 914
BstACI	GRCGYC	3 cut(s)	177, 403, 450
BstBAI	YACGTR	1 cut(s)	802
BstDEI	CTNAG	3 cut(s)	16, 229, 386
BstDSI	CCRYGG	1 cut(s)	172
BstF5I	GGATG	1 cut(s)	716
BstH2I	RGCGCY	2 cut(s)	180, 406
BstHHI	GCGC	8 cut(s)	179, 405, 498, 516, 606, 652, 716, 1091
BstKTI	GATC	3 cut(s)	110, 413, 682
BstMAI	GTCTC	1 cut(s)	146
BstMBI	GATC	3 cut(s)	107, 410, 679
BstMCI	CGRYCG	1 cut(s)	421
BstMWI	GCNNNNNNNGC	7 cut(s)	396, 502, 511, 612, 621, 1071, 1138
BstNI	CCWGG	1 cut(s)	428
BstNSI	RCATGY	1 cut(s)	357
BstSCI	CCNGG	5 cut(s)	406, 420, 426, 759, 981
BstSLI	GKGCMC	3 cut(s)	906, 1029, 1139
BstV1I	GCAGC	3 cut(s)	402, 649, 653
BstV2I	GAAGAC	1 cut(s)	763
BstX2I	RGATCY	1 cut(s)	410
BstYI	RGATCY	1 cut(s)	410
BstZI	CGGCCG	1 cut(s)	418
Bsu36I	CCTNAGG	1 cut(s)	16
BsuRI	GGCC	5 cut(s)	420, 431, 629, 704, 728
BtgI	CCRYGG	1 cut(s)	172
BtrI	CACGTC	1 cut(s)	346
BtsCI	GGATG	1 cut(s)	716
BtsI	GCAGTG	1 cut(s)	1139
BtsIMutI	CAGTG	2 cut(s)	637, 1139
BveI	ACCTGC	2 cut(s)	1040, 1162
CaiI	CAGNNNCTG	1 cut(s)	522
CfoI	GCGC	8 cut(s)	179, 405, 498, 516, 606, 652, 716, 1091
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	4 cut(s)	424, 582, 598, 718
Cfr42I	CCGCGG	1 cut(s)	175
CpoI	CGGWCCG	1 cut(s)	718
CseI	GACGC	4 cut(s)	171, 439, 552, 656
Csp6I	GTAC	4 cut(s)	799, 822, 895, 994
CspI	CGGWCCG	1 cut(s)	718
CviAII	CATG	1 cut(s)	354
CviQI	GTAC	4 cut(s)	799, 822, 895, 994
DdeI	CTNAG	3 cut(s)	16, 229, 386
DinI	GGCGCC	2 cut(s)	178, 404
DpnI	GATC	3 cut(s)	109, 412, 681
DpnII	GATC	3 cut(s)	107, 410, 679
DrdI	GACNNNNNNGTC	1 cut(s)	422
DseDI	GACNNNNNNGTC	1 cut(s)	422
EaeI	YGGCCR	3 cut(s)	418, 627, 702
EagI	CGGCCG	1 cut(s)	418
EciI	GGCGGA	1 cut(s)	255
EclXI	CGGCCG	1 cut(s)	418
Eco130I	CCWWGG	1 cut(s)	193
Eco147I	AGGCCT	1 cut(s)	728
Eco24I	GRGCYC	2 cut(s)	761, 1017
Eco47I	GGWCC	4 cut(s)	424, 582, 598, 718
Eco52I	CGGCCG	1 cut(s)	418
Eco81I	CCTNAGG	1 cut(s)	16
EcoO109I	RGGNCCY	3 cut(s)	424, 582, 598
EcoRII	CCWGG	1 cut(s)	426
EcoT14I	CCWWGG	1 cut(s)	193
EcoT22I	ATGCAT	1 cut(s)	355
EcoT38I	GRGCYC	2 cut(s)	761, 1017
EgeI	GGCGCC	2 cut(s)	178, 404
EheI	GGCGCC	2 cut(s)	178, 404
ErhI	CCWWGG	1 cut(s)	193
FaeI	CATG	1 cut(s)	357
FaiI	YATR	4 cut(s)	294, 326, 355, 444
FaqI	GGGAC	3 cut(s)	595, 611, 878
FatI	CATG	1 cut(s)	353
FauI	CCCGC	8 cut(s)	503, 608, 609, 683, 873, 959, 1061, 1141
Fnu4HI	GCNGC	8 cut(s)	172, 175, 180, 391, 638, 667, 1066, 1115
FokI	GGATG	1 cut(s)	723
FriOI	GRGCYC	2 cut(s)	761, 1017
Fsp4HI	GCNGC	8 cut(s)	172, 175, 180, 391, 638, 667, 1066, 1115
FspBI	CTAG	1 cut(s)	318
GlaI	GCGC	8 cut(s)	178, 404, 497, 515, 605, 651, 715, 1090
GluI	GCNGC	8 cut(s)	172, 175, 180, 391, 638, 667, 1066, 1115
GsaI	CCCAGC	2 cut(s)	35, 645
GsuI	CTGGAG	3 cut(s)	360, 544, 572
HaeII	RGCGCY	2 cut(s)	180, 406
HaeIII	GGCC	5 cut(s)	420, 431, 629, 704, 728
HapII	CCGG	9 cut(s)	237, 408, 421, 435, 551, 659, 721, 761, 982
HgaI	GACGC	4 cut(s)	171, 439, 552, 656
HhaI	GCGC	8 cut(s)	179, 405, 498, 516, 606, 652, 716, 1091
Hin1I	GRCGYC	3 cut(s)	177, 403, 450
Hin1II	CATG	1 cut(s)	357
Hin6I	GCGC	8 cut(s)	177, 403, 496, 514, 604, 650, 714, 1089
HinP1I	GCGC	8 cut(s)	177, 403, 496, 514, 604, 650, 714, 1089
HinfI	GANTC	4 cut(s)	21, 243, 875, 886
HpaII	CCGG	9 cut(s)	237, 408, 421, 435, 551, 659, 721, 761, 982
HphI	GGTGA	3 cut(s)	766, 1088, 1136
Hpy166II	GTNNAC	3 cut(s)	994, 1027, 1137
Hpy188III	TCNNGA	3 cut(s)	18, 523, 812
Hpy8I	GTNNAC	3 cut(s)	994, 1027, 1137
Hpy99I	CGWCG	8 cut(s)	350, 419, 455, 503, 578, 581, 650, 779
HpyAV	CCTTC	4 cut(s)	92, 694, 718, 1179
HpyCH4III	ACNGT	5 cut(s)	151, 226, 793, 826, 914
HpyCH4IV	ACGT	4 cut(s)	345, 797, 801, 893
HpyCH4V	TGCA	7 cut(s)	353, 372, 637, 1027, 1049, 1137, 1171
HpyF10VI	GCNNNNNNNGC	7 cut(s)	396, 502, 511, 612, 621, 1071, 1138
HpyF3I	CTNAG	3 cut(s)	16, 229, 386
HpySE526I	ACGT	4 cut(s)	345, 797, 801, 893
Hsp92I	GRCGYC	3 cut(s)	177, 403, 450
Hsp92II	CATG	1 cut(s)	357
HspAI	GCGC	8 cut(s)	177, 403, 496, 514, 604, 650, 714, 1089
KasI	GGCGCC	2 cut(s)	176, 402
KflI	GGGWCCC	2 cut(s)	582, 598
KspI	CCGCGG	1 cut(s)	175
Kzo9I	GATC	3 cut(s)	107, 410, 679
LmnI	GCTCC	5 cut(s)	525, 560, 694, 918, 1129
Lsp1109I	GCAGC	3 cut(s)	402, 649, 653
LweI	GCATC	2 cut(s)	340, 701
MaeI	CTAG	1 cut(s)	318
MaeII	ACGT	4 cut(s)	345, 797, 801, 893
MaeIII	GTNAC	5 cut(s)	125, 263, 454, 793, 829
MalI	GATC	3 cut(s)	109, 412, 681
MboI	GATC	3 cut(s)	107, 410, 679
MboII	GAAGA	7 cut(s)	65, 68, 89, 104, 107, 481, 763
MfeI	CAATTG	1 cut(s)	121
MflI	RGATCY	1 cut(s)	410
MhlI	GDGCHC	6 cut(s)	507, 761, 906, 1017, 1029, 1139
MlsI	TGGCCA	1 cut(s)	629
MluCI	AATT	4 cut(s)	45, 121, 289, 619
MluNI	TGGCCA	1 cut(s)	629
Mly113I	GGCGCC	2 cut(s)	177, 403
MmeI	TCCRAC	8 cut(s)	210, 438, 597, 688, 896, 951, 1137, 1186
Mox20I	TGGCCA	1 cut(s)	629
Mph1103I	ATGCAT	1 cut(s)	355
MroXI	GAANNNNTTC	1 cut(s)	8
MscI	TGGCCA	1 cut(s)	629
MslI	CAYNNNNRTG	3 cut(s)	348, 1022, 1142
Msp20I	TGGCCA	1 cut(s)	629
MspA1I	CMGCKG	4 cut(s)	174, 399, 592, 836
MspI	CCGG	9 cut(s)	237, 408, 421, 435, 551, 659, 721, 761, 982
MspR9I	CCNGG	5 cut(s)	408, 422, 428, 761, 983
MunI	CAATTG	1 cut(s)	121
Mva1269I	GAATGC	1 cut(s)	541
MvaI	CCWGG	1 cut(s)	428
MwoI	GCNNNNNNNGC	7 cut(s)	396, 502, 511, 612, 621, 1071, 1138
NarI	GGCGCC	2 cut(s)	177, 403
NciI	CCSGG	4 cut(s)	408, 422, 761, 983
NdeII	GATC	3 cut(s)	107, 410, 679
NlaIII	CATG	1 cut(s)	357
NlaIV	GGNNCC	8 cut(s)	178, 404, 412, 425, 583, 584, 599, 600
NmuCI	GTSAC	3 cut(s)	125, 454, 793
NsiI	ATGCAT	1 cut(s)	355
NspI	RCATGY	1 cut(s)	357
OliI	CACNNNNGTG	1 cut(s)	1142
PaeI	GCATGC	1 cut(s)	357
PaqCI	CACCTGC	1 cut(s)	1040
PceI	AGGCCT	1 cut(s)	728
PcsI	WCGNNNNNNNCGW	2 cut(s)	354, 498
PctI	GAATGC	1 cut(s)	541
PdmI	GAANNNNTTC	1 cut(s)	8
PfeI	GAWTC	4 cut(s)	21, 243, 875, 886
Pfl23II	CGTACG	2 cut(s)	798, 821
PflFI	GACNNNGTC	1 cut(s)	344
PkrI	GCNGC	8 cut(s)	173, 176, 181, 392, 639, 668, 1067, 1116
PluTI	GGCGCC	2 cut(s)	180, 406
Ppu21I	YACGTR	1 cut(s)	802
PpuMI	RGGWCCY	3 cut(s)	424, 582, 598
Psp5II	RGGWCCY	3 cut(s)	424, 582, 598
Psp6I	CCWGG	1 cut(s)	426
PspFI	CCCAGC	2 cut(s)	31, 641
PspGI	CCWGG	1 cut(s)	426
PspLI	CGTACG	2 cut(s)	798, 821
PspN4I	GGNNCC	8 cut(s)	178, 404, 412, 425, 583, 584, 599, 600
PspPI	GGNCC	4 cut(s)	424, 582, 598, 718
PspPPI	RGGWCCY	3 cut(s)	424, 582, 598
PstNI	CAGNNNCTG	1 cut(s)	522
PsuI	RGATCY	1 cut(s)	410
PsyI	GACNNNGTC	1 cut(s)	344
PvuII	CAGCTG	2 cut(s)	592, 836
RsaI	GTAC	4 cut(s)	800, 823, 896, 995
RsaNI	GTAC	4 cut(s)	799, 822, 895, 994
RseI	CAYNNNNRTG	3 cut(s)	348, 1022, 1142
Rsr2I	CGGWCCG	1 cut(s)	718
RsrII	CGGWCCG	1 cut(s)	718
SacII	CCGCGG	1 cut(s)	175
SatI	GCNGC	8 cut(s)	172, 175, 180, 391, 638, 667, 1066, 1115
Sau3AI	GATC	3 cut(s)	107, 410, 679
Sau96I	GGNCC	4 cut(s)	424, 582, 598, 718
ScrFI	CCNGG	5 cut(s)	408, 422, 428, 761, 983
SduI	GDGCHC	6 cut(s)	507, 761, 906, 1017, 1029, 1139
SfaNI	GCATC	2 cut(s)	340, 701
SfoI	GGCGCC	2 cut(s)	178, 404
Sfr303I	CCGCGG	1 cut(s)	175
SgrAI	CRCCGGYG	1 cut(s)	236
SgrBI	CCGCGG	1 cut(s)	175
SinI	GGWCC	4 cut(s)	424, 582, 598, 718
SmiMI	CAYNNNNRTG	3 cut(s)	348, 1022, 1142
SphI	GCATGC	1 cut(s)	357
Sse9I	AATT	4 cut(s)	45, 121, 289, 619
SseBI	AGGCCT	1 cut(s)	728
SspDI	GGCGCC	2 cut(s)	176, 402
SspMI	CTAG	1 cut(s)	318
StuI	AGGCCT	1 cut(s)	728
StyD4I	CCNGG	5 cut(s)	406, 420, 426, 759, 981
StyI	CCWWGG	1 cut(s)	193
TaaI	ACNGT	5 cut(s)	151, 226, 793, 826, 914
TaiI	ACGT	4 cut(s)	348, 800, 804, 896
TaqI	TCGA	6 cut(s)	204, 210, 348, 471, 492, 774
TasI	AATT	4 cut(s)	45, 121, 289, 619
TauI	GCSGC	5 cut(s)	174, 177, 182, 1068, 1117
TfiI	GAWTC	4 cut(s)	21, 243, 875, 886
TscAI	CASTG	2 cut(s)	637, 1139
TseFI	GTSAC	3 cut(s)	125, 454, 793
TseI	GCWGC	3 cut(s)	390, 637, 666
Tsp45I	GTSAC	3 cut(s)	125, 454, 793
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	4 cut(s)	473, 792, 857, 884
TspRI	CASTG	2 cut(s)	637, 1139
Tth111I	GACNNNGTC	1 cut(s)	344
VneI	GTGCAC	2 cut(s)	1025, 1135
VpaK11BI	GGWCC	4 cut(s)	424, 582, 598, 718
XapI	RAATTY	1 cut(s)	45
XceI	RCATGY	1 cut(s)	357
XmnI	GAANNNNTTC	1 cut(s)	8
XspI	CTAG	1 cut(s)	318
Zsp2I	ATGCAT	1 cut(s)	355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.