Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014091

Physical Location & Seq

Genomic Coordinates Forward (+)

33550835 .. 33552049

Gene Structure 1215 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1267700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

ATGAACAACTTCTCCTCAGGATTCCATTTCCCAGCCCAACAAAGAATTTCAGAAGAAGAAAGAGAAAGCACAGAAGAAGATAAGCTACAAGAAGAAGAAGGAAAAGGATCAGAGTTCAACAATTGTGACTGGGATTTCAGTCTCTCAACAGTTGTGTCCTCCACTGCCTGCCGCGGCGCCGCGTCCAACACCCTTGGCGTAATCGAGTTCGACCCATCAAACACCGTCTTAGCCACCGGCGGAATCGCCAGAAAAATAAGAGGTTACAGCTTGAAATCTCTCTTACCCAATTATGATAATCAAATCTGTGGAAGTTCTAGTGATAGTATGGCGGATTTGATAGACCACGTCGATGCATGCGACTACACCATTTGCACTCCCGCCAAGCTCAGCAGCCTCCGCTGGCGCCCCGGATCCGACGGCCGGGTCCTGGCCTCCGGCGACTATGATGGCGTCGTCACGGAATACGACCTCGAAACAAAACTACCCATCTTCGAGCGCGACGAGCACGGCGGGCGCAGGGTCTGGAGCGTGGATTATTCGCATTCCGATCCGGTTCTCGGAGCGTCTGCCTCCGACGACGGGACCCTCCAGCTGTGGGACCCGCGCCACGAGGGCGGCAATTGCGTGGCCAGTGTGCAGCCCAGCGACGCTCGAAATCCGGTCTGTTGCGTGGAGTTTGATCCCTTCGGCGGGGCCCTTCTGGCCGTTGGATGCGCGGACCGGAAGGCCTACGGCTACGATGTTCGGAAAATGGTGAGCCCGGTTTCGGTCTTCGACGGACACAGGAAAACCGTGACGTACGTGCGGTTTCTTAACGGGCGTACGGTGGTAACAGCTGGCACGGACGGGTGTTTGAAGCTGTGGGACACGGAGGATTCGCGGGCGATTCGGACGTACAAGGGGCACGCCAATAGTCGGAGCTTTGTGGGGTTATCGGTGTGGAGAAATGGGGAGCTAATCGGGTGCGGGTCGGAAAATAACCGGGTTTTCGTGTACGATAAGAGGTGGGGCGAGCCCATTTGGGTGCACGGGTTTGGGGAGGTGGGTGCAGGAAGTAGTAATAGCGGTGGGTGCGGTGAGGACTTGGGCGCGTTTGTAAGTAGCGTGTGCTGGCGGCAAGTTGGTGAGGAGCAGTGCACGCTCGTGGCGGGTGGGTCGGATGGGGTTTTGCAGGTTTTTTTGGGAAGGAGGAAGTCCATTCTAGTCGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009657 GO:0009658 GO:0009791 GO:0009812 GO:0009987 GO:0010114 GO:0010119 GO:0010218 GO:0010224 GO:0010228 GO:0016043 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043393 GO:0043412 GO:0043496 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051098 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40

Protein Analysis

405

Amino Acids

43.86

Weight (kDa)

4.99

Isoelectric Point (pI)

46.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	102 - 335	1.2e-24	WDR5 beta-propeller domain
WD40_Prp19	PF24814	105 - 272	1.1e-11	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	128 - 267	1.1e-09	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	129 - 252	9.8e-10	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	139 - 341	1.8e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	143 - 271	1.8e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	172 - 336	3.7e-10	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	183 - 336	1.4e-06	G protein beta WD-40 repeat protein
Beta-prop_EIPR1	PF23609	187 - 289	4e-07	EIPR1 beta-propeller
Beta-prop_WDR3_1st	PF25173	189 - 334	4.5e-14	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	219 - 309	2.6e-11	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	220 - 310	1.4e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	221 - 335	1.8e-06	WDR3 second beta-propeller domain
Beta-prop_DCAF4	PF23761	236 - 317	7.5e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_THOC3	PF25174	254 - 340	5.2e-08	THOC3 beta-propeller domain
WD40_Prp19	PF24814	257 - 335	4.3e-06	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	264 - 339	3.2e-07	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014091)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23730
fragaria_vesca	FvH4_7g29370
malus_domestica	MD01G1199700.v1.1 MD07G1267700.v1.1
prunus_persica	Prupe.2G293400_v2.0.a1
pyrus_communis	pycom01g20980 pycom07g24530
rosa_chinensis	RchiOBHm_Chr1g0377161
rosa_laevigata	RLG00000026540
rosa_multiflora	Rmu_ssc0000155.1_g000016
rosa_roxburghii	Rroxscaffold_4G00281420
rosa_rugosa	Rorug01G0401700
rosa_samantha	Rh1AG419100 Rh1BG378200 Rh1CG392200 Rh1DG408900
rosa_wichuraiana	Rw1G036760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	425
Acc36I	ACCTGC	1 cut(s)	1165
AccB1I	GGYRCC	2 cut(s)	176, 405
AccII	CGCG	7 cut(s)	174, 182, 501, 607, 719, 883, 1094
AclWI	GGATC	5 cut(s)	115, 408, 421, 545, 677
AcoI	YGGCCR	3 cut(s)	421, 630, 705
AcsI	RAATTY	1 cut(s)	45
AcyI	GRCGYC	3 cut(s)	177, 406, 453
AfaI	GTAC	4 cut(s)	803, 826, 899, 998
AfiI	CCNNNNNNNGG	9 cut(s)	423, 430, 560, 582, 598, 692, 693, 769, 918
AgsI	TTSAA	3 cut(s)	118, 274, 859
AjiI	CACGTC	1 cut(s)	349
AjnI	CCWGG	1 cut(s)	429
AleI	CACNNNNGTG	1 cut(s)	1145
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	8 cut(s)	85, 270, 388, 595, 839, 862, 924, 958
AluI	AGCT	8 cut(s)	85, 270, 388, 595, 839, 862, 924, 958
Alw21I	GWGCWC	3 cut(s)	510, 1032, 1142
Alw26I	GTCTC	1 cut(s)	146
Alw44I	GTGCAC	2 cut(s)	1028, 1138
AlwI	GGATC	5 cut(s)	115, 408, 421, 545, 677
AlwNI	CAGNNNCTG	1 cut(s)	525
AoxI	GGCC	6 cut(s)	421, 432, 630, 696, 705, 729
ApaI	GGGCCC	1 cut(s)	700
ApaLI	GTGCAC	2 cut(s)	1028, 1138
ApeKI	GCWGC	2 cut(s)	393, 640
ApoI	RAATTY	1 cut(s)	45
Asp700I	GAANNNNTTC	1 cut(s)	8
AspLEI	GCGC	7 cut(s)	179, 408, 501, 519, 609, 719, 1094
AspS9I	GGNCC	6 cut(s)	427, 585, 601, 696, 697, 721
AsuC2I	CCSGG	4 cut(s)	411, 425, 764, 986
AsuHPI	GGTGA	3 cut(s)	769, 1091, 1139
AvaII	GGWCC	4 cut(s)	427, 585, 601, 721
AxyI	CCTNAGG	1 cut(s)	16
BaeGI	GKGCMC	4 cut(s)	700, 909, 1032, 1142
BalI	TGGCCA	1 cut(s)	632
BamHI	GGATCC	1 cut(s)	413
BanI	GGYRCC	2 cut(s)	176, 405
BanII	GRGCYC	3 cut(s)	700, 764, 1020
BarI	GAAGNNNNNNTAC	2 cut(s)	69, 101
BauI	CACGAG	2 cut(s)	611, 1145
BbsI	GAAGAC	1 cut(s)	766
Bbv12I	GWGCWC	3 cut(s)	510, 1032, 1142
BbvI	GCAGC	2 cut(s)	405, 652
BccI	CCATC	4 cut(s)	223, 443, 497, 1157
BceAI	ACGGC	4 cut(s)	436, 526, 692, 751
BciT130I	CCWGG	1 cut(s)	431
BcnI	CCSGG	4 cut(s)	411, 425, 764, 986
BcoDI	GTCTC	1 cut(s)	146
BfaI	CTAG	2 cut(s)	318, 1205
BfoI	RGCGCY	2 cut(s)	180, 409
BfuAI	ACCTGC	1 cut(s)	1165
BglI	GCCNNNNNGGC	2 cut(s)	615, 704
BisI	GCNGC	7 cut(s)	172, 175, 180, 394, 619, 641, 1118
BlpI	GCTNAGC	1 cut(s)	389
BlsI	GCNGC	7 cut(s)	173, 176, 181, 395, 620, 642, 1119
Bme1390I	CCNGG	5 cut(s)	411, 425, 431, 764, 986
Bme18I	GGWCC	4 cut(s)	427, 585, 601, 721
BmgBI	CACGTC	1 cut(s)	349
BmgT120I	GGNCC	6 cut(s)	427, 585, 601, 696, 697, 721
BmrFI	CCNGG	5 cut(s)	411, 425, 431, 764, 986
BmrI	ACTGGG	1 cut(s)	139
BmsI	GCATC	2 cut(s)	343, 704
BmuI	ACTGGG	1 cut(s)	139
BpiI	GAAGAC	1 cut(s)	766
BpmI	CTGGAG	2 cut(s)	547, 575
Bpu1102I	GCTNAGC	1 cut(s)	389
BpuMI	CCSGG	4 cut(s)	411, 425, 764, 986
BsaAI	YACGTR	1 cut(s)	805
BsaHI	GRCGYC	3 cut(s)	177, 406, 453
BsaJI	CCNNGG	3 cut(s)	172, 193, 409
BsaWI	WCCGGW	3 cut(s)	553, 661, 723
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	9 cut(s)	423, 430, 560, 582, 598, 692, 693, 769, 918
Bse118I	RCCGGY	1 cut(s)	236
Bse1I	ACTGG	2 cut(s)	134, 633
Bse21I	CCTNAGG	1 cut(s)	16
BseBI	CCWGG	1 cut(s)	431
BseDI	CCNNGG	3 cut(s)	172, 193, 409
BseGI	GGATG	2 cut(s)	719, 1168
BseLI	CCNNNNNNNGG	9 cut(s)	423, 430, 560, 582, 598, 692, 693, 769, 918
BseMII	CTCAG	2 cut(s)	30, 403
BseNI	ACTGG	2 cut(s)	134, 633
BseRI	GAGGAG	2 cut(s)	4, 1145
BseSI	GKGCMC	4 cut(s)	700, 909, 1032, 1142
BseX3I	CGGCCG	1 cut(s)	421
BseXI	GCAGC	2 cut(s)	405, 652
BseYI	CCCAGC	2 cut(s)	31, 644
BsgI	GTGCAG	2 cut(s)	659, 1071
Bsh1236I	CGCG	7 cut(s)	174, 182, 501, 607, 719, 883, 1094
Bsh1285I	CGRYCG	1 cut(s)	424
BshFI	GGCC	6 cut(s)	423, 434, 632, 698, 707, 731
BshNI	GGYRCC	2 cut(s)	176, 405
BsiEI	CGRYCG	1 cut(s)	424
BsiHKAI	GWGCWC	3 cut(s)	510, 1032, 1142
BsiSI	CCGG	9 cut(s)	237, 411, 424, 438, 554, 662, 724, 764, 985
BsiWI	CGTACG	2 cut(s)	801, 824
BslFI	GGGAC	3 cut(s)	598, 614, 881
BslI	CCNNNNNNNGG	9 cut(s)	423, 430, 560, 582, 598, 692, 693, 769, 918
BsmAI	GTCTC	1 cut(s)	146
BsmFI	GGGAC	3 cut(s)	598, 614, 881
BsmI	GAATGC	1 cut(s)	544
BsnI	GGCC	6 cut(s)	423, 434, 632, 698, 707, 731
Bsp120I	GGGCCC	1 cut(s)	696
Bsp1286I	GDGCHC	7 cut(s)	510, 700, 764, 909, 1020, 1032, 1142
Bsp143I	GATC	4 cut(s)	107, 413, 550, 682
Bsp1720I	GCTNAGC	1 cut(s)	389
BspANI	GGCC	6 cut(s)	423, 434, 632, 698, 707, 731
BspCNI	CTCAG	2 cut(s)	29, 402
BspFNI	CGCG	7 cut(s)	174, 182, 501, 607, 719, 883, 1094
BspMI	ACCTGC	1 cut(s)	1165
BspPI	GGATC	5 cut(s)	115, 408, 421, 545, 677
BspT107I	GGYRCC	2 cut(s)	176, 405
BsrFI	RCCGGY	1 cut(s)	236
BsrI	ACTGG	2 cut(s)	134, 633
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	3 cut(s)	172, 193, 409
BssMI	GATC	4 cut(s)	107, 413, 550, 682
BssNI	GRCGYC	3 cut(s)	177, 406, 453
BssSI	CACGAG	2 cut(s)	611, 1145
BssT1I	CCWWGG	1 cut(s)	193
Bst2BI	CACGAG	2 cut(s)	611, 1145
Bst2UI	CCWGG	1 cut(s)	431
Bst4CI	ACNGT	4 cut(s)	151, 226, 796, 829
BstACI	GRCGYC	3 cut(s)	177, 406, 453
BstBAI	YACGTR	1 cut(s)	805
BstDEI	CTNAG	3 cut(s)	16, 229, 389
BstDSI	CCRYGG	1 cut(s)	172
BstF5I	GGATG	2 cut(s)	719, 1168
BstFNI	CGCG	7 cut(s)	174, 182, 501, 607, 719, 883, 1094
BstH2I	RGCGCY	2 cut(s)	180, 409
BstHHI	GCGC	7 cut(s)	179, 408, 501, 519, 609, 719, 1094
BstKTI	GATC	4 cut(s)	110, 416, 553, 685
BstMAI	GTCTC	1 cut(s)	146
BstMBI	GATC	4 cut(s)	107, 413, 550, 682
BstMCI	CGRYCG	1 cut(s)	424
BstMWI	GCNNNNNNNGC	8 cut(s)	399, 505, 514, 615, 624, 704, 1074, 1141
BstNI	CCWGG	1 cut(s)	431
BstNSI	RCATGY	1 cut(s)	360
BstSCI	CCNGG	5 cut(s)	409, 423, 429, 762, 984
BstSLI	GKGCMC	4 cut(s)	700, 909, 1032, 1142
BstUI	CGCG	7 cut(s)	174, 182, 501, 607, 719, 883, 1094
BstV1I	GCAGC	2 cut(s)	405, 652
BstV2I	GAAGAC	1 cut(s)	766
BstX2I	RGATCY	1 cut(s)	413
BstYI	RGATCY	1 cut(s)	413
BstZI	CGGCCG	1 cut(s)	421
Bsu36I	CCTNAGG	1 cut(s)	16
BsuRI	GGCC	6 cut(s)	423, 434, 632, 698, 707, 731
BtgI	CCRYGG	1 cut(s)	172
BtrI	CACGTC	1 cut(s)	349
BtsCI	GGATG	2 cut(s)	719, 1168
BtsI	GCAGTG	2 cut(s)	162, 1142
BtsIMutI	CAGTG	3 cut(s)	162, 640, 1142
BveI	ACCTGC	1 cut(s)	1165
CaiI	CAGNNNCTG	1 cut(s)	525
CfoI	GCGC	7 cut(s)	179, 408, 501, 519, 609, 719, 1094
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	6 cut(s)	427, 585, 601, 696, 697, 721
Cfr42I	CCGCGG	1 cut(s)	175
CpoI	CGGWCCG	1 cut(s)	721
CseI	GACGC	4 cut(s)	171, 442, 555, 659
Csp6I	GTAC	4 cut(s)	802, 825, 898, 997
CspI	CGGWCCG	1 cut(s)	721
CviAII	CATG	1 cut(s)	357
CviQI	GTAC	4 cut(s)	802, 825, 898, 997
DdeI	CTNAG	3 cut(s)	16, 229, 389
DinI	GGCGCC	2 cut(s)	178, 407
DpnI	GATC	4 cut(s)	109, 415, 552, 684
DpnII	GATC	4 cut(s)	107, 413, 550, 682
DrdI	GACNNNNNNGTC	1 cut(s)	425
DseDI	GACNNNNNNGTC	1 cut(s)	425
EaeI	YGGCCR	3 cut(s)	421, 630, 705
EagI	CGGCCG	1 cut(s)	421
EciI	GGCGGA	2 cut(s)	255, 347
EclXI	CGGCCG	1 cut(s)	421
Eco130I	CCWWGG	1 cut(s)	193
Eco147I	AGGCCT	1 cut(s)	731
Eco24I	GRGCYC	3 cut(s)	700, 764, 1020
Eco47I	GGWCC	4 cut(s)	427, 585, 601, 721
Eco52I	CGGCCG	1 cut(s)	421
Eco81I	CCTNAGG	1 cut(s)	16
EcoO109I	RGGNCCY	5 cut(s)	427, 585, 601, 696, 697
EcoRII	CCWGG	1 cut(s)	429
EcoT14I	CCWWGG	1 cut(s)	193
EcoT22I	ATGCAT	1 cut(s)	358
EcoT38I	GRGCYC	3 cut(s)	700, 764, 1020
EgeI	GGCGCC	2 cut(s)	178, 407
EheI	GGCGCC	2 cut(s)	178, 407
ErhI	CCWWGG	1 cut(s)	193
FaeI	CATG	1 cut(s)	360
FaiI	YATR	4 cut(s)	294, 329, 358, 447
FaqI	GGGAC	3 cut(s)	598, 614, 881
FatI	CATG	1 cut(s)	356
FauI	CCCGC	7 cut(s)	388, 506, 612, 686, 876, 962, 1144
Fnu4HI	GCNGC	7 cut(s)	172, 175, 180, 394, 619, 641, 1118
FokI	GGATG	2 cut(s)	726, 1175
FriOI	GRGCYC	3 cut(s)	700, 764, 1020
Fsp4HI	GCNGC	7 cut(s)	172, 175, 180, 394, 619, 641, 1118
FspBI	CTAG	2 cut(s)	318, 1205
GlaI	GCGC	7 cut(s)	178, 407, 500, 518, 608, 718, 1093
GluI	GCNGC	7 cut(s)	172, 175, 180, 394, 619, 641, 1118
GsaI	CCCAGC	2 cut(s)	35, 648
GsuI	CTGGAG	2 cut(s)	547, 575
HaeII	RGCGCY	2 cut(s)	180, 409
HaeIII	GGCC	6 cut(s)	423, 434, 632, 698, 707, 731
HapII	CCGG	9 cut(s)	237, 411, 424, 438, 554, 662, 724, 764, 985
HgaI	GACGC	4 cut(s)	171, 442, 555, 659
HhaI	GCGC	7 cut(s)	179, 408, 501, 519, 609, 719, 1094
Hin1I	GRCGYC	3 cut(s)	177, 406, 453
Hin1II	CATG	1 cut(s)	360
Hin6I	GCGC	7 cut(s)	177, 406, 499, 517, 607, 717, 1092
HinP1I	GCGC	7 cut(s)	177, 406, 499, 517, 607, 717, 1092
HinfI	GANTC	4 cut(s)	21, 243, 878, 889
HpaII	CCGG	9 cut(s)	237, 411, 424, 438, 554, 662, 724, 764, 985
HphI	GGTGA	3 cut(s)	769, 1091, 1139
Hpy166II	GTNNAC	3 cut(s)	997, 1030, 1140
Hpy188III	TCNNGA	2 cut(s)	18, 526
Hpy8I	GTNNAC	3 cut(s)	997, 1030, 1140
Hpy99I	CGWCG	8 cut(s)	353, 422, 458, 506, 581, 584, 653, 782
HpyAV	CCTTC	5 cut(s)	92, 697, 710, 721, 1182
HpyCH4III	ACNGT	4 cut(s)	151, 226, 796, 829
HpyCH4IV	ACGT	4 cut(s)	348, 800, 804, 896
HpyCH4V	TGCA	7 cut(s)	356, 375, 640, 1030, 1052, 1140, 1174
HpyF10VI	GCNNNNNNNGC	8 cut(s)	399, 505, 514, 615, 624, 704, 1074, 1141
HpyF3I	CTNAG	3 cut(s)	16, 229, 389
HpySE526I	ACGT	4 cut(s)	348, 800, 804, 896
Hsp92I	GRCGYC	3 cut(s)	177, 406, 453
Hsp92II	CATG	1 cut(s)	360
HspAI	GCGC	7 cut(s)	177, 406, 499, 517, 607, 717, 1092
KasI	GGCGCC	2 cut(s)	176, 405
KflI	GGGWCCC	2 cut(s)	585, 601
KspI	CCGCGG	1 cut(s)	175
Kzo9I	GATC	4 cut(s)	107, 413, 550, 682
LmnI	GCTCC	5 cut(s)	528, 563, 921, 955, 1132
Lsp1109I	GCAGC	2 cut(s)	405, 652
LweI	GCATC	2 cut(s)	343, 704
MaeI	CTAG	2 cut(s)	318, 1205
MaeII	ACGT	4 cut(s)	348, 800, 804, 896
MaeIII	GTNAC	5 cut(s)	125, 263, 457, 796, 832
MalI	GATC	4 cut(s)	109, 415, 552, 684
MboI	GATC	4 cut(s)	107, 413, 550, 682
MboII	GAAGA	8 cut(s)	65, 68, 86, 89, 104, 107, 484, 766
MfeI	CAATTG	2 cut(s)	121, 622
MflI	RGATCY	1 cut(s)	413
MhlI	GDGCHC	7 cut(s)	510, 700, 764, 909, 1020, 1032, 1142
MlsI	TGGCCA	1 cut(s)	632
MluCI	AATT	4 cut(s)	45, 121, 289, 622
MluNI	TGGCCA	1 cut(s)	632
Mly113I	GGCGCC	2 cut(s)	177, 406
MmeI	TCCRAC	8 cut(s)	210, 441, 600, 691, 899, 954, 1140, 1189
Mox20I	TGGCCA	1 cut(s)	632
Mph1103I	ATGCAT	1 cut(s)	358
MroXI	GAANNNNTTC	1 cut(s)	8
MscI	TGGCCA	1 cut(s)	632
MseI	TTAA	1 cut(s)	816
MslI	CAYNNNNRTG	3 cut(s)	351, 1025, 1145
Msp20I	TGGCCA	1 cut(s)	632
MspA1I	CMGCKG	4 cut(s)	174, 402, 595, 839
MspI	CCGG	9 cut(s)	237, 411, 424, 438, 554, 662, 724, 764, 985
MspR9I	CCNGG	5 cut(s)	411, 425, 431, 764, 986
MunI	CAATTG	2 cut(s)	121, 622
Mva1269I	GAATGC	1 cut(s)	544
MvaI	CCWGG	1 cut(s)	431
MvnI	CGCG	7 cut(s)	174, 182, 501, 607, 719, 883, 1094
MwoI	GCNNNNNNNGC	8 cut(s)	399, 505, 514, 615, 624, 704, 1074, 1141
NarI	GGCGCC	2 cut(s)	177, 406
NciI	CCSGG	4 cut(s)	411, 425, 764, 986
NdeII	GATC	4 cut(s)	107, 413, 550, 682
NlaIII	CATG	1 cut(s)	360
NmuCI	GTSAC	3 cut(s)	125, 457, 796
NsiI	ATGCAT	1 cut(s)	358
NspI	RCATGY	1 cut(s)	360
OliI	CACNNNNGTG	1 cut(s)	1145
PaeI	GCATGC	1 cut(s)	360
PceI	AGGCCT	1 cut(s)	731
PcsI	WCGNNNNNNNCGW	2 cut(s)	357, 501
PctI	GAATGC	1 cut(s)	544
PdmI	GAANNNNTTC	1 cut(s)	8
PfeI	GAWTC	4 cut(s)	21, 243, 878, 889
Pfl23II	CGTACG	2 cut(s)	801, 824
PflFI	GACNNNGTC	1 cut(s)	347
PkrI	GCNGC	7 cut(s)	173, 176, 181, 395, 620, 642, 1119
PluTI	GGCGCC	2 cut(s)	180, 409
Ppu21I	YACGTR	1 cut(s)	805
PpuMI	RGGWCCY	3 cut(s)	427, 585, 601
Psp5II	RGGWCCY	3 cut(s)	427, 585, 601
Psp6I	CCWGG	1 cut(s)	429
PspFI	CCCAGC	2 cut(s)	31, 644
PspGI	CCWGG	1 cut(s)	429
PspLI	CGTACG	2 cut(s)	801, 824
PspOMI	GGGCCC	1 cut(s)	696
PspPI	GGNCC	6 cut(s)	427, 585, 601, 696, 697, 721
PspPPI	RGGWCCY	3 cut(s)	427, 585, 601
PstNI	CAGNNNCTG	1 cut(s)	525
PsuI	RGATCY	1 cut(s)	413
PsyI	GACNNNGTC	1 cut(s)	347
PvuII	CAGCTG	2 cut(s)	595, 839
RsaI	GTAC	4 cut(s)	803, 826, 899, 998
RsaNI	GTAC	4 cut(s)	802, 825, 898, 997
RseI	CAYNNNNRTG	3 cut(s)	351, 1025, 1145
Rsr2I	CGGWCCG	1 cut(s)	721
RsrII	CGGWCCG	1 cut(s)	721
SacII	CCGCGG	1 cut(s)	175
SaqAI	TTAA	1 cut(s)	816
SatI	GCNGC	7 cut(s)	172, 175, 180, 394, 619, 641, 1118
Sau3AI	GATC	4 cut(s)	107, 413, 550, 682
Sau96I	GGNCC	6 cut(s)	427, 585, 601, 696, 697, 721
ScrFI	CCNGG	5 cut(s)	411, 425, 431, 764, 986
SduI	GDGCHC	7 cut(s)	510, 700, 764, 909, 1020, 1032, 1142
SfaNI	GCATC	2 cut(s)	343, 704
SfiI	GGCCNNNNNGGCC	1 cut(s)	704
SfoI	GGCGCC	2 cut(s)	178, 407
Sfr303I	CCGCGG	1 cut(s)	175
SgrAI	CRCCGGYG	1 cut(s)	236
SgrBI	CCGCGG	1 cut(s)	175
SinI	GGWCC	4 cut(s)	427, 585, 601, 721
SmiMI	CAYNNNNRTG	3 cut(s)	351, 1025, 1145
SphI	GCATGC	1 cut(s)	360
Sse9I	AATT	4 cut(s)	45, 121, 289, 622
SseBI	AGGCCT	1 cut(s)	731
SspDI	GGCGCC	2 cut(s)	176, 405
SspMI	CTAG	2 cut(s)	318, 1205
StuI	AGGCCT	1 cut(s)	731
StyD4I	CCNGG	5 cut(s)	409, 423, 429, 762, 984
StyI	CCWWGG	1 cut(s)	193
TaaI	ACNGT	4 cut(s)	151, 226, 796, 829
TaiI	ACGT	4 cut(s)	351, 803, 807, 899
TaqI	TCGA	7 cut(s)	204, 210, 351, 474, 495, 655, 777
TaqII	GACCGA	1 cut(s)	760
TasI	AATT	4 cut(s)	45, 121, 289, 622
TauI	GCSGC	5 cut(s)	174, 177, 182, 621, 1120
TfiI	GAWTC	4 cut(s)	21, 243, 878, 889
Tru1I	TTAA	1 cut(s)	816
Tru9I	TTAA	1 cut(s)	816
TscAI	CASTG	3 cut(s)	169, 640, 1142
TseFI	GTSAC	3 cut(s)	125, 457, 796
TseI	GCWGC	2 cut(s)	393, 640
Tsp45I	GTSAC	3 cut(s)	125, 457, 796
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	4 cut(s)	476, 795, 860, 887
TspRI	CASTG	3 cut(s)	169, 640, 1142
Tth111I	GACNNNGTC	1 cut(s)	347
VneI	GTGCAC	2 cut(s)	1028, 1138
VpaK11BI	GGWCC	4 cut(s)	427, 585, 601, 721
XapI	RAATTY	1 cut(s)	45
XceI	RCATGY	1 cut(s)	360
XmnI	GAANNNNTTC	1 cut(s)	8
XspI	CTAG	2 cut(s)	318, 1205
Zsp2I	ATGCAT	1 cut(s)	358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.