MD01G1213900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012931
Physical Location & Seq
Reverse (-)
30744699 .. 30746600
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1213900.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGAGTACGATTACAGAACCCGAACCGGTCAAATGCTGACCCACCGACCCGCCACGTCATCAGCGCCTTCTAGCCATCCGATGTACGGACCTCCGTCGTCATCGTCGTCCATGTACCCGCGGGTGGGTCAGCAGAGCCACACGGCGGCCCCGCCCCCGTACGGCGGTAACGCAGGCCATCCGCAGCACCAGCCCCACCACCAAACCACAAATCCTCCTTCGTCTTCCTCCTCGGGATTGGGTATTCGAGTTACTATAAAACCGGAGTATCGGATCACTCCTCCGCCTCCGTTGTCATTGCACGTTGGAGATATACCGCGGAGCAATTTCCAGTTCGATTTTGATCTCGAGAGGAAAGTCTTGGCTGAATTAGAGAAGGAAACTCCAAATTGGGCCAAGCTCGGGCTGGAAAACATTCCACCACCGAGGGCTGTAGAACCACCACCTTCCGCGGGTTCTGGCGCAGATCCCGTTGTCAGCAAATATATCGCTTCAGGGCTCAGCCGGGAAGCAGTTCCTCTTGCAGTAGCAAATTACGGAGACAATCCTACTAAGGTTCGAGAATTCGTCAATGCATTCACCCTTCTACGAGAGATGGGATTTTCACCAAACGCTGTTGCTGAAGCCCTAATGATGTATGACAACGACACGGACAAGGCCCTAGCACATTTTCTCAACAGTTCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

24.81

Weight (kDa)

6.5

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBAP-1_UBA2 PF21267 196 - 221 6.9e-06 Ubiquitin-associated protein 1, UBA2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53330
fragaria_vesca FvH4_7g30890 FvH4_7g30890
malus_domestica MD01G1213900.v1.1 MD07G1283600.v1.1
prunus_persica Prupe.2G306000_v2.0.a1
pyrus_communis pycom01g22460 pycom07g25770
rosa_chinensis RchiOBHm_Chr1g0379961
rosa_laevigata RLG00000026345
rosa_multiflora Rmu_sc0004184.1_g000017
rosa_roxburghii Rroxscaffold_4G00279820
rosa_rugosa Rorug01G0415900
rosa_samantha Rh1AG440600 Rh1BG396300 Rh1CG409500 Rh1DG427100
rosa_wichuraiana Rw1G038300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 121, 319, 452
AclWI GGATC 2 cut(s) 281, 461
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 2 cut(s) 477, 642
AfaI GTAC 4 cut(s) 8, 86, 116, 161
AfiI CCNNNNNNNGG 8 cut(s) 86, 124, 125, 145, 161, 164, 402, 452
AgeI ACCGGT 1 cut(s) 26
AjiI CACGTC 1 cut(s) 57
AluBI AGCT 1 cut(s) 400
AluI AGCT 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 534
AlwI GGATC 2 cut(s) 281, 461
Ama87I CYCGRG 3 cut(s) 232, 347, 401
AoxI GGCC 4 cut(s) 147, 175, 393, 657
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 1 cut(s) 563
AsiGI ACCGGT 1 cut(s) 26
Asp700I GAANNNNTTC 2 cut(s) 414, 513
AspLEI GCGC 2 cut(s) 67, 464
AspS9I GGNCC 4 cut(s) 89, 148, 393, 658
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 2 cut(s) 571, 597
AvaI CYCGRG 3 cut(s) 232, 347, 401
AvaII GGWCC 1 cut(s) 89
BaeI ACNNNNGTAYC 2 cut(s) 251, 284
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 196
BccI CCATC 3 cut(s) 84, 186, 589
BceAI ACGGC 2 cut(s) 159, 178
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 534
BfaI CTAG 2 cut(s) 72, 662
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 2 cut(s) 147, 185
BlpI GCTNAGC 1 cut(s) 500
BlsI GCNGC 2 cut(s) 148, 186
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 3 cut(s) 232, 347, 401
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 4 cut(s) 89, 148, 393, 658
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 506
BoxI GACNNNNGTC 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 5 cut(s) 119, 231, 317, 425, 450
BsaWI WCCGGW 2 cut(s) 26, 262
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 8 cut(s) 86, 124, 125, 145, 161, 164, 402, 452
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 296
Bse8I GATNNNNATC 1 cut(s) 342
BseDI CCNNGG 5 cut(s) 119, 231, 317, 425, 450
BseGI GGATG 2 cut(s) 76, 178
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 8 cut(s) 86, 124, 125, 145, 161, 164, 402, 452
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 514
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 2 cut(s) 220, 270
BseXI GCAGC 1 cut(s) 196
Bsh1236I CGCG 3 cut(s) 121, 319, 452
BshFI GGCC 4 cut(s) 149, 177, 395, 659
BshTI ACCGGT 1 cut(s) 26
BsiHKCI CYCGRG 3 cut(s) 232, 347, 401
BsiSI CCGG 3 cut(s) 27, 263, 505
BsiWI CGTACG 1 cut(s) 159
BslI CCNNNNNNNGG 8 cut(s) 86, 124, 125, 145, 161, 164, 402, 452
BsmAI GTCTC 1 cut(s) 534
BsmI GAATGC 1 cut(s) 575
BsnI GGCC 4 cut(s) 149, 177, 395, 659
BsoBI CYCGRG 3 cut(s) 232, 347, 401
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 3 cut(s) 273, 343, 466
Bsp1720I GCTNAGC 1 cut(s) 500
BspANI GGCC 4 cut(s) 149, 177, 395, 659
BspCNI CTCAG 1 cut(s) 513
BspFNI CGCG 3 cut(s) 121, 319, 452
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 2 cut(s) 281, 461
BsrDI GCAATG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 5 cut(s) 119, 231, 317, 425, 450
BssMI GATC 3 cut(s) 273, 343, 466
Bst4CI ACNGT 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 2 cut(s) 500, 552
BstDSI CCRYGG 3 cut(s) 119, 317, 450
BstF5I GGATG 2 cut(s) 76, 178
BstFNI CGCG 3 cut(s) 121, 319, 452
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 2 cut(s) 67, 464
BstKTI GATC 3 cut(s) 276, 346, 469
BstMAI GTCTC 1 cut(s) 534
BstMBI GATC 3 cut(s) 273, 343, 466
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstPAI GACNNNNGTC 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 432
BstUI CGCG 3 cut(s) 121, 319, 452
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 4 cut(s) 149, 177, 395, 659
BtgI CCRYGG 3 cut(s) 119, 317, 450
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 2 cut(s) 76, 178
Cac8I GCNNGC 1 cut(s) 175
CfoI GCGC 2 cut(s) 67, 464
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 4 cut(s) 89, 148, 393, 658
Cfr42I CCGCGG 3 cut(s) 122, 320, 453
Csp6I GTAC 4 cut(s) 7, 85, 115, 160
CspAI ACCGGT 1 cut(s) 26
CviAII CATG 1 cut(s) 112
CviQI GTAC 4 cut(s) 7, 85, 115, 160
DdeI CTNAG 2 cut(s) 500, 552
DpnI GATC 3 cut(s) 275, 345, 468
DpnII GATC 3 cut(s) 273, 343, 466
EciI GGCGGA 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 477, 642
Eco88I CYCGRG 3 cut(s) 232, 347, 401
EcoO109I RGGNCCY 1 cut(s) 658
EcoRI GAATTC 1 cut(s) 563
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 501
FaeI CATG 1 cut(s) 115
FaiI YATR 5 cut(s) 113, 257, 314, 486, 639
FatI CATG 1 cut(s) 111
FauI CCCGC 5 cut(s) 58, 114, 126, 159, 445
Fnu4HI GCNGC 2 cut(s) 147, 185
FokI GGATG 2 cut(s) 63, 165
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 2 cut(s) 147, 185
FspBI CTAG 2 cut(s) 72, 662
GlaI GCGC 2 cut(s) 66, 463
GluI GCNGC 2 cut(s) 147, 185
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 4 cut(s) 149, 177, 395, 659
HapII CCGG 3 cut(s) 27, 263, 505
HhaI GCGC 2 cut(s) 67, 464
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 2 cut(s) 65, 462
HinP1I GCGC 2 cut(s) 65, 462
HpaII CCGG 3 cut(s) 27, 263, 505
HphI GGTGA 2 cut(s) 571, 597
Hpy188I TCNGA 2 cut(s) 81, 273
Hpy188III TCNNGA 5 cut(s) 234, 347, 349, 560, 687
Hpy99I CGWCG 2 cut(s) 100, 109
HpyAV CCTTC 5 cut(s) 78, 228, 370, 456, 593
HpyCH4III ACNGT 1 cut(s) 680
HpyCH4IV ACGT 2 cut(s) 56, 303
HpyCH4V TGCA 3 cut(s) 301, 524, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 500, 552
HpySE526I ACGT 2 cut(s) 56, 303
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 2 cut(s) 65, 462
KspI CCGCGG 3 cut(s) 122, 320, 453
Kzo9I GATC 3 cut(s) 273, 343, 466
LmnI GCTCC 1 cut(s) 321
LpnPI CCDG 9 cut(s) 40, 159, 203, 276, 344, 392, 444, 480, 518
Lsp1109I GCAGC 1 cut(s) 196
MaeI CTAG 2 cut(s) 72, 662
MaeII ACGT 2 cut(s) 56, 303
MaeIII GTNAC 2 cut(s) 167, 250
MalI GATC 3 cut(s) 275, 345, 468
MboI GATC 3 cut(s) 273, 343, 466
MboII GAAGA 1 cut(s) 216
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 5 cut(s) 325, 368, 388, 532, 563
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 9 cut(s) 102, 225, 238, 241, 291, 297, 345, 420, 528
Mph1103I ATGCAT 1 cut(s) 577
MroXI GAANNNNTTC 2 cut(s) 414, 513
MspA1I CMGCKG 3 cut(s) 121, 319, 452
MspI CCGG 3 cut(s) 27, 263, 505
MspR9I CCNGG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 575
MvnI CGCG 3 cut(s) 121, 319, 452
MwoI GCNNNNNNNGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 506
NdeII GATC 3 cut(s) 273, 343, 466
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 577
PaeR7I CTCGAG 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PctI GAATGC 1 cut(s) 575
PdmI GAANNNNTTC 2 cut(s) 414, 513
Pfl23II CGTACG 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 26
PkrI GCNGC 2 cut(s) 148, 186
PshAI GACNNNNGTC 1 cut(s) 94
PspLI CGTACG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 4 cut(s) 89, 148, 393, 658
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 4 cut(s) 8, 86, 116, 161
RsaNI GTAC 4 cut(s) 7, 85, 115, 160
SacII CCGCGG 3 cut(s) 122, 320, 453
SatI GCNGC 2 cut(s) 147, 185
Sau3AI GATC 3 cut(s) 273, 343, 466
Sau96I GGNCC 4 cut(s) 89, 148, 393, 658
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 1 cut(s) 501
SetI ASST 6 cut(s) 59, 94, 306, 402, 448, 558
SfcI CTRYAG 1 cut(s) 432
Sfr274I CTCGAG 1 cut(s) 347
Sfr303I CCGCGG 3 cut(s) 122, 320, 453
SgrBI CCGCGG 3 cut(s) 122, 320, 453
SinI GGWCC 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 5 cut(s) 325, 368, 388, 532, 563
SspMI CTAG 2 cut(s) 72, 662
StyD4I CCNGG 1 cut(s) 504
TaaI ACNGT 1 cut(s) 680
TaiI ACGT 2 cut(s) 59, 306
TaqI TCGA 4 cut(s) 247, 336, 348, 559
TasI AATT 5 cut(s) 325, 368, 388, 532, 563
TauI GCSGC 1 cut(s) 149
TseI GCWGC 1 cut(s) 184
TspDTI ATGAA 1 cut(s) 672
TspGWI ACGGA 5 cut(s) 84, 102, 279, 552, 665
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XhoI CTCGAG 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 414, 513
XspI CTAG 2 cut(s) 72, 662
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.