Rh1DG427100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012931
Physical Location & Seq
Reverse (-)
63988259 .. 63991843
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG427100.1

Sequence Viewer

Length: 693 bp
ATGGACTACGATTACCGCAAAACCTCATCCTACGGCGCGCAGATCCCGCCCTACCGCCCTGCCTCGTCATCACAGCCGATGTACGGACCGCCACAGCCGTCGTCATCGCTGTACCCCAGGGTCGGTCAACAACACGGTCAAGCGCCGGTTCCTACGCCGTCTCACGGCGGCGGAGGTCACCGTCCCGTCCCTCATCACCAGACCGCAACCACCTCAGCGCCTTCTTCCTCGGGGTTGGGCATTCGAGTTATGATAAAGCCAGAGTATCGGATCACTCCTCCGCCTCAATTGTTGACGCAAGTCGGAGACATACCCCGAAGCAATTTCCAATTTGACTTTGATTTCGAGAGGAAAGTTTTGGCTGAAGCGCAGAAGGAAACCCCGAATTGGGGCAAGCTGGGGTTGGAAAACCTTCCACCACCTAAGGCTGTGGAGCCGCCGTCTTCTGCTTCCTCCTCCTCGGGTTCTATTGGTAACCCCGTCATGAGGAAGTATGTCGCTTCAGGCCAAGATGTGGTACCTCGGGCAGTTGCAACTTATGGCGACAATCCTGAGAAGGTTCGGGAATTTGTCCATGGGTTCAACGCTTTACATGAGATGGGGTTTCCTTCAGACAGTGTTGCCGATGCCCTTCTCATGTATGATAATGACAAGGAGAAAGCACTTGCCTATCTAATCAACAGTTCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

24.9

Weight (kDa)

7.07

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53330
fragaria_vesca FvH4_7g30890 FvH4_7g30890
malus_domestica MD01G1213900.v1.1 MD07G1283600.v1.1
prunus_persica Prupe.2G306000_v2.0.a1
pyrus_communis pycom01g22460 pycom07g25770
rosa_chinensis RchiOBHm_Chr1g0379961
rosa_laevigata RLG00000026345
rosa_multiflora Rmu_sc0004184.1_g000017
rosa_roxburghii Rroxscaffold_4G00279820
rosa_rugosa Rorug01G0415900
rosa_samantha Rh1AG440600 Rh1BG396300 Rh1CG409500 Rh1DG427100
rosa_wichuraiana Rw1G038300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 517
AccB7I CCANNNNNTGG 1 cut(s) 514
AccII CGCG 1 cut(s) 38
AciI CCGC 9 cut(s) 16, 47, 55, 89, 168, 171, 204, 281, 437
AclWI GGATC 2 cut(s) 37, 278
AcsI RAATTY 1 cut(s) 566
AcuI CTGAAG 3 cut(s) 384, 486, 594
AfaI GTAC 3 cut(s) 83, 113, 519
AfiI CCNNNNNNNGG 8 cut(s) 83, 122, 164, 387, 388, 389, 486, 514
AgsI TTSAA 1 cut(s) 583
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 1 cut(s) 397
AluI AGCT 1 cut(s) 397
Alw26I GTCTC 2 cut(s) 165, 300
AlwI GGATC 2 cut(s) 37, 278
Ama87I CYCGRG 3 cut(s) 229, 460, 522
AoxI GGCC 1 cut(s) 505
ApoI RAATTY 1 cut(s) 566
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 411
Asp718I GGTACC 1 cut(s) 517
AspLEI GCGC 5 cut(s) 38, 40, 145, 220, 370
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 2 cut(s) 170, 188
AvaI CYCGRG 3 cut(s) 229, 460, 522
AvaII GGWCC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 423
BanI GGYRCC 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 435
BbvCI CCTCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 592
BceAI ACGGC 5 cut(s) 49, 82, 142, 181, 424
BciT130I CCWGG 1 cut(s) 118
BcoDI GTCTC 2 cut(s) 165, 300
BfoI RGCGCY 2 cut(s) 146, 221
BisI GCNGC 2 cut(s) 169, 437
BlsI GCNGC 2 cut(s) 170, 438
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 3 cut(s) 229, 460, 522
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 3 cut(s) 150, 435, 519
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 616
BoxI GACNNNNGTC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 214
BsaJI CCNNGG 6 cut(s) 116, 117, 228, 459, 521, 574
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 122, 164, 387, 388, 389, 486, 514
Bse118I RCCGGY 1 cut(s) 145
Bse21I CCTNAGG 1 cut(s) 423
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 6 cut(s) 116, 117, 228, 459, 521, 574
BseGI GGATG 1 cut(s) 26
BseLI CCNNNNNNNGG 8 cut(s) 83, 122, 164, 387, 388, 389, 486, 514
BseMII CTCAG 2 cut(s) 228, 543
BsePI GCGCGC 1 cut(s) 36
BseRI GAGGAG 3 cut(s) 267, 445, 448
BseYI CCCAGC 1 cut(s) 397
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 507
BshNI GGYRCC 1 cut(s) 517
BsiHKCI CYCGRG 3 cut(s) 229, 460, 522
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 2 cut(s) 168, 173
BslI CCNNNNNNNGG 8 cut(s) 83, 122, 164, 387, 388, 389, 486, 514
BsmAI GTCTC 2 cut(s) 165, 300
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 2 cut(s) 168, 173
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 1 cut(s) 507
BsoBI CYCGRG 3 cut(s) 229, 460, 522
Bsp143I GATC 2 cut(s) 42, 270
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 9 cut(s) 16, 47, 55, 89, 168, 171, 204, 281, 437
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 2 cut(s) 227, 544
BspFNI CGCG 1 cut(s) 38
BspHI TCATGA 2 cut(s) 483, 689
BspLI GGNNCC 3 cut(s) 150, 435, 519
BspPI GGATC 2 cut(s) 37, 278
BspT107I GGYRCC 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 6 cut(s) 116, 117, 228, 459, 521, 574
BssHII GCGCGC 1 cut(s) 36
BssMI GATC 2 cut(s) 42, 270
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 4 cut(s) 137, 182, 617, 683
BstC8I GCNNGC 2 cut(s) 38, 395
BstDEI CTNAG 3 cut(s) 214, 423, 552
BstDSI CCRYGG 1 cut(s) 574
BstEII GGTNACC 2 cut(s) 176, 473
BstF5I GGATG 1 cut(s) 26
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 2 cut(s) 146, 221
BstHHI GCGC 5 cut(s) 38, 40, 145, 220, 370
BstKTI GATC 2 cut(s) 45, 273
BstMAI GTCTC 2 cut(s) 165, 300
BstMBI GATC 2 cut(s) 42, 270
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 1 cut(s) 118
BstPAI GACNNNNGTC 1 cut(s) 299
BstPI GGTNACC 2 cut(s) 176, 473
BstSCI CCNGG 1 cut(s) 116
BstUI CGCG 1 cut(s) 38
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 1 cut(s) 507
BtgI CCRYGG 1 cut(s) 574
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 622
Cac8I GCNNGC 2 cut(s) 38, 395
CciI TCATGA 2 cut(s) 483, 689
CfoI GCGC 5 cut(s) 38, 40, 145, 220, 370
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 86
CpoI CGGWCCG 1 cut(s) 86
CseI GACGC 1 cut(s) 304
Csp6I GTAC 3 cut(s) 82, 112, 518
CspI CGGWCCG 1 cut(s) 86
CviAII CATG 5 cut(s) 484, 575, 593, 637, 690
CviJI RGCY 8 cut(s) 76, 97, 259, 362, 397, 428, 436, 507
CviKI_1 RGCY 8 cut(s) 76, 97, 259, 362, 397, 428, 436, 507
CviQI GTAC 3 cut(s) 82, 112, 518
DdeI CTNAG 3 cut(s) 214, 423, 552
DpnI GATC 2 cut(s) 44, 272
DpnII GATC 2 cut(s) 42, 270
EciI GGCGGA 2 cut(s) 186, 270
Eco130I CCWWGG 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 3 cut(s) 384, 486, 594
Eco81I CCTNAGG 1 cut(s) 423
Eco88I CYCGRG 3 cut(s) 229, 460, 522
Eco91I GGTNACC 2 cut(s) 176, 473
EcoO65I GGTNACC 2 cut(s) 176, 473
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 574
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 5 cut(s) 487, 578, 596, 640, 693
FaqI GGGAC 2 cut(s) 168, 173
FatI CATG 5 cut(s) 483, 574, 592, 636, 689
FauI CCCGC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 169, 437
FokI GGATG 1 cut(s) 13
Fsp4HI GCNGC 2 cut(s) 169, 437
GlaI GCGC 5 cut(s) 37, 39, 144, 219, 369
GluI GCNGC 2 cut(s) 169, 437
GsaI CCCAGC 1 cut(s) 401
HaeII RGCGCY 2 cut(s) 146, 221
HaeIII GGCC 1 cut(s) 507
HapII CCGG 1 cut(s) 146
HgaI GACGC 1 cut(s) 304
HhaI GCGC 5 cut(s) 38, 40, 145, 220, 370
Hin1II CATG 5 cut(s) 487, 578, 596, 640, 693
Hin6I GCGC 5 cut(s) 36, 38, 143, 218, 368
HinP1I GCGC 5 cut(s) 36, 38, 143, 218, 368
HincII GTYRAC 2 cut(s) 128, 294
HindII GTYRAC 2 cut(s) 128, 294
HpaII CCGG 1 cut(s) 146
HphI GGTGA 2 cut(s) 170, 188
Hpy166II GTNNAC 2 cut(s) 128, 294
Hpy188I TCNGA 3 cut(s) 270, 305, 613
Hpy188III TCNNGA 5 cut(s) 346, 484, 551, 563, 690
Hpy8I GTNNAC 2 cut(s) 128, 294
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 6 cut(s) 231, 367, 422, 550, 618, 641
HpyCH4III ACNGT 4 cut(s) 137, 182, 617, 683
HpyCH4V TGCA 1 cut(s) 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 3 cut(s) 214, 423, 552
Hsp92II CATG 5 cut(s) 487, 578, 596, 640, 693
HspAI GCGC 5 cut(s) 36, 38, 143, 218, 368
KpnI GGTACC 1 cut(s) 521
Kzo9I GATC 2 cut(s) 42, 270
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 9 cut(s) 72, 103, 130, 159, 212, 273, 383, 489, 564
LweI GCATC 1 cut(s) 616
MaeIII GTNAC 2 cut(s) 176, 473
MalI GATC 2 cut(s) 44, 272
MboI GATC 2 cut(s) 42, 270
MboII GAAGA 2 cut(s) 216, 435
MfeI CAATTG 1 cut(s) 287
MflI RGATCY 1 cut(s) 42
MluCI AATT 5 cut(s) 287, 322, 329, 385, 566
MmeI TCCRAC 2 cut(s) 283, 384
MroXI GAANNNNTTC 1 cut(s) 411
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 1 cut(s) 118
MunI CAATTG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 46
NcoI CCATGG 1 cut(s) 574
NdeII GATC 2 cut(s) 42, 270
NlaIII CATG 5 cut(s) 487, 578, 596, 640, 693
NlaIV GGNNCC 3 cut(s) 150, 435, 519
NmuCI GTSAC 1 cut(s) 176
PagI TCATGA 2 cut(s) 483, 689
PasI CCCWGGG 1 cut(s) 117
PauI GCGCGC 1 cut(s) 36
PctI GAATGC 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 411
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 2 cut(s) 170, 438
PshAI GACNNNNGTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 116
PspEI GGTNACC 2 cut(s) 176, 473
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 150, 435, 519
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 1 cut(s) 42
PteI GCGCGC 1 cut(s) 36
RsaI GTAC 3 cut(s) 83, 113, 519
RsaNI GTAC 3 cut(s) 82, 112, 518
Rsr2I CGGWCCG 1 cut(s) 86
RsrII CGGWCCG 1 cut(s) 86
SatI GCNGC 2 cut(s) 169, 437
Sau3AI GATC 2 cut(s) 42, 270
Sau96I GGNCC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 118
SetI ASST 8 cut(s) 26, 178, 215, 399, 414, 424, 523, 561
SfaNI GCATC 1 cut(s) 616
SinI GGWCC 1 cut(s) 86
Sse9I AATT 5 cut(s) 287, 322, 329, 385, 566
SsiI CCGC 9 cut(s) 16, 47, 55, 89, 168, 171, 204, 281, 437
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 4 cut(s) 137, 182, 617, 683
TaqI TCGA 2 cut(s) 244, 345
TaqII GACCGA 1 cut(s) 113
TasI AATT 5 cut(s) 287, 322, 329, 385, 566
TauI GCSGC 2 cut(s) 171, 439
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 1 cut(s) 622
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.