pycom01g22460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012931
Physical Location & Seq
Reverse (-)
20048893 .. 20049789
897 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22460.3

Sequence Viewer

Length: 423 bp
ATGTTTATTGAGAATCAACCTCCGTTGTCATCGCAGTTTGGAGATATACCGCGGAGCAATTTCCAGTTCGATTTTGATCTCGAGAGGAAAGTCTTGGCTGAATTAGAAAAGGAAACTCCGAATTGGGCCAAGCTCGGGCTGGAAAACATTCCACCACCGAGGGCTGTAGAACCACCACCTTCGTCGGGTTCTGGCGCAGATCCCGTTGTCAGCAAATATATCGCTTCAGGGCTCAACCGGGAAGCAGTTCCTCTTGCAGTTGCAAATTACGGAGACAATCCTACTAAGGTTCGAGAATTCGTCAATGCATTCACCCTTCTACGAGAGATGGGATTTTCACCAAACGCTGTTGCTGAAGCCCTAATGATGTATGACAACGACACGGACAAGGCCCTAGCACATTTTCTCAACAGTTCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.39

Weight (kDa)

4.64

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53330
fragaria_vesca FvH4_7g30890 FvH4_7g30890
malus_domestica MD01G1213900.v1.1 MD07G1283600.v1.1
prunus_persica Prupe.2G306000_v2.0.a1
pyrus_communis pycom01g22460 pycom07g25770
rosa_chinensis RchiOBHm_Chr1g0379961
rosa_laevigata RLG00000026345
rosa_multiflora Rmu_sc0004184.1_g000017
rosa_roxburghii Rroxscaffold_4G00279820
rosa_rugosa Rorug01G0415900
rosa_samantha Rh1AG440600 Rh1BG396300 Rh1CG409500 Rh1DG427100
rosa_wichuraiana Rw1G038300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 52
AciI CCGC 2 cut(s) 50, 52
AclWI GGATC 1 cut(s) 194
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 2 cut(s) 210, 375
AfiI CCNNNNNNNGG 2 cut(s) 135, 185
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 1 cut(s) 194
Ama87I CYCGRG 2 cut(s) 80, 134
AoxI GGCC 2 cut(s) 126, 390
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 2 cut(s) 147, 246
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 2 cut(s) 126, 391
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 304, 330
AvaI CYCGRG 2 cut(s) 80, 134
BanII GRGCYC 1 cut(s) 234
BccI CCATC 1 cut(s) 322
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 165
Bme1390I CCNGG 1 cut(s) 239
BmeT110I CYCGRG 2 cut(s) 80, 134
BmgT120I GGNCC 2 cut(s) 126, 391
BmrFI CCNGG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 50, 158
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 185
Bse1I ACTGG 1 cut(s) 64
Bse8I GATNNNNATC 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 50, 158
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 135, 185
BseNI ACTGG 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 128, 392
BsiHKCI CYCGRG 2 cut(s) 80, 134
BsiSI CCGG 1 cut(s) 238
BslI CCNNNNNNNGG 2 cut(s) 135, 185
BsmAI GTCTC 1 cut(s) 267
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 2 cut(s) 128, 392
BsoBI CYCGRG 2 cut(s) 80, 134
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 2 cut(s) 76, 199
BspACI CCGC 2 cut(s) 50, 52
BspANI GGCC 2 cut(s) 128, 392
BspFNI CGCG 1 cut(s) 52
BspPI GGATC 1 cut(s) 194
BsrI ACTGG 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 50, 158
BssMI GATC 2 cut(s) 76, 199
Bst4CI ACNGT 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 2 cut(s) 79, 202
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 2 cut(s) 76, 199
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 165
BstUI CGCG 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 2 cut(s) 128, 392
BtgI CCRYGG 1 cut(s) 50
BtgZI GCGATG 1 cut(s) 15
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 126, 391
Cfr42I CCGCGG 1 cut(s) 53
CviJI RGCY 8 cut(s) 98, 128, 133, 139, 164, 232, 359, 392
CviKI_1 RGCY 8 cut(s) 98, 128, 133, 139, 164, 232, 359, 392
DdeI CTNAG 1 cut(s) 285
DpnI GATC 2 cut(s) 78, 201
DpnII GATC 2 cut(s) 76, 199
Eco24I GRGCYC 1 cut(s) 234
Eco57I CTGAAG 2 cut(s) 210, 375
Eco88I CYCGRG 2 cut(s) 80, 134
EcoO109I RGGNCCY 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 234
FaiI YATR 3 cut(s) 47, 219, 372
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 1 cut(s) 395
GlaI GCGC 1 cut(s) 196
HaeIII GGCC 2 cut(s) 128, 392
HapII CCGG 1 cut(s) 238
HhaI GCGC 1 cut(s) 197
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HinfI GANTC 1 cut(s) 13
HpaII CCGG 1 cut(s) 238
HphI GGTGA 2 cut(s) 304, 330
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 4 cut(s) 80, 82, 293, 420
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 189, 326
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4V TGCA 3 cut(s) 257, 263, 308
HpyF3I CTNAG 1 cut(s) 285
HspAI GCGC 1 cut(s) 195
KspI CCGCGG 1 cut(s) 53
Kzo9I GATC 2 cut(s) 76, 199
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 5 cut(s) 77, 125, 177, 213, 251
MaeI CTAG 1 cut(s) 395
MalI GATC 2 cut(s) 78, 201
MboI GATC 2 cut(s) 76, 199
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 5 cut(s) 58, 101, 121, 265, 296
MnlI CCTC 4 cut(s) 30, 78, 153, 261
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 147, 246
MspA1I CMGCKG 1 cut(s) 52
MspI CCGG 1 cut(s) 238
MspR9I CCNGG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 308
MvnI CGCG 1 cut(s) 52
NciI CCSGG 1 cut(s) 239
NdeII GATC 2 cut(s) 76, 199
NsiI ATGCAT 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 80
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 2 cut(s) 147, 246
PfeI GAWTC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 126, 391
PsuI RGATCY 1 cut(s) 199
SacII CCGCGG 1 cut(s) 53
Sau3AI GATC 2 cut(s) 76, 199
Sau96I GGNCC 2 cut(s) 126, 391
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 234
SetI ASST 4 cut(s) 22, 135, 181, 291
SfcI CTRYAG 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 53
SgrBI CCGCGG 1 cut(s) 53
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 5 cut(s) 58, 101, 121, 265, 296
SsiI CCGC 2 cut(s) 50, 52
SspMI CTAG 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 413
TaqI TCGA 3 cut(s) 69, 81, 292
TasI AATT 5 cut(s) 58, 101, 121, 265, 296
TfiI GAWTC 1 cut(s) 13
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 3 cut(s) 12, 285, 398
XapI RAATTY 1 cut(s) 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 2 cut(s) 147, 246
XspI CTAG 1 cut(s) 395
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.