Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006456

Physical Location & Seq

Genomic Coordinates Reverse (-)

30935820 .. 30939537

Gene Structure 3718 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1216400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2397 bp

ATGGAGTGCAATAAAGATGATGCGGTCAAGGCTAAGGAACTTGCTGAGAAGAAGTTTGCAGAAAAAAATTATGCTAGTGCTAAGAAGTTTGTTTTGAAGGCTCTAAATTTGTATCCCGAGCTTGAGGGTCTCTCCCAAATGTTGACTATACTTGATGTTTACATCTCTTCGGAGAATAAAATTAATGGGGAAGCAGATTGGTATGGAGTACTTAGTGTTACTCCATATACTGATGATGAGACTATTAAGAAACAGTATAGAAAGTTGGCTTTCATGCTTCATCCTGATAAAAACAAGTCTTTTGGTGCAGAAGGCGCATTTAAGTTGGTTTCAGAGGCCTGGAGTTTGTTATCAGACAAGGCCAAGAGGGTAGCATGTAACCACAAGAGGAATGTACAAGGGTTTCAACAGGAAGCAGCGTCCCGTACTGGGGGTCCGTCCGTGCAACCTAGTGCAAGCACCTTTCAAAATTCTATGAATAGTGTCAGGAAGACTGTAAAAACGTCTCAACAGGAAGCTGCACACTGTAGTGGAGGTCCGTCAACGCAACCTAGTGCAAGTACTTTTCAGAATGTTATGAATGCGAAGACCCAGCAACCTAGTGCAACTACCTTTCAGAATGTTACGAATGCAAAGACTAAGCAACATAGTGCAACTACCTTTCAGAATGTTATGAATGCAAGGACCCAGCATGGTCCCACCCTGATAACTTCGTATGCAAAAGCTGCTACTTTTTGGACTGTTTGCAACCATTGCAAAACTCATTATCAGTATGTCAGGATGTATCTTCGTCATCTTCTTGTCTGCCCTAATTGTAGGAATACTTTCTTGGCTGTAGAACTGGCTCCACCTGCGGAGCTTTTCAAGGCGTCCAAGAGCTCTTCTTCCCGGCAGCATCAGAATTCAAGACAACATGCTGCTGCTGGTAACTCAAATAGTTCTGGAAGACACTGCGCTGCAACTCAAAATGTGGGAGCTGGGGGGTCTTTGGGTCCTAATTCAAACAACAACACAAACTTAAACCAGGGCTCTATCTCCAAAATGACTAGTATCAGTAGCACGGTTGCATCGGGTCATGTTGCTGTGGACGGGGGTCAGCAGACAAGTGAGAATGTAAAGAGGGATGCAGAGCGGAGACAGTCAATTGCTGAATGGGAAAGGAGTCAAAATTTGAACGGAAATCCTTCATTGAAAAGGAGAAGAATGGACGATCCTCATTTGAACGGCTATGATAAAAACAAGGCGAATCAAATGTCCATGGGAAATGGTGGAGCCGACTTGGGAGCTATATCTGAGTTGAGTAAAGGTAATGCTGGAACAAAAAGAATTTATGGTTTCTCTAGCACATACGATAGGCCTAAGGTCCTCAGAGAGGTTCCATATCCGGTAATAAAAAAAGCTTTGGTCGTGATGGCACTAACTTCAATTCGCAATTTTCTTGAGCGGAGCTCTGCAACTGAAGCAAAGCAGGCAAACAAAGAGCAAGAAACTGTGAAAGTAAATAAGAAACAGAAGAGCGTGGGAAATGTTGACACAAGTACAAACAGTGGTCCAAATGTAGAGGATAAGTCACCAATGCCGATCAACGTTCCAGATCCCGATTTTCATAATTTTGACTTGGATCGAACTGAAAGATCCTTTAGAGAGGACCAAGTTTGGGCTGCATATGATGATGATGGTATGCCTCGTTATTATGCTCGAATTCAGAAGGTGATCTCCTTGAGGCCGTTTAAGATGCGGATCAGTTGGCTTAACTCGAGAAGCAACAATGAATTGGGTCCGCTGAACTGGGTAGGTTCTGGTTTTGCTAAAACCTGTGGGGATTTCCGGCCTGGCAAATATGAAATTTCTGGTACTTTAAATTCTTTCTCAAGCAAGGTTAATTGGACAAAAGGCACACGAGGGGTGATTCGTGTATTTCCTACAAAGGGGGAAATCTGGGCTCTATATAGGAACTGGTCCCCTGATTGGAACCAGCATACCCCAGATGCAGTAATCAACAAATATGACATGGTGGAACTGCTCGATGACTTTACTGAGGAACACGGCGTGTCTGTCGCTCCTCTATCTAAGGTTGCTGGTTTTAAAACAGTGTTCCGTAAGCACACAGACCCCAAGGAAGTGAAGAGGATTCCTAAAGAGGAGATGTTCCGTTTCTCGCATCTGGTCCCTAGTCTCTTGCTTACTGGCGAAGAAGCTTATAATGCCCCAATGGGTTGTTGGGAATTGGATCCAGCAGCTCTCCCTTCGGAACTTTATCAAGTTATAACAGAAGTTCATGATGCACCAATGAAACAGAATGATGTTAAAACAGATGAAGAAATTCCAAAACCTCAACCAATGATGACCGACGACAGTGGTGCATGCCAAGTTGGCCAAGTTGGCCAAGGAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

DUF3444 DnaJ

Protein Analysis

799

Amino Acids

88.56

Weight (kDa)

9.19

Isoelectric Point (pI)

39.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 124	4.6e-18	DnaJ domain
Zn_ribbon_20	PF23551	245 - 279	7.6e-11	Zinc beta-ribbon domain
DUF3444	PF11926	526 - 732	4.5e-76	Domain of unknown function (DUF3444)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25560 AT5G53150 AT5G53150 AT5G53150 AT5G53150 AT5G53150 AT5G53150
fragaria_vesca	FvH4_7g31170
malus_domestica	MD01G1216400.v1.1 MD07G1286200.v1.1
prunus_persica	Prupe.2G308400_v2.0.a1 Prupe.2G308400_v2.0.a1 Prupe.2G308400_v2.0.a1
pyrus_communis	pycom01g22710 pycom07g25950
rosa_chinensis	RchiOBHm_Chr1g0380211
rosa_laevigata	RLG00000026319
rosa_multiflora	Rmu_sc0001232.1_g000026
rosa_roxburghii	Rroxscaffold_4G00279560
rosa_rugosa	Rorug01G0419000
rosa_samantha	Rh1AG443900 Rh1BG398900 Rh1CG412300 Rh1DG429500
rosa_wichuraiana	Rw1G038550 Rw1G038620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2202, 2267
AarI	CACCTGC	1 cut(s)	859
Acc36I	ACCTGC	1 cut(s)	859
AccBSI	CCGCTC	2 cut(s)	1132, 1444
AciI	CCGC	6 cut(s)	23, 854, 1132, 1444, 1738, 1781
AclI	AACGTT	1 cut(s)	1587
AclWI	GGATC	7 cut(s)	1205, 1589, 1629, 1629, 1748, 2225, 2238
AcoI	YGGCCR	2 cut(s)	2374, 2383
AcsI	RAATTY	9 cut(s)	106, 469, 901, 1168, 1326, 1701, 1845, 1861, 2322
AcuI	CTGAAG	1 cut(s)	1479
AcyI	GRCGYC	1 cut(s)	869
AdeI	CACNNNGTG	1 cut(s)	2050
AfaI	GTAC	6 cut(s)	210, 396, 427, 562, 1540, 1855
AfiI	CCNNNNNNNGG	6 cut(s)	429, 430, 1372, 1657, 1928, 1968
AhlI	ACTAGT	1 cut(s)	1046
AjnI	CCWGG	3 cut(s)	338, 1023, 1831
AjuI	GAANNNNNNNTTGG	2 cut(s)	812, 844
AleI	CACNNNNGTG	1 cut(s)	528
Alw21I	GWGCWC	2 cut(s)	881, 1451
Alw26I	GTCTC	5 cut(s)	134, 233, 510, 1129, 2180
AlwI	GGATC	7 cut(s)	1205, 1589, 1629, 1629, 1748, 2225, 2238
Ama87I	CYCGRG	2 cut(s)	116, 1756
AoxI	GGCC	7 cut(s)	336, 360, 1355, 1724, 1829, 2374, 2383
ApeKI	GCWGC	9 cut(s)	416, 518, 725, 892, 917, 920, 956, 1661, 2237
ApoI	RAATTY	9 cut(s)	106, 469, 901, 1168, 1326, 1701, 1845, 1861, 2322
ArsI	GACNNNNNNTTYG	2 cut(s)	1238, 1270
AseI	ATTAAT	1 cut(s)	183
Asp700I	GAANNNNTTC	3 cut(s)	824, 1183, 2322
AspLEI	GCGC	2 cut(s)	317, 956
AsuC2I	CCSGG	1 cut(s)	889
AsuHPI	GGTGA	3 cut(s)	1563, 1723, 1918
AvaI	CYCGRG	2 cut(s)	116, 1756
AxyI	CCTNAGG	1 cut(s)	1359
BalI	TGGCCA	2 cut(s)	2376, 2385
BamHI	GGATCC	1 cut(s)	2230
BanII	GRGCYC	4 cut(s)	881, 1031, 1451, 1945
BauI	CACGAG	1 cut(s)	1899
BbsI	GAAGAC	3 cut(s)	497, 593, 952
Bbv12I	GWGCWC	2 cut(s)	881, 1451
BbvI	GCAGC	9 cut(s)	428, 505, 712, 904, 904, 907, 943, 1648, 2249
BccI	CCATC	2 cut(s)	1405, 1670
BceAI	ACGGC	3 cut(s)	1240, 1711, 2062
BcgI	CGANNNNNNTGC	2 cut(s)	2342, 2376
BciT130I	CCWGG	3 cut(s)	340, 1025, 1833
BciVI	GTATCC	1 cut(s)	123
BcnI	CCSGG	1 cut(s)	889
BcoDI	GTCTC	5 cut(s)	134, 233, 510, 1129, 2180
BcuI	ACTAGT	1 cut(s)	1046
BfaI	CTAG	7 cut(s)	75, 450, 552, 600, 1047, 1341, 2172
BfmI	CTRYAG	2 cut(s)	526, 834
BfuAI	ACCTGC	1 cut(s)	859
BfuI	GTATCC	1 cut(s)	123
BglI	GCCNNNNNGGC	2 cut(s)	2373, 2382
BisI	GCNGC	9 cut(s)	417, 519, 726, 893, 918, 921, 957, 1662, 2238
BlsI	GCNGC	9 cut(s)	418, 520, 727, 894, 919, 922, 958, 1663, 2239
BmcAI	AGTACT	2 cut(s)	210, 562
Bme1390I	CCNGG	4 cut(s)	340, 889, 1025, 1833
BmeT110I	CYCGRG	2 cut(s)	116, 1756
BmrFI	CCNGG	4 cut(s)	340, 889, 1025, 1833
BmrI	ACTGGG	2 cut(s)	438, 1798
BmsI	GCATC	8 cut(s)	10, 904, 1076, 1114, 1725, 1978, 2170, 2272
BmuI	ACTGGG	2 cut(s)	438, 1798
BoxI	GACNNNNGTC	1 cut(s)	1092
BpiI	GAAGAC	3 cut(s)	497, 593, 952
BplI	GAGNNNNNCTC	4 cut(s)	116, 148, 1433, 1465
BpmI	CTGGAG	1 cut(s)	361
Bpu10I	CCTNAGC	1 cut(s)	33
BpuEI	CTTGAG	4 cut(s)	143, 1460, 1741, 1855
BpuMI	CCSGG	1 cut(s)	889
BsaBI	GATNNNNATC	1 cut(s)	1739
BsaHI	GRCGYC	1 cut(s)	869
BsaI	GGTCTC	1 cut(s)	134
BsaJI	CCNNGG	4 cut(s)	1024, 1257, 2115, 2386
BsaWI	WCCGGW	1 cut(s)	1384
Bsc4I	CCNNNNNNNGG	6 cut(s)	429, 430, 1372, 1657, 1928, 1968
Bse1I	ACTGG	5 cut(s)	433, 846, 1793, 1961, 2191
Bse21I	CCTNAGG	1 cut(s)	1359
Bse3DI	GCAATG	1 cut(s)	751
Bse8I	GATNNNNATC	1 cut(s)	1739
BseBI	CCWGG	3 cut(s)	340, 1025, 1833
BseDI	CCNNGG	4 cut(s)	1024, 1257, 2115, 2386
BseGI	GGATG	3 cut(s)	280, 786, 1129
BseJI	GATNNNNATC	1 cut(s)	1739
BseLI	CCNNNNNNNGG	6 cut(s)	429, 430, 1372, 1657, 1928, 1968
BseMI	GCAATG	1 cut(s)	751
BseMII	CTCAG	4 cut(s)	36, 1284, 1381, 2028
BseNI	ACTGG	5 cut(s)	433, 846, 1793, 1961, 2191
BseRI	GAGGAG	2 cut(s)	2052, 2156
BseXI	GCAGC	9 cut(s)	428, 505, 712, 904, 904, 907, 943, 1648, 2249
BseYI	CCCAGC	3 cut(s)	591, 687, 977
BsgI	GTGCAG	2 cut(s)	327, 504
BshFI	GGCC	7 cut(s)	338, 362, 1357, 1726, 1831, 2376, 2385
BsiHKAI	GWGCWC	2 cut(s)	881, 1451
BsiHKCI	CYCGRG	2 cut(s)	116, 1756
BsiSI	CCGG	3 cut(s)	889, 1385, 1828
BslFI	GGGAC	4 cut(s)	406, 681, 1945, 2153
BslI	CCNNNNNNNGG	6 cut(s)	429, 430, 1372, 1657, 1928, 1968
BsmAI	GTCTC	5 cut(s)	134, 233, 510, 1129, 2180
BsmBI	CGTCTC	1 cut(s)	510
BsmFI	GGGAC	4 cut(s)	406, 681, 1945, 2153
BsmI	GAATGC	3 cut(s)	586, 634, 682
BsnI	GGCC	7 cut(s)	338, 362, 1357, 1726, 1831, 2376, 2385
Bso31I	GGTCTC	1 cut(s)	134
BsoBI	CYCGRG	2 cut(s)	116, 1756
Bsp1286I	GDGCHC	4 cut(s)	881, 1031, 1451, 1945
Bsp1407I	TGTACA	1 cut(s)	394
Bsp143I	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
Bsp19I	CCATGG	1 cut(s)	1257
BspACI	CCGC	6 cut(s)	23, 854, 1132, 1444, 1738, 1781
BspANI	GGCC	7 cut(s)	338, 362, 1357, 1726, 1831, 2376, 2385
BspCNI	CTCAG	4 cut(s)	37, 1285, 1380, 2029
BspHI	TCATGA	1 cut(s)	2278
BspMI	ACCTGC	1 cut(s)	859
BspPI	GGATC	7 cut(s)	1205, 1589, 1629, 1629, 1748, 2225, 2238
BspQI	GCTCTTC	2 cut(s)	886, 1508
BspTNI	GGTCTC	1 cut(s)	134
BsrBI	CCGCTC	2 cut(s)	1132, 1444
BsrDI	GCAATG	1 cut(s)	751
BsrGI	TGTACA	1 cut(s)	394
BsrI	ACTGG	5 cut(s)	433, 846, 1793, 1961, 2191
BssECI	CCNNGG	4 cut(s)	1024, 1257, 2115, 2386
BssMI	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
BssNI	GRCGYC	1 cut(s)	869
BssSI	CACGAG	1 cut(s)	1899
BssT1I	CCWWGG	3 cut(s)	1257, 2115, 2386
Bst2BI	CACGAG	1 cut(s)	1899
Bst2UI	CCWGG	3 cut(s)	340, 1025, 1833
Bst6I	CTCTTC	4 cut(s)	172, 886, 1508, 2120
BstACI	GRCGYC	1 cut(s)	869
BstAPI	GCANNNNNTGC	2 cut(s)	725, 753
BstAUI	TGTACA	1 cut(s)	394
BstC8I	GCNNGC	3 cut(s)	457, 1470, 2365
BstDSI	CCRYGG	1 cut(s)	1257
BstENI	CCTNNNNNAGG	1 cut(s)	1370
BstF5I	GGATG	3 cut(s)	280, 786, 1129
BstHHI	GCGC	2 cut(s)	317, 956
BstKTI	GATC	8 cut(s)	1213, 1584, 1597, 1624, 1637, 1716, 1743, 2233
BstMAI	GTCTC	5 cut(s)	134, 233, 510, 1129, 2180
BstMBI	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
BstNI	CCWGG	3 cut(s)	340, 1025, 1833
BstNSI	RCATGY	3 cut(s)	378, 917, 2367
BstPAI	GACNNNNGTC	1 cut(s)	1092
BstSCI	CCNGG	4 cut(s)	338, 887, 1023, 1831
BstSFI	CTRYAG	2 cut(s)	526, 834
BstV1I	GCAGC	9 cut(s)	428, 505, 712, 904, 904, 907, 943, 1648, 2249
BstV2I	GAAGAC	3 cut(s)	497, 593, 952
BstX2I	RGATCY	3 cut(s)	1594, 1634, 2230
BstYI	RGATCY	3 cut(s)	1594, 1634, 2230
Bsu36I	CCTNAGG	1 cut(s)	1359
BsuI	GTATCC	1 cut(s)	123
BsuRI	GGCC	7 cut(s)	338, 362, 1357, 1726, 1831, 2376, 2385
BtgI	CCRYGG	1 cut(s)	1257
BtsCI	GGATG	3 cut(s)	280, 786, 1129
BtsI	GCAGTG	1 cut(s)	949
BtsIMutI	CAGTG	5 cut(s)	523, 949, 1552, 2097, 2362
BveI	ACCTGC	1 cut(s)	859
Cac8I	GCNNGC	3 cut(s)	457, 1470, 2365
CciI	TCATGA	1 cut(s)	2278
CfoI	GCGC	2 cut(s)	317, 956
CseI	GACGC	2 cut(s)	408, 858
Csp6I	GTAC	6 cut(s)	209, 395, 426, 561, 1539, 1854
CviAII	CATG	9 cut(s)	274, 375, 692, 914, 1076, 1258, 2011, 2279, 2364
CviQI	GTAC	6 cut(s)	209, 395, 426, 561, 1539, 1854
DpnI	GATC	8 cut(s)	1212, 1583, 1596, 1623, 1636, 1715, 1742, 2232
DpnII	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
DraI	TTTAAA	2 cut(s)	1860, 2086
DraIII	CACNNNGTG	1 cut(s)	2050
EaeI	YGGCCR	2 cut(s)	2374, 2383
Eam1104I	CTCTTC	4 cut(s)	172, 886, 1508, 2120
EarI	CTCTTC	4 cut(s)	172, 886, 1508, 2120
Ecl136II	GAGCTC	2 cut(s)	879, 1449
Eco130I	CCWWGG	3 cut(s)	1257, 2115, 2386
Eco147I	AGGCCT	2 cut(s)	338, 1357
Eco24I	GRGCYC	4 cut(s)	881, 1031, 1451, 1945
Eco31I	GGTCTC	1 cut(s)	134
Eco53kI	GAGCTC	2 cut(s)	879, 1449
Eco57I	CTGAAG	1 cut(s)	1479
Eco81I	CCTNAGG	1 cut(s)	1359
Eco88I	CYCGRG	2 cut(s)	116, 1756
EcoICRI	GAGCTC	2 cut(s)	879, 1449
EcoNI	CCTNNNNNAGG	1 cut(s)	1370
EcoO109I	RGGNCCY	3 cut(s)	684, 992, 1363
EcoRI	GAATTC	2 cut(s)	901, 1701
EcoRII	CCWGG	3 cut(s)	338, 1023, 1831
EcoT14I	CCWWGG	3 cut(s)	1257, 2115, 2386
EcoT38I	GRGCYC	4 cut(s)	881, 1031, 1451, 1945
ErhI	CCWWGG	3 cut(s)	1257, 2115, 2386
Esp3I	CGTCTC	1 cut(s)	510
FaeI	CATG	9 cut(s)	277, 378, 695, 917, 1079, 1261, 2014, 2282, 2367
FaqI	GGGAC	4 cut(s)	406, 681, 1945, 2153
FatI	CATG	9 cut(s)	273, 374, 691, 913, 1075, 1257, 2010, 2278, 2363
FauNDI	CATATG	1 cut(s)	1666
Fnu4HI	GCNGC	9 cut(s)	417, 519, 726, 893, 918, 921, 957, 1662, 2238
FokI	GGATG	3 cut(s)	267, 793, 1136
FriOI	GRGCYC	4 cut(s)	881, 1031, 1451, 1945
Fsp4HI	GCNGC	9 cut(s)	417, 519, 726, 893, 918, 921, 957, 1662, 2238
FspBI	CTAG	7 cut(s)	75, 450, 552, 600, 1047, 1341, 2172
GlaI	GCGC	2 cut(s)	316, 955
GluI	GCNGC	9 cut(s)	417, 519, 726, 893, 918, 921, 957, 1662, 2238
GsaI	CCCAGC	3 cut(s)	595, 691, 981
GsuI	CTGGAG	1 cut(s)	361
HaeIII	GGCC	7 cut(s)	338, 362, 1357, 1726, 1831, 2376, 2385
HapII	CCGG	3 cut(s)	889, 1385, 1828
HgaI	GACGC	2 cut(s)	408, 858
HhaI	GCGC	2 cut(s)	317, 956
Hin1I	GRCGYC	1 cut(s)	869
Hin1II	CATG	9 cut(s)	277, 378, 695, 917, 1079, 1261, 2014, 2282, 2367
Hin6I	GCGC	2 cut(s)	315, 954
HinP1I	GCGC	2 cut(s)	315, 954
HincII	GTYRAC	3 cut(s)	144, 543, 1531
HindII	GTYRAC	3 cut(s)	144, 543, 1531
HindIII	AAGCTT	2 cut(s)	1398, 2196
HinfI	GANTC	4 cut(s)	1162, 1246, 1909, 2131
HpaII	CCGG	3 cut(s)	889, 1385, 1828
HphI	GGTGA	3 cut(s)	1563, 1723, 1918
Hpy166II	GTNNAC	5 cut(s)	144, 160, 543, 1087, 1531
Hpy8I	GTNNAC	5 cut(s)	144, 160, 543, 1087, 1531
Hpy99I	CGWCG	1 cut(s)	2354
HpyAV	CCTTC	5 cut(s)	91, 305, 1194, 1702, 2256
HpyCH4IV	ACGT	2 cut(s)	503, 1587
HpySE526I	ACGT	2 cut(s)	503, 1587
Hsp92I	GRCGYC	1 cut(s)	869
Hsp92II	CATG	9 cut(s)	277, 378, 695, 917, 1079, 1261, 2014, 2282, 2367
HspAI	GCGC	2 cut(s)	315, 954
Kzo9I	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
LguI	GCTCTTC	2 cut(s)	886, 1508
LmnI	GCTCC	7 cut(s)	850, 856, 974, 1271, 1283, 1446, 2065
Lsp1109I	GCAGC	9 cut(s)	428, 505, 712, 904, 904, 907, 943, 1648, 2249
LweI	GCATC	8 cut(s)	10, 904, 1076, 1114, 1725, 1978, 2170, 2272
MaeI	CTAG	7 cut(s)	75, 450, 552, 600, 1047, 1341, 2172
MaeII	ACGT	2 cut(s)	503, 1587
MaeIII	GTNAC	5 cut(s)	217, 377, 622, 926, 1569
MalI	GATC	8 cut(s)	1212, 1583, 1596, 1623, 1636, 1715, 1742, 2232
MbiI	CCGCTC	2 cut(s)	1132, 1444
MboI	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
MfeI	CAATTG	1 cut(s)	1143
MflI	RGATCY	3 cut(s)	1594, 1634, 2230
MhlI	GDGCHC	4 cut(s)	881, 1031, 1451, 1945
MlsI	TGGCCA	2 cut(s)	2376, 2385
MluNI	TGGCCA	2 cut(s)	2376, 2385
MlyI	GAGTC	1 cut(s)	1171
Mox20I	TGGCCA	2 cut(s)	2376, 2385
MroXI	GAANNNNTTC	3 cut(s)	824, 1183, 2322
MscI	TGGCCA	2 cut(s)	2376, 2385
MslI	CAYNNNNRTG	1 cut(s)	528
Msp20I	TGGCCA	2 cut(s)	2376, 2385
MspA1I	CMGCKG	1 cut(s)	1783
MspI	CCGG	3 cut(s)	889, 1385, 1828
MspR9I	CCNGG	4 cut(s)	340, 889, 1025, 1833
MunI	CAATTG	1 cut(s)	1143
Mva1269I	GAATGC	3 cut(s)	586, 634, 682
MvaI	CCWGG	3 cut(s)	340, 1025, 1833
NciI	CCSGG	1 cut(s)	889
NcoI	CCATGG	1 cut(s)	1257
NdeI	CATATG	1 cut(s)	1666
NdeII	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
NlaIII	CATG	9 cut(s)	277, 378, 695, 917, 1079, 1261, 2014, 2282, 2367
NmuCI	GTSAC	1 cut(s)	1569
NspI	RCATGY	3 cut(s)	378, 917, 2367
OliI	CACNNNNGTG	1 cut(s)	528
PaeI	GCATGC	1 cut(s)	2367
PaeR7I	CTCGAG	1 cut(s)	1756
PagI	TCATGA	1 cut(s)	2278
PaqCI	CACCTGC	1 cut(s)	859
PceI	AGGCCT	2 cut(s)	338, 1357
PciSI	GCTCTTC	2 cut(s)	886, 1508
PctI	GAATGC	3 cut(s)	586, 634, 682
PdmI	GAANNNNTTC	3 cut(s)	824, 1183, 2322
PfeI	GAWTC	3 cut(s)	1246, 1909, 2131
PkrI	GCNGC	9 cut(s)	418, 520, 727, 894, 919, 922, 958, 1663, 2239
PleI	GAGTC	1 cut(s)	1170
PpsI	GAGTC	1 cut(s)	1170
PpuMI	RGGWCCY	3 cut(s)	684, 992, 1363
PshAI	GACNNNNGTC	1 cut(s)	1092
PshBI	ATTAAT	1 cut(s)	183
PsiI	TTATAA	2 cut(s)	2202, 2267
Psp124BI	GAGCTC	2 cut(s)	881, 1451
Psp1406I	AACGTT	1 cut(s)	1587
Psp5II	RGGWCCY	3 cut(s)	684, 992, 1363
Psp6I	CCWGG	3 cut(s)	338, 1023, 1831
PspFI	CCCAGC	3 cut(s)	591, 687, 977
PspGI	CCWGG	3 cut(s)	338, 1023, 1831
PspPPI	RGGWCCY	3 cut(s)	684, 992, 1363
PsuI	RGATCY	3 cut(s)	1594, 1634, 2230
RsaI	GTAC	6 cut(s)	210, 396, 427, 562, 1540, 1855
RsaNI	GTAC	6 cut(s)	209, 395, 426, 561, 1539, 1854
RseI	CAYNNNNRTG	1 cut(s)	528
SacI	GAGCTC	2 cut(s)	881, 1451
SapI	GCTCTTC	2 cut(s)	886, 1508
SatI	GCNGC	9 cut(s)	417, 519, 726, 893, 918, 921, 957, 1662, 2238
Sau3AI	GATC	8 cut(s)	1210, 1581, 1594, 1621, 1634, 1713, 1740, 2230
ScaI	AGTACT	2 cut(s)	210, 562
SchI	GAGTC	1 cut(s)	1171
ScrFI	CCNGG	4 cut(s)	340, 889, 1025, 1833
SduI	GDGCHC	4 cut(s)	881, 1031, 1451, 1945
SfaNI	GCATC	8 cut(s)	10, 904, 1076, 1114, 1725, 1978, 2170, 2272
SfcI	CTRYAG	2 cut(s)	526, 834
SfiI	GGCCNNNNNGGCC	1 cut(s)	2382
Sfr274I	CTCGAG	1 cut(s)	1756
SlaI	CTCGAG	1 cut(s)	1756
SmiMI	CAYNNNNRTG	1 cut(s)	528
SmlI	CTYRAG	5 cut(s)	122, 1439, 1720, 1756, 1870
SmoI	CTYRAG	5 cut(s)	122, 1439, 1720, 1756, 1870
SpeI	ACTAGT	1 cut(s)	1046
SphI	GCATGC	1 cut(s)	2367
SseBI	AGGCCT	2 cut(s)	338, 1357
SsiI	CCGC	6 cut(s)	23, 854, 1132, 1444, 1738, 1781
SspMI	CTAG	7 cut(s)	75, 450, 552, 600, 1047, 1341, 2172
SstI	GAGCTC	2 cut(s)	881, 1451
StuI	AGGCCT	2 cut(s)	338, 1357
StyD4I	CCNGG	4 cut(s)	338, 887, 1023, 1831
StyI	CCWWGG	3 cut(s)	1257, 2115, 2386
TaiI	ACGT	2 cut(s)	506, 1590
TaqI	TCGA	4 cut(s)	1624, 1699, 1757, 2025
TaqII	GACCGA	1 cut(s)	2363
TatI	WGTACW	4 cut(s)	208, 394, 560, 1538
TfiI	GAWTC	3 cut(s)	1246, 1909, 2131
TscAI	CASTG	5 cut(s)	530, 956, 1552, 2097, 2362
TseFI	GTSAC	1 cut(s)	1569
TseI	GCWGC	9 cut(s)	416, 518, 725, 892, 917, 920, 956, 1661, 2237
Tsp45I	GTSAC	1 cut(s)	1569
TspGWI	ACGGA	6 cut(s)	426, 430, 528, 1191, 2087, 2141
TspRI	CASTG	5 cut(s)	530, 956, 1552, 2097, 2362
VspI	ATTAAT	1 cut(s)	183
XagI	CCTNNNNNAGG	1 cut(s)	1370
XapI	RAATTY	9 cut(s)	106, 469, 901, 1168, 1326, 1701, 1845, 1861, 2322
XceI	RCATGY	3 cut(s)	378, 917, 2367
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2217
XhoI	CTCGAG	1 cut(s)	1756
XmnI	GAANNNNTTC	3 cut(s)	824, 1183, 2322
XspI	CTAG	7 cut(s)	75, 450, 552, 600, 1047, 1341, 2172
ZrmI	AGTACT	2 cut(s)	210, 562

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.