Rorug01G0419000

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006456
Physical Location & Seq
Reverse (-)
52099292 .. 52105554
6263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0419000.1

Sequence Viewer

Length: 4497 bp
ATGGGAGAGGCTGTTCTTGGGGCATTTCTCTCGGTGCTGCTTGAGAAGTTGGCGGATCGCGAGGTCATTGAGTACTTCGGAAGCCTTAAGGGGGTCGACAAAAAGGTGCTGGAGAAATGGAGAAGAACGTTGACTGCAATTGACGCGGTGCTGAGTGATGCGGAGGAGAAGCAACTAACACAAAGAGCAGTGAAACTATGGCTAGATGATCTCAGTGATTTGGCTTACGATGTTCAAGACATATTGGACATATTTGATACTAAAATGCTGAAGCGAAGGATAGAAAGACAACAAGGAAGCACAAGTAATTCATGGATCAGCTACCTCTCTAAACCCAAGTTCAATTTTAGTGTGAACTCAGAAATCAAGAAGATAAGTGATAAACTAGAAGAGATAACTACTCGAGAAAAGCAGTTTGGTCTGAAGAAACTTGGATTGTCTACCAAGCCATGGAAAATGCCACAAACTACTTCTCAACTAGATGGACCTGTGATAGGAAGGGATGAAGCAAAAGCACTAATTGTTGATAAATTGCTGTCAAAAGAAGAGCATAGTACCAGCAGTTTTCAGAATTTTCAAGTAGTTGCCATTGTTGGTACGCCTGGAATAGGAAAGACAACACTCGCAAAACATATATTCAACGATACTGCAACTGCACAGTTTTACCCAAAGGGATGGGTGTCTGTGTCCGATGACTTTGACCTTGTGAGGGTGACAACAGCAATTCTTGAATCGGTCACGTCAGCTCATGTTGAGGATTTCAAGCAGCTTAATTCAATTCAGGAGAAGTTAAGTAAGGAATTGGCTAGCAAGAAGTTTTTAATTGTTCTAGATGATGTCTGGCATACATGTCACTATGATCAATGGATAACACTGCAGTCCGCCTTTCGTGTTGGAGCAGCAGGAAGTAAGATCATTGTGACTACACGCGATGCAAATGTCGCAAACATGATGGGAGACACTAGCCCTTATAATTTGGGGTTAATATCAAAAGAGGATTGTTGGAAAATCTTTAAGCAACATGCACCCGTAAACGACAGACCCCAAAATGTTGAGTTACTCAAAGAGCAAATTGTTGTAAAATGCAATGGCTTACCGTTGGCTGCAAGAACTTTTGGTGGTCTTTTACGTTGTAAAAGAGTAGACGAATGGGAAGAAATATTAGACCACAAGATGTGGAATCTATCCGATCAGATGGCGGACATCCTTCCAGCATTGAAATTGAGCTATCATTATCTCCCTTCAAATTTAAAACGGTGTTTCACGTACTGCGCAATACTTCCAAATGATTATGAATTTGGAGAGATGCAATTGATCCTGTTGTGGATGGCAGAGGGTTTGATTCCGCAGCTACCAGAACAAAATAAACAAATAGAAGAGTTGGGCCGGCAGTATTTTCAAGAGTTGGTGTCTAGGTCATTATTTCAGAAGTCAAGCAAACGAAAGTCGCGATACATAATGCATGACCTCGTTACTGATTTGGCACGTTGGGCTGCAGGAAATTCATGTTCTAGATTGGAGGATATGCATAATTATGACTCGCAACTTAGATGTTTGCCCAAGGTTCGTCATTCGTCTTACATTCGTGGTCACCAAGATGGGGTTAAAAAGTTTGAGGTCTATTATGAGGCTATATGTTTGCGAACATTCTTACCACTTTCACTTTCAGATTTCCACGTTGATTATCTGGCTCTGAAGGTCACTTCTGATTTATTGCCCAAACTCCAATACTTACGGCTGCTCTCTTTGAATGGATATCAAATGACCGAGCTGCCAAATACAATTGGTAAATTGAAGCATCTACGGTATCTTGATCTTTCTCACACTCGAATAAGAAGTTTGCCGGACTCAACAGCCACTCTTTACAACTTGCAGACAGTGATATTACATGGTTGTTATCGGTTGAAGGCACTACCCACAAGCATGAGGAATCTAGTTAATTTGCGTCATCTCATCAATTCAGATACATTTTCATTGGAAGAAATGCCTCCTCAGCTTGGTCGATTGACTAATCTCCAAACAGTGTCTAATTTTGTTATTGGCAAAGGTAGTGGATTAAGAGTAAGAGAGATTGGGTCATTGTTGCATCTGCAAGGGAAATTGCACCTCTCAAGACTAGAGAATGTGGTTTTTGTCGAGGATGCTAGAAGTGCCAGCTTAAAGAGCAAGGAGAGGCTTGAAGCTCTGTTTCTTTCGTGGTCTTCTTCAAGTGACTCAACAGAAATTGCAGACCATGTGCTTGACATGTTACAACCTCATACCAAGCTCAAAGAGCTCACCATCGAGGGTTATGACGGATTGAAATTTTCAACATGGATTGGAGACCCTTCATTCTCTAATATGGTGCGTGTAAGGTTAGAACGTTGTCATCATTGTCAATTCTTGCCACCATTCGGACAATTGCCTTATCTCAAAGAACTTTATATACAAGAAATGGATGCAGTGGAAAGCGTTGGTCTTGAGTTTTATGGAGAAGGTAACTTGCCGTTTCAAGCTTTAGAGACCCTTAAGTTTCAGAATCTGAAATATTGGAAGAAGTGGTCTCCTTGCCAACAAAATGAGGGGGTTGGGGTTTTCTCTTGCCTAGAAAAGCTTTCCATCAAGGGCTGCCCCAAATTGGAGGGTAGTTTACCGAAGAAGCTTGATTCATTAGCACAGCTGGAGATATGTGGATGTGAGGAACTGGTGATTTTAATATCCAATTATAAACAGCTTCATGAATCAAACATAAAAAATTGCAAGATGGTGGTTTGCGACACAAGTCCAGCTCTCTTAGAGAGGTTGACATTTTCAAATATTTCAGAGTTGAGATTCCAAACAGAGGCGTTCATGAAAAGCTTGAAAAATGTTAAAGAGTTGAATATTACTGGTTGTGAGGAGCTGACATGTTCCTTTCAGCATGAGGATAGATTACTGCAACACTTGATTTCTCTTGGTAGTTTGTATATTGAAGACAACTCTGCACTTGTTGAAAAGCTAGGGAAAGAAGCAGAGCAGTTGGTGCAATTGCAAATACTGGATTGCAAGCTTAAACGTCTGGAATTGAGCAAGTGTGGAACCTTTTTGAAGGTGCCAGAAGGATTGCATCACCTTACATCTCTTCAAGAGCTTCACATAAAGGGATGTTCAAGTCTTGTTTCTTTTCCGGATGTTGGTCTGCCACCTTGTCTTAAAGTTATAGAAATTCAGAACTGCGATTCTCTTTTGCACTTTGCAAAATACCAGATTGCTCCAAGCCTAAGAAGAATAGGGATTCGGGAATGTGAGAAATTAAAATCATTGATAGAGAAAAAGGAGGTGGTGGTGGACAGCTCTTGTTCGTCTTATTGTCTCGAGTATTTGCAGATAAGGAACTGTCCATCTCTGTTATCCTTATTATGCAAACCCCAATTATCCAGGGCCCTTAAACAGCTTCGCATATGGGATTGTGAACAGCTGGAGTTAATAACCGAGAGGTTCCTCGATGACACCTGTCAACTCGAAGAAATTCATATCACAACATGCCCAAACCTTAAATCCTTACCTGAGGGACTGTGCCACCTCACCAATCTTCAGTCATTATATATTTGGGAATGTCAAAGTCTGGTTTCGTTGCCGAGATTGAGTGTGTGGCCAAGAGTGATGAATATCGAAGCTTGCGAGAAATTGGGGGTGGCACACTTGCTGAGAGACATGATGCACTCTAATAATCTTGAGGTTTTGACGATTGATTACTGTGAAGGTTTGACTACTCCTTCCTTTCCCCCCAACCTAACATCACTTGAAATTTACAAGATCAAGAATTGTAAGGCGCTGATGGAATCTCAAGGGTTGCACATACTCACATCTTTGAGAGAATTGACTCTATGGGGTAAAGATGATCCAGGTCTGGTGTGCTTTCCACCTGGTGAGAATTCGAAGGAGAAGGAGATGCTGCTCCCCAGATCTCTTGTTACATTGATAATTCAAGGCTTCCAAAATCTAAAGAAACTGAGCAAGGACTTTCAATTCCTCACCTCTCTTGAAACTCTTGAAATCTGGAACTGTCCGAAGCTTACAACCATTCCAGAGAAGGGCCTGCCTCTTTCACTTGCGCAACTTGAGATAATTGAGTCTGCTTATGCTCAAAACTGTAGAGCACATCCTTTTCCTCCTCCACAGAATATGATATTTGGATTCTCAATTCCAGCCTTTCCAGTTGATTTACTTGCCGAGGGGACTGATTTTAATTGTATTGGTGATTGGGTTGTGGAGGCCACCGTAAAGAAGAAGCTTAATGTTATGAGTGTTCATCTGACGAGTTCAAAGTCTTTGGTTGGAGTAGCTGGAGTTGCATTACAGATTCAAAAGAAGAAGAAGGGACGGGACGTGGATGTTATGGTGAGCTTCTTGGTGGGAATTCTAGAAGTTTACTTAGTACTGAAAATTATGACACACCTGGAGTTCATCTCCACCATAGGCAAGCAGCAGTGGTTCATTCAGAGACCTACCTTCCCTTGCTCCTCAAAGTCGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

1498

Amino Acids

170.26

Weight (kDa)

7.18

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 99 5.9e-20 Rx N-terminal domain
NB-ARC PF00931 190 - 342 1.1e-33 NB-ARC domain
WHD_DRP PF23559 426 - 495 1.3e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 572 - 839 2.6e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 812 4.1e-36 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 972, 2706
Acc16I TGCGCA 2 cut(s) 1273, 4074
AccB1I GGYRCC 1 cut(s) 3070
AccB7I CCANNNNNTGG 1 cut(s) 450
AccI GTMKAC 3 cut(s) 96, 440, 1143
AccII CGCG 4 cut(s) 60, 146, 930, 1450
AccIII TCCGGA 1 cut(s) 3145
AciI CCGC 6 cut(s) 53, 146, 161, 882, 1199, 1346
AclI AACGTT 2 cut(s) 128, 2362
AclWI GGATC 4 cut(s) 63, 323, 1309, 3854
AcoI YGGCCR 1 cut(s) 3611
AcuI CTGAAG 4 cut(s) 290, 443, 1715, 3536
AdeI CACNNNGTG 1 cut(s) 3887
AfaI GTAC 5 cut(s) 74, 556, 598, 1268, 4398
AflII CTTAAG 2 cut(s) 86, 2507
AflIII ACRYGT 3 cut(s) 848, 2244, 2885
AjiI CACGTC 2 cut(s) 741, 4348
AjnI CCWGG 5 cut(s) 601, 3395, 3862, 3883, 4416
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AloI GAACNNNNNNTCC 1 cut(s) 29
Alw21I GWGCWC 2 cut(s) 2277, 4120
Alw26I GTCTC 7 cut(s) 951, 2316, 2495, 2547, 3335, 3663, 4456
AlwI GGATC 4 cut(s) 63, 323, 1309, 3854
AlwNI CAGNNNCTG 1 cut(s) 2521
Ama87I CYCGRG 2 cut(s) 402, 3332
Aor13HI TCCGGA 1 cut(s) 3145
AoxI GGCC 5 cut(s) 1384, 3399, 3611, 4054, 4233
ApaI GGGCCC 1 cut(s) 3403
Asp700I GAANNNNTTC 1 cut(s) 3486
AspLEI GCGC 3 cut(s) 1274, 3793, 4075
AspS9I GGNCC 5 cut(s) 485, 1384, 3399, 3400, 4054
AsuII TTCGAA 1 cut(s) 3896
AsuNHI GCTAGC 1 cut(s) 806
AvaI CYCGRG 2 cut(s) 402, 3332
AvaII GGWCC 1 cut(s) 485
AxyI CCTNAGG 1 cut(s) 3525
BaeGI GKGCMC 1 cut(s) 3403
BalI TGGCCA 1 cut(s) 3613
BanI GGYRCC 1 cut(s) 3070
BanII GRGCYC 2 cut(s) 2277, 3403
BbsI GAAGAC 2 cut(s) 2193, 2958
Bbv12I GWGCWC 2 cut(s) 2277, 4120
BbvCI CCTCAGC 1 cut(s) 1992
BceAI ACGGC 2 cut(s) 1751, 2470
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 5 cut(s) 603, 3397, 3864, 3885, 4418
BclI TGATCA 1 cut(s) 859
BcoDI GTCTC 7 cut(s) 951, 2316, 2495, 2547, 3335, 3663, 4456
BfmI CTRYAG 3 cut(s) 875, 1494, 4111
BfoI RGCGCY 1 cut(s) 3794
BfrI CTTAAG 2 cut(s) 86, 2507
BglII AGATCT 1 cut(s) 3923
BmcAI AGTACT 2 cut(s) 74, 4398
Bme1390I CCNGG 5 cut(s) 603, 3397, 3864, 3885, 4418
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 2 cut(s) 402, 3332
BmgBI CACGTC 2 cut(s) 741, 4348
BmgT120I GGNCC 5 cut(s) 485, 1384, 3399, 3400, 4054
BmiI GGNNCC 4 cut(s) 3058, 3072, 3401, 3458
BmrFI CCNGG 5 cut(s) 603, 3397, 3864, 3885, 4418
BmtI GCTAGC 1 cut(s) 810
BoxI GACNNNNGTC 2 cut(s) 2760, 3471
BpiI GAAGAC 2 cut(s) 2193, 2958
BplI GAGNNNNNCTC 2 cut(s) 4412, 4444
BpmI CTGGAG 5 cut(s) 131, 2681, 3458, 4326, 4439
Bpu10I CCTNAGC 1 cut(s) 1992
Bpu14I TTCGAA 1 cut(s) 3896
BpuEI CTTGAG 6 cut(s) 62, 2095, 2480, 3713, 3789, 4100
BsaAI YACGTR 1 cut(s) 1266
BsaBI GATNNNNATC 1 cut(s) 3626
BsaI GGTCTC 4 cut(s) 2316, 2495, 2547, 4456
BsaJI CCNNGG 4 cut(s) 449, 1560, 3396, 4191
BsaWI WCCGGW 1 cut(s) 3145
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bse118I RCCGGY 1 cut(s) 1386
Bse1I ACTGG 4 cut(s) 2688, 2872, 3021, 4175
Bse21I CCTNAGG 1 cut(s) 3525
Bse3DI GCAATG 1 cut(s) 1093
Bse8I GATNNNNATC 1 cut(s) 3626
BseAI TCCGGA 1 cut(s) 3145
BseBI CCWGG 5 cut(s) 603, 3397, 3864, 3885, 4418
BseDI CCNNGG 4 cut(s) 449, 1560, 3396, 4191
BseJI GATNNNNATC 1 cut(s) 3626
BseMI GCAATG 1 cut(s) 1093
BseMII CTCAG 7 cut(s) 143, 226, 372, 2006, 3516, 3656, 3962
BseNI ACTGG 4 cut(s) 2688, 2872, 3021, 4175
BseRI GAGGAG 5 cut(s) 179, 1980, 2891, 4122, 4471
BseSI GKGCMC 1 cut(s) 3403
BsgI GTGCAG 2 cut(s) 639, 2946
Bsh1236I CGCG 4 cut(s) 60, 146, 930, 1450
BshFI GGCC 5 cut(s) 1386, 3401, 3613, 4056, 4235
BshNI GGYRCC 1 cut(s) 3070
BsiHKAI GWGCWC 2 cut(s) 2277, 4120
BsiHKCI CYCGRG 2 cut(s) 402, 3332
BsiSI CCGG 3 cut(s) 1387, 1844, 3146
BslFI GGGAC 4 cut(s) 3543, 4210, 4353, 4358
BsmAI GTCTC 7 cut(s) 951, 2316, 2495, 2547, 3335, 3663, 4456
BsmFI GGGAC 4 cut(s) 3543, 4210, 4353, 4358
BsnI GGCC 5 cut(s) 1386, 3401, 3613, 4056, 4235
Bso31I GGTCTC 4 cut(s) 2316, 2495, 2547, 4456
BsoBI CYCGRG 2 cut(s) 402, 3332
Bsp119I TTCGAA 1 cut(s) 3896
Bsp120I GGGCCC 1 cut(s) 3399
Bsp1286I GDGCHC 3 cut(s) 2277, 3403, 4120
Bsp13I TCCGGA 1 cut(s) 3145
Bsp19I CCATGG 1 cut(s) 449
Bsp68I TCGCGA 2 cut(s) 60, 1450
BspACI CCGC 6 cut(s) 53, 146, 161, 882, 1199, 1346
BspANI GGCC 5 cut(s) 1386, 3401, 3613, 4056, 4235
BspCNI CTCAG 7 cut(s) 144, 225, 371, 2005, 3517, 3657, 3963
BspEI TCCGGA 1 cut(s) 3145
BspFNI CGCG 4 cut(s) 60, 146, 930, 1450
BspHI TCATGA 2 cut(s) 2716, 2829
BspLI GGNNCC 4 cut(s) 3058, 3072, 3401, 3458
BspMAI CTGCAG 2 cut(s) 879, 1498
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 4 cut(s) 63, 323, 1309, 3854
BspQI GCTCTTC 1 cut(s) 540
BspT104I TTCGAA 1 cut(s) 3896
BspT107I GGYRCC 1 cut(s) 3070
BspTI CTTAAG 2 cut(s) 86, 2507
BspTNI GGTCTC 4 cut(s) 2316, 2495, 2547, 4456
BsrDI GCAATG 1 cut(s) 1093
BsrFI RCCGGY 1 cut(s) 1386
BsrI ACTGG 4 cut(s) 2688, 2872, 3021, 4175
BssAI RCCGGY 1 cut(s) 1386
BssECI CCNNGG 4 cut(s) 449, 1560, 3396, 4191
BssT1I CCWWGG 2 cut(s) 449, 1560
Bst2UI CCWGG 5 cut(s) 603, 3397, 3864, 3885, 4418
Bst6I CTCTTC 4 cut(s) 384, 540, 1371, 3105
BstAFI CTTAAG 2 cut(s) 86, 2507
BstAPI GCANNNNNTGC 1 cut(s) 3001
BstBAI YACGTR 1 cut(s) 1266
BstBI TTCGAA 1 cut(s) 3896
BstC8I GCNNGC 7 cut(s) 808, 1388, 2155, 3026, 3637, 4058, 4442
BstDSI CCRYGG 1 cut(s) 449
BstEII GGTNACC 1 cut(s) 1589
BstENI CCTNNNNNAGG 2 cut(s) 492, 606
BstFNI CGCG 4 cut(s) 60, 146, 930, 1450
BstH2I RGCGCY 1 cut(s) 3794
BstHHI GCGC 3 cut(s) 1274, 3793, 4075
BstMAI GTCTC 7 cut(s) 951, 2316, 2495, 2547, 3335, 3663, 4456
BstMWI GCNNNNNNNGC 9 cut(s) 143, 941, 1490, 1993, 2150, 2163, 2272, 3001, 4310
BstNI CCWGG 5 cut(s) 603, 3397, 3864, 3885, 4418
BstNSI RCATGY 5 cut(s) 852, 1025, 2248, 2889, 3504
BstPAI GACNNNNGTC 2 cut(s) 2760, 3471
BstPI GGTNACC 1 cut(s) 1589
BstSCI CCNGG 5 cut(s) 601, 3395, 3862, 3883, 4416
BstSFI CTRYAG 3 cut(s) 875, 1494, 4111
BstSLI GKGCMC 1 cut(s) 3403
BstUI CGCG 4 cut(s) 60, 146, 930, 1450
BstV2I GAAGAC 2 cut(s) 2193, 2958
BstX2I RGATCY 1 cut(s) 3923
BstXI CCANNNNNNTGG 1 cut(s) 675
BstYI RGATCY 1 cut(s) 3923
Bsu36I CCTNAGG 1 cut(s) 3525
BsuRI GGCC 5 cut(s) 1386, 3401, 3613, 4056, 4235
BtgI CCRYGG 1 cut(s) 449
BtgZI GCGATG 1 cut(s) 945
BtrI CACGTC 2 cut(s) 741, 4348
BtsI GCAGTG 4 cut(s) 195, 872, 2448, 4454
BtsIMutI CAGTG 7 cut(s) 195, 220, 872, 1884, 2028, 2448, 4454
BtuMI TCGCGA 2 cut(s) 60, 1450
Cac8I GCNNGC 7 cut(s) 808, 1388, 2155, 3026, 3637, 4058, 4442
CaiI CAGNNNCTG 1 cut(s) 2521
CciI TCATGA 2 cut(s) 2716, 2829
CfoI GCGC 3 cut(s) 1274, 3793, 4075
Cfr10I RCCGGY 1 cut(s) 1386
Cfr13I GGNCC 5 cut(s) 485, 1384, 3399, 3400, 4054
CseI GACGC 2 cut(s) 152, 1934
CsiI ACCWGGT 1 cut(s) 3883
Csp6I GTAC 5 cut(s) 73, 555, 597, 1267, 4397
CspCI CAANNNNNGTGG 2 cut(s) 2032, 2067
CviQI GTAC 5 cut(s) 73, 555, 597, 1267, 4397
DraI TTTAAA 1 cut(s) 1251
DraIII CACNNNGTG 1 cut(s) 3887
EaeI YGGCCR 1 cut(s) 3611
Eam1104I CTCTTC 4 cut(s) 384, 540, 1371, 3105
EarI CTCTTC 4 cut(s) 384, 540, 1371, 3105
EciI GGCGGA 3 cut(s) 68, 871, 1214
Ecl136II GAGCTC 1 cut(s) 2275
Eco130I CCWWGG 2 cut(s) 449, 1560
Eco24I GRGCYC 2 cut(s) 2277, 3403
Eco31I GGTCTC 4 cut(s) 2316, 2495, 2547, 4456
Eco32I GATATC 1 cut(s) 1757
Eco47I GGWCC 1 cut(s) 485
Eco53kI GAGCTC 1 cut(s) 2275
Eco57I CTGAAG 4 cut(s) 290, 443, 1715, 3536
Eco81I CCTNAGG 1 cut(s) 3525
Eco88I CYCGRG 2 cut(s) 402, 3332
Eco91I GGTNACC 1 cut(s) 1589
EcoICRI GAGCTC 1 cut(s) 2275
EcoNI CCTNNNNNAGG 2 cut(s) 492, 606
EcoO109I RGGNCCY 3 cut(s) 3399, 3400, 4054
EcoO65I GGTNACC 1 cut(s) 1589
EcoRI GAATTC 2 cut(s) 3892, 4377
EcoRII CCWGG 5 cut(s) 601, 3395, 3862, 3883, 4416
EcoRV GATATC 1 cut(s) 1757
EcoT14I CCWWGG 2 cut(s) 449, 1560
EcoT22I ATGCAT 2 cut(s) 1464, 1530
EcoT38I GRGCYC 2 cut(s) 2277, 3403
ErhI CCWWGG 2 cut(s) 449, 1560
FaqI GGGAC 4 cut(s) 3543, 4210, 4353, 4358
FauNDI CATATG 1 cut(s) 3419
FbaI TGATCA 1 cut(s) 859
FblI GTMKAC 3 cut(s) 96, 440, 1143
FriOI GRGCYC 2 cut(s) 2277, 3403
FspI TGCGCA 2 cut(s) 1273, 4074
GlaI GCGC 3 cut(s) 1273, 3792, 4074
GsuI CTGGAG 5 cut(s) 131, 2681, 3458, 4326, 4439
HaeII RGCGCY 1 cut(s) 3794
HaeIII GGCC 5 cut(s) 1386, 3401, 3613, 4056, 4235
HapII CCGG 3 cut(s) 1387, 1844, 3146
HgaI GACGC 2 cut(s) 152, 1934
HhaI GCGC 3 cut(s) 1274, 3793, 4075
Hin6I GCGC 3 cut(s) 1272, 3791, 4073
HinP1I GCGC 3 cut(s) 1272, 3791, 4073
HincII GTYRAC 4 cut(s) 97, 132, 2784, 3476
HindII GTYRAC 4 cut(s) 97, 132, 2784, 3476
HindIII AAGCTT 8 cut(s) 2493, 2591, 2639, 2836, 3026, 3633, 4031, 4250
HpaII CCGG 3 cut(s) 1387, 1844, 3146
HpyCH4IV ACGT 9 cut(s) 128, 740, 1129, 1265, 1486, 1677, 2362, 3034, 4347
HpyF10VI GCNNNNNNNGC 9 cut(s) 143, 941, 1490, 1993, 2150, 2163, 2272, 3001, 4310
HpySE526I ACGT 9 cut(s) 128, 740, 1129, 1265, 1486, 1677, 2362, 3034, 4347
HspAI GCGC 3 cut(s) 1272, 3791, 4073
Kpn2I TCCGGA 1 cut(s) 3145
KroI GCCGGC 1 cut(s) 1386
KroNI GCCGGC 1 cut(s) 1388
Ksp22I TGATCA 1 cut(s) 859
LguI GCTCTTC 1 cut(s) 540
LmnI GCTCC 5 cut(s) 896, 2878, 3235, 3921, 4484
MabI ACCWGGT 1 cut(s) 3883
MaeII ACGT 9 cut(s) 128, 740, 1129, 1265, 1486, 1677, 2362, 3034, 4347
MfeI CAATTG 5 cut(s) 138, 1310, 1782, 2399, 3005
MflI RGATCY 1 cut(s) 3923
MhlI GDGCHC 3 cut(s) 2277, 3403, 4120
MlsI TGGCCA 1 cut(s) 3613
MluNI TGGCCA 1 cut(s) 3613
MlyI GAGTC 5 cut(s) 1532, 1841, 2207, 3835, 4100
MmeI TCCRAC 3 cut(s) 874, 983, 4276
Mox20I TGGCCA 1 cut(s) 3613
Mph1103I ATGCAT 2 cut(s) 1464, 1530
MroI TCCGGA 1 cut(s) 3145
MroNI GCCGGC 1 cut(s) 1386
MroXI GAANNNNTTC 1 cut(s) 3486
MscI TGGCCA 1 cut(s) 3613
MslI CAYNNNNRTG 3 cut(s) 1533, 1596, 1922
Msp20I TGGCCA 1 cut(s) 3613
MspA1I CMGCKG 2 cut(s) 2659, 3436
MspCI CTTAAG 2 cut(s) 86, 2507
MspI CCGG 3 cut(s) 1387, 1844, 3146
MspR9I CCNGG 5 cut(s) 603, 3397, 3864, 3885, 4418
MunI CAATTG 5 cut(s) 138, 1310, 1782, 2399, 3005
MvaI CCWGG 5 cut(s) 603, 3397, 3864, 3885, 4418
MvnI CGCG 4 cut(s) 60, 146, 930, 1450
MwoI GCNNNNNNNGC 9 cut(s) 143, 941, 1490, 1993, 2150, 2163, 2272, 3001, 4310
NaeI GCCGGC 1 cut(s) 1388
NcoI CCATGG 1 cut(s) 449
NdeI CATATG 1 cut(s) 3419
NgoMIV GCCGGC 1 cut(s) 1386
NheI GCTAGC 1 cut(s) 806
NlaIV GGNNCC 4 cut(s) 3058, 3072, 3401, 3458
NmeAIII GCCGAG 2 cut(s) 3621, 4216
NmuCI GTSAC 7 cut(s) 712, 736, 851, 919, 1589, 1699, 2210
NruI TCGCGA 2 cut(s) 60, 1450
NsbI TGCGCA 2 cut(s) 1273, 4074
NsiI ATGCAT 2 cut(s) 1464, 1530
NspI RCATGY 5 cut(s) 852, 1025, 2248, 2889, 3504
NspV TTCGAA 1 cut(s) 3896
PaeR7I CTCGAG 2 cut(s) 402, 3332
PagI TCATGA 2 cut(s) 2716, 2829
PciI ACATGT 3 cut(s) 848, 2244, 2885
PciSI GCTCTTC 1 cut(s) 540
PcsI WCGNNNNNNNCGW 2 cut(s) 1447, 3636
PdiI GCCGGC 1 cut(s) 1388
PdmI GAANNNNTTC 1 cut(s) 3486
PflMI CCANNNNNTGG 1 cut(s) 450
PleI GAGTC 5 cut(s) 1532, 1841, 2207, 3835, 4099
PpsI GAGTC 5 cut(s) 1532, 1841, 2207, 3835, 4099
Ppu21I YACGTR 1 cut(s) 1266
PscI ACATGT 3 cut(s) 848, 2244, 2885
PshAI GACNNNNGTC 2 cut(s) 2760, 3471
PsiI TTATAA 2 cut(s) 972, 2706
Psp124BI GAGCTC 1 cut(s) 2277
Psp1406I AACGTT 2 cut(s) 128, 2362
Psp6I CCWGG 5 cut(s) 601, 3395, 3862, 3883, 4416
PspEI GGTNACC 1 cut(s) 1589
PspGI CCWGG 5 cut(s) 601, 3395, 3862, 3883, 4416
PspN4I GGNNCC 4 cut(s) 3058, 3072, 3401, 3458
PspOMI GGGCCC 1 cut(s) 3399
PspPI GGNCC 5 cut(s) 485, 1384, 3399, 3400, 4054
PsrI GAACNNNNNNTAC 2 cut(s) 2409, 2441
PstI CTGCAG 2 cut(s) 879, 1498
PstNI CAGNNNCTG 1 cut(s) 2521
PsuI RGATCY 1 cut(s) 3923
PvuII CAGCTG 2 cut(s) 2659, 3436
RruI TCGCGA 2 cut(s) 60, 1450
RsaI GTAC 5 cut(s) 74, 556, 598, 1268, 4398
RsaNI GTAC 5 cut(s) 73, 555, 597, 1267, 4397
RseI CAYNNNNRTG 3 cut(s) 1533, 1596, 1922
SacI GAGCTC 1 cut(s) 2277
SalI GTCGAC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 540
Sau96I GGNCC 5 cut(s) 485, 1384, 3399, 3400, 4054
ScaI AGTACT 2 cut(s) 74, 4398
SchI GAGTC 5 cut(s) 1532, 1841, 2207, 3835, 4100
ScrFI CCNGG 5 cut(s) 603, 3397, 3864, 3885, 4418
SduI GDGCHC 3 cut(s) 2277, 3403, 4120
SexAI ACCWGGT 1 cut(s) 3883
SfcI CTRYAG 3 cut(s) 875, 1494, 4111
Sfr274I CTCGAG 2 cut(s) 402, 3332
SfuI TTCGAA 1 cut(s) 3896
SinI GGWCC 1 cut(s) 485
SlaI CTCGAG 2 cut(s) 402, 3332
SmiMI CAYNNNNRTG 3 cut(s) 1533, 1596, 1922
SsiI CCGC 6 cut(s) 53, 146, 161, 882, 1199, 1346
SspI AATATT 4 cut(s) 1161, 2528, 2797, 2863
SstI GAGCTC 1 cut(s) 2277
StyD4I CCNGG 5 cut(s) 601, 3395, 3862, 3883, 4416
StyI CCWWGG 2 cut(s) 449, 1560
TaiI ACGT 9 cut(s) 131, 743, 1132, 1268, 1489, 1680, 2365, 3037, 4350
TaqII GACCGA 2 cut(s) 724, 1781
TatI WGTACW 2 cut(s) 72, 4396
TscAI CASTG 7 cut(s) 195, 220, 879, 1884, 2028, 2448, 4454
TseFI GTSAC 7 cut(s) 712, 736, 851, 919, 1589, 1699, 2210
Tsp45I GTSAC 7 cut(s) 712, 736, 851, 919, 1589, 1699, 2210
TspGWI ACGGA 1 cut(s) 2310
TspRI CASTG 7 cut(s) 195, 220, 879, 1884, 2028, 2448, 4454
Van91I CCANNNNNTGG 1 cut(s) 450
Vha464I CTTAAG 2 cut(s) 86, 2507
VpaK11BI GGWCC 1 cut(s) 485
XagI CCTNNNNNAGG 2 cut(s) 492, 606
XbaI TCTAGA 3 cut(s) 829, 1511, 4381
XceI RCATGY 5 cut(s) 852, 1025, 2248, 2889, 3504
XhoI CTCGAG 2 cut(s) 402, 3332
XmiI GTMKAC 3 cut(s) 96, 440, 1143
XmnI GAANNNNTTC 1 cut(s) 3486
ZrmI AGTACT 2 cut(s) 74, 4398
Zsp2I ATGCAT 2 cut(s) 1464, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.