Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0006456

Physical Location & Seq

Genomic Coordinates Reverse (-)

34827753 .. 34831557

Gene Structure 3805 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1286200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGGAGTGCAATAAAGATGATGCGGTCAAGGCTAAGGAACTTGCTGAGAGGAAGTTTGCAGAAAGAAATTATGCTAGTGCTAAGAAGTTTGTTTTGAAAGCTCTAAATTTGTGTCCCGAGCTTGAGGGTCTCTCCCAAATGTTGACTATACTTGATGTTTACATCTCTTCGGAGAATAAAATTAATGGGGAAGCAGATTGGTATGGAGTACTTAGTGTTACTCCGTATGCTGATGATGAGACAATTAAGAAAGAGTATAGAAAGTTGGCTTTCATGCTTCATCCTGATAAAAACAAGTGTTTTGGTGCAGAAGGCGCATTTAAGTTGGTTTCAGAGGCCTGGACTTTGTTATCAGACAAGGCCAAGAGGGTAGCATATAACCACATGAGGAATGTACAAGGGTTTCAACGGGAAGCAGTGTCCCGTACTGGGGGTCCGTCAGTGCAACCTAGTGCAAGCACCTTTCAAAATTGTATGAATAGTGTCAGGAAGACTGTAAAAGCGTCTCAACAGGAAGCTGCACACCGTAGTGGAGGTCCGTCAACGCAACCTAGTGCAAGTACCTTTCAGAATGTTATGAATGCGAGGACCCAGCGCGGTTCCACCCATATACCTTCATATGCAAAAGCTGCTACATTTTGGACCATTTGCAACCATTGCAAAACTCATTATCAGTATGTCAGAATGTATCTACATCATCTTCTTGTCTGTCCTAATTGTAAGAAGACTTTCTTGGCTGTAGAACAGGCTCCACCTGCAGAGATTTTCAAGTCGTCCAAGAGCTCTTCTTCCCAGCAGCATCAGAATTCAAGACACTATGCTGCTGGTGGTAACTCAAATAGTTCTGGAAGAAACTGTGCTGCAGCTCAAAATGTAGGAGCTGGGGGGTCTTCAGGTTCTAATTCAAACAACAACACAAACTTGAAGCAGGGCTCTGCCACCAAAGTGACAAGTATCGGTAGCACAGTTGCATCGGGTCACGCTGCTGTGGATGCGGTTCAGCAGGCAAGTGAGAATATGAATAGAAATTCAGAGCGGAGACAGTCAATTGCTGAATGGGAAAGGAGTCAAAATTTGAAGGGAAATCCTACATTGAAAAAGAGAAGAACGGATGATCCTCATTTGAACGGTTATGATAAAAAAAGGCCTAATCAAATGGCCATGGGAAGTGGTGGAGCCGACTTGGGAGGTATATCTGAGTTGAGTAAAGGTAATTCTGGAACGAAAAGAATTTATGATTTCTCTAGTGCACAGGACAAACCTAAGGTCCTCAGAGAGGTTCCGTATCCGGTAATAAGAAAAGTTCTAATTGAGACGGCACTAACTTCAATTCGCAATTTTCTTGCGCGGAGCTCAGCAACTGAAGCAAAGCAGGCAAAGAAAGAGCAAGAAACTGTGAAAGTAAAAAAGAAACAGAAGAGTGTGGTAAATGTTGACACAAGCACAAACAGTGCTCCAGATGTAGAGGATAAGTCACCAATGCCGATCAACGTTCCAGACCCCGATTTTCATAATTTTGACTTGGATCGAACTGAAAGATCCTTTGGAGAGGACCAAGTTTGGGCTGCATATGATAATGATGGTATGCCTCGTTATTATGGTCGAATTCAAAAGGTGATCTCCTTGAAGCCGTTTAAGATGCGGGTCAGTTGGCTTAACTCGAGAAGCAACAATGAATTGGGTACGCTGAACTGGGTAGGTTCTGGTTTTGCTAAAACTTGTGGTGATTTCAGGCCTGGCAAATATGCAATATCTGATACTTTAAATTCTTTCTCAAGCAAGGTTAATTGGACAAAAGGCACACGAGGGATGATTCGCATATTTCCTACAAGGGGTGAAATCTGGGCTCTATATAGGAACTGGTCCCCTGAATGGAATCAGCATACCCCAGATGCAGTAATCAACAAATATGACATGGTGGAACTGCTTGACGAGTTTACTGAGGAACATGGTGTGTCTGTCGCTCCTCTATCTAAGGTCGCTGGTTTTAAAACAGTGTTTCGTAAGCACACAGACCCCAAGGAAGTGCAACGGATTCCTAAAGAGGAGATGTTCCGTTTCTCGCATCGGGTCCCTAGTCACTTACTTACAGGTGAAGAAGCTCATAATGCTCCAAAGGGTTGTTGGGAATTGGATCCAGCAGCTCTCCCTTCGGAGCTTTATCAAGTTATGACAGAAGCTCGTGATGCACCAGTGAAACAGAACGATGGTAAAACAAATGAAGAGATTCCGAAACCTCTACCAATGATGACCGACGACAATGGTACATGCCAAGTCGGCCAAGGAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

DUF3444 DnaJ

Protein Analysis

764

Amino Acids

84.85

Weight (kDa)

9.27

Isoelectric Point (pI)

37.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 127	2.1e-19	DnaJ domain
Zn_ribbon_20	PF23551	213 - 247	1.4e-11	Zinc beta-ribbon domain
DUF3444	PF11926	494 - 700	2.2e-76	Domain of unknown function (DUF3444)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25560 AT5G53150 AT5G53150 AT5G53150 AT5G53150 AT5G53150 AT5G53150
fragaria_vesca	FvH4_7g31170
malus_domestica	MD01G1216400.v1.1 MD07G1286200.v1.1
prunus_persica	Prupe.2G308400_v2.0.a1 Prupe.2G308400_v2.0.a1 Prupe.2G308400_v2.0.a1
pyrus_communis	pycom01g22710 pycom07g25950
rosa_chinensis	RchiOBHm_Chr1g0380211
rosa_laevigata	RLG00000026319
rosa_multiflora	Rmu_sc0001232.1_g000026
rosa_roxburghii	Rroxscaffold_4G00279560
rosa_rugosa	Rorug01G0419000
rosa_samantha	Rh1AG443900 Rh1BG398900 Rh1CG412300 Rh1DG429500
rosa_wichuraiana	Rw1G038550 Rw1G038620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	763
Acc36I	ACCTGC	1 cut(s)	763
AccBSI	CCGCTC	1 cut(s)	1036
AccII	CGCG	2 cut(s)	597, 1348
AciI	CCGC	6 cut(s)	23, 597, 995, 1036, 1348, 1642
AclI	AACGTT	1 cut(s)	1491
AclWI	GGATC	5 cut(s)	1109, 1533, 1533, 2129, 2142
AcoI	YGGCCR	2 cut(s)	1158, 2278
AcsI	RAATTY	7 cut(s)	106, 805, 1027, 1072, 1230, 1605, 1765
AcuI	CTGAAG	2 cut(s)	876, 1383
AfaI	GTAC	6 cut(s)	210, 396, 427, 562, 1684, 2266
AfiI	CCNNNNNNNGG	6 cut(s)	429, 430, 1276, 1561, 1832, 1872
AjnI	CCWGG	2 cut(s)	338, 1735
AjuI	GAANNNNNNNTTGG	4 cut(s)	716, 748, 1527, 1559
AleI	CACNNNNGTG	2 cut(s)	528, 944
AloI	GAACNNNNNNTCC	2 cut(s)	1099, 1131
Alw21I	GWGCWC	4 cut(s)	785, 1252, 1355, 1456
Alw26I	GTCTC	5 cut(s)	134, 233, 510, 1033, 1307
Alw44I	GTGCAC	1 cut(s)	1248
AlwI	GGATC	5 cut(s)	1109, 1533, 1533, 2129, 2142
AlwNI	CAGNNNCTG	1 cut(s)	1361
Ama87I	CYCGRG	2 cut(s)	116, 1660
AoxI	GGCC	6 cut(s)	336, 360, 1145, 1158, 1733, 2278
ApaLI	GTGCAC	1 cut(s)	1248
ApeKI	GCWGC	9 cut(s)	518, 629, 796, 821, 860, 863, 983, 1565, 2141
ApoI	RAATTY	7 cut(s)	106, 805, 1027, 1072, 1230, 1605, 1765
ArsI	GACNNNNNNTTYG	2 cut(s)	1251, 1283
AseI	ATTAAT	1 cut(s)	183
Asp700I	GAANNNNTTC	2 cut(s)	728, 2226
AspLEI	GCGC	3 cut(s)	317, 597, 1348
AspS9I	GGNCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
AsuHPI	GGTGA	5 cut(s)	1467, 1627, 1736, 1847, 2105
AvaI	CYCGRG	2 cut(s)	116, 1660
AvaII	GGWCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
AxyI	CCTNAGG	1 cut(s)	1263
BaeGI	GKGCMC	1 cut(s)	1252
BalI	TGGCCA	1 cut(s)	1160
BamHI	GGATCC	1 cut(s)	2134
BanII	GRGCYC	4 cut(s)	785, 935, 1355, 1849
BauI	CACGAG	2 cut(s)	1803, 2181
BbsI	GAAGAC	3 cut(s)	497, 731, 882
Bbv12I	GWGCWC	4 cut(s)	785, 1252, 1355, 1456
BbvI	GCAGC	9 cut(s)	505, 616, 808, 808, 847, 875, 970, 1552, 2153
BccI	CCATC	2 cut(s)	1574, 2201
BceAI	ACGGC	2 cut(s)	1332, 1615
BciT130I	CCWGG	2 cut(s)	340, 1737
BciVI	GTATCC	1 cut(s)	1296
BcoDI	GTCTC	5 cut(s)	134, 233, 510, 1033, 1307
BfaI	CTAG	5 cut(s)	75, 450, 552, 1245, 2076
BfmI	CTRYAG	3 cut(s)	738, 756, 861
BfuAI	ACCTGC	1 cut(s)	763
BfuI	GTATCC	1 cut(s)	1296
BglI	GCCNNNNNGGC	1 cut(s)	2277
BisI	GCNGC	9 cut(s)	519, 630, 797, 822, 861, 864, 984, 1566, 2142
BlpI	GCTNAGC	1 cut(s)	1354
BlsI	GCNGC	9 cut(s)	520, 631, 798, 823, 862, 865, 985, 1567, 2143
BmcAI	AGTACT	1 cut(s)	210
Bme1390I	CCNGG	2 cut(s)	340, 1737
Bme18I	GGWCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
BmeT110I	CYCGRG	2 cut(s)	116, 1660
BmgT120I	GGNCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
BmrFI	CCNGG	2 cut(s)	340, 1737
BmrI	ACTGGG	2 cut(s)	438, 1702
BmsI	GCATC	8 cut(s)	10, 808, 980, 982, 1629, 1882, 2074, 2176
BmuI	ACTGGG	2 cut(s)	438, 1702
BpiI	GAAGAC	3 cut(s)	497, 731, 882
BplI	GAGNNNNNCTC	2 cut(s)	116, 148
BpmI	CTGGAG	1 cut(s)	1440
Bpu10I	CCTNAGC	1 cut(s)	33
Bpu1102I	GCTNAGC	1 cut(s)	1354
BpuEI	CTTGAG	2 cut(s)	143, 1759
BsaI	GGTCTC	1 cut(s)	134
BsaJI	CCNNGG	3 cut(s)	1161, 2019, 2281
BsaWI	WCCGGW	1 cut(s)	1288
Bsc4I	CCNNNNNNNGG	6 cut(s)	429, 430, 1276, 1561, 1832, 1872
Bse1I	ACTGG	4 cut(s)	433, 1697, 1865, 2192
Bse21I	CCTNAGG	1 cut(s)	1263
Bse3DI	GCAATG	1 cut(s)	655
BseBI	CCWGG	2 cut(s)	340, 1737
BseDI	CCNNGG	3 cut(s)	1161, 2019, 2281
BseGI	GGATG	4 cut(s)	280, 997, 1117, 1815
BseLI	CCNNNNNNNGG	6 cut(s)	429, 430, 1276, 1561, 1832, 1872
BseMI	GCAATG	1 cut(s)	655
BseMII	CTCAG	5 cut(s)	36, 1188, 1285, 1368, 1932
BseNI	ACTGG	4 cut(s)	433, 1697, 1865, 2192
BseRI	GAGGAG	2 cut(s)	1956, 2060
BseSI	GKGCMC	1 cut(s)	1252
BseXI	GCAGC	9 cut(s)	505, 616, 808, 808, 847, 875, 970, 1552, 2153
BseYI	CCCAGC	3 cut(s)	591, 792, 881
BsgI	GTGCAG	2 cut(s)	327, 504
Bsh1236I	CGCG	2 cut(s)	597, 1348
BshFI	GGCC	6 cut(s)	338, 362, 1147, 1160, 1735, 2280
BsiHKAI	GWGCWC	4 cut(s)	785, 1252, 1355, 1456
BsiHKCI	CYCGRG	2 cut(s)	116, 1660
BsiSI	CCGG	1 cut(s)	1289
BslFI	GGGAC	4 cut(s)	99, 406, 1849, 2057
BslI	CCNNNNNNNGG	6 cut(s)	429, 430, 1276, 1561, 1832, 1872
BsmAI	GTCTC	5 cut(s)	134, 233, 510, 1033, 1307
BsmBI	CGTCTC	2 cut(s)	510, 1307
BsmFI	GGGAC	4 cut(s)	99, 406, 1849, 2057
BsmI	GAATGC	1 cut(s)	586
BsnI	GGCC	6 cut(s)	338, 362, 1147, 1160, 1735, 2280
Bso31I	GGTCTC	1 cut(s)	134
BsoBI	CYCGRG	2 cut(s)	116, 1660
Bsp1286I	GDGCHC	6 cut(s)	785, 935, 1252, 1355, 1456, 1849
Bsp1407I	TGTACA	1 cut(s)	394
Bsp143I	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
Bsp1720I	GCTNAGC	1 cut(s)	1354
Bsp19I	CCATGG	1 cut(s)	1161
BspACI	CCGC	6 cut(s)	23, 597, 995, 1036, 1348, 1642
BspANI	GGCC	6 cut(s)	338, 362, 1147, 1160, 1735, 2280
BspCNI	CTCAG	5 cut(s)	37, 1189, 1284, 1367, 1933
BspFNI	CGCG	2 cut(s)	597, 1348
BspMAI	CTGCAG	2 cut(s)	760, 865
BspMI	ACCTGC	1 cut(s)	763
BspPI	GGATC	5 cut(s)	1109, 1533, 1533, 2129, 2142
BspQI	GCTCTTC	1 cut(s)	790
BspTNI	GGTCTC	1 cut(s)	134
BsrBI	CCGCTC	1 cut(s)	1036
BsrDI	GCAATG	1 cut(s)	655
BsrGI	TGTACA	1 cut(s)	394
BsrI	ACTGG	4 cut(s)	433, 1697, 1865, 2192
BssECI	CCNNGG	3 cut(s)	1161, 2019, 2281
BssMI	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
BssSI	CACGAG	2 cut(s)	1803, 2181
BssT1I	CCWWGG	3 cut(s)	1161, 2019, 2281
Bst2BI	CACGAG	2 cut(s)	1803, 2181
Bst2UI	CCWGG	2 cut(s)	340, 1737
Bst4CI	ACNGT	9 cut(s)	496, 527, 857, 967, 1044, 1130, 1396, 1451, 1996
Bst6I	CTCTTC	4 cut(s)	172, 790, 1412, 2217
BstAPI	GCANNNNNTGC	2 cut(s)	629, 657
BstAUI	TGTACA	1 cut(s)	394
BstC8I	GCNNGC	3 cut(s)	457, 1005, 1374
BstDSI	CCRYGG	1 cut(s)	1161
BstENI	CCTNNNNNAGG	1 cut(s)	1274
BstF5I	GGATG	4 cut(s)	280, 997, 1117, 1815
BstFNI	CGCG	2 cut(s)	597, 1348
BstHHI	GCGC	3 cut(s)	317, 597, 1348
BstKTI	GATC	6 cut(s)	1117, 1488, 1528, 1541, 1620, 2137
BstMAI	GTCTC	5 cut(s)	134, 233, 510, 1033, 1307
BstMBI	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
BstNI	CCWGG	2 cut(s)	340, 1737
BstNSI	RCATGY	1 cut(s)	2271
BstSCI	CCNGG	2 cut(s)	338, 1735
BstSFI	CTRYAG	3 cut(s)	738, 756, 861
BstSLI	GKGCMC	1 cut(s)	1252
BstUI	CGCG	2 cut(s)	597, 1348
BstV1I	GCAGC	9 cut(s)	505, 616, 808, 808, 847, 875, 970, 1552, 2153
BstV2I	GAAGAC	3 cut(s)	497, 731, 882
BstX2I	RGATCY	2 cut(s)	1538, 2134
BstYI	RGATCY	2 cut(s)	1538, 2134
Bsu36I	CCTNAGG	1 cut(s)	1263
BsuI	GTATCC	1 cut(s)	1296
BsuRI	GGCC	6 cut(s)	338, 362, 1147, 1160, 1735, 2280
BtgI	CCRYGG	1 cut(s)	1161
BtsCI	GGATG	4 cut(s)	280, 997, 1117, 1815
BtsI	GCAGTG	1 cut(s)	423
BtsIMutI	CAGTG	5 cut(s)	423, 447, 1456, 2001, 2199
BveI	ACCTGC	1 cut(s)	763
Cac8I	GCNNGC	3 cut(s)	457, 1005, 1374
CaiI	CAGNNNCTG	1 cut(s)	1361
CfoI	GCGC	3 cut(s)	317, 597, 1348
Cfr13I	GGNCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
CseI	GACGC	1 cut(s)	492
Csp6I	GTAC	6 cut(s)	209, 395, 426, 561, 1683, 2265
CviAII	CATG	6 cut(s)	274, 385, 1162, 1915, 1949, 2268
CviQI	GTAC	6 cut(s)	209, 395, 426, 561, 1683, 2265
DpnI	GATC	6 cut(s)	1116, 1487, 1527, 1540, 1619, 2136
DpnII	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
DraI	TTTAAA	2 cut(s)	1764, 1990
EaeI	YGGCCR	2 cut(s)	1158, 2278
Eam1104I	CTCTTC	4 cut(s)	172, 790, 1412, 2217
EarI	CTCTTC	4 cut(s)	172, 790, 1412, 2217
Ecl136II	GAGCTC	2 cut(s)	783, 1353
Eco130I	CCWWGG	3 cut(s)	1161, 2019, 2281
Eco147I	AGGCCT	3 cut(s)	338, 1147, 1735
Eco24I	GRGCYC	4 cut(s)	785, 935, 1355, 1849
Eco31I	GGTCTC	1 cut(s)	134
Eco47I	GGWCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
Eco53kI	GAGCTC	2 cut(s)	783, 1353
Eco57I	CTGAAG	2 cut(s)	876, 1383
Eco81I	CCTNAGG	1 cut(s)	1263
Eco88I	CYCGRG	2 cut(s)	116, 1660
EcoICRI	GAGCTC	2 cut(s)	783, 1353
EcoNI	CCTNNNNNAGG	1 cut(s)	1274
EcoO109I	RGGNCCY	3 cut(s)	588, 1267, 2071
EcoRI	GAATTC	2 cut(s)	805, 1605
EcoRII	CCWGG	2 cut(s)	338, 1735
EcoT14I	CCWWGG	3 cut(s)	1161, 2019, 2281
EcoT38I	GRGCYC	4 cut(s)	785, 935, 1355, 1849
ErhI	CCWWGG	3 cut(s)	1161, 2019, 2281
Esp3I	CGTCTC	2 cut(s)	510, 1307
FaeI	CATG	6 cut(s)	277, 388, 1165, 1918, 1952, 2271
FalI	AAGNNNNNCTT	2 cut(s)	716, 748
FaqI	GGGAC	4 cut(s)	99, 406, 1849, 2057
FatI	CATG	6 cut(s)	273, 384, 1161, 1914, 1948, 2267
FauI	CCCGC	1 cut(s)	1635
FauNDI	CATATG	2 cut(s)	619, 1570
Fnu4HI	GCNGC	9 cut(s)	519, 630, 797, 822, 861, 864, 984, 1566, 2142
FokI	GGATG	4 cut(s)	267, 1004, 1124, 1822
FriOI	GRGCYC	4 cut(s)	785, 935, 1355, 1849
Fsp4HI	GCNGC	9 cut(s)	519, 630, 797, 822, 861, 864, 984, 1566, 2142
FspBI	CTAG	5 cut(s)	75, 450, 552, 1245, 2076
GlaI	GCGC	3 cut(s)	316, 596, 1347
GluI	GCNGC	9 cut(s)	519, 630, 797, 822, 861, 864, 984, 1566, 2142
GsaI	CCCAGC	3 cut(s)	595, 796, 885
GsuI	CTGGAG	1 cut(s)	1440
HaeIII	GGCC	6 cut(s)	338, 362, 1147, 1160, 1735, 2280
HapII	CCGG	1 cut(s)	1289
HgaI	GACGC	1 cut(s)	492
HhaI	GCGC	3 cut(s)	317, 597, 1348
Hin1II	CATG	6 cut(s)	277, 388, 1165, 1918, 1952, 2271
Hin6I	GCGC	3 cut(s)	315, 595, 1346
HinP1I	GCGC	3 cut(s)	315, 595, 1346
HincII	GTYRAC	3 cut(s)	144, 543, 1435
HindII	GTYRAC	3 cut(s)	144, 543, 1435
HinfI	GANTC	5 cut(s)	1066, 1813, 1876, 2035, 2227
HpaII	CCGG	1 cut(s)	1289
HphI	GGTGA	5 cut(s)	1467, 1627, 1736, 1847, 2105
Hpy166II	GTNNAC	6 cut(s)	144, 160, 543, 1250, 1435, 1938
Hpy8I	GTNNAC	6 cut(s)	144, 160, 543, 1250, 1435, 1938
Hpy99I	CGWCG	1 cut(s)	2258
HpyAV	CCTTC	4 cut(s)	305, 624, 1072, 2160
HpyCH4III	ACNGT	9 cut(s)	496, 527, 857, 967, 1044, 1130, 1396, 1451, 1996
HpyCH4IV	ACGT	1 cut(s)	1491
HpySE526I	ACGT	1 cut(s)	1491
Hsp92II	CATG	6 cut(s)	277, 388, 1165, 1918, 1952, 2271
HspAI	GCGC	3 cut(s)	315, 595, 1346
KflI	GGGWCCC	1 cut(s)	2071
Kzo9I	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
LguI	GCTCTTC	1 cut(s)	790
LmnI	GCTCC	8 cut(s)	754, 878, 1175, 1350, 1459, 1969, 2116, 2155
Lsp1109I	GCAGC	9 cut(s)	505, 616, 808, 808, 847, 875, 970, 1552, 2153
LweI	GCATC	8 cut(s)	10, 808, 980, 982, 1629, 1882, 2074, 2176
MaeI	CTAG	5 cut(s)	75, 450, 552, 1245, 2076
MaeII	ACGT	1 cut(s)	1491
MaeIII	GTNAC	6 cut(s)	217, 830, 946, 977, 1473, 2078
MalI	GATC	6 cut(s)	1116, 1487, 1527, 1540, 1619, 2136
MbiI	CCGCTC	1 cut(s)	1036
MboI	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
MfeI	CAATTG	1 cut(s)	1047
MflI	RGATCY	2 cut(s)	1538, 2134
MhlI	GDGCHC	6 cut(s)	785, 935, 1252, 1355, 1456, 1849
MlsI	TGGCCA	1 cut(s)	1160
MluNI	TGGCCA	1 cut(s)	1160
MlyI	GAGTC	1 cut(s)	1075
Mox20I	TGGCCA	1 cut(s)	1160
MroXI	GAANNNNTTC	2 cut(s)	728, 2226
MscI	TGGCCA	1 cut(s)	1160
MseI	TTAA	8 cut(s)	183, 246, 321, 1635, 1656, 1763, 1785, 1989
MslI	CAYNNNNRTG	2 cut(s)	528, 944
Msp20I	TGGCCA	1 cut(s)	1160
MspI	CCGG	1 cut(s)	1289
MspR9I	CCNGG	2 cut(s)	340, 1737
MunI	CAATTG	1 cut(s)	1047
Mva1269I	GAATGC	1 cut(s)	586
MvaI	CCWGG	2 cut(s)	340, 1737
MvnI	CGCG	2 cut(s)	597, 1348
NcoI	CCATGG	1 cut(s)	1161
NdeI	CATATG	2 cut(s)	619, 1570
NdeII	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
NlaIII	CATG	6 cut(s)	277, 388, 1165, 1918, 1952, 2271
NmuCI	GTSAC	4 cut(s)	946, 977, 1473, 2078
NspI	RCATGY	1 cut(s)	2271
OliI	CACNNNNGTG	2 cut(s)	528, 944
PaeR7I	CTCGAG	1 cut(s)	1660
PaqCI	CACCTGC	1 cut(s)	763
PceI	AGGCCT	3 cut(s)	338, 1147, 1735
PciSI	GCTCTTC	1 cut(s)	790
PctI	GAATGC	1 cut(s)	586
PdmI	GAANNNNTTC	2 cut(s)	728, 2226
PfeI	GAWTC	4 cut(s)	1813, 1876, 2035, 2227
PkrI	GCNGC	9 cut(s)	520, 631, 798, 823, 862, 865, 985, 1567, 2143
PleI	GAGTC	1 cut(s)	1074
PpsI	GAGTC	1 cut(s)	1074
PpuMI	RGGWCCY	3 cut(s)	588, 1267, 2071
PshBI	ATTAAT	1 cut(s)	183
Psp124BI	GAGCTC	2 cut(s)	785, 1355
Psp1406I	AACGTT	1 cut(s)	1491
Psp5II	RGGWCCY	3 cut(s)	588, 1267, 2071
Psp6I	CCWGG	2 cut(s)	338, 1735
PspFI	CCCAGC	3 cut(s)	591, 792, 881
PspGI	CCWGG	2 cut(s)	338, 1735
PspPI	GGNCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
PspPPI	RGGWCCY	3 cut(s)	588, 1267, 2071
PstI	CTGCAG	2 cut(s)	760, 865
PstNI	CAGNNNCTG	1 cut(s)	1361
PsuI	RGATCY	2 cut(s)	1538, 2134
RsaI	GTAC	6 cut(s)	210, 396, 427, 562, 1684, 2266
RsaNI	GTAC	6 cut(s)	209, 395, 426, 561, 1683, 2265
RseI	CAYNNNNRTG	2 cut(s)	528, 944
SacI	GAGCTC	2 cut(s)	785, 1355
SapI	GCTCTTC	1 cut(s)	790
SaqAI	TTAA	8 cut(s)	183, 246, 321, 1635, 1656, 1763, 1785, 1989
SatI	GCNGC	9 cut(s)	519, 630, 797, 822, 861, 864, 984, 1566, 2142
Sau3AI	GATC	6 cut(s)	1114, 1485, 1525, 1538, 1617, 2134
Sau96I	GGNCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
ScaI	AGTACT	1 cut(s)	210
SchI	GAGTC	1 cut(s)	1075
ScrFI	CCNGG	2 cut(s)	340, 1737
SduI	GDGCHC	6 cut(s)	785, 935, 1252, 1355, 1456, 1849
SfaNI	GCATC	8 cut(s)	10, 808, 980, 982, 1629, 1882, 2074, 2176
SfcI	CTRYAG	3 cut(s)	738, 756, 861
Sfr274I	CTCGAG	1 cut(s)	1660
SinI	GGWCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
SlaI	CTCGAG	1 cut(s)	1660
SmiMI	CAYNNNNRTG	2 cut(s)	528, 944
SmlI	CTYRAG	3 cut(s)	122, 1660, 1774
SmoI	CTYRAG	3 cut(s)	122, 1660, 1774
SseBI	AGGCCT	3 cut(s)	338, 1147, 1735
SsiI	CCGC	6 cut(s)	23, 597, 995, 1036, 1348, 1642
SspMI	CTAG	5 cut(s)	75, 450, 552, 1245, 2076
SstI	GAGCTC	2 cut(s)	785, 1355
StuI	AGGCCT	3 cut(s)	338, 1147, 1735
StyD4I	CCNGG	2 cut(s)	338, 1735
StyI	CCWWGG	3 cut(s)	1161, 2019, 2281
TaaI	ACNGT	9 cut(s)	496, 527, 857, 967, 1044, 1130, 1396, 1451, 1996
TaiI	ACGT	1 cut(s)	1494
TaqI	TCGA	3 cut(s)	1528, 1603, 1661
TaqII	GACCGA	1 cut(s)	2267
TatI	WGTACW	2 cut(s)	208, 394
TfiI	GAWTC	4 cut(s)	1813, 1876, 2035, 2227
Tru1I	TTAA	8 cut(s)	183, 246, 321, 1635, 1656, 1763, 1785, 1989
Tru9I	TTAA	8 cut(s)	183, 246, 321, 1635, 1656, 1763, 1785, 1989
TscAI	CASTG	5 cut(s)	423, 447, 1456, 2001, 2199
TseFI	GTSAC	4 cut(s)	946, 977, 1473, 2078
TseI	GCWGC	9 cut(s)	518, 629, 796, 821, 860, 863, 983, 1565, 2141
Tsp45I	GTSAC	4 cut(s)	946, 977, 1473, 2078
TspDTI	ATGAA	9 cut(s)	262, 269, 491, 593, 606, 1034, 1499, 1689, 2235
TspGWI	ACGGA	7 cut(s)	213, 426, 528, 1124, 1272, 2045, 2047
TspRI	CASTG	5 cut(s)	423, 447, 1456, 2001, 2199
VneI	GTGCAC	1 cut(s)	1248
VpaK11BI	GGWCC	8 cut(s)	434, 536, 588, 642, 1267, 1552, 1863, 2071
VspI	ATTAAT	1 cut(s)	183
XagI	CCTNNNNNAGG	1 cut(s)	1274
XapI	RAATTY	7 cut(s)	106, 805, 1027, 1072, 1230, 1605, 1765
XceI	RCATGY	1 cut(s)	2271
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2121
XhoI	CTCGAG	1 cut(s)	1660
XmnI	GAANNNNTTC	2 cut(s)	728, 2226
XspI	CTAG	5 cut(s)	75, 450, 552, 1245, 2076
ZrmI	AGTACT	1 cut(s)	210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.