Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008657

Physical Location & Seq

Genomic Coordinates Forward (+)

31034620 .. 31037491

Gene Structure 2872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1217800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGGGAGAATTGAACATGGAGGACATGCCTCTGCCGGCACTTTTCGAGCAAGCTCGGAAGATCCACCTGGGGGCGACGGAGTCCGGCGACGGTGTCGATCAGGAGGAGATTAGGAAGGGATGCAGAGCCTTGGAGAAGTGCGATGAAATGATAAGCAATCTGGGTTTGTTCTCAGCCAATGAGACCAAGGAGGATATCAGCACCACCAATCTCAAATATCTTCTGGTGCCATATTATCTAGGGGAGTTAATCGAAAAGGTTGCGCAGGATGACAGGATATCGGTTCTTAAGGCTTCCCAGGCTAAGTTAAAGGAGTTTATTTCTATTTGCGAGGCAATGGAACTTGTTCCAAAAGAGGAGTTAGAAGCATCTGCAAAAGATGGCCCAAATTCACATGTTGATCGAAGGGCACTGAAGATTGCTCGATTCAAAAGGCAAAGAGCTGCAGAATCGAAATTGCTGGAAATTAAGGAGCGGAAAGAGCGAAGAGGGCGTTCAACTAAAGCATCTGCCTTGTCAACTCGTATTGAGGCAGGAGAGGAAGATGTGCTGGATGATGACGGAGAGGAGGAGCGAGAGGCTTGGTTTACTACAATCTCTTTGGCAGTTAGTAAGGCTTTTGATCTGTTGGAGATGCTAAAAAAAGAGGAAGAAATGCTTGTAGCTTTTAAGGAAAGGCAATCAAAGGATGGTGGCAAGGAATTTTATCAAGATGTTCTTGATGATCGTGCCAAGAGGGCCGAAGCTTGGCATCGTGATGCTGCAACCCGAGTGCAGTATAGTAGACCAGCAGAGCCTATCACCTGTGCAACTTTTGCTCAAGACGTTCTAGAAGGCAGAGCCAGGGTTTCGGAGATGCATGAACACAAACATCAGCCACTAATATTTGGACCGGCAAGTCTTATAGGTGGAAACCCAACAAGCGAGAGGGAAAGGATGGCGGCTCAGGTTTTCCAGCCCAGTCACAGGTTGCCGACCATGAGCATAGAGGAAGCCGGACTGAAGGAGATGGAGATAATGAATACGTGGCAAGAGAGGAACACGAAACTCATGGAAGAAGCCAATTCTGCATGGTACAAGGAACCTATGAAACCAGGACCACCACCGGAGGGGGAAGATGAAGACGATGATGCCGCTCAAGACAGGGCGAGGGCTTGGGATGACTGGAAAGATGATAATCCTCGGGGTGCTGGCAACAAGAAGCTCACCCCCTGTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0001558 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006808 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009966 GO:0009967 GO:0009987 GO:0010029 GO:0010033 GO:0010187 GO:0010646 GO:0010647 GO:0010921 GO:0019208 GO:0019220 GO:0019222 GO:0019888 GO:0019899 GO:0019900 GO:0019902 GO:0019903 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030307 GO:0031323 GO:0031399 GO:0031929 GO:0032006 GO:0032008 GO:0032268 GO:0033993 GO:0035303 GO:0035304 GO:0035556 GO:0040008 GO:0042221 GO:0043666 GO:0044424 GO:0044444 GO:0044464 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051128 GO:0051171 GO:0051174 GO:0051239 GO:0051241 GO:0051246 GO:0051336 GO:0051716 GO:0051721 GO:0060255 GO:0065007 GO:0065009 GO:0080090 GO:0097305 GO:0098772 GO:1900140 GO:1901700 GO:1902531 GO:1902533 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 map04136 map04138 map04140

Pfam Domains

Protein Families

TAP42

Protein Analysis

407

Amino Acids

45.98

Weight (kDa)

5.03

Isoelectric Point (pI)

44.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAP42	PF04177	11 - 394	9.1e-88	TAP42-like family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008657)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53000
fragaria_vesca	FvH4_7g31380 FvH4_7g31380 FvH4_7g31380
malus_domestica	MD01G1217800.v1.1 MD07G1287100.v1.1 MD07G1287400.v1.1
prunus_persica	Prupe.2G309200_v2.0.a1 Prupe.2G309200_v2.0.a1
pyrus_communis	pycom01g22790 pycom07g26040
rosa_chinensis	RchiOBHm_Chr1g0380451
rosa_laevigata	RLG00000026299
rosa_multiflora	Rmu_sc0001733.1_g000010 Rmu_sc0005371.1_g000013 Rmu_sc0006267.1_g000001
rosa_roxburghii	Rroxscaffold_4G00279350
rosa_rugosa	Rorug01G0421200
rosa_samantha	Rh1AG445700 Rh1BG401000 Rh1CG415400 Rh1DG431100
rosa_wichuraiana	Rw1G038790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	897
Acc16I	TGCGCA	1 cut(s)	264
AccB1I	GGYRCC	1 cut(s)	226
AccBSI	CCGCTC	2 cut(s)	475, 1136
AccI	GTMKAC	1 cut(s)	784
AciI	CCGC	3 cut(s)	475, 941, 1134
AclWI	GGATC	1 cut(s)	55
AcsI	RAATTY	2 cut(s)	388, 701
AcuI	CTGAAG	2 cut(s)	434, 1022
AfaI	GTAC	1 cut(s)	1076
AfiI	CCNNNNNNNGG	4 cut(s)	70, 747, 966, 1109
AflII	CTTAAG	1 cut(s)	287
AflIII	ACRYGT	1 cut(s)	394
AgsI	TTSAA	3 cut(s)	13, 430, 498
AjnI	CCWGG	4 cut(s)	66, 297, 842, 1093
AluBI	AGCT	5 cut(s)	53, 443, 665, 746, 1204
AluI	AGCT	5 cut(s)	53, 443, 665, 746, 1204
Alw26I	GTCTC	1 cut(s)	176
AlwI	GGATC	1 cut(s)	55
Ama87I	CYCGRG	2 cut(s)	768, 1182
AoxI	GGCC	2 cut(s)	382, 738
ApeKI	GCWGC	2 cut(s)	443, 761
ApoI	RAATTY	2 cut(s)	388, 701
Asp700I	GAANNNNTTC	1 cut(s)	345
AspLEI	GCGC	1 cut(s)	265
AspS9I	GGNCC	4 cut(s)	383, 738, 890, 1097
AsuHPI	GGTGA	2 cut(s)	793, 1198
AvaI	CYCGRG	2 cut(s)	768, 1182
AvaII	GGWCC	2 cut(s)	890, 1097
BaeGI	GKGCMC	1 cut(s)	412
BanI	GGYRCC	1 cut(s)	226
BbsI	GAAGAC	1 cut(s)	1128
BbvI	GCAGC	2 cut(s)	430, 748
BccI	CCATC	4 cut(s)	374, 683, 931, 1003
BcgI	CGANNNNNNTGC	2 cut(s)	242, 276
BciT130I	CCWGG	4 cut(s)	68, 299, 844, 1095
BcoDI	GTCTC	1 cut(s)	176
BfaI	CTAG	2 cut(s)	239, 830
BfmI	CTRYAG	1 cut(s)	444
BfrI	CTTAAG	1 cut(s)	287
BglI	GCCNNNNNGGC	1 cut(s)	737
BisI	GCNGC	4 cut(s)	444, 762, 942, 1134
BlsI	GCNGC	4 cut(s)	445, 763, 943, 1135
Bme1390I	CCNGG	4 cut(s)	68, 299, 844, 1095
Bme18I	GGWCC	2 cut(s)	890, 1097
BmeT110I	CYCGRG	2 cut(s)	768, 1182
BmgT120I	GGNCC	4 cut(s)	383, 738, 890, 1097
BmiI	GGNNCC	2 cut(s)	228, 1083
BmrFI	CCNGG	4 cut(s)	68, 299, 844, 1095
BmrI	ACTGGG	1 cut(s)	954
BmsI	GCATC	8 cut(s)	110, 377, 515, 624, 748, 760, 846, 1120
BmuI	ACTGGG	1 cut(s)	954
BpiI	GAAGAC	1 cut(s)	1128
Bpu10I	CCTNAGC	1 cut(s)	945
BpuEI	CTTGAG	2 cut(s)	804, 1122
BsaAI	YACGTR	1 cut(s)	1026
BsaBI	GATNNNNATC	1 cut(s)	1176
BsaI	GGTCTC	1 cut(s)	176
BsaJI	CCNNGG	6 cut(s)	67, 129, 186, 297, 843, 1181
BsaWI	WCCGGW	1 cut(s)	1105
Bsc4I	CCNNNNNNNGG	4 cut(s)	70, 747, 966, 1109
Bse118I	RCCGGY	2 cut(s)	34, 892
Bse1I	ACTGG	2 cut(s)	960, 1169
Bse3DI	GCAATG	1 cut(s)	342
Bse8I	GATNNNNATC	1 cut(s)	1176
BseBI	CCWGG	4 cut(s)	68, 299, 844, 1095
BseDI	CCNNGG	6 cut(s)	67, 129, 186, 297, 843, 1181
BseGI	GGATG	6 cut(s)	125, 274, 559, 694, 942, 1165
BseJI	GATNNNNATC	1 cut(s)	1176
BseLI	CCNNNNNNNGG	4 cut(s)	70, 747, 966, 1109
BseMI	GCAATG	1 cut(s)	342
BseMII	CTCAG	2 cut(s)	186, 959
BseNI	ACTGG	2 cut(s)	960, 1169
BseRI	GAGGAG	4 cut(s)	119, 371, 581, 584
BseSI	GKGCMC	1 cut(s)	412
BseXI	GCAGC	2 cut(s)	430, 748
BsgI	GTGCAG	1 cut(s)	794
BshFI	GGCC	2 cut(s)	384, 740
BshNI	GGYRCC	1 cut(s)	226
BsiHKCI	CYCGRG	2 cut(s)	768, 1182
BsiSI	CCGG	5 cut(s)	35, 84, 893, 996, 1106
BslI	CCNNNNNNNGG	4 cut(s)	70, 747, 966, 1109
BsmAI	GTCTC	1 cut(s)	176
BsnI	GGCC	2 cut(s)	384, 740
Bso31I	GGTCTC	1 cut(s)	176
BsoBI	CYCGRG	2 cut(s)	768, 1182
Bsp1286I	GDGCHC	1 cut(s)	412
Bsp143I	GATC	5 cut(s)	60, 97, 400, 622, 724
BspACI	CCGC	3 cut(s)	475, 941, 1134
BspANI	GGCC	2 cut(s)	384, 740
BspCNI	CTCAG	2 cut(s)	185, 958
BspLI	GGNNCC	2 cut(s)	228, 1083
BspMAI	CTGCAG	1 cut(s)	448
BspPI	GGATC	1 cut(s)	55
BspT107I	GGYRCC	1 cut(s)	226
BspTI	CTTAAG	1 cut(s)	287
BspTNI	GGTCTC	1 cut(s)	176
BsrBI	CCGCTC	2 cut(s)	475, 1136
BsrDI	GCAATG	1 cut(s)	342
BsrFI	RCCGGY	2 cut(s)	34, 892
BsrI	ACTGG	2 cut(s)	960, 1169
BssAI	RCCGGY	2 cut(s)	34, 892
BssECI	CCNNGG	6 cut(s)	67, 129, 186, 297, 843, 1181
BssMI	GATC	5 cut(s)	60, 97, 400, 622, 724
BssT1I	CCWWGG	2 cut(s)	129, 186
Bst2UI	CCWGG	4 cut(s)	68, 299, 844, 1095
Bst4CI	ACNGT	1 cut(s)	92
Bst6I	CTCTTC	1 cut(s)	481
BstAFI	CTTAAG	1 cut(s)	287
BstAPI	GCANNNNNTGC	1 cut(s)	815
BstBAI	YACGTR	1 cut(s)	1026
BstC8I	GCNNGC	3 cut(s)	36, 51, 1192
BstDEI	CTNAG	3 cut(s)	172, 303, 945
BstF5I	GGATG	6 cut(s)	125, 274, 559, 694, 942, 1165
BstHHI	GCGC	1 cut(s)	265
BstKTI	GATC	5 cut(s)	63, 100, 403, 625, 727
BstMAI	GTCTC	1 cut(s)	176
BstMBI	GATC	5 cut(s)	60, 97, 400, 622, 724
BstMWI	GCNNNNNNNGC	6 cut(s)	299, 481, 490, 737, 815, 1067
BstNI	CCWGG	4 cut(s)	68, 299, 844, 1095
BstNSI	RCATGY	2 cut(s)	28, 398
BstSCI	CCNGG	4 cut(s)	66, 297, 842, 1093
BstSFI	CTRYAG	1 cut(s)	444
BstSLI	GKGCMC	1 cut(s)	412
BstV1I	GCAGC	2 cut(s)	430, 748
BstV2I	GAAGAC	1 cut(s)	1128
BstX2I	RGATCY	1 cut(s)	60
BstYI	RGATCY	1 cut(s)	60
BsuRI	GGCC	2 cut(s)	384, 740
BtgZI	GCGATG	1 cut(s)	156
BtsCI	GGATG	6 cut(s)	125, 274, 559, 694, 942, 1165
BtsIMutI	CAGTG	1 cut(s)	410
Cac8I	GCNNGC	3 cut(s)	36, 51, 1192
CfoI	GCGC	1 cut(s)	265
Cfr10I	RCCGGY	2 cut(s)	34, 892
Cfr13I	GGNCC	4 cut(s)	383, 738, 890, 1097
Csp6I	GTAC	1 cut(s)	1075
CviAII	CATG	7 cut(s)	16, 25, 395, 860, 979, 1051, 1071
CviQI	GTAC	1 cut(s)	1075
DdeI	CTNAG	3 cut(s)	172, 303, 945
DpnI	GATC	5 cut(s)	62, 99, 402, 624, 726
DpnII	GATC	5 cut(s)	60, 97, 400, 622, 724
DrdI	GACNNNNNNGTC	1 cut(s)	897
DseDI	GACNNNNNNGTC	1 cut(s)	897
Eam1104I	CTCTTC	1 cut(s)	481
EarI	CTCTTC	1 cut(s)	481
Eco130I	CCWWGG	2 cut(s)	129, 186
Eco31I	GGTCTC	1 cut(s)	176
Eco32I	GATATC	2 cut(s)	196, 279
Eco47I	GGWCC	2 cut(s)	890, 1097
Eco57I	CTGAAG	2 cut(s)	434, 1022
Eco88I	CYCGRG	2 cut(s)	768, 1182
EcoRII	CCWGG	4 cut(s)	66, 297, 842, 1093
EcoRV	GATATC	2 cut(s)	196, 279
EcoT14I	CCWWGG	2 cut(s)	129, 186
EcoT22I	ATGCAT	1 cut(s)	861
ErhI	CCWWGG	2 cut(s)	129, 186
FaeI	CATG	7 cut(s)	19, 28, 398, 863, 982, 1054, 1074
FalI	AAGNNNNNCTT	4 cut(s)	642, 674, 702, 734
FatI	CATG	7 cut(s)	15, 24, 394, 859, 978, 1050, 1070
FblI	GTMKAC	1 cut(s)	784
Fnu4HI	GCNGC	4 cut(s)	444, 762, 942, 1134
FokI	GGATG	6 cut(s)	132, 281, 566, 701, 949, 1172
Fsp4HI	GCNGC	4 cut(s)	444, 762, 942, 1134
FspBI	CTAG	2 cut(s)	239, 830
FspI	TGCGCA	1 cut(s)	264
GlaI	GCGC	1 cut(s)	264
GluI	GCNGC	4 cut(s)	444, 762, 942, 1134
HaeIII	GGCC	2 cut(s)	384, 740
HapII	CCGG	5 cut(s)	35, 84, 893, 996, 1106
HhaI	GCGC	1 cut(s)	265
Hin1II	CATG	7 cut(s)	19, 28, 398, 863, 982, 1054, 1074
Hin6I	GCGC	1 cut(s)	263
HinP1I	GCGC	1 cut(s)	263
HincII	GTYRAC	1 cut(s)	519
HindII	GTYRAC	1 cut(s)	519
HindIII	AAGCTT	1 cut(s)	744
HinfI	GANTC	3 cut(s)	80, 426, 449
HpaII	CCGG	5 cut(s)	35, 84, 893, 996, 1106
HphI	GGTGA	2 cut(s)	793, 1198
Hpy166II	GTNNAC	3 cut(s)	519, 588, 785
Hpy188I	TCNGA	2 cut(s)	57, 853
Hpy188III	TCNNGA	7 cut(s)	101, 710, 719, 755, 821, 830, 1139
Hpy8I	GTNNAC	3 cut(s)	519, 588, 785
Hpy99I	CGWCG	2 cut(s)	79, 92
HpyAV	CCTTC	4 cut(s)	109, 399, 827, 997
HpyCH4III	ACNGT	1 cut(s)	92
HpyCH4IV	ACGT	2 cut(s)	825, 1025
HpyCH4V	TGCA	8 cut(s)	123, 374, 446, 764, 775, 809, 859, 1070
HpyF10VI	GCNNNNNNNGC	6 cut(s)	299, 481, 490, 737, 815, 1067
HpyF3I	CTNAG	3 cut(s)	172, 303, 945
HpySE526I	ACGT	2 cut(s)	825, 1025
Hsp92II	CATG	7 cut(s)	19, 28, 398, 863, 982, 1054, 1074
HspAI	GCGC	1 cut(s)	263
KroI	GCCGGC	1 cut(s)	34
KroNI	GCCGGC	1 cut(s)	36
Kzo9I	GATC	5 cut(s)	60, 97, 400, 622, 724
LmnI	GCTCC	2 cut(s)	472, 571
Lsp1109I	GCAGC	2 cut(s)	430, 748
LweI	GCATC	8 cut(s)	110, 377, 515, 624, 748, 760, 846, 1120
MaeI	CTAG	2 cut(s)	239, 830
MaeII	ACGT	2 cut(s)	825, 1025
MaeIII	GTNAC	1 cut(s)	962
MalI	GATC	5 cut(s)	62, 99, 402, 624, 726
MbiI	CCGCTC	2 cut(s)	475, 1136
MboI	GATC	5 cut(s)	60, 97, 400, 622, 724
MboII	GAAGA	9 cut(s)	70, 212, 427, 498, 554, 662, 1067, 1127, 1133
MflI	RGATCY	1 cut(s)	60
MhlI	GDGCHC	1 cut(s)	412
MluCI	AATT	6 cut(s)	8, 388, 455, 465, 701, 1063
MlyI	GAGTC	1 cut(s)	89
MmeI	TCCRAC	1 cut(s)	609
Mph1103I	ATGCAT	1 cut(s)	861
MroNI	GCCGGC	1 cut(s)	34
MroXI	GAANNNNTTC	1 cut(s)	345
MseI	TTAA	5 cut(s)	248, 288, 308, 468, 669
MslI	CAYNNNNRTG	1 cut(s)	756
MspCI	CTTAAG	1 cut(s)	287
MspI	CCGG	5 cut(s)	35, 84, 893, 996, 1106
MspR9I	CCNGG	4 cut(s)	68, 299, 844, 1095
MvaI	CCWGG	4 cut(s)	68, 299, 844, 1095
MwoI	GCNNNNNNNGC	6 cut(s)	299, 481, 490, 737, 815, 1067
NaeI	GCCGGC	1 cut(s)	36
NdeII	GATC	5 cut(s)	60, 97, 400, 622, 724
NgoMIV	GCCGGC	1 cut(s)	34
NlaIII	CATG	7 cut(s)	19, 28, 398, 863, 982, 1054, 1074
NlaIV	GGNNCC	2 cut(s)	228, 1083
NmuCI	GTSAC	1 cut(s)	962
NsbI	TGCGCA	1 cut(s)	264
NsiI	ATGCAT	1 cut(s)	861
NspI	RCATGY	2 cut(s)	28, 398
PciI	ACATGT	1 cut(s)	394
PdiI	GCCGGC	1 cut(s)	36
PdmI	GAANNNNTTC	1 cut(s)	345
PfeI	GAWTC	2 cut(s)	426, 449
PflFI	GACNNNGTC	2 cut(s)	79, 92
PkrI	GCNGC	4 cut(s)	445, 763, 943, 1135
PleI	GAGTC	1 cut(s)	88
PpsI	GAGTC	1 cut(s)	88
Ppu21I	YACGTR	1 cut(s)	1026
PscI	ACATGT	1 cut(s)	394
Psp6I	CCWGG	4 cut(s)	66, 297, 842, 1093
PspGI	CCWGG	4 cut(s)	66, 297, 842, 1093
PspN4I	GGNNCC	2 cut(s)	228, 1083
PspPI	GGNCC	4 cut(s)	383, 738, 890, 1097
PstI	CTGCAG	1 cut(s)	448
PsuI	RGATCY	1 cut(s)	60
PsyI	GACNNNGTC	2 cut(s)	79, 92
RsaI	GTAC	1 cut(s)	1076
RsaNI	GTAC	1 cut(s)	1075
RseI	CAYNNNNRTG	1 cut(s)	756
SaqAI	TTAA	5 cut(s)	248, 288, 308, 468, 669
SatI	GCNGC	4 cut(s)	444, 762, 942, 1134
Sau3AI	GATC	5 cut(s)	60, 97, 400, 622, 724
Sau96I	GGNCC	4 cut(s)	383, 738, 890, 1097
SchI	GAGTC	1 cut(s)	89
ScrFI	CCNGG	4 cut(s)	68, 299, 844, 1095
SduI	GDGCHC	1 cut(s)	412
SfaNI	GCATC	8 cut(s)	110, 377, 515, 624, 748, 760, 846, 1120
SfcI	CTRYAG	1 cut(s)	444
SinI	GGWCC	2 cut(s)	890, 1097
SmiMI	CAYNNNNRTG	1 cut(s)	756
SmlI	CTYRAG	3 cut(s)	287, 819, 1137
SmoI	CTYRAG	3 cut(s)	287, 819, 1137
Sse9I	AATT	6 cut(s)	8, 388, 455, 465, 701, 1063
SsiI	CCGC	3 cut(s)	475, 941, 1134
SspI	AATATT	1 cut(s)	885
SspMI	CTAG	2 cut(s)	239, 830
StyD4I	CCNGG	4 cut(s)	66, 297, 842, 1093
StyI	CCWWGG	2 cut(s)	129, 186
TaaI	ACNGT	1 cut(s)	92
TaiI	ACGT	2 cut(s)	828, 1028
TaqI	TCGA	6 cut(s)	45, 96, 252, 403, 424, 452
TasI	AATT	6 cut(s)	8, 388, 455, 465, 701, 1063
TauI	GCSGC	2 cut(s)	944, 1136
TfiI	GAWTC	2 cut(s)	426, 449
Tru1I	TTAA	5 cut(s)	248, 288, 308, 468, 669
Tru9I	TTAA	5 cut(s)	248, 288, 308, 468, 669
TscAI	CASTG	1 cut(s)	417
TseFI	GTSAC	1 cut(s)	962
TseI	GCWGC	2 cut(s)	443, 761
Tsp45I	GTSAC	1 cut(s)	962
TspDTI	ATGAA	5 cut(s)	159, 876, 1034, 1103, 1134
TspGWI	ACGGA	2 cut(s)	92, 576
TspRI	CASTG	1 cut(s)	417
Tth111I	GACNNNGTC	2 cut(s)	79, 92
Vha464I	CTTAAG	1 cut(s)	287
VpaK11BI	GGWCC	2 cut(s)	890, 1097
XapI	RAATTY	2 cut(s)	388, 701
XbaI	TCTAGA	1 cut(s)	829
XceI	RCATGY	2 cut(s)	28, 398
XmiI	GTMKAC	1 cut(s)	784
XmnI	GAANNNNTTC	1 cut(s)	345
XspI	CTAG	2 cut(s)	239, 830
Zsp2I	ATGCAT	1 cut(s)	861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.