Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013722

Physical Location & Seq

Genomic Coordinates Forward (+)

31286401 .. 31291940

Gene Structure 5540 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1220800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGAATGGAAAGATGAGGCCACGGAGTTTCGTCATCGAGACCAAGAATGCCCGGCGACAACGACTCAGAGTCCGACGACTGAAGTACACGTGTCAGGCGAAGAAGATCCAGGTGCAGATCACCGGCGGCAGCGGCCAGAAACATGATTCCGAAGATTGCAAGACATTGGAGTTGAAGGAACAAGAGAAAGATGTTGGGAGTTCTATGGAGATATTGTCGGTGTCATTTTCGGCATCGAAAAATGACGTCGTGTTGTCTAGTGGATCCGTGGCCGGATCAGATGAAGGCGGAGAGAAAAGGAGTGGTGAGGGTTCGGAAGAGAGGGGAGGGTATGGCATCGTCTCGGTGAAGGGGATGAGGAAGGAGATGGAAGATGCAGTGAGAGCGGAGGTAGGGTTTGGGAAGTCTGACTTTTACGGGGTGTATGACGGGCACGGTGGGGCTGACGTGGCTGGGGCTTGTAGGGAGAGGATGCATGAGGTGGTGGCGGAGGTGGTGGAAATGGAAGGGACTAAAGGTGATGCGATTGATTGGGAGACGGTGATGGAGGGGTGTTTTGAGAAAATGGATGTGGAGGTGAGTGATATTGCAGCGGCGAGGACGGTTGGGGCGACAGCGGTGGTGGCTTTGGTGATGGAGGAGCAGGTGGTGGTGGCCAATTGTGGCGATTCTAGGGCTGTTTTGTCGAGAGGTGGTGTTGCCTTGGCCTTGTCCAATGACCATAAGCCGGACAGAGTGGATGAGTTGAAGAGAATTGAAGCTGCAGGTGGGAGAGTCATCAACTGGAACGGAAGCCGTGTACTTGGGGTGCTTGCCACTTCCAGATCAATAGGTGACCATTACCTGAGGCCATATGTGATATCAAGACCAGAGGTGACGGTGACCAGGCGAACCGACCAGGATGAATTCCTCATCCTTGCCAGTGACGGGCTGTGGGATGTTATATCAAACGACGTAGCATGTCAGGTTGTTAAAAAGTGTCTTCGTGGCCGAATGAAGAGAATATCCATTCACGACGAACATCGTGTTCTCCTAAATGTAGGAAGTCGCACATCTGAGGCAGCGGCAGTCTTGGTTGACCTAGCTATGGCTCGGGGAAGCAGAGATAACATTAGCGTGATTGTGGTCGACCTCACAAAACCCAGCTCGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010030 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:0140096 GO:1900140 GO:1901419 GO:1901420 GO:1901564 GO:1905957 GO:1905958 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

385

Amino Acids

41.7

Weight (kDa)

6.24

Isoelectric Point (pI)

41.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	111 - 371	1.3e-63	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013722)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g31810
malus_domestica	MD01G1220800.v1.1 MD07G1291000.v1.1
prunus_persica	Prupe.2G312300_v2.0.a1
pyrus_communis	pycom01g23030 pycom07g26360
rosa_chinensis	RchiOBHm_Chr1g0381041
rosa_laevigata	RLG00000026250
rosa_multiflora	Rmu_co8392071.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278830
rosa_rugosa	Rorug01G0426700 Rorug01G0426800 Rorug01G0426900
rosa_samantha	Rh1AG450500 Rh1BG406800 Rh1CG420600 Rh1DG437300
rosa_wichuraiana	Rw1G039260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	634, 755
AatII	GACGTC	1 cut(s)	249
Acc36I	ACCTGC	2 cut(s)	634, 755
AccBSI	CCGCTC	1 cut(s)	386
AccI	GTMKAC	1 cut(s)	1128
AciI	CCGC	8 cut(s)	126, 132, 288, 386, 488, 593, 617, 1064
AclWI	GGATC	4 cut(s)	100, 258, 271, 283
AcoI	YGGCCR	4 cut(s)	133, 270, 654, 988
AcsI	RAATTY	1 cut(s)	905
AcuI	CTGAAG	1 cut(s)	101
AcvI	CACGTG	1 cut(s)	90
AcyI	GRCGYC	1 cut(s)	246
AfaI	GTAC	2 cut(s)	86, 801
AfiI	CCNNNNNNNGG	3 cut(s)	727, 927, 1087
AflIII	ACRYGT	2 cut(s)	87, 89
AgsI	TTSAA	3 cut(s)	175, 748, 758
AhdI	GACNNNNNGTC	1 cut(s)	68
AjiI	CACGTC	1 cut(s)	448
AjnI	CCWGG	3 cut(s)	108, 884, 897
AluBI	AGCT	3 cut(s)	761, 1085, 1146
AluI	AGCT	3 cut(s)	761, 1085, 1146
Alw26I	GTCTC	3 cut(s)	32, 346, 530
AlwI	GGATC	4 cut(s)	100, 258, 271, 283
Ama87I	CYCGRG	1 cut(s)	1092
AoxI	GGCC	7 cut(s)	17, 133, 270, 654, 705, 848, 988
ApeKI	GCWGC	4 cut(s)	129, 590, 761, 1061
ApoI	RAATTY	1 cut(s)	905
AsuC2I	CCSGG	1 cut(s)	52
AvaI	CYCGRG	1 cut(s)	1092
AxyI	CCTNAGG	1 cut(s)	845
BaeGI	GKGCMC	1 cut(s)	435
BalI	TGGCCA	1 cut(s)	656
BamHI	GGATCC	1 cut(s)	263
BbrPI	CACGTG	1 cut(s)	90
BbsI	GAAGAC	1 cut(s)	974
BbvI	GCAGC	4 cut(s)	141, 602, 748, 1073
BccI	CCATC	3 cut(s)	361, 538, 628
BceAI	ACGGC	1 cut(s)	780
BciT130I	CCWGG	3 cut(s)	110, 886, 899
BcnI	CCSGG	1 cut(s)	52
BcoDI	GTCTC	3 cut(s)	32, 346, 530
BfaI	CTAG	3 cut(s)	258, 672, 1082
BfmI	CTRYAG	2 cut(s)	762, 1151
BfuAI	ACCTGC	2 cut(s)	634, 755
BisI	GCNGC	8 cut(s)	127, 130, 133, 591, 594, 762, 1062, 1065
BlsI	GCNGC	8 cut(s)	128, 131, 134, 592, 595, 763, 1063, 1066
Bme1390I	CCNGG	4 cut(s)	52, 110, 886, 899
BmeRI	GACNNNNNGTC	1 cut(s)	68
BmeT110I	CYCGRG	1 cut(s)	1092
BmgBI	CACGTC	1 cut(s)	448
BmiI	GGNNCC	1 cut(s)	265
BmrFI	CCNGG	4 cut(s)	52, 110, 886, 899
BmsI	GCATC	5 cut(s)	242, 345, 364, 462, 511
BpiI	GAAGAC	1 cut(s)	974
BpuMI	CCSGG	1 cut(s)	52
BsaAI	YACGTR	1 cut(s)	90
BsaHI	GRCGYC	1 cut(s)	246
BsaI	GGTCTC	1 cut(s)	32
BsaJI	CCNNGG	3 cut(s)	20, 267, 702
BsaXI	ACNNNNNCTCC	4 cut(s)	200, 230, 380, 410
Bsc4I	CCNNNNNNNGG	3 cut(s)	727, 927, 1087
Bse118I	RCCGGY	1 cut(s)	122
Bse1I	ACTGG	2 cut(s)	788, 921
Bse21I	CCTNAGG	1 cut(s)	845
BseBI	CCWGG	3 cut(s)	110, 886, 899
BseDI	CCNNGG	3 cut(s)	20, 267, 702
BseGI	GGATG	7 cut(s)	360, 477, 574, 745, 907, 912, 943
BseLI	CCNNNNNNNGG	3 cut(s)	727, 927, 1087
BseMII	CTCAG	3 cut(s)	79, 836, 1047
BseNI	ACTGG	2 cut(s)	788, 921
BseRI	GAGGAG	1 cut(s)	653
BseSI	GKGCMC	1 cut(s)	435
BseXI	GCAGC	4 cut(s)	141, 602, 748, 1073
BseYI	CCCAGC	2 cut(s)	452, 1142
BsgI	GTGCAG	1 cut(s)	134
BshFI	GGCC	7 cut(s)	19, 135, 272, 656, 707, 850, 990
BsiHKCI	CYCGRG	1 cut(s)	1092
BsiSI	CCGG	4 cut(s)	52, 123, 273, 728
BslFI	GGGAC	1 cut(s)	523
BslI	CCNNNNNNNGG	3 cut(s)	727, 927, 1087
BsmAI	GTCTC	3 cut(s)	32, 346, 530
BsmBI	CGTCTC	2 cut(s)	346, 530
BsmFI	GGGAC	1 cut(s)	523
BsmI	GAATGC	1 cut(s)	52
BsnI	GGCC	7 cut(s)	19, 135, 272, 656, 707, 850, 990
Bso31I	GGTCTC	1 cut(s)	32
BsoBI	CYCGRG	1 cut(s)	1092
Bsp1286I	GDGCHC	1 cut(s)	435
Bsp143I	GATC	5 cut(s)	105, 117, 263, 275, 824
BspACI	CCGC	8 cut(s)	126, 132, 288, 386, 488, 593, 617, 1064
BspANI	GGCC	7 cut(s)	19, 135, 272, 656, 707, 850, 990
BspCNI	CTCAG	3 cut(s)	78, 837, 1048
BspLI	GGNNCC	1 cut(s)	265
BspMAI	CTGCAG	1 cut(s)	766
BspMI	ACCTGC	2 cut(s)	634, 755
BspPI	GGATC	4 cut(s)	100, 258, 271, 283
BspTNI	GGTCTC	1 cut(s)	32
BsrBI	CCGCTC	1 cut(s)	386
BsrFI	RCCGGY	1 cut(s)	122
BsrI	ACTGG	2 cut(s)	788, 921
BssAI	RCCGGY	1 cut(s)	122
BssECI	CCNNGG	3 cut(s)	20, 267, 702
BssMI	GATC	5 cut(s)	105, 117, 263, 275, 824
BssNI	GRCGYC	1 cut(s)	246
BssT1I	CCWWGG	1 cut(s)	702
Bst2UI	CCWGG	3 cut(s)	110, 886, 899
Bst4CI	ACNGT	4 cut(s)	437, 541, 604, 880
Bst6I	CTCTTC	3 cut(s)	312, 743, 992
BstACI	GRCGYC	1 cut(s)	246
BstBAI	YACGTR	1 cut(s)	90
BstC8I	GCNNGC	2 cut(s)	813, 1148
BstDEI	CTNAG	3 cut(s)	65, 845, 1056
BstDSI	CCRYGG	2 cut(s)	20, 267
BstEII	GGTNACC	2 cut(s)	833, 880
BstF5I	GGATG	7 cut(s)	360, 477, 574, 745, 907, 912, 943
BstKTI	GATC	5 cut(s)	108, 120, 266, 278, 827
BstMAI	GTCTC	3 cut(s)	32, 346, 530
BstMBI	GATC	5 cut(s)	105, 117, 263, 275, 824
BstMWI	GCNNNNNNNGC	4 cut(s)	132, 383, 449, 623
BstNI	CCWGG	3 cut(s)	110, 886, 899
BstNSI	RCATGY	1 cut(s)	963
BstPI	GGTNACC	2 cut(s)	833, 880
BstSCI	CCNGG	4 cut(s)	50, 108, 884, 897
BstSFI	CTRYAG	2 cut(s)	762, 1151
BstSLI	GKGCMC	1 cut(s)	435
BstV1I	GCAGC	4 cut(s)	141, 602, 748, 1073
BstV2I	GAAGAC	1 cut(s)	974
BstX2I	RGATCY	2 cut(s)	105, 263
BstYI	RGATCY	2 cut(s)	105, 263
Bsu36I	CCTNAGG	1 cut(s)	845
BsuRI	GGCC	7 cut(s)	19, 135, 272, 656, 707, 850, 990
BtgI	CCRYGG	2 cut(s)	20, 267
BtrI	CACGTC	1 cut(s)	448
BtsCI	GGATG	7 cut(s)	360, 477, 574, 745, 907, 912, 943
BtsI	GCAGTG	1 cut(s)	384
BtsIMutI	CAGTG	2 cut(s)	384, 928
BveI	ACCTGC	2 cut(s)	634, 755
Cac8I	GCNNGC	2 cut(s)	813, 1148
Cfr10I	RCCGGY	1 cut(s)	122
Csp6I	GTAC	2 cut(s)	85, 800
CviAII	CATG	3 cut(s)	143, 476, 960
CviQI	GTAC	2 cut(s)	85, 800
DdeI	CTNAG	3 cut(s)	65, 845, 1056
DpnI	GATC	5 cut(s)	107, 119, 265, 277, 826
DpnII	GATC	5 cut(s)	105, 117, 263, 275, 824
DriI	GACNNNNNGTC	1 cut(s)	68
EaeI	YGGCCR	4 cut(s)	133, 270, 654, 988
Eam1104I	CTCTTC	3 cut(s)	312, 743, 992
Eam1105I	GACNNNNNGTC	1 cut(s)	68
EarI	CTCTTC	3 cut(s)	312, 743, 992
EciI	GGCGGA	2 cut(s)	303, 503
Eco130I	CCWWGG	1 cut(s)	702
Eco31I	GGTCTC	1 cut(s)	32
Eco32I	GATATC	1 cut(s)	861
Eco57I	CTGAAG	1 cut(s)	101
Eco72I	CACGTG	1 cut(s)	90
Eco81I	CCTNAGG	1 cut(s)	845
Eco88I	CYCGRG	1 cut(s)	1092
Eco91I	GGTNACC	2 cut(s)	833, 880
EcoO65I	GGTNACC	2 cut(s)	833, 880
EcoRI	GAATTC	1 cut(s)	905
EcoRII	CCWGG	3 cut(s)	108, 884, 897
EcoRV	GATATC	1 cut(s)	861
EcoT14I	CCWWGG	1 cut(s)	702
EcoT22I	ATGCAT	1 cut(s)	477
ErhI	CCWWGG	1 cut(s)	702
Esp3I	CGTCTC	2 cut(s)	346, 530
FaeI	CATG	3 cut(s)	146, 479, 963
FalI	AAGNNNNNCTT	2 cut(s)	395, 427
FaqI	GGGAC	1 cut(s)	523
FatI	CATG	3 cut(s)	142, 475, 959
FauNDI	CATATG	1 cut(s)	853
FblI	GTMKAC	1 cut(s)	1128
Fnu4HI	GCNGC	8 cut(s)	127, 130, 133, 591, 594, 762, 1062, 1065
FokI	GGATG	7 cut(s)	367, 484, 581, 752, 899, 914, 950
Fsp4HI	GCNGC	8 cut(s)	127, 130, 133, 591, 594, 762, 1062, 1065
FspBI	CTAG	3 cut(s)	258, 672, 1082
GluI	GCNGC	8 cut(s)	127, 130, 133, 591, 594, 762, 1062, 1065
GsaI	CCCAGC	2 cut(s)	456, 1146
HaeIII	GGCC	7 cut(s)	19, 135, 272, 656, 707, 850, 990
HapII	CCGG	4 cut(s)	52, 123, 273, 728
Hin1I	GRCGYC	1 cut(s)	246
Hin1II	CATG	3 cut(s)	146, 479, 963
HincII	GTYRAC	2 cut(s)	1078, 1129
HindII	GTYRAC	2 cut(s)	1078, 1129
HinfI	GANTC	5 cut(s)	63, 69, 146, 668, 774
HpaII	CCGG	4 cut(s)	52, 123, 273, 728
Hpy166II	GTNNAC	4 cut(s)	87, 800, 1078, 1129
Hpy188I	TCNGA	7 cut(s)	68, 74, 151, 280, 316, 409, 1057
Hpy188III	TCNNGA	5 cut(s)	37, 687, 822, 864, 1013
Hpy8I	GTNNAC	4 cut(s)	87, 800, 1078, 1129
Hpy99I	CGWCG	4 cut(s)	78, 251, 956, 1019
HpyAV	CCTTC	5 cut(s)	169, 278, 343, 355, 500
HpyCH4III	ACNGT	4 cut(s)	437, 541, 604, 880
HpyCH4IV	ACGT	4 cut(s)	89, 246, 447, 954
HpyCH4V	TGCA	6 cut(s)	115, 159, 377, 475, 590, 764
HpyF10VI	GCNNNNNNNGC	4 cut(s)	132, 383, 449, 623
HpyF3I	CTNAG	3 cut(s)	65, 845, 1056
HpySE526I	ACGT	4 cut(s)	89, 246, 447, 954
Hsp92I	GRCGYC	1 cut(s)	246
Hsp92II	CATG	3 cut(s)	146, 479, 963
Kzo9I	GATC	5 cut(s)	105, 117, 263, 275, 824
LmnI	GCTCC	1 cut(s)	640
Lsp1109I	GCAGC	4 cut(s)	141, 602, 748, 1073
LweI	GCATC	5 cut(s)	242, 345, 364, 462, 511
MaeI	CTAG	3 cut(s)	258, 672, 1082
MaeII	ACGT	4 cut(s)	89, 246, 447, 954
MaeIII	GTNAC	4 cut(s)	833, 874, 880, 923
MalI	GATC	5 cut(s)	107, 119, 265, 277, 826
MbiI	CCGCTC	1 cut(s)	386
MboI	GATC	5 cut(s)	105, 117, 263, 275, 824
MboII	GAAGA	8 cut(s)	112, 115, 164, 329, 383, 760, 974, 1009
MfeI	CAATTG	1 cut(s)	658
MflI	RGATCY	2 cut(s)	105, 263
MhlI	GDGCHC	1 cut(s)	435
MlsI	TGGCCA	1 cut(s)	656
MluCI	AATT	3 cut(s)	658, 753, 905
MluNI	TGGCCA	1 cut(s)	656
MlyI	GAGTC	3 cut(s)	57, 78, 783
MmeI	TCCRAC	1 cut(s)	97
Mox20I	TGGCCA	1 cut(s)	656
Mph1103I	ATGCAT	1 cut(s)	477
MscI	TGGCCA	1 cut(s)	656
MseI	TTAA	1 cut(s)	972
MslI	CAYNNNNRTG	1 cut(s)	1115
Msp20I	TGGCCA	1 cut(s)	656
MspA1I	CMGCKG	4 cut(s)	132, 593, 617, 1064
MspI	CCGG	4 cut(s)	52, 123, 273, 728
MspR9I	CCNGG	4 cut(s)	52, 110, 886, 899
MunI	CAATTG	1 cut(s)	658
Mva1269I	GAATGC	1 cut(s)	52
MvaI	CCWGG	3 cut(s)	110, 886, 899
MwoI	GCNNNNNNNGC	4 cut(s)	132, 383, 449, 623
NciI	CCSGG	1 cut(s)	52
NdeI	CATATG	1 cut(s)	853
NdeII	GATC	5 cut(s)	105, 117, 263, 275, 824
NlaIII	CATG	3 cut(s)	146, 479, 963
NlaIV	GGNNCC	1 cut(s)	265
NmuCI	GTSAC	4 cut(s)	833, 874, 880, 923
NsiI	ATGCAT	1 cut(s)	477
NspI	RCATGY	1 cut(s)	963
PaqCI	CACCTGC	2 cut(s)	634, 755
PcsI	WCGNNNNNNNCGW	2 cut(s)	95, 608
PctI	GAATGC	1 cut(s)	52
PfeI	GAWTC	2 cut(s)	146, 668
PkrI	GCNGC	8 cut(s)	128, 131, 134, 592, 595, 763, 1063, 1066
PleI	GAGTC	3 cut(s)	57, 77, 782
PmaCI	CACGTG	1 cut(s)	90
PmlI	CACGTG	1 cut(s)	90
PpsI	GAGTC	3 cut(s)	57, 77, 782
Ppu21I	YACGTR	1 cut(s)	90
Psp6I	CCWGG	3 cut(s)	108, 884, 897
PspCI	CACGTG	1 cut(s)	90
PspEI	GGTNACC	2 cut(s)	833, 880
PspFI	CCCAGC	2 cut(s)	452, 1142
PspGI	CCWGG	3 cut(s)	108, 884, 897
PspN4I	GGNNCC	1 cut(s)	265
PstI	CTGCAG	1 cut(s)	766
PsuI	RGATCY	2 cut(s)	105, 263
RsaI	GTAC	2 cut(s)	86, 801
RsaNI	GTAC	2 cut(s)	85, 800
RseI	CAYNNNNRTG	1 cut(s)	1115
SalI	GTCGAC	1 cut(s)	1127
SaqAI	TTAA	1 cut(s)	972
SatI	GCNGC	8 cut(s)	127, 130, 133, 591, 594, 762, 1062, 1065
Sau3AI	GATC	5 cut(s)	105, 117, 263, 275, 824
SchI	GAGTC	3 cut(s)	57, 78, 783
ScrFI	CCNGG	4 cut(s)	52, 110, 886, 899
SduI	GDGCHC	1 cut(s)	435
SfaNI	GCATC	5 cut(s)	242, 345, 364, 462, 511
SfcI	CTRYAG	2 cut(s)	762, 1151
SgrAI	CRCCGGYG	1 cut(s)	122
SmiMI	CAYNNNNRTG	1 cut(s)	1115
Sse9I	AATT	3 cut(s)	658, 753, 905
SsiI	CCGC	8 cut(s)	126, 132, 288, 386, 488, 593, 617, 1064
SspMI	CTAG	3 cut(s)	258, 672, 1082
StyD4I	CCNGG	4 cut(s)	50, 108, 884, 897
StyI	CCWWGG	1 cut(s)	702
TaaI	ACNGT	4 cut(s)	437, 541, 604, 880
TaiI	ACGT	4 cut(s)	92, 249, 450, 957
TaqI	TCGA	4 cut(s)	36, 236, 686, 1128
TasI	AATT	3 cut(s)	658, 753, 905
TatI	WGTACW	2 cut(s)	84, 799
TauI	GCSGC	4 cut(s)	129, 135, 596, 1067
TfiI	GAWTC	2 cut(s)	146, 668
Tru1I	TTAA	1 cut(s)	972
Tru9I	TTAA	1 cut(s)	972
TscAI	CASTG	2 cut(s)	384, 928
TseFI	GTSAC	4 cut(s)	833, 874, 880, 923
TseI	GCWGC	4 cut(s)	129, 590, 761, 1061
Tsp45I	GTSAC	4 cut(s)	833, 874, 880, 923
TspDTI	ATGAA	4 cut(s)	17, 297, 918, 1010
TspGWI	ACGGA	3 cut(s)	37, 256, 804
TspRI	CASTG	2 cut(s)	384, 928
XapI	RAATTY	1 cut(s)	905
XceI	RCATGY	1 cut(s)	963
XmiI	GTMKAC	1 cut(s)	1128
XspI	CTAG	3 cut(s)	258, 672, 1082
ZraI	GACGTC	1 cut(s)	247
Zsp2I	ATGCAT	1 cut(s)	477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.