Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0013722

Physical Location & Seq

Genomic Coordinates Reverse (-)

52673395 .. 52680396

Gene Structure 7002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0426700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3537 bp

ATGTCGAGCGATCCGAATCCAAACCCACCGCCTCTGACCTCGACCTCCGCCGCCGGCAACGCAGACCCCGCCGCCGCATCGTCTCCGACTCATCCTTCTCCCTCCAGAGCCAAGCTCCGTAAGATTCCTCCCATTCCGATTCACCGGAGCCCTAGGCATTTGGCCGAGGTCAACGAACAAAGCGACGAAGAGGGAGACGATGATGAAGATGACGACGACAACGATGATTTTAGGGAACGTAGAGAGACCACGCCGATCCTATTGGCTGCGTCGTTAGGGCTCAATCACATACGGACTCGATCGGCGCCGTCACCGCTCCGGTTCTCTTCCTCCGCCGGCGAGTCTCTAATTCCCGGCGAGGAAGCGAATAATATGAACTACACGGTCAAGTCAAAGCCCCAACCAGTGAAATATGTGCACTCGAGTCAATCAAAATCGCTGAGAACTCCATCGCCACTCAATCCTGTGTTGGAGGGTGGTCAAGCTGGTCTTACAAAGGAAATGCAGTCGCCTAGGTTTCAGGCAATACTGCGAGTTACGAGTGGACGAAAGAAGAGAACACCTGACATCAAGAGTTTCTCTCATGAACTAAATTCCAAAGGAGTTAGACCATTTCCGGTGTGGAAGTCCCGTGCATTCGGGCATATGGAGGAGGTTTTAGTGGCGCTCAGGGCAAGATTTGAGAGGTTGAAGGATGAGGTTGATGTTGATTTGGGTGCATTTGCCGGTGATTTGGTGGGTATACTTGAAATGTCTTCAGAGTCTCACCCTGAATGGAAAGAGAATTTTGAGGATTTGCTAGTTATTGCCCGGCAGTGTGCAAAGATGTCTCCTAACGAATTTTGGATCAAGTGTGAAGGCATTGTACAGAAGTTGGATGACCGGCGTCAAGAGCTACCAATGGGGGCCCTGAAACAAGCCCATACTCGTCTTCTTTTTATTCTTACTCGGTGCACACGGCTTGTGCAGTTCCAGAAAGAGAGTGGTTATGAGGAAGAGCACATTCTTAGTCTTCACCAGCTCAGTGACCTTGGTGTTTATCCAGAACAAATTTTGGACGGTGCAGAACAGAAGTTTAGTGGTCCACTGAGTGGGAAAGATGCAAACGAGAAGCCCAAGAAGTCTCGTGAGCAAGAAATAGCTAGTGGAGCTGATGGTGTGGAGGTTAGCACTGCCAAGAGCGTTGAATCTACTGGTAGCTATAGGATGTCATCTTGGAAAAAACTTCCATCGGCTGCTGAGAAAAAGCAGAAAGTACCTGATTCTGTTGATACACCATCGAAGGACAAATCAGAAAGTTTGCATGCCAAAGAAAATCTGGATACTCCATCATCCCATCATGAACACTCAAAGATGTCCACAAGTGCACAAAGAGTATCTTGGGTTTCAATTGGGGAGCAGCAAAATGTTACATATGAGAATTTGATGATCTGTCGGATATGTGAGGTTGAAATACCAACTGTCCACGTAGAGGAGCACTCTCGAATATGTACAATTGCTGACAGATGTGACTTGAAGGGCTTAACTGTGAATGAAAGACTTGAAAGAGTTGCTGAAGCTCTTGAAAGGATAATGGAATCTCGGACAACAAAAAATGTTGATACACAAGAGACAAGTTTTGATGCTGCAAAAGTGTATCCATCAAGTATCCATGAAGATTTTATTGCCAAAAGAAAAAAAAGTATCCATGAAGATTTAGATGACTTATCACCTAAACGAAATGAATTGCCTCGTTTCCCTGAAGACATGCTTGATTGTGTTCCTGATGCTGACAATTCTTTTGTCATGGAAGATATTAATATTATGCCTGACATGTCATGTGACGCACGTTCTGCTTTGACACCTGAACAAGGAACCAGAACCTCATCAGCGGGAAGCTCCACTCCAAGATCGCCACTTTTAACACCTAGAACCACTCATATTGAAATGCTGTTGAGTGGAAGGGGAACAATACCAGAACTTGAGAATTGTCAGCAGATACACAAGCTACTGGATATTGCTCGTTCTGTTGCAAATGTTAGCAATTGTGACTATAGTGCATTGGAGTATATGCTTGACCGACTAGAAGACCTAAAATATGCTATCCAGGACAGGAAGGTGGATGCCCTTGTTGTAGAGACGTTTGGAAGACGTATTGAAAAATTATTGCAGGAAAAATATGTACATCTTTGTGGGCAGATAGAAGATGAGAAATTGGATTCATCAAATGGTATGGCAGACGAAGAAAGTTCAGTGGAAGATGATACAGTTCGTAGTCTACGTACCAGCCCCATCAATCCGTCTTCTAAGGATCGAACATCTATTGAAGATTTTGAAATAATAAAACCAATAAGCAGGGGTGCATTTGGACGAGTTTTTCTTGCCAGAAAACGAGCGACTGGTGACTTATTTGCTATAAAGGTATTAAAGAAGGCTGATATGATTCGTAAGAATGCAGTTGAAAGTATTTTGGCAGAGCGTAACATCCTTATTTTGGTCCGCAATCCTTTTGTGGTCCGATTTTTCTATTCCTTCACATGCAGGGAAAATCTTTATCTGGTCATGGAGTACTTAAATGGTGGAGATCTTTACTCTTTATTGAGAAATCTAGGCTGCTTAGATGAAGATATGGCCCGTGTATATATTGCAGAAGTTGTTCTTGCTTTGGAGTATCTGCATTCATTAAATGTTATTCATAGAGACTTGAAGCCAGACAACTTGTTGATTGGTCAAGATGGTCACATCAAGTTGACAGATTTTGGGCTCTCCAAGGTTGGTCTAATCAGCAGCACTGATGACTTAGCAGGTCCATCTGTTGGTGATACTGGTTTTGTTGGAGACGATGAACCAAAAAGTCAATCTTCATCTCAGAGGTCACAGCGTCAAAAACATTCAGTTGTTGGCACCCCCGATTATTTGGCACCTGAAATACTTCTTGGCATGGGACATAGTGCAACTGCTGATTGGTGGTCTGTGGGTGTTATACTTTTTGAGCTACTTGTTGGCATTCCACCATTCAATGCAGAACATCCACAGCAAATTTTCAACAATATAATAAACAGAGATATCCCCTGGCCTAAGGTACCTGAGGAGATGAGCAACGAAGCATATGACTTAATTGACCAATTACTGACTGAAAACCCAGTTCAAAGACTTGGAGCAACAGGTGCTAGGGAGGTGAAGCAACATACTTTTTTCAAGGATATTAACTGGGATACACTTGCAAGGCAGAAGGCTATGTTCATACCTTCAACAGATGTACATGATACAAGTTATTTCATGAGCCGGTACATATGGAATCCAGAAGATGAGCATGTTAATGGAGGCAGTGACTTCGATGACCTGACTGAAACATGCAGCAGTACTGGTTCATATTATCTAGATGAAGACGGGGATGAGTGTGGTAGTTTGGCGGACTTCAGTGCTCCGGCCCTTGATGTGCAGTACTCATTTAGTAATTTCTCATTTAAGAACTTGTCTCAGCTGGCTTCCATCAATTATGATCTTGTCGTGAAGACCAAGGAGTCACCAGAAGCCTCCAAGTCATCTGTTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010030 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:0140096 GO:1900140 GO:1901419 GO:1901420 GO:1901564 GO:1905957 GO:1905958 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

1178

Amino Acids

131.73

Weight (kDa)

5.2

Isoelectric Point (pI)

50.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IREH1	PF26031	208 - 326	2.8e-55	IREH1-like domain
Pkinase	PF00069	771 - 1059	4.9e-65	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	772 - 918	1.1e-24	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	950 - 1045	4.4e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013722)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g31810
malus_domestica	MD01G1220800.v1.1 MD07G1291000.v1.1
prunus_persica	Prupe.2G312300_v2.0.a1
pyrus_communis	pycom01g23030 pycom07g26360
rosa_chinensis	RchiOBHm_Chr1g0381041
rosa_laevigata	RLG00000026250
rosa_multiflora	Rmu_co8392071.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278830
rosa_rugosa	Rorug01G0426700 Rorug01G0426800 Rorug01G0426900
rosa_samantha	Rh1AG450500 Rh1BG406800 Rh1CG420600 Rh1DG437300
rosa_wichuraiana	Rw1G039260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3502
Acc36I	ACCTGC	1 cut(s)	2774
Acc65I	GGTACC	1 cut(s)	3061
AccB1I	GGYRCC	4 cut(s)	304, 2882, 2899, 3061
AccB7I	CCANNNNNTGG	2 cut(s)	1091, 2795
AccBSI	CCGCTC	1 cut(s)	316
AccI	GTMKAC	2 cut(s)	742, 2257
AclWI	GGATC	4 cut(s)	5, 250, 854, 2298
AcoI	YGGCCR	1 cut(s)	162
AcsI	RAATTY	6 cut(s)	592, 784, 839, 1050, 1420, 3018
AcuI	CTGAAG	4 cut(s)	741, 1575, 1761, 3382
AcyI	GRCGYC	2 cut(s)	305, 886
AdeI	CACNNNGTG	1 cut(s)	1091
AfiI	CCNNNNNNNGG	5 cut(s)	1091, 1471, 1850, 2473, 2795
AflIII	ACRYGT	1 cut(s)	1812
AjnI	CCWGG	2 cut(s)	2085, 3050
AjuI	GAANNNNNNNTTGG	2 cut(s)	2898, 2930
Alw21I	GWGCWC	6 cut(s)	420, 956, 1002, 1369, 1479, 3406
Alw44I	GTGCAC	3 cut(s)	416, 952, 1365
AlwI	GGATC	4 cut(s)	5, 250, 854, 2298
Ama87I	CYCGRG	1 cut(s)	421
AoxI	GGCC	5 cut(s)	162, 906, 2610, 3053, 3408
ApaI	GGGCCC	1 cut(s)	910
ApaLI	GTGCAC	3 cut(s)	416, 952, 1365
ApeKI	GCWGC	7 cut(s)	266, 1235, 1399, 1625, 2592, 2766, 3336
ApoI	RAATTY	6 cut(s)	592, 784, 839, 1050, 1420, 3018
ArsI	GACNNNNNNTTYG	2 cut(s)	1763, 1795
AseI	ATTAAT	1 cut(s)	1797
Asp700I	GAANNNNTTC	2 cut(s)	2634, 2910
Asp718I	GGTACC	1 cut(s)	3061
AspA2I	CCTAGG	2 cut(s)	152, 512
AspLEI	GCGC	2 cut(s)	307, 667
AspS9I	GGNCC	8 cut(s)	906, 907, 1082, 2476, 2494, 2611, 2786, 3409
AsuC2I	CCSGG	2 cut(s)	354, 811
AvaI	CYCGRG	1 cut(s)	421
AvaII	GGWCC	4 cut(s)	1082, 2476, 2494, 2786
AvrII	CCTAGG	2 cut(s)	152, 512
AxyI	CCTNAGG	2 cut(s)	3057, 3066
BaeGI	GKGCMC	4 cut(s)	420, 910, 956, 1369
BanI	GGYRCC	4 cut(s)	304, 2882, 2899, 3061
BanII	GRGCYC	4 cut(s)	152, 282, 910, 2745
BarI	GAAGNNNNNNTAC	2 cut(s)	849, 881
BauI	CACGAG	1 cut(s)	1125
BbsI	GAAGAC	9 cut(s)	747, 923, 1004, 1749, 2073, 2134, 2274, 3372, 3500
Bbv12I	GWGCWC	6 cut(s)	420, 956, 1002, 1369, 1479, 3406
BbvI	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2579, 2778, 3348
BceAI	ACGGC	2 cut(s)	292, 974
BcgI	CGANNNNNNTGC	2 cut(s)	3295, 3329
BciT130I	CCWGG	2 cut(s)	2087, 3052
BciVI	GTATCC	5 cut(s)	1315, 1647, 1658, 1694, 3187
BcnI	CCSGG	2 cut(s)	354, 811
BfaI	CTAG	9 cut(s)	153, 513, 800, 1143, 1908, 2063, 2588, 3150, 3359
BfmI	CTRYAG	2 cut(s)	1201, 2032
BfoI	RGCGCY	2 cut(s)	308, 668
BfuAI	ACCTGC	1 cut(s)	2774
BfuI	GTATCC	5 cut(s)	1315, 1647, 1658, 1694, 3187
BglII	AGATCT	1 cut(s)	2563
BlnI	CCTAGG	2 cut(s)	152, 512
BmcAI	AGTACT	3 cut(s)	2549, 3343, 3425
Bme1390I	CCNGG	4 cut(s)	354, 811, 2087, 3052
Bme18I	GGWCC	4 cut(s)	1082, 2476, 2494, 2786
BmeT110I	CYCGRG	1 cut(s)	421
BmgT120I	GGNCC	8 cut(s)	906, 907, 1082, 2476, 2494, 2611, 2786, 3409
BmiI	GGNNCC	8 cut(s)	149, 306, 907, 908, 1855, 2884, 2901, 3063
BmrFI	CCNGG	4 cut(s)	354, 811, 2087, 3052
BmrI	ACTGGG	2 cut(s)	3116, 3199
BmsI	GCATC	5 cut(s)	86, 1090, 1612, 1756, 2092
BmuI	ACTGGG	2 cut(s)	3116, 3199
BoxI	GACNNNNGTC	1 cut(s)	885
BpiI	GAAGAC	9 cut(s)	747, 923, 1004, 1749, 2073, 2134, 2274, 3372, 3500
BplI	GAGNNNNNCTC	6 cut(s)	99, 131, 565, 597, 1463, 1495
BpmI	CTGGAG	1 cut(s)	88
Bpu10I	CCTNAGC	1 cut(s)	668
BpuEI	CTTGAG	1 cut(s)	1982
BpuMI	CCSGG	2 cut(s)	354, 811
BsaAI	YACGTR	2 cut(s)	1468, 2261
BsaBI	GATNNNNATC	1 cut(s)	15
BsaHI	GRCGYC	2 cut(s)	305, 886
BsaI	GGTCTC	1 cut(s)	239
BsaJI	CCNNGG	7 cut(s)	152, 165, 512, 1030, 2748, 3050, 3498
BsaWI	WCCGGW	3 cut(s)	144, 318, 616
Bsc4I	CCNNNNNNNGG	5 cut(s)	1091, 1471, 1850, 2473, 2795
Bse118I	RCCGGY	5 cut(s)	53, 335, 725, 882, 3264
Bse1I	ACTGG	8 cut(s)	404, 1198, 1995, 2383, 2809, 3122, 3194, 3349
Bse21I	CCTNAGG	2 cut(s)	3057, 3066
Bse8I	GATNNNNATC	1 cut(s)	15
BseBI	CCWGG	2 cut(s)	2087, 3052
BseDI	CCNNGG	7 cut(s)	152, 165, 512, 1030, 2748, 3050, 3498
BseGI	GGATG	9 cut(s)	91, 700, 883, 1212, 1331, 2107, 2463, 3007, 3379
BseJI	GATNNNNATC	1 cut(s)	15
BseLI	CCNNNNNNNGG	5 cut(s)	1091, 1471, 1850, 2473, 2795
BseMII	CTCAG	8 cut(s)	431, 682, 1036, 1079, 1230, 2861, 3057, 3473
BseNI	ACTGG	8 cut(s)	404, 1198, 1995, 2383, 2809, 3122, 3194, 3349
BseRI	GAGGAG	3 cut(s)	665, 1487, 3083
BseSI	GKGCMC	4 cut(s)	420, 910, 956, 1369
BseXI	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2579, 2778, 3348
BsgI	GTGCAG	3 cut(s)	986, 1083, 3440
Bsh1285I	CGRYCG	1 cut(s)	302
BshFI	GGCC	5 cut(s)	164, 908, 2612, 3055, 3410
BshNI	GGYRCC	4 cut(s)	304, 2882, 2899, 3061
BsiEI	CGRYCG	1 cut(s)	302
BsiHKAI	GWGCWC	6 cut(s)	420, 956, 1002, 1369, 1479, 3406
BsiHKCI	CYCGRG	1 cut(s)	421
BslFI	GGGAC	2 cut(s)	613, 2937
BslI	CCNNNNNNNGG	5 cut(s)	1091, 1471, 1850, 2473, 2795
BsmBI	CGTCTC	4 cut(s)	87, 189, 2111, 2811
BsmFI	GGGAC	2 cut(s)	613, 2937
BsmI	GAATGC	4 cut(s)	635, 2437, 2656, 2985
BsnI	GGCC	5 cut(s)	164, 908, 2612, 3055, 3410
Bso31I	GGTCTC	1 cut(s)	239
BsoBI	CYCGRG	1 cut(s)	421
Bsp120I	GGGCCC	1 cut(s)	906
Bsp1407I	TGTACA	4 cut(s)	865, 1490, 2161, 3238
Bsp143I	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
BspANI	GGCC	5 cut(s)	164, 908, 2612, 3055, 3410
BspCNI	CTCAG	8 cut(s)	432, 681, 1035, 1080, 1231, 2860, 3058, 3472
BspHI	TCATGA	3 cut(s)	583, 1339, 3258
BspLI	GGNNCC	8 cut(s)	149, 306, 907, 908, 1855, 2884, 2901, 3063
BspMI	ACCTGC	1 cut(s)	2774
BspPI	GGATC	4 cut(s)	5, 250, 854, 2298
BspQI	GCTCTTC	1 cut(s)	990
BspT107I	GGYRCC	4 cut(s)	304, 2882, 2899, 3061
BspTNI	GGTCTC	1 cut(s)	239
BsrBI	CCGCTC	1 cut(s)	316
BsrFI	RCCGGY	5 cut(s)	53, 335, 725, 882, 3264
BsrGI	TGTACA	4 cut(s)	865, 1490, 2161, 3238
BsrI	ACTGG	8 cut(s)	404, 1198, 1995, 2383, 2809, 3122, 3194, 3349
BssAI	RCCGGY	5 cut(s)	53, 335, 725, 882, 3264
BssECI	CCNNGG	7 cut(s)	152, 165, 512, 1030, 2748, 3050, 3498
BssMI	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
BssNAI	GTATAC	1 cut(s)	743
BssNI	GRCGYC	2 cut(s)	305, 886
BssSI	CACGAG	1 cut(s)	1125
BssT1I	CCWWGG	5 cut(s)	152, 512, 1030, 2748, 3498
Bst1107I	GTATAC	1 cut(s)	743
Bst2BI	CACGAG	1 cut(s)	1125
Bst2UI	CCWGG	2 cut(s)	2087, 3052
Bst4CI	ACNGT	5 cut(s)	385, 1061, 1462, 1528, 2248
Bst6I	CTCTTC	4 cut(s)	183, 331, 548, 990
BstACI	GRCGYC	2 cut(s)	305, 886
BstAPI	GCANNNNNTGC	2 cut(s)	1832, 3146
BstAUI	TGTACA	4 cut(s)	865, 1490, 2161, 3238
BstBAI	YACGTR	2 cut(s)	1468, 2261
BstC8I	GCNNGC	4 cut(s)	55, 337, 1305, 3465
BstENI	CCTNNNNNAGG	1 cut(s)	1848
BstF5I	GGATG	9 cut(s)	91, 700, 883, 1212, 1331, 2107, 2463, 3007, 3379
BstH2I	RGCGCY	2 cut(s)	308, 668
BstHHI	GCGC	2 cut(s)	307, 667
BstKTI	GATC	9 cut(s)	13, 258, 302, 849, 1431, 1892, 2293, 2566, 3484
BstMBI	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
BstMCI	CGRYCG	1 cut(s)	302
BstMWI	GCNNNNNNNGC	8 cut(s)	59, 68, 313, 671, 892, 1148, 1832, 3146
BstNI	CCWGG	2 cut(s)	2087, 3052
BstNSI	RCATGY	6 cut(s)	1307, 1750, 1816, 2520, 3296, 3336
BstPAI	GACNNNNGTC	1 cut(s)	885
BstSCI	CCNGG	4 cut(s)	352, 809, 2085, 3050
BstSFI	CTRYAG	2 cut(s)	1201, 2032
BstSLI	GKGCMC	4 cut(s)	420, 910, 956, 1369
BstSNI	TACGTA	1 cut(s)	2261
BstV1I	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2579, 2778, 3348
BstV2I	GAAGAC	9 cut(s)	747, 923, 1004, 1749, 2073, 2134, 2274, 3372, 3500
BstX2I	RGATCY	1 cut(s)	2563
BstYI	RGATCY	1 cut(s)	2563
BstZ17I	GTATAC	1 cut(s)	743
Bsu36I	CCTNAGG	2 cut(s)	3057, 3066
BsuI	GTATCC	5 cut(s)	1315, 1647, 1658, 1694, 3187
BsuRI	GGCC	5 cut(s)	164, 908, 2612, 3055, 3410
BtgZI	GCGATG	1 cut(s)	435
BtsCI	GGATG	9 cut(s)	91, 700, 883, 1212, 1331, 2107, 2463, 3007, 3379
BtsI	GCAGTG	3 cut(s)	821, 1170, 3313
BtsIMutI	CAGTG	9 cut(s)	411, 821, 1030, 1085, 1170, 2238, 2769, 3313, 3406
BveI	ACCTGC	1 cut(s)	2774
Cac8I	GCNNGC	4 cut(s)	55, 337, 1305, 3465
CciI	TCATGA	3 cut(s)	583, 1339, 3258
CfoI	GCGC	2 cut(s)	307, 667
Cfr10I	RCCGGY	5 cut(s)	53, 335, 725, 882, 3264
Cfr13I	GGNCC	8 cut(s)	906, 907, 1082, 2476, 2494, 2611, 2786, 3409
CseI	GACGC	4 cut(s)	258, 875, 1832, 2849
CspCI	CAANNNNNGTGG	2 cut(s)	1903, 1938
DinI	GGCGCC	1 cut(s)	306
DpnI	GATC	9 cut(s)	12, 257, 301, 848, 1430, 1891, 2292, 2565, 3483
DpnII	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
DraIII	CACNNNGTG	1 cut(s)	1091
DrdI	GACNNNNNNGTC	1 cut(s)	3502
DseDI	GACNNNNNNGTC	1 cut(s)	3502
EaeI	YGGCCR	1 cut(s)	162
Eam1104I	CTCTTC	4 cut(s)	183, 331, 548, 990
EarI	CTCTTC	4 cut(s)	183, 331, 548, 990
EciI	GGCGGA	3 cut(s)	37, 322, 3407
Eco105I	TACGTA	1 cut(s)	2261
Eco130I	CCWWGG	5 cut(s)	152, 512, 1030, 2748, 3498
Eco24I	GRGCYC	4 cut(s)	152, 282, 910, 2745
Eco31I	GGTCTC	1 cut(s)	239
Eco32I	GATATC	1 cut(s)	3046
Eco47I	GGWCC	4 cut(s)	1082, 2476, 2494, 2786
Eco57I	CTGAAG	4 cut(s)	741, 1575, 1761, 3382
Eco81I	CCTNAGG	2 cut(s)	3057, 3066
Eco88I	CYCGRG	1 cut(s)	421
EcoNI	CCTNNNNNAGG	1 cut(s)	1848
EcoO109I	RGGNCCY	2 cut(s)	906, 907
EcoRII	CCWGG	2 cut(s)	2085, 3050
EcoRV	GATATC	1 cut(s)	3046
EcoT14I	CCWWGG	5 cut(s)	152, 512, 1030, 2748, 3498
EcoT38I	GRGCYC	4 cut(s)	152, 282, 910, 2745
EgeI	GGCGCC	1 cut(s)	306
EheI	GGCGCC	1 cut(s)	306
ErhI	CCWWGG	5 cut(s)	152, 512, 1030, 2748, 3498
Esp3I	CGTCTC	4 cut(s)	87, 189, 2111, 2811
FaqI	GGGAC	2 cut(s)	613, 2937
FauI	CCCGC	2 cut(s)	76, 1864
FauNDI	CATATG	4 cut(s)	645, 1414, 3088, 3272
FblI	GTMKAC	2 cut(s)	742, 2257
FokI	GGATG	9 cut(s)	78, 707, 890, 1219, 1318, 2114, 2450, 2994, 3386
FriOI	GRGCYC	4 cut(s)	152, 282, 910, 2745
FspBI	CTAG	9 cut(s)	153, 513, 800, 1143, 1908, 2063, 2588, 3150, 3359
GlaI	GCGC	2 cut(s)	306, 666
GsuI	CTGGAG	1 cut(s)	88
HaeII	RGCGCY	2 cut(s)	308, 668
HaeIII	GGCC	5 cut(s)	164, 908, 2612, 3055, 3410
HgaI	GACGC	4 cut(s)	258, 875, 1832, 2849
HhaI	GCGC	2 cut(s)	307, 667
Hin1I	GRCGYC	2 cut(s)	305, 886
Hin6I	GCGC	2 cut(s)	305, 665
HinP1I	GCGC	2 cut(s)	305, 665
HincII	GTYRAC	2 cut(s)	172, 2730
HindII	GTYRAC	2 cut(s)	172, 2730
Hpy99I	CGWCG	3 cut(s)	188, 218, 274
HpyCH4III	ACNGT	5 cut(s)	385, 1061, 1462, 1528, 2248
HpyCH4IV	ACGT	6 cut(s)	238, 1467, 1828, 2120, 2131, 2260
HpyF10VI	GCNNNNNNNGC	8 cut(s)	59, 68, 313, 671, 892, 1148, 1832, 3146
HpySE526I	ACGT	6 cut(s)	238, 1467, 1828, 2120, 2131, 2260
Hsp92I	GRCGYC	2 cut(s)	305, 886
HspAI	GCGC	2 cut(s)	305, 665
KasI	GGCGCC	1 cut(s)	304
KpnI	GGTACC	1 cut(s)	3065
KroI	GCCGGC	2 cut(s)	53, 335
KroNI	GCCGGC	2 cut(s)	55, 337
Kzo9I	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
LguI	GCTCTTC	1 cut(s)	990
LmnI	GCTCC	9 cut(s)	120, 147, 321, 1148, 1396, 1474, 1883, 3137, 3409
Lsp1109I	GCAGC	7 cut(s)	253, 1222, 1411, 1612, 2579, 2778, 3348
LweI	GCATC	5 cut(s)	86, 1090, 1612, 1756, 2092
MaeI	CTAG	9 cut(s)	153, 513, 800, 1143, 1908, 2063, 2588, 3150, 3359
MaeII	ACGT	6 cut(s)	238, 1467, 1828, 2120, 2131, 2260
MalI	GATC	9 cut(s)	12, 257, 301, 848, 1430, 1891, 2292, 2565, 3483
MbiI	CCGCTC	1 cut(s)	316
MboI	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
MfeI	CAATTG	3 cut(s)	1389, 1494, 2023
MflI	RGATCY	1 cut(s)	2563
Mly113I	GGCGCC	1 cut(s)	305
MlyI	GAGTC	6 cut(s)	82, 289, 350, 433, 770, 3512
MmeI	TCCRAC	5 cut(s)	110, 450, 855, 1415, 2794
MreI	CGCCGGCG	1 cut(s)	335
MroNI	GCCGGC	2 cut(s)	53, 335
MroXI	GAANNNNTTC	2 cut(s)	2634, 2910
MslI	CAYNNNNRTG	2 cut(s)	2169, 3297
MspA1I	CMGCKG	2 cut(s)	1871, 3463
MspR9I	CCNGG	4 cut(s)	354, 811, 2087, 3052
MunI	CAATTG	3 cut(s)	1389, 1494, 2023
Mva1269I	GAATGC	4 cut(s)	635, 2437, 2656, 2985
MvaI	CCWGG	2 cut(s)	2087, 3052
MwoI	GCNNNNNNNGC	8 cut(s)	59, 68, 313, 671, 892, 1148, 1832, 3146
NaeI	GCCGGC	2 cut(s)	55, 337
NarI	GGCGCC	1 cut(s)	305
NciI	CCSGG	2 cut(s)	354, 811
NdeI	CATATG	4 cut(s)	645, 1414, 3088, 3272
NdeII	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
NgoMIV	GCCGGC	2 cut(s)	53, 335
NlaIV	GGNNCC	8 cut(s)	149, 306, 907, 908, 1855, 2884, 2901, 3063
NmeAIII	GCCGAG	1 cut(s)	190
NspI	RCATGY	6 cut(s)	1307, 1750, 1816, 2520, 3296, 3336
PaeI	GCATGC	1 cut(s)	1307
PaeR7I	CTCGAG	1 cut(s)	421
PagI	TCATGA	3 cut(s)	583, 1339, 3258
PciI	ACATGT	1 cut(s)	1812
PciSI	GCTCTTC	1 cut(s)	990
PcsI	WCGNNNNNNNCGW	4 cut(s)	11, 180, 219, 2257
PctI	GAATGC	4 cut(s)	635, 2437, 2656, 2985
PdiI	GCCGGC	2 cut(s)	55, 337
PdmI	GAANNNNTTC	2 cut(s)	2634, 2910
PfeI	GAWTC	9 cut(s)	16, 124, 139, 1187, 1262, 1577, 2198, 2422, 3277
PflMI	CCANNNNNTGG	2 cut(s)	1091, 2795
PfoI	TCCNGGA	1 cut(s)	2085
Ple19I	CGATCG	1 cut(s)	302
PleI	GAGTC	6 cut(s)	82, 289, 349, 432, 769, 3511
PluTI	GGCGCC	1 cut(s)	308
PpsI	GAGTC	6 cut(s)	82, 289, 349, 432, 769, 3511
Ppu21I	YACGTR	2 cut(s)	1468, 2261
PscI	ACATGT	1 cut(s)	1812
PshAI	GACNNNNGTC	1 cut(s)	885
PshBI	ATTAAT	1 cut(s)	1797
Psp6I	CCWGG	2 cut(s)	2085, 3050
PspGI	CCWGG	2 cut(s)	2085, 3050
PspN4I	GGNNCC	8 cut(s)	149, 306, 907, 908, 1855, 2884, 2901, 3063
PspOMI	GGGCCC	1 cut(s)	906
PspPI	GGNCC	8 cut(s)	906, 907, 1082, 2476, 2494, 2611, 2786, 3409
PspXI	VCTCGAGB	1 cut(s)	421
PsuI	RGATCY	1 cut(s)	2563
PvuI	CGATCG	1 cut(s)	302
PvuII	CAGCTG	1 cut(s)	3463
RseI	CAYNNNNRTG	2 cut(s)	2169, 3297
SapI	GCTCTTC	1 cut(s)	990
Sau3AI	GATC	9 cut(s)	10, 255, 299, 846, 1428, 1889, 2290, 2563, 3481
Sau96I	GGNCC	8 cut(s)	906, 907, 1082, 2476, 2494, 2611, 2786, 3409
ScaI	AGTACT	3 cut(s)	2549, 3343, 3425
SchI	GAGTC	6 cut(s)	82, 289, 350, 433, 770, 3512
ScrFI	CCNGG	4 cut(s)	354, 811, 2087, 3052
SfaNI	GCATC	5 cut(s)	86, 1090, 1612, 1756, 2092
SfcI	CTRYAG	2 cut(s)	1201, 2032
SfoI	GGCGCC	1 cut(s)	306
Sfr274I	CTCGAG	1 cut(s)	421
SgrAI	CRCCGGYG	1 cut(s)	335
SinI	GGWCC	4 cut(s)	1082, 2476, 2494, 2786
SlaI	CTCGAG	1 cut(s)	421
SmiMI	CAYNNNNRTG	2 cut(s)	2169, 3297
SmlI	CTYRAG	2 cut(s)	421, 1961
SmoI	CTYRAG	2 cut(s)	421, 1961
SnaBI	TACGTA	1 cut(s)	2261
SphI	GCATGC	1 cut(s)	1307
SspDI	GGCGCC	1 cut(s)	304
SspI	AATATT	1 cut(s)	1801
SspMI	CTAG	9 cut(s)	153, 513, 800, 1143, 1908, 2063, 2588, 3150, 3359
StyD4I	CCNGG	4 cut(s)	352, 809, 2085, 3050
StyI	CCWWGG	5 cut(s)	152, 512, 1030, 2748, 3498
TaaI	ACNGT	5 cut(s)	385, 1061, 1462, 1528, 2248
TaiI	ACGT	6 cut(s)	241, 1470, 1831, 2123, 2134, 2263
TaqI	TCGA	8 cut(s)	5, 41, 298, 422, 1280, 1483, 2293, 3315
TaqII	GACCGA	1 cut(s)	2073
TatI	WGTACW	7 cut(s)	865, 1490, 2161, 2547, 3238, 3341, 3423
TauI	GCSGC	3 cut(s)	53, 74, 77
TfiI	GAWTC	9 cut(s)	16, 124, 139, 1187, 1262, 1577, 2198, 2422, 3277
TscAI	CASTG	9 cut(s)	411, 821, 1030, 1092, 1177, 2238, 2776, 3313, 3406
TseI	GCWGC	7 cut(s)	266, 1235, 1399, 1625, 2592, 2766, 3336
TspGWI	ACGGA	3 cut(s)	107, 307, 2268
TspRI	CASTG	9 cut(s)	411, 821, 1030, 1092, 1177, 2238, 2776, 3313, 3406
Van91I	CCANNNNNTGG	2 cut(s)	1091, 2795
VneI	GTGCAC	3 cut(s)	416, 952, 1365
VpaK11BI	GGWCC	4 cut(s)	1082, 2476, 2494, 2786
VspI	ATTAAT	1 cut(s)	1797
XagI	CCTNNNNNAGG	1 cut(s)	1848
XapI	RAATTY	6 cut(s)	592, 784, 839, 1050, 1420, 3018
XbaI	TCTAGA	1 cut(s)	3358
XceI	RCATGY	6 cut(s)	1307, 1750, 1816, 2520, 3296, 3336
XcmI	CCANNNNNNNNNTGG	3 cut(s)	618, 980, 1315
XhoI	CTCGAG	1 cut(s)	421
XmaJI	CCTAGG	2 cut(s)	152, 512
XmiI	GTMKAC	2 cut(s)	742, 2257
XmnI	GAANNNNTTC	2 cut(s)	2634, 2910
XspI	CTAG	9 cut(s)	153, 513, 800, 1143, 1908, 2063, 2588, 3150, 3359
ZrmI	AGTACT	3 cut(s)	2549, 3343, 3425

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.