Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009148

Physical Location & Seq

Genomic Coordinates Reverse (-)

31493342 .. 31497954

Gene Structure 4613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1222900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGGACAGGTCTAGATCTAAACGGTATTACTATGAACAGGACTACGATACGGAGACTGTGGGTAGGACGAGGCCTCGGTACAACCACAACTATGCGAACAACAACAACCACCGCAACCGCGGAAGTGGCGGTGGCGGTGGCGGCGGTGGTGGAAGACCCAATAAGCCGCCTCAGCAGGACCCGTCGGTTACGGTAACTACGACGTACCGTATTCTCTGTCACGATATGAAGGCTGGAGGGGTGATTGGGAAGTCCGGTAGCATCATTAAGTCGATCCGGCAGCACACCGGCGCATGGATCAACGTGCATGAACTGATTCCCGGAGACGAGGAGCGGATTATTGAGATTTCCGATACGCGGCGGCGGGACCCGGAGGGGCGAATGCCGTCGTTCTCGCCGGCCCAGGAGGCGCTATTTTTGGTCCATGAGAGGATTCTGGAGAGTGATGGTGCTGGGTTTGGCGGTGAGGAGGATGATGAGTACGGTGGCGGGGTTAGAGGAAGTGGCGGCGGCGGCGGTGGTGGTGGGAATCGCGTGGCGACGCGGCTGGTCGTGTCGAGAATGCATGTGGGGTGTTTGCTTGGGAAGGGTGGGAAGATAATTGAGCAAATGAGGATGGAGACAAAGACCCAGATTAGGGTGTTGCCCAGGGATCAAAATCTGCCCCGCTGTGTTTCGATGTCGGAGGAGATTGTTCAGGTAGTAGGCGATCCGAATAATGTAAAGAGTGCTGTGGCAATTATTTCATCACGCTTGAGGGAGAGTCAGCATCGCGACCGCAGTCATTTCCAGGGGCGAATGCATTCACCCGAACGGTTCTTTCCTCCTGAAGATGATTATATTCCTCATATGAACAATGCAGGACGGAAGGCACTCATGGATGGGGCTCCCTTTGGACCGCGATTATCTAACCCCAATATCAGAAACAACAACTATGCCCCCCGTTCATCTGGTTATCCAATTGAACCTGGGTCTGCTCATATGACTGATAATGTGCAGCCCTTTTCTGAAGACCTTGTGTTTCGAATACTCTGTCCAGTTGACAAGGTTGATCTTATAGTTGGAGATTCTGCCGGAATTATGGAATTGCTTCAAAATGAAATTGGTGTAGATGTCAAGGCTACAAACCCTGTGGTGGGTGAAGATGAACAGATAATCATAATCTCTTCCGAGGAGGGTCCTGATGACGAGCTGTTTCCAGCTCAGGAAGCTTTGTTGCATATTCAAACTCGCATTGTTGATCTTAGTCCAGACAAAGAGAACATTATAACTACCAGGTTACTTGTCCCAACAAGTGATATTGGCTGGTTAGAGGGAAGAGATGGGTTGTTATTGGAAATGAGGCGACTTACTGGTGCAGATATACAAATTCTTCCCATAGATGATCTTCCCGCATGTGTATCAGGGGACGATGTGCTTATACAGATTGTAGGGGAGATAAAAGCAGCTCGAGAAGCTCTTGTTGAGGTGACGTCAAGAATACGGAGTTACTTATATAGAGAGTCGTTTCGAAAGGATACAACACCACCTGCTTTTGCACCAGGCCCTGGGGGCAGTGCTTTCGCAATGGAGCCATCTTCGAATAACACATTTCCAATTCGTGAAGCTCATGCTGGAAATGATCCTCCGATGAAAATGTATCGGAATGTGCAAACCGTGGGAATAGCACAGCCATCAAAGGATGCTGGAGGGATTGCAAGTGAAACCGTAAAGCCAAACGAAAGTGAACGTCGTGAAGATGTTCCTAGTTCTTTGAATAGAATACCTGTGACACTTGTCACTAGGAGTATACTAGAAGTCGTGATACCAGAGCAGGCAGTTTCAAAGCTCATAACGAAATCGAGAAACAAGCTTGCTCAGATCAGCGAGTTATCGGGAGCCAGTGTGACCCTGGTAGAGGATAGACCGGAGGAAACACAAAAGATCATTCGAATTTCAGGAACTCCAGAGCAGGCTGAGAGAGCTCAGAGCTTGCTTCAGGGCTTTATTTTAAGCACACAAGAAGACGGGCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002831 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010286 GO:0010445 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0019222 GO:0023052 GO:0031047 GO:0031050 GO:0031053 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032870 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043900 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070878 GO:0070887 GO:0070918 GO:0071229 GO:0071310 GO:0071359 GO:0071395 GO:0071407 GO:0071495 GO:0071704 GO:0080134 GO:0090304 GO:0097159 GO:1900150 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

672

Amino Acids

73.52

Weight (kDa)

5.57

Isoelectric Point (pI)

56.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	68 - 117	2.2e-10	KH domain
KH_1	PF00013	182 - 249	2.9e-12	KH domain
KH_1	PF00013	598 - 660	1.1e-09	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53060 AT5G53060 AT5G53060
fragaria_vesca	FvH4_7g32010 FvH4_7g32010
malus_domestica	MD01G1222900.v1.1 MD07G1293700.v1.1
prunus_persica	Prupe.2G313500_v2.0.a1
pyrus_communis	pycom01g23230 pycom07g26650
rosa_chinensis	RchiOBHm_Chr1g0381621
rosa_laevigata	RLG00000026220
rosa_multiflora	Rmu_sc0003688.1_g000012 Rmu_sc0005891.1_g000007
rosa_roxburghii	Rroxscaffold_4G00278580
rosa_rugosa	Rorug01G0429700
rosa_samantha	Rh1AG453000 Rh1BG410000 Rh1CG424600 Rh1DG441800 Rh2CG399100
rosa_wichuraiana	Rw1G039520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1268
AarI	CACCTGC	1 cut(s)	1537
AatII	GACGTC	1 cut(s)	1475
Acc36I	ACCTGC	1 cut(s)	1537
AccB7I	CCANNNNNTGG	1 cut(s)	1547
AccBSI	CCGCTC	1 cut(s)	334
AccI	GTMKAC	1 cut(s)	1789
AccII	CGCG	6 cut(s)	120, 358, 534, 544, 774, 901
AclWI	GGATC	5 cut(s)	268, 305, 660, 704, 1616
AcsI	RAATTY	2 cut(s)	1368, 1932
AcuI	CTGAAG	3 cut(s)	849, 1029, 1961
AcyI	GRCGYC	1 cut(s)	1472
AfaI	GTAC	3 cut(s)	80, 206, 482
AfiI	CCNNNNNNNGG	8 cut(s)	294, 357, 636, 637, 1135, 1136, 1547, 1897
AgsI	TTSAA	5 cut(s)	965, 1094, 1226, 1756, 1824
AjnI	CCWGG	8 cut(s)	402, 647, 789, 967, 1274, 1540, 1546, 1890
Alw21I	GWGCWC	1 cut(s)	1966
Alw26I	GTCTC	3 cut(s)	47, 318, 614
AlwI	GGATC	5 cut(s)	268, 305, 660, 704, 1616
AlwNI	CAGNNNCTG	1 cut(s)	1547
Ama87I	CYCGRG	1 cut(s)	1449
AoxI	GGCC	4 cut(s)	71, 399, 1543, 2009
ApeKI	GCWGC	3 cut(s)	280, 997, 1445
ApoI	RAATTY	2 cut(s)	1368, 1932
ArsI	GACNNNNNNTTYG	2 cut(s)	1018, 1050
Asp700I	GAANNNNTTC	4 cut(s)	315, 802, 1089, 1740
AspLEI	GCGC	2 cut(s)	293, 412
AspS9I	GGNCC	8 cut(s)	178, 367, 400, 421, 896, 1178, 1544, 2009
AsuC2I	CCSGG	2 cut(s)	321, 371
AsuHPI	GGTGA	5 cut(s)	253, 476, 798, 1151, 1480
AsuII	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
AvaI	CYCGRG	1 cut(s)	1449
AvaII	GGWCC	5 cut(s)	178, 367, 421, 896, 1178
BanII	GRGCYC	2 cut(s)	889, 1966
BarI	GAAGNNNNNNTAC	2 cut(s)	1788, 1820
BbsI	GAAGAC	3 cut(s)	160, 1017, 2010
Bbv12I	GWGCWC	1 cut(s)	1966
BbvCI	CCTCAGC	1 cut(s)	171
BbvI	GCAGC	3 cut(s)	292, 1009, 1457
BccI	CCATC	6 cut(s)	440, 610, 875, 1316, 1582, 1681
BceAI	ACGGC	1 cut(s)	370
BcgI	CGANNNNNNTGC	2 cut(s)	1543, 1577
BciT130I	CCWGG	8 cut(s)	404, 649, 791, 969, 1276, 1542, 1548, 1892
BciVI	GTATCC	1 cut(s)	1510
BcnI	CCSGG	2 cut(s)	321, 371
BcoDI	GTCTC	3 cut(s)	47, 318, 614
BfaI	CTAG	4 cut(s)	12, 1746, 1782, 1793
BfoI	RGCGCY	1 cut(s)	413
BfuAI	ACCTGC	1 cut(s)	1537
BfuI	GTATCC	1 cut(s)	1510
BglI	GCCNNNNNGGC	2 cut(s)	407, 1551
BglII	AGATCT	1 cut(s)	14
Bme18I	GGWCC	5 cut(s)	178, 367, 421, 896, 1178
BmeT110I	CYCGRG	1 cut(s)	1449
BmgT120I	GGNCC	8 cut(s)	178, 367, 400, 421, 896, 1178, 1544, 2009
BmiI	GGNNCC	7 cut(s)	180, 368, 369, 888, 1179, 1572, 1879
BmsI	GCATC	3 cut(s)	270, 778, 1672
BoxI	GACNNNNGTC	2 cut(s)	780, 1775
BpiI	GAAGAC	3 cut(s)	160, 1017, 2010
BpmI	CTGGAG	4 cut(s)	255, 458, 1707, 1929
Bpu10I	CCTNAGC	2 cut(s)	171, 1203
Bpu14I	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
BpuEI	CTTGAG	1 cut(s)	775
BpuMI	CCSGG	2 cut(s)	321, 371
BsaBI	GATNNNNATC	1 cut(s)	657
BsaHI	GRCGYC	1 cut(s)	1472
BsaWI	WCCGGW	2 cut(s)	254, 1906
BsaXI	ACNNNNNCTCC	2 cut(s)	1869, 1899
Bsc4I	CCNNNNNNNGG	8 cut(s)	294, 357, 636, 637, 1135, 1136, 1547, 1897
Bse118I	RCCGGY	2 cut(s)	287, 397
Bse1I	ACTGG	3 cut(s)	1037, 1357, 1881
Bse3DI	GCAATG	1 cut(s)	1572
Bse8I	GATNNNNATC	1 cut(s)	657
BseBI	CCWGG	8 cut(s)	404, 649, 791, 969, 1276, 1542, 1548, 1892
BseGI	GGATG	4 cut(s)	478, 621, 886, 1687
BseJI	GATNNNNATC	1 cut(s)	657
BseLI	CCNNNNNNNGG	8 cut(s)	294, 357, 636, 637, 1135, 1136, 1547, 1897
BseMI	GCAATG	1 cut(s)	1572
BseMII	CTCAG	5 cut(s)	185, 1217, 1871, 1947, 1979
BseNI	ACTGG	3 cut(s)	1037, 1357, 1881
BseRI	GAGGAG	4 cut(s)	344, 482, 701, 1187
BseXI	GCAGC	3 cut(s)	292, 1009, 1457
BseYI	CCCAGC	1 cut(s)	452
BsgI	GTGCAG	2 cut(s)	1016, 1377
Bsh1236I	CGCG	6 cut(s)	120, 358, 534, 544, 774, 901
Bsh1285I	CGRYCG	1 cut(s)	778
BshFI	GGCC	4 cut(s)	73, 401, 1545, 2011
BsiEI	CGRYCG	1 cut(s)	778
BsiHKAI	GWGCWC	1 cut(s)	1966
BsiHKCI	CYCGRG	1 cut(s)	1449
BsiSI	CCGG	8 cut(s)	255, 277, 288, 321, 371, 398, 1074, 1907
BslFI	GGGAC	3 cut(s)	380, 1271, 1421
BslI	CCNNNNNNNGG	8 cut(s)	294, 357, 636, 637, 1135, 1136, 1547, 1897
BsmAI	GTCTC	3 cut(s)	47, 318, 614
BsmBI	CGTCTC	1 cut(s)	318
BsmFI	GGGAC	3 cut(s)	380, 1271, 1421
BsmI	GAATGC	4 cut(s)	387, 567, 802, 804
BsnI	GGCC	4 cut(s)	73, 401, 1545, 2011
BsoBI	CYCGRG	1 cut(s)	1449
Bsp119I	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
Bsp1286I	GDGCHC	2 cut(s)	889, 1966
Bsp68I	TCGCGA	1 cut(s)	774
BspANI	GGCC	4 cut(s)	73, 401, 1545, 2011
BspCNI	CTCAG	5 cut(s)	184, 1216, 1870, 1948, 1978
BspFNI	CGCG	6 cut(s)	120, 358, 534, 544, 774, 901
BspLI	GGNNCC	7 cut(s)	180, 368, 369, 888, 1179, 1572, 1879
BspMI	ACCTGC	1 cut(s)	1537
BspPI	GGATC	5 cut(s)	268, 305, 660, 704, 1616
BspT104I	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
BsrBI	CCGCTC	1 cut(s)	334
BsrDI	GCAATG	1 cut(s)	1572
BsrFI	RCCGGY	2 cut(s)	287, 397
BsrI	ACTGG	3 cut(s)	1037, 1357, 1881
BssAI	RCCGGY	2 cut(s)	287, 397
BssNAI	GTATAC	1 cut(s)	1790
BssNI	GRCGYC	1 cut(s)	1472
Bst1107I	GTATAC	1 cut(s)	1790
Bst2UI	CCWGG	8 cut(s)	404, 649, 791, 969, 1276, 1542, 1548, 1892
Bst4CI	ACNGT	8 cut(s)	24, 58, 193, 209, 485, 816, 1657, 1708
Bst6I	CTCTTC	2 cut(s)	1171, 1312
BstACI	GRCGYC	1 cut(s)	1472
BstBI	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
BstC8I	GCNNGC	5 cut(s)	399, 1815, 1854, 1953, 1973
BstDEI	CTNAG	7 cut(s)	171, 1203, 1244, 1857, 1956, 1965, 2013
BstDSI	CCRYGG	2 cut(s)	118, 1656
BstENI	CCTNNNNNAGG	1 cut(s)	1895
BstF5I	GGATG	4 cut(s)	478, 621, 886, 1687
BstFNI	CGCG	6 cut(s)	120, 358, 534, 544, 774, 901
BstH2I	RGCGCY	1 cut(s)	413
BstHHI	GCGC	2 cut(s)	293, 412
BstMAI	GTCTC	3 cut(s)	47, 318, 614
BstMCI	CGRYCG	1 cut(s)	778
BstMWI	GCNNNNNNNGC	9 cut(s)	126, 141, 172, 407, 513, 1208, 1454, 1551, 1961
BstNI	CCWGG	8 cut(s)	404, 649, 791, 969, 1276, 1542, 1548, 1892
BstNSI	RCATGY	2 cut(s)	569, 1398
BstPAI	GACNNNNGTC	2 cut(s)	780, 1775
BstUI	CGCG	6 cut(s)	120, 358, 534, 544, 774, 901
BstV1I	GCAGC	3 cut(s)	292, 1009, 1457
BstV2I	GAAGAC	3 cut(s)	160, 1017, 2010
BstX2I	RGATCY	1 cut(s)	14
BstYI	RGATCY	1 cut(s)	14
BstZ17I	GTATAC	1 cut(s)	1790
BsuI	GTATCC	1 cut(s)	1510
BsuRI	GGCC	4 cut(s)	73, 401, 1545, 2011
BtgI	CCRYGG	2 cut(s)	118, 1656
BtgZI	GCGATG	1 cut(s)	755
BtsCI	GGATG	4 cut(s)	478, 621, 886, 1687
BtsI	GCAGTG	1 cut(s)	1561
BtsIMutI	CAGTG	2 cut(s)	1561, 1888
BtuMI	TCGCGA	1 cut(s)	774
BveI	ACCTGC	1 cut(s)	1537
Cac8I	GCNNGC	5 cut(s)	399, 1815, 1854, 1953, 1973
CaiI	CAGNNNCTG	1 cut(s)	1547
CfoI	GCGC	2 cut(s)	293, 412
Cfr10I	RCCGGY	2 cut(s)	287, 397
Cfr13I	GGNCC	8 cut(s)	178, 367, 400, 421, 896, 1178, 1544, 2009
Cfr42I	CCGCGG	1 cut(s)	121
CseI	GACGC	1 cut(s)	550
CsiI	ACCWGGT	1 cut(s)	1274
Csp6I	GTAC	3 cut(s)	79, 205, 481
CspCI	CAANNNNNGTGG	2 cut(s)	1113, 1148
CviAII	CATG	7 cut(s)	294, 308, 425, 566, 877, 1395, 1610
CviQI	GTAC	3 cut(s)	79, 205, 481
DdeI	CTNAG	7 cut(s)	171, 1203, 1244, 1857, 1956, 1965, 2013
Eam1104I	CTCTTC	2 cut(s)	1171, 1312
EarI	CTCTTC	2 cut(s)	1171, 1312
Ecl136II	GAGCTC	1 cut(s)	1964
Eco147I	AGGCCT	1 cut(s)	73
Eco24I	GRGCYC	2 cut(s)	889, 1966
Eco47I	GGWCC	5 cut(s)	178, 367, 421, 896, 1178
Eco53kI	GAGCTC	1 cut(s)	1964
Eco57I	CTGAAG	3 cut(s)	849, 1029, 1961
Eco88I	CYCGRG	1 cut(s)	1449
EcoICRI	GAGCTC	1 cut(s)	1964
EcoNI	CCTNNNNNAGG	1 cut(s)	1895
EcoO109I	RGGNCCY	4 cut(s)	178, 367, 1178, 1544
EcoRII	CCWGG	8 cut(s)	402, 647, 789, 967, 1274, 1540, 1546, 1890
EcoT22I	ATGCAT	2 cut(s)	567, 804
EcoT38I	GRGCYC	2 cut(s)	889, 1966
Esp3I	CGTCTC	1 cut(s)	318
FaeI	CATG	7 cut(s)	297, 311, 428, 569, 880, 1398, 1613
FaqI	GGGAC	3 cut(s)	380, 1271, 1421
FatI	CATG	7 cut(s)	293, 307, 424, 565, 876, 1394, 1609
FauI	CCCGC	4 cut(s)	357, 482, 674, 1399
FauNDI	CATATG	2 cut(s)	849, 981
FblI	GTMKAC	1 cut(s)	1789
FokI	GGATG	4 cut(s)	485, 628, 893, 1694
FriOI	GRGCYC	2 cut(s)	889, 1966
FspBI	CTAG	4 cut(s)	12, 1746, 1782, 1793
GlaI	GCGC	2 cut(s)	292, 411
GsaI	CCCAGC	1 cut(s)	456
GsuI	CTGGAG	4 cut(s)	255, 458, 1707, 1929
HaeII	RGCGCY	1 cut(s)	413
HaeIII	GGCC	4 cut(s)	73, 401, 1545, 2011
HapII	CCGG	8 cut(s)	255, 277, 288, 321, 371, 398, 1074, 1907
HgaI	GACGC	1 cut(s)	550
HhaI	GCGC	2 cut(s)	293, 412
Hin1I	GRCGYC	1 cut(s)	1472
Hin1II	CATG	7 cut(s)	297, 311, 428, 569, 880, 1398, 1613
Hin6I	GCGC	2 cut(s)	291, 410
HinP1I	GCGC	2 cut(s)	291, 410
HincII	GTYRAC	1 cut(s)	1042
HindII	GTYRAC	1 cut(s)	1042
HindIII	AAGCTT	2 cut(s)	1209, 1850
HinfI	GANTC	6 cut(s)	316, 433, 529, 763, 1067, 1502
HpaII	CCGG	8 cut(s)	255, 277, 288, 321, 371, 398, 1074, 1907
HphI	GGTGA	5 cut(s)	253, 476, 798, 1151, 1480
Hpy166II	GTNNAC	3 cut(s)	1042, 1727, 1790
Hpy8I	GTNNAC	3 cut(s)	1042, 1727, 1790
Hpy99I	CGWCG	5 cut(s)	187, 205, 391, 544, 1734
HpyAV	CCTTC	3 cut(s)	223, 580, 862
HpyCH4III	ACNGT	8 cut(s)	24, 58, 193, 209, 485, 816, 1657, 1708
HpyCH4IV	ACGT	4 cut(s)	203, 303, 1472, 1729
HpyF10VI	GCNNNNNNNGC	9 cut(s)	126, 141, 172, 407, 513, 1208, 1454, 1551, 1961
HpyF3I	CTNAG	7 cut(s)	171, 1203, 1244, 1857, 1956, 1965, 2013
HpySE526I	ACGT	4 cut(s)	203, 303, 1472, 1729
Hsp92I	GRCGYC	1 cut(s)	1472
Hsp92II	CATG	7 cut(s)	297, 311, 428, 569, 880, 1398, 1613
HspAI	GCGC	2 cut(s)	291, 410
KflI	GGGWCCC	1 cut(s)	367
KroI	GCCGGC	1 cut(s)	397
KroNI	GCCGGC	1 cut(s)	399
KspI	CCGCGG	1 cut(s)	121
LmnI	GCTCC	4 cut(s)	331, 892, 1570, 1877
Lsp1109I	GCAGC	3 cut(s)	292, 1009, 1457
LweI	GCATC	3 cut(s)	270, 778, 1672
MabI	ACCWGGT	1 cut(s)	1274
MaeI	CTAG	4 cut(s)	12, 1746, 1782, 1793
MaeII	ACGT	4 cut(s)	203, 303, 1472, 1729
MaeIII	GTNAC	9 cut(s)	187, 193, 218, 1278, 1468, 1487, 1768, 1777, 1885
MbiI	CCGCTC	1 cut(s)	334
MfeI	CAATTG	1 cut(s)	960
MflI	RGATCY	1 cut(s)	14
MhlI	GDGCHC	2 cut(s)	889, 1966
MluCI	AATT	9 cut(s)	600, 738, 960, 1077, 1085, 1101, 1368, 1596, 1932
MlyI	GAGTC	2 cut(s)	772, 1511
MmeI	TCCRAC	2 cut(s)	663, 1042
Mph1103I	ATGCAT	2 cut(s)	567, 804
MroNI	GCCGGC	1 cut(s)	397
MroXI	GAANNNNTTC	4 cut(s)	315, 802, 1089, 1740
MseI	TTAA	2 cut(s)	267, 1991
MslI	CAYNNNNRTG	1 cut(s)	90
MspA1I	CMGCKG	2 cut(s)	120, 669
MspI	CCGG	8 cut(s)	255, 277, 288, 321, 371, 398, 1074, 1907
MunI	CAATTG	1 cut(s)	960
Mva1269I	GAATGC	4 cut(s)	387, 567, 802, 804
MvaI	CCWGG	8 cut(s)	404, 649, 791, 969, 1276, 1542, 1548, 1892
MvnI	CGCG	6 cut(s)	120, 358, 534, 544, 774, 901
MwoI	GCNNNNNNNGC	9 cut(s)	126, 141, 172, 407, 513, 1208, 1454, 1551, 1961
NaeI	GCCGGC	1 cut(s)	399
NciI	CCSGG	2 cut(s)	321, 371
NdeI	CATATG	2 cut(s)	849, 981
NgoMIV	GCCGGC	1 cut(s)	397
NlaIII	CATG	7 cut(s)	297, 311, 428, 569, 880, 1398, 1613
NlaIV	GGNNCC	7 cut(s)	180, 368, 369, 888, 1179, 1572, 1879
NmuCI	GTSAC	5 cut(s)	218, 1468, 1768, 1777, 1885
NruI	TCGCGA	1 cut(s)	774
NsiI	ATGCAT	2 cut(s)	567, 804
NspI	RCATGY	2 cut(s)	569, 1398
NspV	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
PaeR7I	CTCGAG	1 cut(s)	1449
PaqCI	CACCTGC	1 cut(s)	1537
PasI	CCCWGGG	2 cut(s)	648, 1547
PceI	AGGCCT	1 cut(s)	73
PcsI	WCGNNNNNNNCGW	1 cut(s)	197
PctI	GAATGC	4 cut(s)	387, 567, 802, 804
PdiI	GCCGGC	1 cut(s)	399
PdmI	GAANNNNTTC	4 cut(s)	315, 802, 1089, 1740
PfeI	GAWTC	4 cut(s)	316, 433, 529, 1067
PflMI	CCANNNNNTGG	1 cut(s)	1547
PfoI	TCCNGGA	1 cut(s)	319
PleI	GAGTC	2 cut(s)	771, 1510
PpsI	GAGTC	2 cut(s)	771, 1510
PpuMI	RGGWCCY	3 cut(s)	178, 367, 1178
PshAI	GACNNNNGTC	2 cut(s)	780, 1775
PsiI	TTATAA	1 cut(s)	1268
Psp124BI	GAGCTC	1 cut(s)	1966
Psp5II	RGGWCCY	3 cut(s)	178, 367, 1178
Psp6I	CCWGG	8 cut(s)	402, 647, 789, 967, 1274, 1540, 1546, 1890
PspFI	CCCAGC	1 cut(s)	452
PspGI	CCWGG	8 cut(s)	402, 647, 789, 967, 1274, 1540, 1546, 1890
PspN4I	GGNNCC	7 cut(s)	180, 368, 369, 888, 1179, 1572, 1879
PspPI	GGNCC	8 cut(s)	178, 367, 400, 421, 896, 1178, 1544, 2009
PspPPI	RGGWCCY	3 cut(s)	178, 367, 1178
PstNI	CAGNNNCTG	1 cut(s)	1547
PsuI	RGATCY	1 cut(s)	14
RruI	TCGCGA	1 cut(s)	774
RsaI	GTAC	3 cut(s)	80, 206, 482
RsaNI	GTAC	3 cut(s)	79, 205, 481
RseI	CAYNNNNRTG	1 cut(s)	90
SacI	GAGCTC	1 cut(s)	1966
SacII	CCGCGG	1 cut(s)	121
SaqAI	TTAA	2 cut(s)	267, 1991
Sau96I	GGNCC	8 cut(s)	178, 367, 400, 421, 896, 1178, 1544, 2009
SchI	GAGTC	2 cut(s)	772, 1511
SduI	GDGCHC	2 cut(s)	889, 1966
SexAI	ACCWGGT	1 cut(s)	1274
SfaNI	GCATC	3 cut(s)	270, 778, 1672
Sfr274I	CTCGAG	1 cut(s)	1449
Sfr303I	CCGCGG	1 cut(s)	121
SfuI	TTCGAA	4 cut(s)	1024, 1510, 1580, 1930
SgrAI	CRCCGGYG	1 cut(s)	287
SgrBI	CCGCGG	1 cut(s)	121
SinI	GGWCC	5 cut(s)	178, 367, 421, 896, 1178
SlaI	CTCGAG	1 cut(s)	1449
SmiMI	CAYNNNNRTG	1 cut(s)	90
SmlI	CTYRAG	2 cut(s)	754, 1449
SmoI	CTYRAG	2 cut(s)	754, 1449
Sse9I	AATT	9 cut(s)	600, 738, 960, 1077, 1085, 1101, 1368, 1596, 1932
SseBI	AGGCCT	1 cut(s)	73
SspMI	CTAG	4 cut(s)	12, 1746, 1782, 1793
SstI	GAGCTC	1 cut(s)	1966
StuI	AGGCCT	1 cut(s)	73
TaaI	ACNGT	8 cut(s)	24, 58, 193, 209, 485, 816, 1657, 1708
TaiI	ACGT	4 cut(s)	206, 306, 1475, 1732
TaqI	TCGA	9 cut(s)	272, 557, 677, 1024, 1450, 1510, 1580, 1841, 1930
TasI	AATT	9 cut(s)	600, 738, 960, 1077, 1085, 1101, 1368, 1596, 1932
TauI	GCSGC	8 cut(s)	144, 169, 361, 364, 510, 513, 516, 547
TfiI	GAWTC	4 cut(s)	316, 433, 529, 1067
Tru1I	TTAA	2 cut(s)	267, 1991
Tru9I	TTAA	2 cut(s)	267, 1991
TscAI	CASTG	2 cut(s)	1561, 1888
TseFI	GTSAC	5 cut(s)	218, 1468, 1768, 1777, 1885
TseI	GCWGC	3 cut(s)	280, 997, 1445
Tsp45I	GTSAC	5 cut(s)	218, 1468, 1768, 1777, 1885
TspDTI	ATGAA	9 cut(s)	48, 242, 324, 735, 866, 936, 1113, 1161, 1646
TspGWI	ACGGA	3 cut(s)	65, 880, 1498
TspRI	CASTG	2 cut(s)	1561, 1888
Van91I	CCANNNNNTGG	1 cut(s)	1547
VpaK11BI	GGWCC	5 cut(s)	178, 367, 421, 896, 1178
XagI	CCTNNNNNAGG	1 cut(s)	1895
XapI	RAATTY	2 cut(s)	1368, 1932
XbaI	TCTAGA	1 cut(s)	11
XceI	RCATGY	2 cut(s)	569, 1398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1888
XhoI	CTCGAG	1 cut(s)	1449
XmiI	GTMKAC	1 cut(s)	1789
XmnI	GAANNNNTTC	4 cut(s)	315, 802, 1089, 1740
XspI	CTAG	4 cut(s)	12, 1746, 1782, 1793
ZraI	GACGTC	1 cut(s)	1473
Zsp2I	ATGCAT	2 cut(s)	567, 804

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.