Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009148

Physical Location & Seq

Genomic Coordinates Reverse (-)

35498371 .. 35503078

Gene Structure 4708 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1293700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2007 bp

ATGGAGAGGTCTAGATCTAAACGGTACTACTATGAACAGGACTACGATACGGAGACTGTGGGTAGGACCAGGCCTCGGTACAATCACCACTACGCGAACAACAACAACCACCGCCACCGCGGAGGTGGAAGCGGCGGCGGCGGTGGTGGTGGAAGACCATCTAAGCCTCCTCAGCAGGATCCGTCGGTTACGGTAACTACGACATACCGTATTCTCTGTCACGATATGAGGGCCGGAGGGGTGATTGGGAAGTCCGGTAGCATCATTAAGTCGATTCGGCAGCACACCGGCGCATGGATCAACGTACATGAACTGATTCCGGGAGATGAGGAGCGGATTATTGAGATTACCGACACGCGGCGGCGGGACCCGGAGGGGCGAATGCCGTCCTTCTCGCCGGCTCAGGAGGCTCTTTTTTTGATCCATGAGAGGATTCTGGAGAGTGATGTTGCTGGGTTTGGCGGTGAGGAGGAGGATGAGTATGGTGGTGGGGTTAGAGGCGGCGGCGGTGGCGGTGGGAATCGAGTAGCGACGCGGTTGGTTGTGTCGAGAATGCATGTGGGATGTTTGCTGGGGAAGGGAGGGAAGATAATTGAGCAGATGAGGATGGAGACTAAGACCCAGATTAGGGTGTTGCCTAGAGATCATAATCTGCCTCGATGCGTTTCGATGTCGGAGGAGATTGTTCAGGTAGTAGGCGATCCGAATAATGTAAAGAGTGCTGTGGCAATTATTTCATCACGCTTGAGGGAGAGTCAGCATCGGGATCGCAGTCATTTCCAGGGGCGAATGCATTCACCTGAACGGTTCTTTCCTCCTGATGATGATTATGTTCCTCATATGAACAATGCAGGACGGAAGACACCCATGGATGGGGCTCCCTTTGGACCACGATTATCTAACCCCAGTATCAGAAACAACAACTATGCCCCTCGTTCATCGGGTTATCCAATTGAACATGGGTCTGCTCCCATGGCTGATAATACACAGCCCTTTTGTGAAGACCTTGTGTTTCGAATACTTTGTCCAGTTGACAAAGTTTATCTTATAGTTGGAGATTCAACCGGAATTATAGAACTGCTTCAAAATGAAATTGGTGTGGATGTCAAGGCTACTAACCCTGTGGTTGGTGAAGATGAACAGATAATCATAATCTCTTCTGAGGAGGGTCCTGATGACGAGCTGTTTCCAGCTCAGGAAGCTTTGTTGCATATTCAAACTCGCATTGTCGATCTTAGTCCAGACAAAGAGAACACTATAACTACTAGGCTACTTGTCCCAACAAGTGATATTGGATGGTTGGAGGGAAGAGAAGGGTTGTTAATGGATATGAGGCGACTTACCGGTGCAGATATACAAATTCTTCCCATAGATGATCTTCCCACGTGTGTATCAGGGGACGATGTACTTGTACAGATTGTAGGGGAAATAAAAGCAGCTCGACATGCTCTTGTTGAGATGACATCAAGAATACGGAGTTACTTATATAGAGAGTTGTTTCGAAAGGATACACCACCACCTGCTTTTGCACCAGGCCCTGCAGGTAGTGCTTTCACAATAGACCCATCTCCGAATAACACATTTCCAGTTCGTGAAGCTCATACTGGAAATGATCCTCCCATGAAAATGTATCAGAATGTGCAAACTGGGGGAATTGCACAGCCATCAAAGGATGCTGGAGGGATTGCCAATGAAACAGTAAAGCCATACGAAAGTGAACGGCGTGAAGATGCTCCGAGCTCTTTGAATAGAATGCCTGTGACGCTTGTCACTAGGAGTATACTTGAAGTTGTAATACCAGAGCAGGCAGTTTCAAAGCTCATAACGAAATCAAGAAACAAGCTTGCTCAGATCAGTGAGTTATCAGGAGCAAATGTGACCCTGGTAGAGGATAGACCGGAGGAAACACAGAAGATCATTCGAATTTCAGGTACTCCAGAGCAGGCCGAGAGAGCTCAGAGCTTGCTTCAGGGCTTTATTTTAAGCACACAAGAAGACGGCCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002831 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010286 GO:0010445 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0019222 GO:0023052 GO:0031047 GO:0031050 GO:0031053 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032870 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043900 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070878 GO:0070887 GO:0070918 GO:0071229 GO:0071310 GO:0071359 GO:0071395 GO:0071407 GO:0071495 GO:0071704 GO:0080134 GO:0090304 GO:0097159 GO:1900150 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

669

Amino Acids

73.62

Weight (kDa)

5.78

Isoelectric Point (pI)

56.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	68 - 117	5e-10	KH domain
KH_1	PF00013	179 - 246	3.1e-12	KH domain
KH_1	PF00013	595 - 657	1.6e-09	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53060 AT5G53060 AT5G53060
fragaria_vesca	FvH4_7g32010 FvH4_7g32010
malus_domestica	MD01G1222900.v1.1 MD07G1293700.v1.1
prunus_persica	Prupe.2G313500_v2.0.a1
pyrus_communis	pycom01g23230 pycom07g26650
rosa_chinensis	RchiOBHm_Chr1g0381621
rosa_laevigata	RLG00000026220
rosa_multiflora	Rmu_sc0003688.1_g000012 Rmu_sc0005891.1_g000007
rosa_roxburghii	Rroxscaffold_4G00278580
rosa_rugosa	Rorug01G0429700
rosa_samantha	Rh1AG453000 Rh1BG410000 Rh1CG424600 Rh1DG441800 Rh2CG399100
rosa_wichuraiana	Rw1G039520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1528
Acc36I	ACCTGC	2 cut(s)	1528, 1532
AccBSI	CCGCTC	1 cut(s)	334
AccI	GTMKAC	1 cut(s)	1780
AccII	CGCG	4 cut(s)	95, 120, 358, 535
AclWI	GGATC	7 cut(s)	173, 186, 305, 415, 695, 774, 1607
AcsI	RAATTY	2 cut(s)	1359, 1923
AcuI	CTGAAG	1 cut(s)	1952
AcvI	CACGTG	1 cut(s)	1386
AfaI	GTAC	6 cut(s)	26, 80, 306, 1407, 1413, 1933
AfiI	CCNNNNNNNGG	9 cut(s)	75, 294, 357, 627, 628, 872, 873, 1127, 1888
AflIII	ACRYGT	1 cut(s)	1385
AgeI	ACCGGT	1 cut(s)	1343
AgsI	TTSAA	7 cut(s)	956, 1062, 1085, 1217, 1747, 1787, 1815
AjnI	CCWGG	4 cut(s)	68, 780, 1531, 1881
Alw21I	GWGCWC	2 cut(s)	1742, 1957
Alw26I	GTCTC	2 cut(s)	47, 605
AlwI	GGATC	7 cut(s)	173, 186, 305, 415, 695, 774, 1607
AlwNI	CAGNNNCTG	1 cut(s)	1538
AoxI	GGCC	5 cut(s)	71, 231, 1534, 1944, 1999
ApeKI	GCWGC	2 cut(s)	280, 1436
ApoI	RAATTY	2 cut(s)	1359, 1923
ArsI	GACNNNNNNTTYG	2 cut(s)	1009, 1041
AsiGI	ACCGGT	1 cut(s)	1343
Asp700I	GAANNNNTTC	3 cut(s)	315, 793, 1080
AspLEI	GCGC	1 cut(s)	293
AspS9I	GGNCC	7 cut(s)	66, 231, 367, 887, 1169, 1535, 2000
AsuC2I	CCSGG	2 cut(s)	321, 371
AsuHPI	GGTGA	5 cut(s)	77, 253, 476, 789, 1142
AsuII	TTCGAA	3 cut(s)	1015, 1501, 1921
AvaII	GGWCC	4 cut(s)	66, 367, 887, 1169
BaeI	ACNNNNGTAYC	4 cut(s)	892, 925, 1374, 1407
BamHI	GGATCC	1 cut(s)	178
BanII	GRGCYC	3 cut(s)	880, 1742, 1957
BarI	GAAGNNNNNNTAC	2 cut(s)	1779, 1811
BbrPI	CACGTG	1 cut(s)	1386
BbsI	GAAGAC	4 cut(s)	160, 866, 1008, 2001
Bbv12I	GWGCWC	2 cut(s)	1742, 1957
BbvCI	CCTCAGC	1 cut(s)	171
BbvI	GCAGC	2 cut(s)	292, 1448
BccI	CCATC	6 cut(s)	166, 601, 866, 1290, 1573, 1672
BceAI	ACGGC	2 cut(s)	370, 1736
BcgI	CGANNNNNNTGC	2 cut(s)	749, 783
BciT130I	CCWGG	4 cut(s)	70, 782, 1533, 1883
BciVI	GTATCC	1 cut(s)	1501
BcnI	CCSGG	2 cut(s)	321, 371
BcoDI	GTCTC	2 cut(s)	47, 605
BfaI	CTAG	4 cut(s)	12, 639, 1266, 1773
BfmI	CTRYAG	1 cut(s)	1539
BfuAI	ACCTGC	2 cut(s)	1528, 1532
BfuI	GTATCC	1 cut(s)	1501
BglII	AGATCT	1 cut(s)	14
BisI	GCNGC	9 cut(s)	133, 136, 139, 281, 359, 362, 502, 505, 1437
BlsI	GCNGC	9 cut(s)	134, 137, 140, 282, 360, 363, 503, 506, 1438
Bme1390I	CCNGG	6 cut(s)	70, 321, 371, 782, 1533, 1883
Bme18I	GGWCC	4 cut(s)	66, 367, 887, 1169
BmgT120I	GGNCC	7 cut(s)	66, 231, 367, 887, 1169, 1535, 2000
BmiI	GGNNCC	5 cut(s)	180, 368, 369, 879, 1170
BmrFI	CCNGG	6 cut(s)	70, 321, 371, 782, 1533, 1883
BmrI	ACTGGG	2 cut(s)	900, 1656
BmsI	GCATC	5 cut(s)	270, 650, 769, 1663, 1720
BmuI	ACTGGG	2 cut(s)	900, 1656
BoxI	GACNNNNGTC	1 cut(s)	1766
BpiI	GAAGAC	4 cut(s)	160, 866, 1008, 2001
BpmI	CTGGAG	3 cut(s)	458, 1698, 1920
Bpu10I	CCTNAGC	3 cut(s)	171, 402, 1194
Bpu14I	TTCGAA	3 cut(s)	1015, 1501, 1921
BpuEI	CTTGAG	1 cut(s)	766
BpuMI	CCSGG	2 cut(s)	321, 371
BsaAI	YACGTR	1 cut(s)	1386
BsaBI	GATNNNNATC	1 cut(s)	648
BsaJI	CCNNGG	6 cut(s)	74, 118, 781, 867, 972, 1881
BsaWI	WCCGGW	4 cut(s)	254, 1064, 1343, 1897
BsaXI	ACNNNNNCTCC	4 cut(s)	464, 494, 1860, 1890
Bsc4I	CCNNNNNNNGG	9 cut(s)	75, 294, 357, 627, 628, 872, 873, 1127, 1888
Bse118I	RCCGGY	3 cut(s)	287, 397, 1343
Bse1I	ACTGG	5 cut(s)	906, 1028, 1586, 1609, 1651
Bse8I	GATNNNNATC	1 cut(s)	648
BseBI	CCWGG	4 cut(s)	70, 782, 1533, 1883
BseDI	CCNNGG	6 cut(s)	74, 118, 781, 867, 972, 1881
BseGI	GGATG	7 cut(s)	481, 569, 612, 877, 1108, 1301, 1678
BseJI	GATNNNNATC	1 cut(s)	648
BseLI	CCNNNNNNNGG	9 cut(s)	75, 294, 357, 627, 628, 872, 873, 1127, 1888
BseMII	CTCAG	6 cut(s)	185, 416, 1152, 1208, 1862, 1970
BseNI	ACTGG	5 cut(s)	906, 1028, 1586, 1609, 1651
BseRI	GAGGAG	6 cut(s)	159, 344, 482, 485, 692, 1178
BseXI	GCAGC	2 cut(s)	292, 1448
BseYI	CCCAGC	2 cut(s)	452, 571
BsgI	GTGCAG	1 cut(s)	1368
Bsh1236I	CGCG	4 cut(s)	95, 120, 358, 535
BshFI	GGCC	5 cut(s)	73, 233, 1536, 1946, 2001
BshTI	ACCGGT	1 cut(s)	1343
BsiHKAI	GWGCWC	2 cut(s)	1742, 1957
BsiSI	CCGG	9 cut(s)	234, 255, 288, 320, 371, 398, 1065, 1344, 1898
BslFI	GGGAC	3 cut(s)	380, 1262, 1412
BslI	CCNNNNNNNGG	9 cut(s)	75, 294, 357, 627, 628, 872, 873, 1127, 1888
BsmAI	GTCTC	2 cut(s)	47, 605
BsmFI	GGGAC	3 cut(s)	380, 1262, 1412
BsmI	GAATGC	5 cut(s)	387, 558, 793, 795, 1758
BsnI	GGCC	5 cut(s)	73, 233, 1536, 1946, 2001
Bsp119I	TTCGAA	3 cut(s)	1015, 1501, 1921
Bsp1286I	GDGCHC	3 cut(s)	880, 1742, 1957
Bsp1407I	TGTACA	1 cut(s)	1411
Bsp19I	CCATGG	2 cut(s)	867, 972
BspANI	GGCC	5 cut(s)	73, 233, 1536, 1946, 2001
BspCNI	CTCAG	6 cut(s)	184, 415, 1153, 1207, 1861, 1969
BspFNI	CGCG	4 cut(s)	95, 120, 358, 535
BspLI	GGNNCC	5 cut(s)	180, 368, 369, 879, 1170
BspMAI	CTGCAG	1 cut(s)	1543
BspMI	ACCTGC	2 cut(s)	1528, 1532
BspPI	GGATC	7 cut(s)	173, 186, 305, 415, 695, 774, 1607
BspT104I	TTCGAA	3 cut(s)	1015, 1501, 1921
BsrBI	CCGCTC	1 cut(s)	334
BsrFI	RCCGGY	3 cut(s)	287, 397, 1343
BsrGI	TGTACA	1 cut(s)	1411
BsrI	ACTGG	5 cut(s)	906, 1028, 1586, 1609, 1651
BssAI	RCCGGY	3 cut(s)	287, 397, 1343
BssECI	CCNNGG	6 cut(s)	74, 118, 781, 867, 972, 1881
BssNAI	GTATAC	1 cut(s)	1781
BssT1I	CCWWGG	2 cut(s)	867, 972
Bst1107I	GTATAC	1 cut(s)	1781
Bst2UI	CCWGG	4 cut(s)	70, 782, 1533, 1883
Bst4CI	ACNGT	6 cut(s)	24, 58, 193, 209, 807, 1699
Bst6I	CTCTTC	2 cut(s)	1162, 1303
BstAPI	GCANNNNNTGC	1 cut(s)	1547
BstAUI	TGTACA	1 cut(s)	1411
BstBAI	YACGTR	1 cut(s)	1386
BstBI	TTCGAA	3 cut(s)	1015, 1501, 1921
BstC8I	GCNNGC	5 cut(s)	399, 1806, 1845, 1944, 1964
BstDSI	CCRYGG	3 cut(s)	118, 867, 972
BstENI	CCTNNNNNAGG	1 cut(s)	1886
BstF5I	GGATG	7 cut(s)	481, 569, 612, 877, 1108, 1301, 1678
BstFNI	CGCG	4 cut(s)	95, 120, 358, 535
BstHHI	GCGC	1 cut(s)	293
BstMAI	GTCTC	2 cut(s)	47, 605
BstNI	CCWGG	4 cut(s)	70, 782, 1533, 1883
BstNSI	RCATGY	2 cut(s)	560, 1448
BstPAI	GACNNNNGTC	1 cut(s)	1766
BstSCI	CCNGG	6 cut(s)	68, 319, 369, 780, 1531, 1881
BstSFI	CTRYAG	1 cut(s)	1539
BstUI	CGCG	4 cut(s)	95, 120, 358, 535
BstV1I	GCAGC	2 cut(s)	292, 1448
BstV2I	GAAGAC	4 cut(s)	160, 866, 1008, 2001
BstX2I	RGATCY	2 cut(s)	14, 178
BstYI	RGATCY	2 cut(s)	14, 178
BstZ17I	GTATAC	1 cut(s)	1781
BsuI	GTATCC	1 cut(s)	1501
BsuRI	GGCC	5 cut(s)	73, 233, 1536, 1946, 2001
BtgI	CCRYGG	3 cut(s)	118, 867, 972
BtsCI	GGATG	7 cut(s)	481, 569, 612, 877, 1108, 1301, 1678
BtsIMutI	CAGTG	1 cut(s)	1861
BveI	ACCTGC	2 cut(s)	1528, 1532
Cac8I	GCNNGC	5 cut(s)	399, 1806, 1845, 1944, 1964
CaiI	CAGNNNCTG	1 cut(s)	1538
CfoI	GCGC	1 cut(s)	293
Cfr10I	RCCGGY	3 cut(s)	287, 397, 1343
Cfr13I	GGNCC	7 cut(s)	66, 231, 367, 887, 1169, 1535, 2000
Cfr42I	CCGCGG	1 cut(s)	121
CseI	GACGC	2 cut(s)	541, 1771
Csp6I	GTAC	6 cut(s)	25, 79, 305, 1406, 1412, 1932
CspAI	ACCGGT	1 cut(s)	1343
CviAII	CATG	9 cut(s)	294, 308, 425, 557, 868, 959, 973, 1445, 1621
CviQI	GTAC	6 cut(s)	25, 79, 305, 1406, 1412, 1932
Eam1104I	CTCTTC	2 cut(s)	1162, 1303
EarI	CTCTTC	2 cut(s)	1162, 1303
Ecl136II	GAGCTC	2 cut(s)	1740, 1955
Eco130I	CCWWGG	2 cut(s)	867, 972
Eco147I	AGGCCT	1 cut(s)	73
Eco24I	GRGCYC	3 cut(s)	880, 1742, 1957
Eco47I	GGWCC	4 cut(s)	66, 367, 887, 1169
Eco53kI	GAGCTC	2 cut(s)	1740, 1955
Eco57I	CTGAAG	1 cut(s)	1952
Eco72I	CACGTG	1 cut(s)	1386
EcoICRI	GAGCTC	2 cut(s)	1740, 1955
EcoNI	CCTNNNNNAGG	1 cut(s)	1886
EcoO109I	RGGNCCY	3 cut(s)	367, 1169, 1535
EcoRII	CCWGG	4 cut(s)	68, 780, 1531, 1881
EcoT14I	CCWWGG	2 cut(s)	867, 972
EcoT22I	ATGCAT	2 cut(s)	558, 795
EcoT38I	GRGCYC	3 cut(s)	880, 1742, 1957
ErhI	CCWWGG	2 cut(s)	867, 972
FaeI	CATG	9 cut(s)	297, 311, 428, 560, 871, 962, 976, 1448, 1624
FalI	AAGNNNNNCTT	2 cut(s)	1029, 1061
FaqI	GGGAC	3 cut(s)	380, 1262, 1412
FatI	CATG	9 cut(s)	293, 307, 424, 556, 867, 958, 972, 1444, 1620
FauI	CCCGC	1 cut(s)	357
FauNDI	CATATG	1 cut(s)	840
FblI	GTMKAC	1 cut(s)	1780
Fnu4HI	GCNGC	9 cut(s)	133, 136, 139, 281, 359, 362, 502, 505, 1437
FokI	GGATG	7 cut(s)	488, 576, 619, 884, 1115, 1308, 1685
FriOI	GRGCYC	3 cut(s)	880, 1742, 1957
Fsp4HI	GCNGC	9 cut(s)	133, 136, 139, 281, 359, 362, 502, 505, 1437
FspBI	CTAG	4 cut(s)	12, 639, 1266, 1773
GlaI	GCGC	1 cut(s)	292
GluI	GCNGC	9 cut(s)	133, 136, 139, 281, 359, 362, 502, 505, 1437
GsaI	CCCAGC	2 cut(s)	456, 575
GsuI	CTGGAG	3 cut(s)	458, 1698, 1920
HaeIII	GGCC	5 cut(s)	73, 233, 1536, 1946, 2001
HapII	CCGG	9 cut(s)	234, 255, 288, 320, 371, 398, 1065, 1344, 1898
HgaI	GACGC	2 cut(s)	541, 1771
HhaI	GCGC	1 cut(s)	293
Hin1II	CATG	9 cut(s)	297, 311, 428, 560, 871, 962, 976, 1448, 1624
Hin6I	GCGC	1 cut(s)	291
HinP1I	GCGC	1 cut(s)	291
HincII	GTYRAC	1 cut(s)	1033
HindII	GTYRAC	1 cut(s)	1033
HindIII	AAGCTT	2 cut(s)	1200, 1841
HinfI	GANTC	6 cut(s)	274, 316, 433, 520, 754, 1058
HpaII	CCGG	9 cut(s)	234, 255, 288, 320, 371, 398, 1065, 1344, 1898
HphI	GGTGA	5 cut(s)	77, 253, 476, 789, 1142
Hpy166II	GTNNAC	3 cut(s)	1033, 1718, 1781
Hpy188I	TCNGA	9 cut(s)	676, 705, 914, 1162, 1572, 1635, 1737, 1851, 1959
Hpy8I	GTNNAC	3 cut(s)	1033, 1718, 1781
Hpy99I	CGWCG	2 cut(s)	187, 535
HpyAV	CCTTC	3 cut(s)	400, 571, 1307
HpyCH4III	ACNGT	6 cut(s)	24, 58, 193, 209, 807, 1699
HpyCH4IV	ACGT	2 cut(s)	303, 1385
HpyCH4V	TGCA	9 cut(s)	556, 793, 851, 1210, 1349, 1529, 1541, 1642, 1658
HpySE526I	ACGT	2 cut(s)	303, 1385
Hsp92II	CATG	9 cut(s)	297, 311, 428, 560, 871, 962, 976, 1448, 1624
HspAI	GCGC	1 cut(s)	291
KflI	GGGWCCC	1 cut(s)	367
KroI	GCCGGC	1 cut(s)	397
KroNI	GCCGGC	1 cut(s)	399
KspI	CCGCGG	1 cut(s)	121
LmnI	GCTCC	5 cut(s)	331, 883, 973, 1738, 1868
Lsp1109I	GCAGC	2 cut(s)	292, 1448
LweI	GCATC	5 cut(s)	270, 650, 769, 1663, 1720
MaeI	CTAG	4 cut(s)	12, 639, 1266, 1773
MaeII	ACGT	2 cut(s)	303, 1385
MaeIII	GTNAC	7 cut(s)	187, 193, 218, 1478, 1759, 1768, 1876
MbiI	CCGCTC	1 cut(s)	334
MfeI	CAATTG	1 cut(s)	951
MflI	RGATCY	2 cut(s)	14, 178
MhlI	GDGCHC	3 cut(s)	880, 1742, 1957
MluCI	AATT	8 cut(s)	591, 729, 951, 1068, 1092, 1359, 1653, 1923
MlyI	GAGTC	1 cut(s)	763
MmeI	TCCRAC	3 cut(s)	654, 1033, 1281
Mph1103I	ATGCAT	2 cut(s)	558, 795
MroNI	GCCGGC	1 cut(s)	397
MroXI	GAANNNNTTC	3 cut(s)	315, 793, 1080
MseI	TTAA	3 cut(s)	267, 1322, 1982
MslI	CAYNNNNRTG	1 cut(s)	1625
MspA1I	CMGCKG	1 cut(s)	120
MspI	CCGG	9 cut(s)	234, 255, 288, 320, 371, 398, 1065, 1344, 1898
MspR9I	CCNGG	6 cut(s)	70, 321, 371, 782, 1533, 1883
MunI	CAATTG	1 cut(s)	951
Mva1269I	GAATGC	5 cut(s)	387, 558, 793, 795, 1758
MvaI	CCWGG	4 cut(s)	70, 782, 1533, 1883
MvnI	CGCG	4 cut(s)	95, 120, 358, 535
NaeI	GCCGGC	1 cut(s)	399
NciI	CCSGG	2 cut(s)	321, 371
NcoI	CCATGG	2 cut(s)	867, 972
NdeI	CATATG	1 cut(s)	840
NgoMIV	GCCGGC	1 cut(s)	397
NlaIII	CATG	9 cut(s)	297, 311, 428, 560, 871, 962, 976, 1448, 1624
NlaIV	GGNNCC	5 cut(s)	180, 368, 369, 879, 1170
NmeAIII	GCCGAG	1 cut(s)	1972
NmuCI	GTSAC	4 cut(s)	218, 1759, 1768, 1876
NsiI	ATGCAT	2 cut(s)	558, 795
NspI	RCATGY	2 cut(s)	560, 1448
NspV	TTCGAA	3 cut(s)	1015, 1501, 1921
PaqCI	CACCTGC	1 cut(s)	1528
PceI	AGGCCT	1 cut(s)	73
PcsI	WCGNNNNNNNCGW	1 cut(s)	197
PctI	GAATGC	5 cut(s)	387, 558, 793, 795, 1758
PdiI	GCCGGC	1 cut(s)	399
PdmI	GAANNNNTTC	3 cut(s)	315, 793, 1080
PfeI	GAWTC	5 cut(s)	274, 316, 433, 520, 1058
PfoI	TCCNGGA	1 cut(s)	319
PinAI	ACCGGT	1 cut(s)	1343
PkrI	GCNGC	9 cut(s)	134, 137, 140, 282, 360, 363, 503, 506, 1438
PleI	GAGTC	1 cut(s)	762
PmaCI	CACGTG	1 cut(s)	1386
PmlI	CACGTG	1 cut(s)	1386
PpsI	GAGTC	1 cut(s)	762
Ppu21I	YACGTR	1 cut(s)	1386
PpuMI	RGGWCCY	2 cut(s)	367, 1169
PshAI	GACNNNNGTC	1 cut(s)	1766
Psp124BI	GAGCTC	2 cut(s)	1742, 1957
Psp5II	RGGWCCY	2 cut(s)	367, 1169
Psp6I	CCWGG	4 cut(s)	68, 780, 1531, 1881
PspCI	CACGTG	1 cut(s)	1386
PspFI	CCCAGC	2 cut(s)	452, 571
PspGI	CCWGG	4 cut(s)	68, 780, 1531, 1881
PspN4I	GGNNCC	5 cut(s)	180, 368, 369, 879, 1170
PspPI	GGNCC	7 cut(s)	66, 231, 367, 887, 1169, 1535, 2000
PspPPI	RGGWCCY	2 cut(s)	367, 1169
PstI	CTGCAG	1 cut(s)	1543
PstNI	CAGNNNCTG	1 cut(s)	1538
PsuI	RGATCY	2 cut(s)	14, 178
RsaI	GTAC	6 cut(s)	26, 80, 306, 1407, 1413, 1933
RsaNI	GTAC	6 cut(s)	25, 79, 305, 1406, 1412, 1932
RseI	CAYNNNNRTG	1 cut(s)	1625
SacI	GAGCTC	2 cut(s)	1742, 1957
SacII	CCGCGG	1 cut(s)	121
SaqAI	TTAA	3 cut(s)	267, 1322, 1982
SatI	GCNGC	9 cut(s)	133, 136, 139, 281, 359, 362, 502, 505, 1437
Sau96I	GGNCC	7 cut(s)	66, 231, 367, 887, 1169, 1535, 2000
SbfI	CCTGCAGG	1 cut(s)	1543
SchI	GAGTC	1 cut(s)	763
ScrFI	CCNGG	6 cut(s)	70, 321, 371, 782, 1533, 1883
SdaI	CCTGCAGG	1 cut(s)	1543
SduI	GDGCHC	3 cut(s)	880, 1742, 1957
SfaNI	GCATC	5 cut(s)	270, 650, 769, 1663, 1720
SfcI	CTRYAG	1 cut(s)	1539
Sfr303I	CCGCGG	1 cut(s)	121
SfuI	TTCGAA	3 cut(s)	1015, 1501, 1921
SgrAI	CRCCGGYG	1 cut(s)	287
SgrBI	CCGCGG	1 cut(s)	121
SinI	GGWCC	4 cut(s)	66, 367, 887, 1169
SmiMI	CAYNNNNRTG	1 cut(s)	1625
SmlI	CTYRAG	1 cut(s)	745
SmoI	CTYRAG	1 cut(s)	745
Sse8387I	CCTGCAGG	1 cut(s)	1543
Sse9I	AATT	8 cut(s)	591, 729, 951, 1068, 1092, 1359, 1653, 1923
SseBI	AGGCCT	1 cut(s)	73
SspMI	CTAG	4 cut(s)	12, 639, 1266, 1773
SstI	GAGCTC	2 cut(s)	1742, 1957
StuI	AGGCCT	1 cut(s)	73
StyD4I	CCNGG	6 cut(s)	68, 319, 369, 780, 1531, 1881
StyI	CCWWGG	2 cut(s)	867, 972
TaaI	ACNGT	6 cut(s)	24, 58, 193, 209, 807, 1699
TaiI	ACGT	2 cut(s)	306, 1388
TasI	AATT	8 cut(s)	591, 729, 951, 1068, 1092, 1359, 1653, 1923
TatI	WGTACW	2 cut(s)	1405, 1411
TauI	GCSGC	7 cut(s)	135, 138, 141, 361, 364, 504, 507
TfiI	GAWTC	5 cut(s)	274, 316, 433, 520, 1058
Tru1I	TTAA	3 cut(s)	267, 1322, 1982
Tru9I	TTAA	3 cut(s)	267, 1322, 1982
TscAI	CASTG	1 cut(s)	1861
TseFI	GTSAC	4 cut(s)	218, 1759, 1768, 1876
TseI	GCWGC	2 cut(s)	280, 1436
Tsp45I	GTSAC	4 cut(s)	218, 1759, 1768, 1876
TspDTI	ATGAA	9 cut(s)	48, 324, 726, 857, 927, 1104, 1152, 1637, 1707
TspGWI	ACGGA	4 cut(s)	65, 171, 871, 1489
TspRI	CASTG	1 cut(s)	1861
VpaK11BI	GGWCC	4 cut(s)	66, 367, 887, 1169
XagI	CCTNNNNNAGG	1 cut(s)	1886
XapI	RAATTY	2 cut(s)	1359, 1923
XbaI	TCTAGA	1 cut(s)	11
XceI	RCATGY	2 cut(s)	560, 1448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	122
XmiI	GTMKAC	1 cut(s)	1780
XmnI	GAANNNNTTC	3 cut(s)	315, 793, 1080
XspI	CTAG	4 cut(s)	12, 639, 1266, 1773
Zsp2I	ATGCAT	2 cut(s)	558, 795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.