Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014029

Physical Location & Seq

Genomic Coordinates Forward (+)

31728955 .. 31730100

Gene Structure 1146 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1227600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 480 bp

ATGTGGAGAGGAGGCCAAGGAGTTCGGCTGTGGACATTTCTCCTTTTGGTAAGTCTATTGGATCCCCTCTCCCCAGTGAGAACAATGCGGCAAATGCTGGACACAGTGGACCGGCTTTTCGGAGACACGGTGGCATTCCCCGGCAGAACCAAAGCAGCAGGGGAAGTTCGTGCGCCATGGGACATCAAAGACGACGAACACGAAATCAAAATGCGCTTTGACATGCCGGGGCTCTCGAAGGAGGACGTGAAGGTGTCGGTTGAGGACGATGTGTTAGTTATAAAGGGGGAGCAAAAGAAGGAAGAAGGTGGGGACGATTCGTGGTCGAGCAGGAGCTATAGCTCCTACAATACCCGGCTTCAGCTGCCGGATAATTCAGAGAAGGACAAGATCAAGGCGGAGCTCAAGAATGGGGTTCTTTACATAGCTATTCCTAAGGCCAAAGTTGAACGCAAGGTTATTGATGTCGCAATACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006950 GO:0006979 GO:0006996 GO:0008150 GO:0009266 GO:0009295 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009657 GO:0009658 GO:0009889 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032991 GO:0042221 GO:0042493 GO:0042542 GO:0042644 GO:0042646 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043621 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0101031 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

HSP20

Protein Analysis

160

Amino Acids

18.05

Weight (kDa)

5.94

Isoelectric Point (pI)

30.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP20	PF00011	61 - 159	1.4e-30	Hsp20/alpha crystallin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0014029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27670
fragaria_vesca	FvH4_6g02120 FvH4_7g32800
malus_domestica	MD01G1227600.v1.1 MD07G1298000.v1.1
prunus_persica	Prupe.2G317700_v2.0.a1
pyrus_communis	pycom07g27040
rosa_chinensis	RchiOBHm_Chr1g0382761
rosa_laevigata	RLG00000026163
rosa_multiflora	Rmu_co8395491.1_g000001
rosa_roxburghii	Rroxscaffold_4G00277930
rosa_rugosa	Rorug01G0435600
rosa_samantha	Rh1AG460800 Rh1BG417600 Rh1CG431200 Rh1DG449800
rosa_wichuraiana	Rw1G041080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	281
AciI	CCGC	2 cut(s)	88, 398
AclWI	GGATC	2 cut(s)	56, 69
AcuI	CTGAAG	1 cut(s)	344
AgsI	TTSAA	1 cut(s)	449
AjiI	CACGTC	1 cut(s)	247
AluBI	AGCT	5 cut(s)	336, 342, 364, 403, 428
AluI	AGCT	5 cut(s)	336, 342, 364, 403, 428
Alw21I	GWGCWC	1 cut(s)	405
Alw26I	GTCTC	1 cut(s)	117
AlwI	GGATC	2 cut(s)	56, 69
AoxI	GGCC	2 cut(s)	13, 438
ApeKI	GCWGC	2 cut(s)	155, 364
ArsI	GACNNNNNNTTYG	2 cut(s)	101, 133
AspLEI	GCGC	2 cut(s)	175, 216
AspS9I	GGNCC	1 cut(s)	109
AsuC2I	CCSGG	3 cut(s)	141, 228, 355
AvaII	GGWCC	1 cut(s)	109
AxyI	CCTNAGG	1 cut(s)	435
BamHI	GGATCC	1 cut(s)	61
BanII	GRGCYC	2 cut(s)	234, 405
Bbv12I	GWGCWC	1 cut(s)	405
BbvI	GCAGC	2 cut(s)	167, 351
BcnI	CCSGG	3 cut(s)	141, 228, 355
BcoDI	GTCTC	1 cut(s)	117
BfmI	CTRYAG	1 cut(s)	337
BisI	GCNGC	3 cut(s)	89, 156, 365
BlsI	GCNGC	3 cut(s)	90, 157, 366
Bme1390I	CCNGG	3 cut(s)	141, 228, 355
Bme18I	GGWCC	1 cut(s)	109
BmgBI	CACGTC	1 cut(s)	247
BmgT120I	GGNCC	1 cut(s)	109
BmiI	GGNNCC	1 cut(s)	63
BmrFI	CCNGG	3 cut(s)	141, 228, 355
BmrI	ACTGGG	1 cut(s)	68
BmuI	ACTGGG	1 cut(s)	68
BpuEI	CTTGAG	1 cut(s)	389
BpuMI	CCSGG	3 cut(s)	141, 228, 355
BsaJI	CCNNGG	4 cut(s)	16, 139, 176, 227
BsaXI	ACNNNNNCTCC	2 cut(s)	114, 144
Bse118I	RCCGGY	1 cut(s)	111
Bse1I	ACTGG	1 cut(s)	74
Bse21I	CCTNAGG	1 cut(s)	435
BseDI	CCNNGG	4 cut(s)	16, 139, 176, 227
BseNI	ACTGG	1 cut(s)	74
BseRI	GAGGAG	1 cut(s)	24
BseXI	GCAGC	2 cut(s)	167, 351
BshFI	GGCC	2 cut(s)	15, 440
BsiHKAI	GWGCWC	1 cut(s)	405
BsiSI	CCGG	5 cut(s)	112, 141, 227, 355, 368
BslFI	GGGAC	2 cut(s)	194, 326
BsmAI	GTCTC	1 cut(s)	117
BsmFI	GGGAC	2 cut(s)	194, 326
BsmI	GAATGC	1 cut(s)	134
BsnI	GGCC	2 cut(s)	15, 440
Bsp1286I	GDGCHC	2 cut(s)	234, 405
Bsp143I	GATC	2 cut(s)	61, 390
Bsp19I	CCATGG	1 cut(s)	176
BspACI	CCGC	2 cut(s)	88, 398
BspANI	GGCC	2 cut(s)	15, 440
BspLI	GGNNCC	1 cut(s)	63
BspPI	GGATC	2 cut(s)	56, 69
BsrFI	RCCGGY	1 cut(s)	111
BsrI	ACTGG	1 cut(s)	74
BssAI	RCCGGY	1 cut(s)	111
BssECI	CCNNGG	4 cut(s)	16, 139, 176, 227
BssMI	GATC	2 cut(s)	61, 390
BssT1I	CCWWGG	2 cut(s)	16, 176
Bst4CI	ACNGT	3 cut(s)	106, 130, 477
BstDEI	CTNAG	1 cut(s)	435
BstDSI	CCRYGG	1 cut(s)	176
BstHHI	GCGC	2 cut(s)	175, 216
BstKTI	GATC	2 cut(s)	64, 393
BstMAI	GTCTC	1 cut(s)	117
BstMBI	GATC	2 cut(s)	61, 390
BstMWI	GCNNNNNNNGC	2 cut(s)	94, 364
BstNSI	RCATGY	1 cut(s)	226
BstSCI	CCNGG	3 cut(s)	139, 226, 353
BstSFI	CTRYAG	1 cut(s)	337
BstV1I	GCAGC	2 cut(s)	167, 351
BstX2I	RGATCY	1 cut(s)	61
BstYI	RGATCY	1 cut(s)	61
Bsu36I	CCTNAGG	1 cut(s)	435
BsuRI	GGCC	2 cut(s)	15, 440
BtgI	CCRYGG	1 cut(s)	176
BtrI	CACGTC	1 cut(s)	247
BtsIMutI	CAGTG	2 cut(s)	81, 111
CfoI	GCGC	2 cut(s)	175, 216
Cfr10I	RCCGGY	1 cut(s)	111
Cfr13I	GGNCC	1 cut(s)	109
CviAII	CATG	2 cut(s)	177, 223
DdeI	CTNAG	1 cut(s)	435
DpnI	GATC	2 cut(s)	63, 392
DpnII	GATC	2 cut(s)	61, 390
EciI	GGCGGA	1 cut(s)	413
Ecl136II	GAGCTC	1 cut(s)	403
Eco130I	CCWWGG	2 cut(s)	16, 176
Eco24I	GRGCYC	2 cut(s)	234, 405
Eco47I	GGWCC	1 cut(s)	109
Eco53kI	GAGCTC	1 cut(s)	403
Eco57I	CTGAAG	1 cut(s)	344
Eco81I	CCTNAGG	1 cut(s)	435
EcoICRI	GAGCTC	1 cut(s)	403
EcoT14I	CCWWGG	2 cut(s)	16, 176
EcoT38I	GRGCYC	2 cut(s)	234, 405
ErhI	CCWWGG	2 cut(s)	16, 176
FaeI	CATG	2 cut(s)	180, 226
FaiI	YATR	5 cut(s)	178, 224, 281, 339, 425
FaqI	GGGAC	2 cut(s)	194, 326
FatI	CATG	2 cut(s)	176, 222
Fnu4HI	GCNGC	3 cut(s)	89, 156, 365
FriOI	GRGCYC	2 cut(s)	234, 405
Fsp4HI	GCNGC	3 cut(s)	89, 156, 365
GlaI	GCGC	2 cut(s)	174, 215
GluI	GCNGC	3 cut(s)	89, 156, 365
HaeIII	GGCC	2 cut(s)	15, 440
HapII	CCGG	5 cut(s)	112, 141, 227, 355, 368
HhaI	GCGC	2 cut(s)	175, 216
Hin1II	CATG	2 cut(s)	180, 226
Hin6I	GCGC	2 cut(s)	173, 214
HinP1I	GCGC	2 cut(s)	173, 214
HinfI	GANTC	1 cut(s)	317
HpaII	CCGG	5 cut(s)	112, 141, 227, 355, 368
Hpy166II	GTNNAC	2 cut(s)	33, 109
Hpy188I	TCNGA	2 cut(s)	122, 379
Hpy188III	TCNNGA	2 cut(s)	235, 406
Hpy8I	GTNNAC	2 cut(s)	33, 109
Hpy99I	CGWCG	1 cut(s)	197
HpyAV	CCTTC	5 cut(s)	232, 244, 292, 299, 376
HpyCH4III	ACNGT	3 cut(s)	106, 130, 477
HpyCH4IV	ACGT	1 cut(s)	246
HpyF10VI	GCNNNNNNNGC	2 cut(s)	94, 364
HpyF3I	CTNAG	1 cut(s)	435
HpySE526I	ACGT	1 cut(s)	246
Hsp92II	CATG	2 cut(s)	180, 226
HspAI	GCGC	2 cut(s)	173, 214
Kzo9I	GATC	2 cut(s)	61, 390
LmnI	GCTCC	4 cut(s)	289, 333, 347, 400
LpnPI	CCDG	9 cut(s)	83, 87, 125, 144, 154, 240, 316, 368, 381
Lsp1109I	GCAGC	2 cut(s)	167, 351
MaeII	ACGT	1 cut(s)	246
MalI	GATC	2 cut(s)	63, 392
MboI	GATC	2 cut(s)	61, 390
MboII	GAAGA	1 cut(s)	314
MflI	RGATCY	1 cut(s)	61
MhlI	GDGCHC	2 cut(s)	234, 405
MluCI	AATT	1 cut(s)	373
MnlI	CCTC	4 cut(s)	5, 77, 235, 256
MspA1I	CMGCKG	1 cut(s)	364
MspI	CCGG	5 cut(s)	112, 141, 227, 355, 368
MspR9I	CCNGG	3 cut(s)	141, 228, 355
Mva1269I	GAATGC	1 cut(s)	134
MwoI	GCNNNNNNNGC	2 cut(s)	94, 364
NciI	CCSGG	3 cut(s)	141, 228, 355
NcoI	CCATGG	1 cut(s)	176
NdeII	GATC	2 cut(s)	61, 390
NlaIII	CATG	2 cut(s)	180, 226
NlaIV	GGNNCC	1 cut(s)	63
NspI	RCATGY	1 cut(s)	226
PcsI	WCGNNNNNNNCGW	1 cut(s)	198
PctI	GAATGC	1 cut(s)	134
PfeI	GAWTC	1 cut(s)	317
PkrI	GCNGC	3 cut(s)	90, 157, 366
PsiI	TTATAA	1 cut(s)	281
Psp124BI	GAGCTC	1 cut(s)	405
PspN4I	GGNNCC	1 cut(s)	63
PspPI	GGNCC	1 cut(s)	109
PsuI	RGATCY	1 cut(s)	61
PvuII	CAGCTG	1 cut(s)	364
SacI	GAGCTC	1 cut(s)	405
SatI	GCNGC	3 cut(s)	89, 156, 365
Sau3AI	GATC	2 cut(s)	61, 390
Sau96I	GGNCC	1 cut(s)	109
ScrFI	CCNGG	3 cut(s)	141, 228, 355
SduI	GDGCHC	2 cut(s)	234, 405
SetI	ASST	9 cut(s)	249, 255, 310, 338, 344, 366, 405, 430, 459
SfcI	CTRYAG	1 cut(s)	337
SinI	GGWCC	1 cut(s)	109
SmlI	CTYRAG	1 cut(s)	404
SmoI	CTYRAG	1 cut(s)	404
Sse9I	AATT	1 cut(s)	373
SsiI	CCGC	2 cut(s)	88, 398
SstI	GAGCTC	1 cut(s)	405
StyD4I	CCNGG	3 cut(s)	139, 226, 353
StyI	CCWWGG	2 cut(s)	16, 176
TaaI	ACNGT	3 cut(s)	106, 130, 477
TaiI	ACGT	1 cut(s)	249
TaqI	TCGA	2 cut(s)	236, 326
TasI	AATT	1 cut(s)	373
TauI	GCSGC	1 cut(s)	91
TfiI	GAWTC	1 cut(s)	317
TscAI	CASTG	2 cut(s)	81, 111
TseI	GCWGC	2 cut(s)	155, 364
TspRI	CASTG	2 cut(s)	81, 111
VpaK11BI	GGWCC	1 cut(s)	109
XceI	RCATGY	1 cut(s)	226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.