Rh1CG431200

small heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0014029
Physical Location & Seq
Forward (+)
68829555 .. 68830336
782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG431200.1

Sequence Viewer

Length: 678 bp
ATGGCTTCAACATTGGCCTCGTCATCACCATCACCTTTTGTAGCAAGCAAAGCATTGGCTTCAGCTAAAGCAAATGTTATTTCGCGCTCAGCCAGCTTTCCTTCACCACCACGTAGACTGCCGGCGCTGAGAGCTCAGGCCGGTGGAGATGGCAATGTTGACGTGGCTGTTAATCAGGGTAGTAACCAAGGGACTCAAATGGAGAAGAGGCCTAGCTCTAGGTTGGCTGTGGATGTTTCACCTTTTGGTTTATTAGACCCCATGTCACCAATGAGGACTATGCGACAGATGCTGGACACAATGGACCGTATTTTCGAAGACACTATGATACTCCCCGGCAGCAGAAGCCGATCAGGAGGCGAGGTTCGTGCTCCTTGGGACATCAGAGACGACGAGAATGAGATCAAAATGCGGTTTGACATGCCAGGACTCTCTAAAGAGGATGTCAAGGTGTCCATTGAGGATGATGTGCTTGTTATAAAGGGTGAGCGAAACAAGGAAGAGAACAAGGACGATGCGTGGTTTAGTAGGAGCTTCAGTTCCTACAACACACGCCTTCAGCTTCCTGATAACTGTGACAAGAACAAAGTGAACGCAGAGCTCAAGAATGGTGTTCTCTACATTGCCATTCCTAAGACCAAAGTGGAGCGCAATGTCGTTGATGTAGAAATCAAGTGA

Protein Analysis

225

Amino Acids

24.86

Weight (kDa)

7.77

Isoelectric Point (pI)

61.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 127 - 224 1.4e-28 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014029)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27670
fragaria_vesca FvH4_6g02120 FvH4_7g32800
malus_domestica MD01G1227600.v1.1 MD07G1298000.v1.1
prunus_persica Prupe.2G317700_v2.0.a1
pyrus_communis pycom07g27040
rosa_chinensis RchiOBHm_Chr1g0382761
rosa_laevigata RLG00000026163
rosa_multiflora Rmu_co8395491.1_g000001
rosa_roxburghii Rroxscaffold_4G00277930
rosa_rugosa Rorug01G0435600
rosa_samantha Rh1AG460800 Rh1BG417600 Rh1CG431200 Rh1DG449800
rosa_wichuraiana Rw1G041080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AccI GTMKAC 1 cut(s) 115
AccII CGCG 1 cut(s) 85
AciI CCGC 1 cut(s) 412
AcuI CTGAAG 3 cut(s) 45, 520, 542
AgsI TTSAA 1 cut(s) 9
AhdI GACNNNNNGTC 1 cut(s) 262
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 1 cut(s) 424
AloI GAACNNNNNNTCC 4 cut(s) 348, 380, 523, 555
AluBI AGCT 7 cut(s) 65, 96, 134, 216, 534, 562, 601
AluI AGCT 7 cut(s) 65, 96, 134, 216, 534, 562, 601
Alw21I GWGCWC 3 cut(s) 136, 373, 603
Alw26I GTCTC 1 cut(s) 381
AlwNI CAGNNNCTG 1 cut(s) 292
AoxI GGCC 3 cut(s) 15, 138, 209
ApeKI GCWGC 1 cut(s) 339
ArsI GACNNNNNNTTYG 2 cut(s) 296, 328
AspLEI GCGC 3 cut(s) 87, 127, 651
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 6 cut(s) 18, 24, 96, 231, 258, 497
AsuII TTCGAA 1 cut(s) 315
AvaII GGWCC 1 cut(s) 304
BanII GRGCYC 2 cut(s) 136, 603
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 3 cut(s) 136, 373, 603
BbvI GCAGC 1 cut(s) 351
BccI CCATC 2 cut(s) 37, 143
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 426
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 2 cut(s) 213, 219
BfoI RGCGCY 1 cut(s) 128
BisI GCNGC 1 cut(s) 340
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 336, 426
Bme18I GGWCC 1 cut(s) 304
BmeRI GACNNNNNGTC 1 cut(s) 262
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 2 cut(s) 336, 426
BmsI GCATC 2 cut(s) 279, 505
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 135
Bpu1102I GCTNAGC 1 cut(s) 88
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 587
BpuMI CCSGG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 187, 334, 374
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 523, 553
Bse118I RCCGGY 2 cut(s) 121, 140
Bse3DI GCAATG 3 cut(s) 160, 621, 658
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 187, 334, 374
BseGI GGATG 3 cut(s) 238, 448, 469
BseMI GCAATG 3 cut(s) 160, 621, 658
BseMII CTCAG 3 cut(s) 102, 119, 149
BseXI GCAGC 1 cut(s) 351
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 3 cut(s) 17, 140, 211
BsiHKAI GWGCWC 3 cut(s) 136, 373, 603
BsiSI CCGG 3 cut(s) 122, 141, 336
BslFI GGGAC 2 cut(s) 205, 392
BsmAI GTCTC 1 cut(s) 381
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 205, 392
BsnI GGCC 3 cut(s) 17, 140, 211
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 3 cut(s) 136, 373, 603
Bsp143I GATC 2 cut(s) 350, 402
Bsp1720I GCTNAGC 1 cut(s) 88
BspACI CCGC 1 cut(s) 412
BspANI GGCC 3 cut(s) 17, 140, 211
BspCNI CTCAG 3 cut(s) 101, 120, 148
BspFNI CGCG 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 315
BsrDI GCAATG 3 cut(s) 160, 621, 658
BsrFI RCCGGY 2 cut(s) 121, 140
BssAI RCCGGY 2 cut(s) 121, 140
BssECI CCNNGG 3 cut(s) 187, 334, 374
BssMI GATC 2 cut(s) 350, 402
BssT1I CCWWGG 2 cut(s) 187, 374
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 2 cut(s) 308, 575
Bst6I CTCTTC 2 cut(s) 200, 495
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 3 cut(s) 46, 94, 123
BstDEI CTNAG 4 cut(s) 88, 128, 135, 633
BstF5I GGATG 3 cut(s) 238, 448, 469
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 3 cut(s) 87, 127, 651
BstKTI GATC 2 cut(s) 353, 405
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 2 cut(s) 350, 402
BstMWI GCNNNNNNNGC 5 cut(s) 50, 93, 131, 289, 345
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 334, 424
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 351
BstV2I GAAGAC 1 cut(s) 324
BsuRI GGCC 3 cut(s) 17, 140, 211
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 3 cut(s) 238, 448, 469
Cac8I GCNNGC 3 cut(s) 46, 94, 123
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 3 cut(s) 87, 127, 651
Cfr10I RCCGGY 2 cut(s) 121, 140
Cfr13I GGNCC 1 cut(s) 304
CviAII CATG 2 cut(s) 262, 421
DdeI CTNAG 4 cut(s) 88, 128, 135, 633
DpnI GATC 2 cut(s) 352, 404
DpnII GATC 2 cut(s) 350, 402
DriI GACNNNNNGTC 1 cut(s) 262
Eam1104I CTCTTC 2 cut(s) 200, 495
Eam1105I GACNNNNNGTC 1 cut(s) 262
EarI CTCTTC 2 cut(s) 200, 495
Ecl136II GAGCTC 2 cut(s) 134, 601
Eco130I CCWWGG 2 cut(s) 187, 374
Eco147I AGGCCT 1 cut(s) 211
Eco24I GRGCYC 2 cut(s) 136, 603
Eco47I GGWCC 1 cut(s) 304
Eco53kI GAGCTC 2 cut(s) 134, 601
Eco57I CTGAAG 3 cut(s) 45, 520, 542
EcoICRI GAGCTC 2 cut(s) 134, 601
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 187, 374
EcoT38I GRGCYC 2 cut(s) 136, 603
ErhI CCWWGG 2 cut(s) 187, 374
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 2 cut(s) 265, 424
FaiI YATR 5 cut(s) 263, 281, 326, 422, 479
FaqI GGGAC 2 cut(s) 205, 392
FatI CATG 2 cut(s) 261, 420
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 340
FokI GGATG 3 cut(s) 245, 455, 476
FriOI GRGCYC 2 cut(s) 136, 603
Fsp4HI GCNGC 1 cut(s) 340
FspBI CTAG 2 cut(s) 213, 219
GlaI GCGC 3 cut(s) 86, 126, 650
GluI GCNGC 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 3 cut(s) 17, 140, 211
HapII CCGG 3 cut(s) 122, 141, 336
HhaI GCGC 3 cut(s) 87, 127, 651
Hin1II CATG 2 cut(s) 265, 424
Hin6I GCGC 3 cut(s) 85, 125, 649
HinP1I GCGC 3 cut(s) 85, 125, 649
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 193, 429
HpaII CCGG 3 cut(s) 122, 141, 336
HphI GGTGA 6 cut(s) 18, 24, 96, 231, 258, 497
Hpy166II GTNNAC 3 cut(s) 116, 160, 592
Hpy188I TCNGA 1 cut(s) 386
Hpy188III TCNNGA 3 cut(s) 354, 566, 604
Hpy8I GTNNAC 3 cut(s) 116, 160, 592
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 111, 566
HpyCH4III ACNGT 2 cut(s) 308, 575
HpyCH4IV ACGT 2 cut(s) 112, 162
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 93, 131, 289, 345
HpyF3I CTNAG 4 cut(s) 88, 128, 135, 633
HpySE526I ACGT 2 cut(s) 112, 162
Hsp92II CATG 2 cut(s) 265, 424
HspAI GCGC 3 cut(s) 85, 125, 649
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 2 cut(s) 350, 402
LmnI GCTCC 3 cut(s) 376, 531, 646
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 2 cut(s) 279, 505
MaeI CTAG 2 cut(s) 213, 219
MaeII ACGT 2 cut(s) 112, 162
MaeIII GTNAC 3 cut(s) 182, 264, 575
MalI GATC 2 cut(s) 352, 404
MboI GATC 2 cut(s) 350, 402
MboII GAAGA 3 cut(s) 217, 329, 512
MhlI GDGCHC 3 cut(s) 136, 373, 603
MlyI GAGTC 2 cut(s) 187, 423
MnlI CCTC 7 cut(s) 28, 201, 267, 350, 355, 433, 454
MroNI GCCGGC 1 cut(s) 121
MseI TTAA 1 cut(s) 171
MspI CCGG 3 cut(s) 122, 141, 336
MspR9I CCNGG 2 cut(s) 336, 426
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 5 cut(s) 50, 93, 131, 289, 345
NaeI GCCGGC 1 cut(s) 123
NciI CCSGG 1 cut(s) 336
NdeII GATC 2 cut(s) 350, 402
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 265, 424
NmuCI GTSAC 2 cut(s) 264, 575
NspI RCATGY 1 cut(s) 424
NspV TTCGAA 1 cut(s) 315
PceI AGGCCT 1 cut(s) 211
PdiI GCCGGC 1 cut(s) 123
PkrI GCNGC 1 cut(s) 341
PleI GAGTC 2 cut(s) 187, 423
PpsI GAGTC 2 cut(s) 187, 423
Ppu21I YACGTR 1 cut(s) 113
PsiI TTATAA 1 cut(s) 479
Psp124BI GAGCTC 2 cut(s) 136, 603
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspPI GGNCC 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 292
SacI GAGCTC 2 cut(s) 136, 603
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 2 cut(s) 350, 402
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 2 cut(s) 187, 423
ScrFI CCNGG 2 cut(s) 336, 426
SduI GDGCHC 3 cut(s) 136, 373, 603
SfaNI GCATC 2 cut(s) 279, 505
SfuI TTCGAA 1 cut(s) 315
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SseBI AGGCCT 1 cut(s) 211
SsiI CCGC 1 cut(s) 412
SspMI CTAG 2 cut(s) 213, 219
SstI GAGCTC 2 cut(s) 136, 603
StuI AGGCCT 1 cut(s) 211
StyD4I CCNGG 2 cut(s) 334, 424
StyI CCWWGG 2 cut(s) 187, 374
TaaI ACNGT 2 cut(s) 308, 575
TaiI ACGT 2 cut(s) 115, 165
TaqI TCGA 1 cut(s) 315
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TseFI GTSAC 2 cut(s) 264, 575
TseI GCWGC 1 cut(s) 339
Tsp45I GTSAC 2 cut(s) 264, 575
VpaK11BI GGWCC 1 cut(s) 304
XceI RCATGY 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 2 cut(s) 213, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.