Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014041

Physical Location & Seq

Genomic Coordinates Forward (+)

31842732 .. 31848051

Gene Structure 5320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1228800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGGATACAAAAGTACTCTCCTCCGGAGTCATATACACCAACTTGCCGGAAAGCTATGTCAGACCAGAATCCGACCGGCCTCGCTTGTCCGAAGTCTCTGAATGCGAAAACATTCCCGTTATTGACCTGGCTTCTCCCAACAGAGCCCAAACCGTGCAGCAAGTTGGCGATGCCTGCAAATATTATGGTTTTTTTCAGGTTATTAACCATGGGGTGTCAACGGAAGGAGTGGAAAAAATGGTAGGGGTGGCCAATGAGTTTTTTAACCTTCCTGTGGAGGAAAAATTGAAGCTGTACTCAGATGACCCCTCAAAAACCATGAGACTTTCAACAAGTTTTAATGTAAAGAAGGAGAAGGTTCATAACTGGAGAGATTATCTTCGCCTCCATTGCTATCCTTTGGACAAGTACGTTCCTGAGTGGCCCTCCAATCCTTCGTCCTTCAGGGACACTGTGGGTAATTACTGTAGAGAGGTTCGAGAACTCGGATACAGATTAGAGGAATTAGTATCAGAAAGTTTAGGGCTGGAGAAAGATTACATAAGGAGCACATTGGGCGAGCAAGGACAGCACATGGCGGTCAACTTTTACCCGCCATGTCCGCAGCCCGAGCTGACTTACGGGCTGCCGGCCCACACCGACCCAAATGCCCTAACAATTCTGCTCCAAGACCTTGGAGTTGCTGGCCTTCAAGTTCTCAAAGATGGCAAATGGGTTGCTGTCAATCCCCAACCTAATGCCTTTGTCATTAACATTGGTGACCAATTACAGGCTCTAAGTAATGGGATTTACAAGAGTGTGTGGCATCGGGCCATTACCAATACTGAAAGACCAAGGCTCTCAGTAGCTTCATTCTTCTGCCCACAAGACGATGCTCTAATAAGCCCAGCAAAAGCTCTCACAGAGGATGGATCTGCAGCCATATATAGGGGATACACCTACACTGAGTATTATAAGAAGTTTTGGACTCGGGACTTGGAGAAAGACCATTGTTTGGACCTTTTCAGGACTCAGCAGCTGCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002229 GO:0002239 GO:0003674 GO:0003824 GO:0004497 GO:0006082 GO:0006725 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009696 GO:0009719 GO:0009725 GO:0009751 GO:0009812 GO:0009813 GO:0009987 GO:0010033 GO:0010150 GO:0010817 GO:0014070 GO:0016054 GO:0016491 GO:0016705 GO:0016706 GO:0016709 GO:0016999 GO:0017001 GO:0017144 GO:0018958 GO:0019336 GO:0019439 GO:0019752 GO:0032501 GO:0032502 GO:0032787 GO:0033759 GO:0034785 GO:0042221 GO:0042440 GO:0042445 GO:0042447 GO:0042493 GO:0042537 GO:0042737 GO:0042742 GO:0043207 GO:0043436 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0046148 GO:0046244 GO:0046395 GO:0046677 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051213 GO:0051552 GO:0051553 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072329 GO:0090693 GO:0098542 GO:0099402 GO:1901360 GO:1901361 GO:1901575 GO:1901576 GO:1901615 GO:1901616 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

342

Amino Acids

38.79

Weight (kDa)

5.49

Isoelectric Point (pI)

41.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	38 - 143	2.8e-29	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	190 - 288	1.2e-35	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014041)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24530
fragaria_vesca	FvH4_7g32980
malus_domestica	MD01G1228800.v1.1 MD07G1299900.v1.1
prunus_persica	Prupe.2G319600_v2.0.a1
pyrus_communis	pycom01g23680 pycom07g27220
rosa_chinensis	RchiOBHm_Chr1g0382951
rosa_laevigata	RLG00000026146
rosa_multiflora	Rmu_sc0018693.1_g000007
rosa_roxburghii	Rroxscaffold_4G00277750
rosa_rugosa	Rorug01G0437300
rosa_samantha	Rh1AG462800 Rh1BG419800 Rh1CG433600 Rh1DG451800
rosa_wichuraiana	Rw1G040920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	954
AccB7I	CCANNNNNTGG	1 cut(s)	994
AccIII	TCCGGA	1 cut(s)	23
AciI	CCGC	3 cut(s)	578, 593, 602
AclWI	GGATC	1 cut(s)	919
AcoI	YGGCCR	1 cut(s)	249
AcuI	CTGAAG	1 cut(s)	427
AfaI	GTAC	3 cut(s)	15, 296, 410
AfiI	CCNNNNNNNGG	4 cut(s)	274, 769, 927, 994
AgsI	TTSAA	3 cut(s)	289, 330, 692
AjnI	CCWGG	1 cut(s)	126
AluBI	AGCT	6 cut(s)	54, 292, 613, 848, 896, 1018
AluI	AGCT	6 cut(s)	54, 292, 613, 848, 896, 1018
Alw21I	GWGCWC	1 cut(s)	551
Alw26I	GTCTC	2 cut(s)	100, 316
AlwI	GGATC	1 cut(s)	919
AlwNI	CAGNNNCTG	1 cut(s)	1018
Ama87I	CYCGRG	2 cut(s)	608, 969
Aor13HI	TCCGGA	1 cut(s)	23
AoxI	GGCC	6 cut(s)	77, 249, 422, 630, 685, 810
ApeKI	GCWGC	6 cut(s)	157, 604, 625, 917, 1015, 1018
Asp700I	GAANNNNTTC	1 cut(s)	111
AspS9I	GGNCC	4 cut(s)	423, 631, 810, 997
AsuHPI	GGTGA	1 cut(s)	770
AvaI	CYCGRG	2 cut(s)	608, 969
AvaII	GGWCC	1 cut(s)	997
BaeI	ACNNNNGTAYC	1 cut(s)	30
BalI	TGGCCA	1 cut(s)	251
BanII	GRGCYC	1 cut(s)	148
Bbv12I	GWGCWC	1 cut(s)	551
BbvI	GCAGC	5 cut(s)	169, 612, 616, 929, 1005
BccI	CCATC	2 cut(s)	698, 902
BcgI	CGANNNNNNTGC	2 cut(s)	629, 663
BciT130I	CCWGG	1 cut(s)	128
BciVI	GTATCC	2 cut(s)	482, 926
BcoDI	GTCTC	2 cut(s)	100, 316
BfaI	CTAG	1 cut(s)	1024
BfmI	CTRYAG	2 cut(s)	466, 915
BfuI	GTATCC	2 cut(s)	482, 926
BisI	GCNGC	6 cut(s)	158, 605, 626, 918, 1016, 1019
BlsI	GCNGC	6 cut(s)	159, 606, 627, 919, 1017, 1020
BmcAI	AGTACT	1 cut(s)	15
Bme1390I	CCNGG	1 cut(s)	128
Bme18I	GGWCC	1 cut(s)	997
BmeT110I	CYCGRG	2 cut(s)	608, 969
BmgT120I	GGNCC	4 cut(s)	423, 631, 810, 997
BmrFI	CCNGG	1 cut(s)	128
BmsI	GCATC	3 cut(s)	160, 814, 862
BplI	GAGNNNNNCTC	2 cut(s)	410, 442
BpmI	CTGGAG	2 cut(s)	388, 548
BsaJI	CCNNGG	3 cut(s)	208, 673, 833
BsaWI	WCCGGW	1 cut(s)	23
Bsc4I	CCNNNNNNNGG	4 cut(s)	274, 769, 927, 994
Bse118I	RCCGGY	2 cut(s)	75, 628
Bse1I	ACTGG	1 cut(s)	371
Bse3DI	GCAATG	1 cut(s)	388
BseAI	TCCGGA	1 cut(s)	23
BseBI	CCWGG	1 cut(s)	128
BseDI	CCNNGG	3 cut(s)	208, 673, 833
BseGI	GGATG	1 cut(s)	913
BseLI	CCNNNNNNNGG	4 cut(s)	274, 769, 927, 994
BseMI	GCAATG	1 cut(s)	388
BseMII	CTCAG	5 cut(s)	312, 408, 855, 936, 1025
BseNI	ACTGG	1 cut(s)	371
BseRI	GAGGAG	1 cut(s)	10
BseXI	GCAGC	5 cut(s)	169, 612, 616, 929, 1005
BseYI	CCCAGC	1 cut(s)	886
BsgI	GTGCAG	1 cut(s)	176
Bsh1285I	CGRYCG	1 cut(s)	76
BshFI	GGCC	6 cut(s)	79, 251, 424, 632, 687, 812
BsiEI	CGRYCG	1 cut(s)	76
BsiHKAI	GWGCWC	1 cut(s)	551
BsiHKCI	CYCGRG	2 cut(s)	608, 969
BsiSI	CCGG	4 cut(s)	24, 47, 76, 629
BslFI	GGGAC	2 cut(s)	461, 986
BslI	CCNNNNNNNGG	4 cut(s)	274, 769, 927, 994
BsmAI	GTCTC	2 cut(s)	100, 316
BsmFI	GGGAC	2 cut(s)	461, 986
BsmI	GAATGC	1 cut(s)	107
BsnI	GGCC	6 cut(s)	79, 251, 424, 632, 687, 812
BsoBI	CYCGRG	2 cut(s)	608, 969
Bsp1286I	GDGCHC	2 cut(s)	148, 551
Bsp13I	TCCGGA	1 cut(s)	23
Bsp143I	GATC	1 cut(s)	911
Bsp19I	CCATGG	1 cut(s)	208
BspACI	CCGC	3 cut(s)	578, 593, 602
BspANI	GGCC	6 cut(s)	79, 251, 424, 632, 687, 812
BspCNI	CTCAG	5 cut(s)	311, 409, 854, 937, 1024
BspEI	TCCGGA	1 cut(s)	23
BspMAI	CTGCAG	1 cut(s)	919
BspPI	GGATC	1 cut(s)	919
BsrDI	GCAATG	1 cut(s)	388
BsrFI	RCCGGY	2 cut(s)	75, 628
BsrI	ACTGG	1 cut(s)	371
BssAI	RCCGGY	2 cut(s)	75, 628
BssECI	CCNNGG	3 cut(s)	208, 673, 833
BssMI	GATC	1 cut(s)	911
BssT1I	CCWWGG	3 cut(s)	208, 673, 833
Bst2UI	CCWGG	1 cut(s)	128
Bst4CI	ACNGT	3 cut(s)	154, 454, 467
BstC8I	GCNNGC	4 cut(s)	175, 560, 630, 685
BstDEI	CTNAG	6 cut(s)	298, 417, 776, 841, 945, 1011
BstDSI	CCRYGG	1 cut(s)	208
BstEII	GGTNACC	1 cut(s)	758
BstF5I	GGATG	1 cut(s)	913
BstKTI	GATC	1 cut(s)	914
BstMAI	GTCTC	2 cut(s)	100, 316
BstMBI	GATC	1 cut(s)	911
BstMCI	CGRYCG	1 cut(s)	76
BstMWI	GCNNNNNNNGC	6 cut(s)	174, 390, 555, 568, 601, 610
BstNI	CCWGG	1 cut(s)	128
BstPI	GGTNACC	1 cut(s)	758
BstSCI	CCNGG	1 cut(s)	126
BstSFI	CTRYAG	2 cut(s)	466, 915
BstV1I	GCAGC	5 cut(s)	169, 612, 616, 929, 1005
BstX2I	RGATCY	1 cut(s)	911
BstXI	CCANNNNNNTGG	1 cut(s)	674
BstYI	RGATCY	1 cut(s)	911
BsuI	GTATCC	2 cut(s)	482, 926
BsuRI	GGCC	6 cut(s)	79, 251, 424, 632, 687, 812
BtgI	CCRYGG	1 cut(s)	208
BtgZI	GCGATG	1 cut(s)	183
BtsCI	GGATG	1 cut(s)	913
BtsIMutI	CAGTG	2 cut(s)	450, 942
Cac8I	GCNNGC	4 cut(s)	175, 560, 630, 685
CaiI	CAGNNNCTG	1 cut(s)	1018
Cfr10I	RCCGGY	2 cut(s)	75, 628
Cfr13I	GGNCC	4 cut(s)	423, 631, 810, 997
Csp6I	GTAC	3 cut(s)	14, 295, 409
CviAII	CATG	4 cut(s)	209, 319, 574, 597
CviQI	GTAC	3 cut(s)	14, 295, 409
DdeI	CTNAG	6 cut(s)	298, 417, 776, 841, 945, 1011
DpnI	GATC	1 cut(s)	913
DpnII	GATC	1 cut(s)	911
EaeI	YGGCCR	1 cut(s)	249
Eco130I	CCWWGG	3 cut(s)	208, 673, 833
Eco24I	GRGCYC	1 cut(s)	148
Eco47I	GGWCC	1 cut(s)	997
Eco57I	CTGAAG	1 cut(s)	427
Eco88I	CYCGRG	2 cut(s)	608, 969
Eco91I	GGTNACC	1 cut(s)	758
EcoO65I	GGTNACC	1 cut(s)	758
EcoRII	CCWGG	1 cut(s)	126
EcoT14I	CCWWGG	3 cut(s)	208, 673, 833
EcoT38I	GRGCYC	1 cut(s)	148
ErhI	CCWWGG	3 cut(s)	208, 673, 833
FaeI	CATG	4 cut(s)	212, 322, 577, 600
FaqI	GGGAC	2 cut(s)	461, 986
FatI	CATG	4 cut(s)	208, 318, 573, 596
FauI	CCCGC	1 cut(s)	600
Fnu4HI	GCNGC	6 cut(s)	158, 605, 626, 918, 1016, 1019
FokI	GGATG	1 cut(s)	920
FriOI	GRGCYC	1 cut(s)	148
Fsp4HI	GCNGC	6 cut(s)	158, 605, 626, 918, 1016, 1019
FspBI	CTAG	1 cut(s)	1024
GluI	GCNGC	6 cut(s)	158, 605, 626, 918, 1016, 1019
GsaI	CCCAGC	1 cut(s)	890
GsuI	CTGGAG	2 cut(s)	388, 548
HaeIII	GGCC	6 cut(s)	79, 251, 424, 632, 687, 812
HapII	CCGG	4 cut(s)	24, 47, 76, 629
Hin1II	CATG	4 cut(s)	212, 322, 577, 600
HincII	GTYRAC	2 cut(s)	219, 583
HindII	GTYRAC	2 cut(s)	219, 583
HinfI	GANTC	4 cut(s)	27, 68, 967, 1009
HpaII	CCGG	4 cut(s)	24, 47, 76, 629
HphI	GGTGA	1 cut(s)	770
Hpy166II	GTNNAC	2 cut(s)	219, 583
Hpy188I	TCNGA	7 cut(s)	62, 73, 91, 100, 301, 488, 514
Hpy188III	TCNNGA	5 cut(s)	24, 416, 479, 971, 1006
Hpy8I	GTNNAC	2 cut(s)	219, 583
HpyAV	CCTTC	7 cut(s)	218, 278, 343, 349, 444, 451, 698
HpyCH4III	ACNGT	3 cut(s)	154, 454, 467
HpyCH4IV	ACGT	1 cut(s)	411
HpyCH4V	TGCA	3 cut(s)	157, 177, 917
HpyF10VI	GCNNNNNNNGC	6 cut(s)	174, 390, 555, 568, 601, 610
HpyF3I	CTNAG	6 cut(s)	298, 417, 776, 841, 945, 1011
HpySE526I	ACGT	1 cut(s)	411
Hsp92II	CATG	4 cut(s)	212, 322, 577, 600
Kpn2I	TCCGGA	1 cut(s)	23
KroI	GCCGGC	1 cut(s)	628
KroNI	GCCGGC	1 cut(s)	630
Kzo9I	GATC	1 cut(s)	911
LmnI	GCTCC	2 cut(s)	546, 669
Lsp1109I	GCAGC	5 cut(s)	169, 612, 616, 929, 1005
LweI	GCATC	3 cut(s)	160, 814, 862
MaeI	CTAG	1 cut(s)	1024
MaeII	ACGT	1 cut(s)	411
MaeIII	GTNAC	1 cut(s)	758
MalI	GATC	1 cut(s)	913
MboI	GATC	1 cut(s)	911
MboII	GAAGA	2 cut(s)	371, 847
MflI	RGATCY	1 cut(s)	911
MhlI	GDGCHC	2 cut(s)	148, 551
MlsI	TGGCCA	1 cut(s)	251
MluCI	AATT	5 cut(s)	284, 460, 503, 657, 764
MluNI	TGGCCA	1 cut(s)	251
MlyI	GAGTC	3 cut(s)	36, 961, 1003
MmeI	TCCRAC	1 cut(s)	96
MnlI	CCTC	9 cut(s)	31, 90, 271, 319, 395, 436, 466, 493, 898
Mox20I	TGGCCA	1 cut(s)	251
MroI	TCCGGA	1 cut(s)	23
MroNI	GCCGGC	1 cut(s)	628
MroXI	GAANNNNTTC	1 cut(s)	111
MscI	TGGCCA	1 cut(s)	251
MseI	TTAA	4 cut(s)	204, 264, 339, 750
Msp20I	TGGCCA	1 cut(s)	251
MspA1I	CMGCKG	1 cut(s)	1018
MspI	CCGG	4 cut(s)	24, 47, 76, 629
MspR9I	CCNGG	1 cut(s)	128
Mva1269I	GAATGC	1 cut(s)	107
MvaI	CCWGG	1 cut(s)	128
MwoI	GCNNNNNNNGC	6 cut(s)	174, 390, 555, 568, 601, 610
NaeI	GCCGGC	1 cut(s)	630
NcoI	CCATGG	1 cut(s)	208
NdeII	GATC	1 cut(s)	911
NgoMIV	GCCGGC	1 cut(s)	628
NlaIII	CATG	4 cut(s)	212, 322, 577, 600
NmuCI	GTSAC	1 cut(s)	758
PctI	GAATGC	1 cut(s)	107
PdiI	GCCGGC	1 cut(s)	630
PdmI	GAANNNNTTC	1 cut(s)	111
PfeI	GAWTC	1 cut(s)	68
PflMI	CCANNNNNTGG	1 cut(s)	994
PkrI	GCNGC	6 cut(s)	159, 606, 627, 919, 1017, 1020
PleI	GAGTC	3 cut(s)	35, 961, 1003
PpsI	GAGTC	3 cut(s)	35, 961, 1003
PsiI	TTATAA	1 cut(s)	954
Psp6I	CCWGG	1 cut(s)	126
PspEI	GGTNACC	1 cut(s)	758
PspFI	CCCAGC	1 cut(s)	886
PspGI	CCWGG	1 cut(s)	126
PspPI	GGNCC	4 cut(s)	423, 631, 810, 997
PstI	CTGCAG	1 cut(s)	919
PstNI	CAGNNNCTG	1 cut(s)	1018
PsuI	RGATCY	1 cut(s)	911
PvuII	CAGCTG	1 cut(s)	1018
RsaI	GTAC	3 cut(s)	15, 296, 410
RsaNI	GTAC	3 cut(s)	14, 295, 409
SaqAI	TTAA	4 cut(s)	204, 264, 339, 750
SatI	GCNGC	6 cut(s)	158, 605, 626, 918, 1016, 1019
Sau3AI	GATC	1 cut(s)	911
Sau96I	GGNCC	4 cut(s)	423, 631, 810, 997
ScaI	AGTACT	1 cut(s)	15
SchI	GAGTC	3 cut(s)	36, 961, 1003
ScrFI	CCNGG	1 cut(s)	128
SduI	GDGCHC	2 cut(s)	148, 551
SfaNI	GCATC	3 cut(s)	160, 814, 862
SfcI	CTRYAG	2 cut(s)	466, 915
SinI	GGWCC	1 cut(s)	997
Sse9I	AATT	5 cut(s)	284, 460, 503, 657, 764
SsiI	CCGC	3 cut(s)	578, 593, 602
SspI	AATATT	1 cut(s)	182
SspMI	CTAG	1 cut(s)	1024
StyD4I	CCNGG	1 cut(s)	126
StyI	CCWWGG	3 cut(s)	208, 673, 833
TaaI	ACNGT	3 cut(s)	154, 454, 467
TaiI	ACGT	1 cut(s)	414
TaqI	TCGA	1 cut(s)	478
TasI	AATT	5 cut(s)	284, 460, 503, 657, 764
TatI	WGTACW	2 cut(s)	13, 294
TfiI	GAWTC	1 cut(s)	68
Tru1I	TTAA	4 cut(s)	204, 264, 339, 750
Tru9I	TTAA	4 cut(s)	204, 264, 339, 750
TscAI	CASTG	2 cut(s)	457, 949
TseFI	GTSAC	1 cut(s)	758
TseI	GCWGC	6 cut(s)	157, 604, 625, 917, 1015, 1018
Tsp45I	GTSAC	1 cut(s)	758
TspDTI	ATGAA	2 cut(s)	350, 840
TspGWI	ACGGA	1 cut(s)	236
TspRI	CASTG	2 cut(s)	457, 949
Van91I	CCANNNNNTGG	1 cut(s)	994
VpaK11BI	GGWCC	1 cut(s)	997
XmnI	GAANNNNTTC	1 cut(s)	111
XspI	CTAG	1 cut(s)	1024
ZrmI	AGTACT	1 cut(s)	15

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.