Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014041

Physical Location & Seq

Genomic Coordinates Forward (+)

35881165 .. 35889422

Gene Structure 8258 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1299900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGGATGCAAAAGTCCTCTCCTCCGGAGTCAGACACTCCAACGTGCCGGAAAGCTATGTCAGACCAGAATCCGACCGGCCTGGCTTGTCCGAAGTGTCTGAATGCCAAAACATTCCAGTCATTGACCTGGCTTCTCCTAACAGAGCCCAAACTGTGCAGCAAATTGGCGATGCTTGCAAAAATTATGGCTTTTTTCAGGTTATTAATCATGGGGTGTCAACGGAAGGAGTGGAAAAAATGGTAGGGGTGGCCAATGAGTTTTTTAGCCTTCCTGTGGAGGAAAAGTTGAAGTTGTACTCAGATGATCCCTCAAAAACCATGAGACTTTCAACAAGTTTTAATGTGAACAAGGAGAAGGTTCATAACTGGAGAGATTATCTTCGACTCCATTGCTATCCTTTGGACAAGTACGTTCCTGAGTGGCCCTCCAATCCTTCTTCCTTCAAGGACACTGTGAGTAGTTACTGCACAGAGGTTCGAGAACTTGGATACAGGTTACAGGAATTAATATCAGAGAGTTTAGGGCTGGAGAAAGATTACATAAAGAGCGCACTGGGCGAGCAAGGACAGCACATGGCGGTGAACTTTTACCCGCCGTGTCCCCAGCCCGAGCTGACTTATGGACTGCCGGGCCACACCGACCCAAATGCCCTAACAATTTTGCTCCAAGACCTTGGAGTTGCTGGCCTTCAAGTTCTCAAAGATGGCAAATGGGTTGCTGTCAATCCCCACCCTAATGCCTTTGTCATTAACGTTGGTGACCAATTACAGGCCCTAAGTAATGGAATTTACAAGAGTGTGTGGCATCGAGCCGTTACCAATACTGAGAGAGCAAGACTCTCAGTAGCTTCATTCCTCTGCCCACAAGACGATGCTCTAATAAGCCCAGCAAAAGCTCTCACAGAGGATGGATCTGCAGCCATATATAGGGGATACACTTATACTGAGTATTATAAGAAGTTTTGGACTCGGGACTTGGAGAAAGATCATTGTTTGGACCTTTTCAAGATCCAGCAACAGCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002229 GO:0002239 GO:0003674 GO:0003824 GO:0004497 GO:0006082 GO:0006725 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009696 GO:0009719 GO:0009725 GO:0009751 GO:0009812 GO:0009813 GO:0009987 GO:0010033 GO:0010150 GO:0010817 GO:0014070 GO:0016054 GO:0016491 GO:0016705 GO:0016706 GO:0016709 GO:0016999 GO:0017001 GO:0017144 GO:0018958 GO:0019336 GO:0019439 GO:0019752 GO:0032501 GO:0032502 GO:0032787 GO:0033759 GO:0034785 GO:0042221 GO:0042440 GO:0042445 GO:0042447 GO:0042493 GO:0042537 GO:0042737 GO:0042742 GO:0043207 GO:0043436 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0046148 GO:0046244 GO:0046395 GO:0046677 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051213 GO:0051552 GO:0051553 GO:0051704 GO:0051707 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072329 GO:0090693 GO:0098542 GO:0099402 GO:1901360 GO:1901361 GO:1901575 GO:1901576 GO:1901615 GO:1901616 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

342

Amino Acids

38.35

Weight (kDa)

5.64

Isoelectric Point (pI)

42.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	38 - 143	9e-30	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	190 - 288	3.5e-35	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014041)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G24530
fragaria_vesca	FvH4_7g32980
malus_domestica	MD01G1228800.v1.1 MD07G1299900.v1.1
prunus_persica	Prupe.2G319600_v2.0.a1
pyrus_communis	pycom01g23680 pycom07g27220
rosa_chinensis	RchiOBHm_Chr1g0382951
rosa_laevigata	RLG00000026146
rosa_multiflora	Rmu_sc0018693.1_g000007
rosa_roxburghii	Rroxscaffold_4G00277750
rosa_rugosa	Rorug01G0437300
rosa_samantha	Rh1AG462800 Rh1BG419800 Rh1CG433600 Rh1DG451800
rosa_wichuraiana	Rw1G040920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	954
AccIII	TCCGGA	1 cut(s)	23
AciI	CCGC	2 cut(s)	578, 593
AclI	AACGTT	1 cut(s)	753
AclWI	GGATC	3 cut(s)	299, 919, 1003
AcoI	YGGCCR	1 cut(s)	249
AcsI	RAATTY	1 cut(s)	786
AfaI	GTAC	2 cut(s)	296, 410
AfiI	CCNNNNNNNGG	3 cut(s)	274, 769, 927
AgsI	TTSAA	5 cut(s)	289, 330, 445, 692, 1006
AjnI	CCWGG	2 cut(s)	79, 126
AluBI	AGCT	4 cut(s)	54, 613, 848, 896
AluI	AGCT	4 cut(s)	54, 613, 848, 896
Alw26I	GTCTC	1 cut(s)	316
AlwI	GGATC	3 cut(s)	299, 919, 1003
Ama87I	CYCGRG	2 cut(s)	608, 969
Aor13HI	TCCGGA	1 cut(s)	23
AoxI	GGCC	6 cut(s)	77, 249, 422, 631, 685, 771
ApeKI	GCWGC	2 cut(s)	157, 917
ApoI	RAATTY	1 cut(s)	786
AseI	ATTAAT	2 cut(s)	204, 506
AspLEI	GCGC	1 cut(s)	551
AspS9I	GGNCC	4 cut(s)	423, 631, 772, 997
AsuC2I	CCSGG	1 cut(s)	630
AsuHPI	GGTGA	2 cut(s)	592, 770
AvaI	CYCGRG	2 cut(s)	608, 969
AvaII	GGWCC	1 cut(s)	997
BalI	TGGCCA	1 cut(s)	251
BanII	GRGCYC	1 cut(s)	148
BbvI	GCAGC	2 cut(s)	169, 929
BccI	CCATC	2 cut(s)	698, 902
BceAI	ACGGC	2 cut(s)	580, 797
BcgI	CGANNNNNNTGC	4 cut(s)	372, 406, 629, 663
BciT130I	CCWGG	2 cut(s)	81, 128
BciVI	GTATCC	2 cut(s)	482, 926
BcnI	CCSGG	1 cut(s)	630
BcoDI	GTCTC	1 cut(s)	316
BfaI	CTAG	1 cut(s)	1024
BfmI	CTRYAG	1 cut(s)	915
BfuI	GTATCC	2 cut(s)	482, 926
BisI	GCNGC	2 cut(s)	158, 918
BlsI	GCNGC	2 cut(s)	159, 919
Bme1390I	CCNGG	3 cut(s)	81, 128, 630
Bme18I	GGWCC	1 cut(s)	997
BmeT110I	CYCGRG	2 cut(s)	608, 969
BmgT120I	GGNCC	4 cut(s)	423, 631, 772, 997
BmrFI	CCNGG	3 cut(s)	81, 128, 630
BmrI	ACTGGG	1 cut(s)	563
BmsI	GCATC	3 cut(s)	160, 814, 862
BmuI	ACTGGG	1 cut(s)	563
BplI	GAGNNNNNCTC	4 cut(s)	410, 442, 822, 854
BpmI	CTGGAG	2 cut(s)	388, 548
BpuMI	CCSGG	1 cut(s)	630
BsaJI	CCNNGG	1 cut(s)	673
BsaWI	WCCGGW	1 cut(s)	23
BsaXI	ACNNNNNCTCC	2 cut(s)	269, 299
Bsc4I	CCNNNNNNNGG	3 cut(s)	274, 769, 927
Bse118I	RCCGGY	1 cut(s)	75
Bse1I	ACTGG	3 cut(s)	116, 371, 558
Bse3DI	GCAATG	1 cut(s)	388
BseAI	TCCGGA	1 cut(s)	23
BseBI	CCWGG	2 cut(s)	81, 128
BseDI	CCNNGG	1 cut(s)	673
BseGI	GGATG	2 cut(s)	10, 913
BseLI	CCNNNNNNNGG	3 cut(s)	274, 769, 927
BseMI	GCAATG	1 cut(s)	388
BseMII	CTCAG	5 cut(s)	312, 408, 816, 855, 936
BseNI	ACTGG	3 cut(s)	116, 371, 558
BseRI	GAGGAG	1 cut(s)	10
BseXI	GCAGC	2 cut(s)	169, 929
BseYI	CCCAGC	2 cut(s)	603, 886
BsgI	GTGCAG	2 cut(s)	176, 451
Bsh1285I	CGRYCG	1 cut(s)	76
BshFI	GGCC	6 cut(s)	79, 251, 424, 633, 687, 773
BsiEI	CGRYCG	1 cut(s)	76
BsiHKCI	CYCGRG	2 cut(s)	608, 969
BsiSI	CCGG	4 cut(s)	24, 47, 76, 629
BslFI	GGGAC	2 cut(s)	585, 986
BslI	CCNNNNNNNGG	3 cut(s)	274, 769, 927
BsmAI	GTCTC	1 cut(s)	316
BsmFI	GGGAC	2 cut(s)	585, 986
BsmI	GAATGC	1 cut(s)	107
BsnI	GGCC	6 cut(s)	79, 251, 424, 633, 687, 773
BsoBI	CYCGRG	2 cut(s)	608, 969
Bsp1286I	GDGCHC	1 cut(s)	148
Bsp13I	TCCGGA	1 cut(s)	23
Bsp143I	GATC	4 cut(s)	304, 911, 985, 1008
BspACI	CCGC	2 cut(s)	578, 593
BspANI	GGCC	6 cut(s)	79, 251, 424, 633, 687, 773
BspCNI	CTCAG	5 cut(s)	311, 409, 817, 854, 937
BspEI	TCCGGA	1 cut(s)	23
BspMAI	CTGCAG	1 cut(s)	919
BspPI	GGATC	3 cut(s)	299, 919, 1003
BsrDI	GCAATG	1 cut(s)	388
BsrFI	RCCGGY	1 cut(s)	75
BsrI	ACTGG	3 cut(s)	116, 371, 558
BssAI	RCCGGY	1 cut(s)	75
BssECI	CCNNGG	1 cut(s)	673
BssMI	GATC	4 cut(s)	304, 911, 985, 1008
BssT1I	CCWWGG	1 cut(s)	673
Bst2UI	CCWGG	2 cut(s)	81, 128
Bst4CI	ACNGT	2 cut(s)	154, 454
BstC8I	GCNNGC	3 cut(s)	175, 560, 685
BstDEI	CTNAG	6 cut(s)	298, 417, 776, 825, 841, 945
BstEII	GGTNACC	1 cut(s)	758
BstF5I	GGATG	2 cut(s)	10, 913
BstHHI	GCGC	1 cut(s)	551
BstKTI	GATC	4 cut(s)	307, 914, 988, 1011
BstMAI	GTCTC	1 cut(s)	316
BstMBI	GATC	4 cut(s)	304, 911, 985, 1008
BstMCI	CGRYCG	1 cut(s)	76
BstMWI	GCNNNNNNNGC	3 cut(s)	174, 555, 568
BstNI	CCWGG	2 cut(s)	81, 128
BstPI	GGTNACC	1 cut(s)	758
BstSCI	CCNGG	3 cut(s)	79, 126, 628
BstSFI	CTRYAG	1 cut(s)	915
BstV1I	GCAGC	2 cut(s)	169, 929
BstX2I	RGATCY	2 cut(s)	911, 1008
BstXI	CCANNNNNNTGG	1 cut(s)	674
BstYI	RGATCY	2 cut(s)	911, 1008
BsuI	GTATCC	2 cut(s)	482, 926
BsuRI	GGCC	6 cut(s)	79, 251, 424, 633, 687, 773
BtgZI	GCGATG	1 cut(s)	183
BtsCI	GGATG	2 cut(s)	10, 913
BtsIMutI	CAGTG	2 cut(s)	450, 551
Cac8I	GCNNGC	3 cut(s)	175, 560, 685
CfoI	GCGC	1 cut(s)	551
Cfr10I	RCCGGY	1 cut(s)	75
Cfr13I	GGNCC	4 cut(s)	423, 631, 772, 997
Csp6I	GTAC	2 cut(s)	295, 409
CviAII	CATG	3 cut(s)	209, 319, 574
CviQI	GTAC	2 cut(s)	295, 409
DdeI	CTNAG	6 cut(s)	298, 417, 776, 825, 841, 945
DpnI	GATC	4 cut(s)	306, 913, 987, 1010
DpnII	GATC	4 cut(s)	304, 911, 985, 1008
EaeI	YGGCCR	1 cut(s)	249
Eco130I	CCWWGG	1 cut(s)	673
Eco24I	GRGCYC	1 cut(s)	148
Eco47I	GGWCC	1 cut(s)	997
Eco88I	CYCGRG	2 cut(s)	608, 969
Eco91I	GGTNACC	1 cut(s)	758
EcoO109I	RGGNCCY	1 cut(s)	772
EcoO65I	GGTNACC	1 cut(s)	758
EcoRII	CCWGG	2 cut(s)	79, 126
EcoT14I	CCWWGG	1 cut(s)	673
EcoT38I	GRGCYC	1 cut(s)	148
ErhI	CCWWGG	1 cut(s)	673
FaeI	CATG	3 cut(s)	212, 322, 577
FaqI	GGGAC	2 cut(s)	585, 986
FatI	CATG	3 cut(s)	208, 318, 573
FauI	CCCGC	1 cut(s)	600
Fnu4HI	GCNGC	2 cut(s)	158, 918
FokI	GGATG	2 cut(s)	17, 920
FriOI	GRGCYC	1 cut(s)	148
Fsp4HI	GCNGC	2 cut(s)	158, 918
FspBI	CTAG	1 cut(s)	1024
GlaI	GCGC	1 cut(s)	550
GluI	GCNGC	2 cut(s)	158, 918
GsaI	CCCAGC	2 cut(s)	607, 890
GsuI	CTGGAG	2 cut(s)	388, 548
HaeIII	GGCC	6 cut(s)	79, 251, 424, 633, 687, 773
HapII	CCGG	4 cut(s)	24, 47, 76, 629
HhaI	GCGC	1 cut(s)	551
Hin1II	CATG	3 cut(s)	212, 322, 577
Hin6I	GCGC	1 cut(s)	549
HinP1I	GCGC	1 cut(s)	549
HincII	GTYRAC	1 cut(s)	219
HindII	GTYRAC	1 cut(s)	219
HinfI	GANTC	5 cut(s)	27, 68, 384, 837, 967
HpaII	CCGG	4 cut(s)	24, 47, 76, 629
HphI	GGTGA	2 cut(s)	592, 770
Hpy166II	GTNNAC	3 cut(s)	219, 346, 583
Hpy188I	TCNGA	7 cut(s)	32, 62, 73, 91, 100, 301, 514
Hpy188III	TCNNGA	5 cut(s)	24, 416, 479, 971, 1006
Hpy8I	GTNNAC	3 cut(s)	219, 346, 583
HpyAV	CCTTC	6 cut(s)	218, 278, 349, 444, 451, 698
HpyCH4III	ACNGT	2 cut(s)	154, 454
HpyCH4IV	ACGT	3 cut(s)	42, 411, 753
HpyCH4V	TGCA	5 cut(s)	8, 157, 177, 468, 917
HpyF10VI	GCNNNNNNNGC	3 cut(s)	174, 555, 568
HpyF3I	CTNAG	6 cut(s)	298, 417, 776, 825, 841, 945
HpySE526I	ACGT	3 cut(s)	42, 411, 753
Hsp92II	CATG	3 cut(s)	212, 322, 577
HspAI	GCGC	1 cut(s)	549
Kpn2I	TCCGGA	1 cut(s)	23
Kzo9I	GATC	4 cut(s)	304, 911, 985, 1008
LmnI	GCTCC	1 cut(s)	669
Lsp1109I	GCAGC	2 cut(s)	169, 929
LweI	GCATC	3 cut(s)	160, 814, 862
MaeI	CTAG	1 cut(s)	1024
MaeII	ACGT	3 cut(s)	42, 411, 753
MaeIII	GTNAC	4 cut(s)	461, 495, 758, 814
MalI	GATC	4 cut(s)	306, 913, 987, 1010
MboI	GATC	4 cut(s)	304, 911, 985, 1008
MboII	GAAGA	2 cut(s)	371, 429
MflI	RGATCY	2 cut(s)	911, 1008
MhlI	GDGCHC	1 cut(s)	148
MlsI	TGGCCA	1 cut(s)	251
MluCI	AATT	6 cut(s)	162, 181, 503, 657, 764, 786
MluNI	TGGCCA	1 cut(s)	251
MlyI	GAGTC	4 cut(s)	36, 378, 831, 961
MmeI	TCCRAC	2 cut(s)	63, 96
MnlI	CCTC	8 cut(s)	26, 31, 271, 319, 436, 466, 866, 898
Mox20I	TGGCCA	1 cut(s)	251
MroI	TCCGGA	1 cut(s)	23
MscI	TGGCCA	1 cut(s)	251
MseI	TTAA	4 cut(s)	204, 339, 506, 750
MslI	CAYNNNNRTG	2 cut(s)	578, 735
Msp20I	TGGCCA	1 cut(s)	251
MspI	CCGG	4 cut(s)	24, 47, 76, 629
MspR9I	CCNGG	3 cut(s)	81, 128, 630
Mva1269I	GAATGC	1 cut(s)	107
MvaI	CCWGG	2 cut(s)	81, 128
MwoI	GCNNNNNNNGC	3 cut(s)	174, 555, 568
NciI	CCSGG	1 cut(s)	630
NdeII	GATC	4 cut(s)	304, 911, 985, 1008
NlaIII	CATG	3 cut(s)	212, 322, 577
NmuCI	GTSAC	1 cut(s)	758
PctI	GAATGC	1 cut(s)	107
PfeI	GAWTC	1 cut(s)	68
PkrI	GCNGC	2 cut(s)	159, 919
PleI	GAGTC	4 cut(s)	35, 378, 831, 961
PpsI	GAGTC	4 cut(s)	35, 378, 831, 961
PshBI	ATTAAT	2 cut(s)	204, 506
PsiI	TTATAA	1 cut(s)	954
Psp1406I	AACGTT	1 cut(s)	753
Psp6I	CCWGG	2 cut(s)	79, 126
PspEI	GGTNACC	1 cut(s)	758
PspFI	CCCAGC	2 cut(s)	603, 886
PspGI	CCWGG	2 cut(s)	79, 126
PspPI	GGNCC	4 cut(s)	423, 631, 772, 997
PstI	CTGCAG	1 cut(s)	919
PsuI	RGATCY	2 cut(s)	911, 1008
RsaI	GTAC	2 cut(s)	296, 410
RsaNI	GTAC	2 cut(s)	295, 409
RseI	CAYNNNNRTG	2 cut(s)	578, 735
SaqAI	TTAA	4 cut(s)	204, 339, 506, 750
SatI	GCNGC	2 cut(s)	158, 918
Sau3AI	GATC	4 cut(s)	304, 911, 985, 1008
Sau96I	GGNCC	4 cut(s)	423, 631, 772, 997
SchI	GAGTC	4 cut(s)	36, 378, 831, 961
ScrFI	CCNGG	3 cut(s)	81, 128, 630
SduI	GDGCHC	1 cut(s)	148
SfaNI	GCATC	3 cut(s)	160, 814, 862
SfcI	CTRYAG	1 cut(s)	915
SinI	GGWCC	1 cut(s)	997
SmiMI	CAYNNNNRTG	2 cut(s)	578, 735
Sse9I	AATT	6 cut(s)	162, 181, 503, 657, 764, 786
SsiI	CCGC	2 cut(s)	578, 593
SspMI	CTAG	1 cut(s)	1024
StyD4I	CCNGG	3 cut(s)	79, 126, 628
StyI	CCWWGG	1 cut(s)	673
TaaI	ACNGT	2 cut(s)	154, 454
TaiI	ACGT	3 cut(s)	45, 414, 756
TaqI	TCGA	3 cut(s)	382, 478, 808
TasI	AATT	6 cut(s)	162, 181, 503, 657, 764, 786
TatI	WGTACW	1 cut(s)	294
TfiI	GAWTC	1 cut(s)	68
Tru1I	TTAA	4 cut(s)	204, 339, 506, 750
Tru9I	TTAA	4 cut(s)	204, 339, 506, 750
TscAI	CASTG	2 cut(s)	457, 558
TseFI	GTSAC	1 cut(s)	758
TseI	GCWGC	2 cut(s)	157, 917
Tsp45I	GTSAC	1 cut(s)	758
TspDTI	ATGAA	2 cut(s)	350, 840
TspGWI	ACGGA	1 cut(s)	236
TspRI	CASTG	2 cut(s)	457, 558
VpaK11BI	GGWCC	1 cut(s)	997
VspI	ATTAAT	2 cut(s)	204, 506
XapI	RAATTY	1 cut(s)	786
XspI	CTAG	1 cut(s)	1024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.