MD01G1231600.v1.1

cellular response to sulfur starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009112
Physical Location & Seq
Reverse (-)
32041962 .. 32042288
327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1231600.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCGGTAACGAAGCAACAGCCGGCGGCGGAAGAAGAGAAGCTGCTGAGGCAGAGGAACGAGGAATTGGAGAGGGAGTTGAGGAAGAGCCAGGAGAGGGAGGAGAGGATGAAGGCGGAGCTCCAGAGGGCCCGCGAAAGGCTCCGAGTCGCCGAGGAGGCGGAGGAGCGCCTCTGCTCGCAGCTCGGTGAGCTCGAAGCCGAGGCAGTCGATCAGGCGCGTATGGATCATGCCCGGATTCTGGCCCTCGTGGACAAGCTCTCGCAGGCCCAGCGCCTCCTCCAGGCTTCCGCCGTTGCCCTGCCGCCGGGACTCGCTTCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

12.37

Weight (kDa)

5.6

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSU PF24980 13 - 69 2.6e-08 Response to Low Sulfur (LSU) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 135, 220
AciI CCGC 8 cut(s) 5, 26, 29, 116, 133, 161, 291, 305
AclWI GGATC 1 cut(s) 234
AfiI CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 307
AjnI CCWGG 2 cut(s) 90, 282
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AluBI AGCT 5 cut(s) 43, 121, 184, 193, 259
AluI AGCT 5 cut(s) 43, 121, 184, 193, 259
Alw21I GWGCWC 2 cut(s) 123, 195
AlwI GGATC 1 cut(s) 234
AoxI GGCC 3 cut(s) 129, 243, 267
ApaI GGGCCC 1 cut(s) 133
ApeKI GCWGC 2 cut(s) 43, 181
AspLEI GCGC 3 cut(s) 171, 220, 276
AspS9I GGNCC 4 cut(s) 129, 130, 244, 268
AsuC2I CCSGG 2 cut(s) 235, 309
AsuHPI GGTGA 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 3 cut(s) 123, 133, 195
BauI CACGAG 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 123, 195
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 2 cut(s) 30, 193
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 92, 284
BcnI CCSGG 2 cut(s) 235, 309
BfoI RGCGCY 2 cut(s) 172, 277
BglI GCCNNNNNGGC 1 cut(s) 158
BisI GCNGC 4 cut(s) 27, 44, 182, 305
BlsI GCNGC 4 cut(s) 28, 45, 183, 306
Bme1390I CCNGG 4 cut(s) 92, 235, 284, 309
BmgT120I GGNCC 4 cut(s) 129, 130, 244, 268
BmiI GGNNCC 2 cut(s) 131, 143
BmrFI CCNGG 4 cut(s) 92, 235, 284, 309
BpmI CTGGAG 2 cut(s) 107, 266
Bpu10I CCTNAGC 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 235, 309
BsaJI CCNNGG 2 cut(s) 153, 201
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 307
Bse118I RCCGGY 1 cut(s) 22
BseBI CCWGG 2 cut(s) 92, 284
BseDI CCNNGG 2 cut(s) 153, 201
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 307
BseMII CTCAG 1 cut(s) 38
BseRI GAGGAG 4 cut(s) 116, 170, 179, 269
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 2 cut(s) 30, 193
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 2 cut(s) 135, 220
BshFI GGCC 3 cut(s) 131, 245, 269
BsiHKAI GWGCWC 2 cut(s) 123, 195
BsiSI CCGG 3 cut(s) 23, 235, 308
BslI CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 307
BsnI GGCC 3 cut(s) 131, 245, 269
Bsp120I GGGCCC 1 cut(s) 129
Bsp1286I GDGCHC 3 cut(s) 123, 133, 195
Bsp143I GATC 2 cut(s) 211, 226
BspACI CCGC 8 cut(s) 5, 26, 29, 116, 133, 161, 291, 305
BspANI GGCC 3 cut(s) 131, 245, 269
BspCNI CTCAG 1 cut(s) 39
BspFNI CGCG 2 cut(s) 135, 220
BspLI GGNNCC 2 cut(s) 131, 143
BspPI GGATC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 153, 201
BssMI GATC 2 cut(s) 211, 226
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 92, 284
Bst6I CTCTTC 2 cut(s) 30, 80
BstC8I GCNNGC 4 cut(s) 24, 133, 179, 267
BstDEI CTNAG 1 cut(s) 47
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 2 cut(s) 135, 220
BstH2I RGCGCY 2 cut(s) 172, 277
BstHHI GCGC 3 cut(s) 171, 220, 276
BstKTI GATC 2 cut(s) 214, 229
BstMBI GATC 2 cut(s) 211, 226
BstMWI GCNNNNNNNGC 4 cut(s) 49, 158, 190, 271
BstNI CCWGG 2 cut(s) 92, 284
BstSCI CCNGG 4 cut(s) 90, 233, 282, 307
BstSLI GKGCMC 1 cut(s) 133
BstUI CGCG 2 cut(s) 135, 220
BstV1I GCAGC 2 cut(s) 30, 193
BsuRI GGCC 3 cut(s) 131, 245, 269
BtsCI GGATG 1 cut(s) 114
Cac8I GCNNGC 4 cut(s) 24, 133, 179, 267
CfoI GCGC 3 cut(s) 171, 220, 276
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 4 cut(s) 129, 130, 244, 268
CviAII CATG 1 cut(s) 230
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 213, 228
DpnII GATC 2 cut(s) 211, 226
Eam1104I CTCTTC 2 cut(s) 30, 80
EarI CTCTTC 2 cut(s) 30, 80
EciI GGCGGA 4 cut(s) 44, 131, 176, 280
Ecl136II GAGCTC 2 cut(s) 121, 193
Eco24I GRGCYC 3 cut(s) 123, 133, 195
Eco53kI GAGCTC 2 cut(s) 121, 193
EcoICRI GAGCTC 2 cut(s) 121, 193
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 90, 282
EcoT38I GRGCYC 3 cut(s) 123, 133, 195
FaeI CATG 1 cut(s) 233
FaiI YATR 2 cut(s) 224, 231
FatI CATG 1 cut(s) 229
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 4 cut(s) 27, 44, 182, 305
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 3 cut(s) 123, 133, 195
Fsp4HI GCNGC 4 cut(s) 27, 44, 182, 305
GlaI GCGC 3 cut(s) 170, 219, 275
GluI GCNGC 4 cut(s) 27, 44, 182, 305
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 2 cut(s) 107, 266
HaeII RGCGCY 2 cut(s) 172, 277
HaeIII GGCC 3 cut(s) 131, 245, 269
HapII CCGG 3 cut(s) 23, 235, 308
HhaI GCGC 3 cut(s) 171, 220, 276
Hin1II CATG 1 cut(s) 233
Hin6I GCGC 3 cut(s) 169, 218, 274
HinP1I GCGC 3 cut(s) 169, 218, 274
HinfI GANTC 3 cut(s) 147, 238, 312
HpaII CCGG 3 cut(s) 23, 235, 308
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 158, 190, 271
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 1 cut(s) 233
HspAI GCGC 3 cut(s) 169, 218, 274
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Kzo9I GATC 2 cut(s) 211, 226
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 4 cut(s) 118, 126, 147, 166
Lsp1109I GCAGC 2 cut(s) 30, 193
MaeIII GTNAC 1 cut(s) 7
MalI GATC 2 cut(s) 213, 228
MboI GATC 2 cut(s) 211, 226
MboII GAAGA 3 cut(s) 44, 47, 97
MhlI GDGCHC 3 cut(s) 123, 133, 195
MluCI AATT 1 cut(s) 65
MlyI GAGTC 2 cut(s) 156, 306
MroNI GCCGGC 1 cut(s) 22
MspI CCGG 3 cut(s) 23, 235, 308
MspR9I CCNGG 4 cut(s) 92, 235, 284, 309
MvaI CCWGG 2 cut(s) 92, 284
MvnI CGCG 2 cut(s) 135, 220
MwoI GCNNNNNNNGC 4 cut(s) 49, 158, 190, 271
NaeI GCCGGC 1 cut(s) 24
NciI CCSGG 2 cut(s) 235, 309
NdeII GATC 2 cut(s) 211, 226
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 1 cut(s) 233
NlaIV GGNNCC 2 cut(s) 131, 143
NmeAIII GCCGAG 2 cut(s) 178, 226
PciSI GCTCTTC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 4 cut(s) 28, 45, 183, 306
PleI GAGTC 2 cut(s) 155, 306
PpsI GAGTC 2 cut(s) 155, 306
Psp124BI GAGCTC 2 cut(s) 123, 195
Psp6I CCWGG 2 cut(s) 90, 282
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 2 cut(s) 90, 282
PspN4I GGNNCC 2 cut(s) 131, 143
PspOMI GGGCCC 1 cut(s) 129
PspPI GGNCC 4 cut(s) 129, 130, 244, 268
SacI GAGCTC 2 cut(s) 123, 195
SapI GCTCTTC 1 cut(s) 80
SatI GCNGC 4 cut(s) 27, 44, 182, 305
Sau3AI GATC 2 cut(s) 211, 226
Sau96I GGNCC 4 cut(s) 129, 130, 244, 268
SchI GAGTC 2 cut(s) 156, 306
ScrFI CCNGG 4 cut(s) 92, 235, 284, 309
SduI GDGCHC 3 cut(s) 123, 133, 195
SetI ASST 5 cut(s) 45, 123, 186, 195, 261
Sse9I AATT 1 cut(s) 65
SsiI CCGC 8 cut(s) 5, 26, 29, 116, 133, 161, 291, 305
SstI GAGCTC 2 cut(s) 123, 195
StyD4I CCNGG 4 cut(s) 90, 233, 282, 307
TaqI TCGA 2 cut(s) 195, 210
TasI AATT 1 cut(s) 65
TauI GCSGC 2 cut(s) 29, 307
TfiI GAWTC 1 cut(s) 238
TseI GCWGC 2 cut(s) 43, 181
TspDTI ATGAA 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.