MD07G1303600.v1.1

cellular response to sulfur starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009112
Physical Location & Seq
Reverse (-)
36122614 .. 36122943
330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1303600.v1.1.491

Sequence Viewer

Length: 330 bp
ATGGCGGTAACGAAGCAACTGCCGGCGGCGGAAGAAGAGAAGCTGCTGAGGCAGAGGAACGAGGAGTTGGAGAGGGAGTTGAGGAAAAGCCAGGAGAGGGAGGAGCGGATGAAGGCGGAGCTCCAGAGAGCCCGCGAGAGGCTCCGAGTCGCCGAGGAGGCAGAAGAGCGTCTCTGCTCTCAGCTCGGCGAGCTCGAAGCCGAGGCCGTCGATCAGGCGCGTATGGATCACGGCCGAATTCTGGCCCTCGCGGACCAGCTCTCGCACGCCCAGCGCCTCCTCCAGGCTTCCGCCATTCCCCTGCCGTCGTCGGGACTCGCTTCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

12.44

Weight (kDa)

5.53

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSU PF24980 13 - 69 2.5e-08 Response to Low Sulfur (LSU) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 106
AccII CGCG 3 cut(s) 135, 220, 251
AciI CCGC 8 cut(s) 5, 26, 29, 106, 116, 133, 251, 291
AclWI GGATC 1 cut(s) 234
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 1 cut(s) 237
AfiI CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 311
AjnI CCWGG 2 cut(s) 90, 282
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AluBI AGCT 5 cut(s) 43, 121, 184, 193, 259
AluI AGCT 5 cut(s) 43, 121, 184, 193, 259
Alw21I GWGCWC 2 cut(s) 123, 195
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 234
AoxI GGCC 3 cut(s) 204, 232, 243
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 1 cut(s) 237
AspLEI GCGC 2 cut(s) 220, 276
AspS9I GGNCC 2 cut(s) 244, 253
AvaII GGWCC 1 cut(s) 253
BanII GRGCYC 3 cut(s) 123, 133, 195
Bbv12I GWGCWC 2 cut(s) 123, 195
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 30
BceAI ACGGC 3 cut(s) 191, 247, 289
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 2 cut(s) 92, 284
BcoDI GTCTC 1 cut(s) 176
BfoI RGCGCY 1 cut(s) 277
BglI GCCNNNNNGGC 1 cut(s) 158
BisI GCNGC 2 cut(s) 27, 44
BlsI GCNGC 2 cut(s) 28, 45
Bme1390I CCNGG 2 cut(s) 92, 284
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 244, 253
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 92, 284
BpmI CTGGAG 2 cut(s) 107, 266
Bpu10I CCTNAGC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 153, 201
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 311
Bse118I RCCGGY 1 cut(s) 22
BseBI CCWGG 2 cut(s) 92, 284
BseDI CCNNGG 2 cut(s) 153, 201
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 311
BseMII CTCAG 2 cut(s) 38, 194
BseRI GAGGAG 4 cut(s) 77, 116, 170, 269
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 1 cut(s) 30
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 3 cut(s) 135, 220, 251
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 3 cut(s) 206, 234, 245
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 123, 195
BsiSI CCGG 1 cut(s) 23
BslI CCNNNNNNNGG 5 cut(s) 97, 138, 241, 283, 311
BsmAI GTCTC 1 cut(s) 176
BsmBI CGTCTC 1 cut(s) 176
BsnI GGCC 3 cut(s) 206, 234, 245
Bsp1286I GDGCHC 3 cut(s) 123, 133, 195
Bsp143I GATC 2 cut(s) 211, 226
BspACI CCGC 8 cut(s) 5, 26, 29, 106, 116, 133, 251, 291
BspANI GGCC 3 cut(s) 206, 234, 245
BspCNI CTCAG 2 cut(s) 39, 193
BspFNI CGCG 3 cut(s) 135, 220, 251
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 159
BsrBI CCGCTC 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 153, 201
BssMI GATC 2 cut(s) 211, 226
Bst2UI CCWGG 2 cut(s) 92, 284
Bst6I CTCTTC 2 cut(s) 30, 159
BstC8I GCNNGC 4 cut(s) 24, 133, 191, 267
BstDEI CTNAG 2 cut(s) 47, 180
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 3 cut(s) 135, 220, 251
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 2 cut(s) 220, 276
BstKTI GATC 2 cut(s) 214, 229
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 211, 226
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 4 cut(s) 49, 158, 190, 271
BstNI CCWGG 2 cut(s) 92, 284
BstSCI CCNGG 2 cut(s) 90, 282
BstUI CGCG 3 cut(s) 135, 220, 251
BstV1I GCAGC 1 cut(s) 30
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 3 cut(s) 206, 234, 245
BtsCI GGATG 1 cut(s) 114
Cac8I GCNNGC 4 cut(s) 24, 133, 191, 267
CfoI GCGC 2 cut(s) 220, 276
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 2 cut(s) 244, 253
CseI GACGC 1 cut(s) 158
DdeI CTNAG 2 cut(s) 47, 180
DpnI GATC 2 cut(s) 213, 228
DpnII GATC 2 cut(s) 211, 226
EaeI YGGCCR 1 cut(s) 232
EagI CGGCCG 1 cut(s) 232
Eam1104I CTCTTC 2 cut(s) 30, 159
EarI CTCTTC 2 cut(s) 30, 159
EciI GGCGGA 3 cut(s) 44, 131, 280
Ecl136II GAGCTC 2 cut(s) 121, 193
EclXI CGGCCG 1 cut(s) 232
Eco24I GRGCYC 3 cut(s) 123, 133, 195
Eco47I GGWCC 1 cut(s) 253
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 2 cut(s) 121, 193
EcoICRI GAGCTC 2 cut(s) 121, 193
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 2 cut(s) 90, 282
EcoT38I GRGCYC 3 cut(s) 123, 133, 195
Esp3I CGTCTC 1 cut(s) 176
FaiI YATR 1 cut(s) 224
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 2 cut(s) 27, 44
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 3 cut(s) 123, 133, 195
Fsp4HI GCNGC 2 cut(s) 27, 44
GlaI GCGC 2 cut(s) 219, 275
GluI GCNGC 2 cut(s) 27, 44
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 2 cut(s) 107, 266
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 3 cut(s) 206, 234, 245
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 158
HhaI GCGC 2 cut(s) 220, 276
Hin6I GCGC 2 cut(s) 218, 274
HinP1I GCGC 2 cut(s) 218, 274
HinfI GANTC 2 cut(s) 147, 315
HpaII CCGG 1 cut(s) 23
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 2 cut(s) 124, 312
Hpy99I CGWCG 3 cut(s) 212, 310, 313
HpyAV CCTTC 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 158, 190, 271
HpyF3I CTNAG 2 cut(s) 47, 180
HspAI GCGC 2 cut(s) 218, 274
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Kzo9I GATC 2 cut(s) 211, 226
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 4 cut(s) 103, 118, 126, 147
Lsp1109I GCAGC 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 7
MalI GATC 2 cut(s) 213, 228
MbiI CCGCTC 1 cut(s) 106
MboI GATC 2 cut(s) 211, 226
MboII GAAGA 3 cut(s) 44, 47, 176
MhlI GDGCHC 3 cut(s) 123, 133, 195
MluCI AATT 1 cut(s) 237
MlyI GAGTC 2 cut(s) 156, 309
MmeI TCCRAC 1 cut(s) 48
MroNI GCCGGC 1 cut(s) 22
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 92, 284
MvaI CCWGG 2 cut(s) 92, 284
MvnI CGCG 3 cut(s) 135, 220, 251
MwoI GCNNNNNNNGC 4 cut(s) 49, 158, 190, 271
NaeI GCCGGC 1 cut(s) 24
NdeII GATC 2 cut(s) 211, 226
NgoMIV GCCGGC 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 3 cut(s) 165, 178, 226
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 24
PkrI GCNGC 2 cut(s) 28, 45
PleI GAGTC 2 cut(s) 155, 309
PpsI GAGTC 2 cut(s) 155, 309
Psp124BI GAGCTC 2 cut(s) 123, 195
Psp6I CCWGG 2 cut(s) 90, 282
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 2 cut(s) 90, 282
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 2 cut(s) 244, 253
SacI GAGCTC 2 cut(s) 123, 195
SapI GCTCTTC 1 cut(s) 159
SatI GCNGC 2 cut(s) 27, 44
Sau3AI GATC 2 cut(s) 211, 226
Sau96I GGNCC 2 cut(s) 244, 253
SchI GAGTC 2 cut(s) 156, 309
ScrFI CCNGG 2 cut(s) 92, 284
SduI GDGCHC 3 cut(s) 123, 133, 195
SetI ASST 5 cut(s) 45, 123, 186, 195, 261
SinI GGWCC 1 cut(s) 253
Sse9I AATT 1 cut(s) 237
SsiI CCGC 8 cut(s) 5, 26, 29, 106, 116, 133, 251, 291
SstI GAGCTC 2 cut(s) 123, 195
StyD4I CCNGG 2 cut(s) 90, 282
TaqI TCGA 2 cut(s) 195, 210
TasI AATT 1 cut(s) 237
TauI GCSGC 1 cut(s) 29
TseI GCWGC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 253
XagI CCTNNNNNAGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.